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development of genomic simple sequence repeats (ssr) by enrichment libraries in date palm.development of highly informative markers such as simple sequence repeats (ssr) for cultivar identification and germplasm characterization and management is essential for date palms genetic studies. the present study documents the development of ssr markers and assesses genetic relationships of commonly grown date palm (phoenix dactylifera l.) cultivars in different geographical regions of saudi arabia. a total of 93 novel simple sequence repeat (ssr) markers were screened for their ability to d ...201728755232
analysis of expressed sequence tags (est) in date palm.expressed sequence tags (est) were generated from a normalized cdna library of the date palm sukkari cv. to understand the high-quality and better field performance of this well-known commercial cultivar. a total of 6943 high-quality ests were generated, out of them 6671 are submitted to the genbank dbest (libest_028537). the generated ests were assembled into 6362 unigenes, consisting of 494 (14.4%) contigs and 5868 (84.53%) singletons. the functional annotation shows that the majority of the e ...201728755231
date palm sex differentiation based on fluorescence in situ hybridization (fish).in situ hybridization (ish) is used to visualize defined dna sequences in cellular preparations by hybridization of complementary probe sequences. recently, the fluorescence in situ hybridization (fish) technique has become a powerful and useful tool for the direct detection of specific dna fragments in the genome. ribosomal dna genes (45s and 5s rdna) are commonly used as markers for the physical mapping of plant chromosomes to analyze genomic organization. here we describe cytological-based ma ...201728755228
development of sex-specific pcr-based markers in date palm.molecular markers are used efficiently in the development and identification of gender-specific pcr-based markers in date palm. there is mounting evidence that different marker systems vary in their mechanisms of detecting polymorphism and genome coverage. therefore, they could complement each other to generate accurate sex-specific markers in date palm. this chapter describes the uses of pcr-based molecular markers to develop and identify the gender in different date palm genotypes; these are a ...201728755227
gender identification in date palm using molecular markers.breeding of date palm is complicated because of its long life cycle and heterozygous nature. sexual propagation of date palm does not produce true-to-type plants. sex of date palms cannot be identified until the first flowering stage. molecular markers such as random amplified polymorphic dna (rapd), sequence-characterized amplified regions (scar), and simple sequence repeats (ssr) have successfully been used to identify the sex-linked loci in the plant genome and to isolate the corresponding ge ...201728755226
early sex identification in date palm by male-specific sequence-characterized amplified region (scar) markers.date palm (phoenix dactylifera l.) is a dioecious plant, and sex of the seedlings can be determined only at the time of first flowering which takes 4-5 years. female date palm plants are of economic importance as they bear the fruit. therefore, sex identification at an early stage is highly desirable. dna-based markers are useful for early sex detection. in this chapter, we describe male-specific sequence-characterized amplified region (scar) markers to identify sex in date palm at the seedling ...201728755225
molecular identification of date palm cultivars using random amplified polymorphic dna (rapd) markers.ambiguity in the total number of date palm cultivars across the world is pointing toward the necessity for an enumerative study using standard morphological and molecular markers. among molecular markers, dna markers are more suitable and ubiquitous to most applications. they are highly polymorphic in nature, frequently occurring in genomes, easy to access, and highly reproducible. various molecular markers such as restriction fragment length polymorphism (rflp), amplified fragment length polymo ...201728755224
genotyping and molecular identification of date palm cultivars using inter-simple sequence repeat (issr) markers.molecular markers are credible for the discrimination of genotypes and estimation of the extent of genetic diversity and relatedness in a set of genotypes. inter-simple sequence repeat (issr) markers rapidly reveal high polymorphic fingerprints and have been used frequently to determine the genetic diversity among date palm cultivars. this chapter describes the application of issr markers for genotyping of date palm cultivars. the application involves extraction of genomic dna from the target cu ...201728755223
determining phylogenetic relationships among date palm cultivars using random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers.investigation of genetic variation and phylogenetic relationships among date palm (phoenix dactylifera l.) cultivars is useful for their conservation and genetic improvement. various molecular markers such as restriction fragment length polymorphisms (rflps), simple sequence repeat (ssr), representational difference analysis (rda), and amplified fragment length polymorphism (aflp) have been developed to molecularly characterize date palm cultivars. pcr-based markers random amplified polymorphic ...201728755222
