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microrna expression in multistage date fruit development.micrornas (mirnas) are small endogenous noncoding rnas. plant mirnas are known to play important regulatory roles in homeostasis, stress response, and diverse developmental processes. here, we describe the identification of conserved mirnas in date palm (phoenix dactylifera l.) based on transcriptomic data acquired across multistage fruit development and genome sequences, which include 238 plant conserved mirnas and 276 novel p. dactylifera-specific mirnas.201728755233
development of genomic simple sequence repeats (ssr) by enrichment libraries in date palm.development of highly informative markers such as simple sequence repeats (ssr) for cultivar identification and germplasm characterization and management is essential for date palms genetic studies. the present study documents the development of ssr markers and assesses genetic relationships of commonly grown date palm (phoenix dactylifera l.) cultivars in different geographical regions of saudi arabia. a total of 93 novel simple sequence repeat (ssr) markers were screened for their ability to d ...201728755232
naa-induced direct organogenesis from female immature inflorescence explants of date palm.micropropagation has great potential for the multiplication of female and male date palms of commercially grown cultivars by using inflorescences. this approach is simple, convenient, and much faster than the conventional method of using shoot-tip explants. we describe here a stepwise micropropagation procedure using inflorescence explants of iraqi date palm cultivar maktoom. cultured explants were derived from 0.5-cm-long spike segments excised from 8 to 10-cm-long spathes. about 70% formed adv ...201728755332
analysis of expressed sequence tags (est) in date palm.expressed sequence tags (est) were generated from a normalized cdna library of the date palm sukkari cv. to understand the high-quality and better field performance of this well-known commercial cultivar. a total of 6943 high-quality ests were generated, out of them 6671 are submitted to the genbank dbest (libest_028537). the generated ests were assembled into 6362 unigenes, consisting of 494 (14.4%) contigs and 5868 (84.53%) singletons. the functional annotation shows that the majority of the e ...201728755231
date palm sex differentiation based on fluorescence in situ hybridization (fish).in situ hybridization (ish) is used to visualize defined dna sequences in cellular preparations by hybridization of complementary probe sequences. recently, the fluorescence in situ hybridization (fish) technique has become a powerful and useful tool for the direct detection of specific dna fragments in the genome. ribosomal dna genes (45s and 5s rdna) are commonly used as markers for the physical mapping of plant chromosomes to analyze genomic organization. here we describe cytological-based ma ...201728755228
cultivar-dependent direct organogenesis of date palm from shoot tip explants.a number of public and private laboratories are working on date palm micropropagation to meet the increasing worldwide demand for date palm planting material. a standardized direct organogenesis protocol exists for the production of date palm plantlets to maintain the genetic fidelity of regenerated plants. organogenesis has the advantage of using low concentrations of plant growth regulators and avoiding the callus phase. in addition, direct regeneration of vegetative buds minimizes the risk of ...201728755331
date fruit proteomics during development and ripening stages.gel-based comparative proteomics approach is a valuable technique for studying the changes in abundance of proteins in any given system. the combination of this technique with mass spectrometry has provided immense insight into protein dynamics during fruit development and ripening. this chapter describes, informatively, the procedures for carrying out comparative proteomics analysis of date palm (phoenix dactylifera l.) fruits at different developmental stages using a combination of two-dimensi ...201728755236
development of sex-specific pcr-based markers in date palm.molecular markers are used efficiently in the development and identification of gender-specific pcr-based markers in date palm. there is mounting evidence that different marker systems vary in their mechanisms of detecting polymorphism and genome coverage. therefore, they could complement each other to generate accurate sex-specific markers in date palm. this chapter describes the uses of pcr-based molecular markers to develop and identify the gender in different date palm genotypes; these are a ...201728755227
gender identification in date palm using molecular markers.breeding of date palm is complicated because of its long life cycle and heterozygous nature. sexual propagation of date palm does not produce true-to-type plants. sex of date palms cannot be identified until the first flowering stage. molecular markers such as random amplified polymorphic dna (rapd), sequence-characterized amplified regions (scar), and simple sequence repeats (ssr) have successfully been used to identify the sex-linked loci in the plant genome and to isolate the corresponding ge ...201728755226
plant regeneration from somatic embryogenic suspension cultures of date palm.somatic embryogenesis is one of the most important technologies for plant regeneration of elite date palm cultivars. recently, considerable progress has been made in the development and optimization of this technique from embryogenic cell suspension cultures. this chapter describes a procedure for the rapid development of a large number of somatic embryos from embryogenic cell suspension cultures. an efficient plant regeneration protocol via somatic embryogenesis from cell suspension cultures st ...201728755347
