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assessing date palm genetic diversity using different molecular markers.molecular marker technologies which rely on dna analysis provide powerful tools to assess biodiversity at different levels, i.e., among and within species. a range of different molecular marker techniques have been developed and extensively applied for detecting variability in date palm at the dna level. recently, the employment of gene-targeting molecular marker approaches to study biodiversity and genetic variations in many plant species has increased the attention of researchers interested in ...201728755220
date palm genetic diversity analysis using microsatellite polymorphism.date palm (phoenix dactylifera l.) is considered one of the great socioeconomic resources in the middle east and the arab regions. the tree has been and still is at the center of the comprehensive agricultural development. the number of known date palm cultivars, distributed worldwide, is approximately 3000. the success of genetic diversity conservation or any breeding program depends on an understanding of the amount and distribution of the genetic variation already in existence in the genetic ...201728755219
genetic diversity analysis of date palm using random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr).random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) are pcr-based molecular techniques used for evaluation and characterization of date palm to find the best genotype and male/female identification at an early stage. genetic fingerprinting using molecular markers is an important tool for the analysis of genetic diversity and cultivar identification. here, we present an improved dna extraction protocol using leaf tissue, based on the standard cetyltrimethyl ammonium br ...201728755218
molecular identification of fungal contamination in date palm tissue cultures.fungal contamination of in vitro cultures of date palm (phoenix dactylifera l.) is the major constraint to their initiation and maintenance. different molecular approaches have been applied successfully to analyze both inter- and intraspecific variation among fungal species as well as determine their identity. this chapter describes step-by-step procedures of molecular identification of fungal contaminants by internal transcribed spacer (its) products of the most common fungal contaminants of da ...201728755217
evaluation of clonal fidelity of micropropagated date palm by random amplified polymorphic dna (rapd).date palm is a fruit-bearing tree commonly found in arid and semiarid regions. it is a dioecious plant, producing fruit on female plants and a limited number of basal offshoots for propagation. to produce large numbers of uniform plantlets, tissue culture techniques are required. it is highly advisable to detect genetic variation that may occur through micropropagation techniques as it may lead to phenotypic alterations. random amplified polymorphic dna (rapd) is a simple and pcr-based molecular ...201728755216
encapsulation of date palm somatic embryos: synthetic seeds.synthetic seed or encapsulated somatic embryos may be used for propagation, storage, and exchange of plant germplasm and have many diverse applications in date palm cultivation. they have advantages over conventional use of offshoot material for germplasm propagation, maintenance, exchange, and transportation. this chapter describes a protocol for date palm synthetic seed production by encapsulation of somatic embryos with sodium alginate. among three concentrations used, 3% sodium alginate foll ...201728755215
in vitro conservation of date palm somatic embryos using growth-retardant conditions.in vitro conservation is carried out to maintain disease-free genetic materials, in a small area, protected against pests, insects, soil problems (alkaline, acidic, excess salinity, lack of organic matter, too dry, or too wet), climatic changes, and high-multiplication potential. a requirement of successful in vitro conservation is that the plants can be regenerated into complete plants rapidly when desired. the current work describes in vitro propagation and conservation techniques employing sl ...201728755214
in vitro conservation of date palm shoot-tip explants and callus cultures under minimal growth conditions.date palm fruit production has great economic significance for many countries. there is a fundamental necessity to conserve valuable date palm germplasm, but there are various problems with in vivo and ex situ conservation. in vitro storage has several advantages over conventional germplasm conservation methods. the in vitro technique offers a developed method of slow-growth storage, which is considered as an alternate solution for short- and medium-term storage of date palm germplasm under cont ...201728755213
in vitro cryopreservation of date palm caulogenic meristems.cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. in this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free ms medium. the positive impact of sucrose precul ...201728755212
cryopreservation of date palm pro-embryonic masses using the d cryo-plate technique.in this chapter, we describe a cryopreservation (liquid nitrogen, -196 °c) protocol developed for long-term storage of date palm pro-embryonic masses (pems), which uses the recently established d cryo-plate technique. clumps of pems (3-5 mm in size) were dissected from pem cultures and placed on pretreatment medium containing 171 g/l sucrose for 3 days. clumps were placed in the wells of aluminum cryo-plates in which they were made to adhere using droplets of 3% calcium alginate. pems were treat ...201728755211
in vitro conservation of date palm tissue cultures.in vitro technology offers a potential solution for the conservation of date palm germplasm. slow growth induced by low temperature allows storage from several months up to few years. otherwise, cryopreservation is suitable for long-term in vitro conservation, at between -79 and -196 °c. this chapter describes a protocol for cold storage at 5 °c and cryopreservation of date palm tissue cultures. for cold storage, 70% of shoot buds remain healthy after storing for 12 months at 5 °c, and callus cu ...201728755210
storage and viability assessment of date palm pollen.pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. here we describe pollen storage methods, such as room temperature (25-30 °c), refrigeration (4 °c), storage at 4 °c in desiccators, deep freezer (-20 °c), and cryopreservation (-196 °c). b ...201728755209
plant domestication: wild date palms illuminate a crop's sticky origins.in a new study, previously unknown populations of wild date palm have been identified in remote areas of oman. genomic analyses indicate date palm domestication occurred in the eastern portion of the arabian peninsula and reveal substantial subsequent gene flow with african palm populations.201728743015
the discovery of wild date palms in oman reveals a complex domestication history involving centers in the middle east and africa.for many crops, wild relatives constitute an extraordinary resource for cultivar improvement [1, 2] and also help to better understand the history of their domestication [3]. however, the wild ancestor species of several perennial crops have not yet been identified. perennial crops generally present a weak domestication syndrome allowing cultivated individuals to establish feral populations difficult to distinguish from truly wild populations, and there is frequently ongoing gene flow between wi ...201728712568