molecular analysis of date palm genetic diversity using random amplified polymorphic dna (rapd) and inter-simple sequence repeats (issrs).the date palm is an ancient domesticated plant with great diversity and has been cultivated in the middle east and north africa for at last 5000 years. date palm cultivars are classified based on the fruit moisture content, as dry, semidry, and soft dates. there are a number of biochemical and molecular techniques available for characterization of the date palm variation. this chapter focuses on the dna-based markers random amplified polymorphic dna (rapd) and inter-simple sequence repeats (issr ...201728755221
assessing date palm genetic diversity using different molecular markers.molecular marker technologies which rely on dna analysis provide powerful tools to assess biodiversity at different levels, i.e., among and within species. a range of different molecular marker techniques have been developed and extensively applied for detecting variability in date palm at the dna level. recently, the employment of gene-targeting molecular marker approaches to study biodiversity and genetic variations in many plant species has increased the attention of researchers interested in ...201728755220
date palm genetic diversity analysis using microsatellite polymorphism.date palm (phoenix dactylifera l.) is considered one of the great socioeconomic resources in the middle east and the arab regions. the tree has been and still is at the center of the comprehensive agricultural development. the number of known date palm cultivars, distributed worldwide, is approximately 3000. the success of genetic diversity conservation or any breeding program depends on an understanding of the amount and distribution of the genetic variation already in existence in the genetic ...201728755219
genetic diversity analysis of date palm using random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr).random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) are pcr-based molecular techniques used for evaluation and characterization of date palm to find the best genotype and male/female identification at an early stage. genetic fingerprinting using molecular markers is an important tool for the analysis of genetic diversity and cultivar identification. here, we present an improved dna extraction protocol using leaf tissue, based on the standard cetyltrimethyl ammonium br ...201728755218
molecular identification of fungal contamination in date palm tissue cultures.fungal contamination of in vitro cultures of date palm (phoenix dactylifera l.) is the major constraint to their initiation and maintenance. different molecular approaches have been applied successfully to analyze both inter- and intraspecific variation among fungal species as well as determine their identity. this chapter describes step-by-step procedures of molecular identification of fungal contaminants by internal transcribed spacer (its) products of the most common fungal contaminants of da ...201728755217
evaluation of clonal fidelity of micropropagated date palm by random amplified polymorphic dna (rapd).date palm is a fruit-bearing tree commonly found in arid and semiarid regions. it is a dioecious plant, producing fruit on female plants and a limited number of basal offshoots for propagation. to produce large numbers of uniform plantlets, tissue culture techniques are required. it is highly advisable to detect genetic variation that may occur through micropropagation techniques as it may lead to phenotypic alterations. random amplified polymorphic dna (rapd) is a simple and pcr-based molecular ...201728755216
encapsulation of date palm somatic embryos: synthetic seeds.synthetic seed or encapsulated somatic embryos may be used for propagation, storage, and exchange of plant germplasm and have many diverse applications in date palm cultivation. they have advantages over conventional use of offshoot material for germplasm propagation, maintenance, exchange, and transportation. this chapter describes a protocol for date palm synthetic seed production by encapsulation of somatic embryos with sodium alginate. among three concentrations used, 3% sodium alginate foll ...201728755215
in vitro conservation of date palm somatic embryos using growth-retardant conditions.in vitro conservation is carried out to maintain disease-free genetic materials, in a small area, protected against pests, insects, soil problems (alkaline, acidic, excess salinity, lack of organic matter, too dry, or too wet), climatic changes, and high-multiplication potential. a requirement of successful in vitro conservation is that the plants can be regenerated into complete plants rapidly when desired. the current work describes in vitro propagation and conservation techniques employing sl ...201728755214
in vitro conservation of date palm shoot-tip explants and callus cultures under minimal growth conditions.date palm fruit production has great economic significance for many countries. there is a fundamental necessity to conserve valuable date palm germplasm, but there are various problems with in vivo and ex situ conservation. in vitro storage has several advantages over conventional germplasm conservation methods. the in vitro technique offers a developed method of slow-growth storage, which is considered as an alternate solution for short- and medium-term storage of date palm germplasm under cont ...201728755213