improvement of in vitro date palm plantlet acclimatization rate with kinetin and hoagland solution.in vitro propagation of date palm phoenix dactylifera l. is an ideal method to produce large numbers of healthy plants with specific characteristics and has the ability to transfer plantlets to ex vitro conditions at low cost and with a high survival rate. this chapter describes optimized acclimatization procedures for in vitro date palm plantlets. primarily, the protocol presents the use of kinetin and hoagland solution to enhance the growth of barhee cv. plantlets in the greenhouse at two stag ...201728755346
controlling hyperhydricity in date palm in vitro culture by reduced concentration of nitrate nutrients.hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropagation. several factors have been ascribed as being responsible for hyperhydricity, which are related to the explant, medium, culture vessel, and environment. the optimization of inorganic nutrients in the culture medium improves in vitro growth and morphogenesis, in addition to controlling hyperhydricity. this chapter describes a protocol for controlling hyperhydricity during the embryogenic callus ...201728755345
histological analysis of the developmental stages of direct somatic embryogenesis induced from in vitro leaf explants of date palm.somatic embryogenesis is an ideal technique for the micropropagation of date palm using different explant tissue; however, histological studies describing the ontogenesis of plant regeneration are limited. this chapter provides a simple protocol for the histological analysis of the successive developmental stages of direct somatic embryogenesis induced from in vitro leaf explants. direct somatic embryos are obtained from murashige and skoog (ms) medium containing 2 mg/l 6-benzylaminopurine. in o ...201728755343
histological evidence of indirect somatic embryogenesis from immature female date palm inflorescences.rapid production of somatic embryogenesis and date palm regeneration is achieved by culturing immature female inflorescence explants. inflorescence explants are soft, creamy in color, average 6-7 cm in length, and cultured on murashige and skoog (ms) medium containing 1 mg/l thidiazuron (tdz). callus induction occurs after 4-5 weeks of culture on the callus induction medium. subsequently, callus develops embryogenic calli on ms medium supplemented with 0.1 mg/l naphthalene acetic acid (naa). his ...201728755342
desiccation and cold hardening of date palm somatic embryos improve germination.embryogenic suspension cultures of date palm are ideal for mass propagation of somatic embryos; however, the low percentage of germination of somatic embryos (se) remains an impediment. this chapter focuses on two important physical factors to improve germination of date palm somatic embryos: the use of partial desiccation (3 h) of somatic embryos and the exposure to low temperature (4 °c for 24 h). high germination percentage (41%) is achieved by desiccation for 3 h. moreover, adding 0.3 g/l ac ...201728755341
desiccation-enhanced maturation and germination of date palm somatic embryos derived from cell suspension culture.in vitro plant regeneration via somatic embryogenesis is a powerful tool for rapid, large-scale production of healthy true-to-type plants. this approach is suitable to preserve existing natural genetic variability and propagation of variability generated from genetic improvement programs, including crossing, somaclonal variation, mutagenesis, and somatic hybridization. this chapter outlines a simplified protocol for date palm regeneration via somatic embryogenesis induced in cell suspension cult ...201728755340
indirect somatic embryogenesis of date palm using juvenile leaf explants and low 2,4-d concentration.this chapter describes an efficient protocol for large-scale micropropagation of date palm. somatic embryo-derived plants are regenerated from highly proliferating suspension cultures. friable embryogenic callus is initiated from juvenile leaves using slightly modified murashige and skoog (ms) medium supplemented with 0.1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-d). suspension cultures consisting of proembryonic masses are established from highly competent callus for somatic embryogenesis using ...201728755339
indirect somatic embryogenesis from mature inflorescence explants of date palm.due to the limitations associated with shoot tip explants in the micropropagation of date palm, inflorescence explants are an ideal alternative. this chapter focuses on the protocol for the induction of callus from inflorescence tissue, establishment for proliferation of somatic embryos, germination, elongation, rooting, and acclimatization. female inflorescences, 30-40 cm in length, cv. shaishee, were used for culture initiation. after disinfection, the outer inflorescence cover (spathe) is cut ...201728755338
enhanced indirect somatic embryogenesis from shoot-tip explants of date palm by gradual reductions of 2,4-d concentration.shoot-tip explants obtained from offshoots of adult date palms are an excellent source for callus induction and subsequent somatic embryogenesis. in this protocol, the shoot-tip explants are transferred sequentially to a series of media containing gradually reduced concentrations of plant growth hormones: (a) 10 mg/l 2,4-dichlorophenoxy acetic acid (2,4-d) and 3 mg/l 2-isopentenyl adenine (2ip), (b) 7 mg/l 2,4-d and 1 mg/l 2ip, (c) 5 mg/l 2,4-d and 1 mg/l 2ip, and (d) 3 mg/l 2,4-d and 1 mg/l 2ip ...201728755337