antioxidant system response and cdna-scot marker profiling in phoenix dactylifera l. plant under salinity stress.many phoenix dactylifera (date palm) cultivars are grown in the arid and semiarid regions of the world, including saudi arabia. p. dactylifera is highly tolerant to salinity stress. to investigate the response of khalas cultivar of p. dactylifera, two-month-old plants were treated with sodium chloride (50, 100, and 150 mm nacl) for three months. our result showed that proline content was higher in all treated plants compared to control plants. thiobarbituric acid reactive substances (tbars) were ...201728702461
the desert plant phoenix dactylifera closes stomata via nitrate-regulated slac1 anion channel.date palm phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. how are palms under such harsh environmental conditions able to limit transpirational water loss? here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from p. dactylifera. to understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast ab ...201728670699
synthetic co-attractants of the aggregation pheromone of the date palm root borer oryctes agamemnon.laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest oryctes agamemnon are reported. volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in olfactometers alone or combined with synthetic pheromone. a collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic p ...201728667548
cardiopreventive effect of ethanolic extract of date palm pollen against isoproterenol induced myocardial infarction in rats through the inhibition of the angiotensin-converting enzyme.the present study aimed to examine the putative preventive effect of the ethanolic extract date palm pollen (dpp, phoenix dactylifera l., family arecaceae) on isoproterenol-induced myocardial infarction (mi) in rats. twenty four rats were randomly divided into four groups including control. they were treated with dpp extract (400mg/kg) and clopidogrel (0.2mg/kg) for 7days followed by myocardial injury induction using subcutaneous isoproterenol (100mg/kg) with an interval of 24h for two days (6th ...201728645882
antibacterial activities of diospyros blancoi, phoenix dactylifera and morus nigra against dental caries causing pathogens: an in vitro study.the study was undertaken to determine the in-vitro antibacterial potential of diospyrosblancoi, phoenix dactylifera and morusnigra leaf extracts in hexane, chloroform, methanol, ethyl-acetate and aqueous extracts against dental caries causing bacteria. disc diffusion assay was used to determine the antibacterial efficacy; the extracts were further separated using thin layer chromatography and the anti-biofilm activity of the extracts was also determined. the preliminary phytochemical screening o ...201728603127
apparent metabolizable energy value of whole date palm (phoenix dactylifera l.) and its possible use as a feedstuff for aged laying hens.this study was conducted to evaluate the feeding value of whole date palm (wdp) with and without enzyme supplementation on aged laying hen's performance. apparent metabolizable energy value of wdp was determined by the total collection method using ten adult leghorn cockerels. wdp was substituted with a corn-soybean meal basal diet at 40% level, and then the metabolizable energy of this experimental diet and basal diet was determined. after that, a total number of 256 bovanz 95-week-old hens wer ...201728597141
detecting early signs of heat and drought stress in phoenix dactylifera (date palm).plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. here we studied the adaptation strategies in date palm, phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. in transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it ...201728570677
impact of environmental variables on dubas bug infestation rate: a case study from the sultanate of oman.date palm cultivation is economically important in the sultanate of oman, with significant financial investment coming from both the government and from private individuals. however, a global infestation of dubas bug (ommatissus lybicus bergevin) has impacted the middle east region, and infestations of date palms have been widespread. in this study, spatial analysis and geostatistical techniques were used to model the spatial distribution of dubas bug infestations to (a) identify correlations be ...201728558069
charting novel allergens from date palm pollen (phoenix sylvestris) using homology driven proteomics.pollen grains from phoenix sylvestris (date palm), a commonly cultivated tree in india has been found to cause severe allergic diseases in an increasing percentage of hypersensitive individuals. to unearth its allergenic components, pollen protein were profiled by two-dimensional gel electrophoresis followed by immunoblotting with date palm pollen sensitive patient sera. allergens were identified by maldi-tof/tof employing a layered proteomic approach combining conventional database dependent se ...201728535932
drdb: an online date palm genomic resource database.background: date palm (phoenix dactylifera l.) is a cultivated woody plant with agricultural and economic importance in many countries around the world. with the advantages of next generation sequencing technologies, genome sequences for many date palm cultivars have been released recently. short sequence repeat (ssr) and single nucleotide polymorphism (snp) can be identified from these genomic data, and have been proven to be very useful biomarkers in plant genome analysis and breeding. results ...201729209336
biochemical and genetical responses of phoenix dactylifera l. to cadmium stress.the cadmium (cd), a heavy metal, causes toxicity, which leads to hampering the growth and development of the plant. the molecular and biochemical approaches were used for the investigation of antioxidant system response and genotoxicity in date palm (phoenix dactylifera l.) cv. sagai in pot experiment having cd. the root length was more affected than the shoot length as more accumulation of cd occurs in roots. fresh weights of root and shoot were reduced significantly in treated plants as compar ...201729201916
abrogation of carbon tetrachloride-induced hepatotoxicity in sprague-dawley rats by ajwa date fruit extract through ameliorating oxidative stress and apoptosis.ajwa, a variety of date palme phoenix dactylifera l., has long been used and considered as one of the most popular fruits in the north africa and middle east region. for muslims this fruit is of religious importance and is mentioned several times in quran. besides being a part of the arabian essential diet, dates have been used traditionally for number of complications. this study aimed to evaluate the possible potential of ajwa date extract to guard against carbon tetrachloride (ccl4)-induced l ...201729175788