in vitro cryopreservation of date palm caulogenic meristems.cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. in this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free ms medium. the positive impact of sucrose precul ...201728755212
cryopreservation of date palm pro-embryonic masses using the d cryo-plate technique.in this chapter, we describe a cryopreservation (liquid nitrogen, -196 °c) protocol developed for long-term storage of date palm pro-embryonic masses (pems), which uses the recently established d cryo-plate technique. clumps of pems (3-5 mm in size) were dissected from pem cultures and placed on pretreatment medium containing 171 g/l sucrose for 3 days. clumps were placed in the wells of aluminum cryo-plates in which they were made to adhere using droplets of 3% calcium alginate. pems were treat ...201728755211
in vitro conservation of date palm tissue cultures.in vitro technology offers a potential solution for the conservation of date palm germplasm. slow growth induced by low temperature allows storage from several months up to few years. otherwise, cryopreservation is suitable for long-term in vitro conservation, at between -79 and -196 °c. this chapter describes a protocol for cold storage at 5 °c and cryopreservation of date palm tissue cultures. for cold storage, 70% of shoot buds remain healthy after storing for 12 months at 5 °c, and callus cu ...201728755210
storage and viability assessment of date palm pollen.pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. here we describe pollen storage methods, such as room temperature (25-30 °c), refrigeration (4 °c), storage at 4 °c in desiccators, deep freezer (-20 °c), and cryopreservation (-196 °c). b ...201728755209
plant domestication: wild date palms illuminate a crop's sticky origins.in a new study, previously unknown populations of wild date palm have been identified in remote areas of oman. genomic analyses indicate date palm domestication occurred in the eastern portion of the arabian peninsula and reveal substantial subsequent gene flow with african palm populations.201728743015
the discovery of wild date palms in oman reveals a complex domestication history involving centers in the middle east and africa.for many crops, wild relatives constitute an extraordinary resource for cultivar improvement [1, 2] and also help to better understand the history of their domestication [3]. however, the wild ancestor species of several perennial crops have not yet been identified. perennial crops generally present a weak domestication syndrome allowing cultivated individuals to establish feral populations difficult to distinguish from truly wild populations, and there is frequently ongoing gene flow between wi ...201728712568
antioxidant system response and cdna-scot marker profiling in phoenix dactylifera l. plant under salinity stress.many phoenix dactylifera (date palm) cultivars are grown in the arid and semiarid regions of the world, including saudi arabia. p. dactylifera is highly tolerant to salinity stress. to investigate the response of khalas cultivar of p. dactylifera, two-month-old plants were treated with sodium chloride (50, 100, and 150 mm nacl) for three months. our result showed that proline content was higher in all treated plants compared to control plants. thiobarbituric acid reactive substances (tbars) were ...201728702461
the desert plant phoenix dactylifera closes stomata via nitrate-regulated slac1 anion channel.date palm phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. how are palms under such harsh environmental conditions able to limit transpirational water loss? here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from p. dactylifera. to understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast ab ...201728670699
synthetic co-attractants of the aggregation pheromone of the date palm root borer oryctes agamemnon.laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest oryctes agamemnon are reported. volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in olfactometers alone or combined with synthetic pheromone. a collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic p ...201728667548
cardiopreventive effect of ethanolic extract of date palm pollen against isoproterenol induced myocardial infarction in rats through the inhibition of the angiotensin-converting enzyme.the present study aimed to examine the putative preventive effect of the ethanolic extract date palm pollen (dpp, phoenix dactylifera l., family arecaceae) on isoproterenol-induced myocardial infarction (mi) in rats. twenty four rats were randomly divided into four groups including control. they were treated with dpp extract (400mg/kg) and clopidogrel (0.2mg/kg) for 7days followed by myocardial injury induction using subcutaneous isoproterenol (100mg/kg) with an interval of 24h for two days (6th ...201728645882
antibacterial activities of diospyros blancoi, phoenix dactylifera and morus nigra against dental caries causing pathogens: an in vitro study.the study was undertaken to determine the in-vitro antibacterial potential of diospyrosblancoi, phoenix dactylifera and morusnigra leaf extracts in hexane, chloroform, methanol, ethyl-acetate and aqueous extracts against dental caries causing bacteria. disc diffusion assay was used to determine the antibacterial efficacy; the extracts were further separated using thin layer chromatography and the anti-biofilm activity of the extracts was also determined. the preliminary phytochemical screening o ...201728603127