enhanced indirect somatic embryogenesis of date palm using low levels of seawater.date palm tolerates salinity, drought, and high temperatures. arid and semiarid zones, especially the middle east region, need a huge number of date palms for cultivation. to meet this demand, tissue culture techniques have great potential for mass production of plantlets, especially using the indirect embryogenesis technique; any improvement of these techniques is a worthy objective. low levels of salinity can enhance growth and development of tolerant plants. a low level of seawater, a natural ...201728755336
direct organogenesis and indirect somatic embryogenesis by in vitro reversion of mature female floral buds to a vegetative state.this protocol describes in vitro plant regeneration from mature female inflorescence explants of date palm (phoenix dactylifera l.) by reversion of floral state (reproductive phase) to the vegetative state. the mature female inflorescence (fully developed) is cultured on ms induction medium containing 10 mg/l 2,4-dichlorophenoxyacetic acid (2,4-d), 3 mg/l 2-isopentenyladenine (2ip), and 2 mg/l paclobutrazol (pbz) or 2 mg/l abscisic acid (aba). the basal part of the petals has meristematic cells, ...201728755335
optimized direct organogenesis from shoot tip explants of date palm.in vitro propagation is an available alternative to produce uniform and good-quality planting material to establish large-scale date palm cultivation in a short time. this study was carried out to achieve organogenesis and multiplication directly from shoot tips without callus formation, thus avoiding any possibility of undesirable genetic variability among the regenerated plants. the shoot tips explants are cultured on murashige and skoog (ms) medium supplemented with 1 mg/l naphthaleneacetic a ...201728755334
direct organogenesis from immature female inflorescence of date palm by gradual reduction of 2,4-d concentration.inflorescences represent an alternative explant source for superior date palm trees, especially those that do not produce offshoots. they provide large numbers of explants free of fungal and bacterial contamination for successful tissue culture initiation. furthermore, they are characterized by the capacity of plant regeneration within a short time as compared to other explant types. this chapter focuses on the procedures employed for plant regeneration by direct organogenesis using immature fem ...201728755333
early sex identification in date palm by male-specific sequence-characterized amplified region (scar) markers.date palm (phoenix dactylifera l.) is a dioecious plant, and sex of the seedlings can be determined only at the time of first flowering which takes 4-5 years. female date palm plants are of economic importance as they bear the fruit. therefore, sex identification at an early stage is highly desirable. dna-based markers are useful for early sex detection. in this chapter, we describe male-specific sequence-characterized amplified region (scar) markers to identify sex in date palm at the seedling ...201728755225
molecular identification of date palm cultivars using random amplified polymorphic dna (rapd) markers.ambiguity in the total number of date palm cultivars across the world is pointing toward the necessity for an enumerative study using standard morphological and molecular markers. among molecular markers, dna markers are more suitable and ubiquitous to most applications. they are highly polymorphic in nature, frequently occurring in genomes, easy to access, and highly reproducible. various molecular markers such as restriction fragment length polymorphism (rflp), amplified fragment length polymo ...201728755224
genotyping and molecular identification of date palm cultivars using inter-simple sequence repeat (issr) markers.molecular markers are credible for the discrimination of genotypes and estimation of the extent of genetic diversity and relatedness in a set of genotypes. inter-simple sequence repeat (issr) markers rapidly reveal high polymorphic fingerprints and have been used frequently to determine the genetic diversity among date palm cultivars. this chapter describes the application of issr markers for genotyping of date palm cultivars. the application involves extraction of genomic dna from the target cu ...201728755223
detecting early signs of heat and drought stress in phoenix dactylifera (date palm).plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. here we studied the adaptation strategies in date palm, phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. in transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it ...201728570677
report - screening of the anti-hyperglycemic activity of some medicinal plants of jordan.diabetes represents a group of common diseases that are characterized by dysregulation of blood glucose levels. plants are traditionally used for management of diseases including diabetes. in this study, we screened the anti-diabetic effect of extracts of 21 plants grown in jordan. extracts of plants were screened for their antihyperglycemic activity. diabetes was induced in sprague dawley rats using alloxan. plant extracts were dosed at 1gm/kg. blood glucose was measured at baseline and at ever ...201728653938