date palm biochar-polymer composites: an investigation of electrical, mechanical, thermal and rheological characteristics.the application of biochar (bc) as a filler in polymers can be viewed as a sustainable approach that incorporates pyrolysed waste based value-added material and simultaneously mitigate bio-waste in a smart way. the overarching aim of this work was to investigate the electrical, mechanical, thermal and rheological properties of biocomposite developed by utilizing date palm waste-derived bc for the reinforcing of polypropylene (pp) matrix. date palm waste derived bc prepared at (700 and 900°c) wer ...201729154049
evaluating the nutritional value of date pits and demonstrating their application in laying hen diets.this experiment was carried out to study the feeding value of ground date pits (dp) (phoenix dactylifera l.) with and without enzyme supplementation on laying hens' performance. apparent metabolizable energy value of dp was determined by the total collection method using 10 adult leghorn cockerels. after that, a total number of 144 lohmann 50-week-old lsl-lite hens were randomly allocated into six groups consisting of four replicates of six birds, based on a 3 × 2 factorial arrangement of the tr ...201729143376
process simulation and economic assessment of hydrothermal pretreatment and enzymatic hydrolysis of multi-feedstock lignocellulose - separate vs combined processing.biorefinery based on multi-feedstock lignocellulose can be viable where a sustainable supply of a single substrate is limited, for example in arid regions. processing of mixed feedstocks has been studied in lab scale, however, its economics are less studied. in this study, an economic comparison was made between separate and combined (mixed) processing approaches for multi-feedstock lignocellulose for the production of monomeric sugars. this modular approach of focusing on sugar platform makes t ...201729136939
physicochemical and sensory characteristics of spreadable liver pâtés with annatto extract (bixa orellana l.) and date palm co-products (phoenix dactylifera l.).two novel ingredients were incorporated into spreadable liver pâtés to study their effect on physicochemical and sensory characteristics and their possible use in the meat industry. fresh date (phoenix dactylifera, cv. confitera) co-products, as a paste (0, 2.5 and 7.5%), and annatto (bixa orellana) extract (0 and 128 mg/kg), as a colourant, and their combinations were incorporated into liver pâtés to study their effect on the final quality. the six formulations were analysed for chemical compos ...201729109376
effect of aqueous extract of tops of date palm leaves on blood glucose of diabetic rats.present study was carried out to examine the effect of tops of date palm leaves extract on blood glucose of streptozotocin induced diabetic rats. forty male sprague dawely rats (120-130g) were housed individually and randomly allocated to two main groups; diabetic group (n=30), and normal group (n=10) in the animal lab, faculty of agriculture and veterinary medicine, qassim university, saudi arabia. an aqueous extracts were prepared from tops of date palm leaves (edpl) and were orally administer ...201729105640
genome sequencing of microbacterium sp. yaish 1, a bacterial strain isolated from the rhizosphere of date palm trees affected by salinity.microbacterium sp. strain yaish 1 is a rhizospheric bacterium isolated from date palm orchards with high soil salinity. the genome was sequenced, and genes coding for growth-promoting 1-aminocyclopropane-1-carboxylate (acc) deaminase, siderophore-producing proteins, and tryptophan biosynthesis proteins were identified. here, we report the draft whole-genome sequencing of the strain.201729097476
correction to: date palm waste-derived biochar composites with silica and zeolite: synthesis, characterization and implication for carbon stability and recalcitrant potential.unfortunately, in the original publication of the article, prof. yong sik ok's affiliation was incorrectly published.201729282621
correction to: date palm waste biochars alter a soil respiration, microbial biomass carbon, and heavy metal mobility in contaminated mined soil.unfortunately, in the original publication of the article, prof. yang sik ok's affiliation was incorrectly published. the author's affiliation is as follows.201729282620
a vlp-based vaccine provides complete protection against nipah virus challenge following multiple-dose or single-dose vaccination schedules in a hamster model.nipah virus is a highly lethal zoonotic paramyxovirus that was first recognized in malaysia during an outbreak in 1998. during this outbreak, nipah virus infection caused a severe febrile neurological disease in humans who worked in close contact with infected pigs. the case fatality rate in humans was approximately 40%. since 2001, niv has re-emerged in bangladesh and india where fruit bats (pteropus spp.) have been identified as the principal reservoir of the virus. transmission to humans is c ...201729263876
identifying and controlling contamination of date palm tissue cultures.fungal and bacterial contaminations are major problems facing in vitro date palm (phoenix dactylifera l.) proliferation. to overcome this problem, we must first identify the fungal (e.g., alternaria sp., aspergillus niger, penicillium sp.) and bacterial (e.g., pseudomonas sp.) spread in date palm in vitro cultures. incorporating fungicides (e.g., copper oxychloride, vitavax t, and topsin m) or antibiotics (e.g., streptomycin, banocin, and bencid d) at 500 mg/l in medium significantly reduces the ...201728755344
the genome draft of coconut (cocos nucifera).coconut palm (cocos nucifera,2n = 32), a member of genus cocos and family arecaceae (palmaceae), is an important tropical fruit and oil crop. currently, coconut palm is cultivated in 93 countries, including central and south america, east and west africa, southeast asia and the pacific islands, with a total growth area of more than 12 million hectares [1]. coconut palm is generally classified into 2 main categories: "tall" (flowering 8-10 years after planting) and "dwarf" (flowering 4-6 years af ...201729048487
characterization and amplification of gene-based simple sequence repeat (ssr) markers in date palm.the paucity of molecular markers limits the application of genetic and genomic research in date palm (phoenix dactylifera l.). availability of expressed sequence tag (est) sequences in date palm may provide a good resource for developing gene-based markers. this study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (ssrs) from the est sequences in date palm. the est sequences studied are mainly homologous to those of elaeis guineensis and musa a ...201728755229
in silico approach for characterization and comparison of repeats in the genomes of oil and date palms.transposable elements (tes) are mobile genetic elements present in almost all eukaryotic genomes. due to their typical patterns of repetition, discovery, and characterization, they demand analysis by various bioinformatics software. probably, as a result of the need for a complex analysis, many genomes publicly available do not have these elements annotated yet. in this study, a de novo and homology-based identification of tes and microsatellites was performed using genomic data from 3 palm spec ...201728469420