apparent metabolizable energy value of whole date palm (phoenix dactylifera l.) and its possible use as a feedstuff for aged laying hens.this study was conducted to evaluate the feeding value of whole date palm (wdp) with and without enzyme supplementation on aged laying hen's performance. apparent metabolizable energy value of wdp was determined by the total collection method using ten adult leghorn cockerels. wdp was substituted with a corn-soybean meal basal diet at 40% level, and then the metabolizable energy of this experimental diet and basal diet was determined. after that, a total number of 256 bovanz 95-week-old hens wer ...201728597141
detecting early signs of heat and drought stress in phoenix dactylifera (date palm).plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. here we studied the adaptation strategies in date palm, phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. in transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it ...201728570677
impact of environmental variables on dubas bug infestation rate: a case study from the sultanate of oman.date palm cultivation is economically important in the sultanate of oman, with significant financial investment coming from both the government and from private individuals. however, a global infestation of dubas bug (ommatissus lybicus bergevin) has impacted the middle east region, and infestations of date palms have been widespread. in this study, spatial analysis and geostatistical techniques were used to model the spatial distribution of dubas bug infestations to (a) identify correlations be ...201728558069
charting novel allergens from date palm pollen (phoenix sylvestris) using homology driven proteomics.pollen grains from phoenix sylvestris (date palm), a commonly cultivated tree in india has been found to cause severe allergic diseases in an increasing percentage of hypersensitive individuals. to unearth its allergenic components, pollen protein were profiled by two-dimensional gel electrophoresis followed by immunoblotting with date palm pollen sensitive patient sera. allergens were identified by maldi-tof/tof employing a layered proteomic approach combining conventional database dependent se ...201728535932
three new species of arbuscular mycorrhizal fungi discovered at one location in a desert of oman: diversispora omaniana, septoglomus nakheelum and rhizophagus arabicus.three new species of arbuscular mycorrhizal (am) fungi (glomeromycota) were isolated from soil samples collected from a hyperarid sandy plain of south arabia. morphological characteristics of the spores clearly differentiated them from closely related am species. molecular analyses were performed on rdna sequences obtained from single spores including a ~1700 bp region comprising partial ssu, its, partial lsu and the ~600 bp its region only. the phylogenetic trees based on these regions showed t ...201724782493
chemical composition of alfalfa silage with waste date and its feeding effect on ruminal fermentation characteristics and microbial protein synthesis in sheep.this study was conducted to evaluate the effect of feeding ensiled alfalfa with waste date on ruminal fermentation characteristics, microbial protein synthesis, protozoa population and blood parameters in sheep. eight rams were used in a 2 × 2 change over design. each experimental period consisted of 21 days including 16 days for adaptation and 5 days for sampling. for ensiling, fresh alfalfa (medicago sativa l.) with different levels of waste date (phoenix dactylifera l.) were mixed together an ...201727600493
development and validation of an hplc-uv method for determination of eight phenolic compounds in date palms.a simple, precise, accurate, and selective method was developed and validated for determination of eight phenolic compounds (gallic acid, p-hydroxybenzoic acid, vanilic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, and sinapic acid) in date palms. separation was achieved on an rp c18 column using the mobile phase methanol-water with 2% acetic acid (18+82, v/v). this method was validated according to the requirements for new methods, which include accuracy, precision, selectiv ...201726525252
diet rich in date palm fruits improves memory, learning and reduces beta amyloid in transgenic mouse model of alzheimer's disease.at present, the treatment options available to delay the onset or slow down the progression of alzheimer's disease (ad) are not effective. recent studies have suggested that diet and lifestyle factors may represent protective strategies to minimize the risk of developing ad. date palm fruits are a good source of dietary fiber and are rich in total phenolics and natural antioxidants, such as anthocyanins, ferulic acid, protocatechuic acid and caffeic acid. these polyphenolic compounds have been s ...201726167001
ethnobotanical survey of phoenix dactylifera l. pollen used for the treatment of infertility problems in algerian oases.the phoenix dactylifera l. (date palm) is known for its traditional medicinal properties across the history of native population in algerian sahara. there is a large trend of consumption of date palm pollen preparations in many human infertility cases in our country. however, the validity has not been scientifically tested. there has been no direct scientific research on this application. this study was undertaken to identify cultivars with greater potential in the traditional medicine uses. to ...201728480429