a vlp-based vaccine provides complete protection against nipah virus challenge following multiple-dose or single-dose vaccination schedules in a hamster model.nipah virus is a highly lethal zoonotic paramyxovirus that was first recognized in malaysia during an outbreak in 1998. during this outbreak, nipah virus infection caused a severe febrile neurological disease in humans who worked in close contact with infected pigs. the case fatality rate in humans was approximately 40%. since 2001, niv has re-emerged in bangladesh and india where fruit bats (pteropus spp.) have been identified as the principal reservoir of the virus. transmission to humans is c ...201729263876
physico-chemical characteristics and fungal profile of four saudi fresh date (phoenix dactylifera l.) cultivars.physico-chemical and microbial analyses of four commercial fresh date cultivars (sukari, barhi, khalas and rothana) grown in saudi arabia were evaluated. colorimetric assay indicated that sukari had the highest total sugar content of 13.21g/100gfw while the lowest was in rothana with 7.96g/100gfw. total phenolic content (tpc) ranged from 76.74 to 122.20mggae/100gfw in barhi and rothana respectively, whereas antioxidant activity was highest in sukari (105.99μggae/gfw) and lowest in khalas (90.81μ ...201727979253
in silico approach for characterization and comparison of repeats in the genomes of oil and date palms.transposable elements (tes) are mobile genetic elements present in almost all eukaryotic genomes. due to their typical patterns of repetition, discovery, and characterization, they demand analysis by various bioinformatics software. probably, as a result of the need for a complex analysis, many genomes publicly available do not have these elements annotated yet. in this study, a de novo and homology-based identification of tes and microsatellites was performed using genomic data from 3 palm spec ...201728469420
the effect of increasing levels of date palm (phoenix dactylifera l.) seed on the performance, ruminal fermentation, antioxidant status and milk fatty acid profile of saanen dairy goats.this study was conducted to examine the effect of date palm (phoenix dactylifera l.) seed (dps) on the performance, ruminal fermentation, antioxidant status and milk fatty acid (fa) profile of dairy goats. eight multiparous saanen dairy goats, averaging 97 ± 7 days in milk (dim) and 2150 ± 130 g of milk production, were used in a 4 × 4 replicated latin square design. experimental diets contained 0% (control), 6% (dps6), 12% (dps12) and 18% (dps18) of dps. dry matter intake (dmi), milk production ...201728045194
correction to: date palm waste-derived biochar composites with silica and zeolite: synthesis, characterization and implication for carbon stability and recalcitrant potential.unfortunately, in the original publication of the article, prof. yong sik ok's affiliation was incorrectly published.201729282621
correction to: date palm waste biochars alter a soil respiration, microbial biomass carbon, and heavy metal mobility in contaminated mined soil.unfortunately, in the original publication of the article, prof. yang sik ok's affiliation was incorrectly published. the author's affiliation is as follows.201729282620
insight into the expression variation of metal-responsive genes in the seedling of date palm (phoenix dactylifera).phytochelatin synthase and metallothionein gene expressions were monitored via qpcr in order to investigate the molecular mechanisms involved in cd and cr detoxification in date palm (phoenix dactylifera). a specific reference gene validation procedure using bestkeeper, normfinder and genorm programs allowed selection of the three most stable reference genes in a context of cd or cr contamination among six reference gene candidates, namely elongation factor α1, actin, aldehyde dehydrogenase, san ...201729334652
characterization and amplification of gene-based simple sequence repeat (ssr) markers in date palm.the paucity of molecular markers limits the application of genetic and genomic research in date palm (phoenix dactylifera l.). availability of expressed sequence tag (est) sequences in date palm may provide a good resource for developing gene-based markers. this study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (ssrs) from the est sequences in date palm. the est sequences studied are mainly homologous to those of elaeis guineensis and musa a ...201728755229
codon usage and codon pair patterns in non-grass monocot genomes.studies on codon usage in monocots have focused on grasses, and observed patterns of this taxon were generalized to all monocot species. here, non-grass monocot species were analysed to investigate the differences between grass and non-grass monocots.201729155926
process simulation and economic assessment of hydrothermal pretreatment and enzymatic hydrolysis of multi-feedstock lignocellulose - separate vs combined processing.biorefinery based on multi-feedstock lignocellulose can be viable where a sustainable supply of a single substrate is limited, for example in arid regions. processing of mixed feedstocks has been studied in lab scale, however, its economics are less studied. in this study, an economic comparison was made between separate and combined (mixed) processing approaches for multi-feedstock lignocellulose for the production of monomeric sugars. this modular approach of focusing on sugar platform makes t ...201729136939
the genome draft of coconut (cocos nucifera).coconut palm (cocos nucifera,2n = 32), a member of genus cocos and family arecaceae (palmaceae), is an important tropical fruit and oil crop. currently, coconut palm is cultivated in 93 countries, including central and south america, east and west africa, southeast asia and the pacific islands, with a total growth area of more than 12 million hectares [1]. coconut palm is generally classified into 2 main categories: "tall" (flowering 8-10 years after planting) and "dwarf" (flowering 4-6 years af ...201729048487
