| cherry virus a: cdna cloning of dsrna, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry. | the nucleotide sequence (7383 nucleotides) of a newly identified member of the genus capillovirus, cherry virus a (cva), was obtained from cdna clones. the cdna was generated from dsrna extracted from plant tissue infected with little cherry virus (lcv). small amounts of lcv dsrna served as template nucleic acid and enabled the construction of a library of which, unexpectedly, 7.5% of the recombinant plasmids were specific for cva. the genome organization of cva resembles that of apple stem groo ... | 1995 | 7636482 |
| complete nucleotide sequence of cherry virus a (cva) infecting sweet cherry in india. | cherry virus a (cva) is a graft-transmissible member of the genus capillovirus that infects different stone fruits. sweet cherry (prunus avium l; family rosaceae) is an important deciduous temperate fruit crop in the western himalayan region of india. in order to determine the health status of cherry plantations and the incidence of the virus in india, cherry orchards in the states of jammu and kashmir (j&k) and himachal pradesh (h.p.) were surveyed during the months of may and september 2009. t ... | 2010 | 20938696 |
| molecular characterization of a novel capillovirus from red currant. | the complete nucleotide sequence of a novel virus from red currant, provisionally named currant virus a (cuva), was determined. the genome is 7925 nucleotides long and has a 3'-poly(a) tail. the genome organization with two overlapping open reading frames is similar to that of capilloviruses, but the cuva genome is about 600 nucleotides longer than that of the longest known capillovirus, cherry virus a. the rna is predicted to encode a polyprotein with domains of methyltransferase, 2og-fe(ii) ox ... | 2016 | 26754736 |
| structure and genome organization of cherry virus a (capillovirus, betaflexiviridae) from china using small rna sequencing. | cherry virus a (cva) (capillovirus, betaflexiviridae) is widely present in cherry-growing areas. we obtained the complete genome of a cva isolate (cva-ta) using small rna deep sequencing, followed by overlapping reverse transcription-pcr (rt-pcr) and rapid amplification of cdna ends (race). the newly identified 5'-untranslated region (5'-utr) from cva-ta may form additional hairpin and loop structures to stabilize the cva genome. | 2016 | 27174277 |
| first complete genome sequence of cherry virus a. | the 5'-terminal genomic sequence of cherry virus a (cva) has long been unknown. we determined the first complete genome sequence of an apricot isolate of cva (7,434 nucleotides [nt]). the 5'-untranslated region was 107 nt in length, which was 53 nt longer than those of known cva sequences. | 2016 | 27284130 |
| nucleotide sequences of a korean isolate of apple stem grooving virus associated with black necrotic leaf spot disease on pear (pyrus pyrifolia). | pear black necrotic leaf spot (pbnls) is a disease of pears caused by capillovirus-like particles, which can be observed under the electron microscope. the disease was analyzed by western blot analysis with antisera raised against apple stem grooving virus (asgv) coat protein. cdnas covering the entire genome were synthesized by rt-pcr and race using rna isolated from chenopodium quinoa infected with sap extracted from pear leaves carrying black necrotic spot disease. the complete genome sequenc ... | 2004 | 15528995 |
| simultaneous detection and identification of four cherry viruses by two step multiplex rt-pcr with an internal control of plant nad5 mrna. | a multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed and standardized for the simultaneous detection of four cherry viruses: cherry virus a (cva, genus; capillovirus), cherry necrotic rusty mottle virus (cnrmv, unassigned species of the betaflexiviridae), little cherry virus 1 (lchv-1, genus; closterovirus) and prunus necrotic ringspot virus (pnrsv, genus; ilarvirus) with nad5 as plant internal control. a reliable and quick method for total plant rna extraction fro ... | 2013 | 23707922 |