date stones of phoenix dactylifera and jujube shells of ziziphus lotus as potential biosorbents for anionic dye removal.this study investigated the biosorption of congo red (cr) from aqueous solution by date stones (ds) of phoenix dactylifera and jujube shells (js) of ziziphus lotus. batch operations were carried out in the liquid phase to observe the effect of various experimental parameters such as contact time, ph, temperature, and initial dye concentration on removal of cr. the characteristics of the ds and js were also examined by fourier transform infrared analysis. the biosorption data of cr on ds and js s ...201728463549
hydrothermal pretreatment and enzymatic hydrolysis of mixed green and woody lignocellulosics from arid regions.utilization of multi-specie feedstocks is imperative for application of lignocellulosic biorefineries in arid regions. different lignocellulosic residues vary in composition and anatomical features. pretreatment and enzymatic hydrolysis are two processes at the front end of any lignocellulosics biorefinery applying biochemical pathway, and have to efficiently deal with the variance in the feedstock composition and properties. however, there is limited knowledge about effect of mixing different l ...201728456045
somatic embryogenesis from bud and leaf explants of date palm (phoenix dactylifera l.) cv. najda.an efficient regeneration system through somatic embryogenesis was developed for date palm cv. najda. adventitious bud and proximal leaf segments cultured on murashige and skoog (ms) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. somatic embryogenesis induction seemed correlated with the type of the explant, the induction period and the auxin used. the highest rate of somatic embryogenesis (86.0%) was obtained on b ...201728444601
date palm waste biochars alter a soil respiration, microbial biomass carbon, and heavy metal mobility in contaminated mined soil.a 30-day incubation experiment was conducted using a heavy metal-contaminated mined soil amended with date palm feedstock (fs) and its derivative biochars (bcs) at three pyrolysis temperatures of 300 (bc-300), 500 (bc-500), and 700 °c (bc-700) with different application rates (0.0, 5, 15, and 30 g kg-1) to investigate their short-term effects on soil respiration (co2-c efflux), microbial biomass carbon (mbc), soil organic carbon (soc), mobile fraction of heavy metals (cd, cu, pb, zn, mn, and fe) ...201728424945
diversity of algerian oases date palm (phoenix dactylifera l., arecaceae): heterozygote excess and cryptic structure suggest farmer management had a major impact on diversity.date palm (phoenix dactyliferal.) is the mainstay of oasis agriculture in the saharan region. it is cultivated in a large part of the mediterranean coastal area of the sahara and in most isolated oases in the algerian desert. we sampled 10 oases in algeria to understand the structure of date palm diversity from the coastal area to a very isolated desert location. we used 18 microsatellite markers and a chloroplast minisatellite to characterize 414 individual palm trees corresponding to 114 named ...201728410422
identification and evaluation of antibacterial agents present in lipophilic fractions isolated from sub-products of phoenix dactilyfera.in this study, the dried powder of seeds and pollen of phoenix dactylifera was extracted using soxhlet extractor and biochemical composition of lipophilic extracts was analysed by gcms. then, we have tested the antibacterial effect of fatty acids and volatile compounds found in these sub-products. the results revealed that the main fatty acids found in deglet nour seeds is are oleic acid (42.06%) followed by capric acid (24.16%), takerbucht seeds extract contains mostly oleic acid (40.59%) and p ...201728403631
effect of various levels of date palm kernel on growth performance of broilers.the aim of this study was the assessment of various levels of date palm kernel (dpk) on the growth performance of broilers.201728344407
date palm waste-derived biochar composites with silica and zeolite: synthesis, characterization and implication for carbon stability and recalcitrant potential.engineered organo-mineral composites were synthesized from date palm waste biochar and silica or zeolite via mechanochemical treatments. date palm tree rachis (leaves) waste biomass was pre-treated with silica or zeolite minerals via ball milling and sonication prior to pyrolysis at 600 °c. the resultant organo-mineral composites and pristine materials were characterized using x-ray diffraction, thermogravimetric-differential thermal (tg-dta), fourier transform infrared, scanning electron micros ...201728337620
genome-wide expression profiling in leaves and roots of date palm (phoenix dactylifera l.) exposed to salinity.date palm, as one of the most important fruit crops in north african and west asian countries including oman, is facing serious growth problems due to salinity, arising from persistent use of saline water for irrigation. although date palm is a relatively salt-tolerant plant species, its adaptive mechanisms to salt stress are largely unknown.201728330456