evaluating the nutritional value of date pits and demonstrating their application in laying hen diets.this experiment was carried out to study the feeding value of ground date pits (dp) (phoenix dactylifera l.) with and without enzyme supplementation on laying hens' performance. apparent metabolizable energy value of dp was determined by the total collection method using 10 adult leghorn cockerels. after that, a total number of 144 lohmann 50-week-old lsl-lite hens were randomly allocated into six groups consisting of four replicates of six birds, based on a 3 × 2 factorial arrangement of the tr ...201729143376
physicochemical and sensory characteristics of spreadable liver pâtés with annatto extract (bixa orellana l.) and date palm co-products (phoenix dactylifera l.).two novel ingredients were incorporated into spreadable liver pâtés to study their effect on physicochemical and sensory characteristics and their possible use in the meat industry. fresh date (phoenix dactylifera, cv. confitera) co-products, as a paste (0, 2.5 and 7.5%), and annatto (bixa orellana) extract (0 and 128 mg/kg), as a colourant, and their combinations were incorporated into liver pâtés to study their effect on the final quality. the six formulations were analysed for chemical compos ...201729109376
effect of aqueous extract of tops of date palm leaves on blood glucose of diabetic rats.present study was carried out to examine the effect of tops of date palm leaves extract on blood glucose of streptozotocin induced diabetic rats. forty male sprague dawely rats (120-130g) were housed individually and randomly allocated to two main groups; diabetic group (n=30), and normal group (n=10) in the animal lab, faculty of agriculture and veterinary medicine, qassim university, saudi arabia. an aqueous extracts were prepared from tops of date palm leaves (edpl) and were orally administer ...201729105640
genome sequencing of microbacterium sp. yaish 1, a bacterial strain isolated from the rhizosphere of date palm trees affected by salinity.microbacterium sp. strain yaish 1 is a rhizospheric bacterium isolated from date palm orchards with high soil salinity. the genome was sequenced, and genes coding for growth-promoting 1-aminocyclopropane-1-carboxylate (acc) deaminase, siderophore-producing proteins, and tryptophan biosynthesis proteins were identified. here, we report the draft whole-genome sequencing of the strain.201729097476
adsorption studies on the removal of cod and bod from treated sewage using activated carbon prepared from date palm waste.in this work, the adsorption of chemical oxygen demand (cod) and biological oxygen demand (bod) from treated sewage with low-cost activated carbon prepared from date palm shell waste by chemical activation method was studied. different parameters affecting the adsorption process such as carbon dose, ph, contact time, agitation rate, and temperature were studied. adsorption equilibrium was attained after 150 min at ph 6.0 with agitation rate of 400 rpm at 25 °c. the results showed that cod remova ...201728799143
report - antidiabetic effects of native date fruit aseel (phoenix dactylifera l.) in normal and hyperglycemic rats.change in dietary pattern, sedentary life style and increasing stresses are contributing factors for high prevalence of diabetes mellitus. diabetic complications often lead to cardiovascular diseases, hypertension and hyperlipidemia that are the leading cause death and disability all over the world. apart from pharmacotherapy, use of antihyperglycemic medicinal food is a new aspect in diabetes management and prevention of its complications. phoenix dactylifera (date palm) has been traditionally ...201729084704
variation in bacterial endosymbionts associated with the date palm hopper, ommatissus lybicus populations.the date palm hopper, ommatissus lybicus, is a key pest of the date palm, which is expected to be comprised of many allopatric populations. the current study was carried out to determine bacterial endosymbiont diversity in the different populations of this pest. ten date palm hopper populations were collected from the main date palm growing regions in iran and an additional four samples from pakistan, oman, egypt and tunisia for detection of primary and secondary endosymbionts using polymerase c ...201728807085
drdb: an online date palm genomic resource database.background: date palm (phoenix dactylifera l.) is a cultivated woody plant with agricultural and economic importance in many countries around the world. with the advantages of next generation sequencing technologies, genome sequences for many date palm cultivars have been released recently. short sequence repeat (ssr) and single nucleotide polymorphism (snp) can be identified from these genomic data, and have been proven to be very useful biomarkers in plant genome analysis and breeding. results ...201729209336
biochemical and genetical responses of phoenix dactylifera l. to cadmium stress.the cadmium (cd), a heavy metal, causes toxicity, which leads to hampering the growth and development of the plant. the molecular and biochemical approaches were used for the investigation of antioxidant system response and genotoxicity in date palm (phoenix dactylifera l.) cv. sagai in pot experiment having cd. the root length was more affected than the shoot length as more accumulation of cd occurs in roots. fresh weights of root and shoot were reduced significantly in treated plants as compar ...201729201916
