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mitochondrial dna variation among populations of rhynchophorus ferrugineus (coleoptera: curculionidae) from pakistan.the red palm weevil (rpw) rhynchophorus ferrugineus (olivier) is a voracious pest of palm species. in recent decades its range has expanded greatly, particularly impacting the date palm industry in the middle east. this has led to conjecture regarding the origins of invasive rpw populations. for example, in parts of the middle east, rpw is commonly referred to as the "pakistani weevil" in the belief that it originated there. we sought evidence to support or refute this belief. first reports of r ...201627651423
metabolome based volatiles profiling in 13 date palm fruit varieties from egypt via spme gc-ms and chemometrics.dates (phoenix dactylifera l.) are distributed worldwide as major food complement providing a source of sugars and dietary fiber as well as macro- and micronutrients. although phytochemical analyses of date fruit non-volatile metabolites have been reported, much less is known about the aroma given off by the fruit, which is critical for dissecting sensory properties and quality traits. volatile constituents from 13 date varieties grown in egypt were profiled using spme-gcms coupled to multivaria ...201727664623
evaluation of anti-diabetic and anti-tumoral activities of bioactive compounds from phoenix dactylifera l's leaf: in vitro and in vivo approach.among various chronic disorders, cancer and diabetes mellitus are the most common disorders. this study was designed to evaluate the effectiveness of hydroalcoholic extract of phoenix dactylifera l. leaves (hepdl) in animal models of type ii diabetes in vitro/in vivo and in a human melanoma-derived cell line (igr-39). a liquid chromatography-tandem mass spectrometry (lc-ms/ms) analysis was also performed to determine the amount of phenolic and flavonoid compounds in this plant. the physicochemic ...201627668542
analysis of natural and anthropogenic radionuclide content in palm date fruit of the united arab emirates: a baseline study.the purpose of this study is to contribute to a wider effort of establishing an environmental radiation baseline for the united arab emirates (uae) before the startup of the country's first nuclear reactor in 2017. an investigation of gamma-emitting radionuclide concentrations in palm dates grown in the uae was performed. palm date samples of 10 varieties originating from several local commercial date palm farms of the uae were collected and analyzed. the study targeted the naturally occurring r ...201627682905
phenol-rich compounds sweet gel: a statistically more effective antibiotic than cloxacillin against pseudomonas aeruginosa.the purpose of this study was to obtain a natural antibiotic from phenol-rich compounds; for the dressing and the treatment of chronic wounds.201627695634
chemical characterisation and the anti-inflammatory, anti-angiogenic and antibacterial properties of date fruit (phoenix dactylifera l.).date fruit, phoenix dactylifera l. has traditionally been used as a medicine in many cultures for the treatment of a range of ailments such as stomach and intestinal disorders, fever, oedema, bronchitis and wound healing.201627729284
investigating rare risk factors for nipah virus in bangladesh: 2001-2012.human nipah encephalitis outbreaks have been identified almost yearly in bangladesh since 2001. though raw date palm sap consumption and person-to-person contact are recognized as major transmission pathways, alternative pathways of transmission are plausible and may not have been identified due to limited statistical power in each outbreak. we conducted a risk factor analysis using all 157 cases and 632 controls surveyed in previous investigations during 2004-2012 to identify exposures independ ...201627738775
comparative study of neuropharmacological, analgesic properties and phenolic profile of ajwah, safawy and sukkari cultivars of date palm (phoenix dactylifera).in addition to the rich nutritional value, date palm is also used in various ethnobotanical practices for the treatment of various disease conditions. present investigation was undertaken to examine the neuropharmacological and antinociceptive effect of the ethanol extract of three date cultivars growing in saudi arabia, namely ajwah, safawy and sukkari. neuropharmacological effect was observed by pentobarbitone induced sleeping time, open field, and hole board test. antinociceptive activity was ...201627746708