report - screening of the anti-hyperglycemic activity of some medicinal plants of jordan.diabetes represents a group of common diseases that are characterized by dysregulation of blood glucose levels. plants are traditionally used for management of diseases including diabetes. in this study, we screened the anti-diabetic effect of extracts of 21 plants grown in jordan. extracts of plants were screened for their antihyperglycemic activity. diabetes was induced in sprague dawley rats using alloxan. plant extracts were dosed at 1gm/kg. blood glucose was measured at baseline and at ever ...201728653938
codon usage and codon pair patterns in non-grass monocot genomes.studies on codon usage in monocots have focused on grasses, and observed patterns of this taxon were generalized to all monocot species. here, non-grass monocot species were analysed to investigate the differences between grass and non-grass monocots.201729155926
the effect of increasing levels of date palm (phoenix dactylifera l.) seed on the performance, ruminal fermentation, antioxidant status and milk fatty acid profile of saanen dairy goats.this study was conducted to examine the effect of date palm (phoenix dactylifera l.) seed (dps) on the performance, ruminal fermentation, antioxidant status and milk fatty acid (fa) profile of dairy goats. eight multiparous saanen dairy goats, averaging 97 ± 7 days in milk (dim) and 2150 ± 130 g of milk production, were used in a 4 × 4 replicated latin square design. experimental diets contained 0% (control), 6% (dps6), 12% (dps12) and 18% (dps18) of dps. dry matter intake (dmi), milk production ...201728045194
physico-chemical characteristics and fungal profile of four saudi fresh date (phoenix dactylifera l.) cultivars.physico-chemical and microbial analyses of four commercial fresh date cultivars (sukari, barhi, khalas and rothana) grown in saudi arabia were evaluated. colorimetric assay indicated that sukari had the highest total sugar content of 13.21g/100gfw while the lowest was in rothana with 7.96g/100gfw. total phenolic content (tpc) ranged from 76.74 to 122.20mggae/100gfw in barhi and rothana respectively, whereas antioxidant activity was highest in sukari (105.99μggae/gfw) and lowest in khalas (90.81μ ...201727979253
transient gus gene expression in date palm fruit using agroinjection transformation technique.transient expression of foreign genes in plant tissue is a valuable tool for testing the efficacy of transformation methods. in this work, we present, for the first time, the utilization of agroinjection as an efficient transformation system for gene delivery in date palm fruit. the research utilized agrobacterium tumefaciens strain lba4404 harboring the binary vector pri201-an-gus carrying the beta-glucuronidase (gus) gene, under the control of a camv 35s and kanamycin (nptii) as an antibiotic ...201728755354
draft genome sequence of the endophytic bacillus aryabhattai strain squ-r12, identified from phoenix dactylifera l. roots.bacillus aryabhattai strain squ-r12 was isolated from date palm seedlings, where it showed a growth-promoting capacity by being able to synthesize indole-3-acetic acid phytohormone and reduce ethylene biosynthesis by producing 1-aminocyclopropane-1-carboxylic acid deaminase. the draft genome sequence of this strain is reported here.201728798173
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
mitochondrial molecular markers for resistance to bayoud disease in date palm.bayoud disease, caused by fusarium oxysporum f. sp. albedinis, is a very serious and destructive disease to date palm. screening of date palm germplasm for resistance to bayoud disease is a crucial step to avoid or alleviate the disease consequences. fortunately, it was discovered that there are two mitochondrial plasmid-like dna molecules associated with susceptibility or resistance to bayoud disease. in this chapter, we present a fast, simple, and reliable technique to screen date palm germpla ...201728755230
convergence of humans, bats, trees, and culture in nipah virus transmission, bangladesh.preventing emergence of new zoonotic viruses depends on understanding determinants for human risk. nipah virus (niv) is a lethal zoonotic pathogen that has spilled over from bats into human populations, with limited person-to-person transmission. we examined ecologic and human behavioral drivers of geographic variation for risk of niv infection in bangladesh. we visited 60 villages during 2011-2013 where cases of infection with niv were identified and 147 control villages. we compared case villa ...201728820130
a large-scale behavior change intervention to prevent nipah transmission in bangladesh: components and costs.nipah virus infection (niv) is a bat-borne zoonosis transmitted to humans through consumption of niv-contaminated raw date palm sap in bangladesh. the objective of this analysis was to measure the cost of an niv prevention intervention and estimate the cost of scaling it up to districts where spillover had been identified.201728651646
nipah virus outbreaks in bangladesh: a deadly infectious disease.during 2001-2011, multidisciplinary teams from the institute of epidemiology, disease control and research (iedcr) and international centre for diarrhoeal disease research, bangladesh(icddr,b) identified sporadic cases and 11 outbreaks of nipah encephalitis. three outbreaks were detected through sentinel surveillance; others were identified through event-based surveillance. a total of 196 cases of nipah encephalitis, in outbreaks, clusters and as isolated cases were detected from 20 districts of ...201728612796
illumina miseq sequencing analysis of fungal diversity in stored dates.date palm has been a major fruit tree in the middle east over thousands of years, especially in the arabian peninsula. dates are consumed fresh (rutab) or after partial drying and storage (tamar) during off-season. the aim of the study was to provide in-depth analysis of fungal communities associated with the skin (outer part) and mesocarp (inner fleshy part) of stored dates (tamar) of two cultivars (khenizi and burny) through the use of illumina miseq sequencing.201728347268
fungal root microbiome from healthy and brittle leaf diseased date palm trees (phoenix dactylifera l.) reveals a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites.in this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (bld) date palm trees (phoenix dactylifera l.) widely cultivated in arid zones of tunisia. a total of 52 endophytic fungi were isolated from healthy and bld roots of date palm trees, identified based on internal transcribed spacer-rdna sequence analysis and shown to represent 13 species belonging to fi ...201728293229
enzymatic saccharification and fermentation of cellulosic date palm wastes to glucose and lactic acid.the bioconversion of cellulosic wastes into high-value bio-products by saccharification and fermentation processes is an important step that can reduce the environmental pollution caused by agricultural wastes. in this study, enzymatic saccharification of treated and untreated date palm cellulosic wastes by the cellulases from geobacillus stearothermophilus was optimized. the alkaline pre-treatment of the date palm wastes was found to be effective in increasing the saccharification percentage. t ...201726887233