toxicity and detoxification mechanism of black pepper and its major constituent in controlling rhynchophorus ferrugineus olivier (curculionidae: coleoptera).the survival, feeding response, and detoxification mechanism of rhynchophorus ferrugineus olivier, a key pest responsible for destruction of date palm, was examined with different extracts of piper nigrum and its major constituent (piperine) identified by gc-ms. in the present study, toxicity of different extracts of black pepper was evaluated by incorporating diffferent doses of extracts into the artificial diet of red palm weevil larvae. all extracts showed dose-dependent insecticidal activity ...201728326461
prevalence of skin sensitization to pollen of date palm in marrakesh, morocco.background. date palm's pollen has been identified as a source of allergy; the rate of sensitization of this pollen is between 6 and 29%. objective. to determine the prevalence of sensitization to date palm in marrakesh and to identify the clinical profile. patients and methods. this study is based on a questionnaire and the prick test on 7 allergens, in population aged 5 years and above with clinical symptoms suggesting allergic diseases, from november 2012 to february 2013 in marrakech. result ...201728270850
tentative characterization of polyphenolic compounds in the male flowers of phoenix dactylifera by liquid chromatography coupled with mass spectrometry and dft.phoenix dacylifera is an ancient palm species rich in (poly)phenols. these phenolic compounds were tentatively identified by using liquid chromatography coupled with ion spray mass spectrometry in tandem mode (lc/ms/ms) with negative ion detection. negative identification of the compounds was based on their retention times and mass spectra in full scan mode (ms), and in different ms/ms modes. for the first time, complete hypothesis, and routs for both p-coumaroylshikimic acids (cosa) and caffeoy ...201728257091
endophytic fungi associated with roots of date palm (phoenix dactylifera) in coastal dunes.symbiotic interactions with fungal endophytes are argued to be responsible for the tolerance of plants to some stresses and for their adaptation to natural conditions.201728254367
short-term effects of date palm extract (phoenix dactylifera) on ischemia/reperfusion injury induced by testicular torsion/detorsion in rats.antioxidants are potent scavengers of free radicals and have beneficial effects on human health.201728250657
the phytochelatin synthase gene in date palm (phoenix dactylifera l.): phylogeny, evolution and expression.we studied date palm phytochelatin synthase type i (pdpcs1), which catalyzes the cytosolic synthesis of phytochelatins (pcs), a heavy metal binding protein, in plant cells. the gene encoding pdpcs1 (pdpcs) consists of 8 exons and 7 introns and encodes a protein of 528 amino acids. pcs gene history was studied using notung phylogeny. during evolution, gene loss from several lineages was predicted including proteobacteria, bilateria and brassicaceae. in addition, eleven gene duplication events app ...201728231507
batch and fixed bed adsorption of levofloxacin on granular activated carbon from date (phoenix dactylifera l.) stones by koh chemical activation.granular activated carbon (kac) was prepared from abundant phoenix dactylifera l. stones by microwave- assisted koh activation. the characteristics of kac were tested by pore analyses, scanning electron microscopy (sem) and fourier transforms infrared spectroscopy (ftir). the adsorption behavior of levofloxacin (lev) antibiotic on kac with surface area of 817m2/g and pore volume of 0.638cm3/g were analyzed using batch and fixed bed systems. the equilibrium data collected by batch experiments wer ...201728189062
extraction optimization and in vitro and in vivo anti-postprandial hyperglycemia effects of inhibitor from phoenix dactylifera l. parthenocarpic fruit.phoenix dactylifera l. plays an important role in social, economic, and ecological tunisian sectors. some date palms produce parthenocarpic fruit named sish. the aqueous ethanolic extract from p. dactylifera parthenocarpic dates demonstrated a potent inhibition of the enzymes related to type ii diabetes. in this work, extraction optimization of amylase inhibitors was carried out using box-behnken design. bioactivity-guided fractionation of the 70% aqueous ethanol extract was performed to identif ...201728167450
zinc oxide nanoparticles affect carbon and nitrogen mineralization of phoenix dactylifera leaf litter in a sandy soil.we investigated the impact of zinc oxide nanoparticles (zno nps; 1000mgkg-1 soil) on soil microbes and their associated soil functions such as date palm (phoenix dactylifera) leaf litter (5gkg-1 soil) carbon and nitrogen mineralization in mesocosms containing sandy soil. nanoparticles application in litter-amended soil significantly decreased the cultivable heterotrophic bacterial and fungal colony forming units (cfu) compared to only litter-amended soil. the decrease in cfu could be related to ...201727810328
transfer factors for natural radioactivity into date palm pits.palm pits are used in various human and animal feed products. in this study, the natural radioactivity levels from soil and date palm pits of 9 samples collected from major date palm farms in three different regions (buraidah, al-zulfi and al-majmaah) of saudi arabia were determined by using the high purity germanium (hpge) gamma-ray spectrometer. the mean activity concentrations of 226ra, 232th, 137cs and 40k in soil samples were 12.8 ± 2.2, 10.2 ± 2.1, 0.28 ± 0.10 and 329 ± 87 bg kg-1, respect ...201727894743
evaluation of the efficacy of insecticidal coatings based on teflutrin and chlorpyrifos against rhynchophorus ferrugineus.the date palm (phoenix dactylifera l.), an important economic resource for many nations worldwide, has recently been threatened by the presence of different insect pests, like the red palm weevil (rpw) rhynchophorus ferrugineus.201728094903