date fruits-assisted synthesis and biocompatibility assessment of nickel oxide nanoparticles anchored onto graphene sheets for biomedical applications.nanographene- and graphene-based nanohybrids have garnered attention in the biomedical community owing to their biocompatibility, excellent aqueous processability, ease of cellular uptake, facile surface functionalization, and thermal and electrical conductivities. nio nanoparticle-graphene nanohybrid (g-nio) was synthesized by first depositing ni(oh)2 onto the surface of graphene oxide (go) sheets. the ni(oh)2-go hybrids were then reduced to g-nio using date palm syrup at 85 °c. the prepared g- ...027778152
discrimination and nitric oxide inhibitory activity correlation of ajwa dates from different grades and origin.this study was aimed at examining the variations in the metabolite constituents of the different ajwa grades and farm origins. it is also targeted at establishing the correlations between the metabolite contents and the grades and further to the nitric oxide (no) inhibitory activity. identification of the metabolites was generated using ¹h-nmr spectroscopy metabolomics analyses utilizing multivariate methods. the no inhibitory activity was determined using a griess assay. multivariate data analy ...201627801841
zinc oxide nanoparticles affect carbon and nitrogen mineralization of phoenix dactylifera leaf litter in a sandy soil.we investigated the impact of zinc oxide nanoparticles (zno nps; 1000mgkg-1 soil) on soil microbes and their associated soil functions such as date palm (phoenix dactylifera) leaf litter (5gkg-1 soil) carbon and nitrogen mineralization in mesocosms containing sandy soil. nanoparticles application in litter-amended soil significantly decreased the cultivable heterotrophic bacterial and fungal colony forming units (cfu) compared to only litter-amended soil. the decrease in cfu could be related to ...201727810328
identification of reference genes for quantitative real-time pcr in date palm (phoenix dactylifera l.) subjected to drought and salinity.date palm is an important crop plant in the arid and semi-arid regions supporting human population in the middle east and north africa. these areas have been largely affected by drought and salinity due to insufficient rainfall and improper irrigation practices. date palm is a relatively salt- and drought-tolerant plant and more recently efforts have been directed to identifying genes and pathways that confer stress tolerance in this species. quantitative real-time pcr (qpcr) is a promising tech ...201627824922
genome-wide identification and transferability of microsatellite markers between palmae species.the palmae family contains 202 genera and approximately 2800 species. except for elaeis guineensis and phoenix dactylifera, almost no genetic and genomic information is available for palmae species. therefore, this is an obstacle to the conservation and genetic assessment of palmae species, especially those that are currently endangered. the study was performed to develop a large number of microsatellite markers which can be used for genetic analysis in different palmae species. based on the ass ...201627826307
identification of strain isolated from dates (phœnix dactylifera l.) for enhancing very high gravity ethanol production.ethanol production from by-products of dates in very high gravity was conducted in batch fermentation using two yeasts, saccharomyces cerevisiae and zygosaccharomyces rouxii, as well as a native strain: an osmophilic strain of bacteria which was isolated for the first time from the juice of dates (phoenix dactylifera l.). the phylogenetic analysis based on the 16s ribosomal rna and gyrb sequence and physiological analysis indicated that the strain identified belongs to the genus of bacillus, b. ...201627838909
herbal medicines for leucorrhea according to iranian traditional medicine.leucorrhea or vaginal discharge is a conventional complaint. it is generally whitish, yellowish, or greenish vaginal discharge in females that might be normal or a symptom of infection. it is almost mucus discharge, which exhibit exfoliation of vaginal epithelial cells due to estrogen influence on the vaginal mucosa. it is important to identify the differences between physiologic and pathologic discharges. leucorrhea is a well-known disease in iranian traditional medicine (itm). in their manuscr ...201627840502