application of optimized large surface area date stone (phoenix dactylifera ) activated carbon for rhodamin b removal from aqueous solution: box-behnken design approach.box-behnken model of response surface methodology was used to study the effect of adsorption process parameters for rhodamine b (rhb) removal from aqueous solution through optimized large surface area date stone activated carbon. the set experiments with three input parameters such as time (10-600min), adsorbent dosage (0.5-10g/l) and temperature (25-50°c) were considered for statistical significance. the adequate relation was found between the input variables and response (removal percentage of ...201728167440
linking pollen quality and performance of neoseiulus californicus (acari: phytoseiidae) in two-spotted spider mite management programmes.it has been shown that pollen as a dietary supplement may increase the establishment of generalist predatory mites, and therefore pest control by these mites can be provided. life table studies were performed to evaluate the nutritional value of seven different pollens (almond, castor-bean, date-palm, maize, bitter-orange, sunflower and mixed bee pollen) as a supplementary food source for the spider mite predator neoseiulus californicus mcgregor. in addition, the nutritional quality of each poll ...201727117203
computational identification and comparative analysis of mirna precursors in three palm species.in the present study, mirna precursors in the genomes of three palm species were identified. analyzes of sequence conservation and biological function of their putative targets contribute to understand the roles of mirna in palm biology. micrornas are small rnas of 20-25 nucleotides in length, with important functions in the regulation of gene expression. recent genome sequencing of the palm species elaeis guineensis, elaeis oleifera and phoenix dactylifera have enabled the discovery of mirna ge ...201626919984
mold-ripened soft cheeses fortified with date palm fruit product as functional dairy products.date fruit based products are gaining popularity among the consumers in almost all date growing countries due to its added nutritional value. therefore, novel products were developed by combining two types of foods i.e., soft ripened cheeses and date fruit syrups or date powder. this study is the first to report the surface mold-ripened cheese production with date syrup and date powder. model cheeses were prepared from pasteurized milk inoculated with streptococcus thermophilus, penicillium came ...201626930796
antioxidants as a potential preventive and therapeutic strategy for cadmium.epidemiological studies provide a growing number of evidences that chronic exposure to relatively low levels of cadmium (cd), nowadays taking place in industrialized countries, may cause health hazard. thus, growing interest has been focused on effective ways of protection from adverse effects of exposure to this heavy metal. because numerous effects to cd's toxic action result from its prooxidative properties, it seems reasonable that special attention should be directed to agents that can prev ...201625944010
investigating rare risk factors for nipah virus in bangladesh: 2001-2012.human nipah encephalitis outbreaks have been identified almost yearly in bangladesh since 2001. though raw date palm sap consumption and person-to-person contact are recognized as major transmission pathways, alternative pathways of transmission are plausible and may not have been identified due to limited statistical power in each outbreak. we conducted a risk factor analysis using all 157 cases and 632 controls surveyed in previous investigations during 2004-2012 to identify exposures independ ...201627738775
it's not only what you say, it's also how you say it: communicating nipah virus prevention messages during an outbreak in bangladesh.during a fatal nipah virus (niv) outbreak in bangladesh, residents rejected biomedical explanations of niv transmission and treatment and lost trust in the public healthcare system. field anthropologists developed and communicated a prevention strategy to bridge the gap between the biomedical and local explanation of the outbreak.201627495927
nipah virus transmission from bats to humans associated with drinking traditional liquor made from date palm sap, bangladesh, 2011-2014.nipah virus (niv) is a paramyxovirus, and pteropus spp. bats are the natural reservoir. from december 2010 through march 2014, hospital-based encephalitis surveillance in bangladesh identified 18 clusters of niv infection. the source of infection for case-patients in 3 clusters in 2 districts was unknown. a team of epidemiologists and anthropologists investigated these 3 clusters comprising 14 case-patients, 8 of whom died. among the 14 case-patients, 8 drank fermented date palm sap (tari) regul ...201626981928
evolving epidemiology of nipah virus infection in bangladesh: evidence from outbreaks during 2010-2011.drinking raw date palm sap is the primary route of nipah virus (niv) transmission from bats to people in bangladesh; subsequent person-to-person transmission is common. during december 2010 to march 2011, we investigated niv epidemiology by interviewing cases using structured questionnaires, in-depth interviews, and group discussions to collect clinical and exposure histories. we conducted a case-control study to identify risk factors for transmission. we identified 43 cases; 23 were laboratory- ...201626122675
phenol-rich compounds sweet gel: a statistically more effective antibiotic than cloxacillin against pseudomonas aeruginosa.the purpose of this study was to obtain a natural antibiotic from phenol-rich compounds; for the dressing and the treatment of chronic wounds.201627695634
a pseudomonas strain isolated from date-palm rhizospheres improves root growth and promotes root formation in maize exposed to salt and aluminum stress.the aim of this study was to evaluate the effectiveness of pseudomonas fluorescens 002 (p.f.002.), isolated from the rhizosphere of date palms from the ghardaia region in the algerian sahara, to promote root growth of two varieties of maize under conditions of salt and aluminum stress. primary roots of 5-day-old seedlings were inoculated with p.f.002., and seedlings were then grown under both control and stressed conditions. primary, lateral, and seminal root lengths and numbers, as well as root ...201626759938
specific caleosin/peroxygenase and lipoxygenase activities are tissue-differentially expressed in date palm (phoenix dactylifera l.) seedlings and are further induced following exposure to the toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin.two caleosin/peroxygenase isoforms from date palm, phoenix dactylifera l., pdclo2 and pdclo4, were characterized with respect to their tissue expression, subcellular localization, and oxylipin pathway substrate specificities in developing seedlings. both pdclo2 and pdclo4 had peroxygenase activities that peaked at the mid-stage (radicle length of 2.5 cm) of seedling growth and were associated with the lipid droplet (ld) and microsomal fractions. recombinant pdclo2 and pdclo4 proteins heterologou ...201628111588