fingerprinting of strong spermatogenesis steroidal saponins in male flowers of phoenix dactylifera (date palm) by lc-esi-ms.a fingerprint of steroid saponins, the major constituent in 80% methanolic fraction from the male flowers of phoenix dactylifera has been established. under esi-ms/ms conditions, the fragmentation patterns of [m - h]- ions exclusively displayed signals corresponding to the cleavage of the glycosidic bonds, thus allowing a rapid identification of 21 steroidal saponins. moreover, two unique among them conjugated with histidine were detected by lc-esi (-)-ms and dft and were given tentative names o ...201728049352
adsorption of ciprofloxacin and norfloxacin from aqueous solution onto granular activated carbon in fixed bed column.carbonization of phoenix dactylifera l stones followed by microwave k2co3 activation was adopted for preparation of granular activated carbon (kac). high yield and favorable pore characteristics in terms of surface area and pore volume were reported for kac as follows: 44%, 852m2/g, and 0.671cm3/g, respectively. the application of kac as adsorbent for attraction of ciprofloxacin (cip) and norfloxacin (nor) was investigated using fixed bed systems. the effect of flow rate (0.5-1.5ml/min), bed hei ...201728040619
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
the genome draft of coconut (cocos nucifera).coconut palm (cocos nucifera,2n = 32), a member of genus cocos and family arecaceae (palmaceae), is an important tropical fruit and oil crop. currently, coconut palm is cultivated in 93 countries, including central and south america, east and west africa, southeast asia and the pacific islands, with a total growth area of more than 12 million hectares [1]. coconut palm is generally classified into 2 main categories: "tall" (flowering 8-10 years after planting) and "dwarf" (flowering 4-6 years af ...201729048487
codon usage and codon pair patterns in non-grass monocot genomes.studies on codon usage in monocots have focused on grasses, and observed patterns of this taxon were generalized to all monocot species. here, non-grass monocot species were analysed to investigate the differences between grass and non-grass monocots.201729155926
effect of increasing levels of wasted date palm on digestion, rumen fermentation and microbial protein synthesis in sheep.this study was conducted to evaluate the effects of increasing levels of wasted date (wd) in sheep (initial body weight 50 ± 2.3 kg) diets on apparent diet digestibility, rumen fermentation and microbial protein synthesis. the experimental diets contained 0 (0 wd), 9% (9 wd), 18% (18 wd) or 27% (27 wd) of wasted dates. these diets were evaluated through a 4 × 4 latin square design with four 28-day periods. sheep were individually housed in metabolic cages and were fed diets ad libitum. to keep t ...201727039841
metabolome based volatiles profiling in 13 date palm fruit varieties from egypt via spme gc-ms and chemometrics.dates (phoenix dactylifera l.) are distributed worldwide as major food complement providing a source of sugars and dietary fiber as well as macro- and micronutrients. although phytochemical analyses of date fruit non-volatile metabolites have been reported, much less is known about the aroma given off by the fruit, which is critical for dissecting sensory properties and quality traits. volatile constituents from 13 date varieties grown in egypt were profiled using spme-gcms coupled to multivaria ...201727664623
removal of zn2+ and so42- from aqueous solutions on acidic and chelating dehydrated carbon.the agricultural waste, date palm leaflets, was carbonized chemically using sulfuric acid treatment. produced dehydrated carbon (dc) was subjected to surface functionalization using ethylene diamine producing chelating dehydrated carbon (cdc). in the process, ∼80 % of the carboxylic content on dc was converted to amide successfully. dc acts as a cation exchanger because of the high content of carboxylic groups on its surface showing acidic nature. however, cdc possesses amine and amide groups sh ...201727155837
characterization of two minicircular plasmid-like dnas isolated from date-palm mitochondria.we report here the identification and characterization of two minicircular plasmid-like dnas isolated from mitochondria of a moroccan date-palm variety. both molecules were cloned and used as probes in southern analyses of mitochondrial and total-cellular dna. evidence was obtained that these plasmid-like dnas cross-hybridized but did not show any homology to nuclear, chloroplastic, or main mitochondrial genomes. sequence analysis revealed that both minicircles, 1,346- and 1,160-bp long, share s ...20177874749
transcriptome sequencing and de novo assembly in arecanut, areca catechu l elucidates the secondary metabolite pathway genes.areca catechu l. belongs to the arecaceae family which comprises many economically important palms. the palm is a source of alkaloids and carotenoids. the lack of ample genetic information in public databases has been a constraint for the genetic improvement of arecanut. to gain molecular insight into the palm, high throughput rna sequencing and de novo assembly of arecanut leaf transcriptome was undertaken in the present study. a total 56,321,907 paired end reads of 101 bp length consisting of ...201829321980