abrogation of carbon tetrachloride-induced hepatotoxicity in sprague-dawley rats by ajwa date fruit extract through ameliorating oxidative stress and apoptosis.ajwa, a variety of date palme phoenix dactylifera l., has long been used and considered as one of the most popular fruits in the north africa and middle east region. for muslims this fruit is of religious importance and is mentioned several times in quran. besides being a part of the arabian essential diet, dates have been used traditionally for number of complications. this study aimed to evaluate the possible potential of ajwa date extract to guard against carbon tetrachloride (ccl4)-induced l ...201729175788
a controlled trial to reduce the risk of human nipah virus exposure in bangladesh.human nipah virus (niv) infection, often fatal in bangladesh, is primarily transmitted by drinking raw date palm sap contaminated by pteropus bats. we assessed the impact of a behavior change communication intervention on reducing consumption of potentially niv-contaminated raw sap. during the 2012-2014 sap harvesting seasons, we implemented interventions in two areas and compared results with a control area. in one area, we disseminated a "do not drink raw sap" message and, in the other area, e ...201728905152
factors affecting seawater-based pretreatment of lignocellulosic date palm residues.seawater-based pretreatment of lignocellulosic biomass is an innovative process at research stage. with respect to process optimization, factors affecting seawater-based pretreatment of lignocellulosic date palm residues were studied for the first time in this paper. pretreatment temperature (180°c-210°c), salinity of seawater (0ppt-50ppt), and catalysts (h2so4, na2co3, and naoh) were investigated. the results showed that pretreatment temperature exerted the largest influence on seawater-based p ...201728898854
highly decorated lignins in leaf tissues of the canary island date palm phoenix canariensis.the cell walls of leaf base tissues of the canary island date palm (phoenix canariensis) contain lignins with the most complex compositions described to date. the lignin composition varies by tissue region and is derived from traditional monolignols (ml) along with an unprecedented range of ml conjugates: ml-acetate, ml-benzoate, ml-p-hydroxybenzoate, ml-vanillate, ml-p-coumarate, and ml-ferulate. the specific functions of such complex lignin compositions are unknown. however, the distribution o ...201728894022
date palm biochar-polymer composites: an investigation of electrical, mechanical, thermal and rheological characteristics.the application of biochar (bc) as a filler in polymers can be viewed as a sustainable approach that incorporates pyrolysed waste based value-added material and simultaneously mitigate bio-waste in a smart way. the overarching aim of this work was to investigate the electrical, mechanical, thermal and rheological properties of biocomposite developed by utilizing date palm waste-derived bc for the reinforcing of polypropylene (pp) matrix. date palm waste derived bc prepared at (700 and 900°c) wer ...201729154049
linking pollen quality and performance of neoseiulus californicus (acari: phytoseiidae) in two-spotted spider mite management programmes.it has been shown that pollen as a dietary supplement may increase the establishment of generalist predatory mites, and therefore pest control by these mites can be provided. life table studies were performed to evaluate the nutritional value of seven different pollens (almond, castor-bean, date-palm, maize, bitter-orange, sunflower and mixed bee pollen) as a supplementary food source for the spider mite predator neoseiulus californicus mcgregor. in addition, the nutritional quality of each poll ...201727117203
fungal root microbiome from healthy and brittle leaf diseased date palm trees (phoenix dactylifera l.) reveals a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites.in this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (bld) date palm trees (phoenix dactylifera l.) widely cultivated in arid zones of tunisia. a total of 52 endophytic fungi were isolated from healthy and bld roots of date palm trees, identified based on internal transcribed spacer-rdna sequence analysis and shown to represent 13 species belonging to fi ...201728293229
transient gus gene expression in date palm fruit using agroinjection transformation technique.transient expression of foreign genes in plant tissue is a valuable tool for testing the efficacy of transformation methods. in this work, we present, for the first time, the utilization of agroinjection as an efficient transformation system for gene delivery in date palm fruit. the research utilized agrobacterium tumefaciens strain lba4404 harboring the binary vector pri201-an-gus carrying the beta-glucuronidase (gus) gene, under the control of a camv 35s and kanamycin (nptii) as an antibiotic ...201728755354
draft genome sequence of the endophytic bacillus aryabhattai strain squ-r12, identified from phoenix dactylifera l. roots.bacillus aryabhattai strain squ-r12 was isolated from date palm seedlings, where it showed a growth-promoting capacity by being able to synthesize indole-3-acetic acid phytohormone and reduce ethylene biosynthesis by producing 1-aminocyclopropane-1-carboxylic acid deaminase. the draft genome sequence of this strain is reported here.201728798173
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