table wine from tropical fruits utilizing natural yeast isolates.an attempt was made to utilize few widely available tropical fruits to develop wine with the objective of comparing the fermentation efficiency (along with progress in fermentation) of two efficient yeast isolates with commercially available strain. fruit wine from juices of fully ripe mango, jackfruit and pineapple alone and in blended combinations of all three fruit juice (2: 1: 2) was prepared using two different yeasts (y4 and y7) isolated from natural plain date palm juice and one standard ...201627570291
enrichment of gilthead seabream (sparus aurata l.) diet with palm fruit extracts and probiotics: effects on skin mucosal immunity.fish skin mucus contains numerous immune substances still poorly studied. to date, there are no studies regarding the possible influence of dietary supplements on such important substances. in the present work, a commercial diet used as control diet was enriched with: 1) probiotic shewanella putrefaciens (pdp11 diet, 10(9) cfu g(-1)); 2) probiotic bacillus sp. (bacillus diet, 10(9) ufc g(-1)); 3) aqueous date palm fruits extracts (dpe diet, 4%), and 4) a combination of pdp11 + bacillus sp + aque ...201626712151
antileishmanial activity of date (phoenix dactylifera l) fruit and pit extracts in vitro.leishmaniasis is considered as a major public health problem worldwide. current drugs in treatment of leishmaniasis have some limitations; thus, the current study was aimed to assess the methanolic extracts of pit and fruit of phoenix dactylifera against leishmania major promastigotes. l major promastigotes were cultured in rpmi 1640 and incubated at 25°c ± 1°c for 24, 48, and 72 hours. for obtaining the ic50 (half maximal inhibitory concentration) value, mtt assay was employed. furthermore, pro ...201627242378
phytochemical evaluation, antioxidant assay, antibacterial activity and determination of cell viability (j774 and thp1 alpha cell lines) of p. sylvestris leaf crude and methanol purified fractions.phoenix sylvestris (arecaceae family) known as indian date palm has been identified as a component of traditional medicine against various ailments. the present study was focused on phytochemical screening of crude hexane, dichloromethane and methanol leaf extracts. the crude extracts showed the presence of alkaloids, flavonoids, and phenols in the plant leaves. in the study methanol extract was found most potent, so this extract was further fractionated by column chromatography and 9 methanol p ...201627047320
the antibacterial activity of date syrup polyphenols against s. aureus and e. coli.plant-derived products such as date syrup (ds) have demonstrated antibacterial activity and can inhibit bacteria through numerous different mechanisms, which may be attributed to bioactive compounds including plant-derived phenolic molecules. ds is rich in polyphenols and this study hypothesized that ds polyphenols demonstrate inherent antimicrobial activity, which cause oxidative damage. this investigation revealed that ds has a high content of total polyphenols (605 mg/100 g), and is rich in t ...201626952177
phenolic profile, antibacterial and cytotoxic properties of second grade date extract from tunisian cultivars (phoenix dactylifera l.).the present study aimed to investigate the phenolic profile of second grade date extracts and evaluate their antimicrobial and cytotoxic activities with regard to some pathogenic microorganisms. phenolic content was analyzed by hplc. antimicrobial activity was evaluated by the agar disk diffusion method, and in vitro cytotoxic activity was examined by cell proliferation assay. the results revealed that second grade dates presented three benzoic acids, five cinnamic acids and two flavonoids, with ...201626471652
molecular identification of potential denitrifying bacteria and use of d-optimal mixture experimental design for the optimization of denitrification process.three bacterial strains (te1, td3 and fb2) were isolated from date palm (degla), pistachio and barley. the presence of nitrate reductase (narg) and nitrite reductase (nirs and nirk) genes in the selected strains was detected by pcr technique. molecular identification based on 16s rdna sequencing method was applied to identify positive strains. in addition, the d-optimal mixture experimental design was used to optimize the optimal formulation of probiotic bacteria for denitrification process. str ...201626893037
identification of strain isolated from dates (phœnix dactylifera l.) for enhancing very high gravity ethanol production.ethanol production from by-products of dates in very high gravity was conducted in batch fermentation using two yeasts, saccharomyces cerevisiae and zygosaccharomyces rouxii, as well as a native strain: an osmophilic strain of bacteria which was isolated for the first time from the juice of dates (phoenix dactylifera l.). the phylogenetic analysis based on the 16s ribosomal rna and gyrb sequence and physiological analysis indicated that the strain identified belongs to the genus of bacillus, b. ...201627838909
antibacterial properties and effects of fruit chilling and extract storage on antioxidant activity, total phenolic and anthocyanin content of four date palm (phoenix dactylifera) cultivars.phoenix dactylifera or date palm fruits are reported to contain natural compounds that exhibit antioxidant and antibacterial properties. this research aimed to study the effect of fruit chilling at 4 °c for 8 weeks, extract storage at -20 °c for 5 weeks, and extraction solvents (methanol or acetone) on total phenolic content (tpc), antioxidant activity and antibacterial properties of saudi arabian p. dactylifera cv mabroom, safawi and ajwa, as well as iranian p. dactylifera cv mariami. the stora ...201627023514
draft genome sequence of endophytic bacterium enterobacter asburiae pda134, isolated from date palm (phoenix dactylifera l.) roots.in this report, a draft of the enterobacter asburiae strain pda134 genome was sequenced. this bacterial strain was isolated from the root tissue of a date palm, where it has the ability to produce 1-aminocyclopropane-1-carboxylic acid (acc) deaminase and indole-3-acetic acid (iaa) under salinity stress.201627540071
feasibility study of a cosmetic cream added with aqueous extract and oil from date (phoenix dactylifera l.) fruit seed using experimental design.this article reports on the feasibility study of a cosmetic cream added with aqueous extract and oil from date (phoenix dactylifera l.) fruit seed using experimental design. first, the mixture design was applied to optimize the cosmetic formula. the responses (dependent variables) were the spreadability (ysp) and viscosity (yvis), the factors (independent variables) being the weight proportions of the fatty phase (x1), the aqueous date seed extract (x2), and the beeswax (x3). second, the cosmeti ...201627125011
454-pyrosequencing reveals variable fungal diversity across farming systems.oasis farming system is common in some parts of the world, especially in the arabian peninsula and several african countries. in oman, the farming system in the majority of farms follows a semi-oasis farming (sof) system, which is characterized by growing multiple crops mainly for home consumption, but also for local market. this study was conducted to investigate fungal diversity using pyrosequencing approach in soils from a farm utilizing a sof system which is cultivated with date palms, acid ...201627014331