the effect of hydro-alcoholic of phoenix dactylifera extract on sleep and eeg in rat.hard envelope of date palm pollen is used as a sedative and calmative compounds in iranian traditional medicine. we tried to study the effects of phoenix dactylifera (tarooneh) extract on sleep time and brian waves.201829299434
an extract from date palm fruit (phoenix dactylifera) acts as a co-agonist ligand for the nuclear receptor fxr and differentially modulates fxr target-gene expression in vitro.date palm fruit (phoenix dactylifera) consumption reduces serum triglyceride levels in human subjects. the objective of this study was to prepare an extract from dates and determine whether it acts as a ligand for the farnesoid x receptor (fxr), a nuclear receptor important for maintaining triglyceride and cholesterol homeostasis. freeze-dried extracts were isolated from california-grown dates (deglet noor and medjool) from the 2014 and 2015 harvests, by means of liquid extraction and solid phas ...201829293579
differential dna methylation and transcription profiles in date palm roots exposed to salinity.as a salt-adaptive plant, the date palm (phoenix dactylifera l.) requires a suitable mechanism to adapt to the stress of saline soils. there is growing evidence that dna methylation plays an important role in regulating gene expression in response to abiotic stresses, including salinity. thus, the present study sought to examine the differential methylation status that occurs in the date palm genome when plants are exposed to salinity, and to identify salinity responsive genes that are regulated ...201829352281
occurrence of ochratoxins, fumonisin b2 , aflatoxins (b1 and b2 ), and other secondary fungal metabolites in dried date palm fruits from egypt: a mini-survey.this study was conducted to investigate the natural co-occurrence of 295 fungal and bacterial metabolites in 28 samples of dried date palm fruits collected from different shops distributed in assiut governorate, upper egypt in 2016. extraction and quantification of the target analytes were done using the "dilute and shoot" approach followed by liquid chromatography-tandem mass spectrometry (lc-ms/ms) analysis. in total, 30 toxic fungal metabolites were detected. among these metabolites, 4 types ...201829350762
pyrolysis of date palm waste in a fixed-bed reactor: characterization of pyrolytic products.the pyrolysis of several tunisian date palm wastes (dpw): date palm rachis (dpr), date palm leaflets (dpl), empty fruit bunches (efb) and date palm glaich (dpg) was run using a fixed-bed reactor, from room temperature to 500°c, with 15°c/min as heating rate and -5°c as condensation temperature, in order to produce bio-oil, biochar and syngas. in these conditions, the bio-oil yield ranges from 17.03wt% for dpl to 25.99wt% for efb. for the biochar, the highest yield (36.66wt%) was obtained for dpl ...201828954249
phosphorus-loaded biochar changes soil heavy metals availability and uptake potential of maize (zea mays l.) plants.biochar (bc) was produced by pyrolyzing the date palm leaf waste at 600 °c and then loaded with phosphorus (p) via sorption process. greenhouse pot experiment was conducted to investigate the application effects of bc and p-loaded biochar (bcp) on growth and availability of p and heavy metals to maize (zea mays l.) plants grown in contaminated mining soil. the treatments consisted of bc and bcp (at application rates of 5, 10, 20, and 30 g kg-1 of soil), recommended nk and npk, and a control (no ...201829220749
effect of pollen, pit powder, and gemmule extract of date palm on male infertility: a systematic review.pollen, pit powder, and gemmule extract of the date palm are rich in flavonoids. antioxidant characteristics of the flavonoids have a significant effect on prevention and treatment of infertility. the aim of the present study was to review studies investigating the effects of pollen, pit powder, and gemmule extract of the date palm on male infertility. articles that were published about the topic between 2005 and 2016 were reviewed from different databases in turkish and english. the search engi ...201829087245
production and structural characterization of exopolysaccharides from newly isolated probiotic lactic acid bacteria.in this work, four exopolysaccharide-producing lactic acid bacteria (lab) strains, newly isolated from tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (bms and tms), date palm sap (dps) and cow milk (cm), were identified as leuconostoc citreum-bms, leuconostoc mesenteroides-tms, pediococcus pentosaceus-dps and leuconostoc pseudomesenteroides-cm, respectively. the isolated strains showed the ability to withstand simulated human gastrointestinal (gi) tr ...201829080817
ameliorative effects of bee pollen and date palm pollen on the glycemic state and male sexual dysfunctions in streptozotocin-induced diabetic wistar rats.this study aimed to assess the effects of bee pollen (bp) and/or date palm pollen (dpp) suspensions on the glycemic state, testicular dysfunctions, oxidative stress and antioxidant defense system in streptozotocin (stz)-induced diabetic male wistar rats. diabetes mellitus was induced by single intraperitoneal injection of stz to overnight-fasted rats at dose of 40mg/kg body weight. after 1 week of stz injection, diabetic rats were treated with bp and/or dpp suspensions at dose levels of 100mg/kg ...201829080463
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