mitochondrial molecular markers for resistance to bayoud disease in date palm.bayoud disease, caused by fusarium oxysporum f. sp. albedinis, is a very serious and destructive disease to date palm. screening of date palm germplasm for resistance to bayoud disease is a crucial step to avoid or alleviate the disease consequences. fortunately, it was discovered that there are two mitochondrial plasmid-like dna molecules associated with susceptibility or resistance to bayoud disease. in this chapter, we present a fast, simple, and reliable technique to screen date palm germpla ...201728755230
convergence of humans, bats, trees, and culture in nipah virus transmission, bangladesh.preventing emergence of new zoonotic viruses depends on understanding determinants for human risk. nipah virus (niv) is a lethal zoonotic pathogen that has spilled over from bats into human populations, with limited person-to-person transmission. we examined ecologic and human behavioral drivers of geographic variation for risk of niv infection in bangladesh. we visited 60 villages during 2011-2013 where cases of infection with niv were identified and 147 control villages. we compared case villa ...201728820130
a large-scale behavior change intervention to prevent nipah transmission in bangladesh: components and costs.nipah virus infection (niv) is a bat-borne zoonosis transmitted to humans through consumption of niv-contaminated raw date palm sap in bangladesh. the objective of this analysis was to measure the cost of an niv prevention intervention and estimate the cost of scaling it up to districts where spillover had been identified.201728651646
nipah virus outbreaks in bangladesh: a deadly infectious disease.during 2001-2011, multidisciplinary teams from the institute of epidemiology, disease control and research (iedcr) and international centre for diarrhoeal disease research, bangladesh(icddr,b) identified sporadic cases and 11 outbreaks of nipah encephalitis. three outbreaks were detected through sentinel surveillance; others were identified through event-based surveillance. a total of 196 cases of nipah encephalitis, in outbreaks, clusters and as isolated cases were detected from 20 districts of ...201728612796
identifying and controlling contamination of date palm tissue cultures.fungal and bacterial contaminations are major problems facing in vitro date palm (phoenix dactylifera l.) proliferation. to overcome this problem, we must first identify the fungal (e.g., alternaria sp., aspergillus niger, penicillium sp.) and bacterial (e.g., pseudomonas sp.) spread in date palm in vitro cultures. incorporating fungicides (e.g., copper oxychloride, vitavax t, and topsin m) or antibiotics (e.g., streptomycin, banocin, and bencid d) at 500 mg/l in medium significantly reduces the ...201728755344
enzymatic saccharification and fermentation of cellulosic date palm wastes to glucose and lactic acid.the bioconversion of cellulosic wastes into high-value bio-products by saccharification and fermentation processes is an important step that can reduce the environmental pollution caused by agricultural wastes. in this study, enzymatic saccharification of treated and untreated date palm cellulosic wastes by the cellulases from geobacillus stearothermophilus was optimized. the alkaline pre-treatment of the date palm wastes was found to be effective in increasing the saccharification percentage. t ...201726887233
application of optimized large surface area date stone (phoenix dactylifera ) activated carbon for rhodamin b removal from aqueous solution: box-behnken design approach.box-behnken model of response surface methodology was used to study the effect of adsorption process parameters for rhodamine b (rhb) removal from aqueous solution through optimized large surface area date stone activated carbon. the set experiments with three input parameters such as time (10-600min), adsorbent dosage (0.5-10g/l) and temperature (25-50°c) were considered for statistical significance. the adequate relation was found between the input variables and response (removal percentage of ...201728167440
illumina miseq sequencing analysis of fungal diversity in stored dates.date palm has been a major fruit tree in the middle east over thousands of years, especially in the arabian peninsula. dates are consumed fresh (rutab) or after partial drying and storage (tamar) during off-season. the aim of the study was to provide in-depth analysis of fungal communities associated with the skin (outer part) and mesocarp (inner fleshy part) of stored dates (tamar) of two cultivars (khenizi and burny) through the use of illumina miseq sequencing.201728347268
characterization of two minicircular plasmid-like dnas isolated from date-palm mitochondria.we report here the identification and characterization of two minicircular plasmid-like dnas isolated from mitochondria of a moroccan date-palm variety. both molecules were cloned and used as probes in southern analyses of mitochondrial and total-cellular dna. evidence was obtained that these plasmid-like dnas cross-hybridized but did not show any homology to nuclear, chloroplastic, or main mitochondrial genomes. sequence analysis revealed that both minicircles, 1,346- and 1,160-bp long, share s ...20177874749
effect of pollen, pit powder, and gemmule extract of date palm on male infertility: a systematic review.pollen, pit powder, and gemmule extract of the date palm are rich in flavonoids. antioxidant characteristics of the flavonoids have a significant effect on prevention and treatment of infertility. the aim of the present study was to review studies investigating the effects of pollen, pit powder, and gemmule extract of the date palm on male infertility. articles that were published about the topic between 2005 and 2016 were reviewed from different databases in turkish and english. the search engi ...201829087245