recent progress for the utilization of curcuma longa, piper nigrum and phoenix dactylifera seeds against type 2 diabetes.diabetes mellitus is an important human disease afflicting many from various walks of life in different countries. even though modern medicines contribute a variety of effective treatment options, they can have several unfavourable effects. the intention of this review is to organize and discuss various studies that have been previously conducted on the effectiveness of these herbal plants in diabetes.201627399905
impact of date palm fruits extracts and probiotic enriched diet on antioxidant status, innate immune response and immune-related gene expression of european seabass (dicentrarchus labrax).the application of additives in the diet as plants or extracts of plants as natural and innocuous compounds has potential in aquaculture as an alternative to antibiotics and immunoprophylactics. the aim of the current study was to evaluate the potential effects of dietary supplementation of date palm fruit extracts alone or in combination with pdp11 probiotic on serum antioxidant status, on the humoral and cellular innate immune status, as well as, on the expression levels of some immune-related ...201627033470
genome-wide identification and transferability of microsatellite markers between palmae species.the palmae family contains 202 genera and approximately 2800 species. except for elaeis guineensis and phoenix dactylifera, almost no genetic and genomic information is available for palmae species. therefore, this is an obstacle to the conservation and genetic assessment of palmae species, especially those that are currently endangered. the study was performed to develop a large number of microsatellite markers which can be used for genetic analysis in different palmae species. based on the ass ...201627826307
herbal medicines for leucorrhea according to iranian traditional medicine.leucorrhea or vaginal discharge is a conventional complaint. it is generally whitish, yellowish, or greenish vaginal discharge in females that might be normal or a symptom of infection. it is almost mucus discharge, which exhibit exfoliation of vaginal epithelial cells due to estrogen influence on the vaginal mucosa. it is important to identify the differences between physiologic and pathologic discharges. leucorrhea is a well-known disease in iranian traditional medicine (itm). in their manuscr ...201627840502
herbal medicines for leucorrhea according to iranian traditional medicine.leucorrhea or vaginal discharge is a conventional complaint. it is generally whitish, yellowish, or greenish vaginal discharge in females that might be normal or a symptom of infection. it is almost mucus discharge, which exhibit exfoliation of vaginal epithelial cells due to estrogen influence on the vaginal mucosa. it is important to identify the differences between physiologic and pathologic discharges. leucorrhea is a well-known disease in iranian traditional medicine (itm). in their manuscr ...201627516669
socio-economic characterisation of date palm (phoenix dactylifera l.) growers and date value chains in pakistan.increasing food production to feed its rapidly growing population is a major policy goal of pakistan. the production of traditional staples such as rice (oryza sativa l.) and bread wheat (triticum aestivum l.) has been intensified in many regions, but not in remote, drought-ridden areas. in these arid, marginal environments dates and their by-products are an option to complement staples given their high nutritive value and storability. to fill knowledge gaps about the role of date palm in the ho ...201627536506
the use of high throughput dna sequence analysis to assess the endophytic microbiome of date palm roots grown under different levels of salt stress.date palms are able to grow under diverse abiotic stress conditions including in saline soils, where microbial communities may be help in the plant's salinity tolerance. these communities able to produce specific growth promoting substances can enhance date palm growth in a saline environment. however, these communities are poorly defined. in the work reported here, the date palm endophytic bacterial and fungal communities were identified using the pyrosequencing method, and the microbial differ ...201628494084
the complete plastid genome sequence of eustrephus latifolius (asparagaceae: lomandroideae).the complete chloroplast (cp) genome sequence of eustrephus latifolius was firstly determined in subfamily lomandriodeae of family asparagaceae. it was 159,736 bp and contained a large single copy region (82,403 bp) and a small single copy region (13,607 bp) which were separated by two inverted repeat regions (31,863 bp). in total, 132 genes were identified and they were consisted of 83 coding genes, 8 rrna genes, 38 trna genes, 3 pseudogenes. rpl23 and clpp were pseudogenes due to sequence dele ...201625186113
periostitis of the metatarsal caused by a date palm thorn in a child: a case report.date palm thorn injuries are common in middle eastern countries, where there are many date palm plantations. if detected, these injuries can be treated without subsequent complications. in children, however, the diagnosis can very easily be missed. an untreated embedded thorn may cause late complications, including periostitis or osteomyelitis. in this article, the author presents a case of date palm thorn-induced periostitis of the fourth metatarsal. the lesion was successfully treated in this ...201621354009
antioxidant phenolic extracts obtained from secondary tunisian date varieties (phoenix dactylifera l.) by hydrothermal treatments.three common non-commercial tunisian date varieties were treated by two thermal systems, obtaining a liquid fraction which was characterized and its antioxidant capacity determined. the concentration of total phenols in the three varieties (smeti, garen gazel, and eguwa) was increased by steam explosion treatment up to 5311, 4680, and 3832 mg/kg of fresh dates, and their antioxidant activity up to 62.5, 46.5 and 43.1 mmol trolox/kg of fresh date, respectively. both thermal treatments increased t ...201626593573
the tunisian oasis ecosystem is a source of antagonistic bacillus spp. producing diverse antifungal lipopeptides.the use of microbial products has become a promising alternative approach to controlling plant diseases caused by phytopathogenic fungi. bacteria isolated from the date palm tree rhizosphere of the tunisian oasis ecosystem could provide new biocontrol microorganisms adapted to extreme conditions, such as drought, salinity and high temperature. the aim of this study was to screen bacteria isolated from the rhizosphere of the date palm tree for their ability to inhibit phytopathogenic fungi, and t ...201626428248
the major proteins of the seed of the fruit of the date palm (phoenix dactylifera l.): characterisation and emulsifying properties.proteins were extracted from the seeds of the fruit of the date palm. proteomic analysis and sds-page electrophoresis of the extracted proteome suggested it is composed predominantly of the storage proteins glycinin and β-conglycinin, although over 300 proteins were detected, 91 of which were identified with confidence. in terms of protein type, the largest numbers of proteins were associated, not unexpectedly, with metabolism and energy functions, which reflected the requirements of the germina ...201626617019