differential dna methylation and transcription profiles in date palm roots exposed to salinity.as a salt-adaptive plant, the date palm (phoenix dactylifera l.) requires a suitable mechanism to adapt to the stress of saline soils. there is growing evidence that dna methylation plays an important role in regulating gene expression in response to abiotic stresses, including salinity. thus, the present study sought to examine the differential methylation status that occurs in the date palm genome when plants are exposed to salinity, and to identify salinity responsive genes that are regulated ...201829352281
occurrence of ochratoxins, fumonisin b2 , aflatoxins (b1 and b2 ), and other secondary fungal metabolites in dried date palm fruits from egypt: a mini-survey.this study was conducted to investigate the natural co-occurrence of 295 fungal and bacterial metabolites in 28 samples of dried date palm fruits collected from different shops distributed in assiut governorate, upper egypt in 2016. extraction and quantification of the target analytes were done using the "dilute and shoot" approach followed by liquid chromatography-tandem mass spectrometry (lc-ms/ms) analysis. in total, 30 toxic fungal metabolites were detected. among these metabolites, 4 types ...201829350762
transcriptome sequencing and de novo assembly in arecanut, areca catechu l elucidates the secondary metabolite pathway genes.areca catechu l. belongs to the arecaceae family which comprises many economically important palms. the palm is a source of alkaloids and carotenoids. the lack of ample genetic information in public databases has been a constraint for the genetic improvement of arecanut. to gain molecular insight into the palm, high throughput rna sequencing and de novo assembly of arecanut leaf transcriptome was undertaken in the present study. a total 56,321,907 paired end reads of 101 bp length consisting of ...201829321980
the effect of hydro-alcoholic of phoenix dactylifera extract on sleep and eeg in rat.hard envelope of date palm pollen is used as a sedative and calmative compounds in iranian traditional medicine. we tried to study the effects of phoenix dactylifera (tarooneh) extract on sleep time and brian waves.201829299434
an extract from date palm fruit (phoenix dactylifera) acts as a co-agonist ligand for the nuclear receptor fxr and differentially modulates fxr target-gene expression in vitro.date palm fruit (phoenix dactylifera) consumption reduces serum triglyceride levels in human subjects. the objective of this study was to prepare an extract from dates and determine whether it acts as a ligand for the farnesoid x receptor (fxr), a nuclear receptor important for maintaining triglyceride and cholesterol homeostasis. freeze-dried extracts were isolated from california-grown dates (deglet noor and medjool) from the 2014 and 2015 harvests, by means of liquid extraction and solid phas ...201829293579
phosphorus-loaded biochar changes soil heavy metals availability and uptake potential of maize (zea mays l.) plants.biochar (bc) was produced by pyrolyzing the date palm leaf waste at 600 °c and then loaded with phosphorus (p) via sorption process. greenhouse pot experiment was conducted to investigate the application effects of bc and p-loaded biochar (bcp) on growth and availability of p and heavy metals to maize (zea mays l.) plants grown in contaminated mining soil. the treatments consisted of bc and bcp (at application rates of 5, 10, 20, and 30 g kg-1 of soil), recommended nk and npk, and a control (no ...201829220749
production and structural characterization of exopolysaccharides from newly isolated probiotic lactic acid bacteria.in this work, four exopolysaccharide-producing lactic acid bacteria (lab) strains, newly isolated from tunisian spontaneously fermented foods and beverages, namely bovine and turkey meat sausages (bms and tms), date palm sap (dps) and cow milk (cm), were identified as leuconostoc citreum-bms, leuconostoc mesenteroides-tms, pediococcus pentosaceus-dps and leuconostoc pseudomesenteroides-cm, respectively. the isolated strains showed the ability to withstand simulated human gastrointestinal (gi) tr ...201829080817
ameliorative effects of bee pollen and date palm pollen on the glycemic state and male sexual dysfunctions in streptozotocin-induced diabetic wistar rats.this study aimed to assess the effects of bee pollen (bp) and/or date palm pollen (dpp) suspensions on the glycemic state, testicular dysfunctions, oxidative stress and antioxidant defense system in streptozotocin (stz)-induced diabetic male wistar rats. diabetes mellitus was induced by single intraperitoneal injection of stz to overnight-fasted rats at dose of 40mg/kg body weight. after 1 week of stz injection, diabetic rats were treated with bp and/or dpp suspensions at dose levels of 100mg/kg ...201829080463
pyrolysis of date palm waste in a fixed-bed reactor: characterization of pyrolytic products.the pyrolysis of several tunisian date palm wastes (dpw): date palm rachis (dpr), date palm leaflets (dpl), empty fruit bunches (efb) and date palm glaich (dpg) was run using a fixed-bed reactor, from room temperature to 500°c, with 15°c/min as heating rate and -5°c as condensation temperature, in order to produce bio-oil, biochar and syngas. in these conditions, the bio-oil yield ranges from 17.03wt% for dpl to 25.99wt% for efb. for the biochar, the highest yield (36.66wt%) was obtained for dpl ...201828954249
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