the role of date palm (phoenix dactylifera l) pollen in fertility: a comprehensive review of current evidence.date palm pollen (dpp) is the male reproductive dust of palm flowers used as dietary supplement especially as aphrodisiac and fertility enhancer in both women and men from ancient times. although there are few clinical trials evaluating the beneficial effects of dpp in humans, various experimental studies have been conducted on the reproductive effects of dpp. among the compounds isolated from dpp are amino acids, fatty acids, flavonoids, saponins, and estroles. the present review summarizes com ...201626438718
date palm and the activated sludge co-composting actinobacteria sanitization potential.the objective of this study was to find a connection between the development of the compost actinobacteria and the potential involvement of antagonistic thermophilic actinomycetes in compost sanitization as high temperature additional role. an abundance of actinobacteria and coliforms during the activated sludge and date palm co-composting is determined. hundred actinomycete isolates were isolated from the sample collected at different composting times. to evaluate the antagonistic effects of th ...201626102058
projected future distribution of date palm and its potential use in alleviating micronutrient deficiency.micronutrient deficiency develops when nutrient intake does not match nutritional requirements for maintaining healthy tissue and organ functions which may have long-ranging effects on health, learning ability and productivity. inadequacy of iron, zinc and vitamin a are the most important micronutrient deficiencies. consumption of a 100 g portion of date flesh from date palm (phoenix dactylifera l.) has been reported to meet approximately half the daily dietary recommended intake of these micron ...201625847224
the domestication syndrome in phoenix dactylifera seeds: toward the identification of wild date palm populations.investigating crop origins is a priority to understand the evolution of plants under domestication, develop strategies for conservation and valorization of agrobiodiversity and acquire fundamental knowledge for cultivar improvement. the date palm (phoenix dactylifera l.) belongs to the genus phoenix, which comprises 14 species morphologically very close, sometimes hardly distinguishable. it has been cultivated for millennia in the middle east and in north africa and constitutes the keystone of o ...201627010707
susceptibility and possible resistance mechanisms in the palm species phoenix dactylifera, chamaerops humilis and washingtonia filifera against rhynchophorus ferrugineus (olivier, 1790) (coleoptera: curculionidae).rhynchophorus ferrugineus, known as the red palm weevil (rpw), is reported as a pest of up to 40 palm species. however, the susceptibility degree and the defense mechanisms of these species against this weevil are still poorly known. in europe, the rpw is a major pest of phoenix canariensis while other palm species, including the congeneric phoenix dactylifera, seem to be less suitable hosts for this insect. the aim of our study was to compare the defensive response of p. dactylifera, chamaerops ...201626976073
effect of ultrafiltration process on physico-chemical, rheological, microstructure and thermal properties of syrups from male and female date palm saps.this study investigates the effect of the ultrafiltration process on physicochemical, rheological, microstructure and thermal properties of syrups from male and female date palm sap. all the studied syrups switched from pseudoplastic rheological behaviour (n=0.783) to newtonian behaviour (n∼1) from 10 to 50 °c respectively and present similar thermal profiles. results revealed that the ultrafiltration process significantly affects the rheological behaviour of the male and female syrups. these di ...201626948603
macroporous natural capsules extracted from phoenix dactylifera l. spore and their application in oral drugs delivery.macroporous natural sporopollenin exine capsules (sec) were extracted from date palm (phoenix dactylifera l.) and coated by natural polymer composite (carboxymethyl cellulose with epichlorohydrin). the polymer coated exine capsules (pcec) were used in in-vitro investigations for controlled delivery of paracetamol. sec, pcec, and drugs loaded capsules (pcec-par) were characterized by scanning electron microscope (sem), surface area (bet), fourier-transform infrared (ft-ir), x-ray diffraction (xrd ...201626945735
evaluation of pollen germination of some palm males and pollination impact on bunch weight and fruit quality in kadary date palm cultivar (phoenix dactylifera l.).the present study was carried out during two successive seasons (2013-2014) at the research and agriculture experiment station, dirab, college of food and agricultural sciences, king saud university, riyadh. pollen grains, used in this trial, were collected from eleven male, namely succary, menify, sallag, dikhiny, nabout zamel, serry, khalas, shagra, safry, maktumi and kadary in order to pollinate female kadary date palm cultivar. results indicated that pollens of safry male followed by succary ...201626930872
exogenous proline mediates alleviation of cadmium stress by promoting photosynthetic activity, water status and antioxidative enzymes activities of young date palm (phoenix dactylifera l.).the ability of exogenous compatible solutes, such as proline, to counteract cadmium (cd) inhibitory effects in young date palm plants (phoenix dactylifera l. cv deglet nour) was investigated. two-year-old date palm plants were subjected for five months at different cd stress levels (0, 10 and 30 mg cdcl2 kg(-1) soil) whether supplied or not with exogenous proline (20mm) added through the irrigation water. different levels of cd stress altered plant growth, gas exchanges and chlorophyll content a ...201626901506
endemic insular and coastal tunisian date palm genetic diversity.the breeding of crop species relies on the valorisation of ancestral or wild varieties to enrich the cultivated germplasm. the tunisian date palm genetic patrimony is being threatened by diversity loss and global climate change. we have conducted a genetic study to evaluate the potential of spontaneous coastal resources to improve the currently exploited tunisian date palm genetic pool. eighteen microsatellite loci of phoenix dactylifera l. were used to compare the genetic diversity of coastal a ...201626895027
delivery of ibuprofen by natural macroporous sporopollenin exine capsules extracted from phoenix dactylifera l.sporopollenin macroporous capsules (smcs) were extracted from date palm (phoenix dactylifera l.) spores and coated by a natural polymer composite (chitosan with glutaraldehyde). the polymer coated macroporous capsules smc@poly were used in the in vitro-controlled delivery of ibuprofen. the materials obtained were characterized through spectral, thermal, scanning electron microscopy (sem), x-ray diffraction (xrd), and nitrogen adsorption-desorption isotherms. the ibu loading and releasing was stu ...201626872877
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