proteome analysis of date palm (phoenix dactylifera l.) under severe drought and salt stress.date palm cultivars differently tolerate salinity and drought stress. this study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. eighteen-month-old date palm plants were subjected to severe salt (48 g/l nacl) and drought (82.5 g/l peg or no irrigation) conditions for one month. using a protein 2d electrophoresis method, 55 protein spots were analyzed using mass spectrometry. atp synthase cf1 alpha chains were significantly upregu ...201627840818
physico-chemical properties of biodiesel manufactured from waste frying oil using domestic adsorbents.we have evaluated the efficiency of sugar cane bagasse ash (scba), date palm seed carbon (dpsc), and rice husk ash (rha) as natural adsorbents and compared them with the synthetic adsorbent magnesol xl for improving the quality of waste frying oil (wfo) and for the impact on the physicochemical properties of the obtained biodiesel. we measured moisture content, refractive index (ri), density, acid value (av), iodine value (iv), peroxide value (pv), and saponification value (sv), as well as fatty ...201527877789
transfer factors for natural radioactivity into date palm pits.palm pits are used in various human and animal feed products. in this study, the natural radioactivity levels from soil and date palm pits of 9 samples collected from major date palm farms in three different regions (buraidah, al-zulfi and al-majmaah) of saudi arabia were determined by using the high purity germanium (hpge) gamma-ray spectrometer. the mean activity concentrations of 226ra, 232th, 137cs and 40k in soil samples were 12.8 ± 2.2, 10.2 ± 2.1, 0.28 ± 0.10 and 329 ± 87 bg kg-1, respect ...201727894743
phoenix dactylifera mediated green synthesis of cu2o particles for arsenite uptake from water.in this study, an environmentally friendly, cost-effective, and single-step procedure is used for the synthesis of polycrystalline cu2o particles with controlled morphologies. simple sugars are extracted from date fruit pulp (phoenix dactylifera) and used as a reducing agent for the formation of cu2o particles in aqueous medium. the feasibility of this solution is compared with the standard glucose solution. the cu2o particles are characterized by electron microscopy, x-ray diffraction, optical ...201627933116
physico-chemical characteristics and fungal profile of four saudi fresh date (phoenix dactylifera l.) cultivars.physico-chemical and microbial analyses of four commercial fresh date cultivars (sukari, barhi, khalas and rothana) grown in saudi arabia were evaluated. colorimetric assay indicated that sukari had the highest total sugar content of 13.21g/100gfw while the lowest was in rothana with 7.96g/100gfw. total phenolic content (tpc) ranged from 76.74 to 122.20mggae/100gfw in barhi and rothana respectively, whereas antioxidant activity was highest in sukari (105.99μggae/gfw) and lowest in khalas (90.81μ ...201727979253
adsorption of ciprofloxacin and norfloxacin from aqueous solution onto granular activated carbon in fixed bed column.carbonization of phoenix dactylifera l stones followed by microwave k2co3 activation was adopted for preparation of granular activated carbon (kac). high yield and favorable pore characteristics in terms of surface area and pore volume were reported for kac as follows: 44%, 852m2/g, and 0.671cm3/g, respectively. the application of kac as adsorbent for attraction of ciprofloxacin (cip) and norfloxacin (nor) was investigated using fixed bed systems. the effect of flow rate (0.5-1.5ml/min), bed hei ...201728040619
the effect of increasing levels of date palm (phoenix dactylifera l.) seed on the performance, ruminal fermentation, antioxidant status and milk fatty acid profile of saanen dairy goats.this study was conducted to examine the effect of date palm (phoenix dactylifera l.) seed (dps) on the performance, ruminal fermentation, antioxidant status and milk fatty acid (fa) profile of dairy goats. eight multiparous saanen dairy goats, averaging 97 ± 7 days in milk (dim) and 2150 ± 130 g of milk production, were used in a 4 × 4 replicated latin square design. experimental diets contained 0% (control), 6% (dps6), 12% (dps12) and 18% (dps18) of dps. dry matter intake (dmi), milk production ...201728045194
fingerprinting of strong spermatogenesis steroidal saponins in male flowers of phoenix dactylifera (date palm) by lc-esi-ms.a fingerprint of steroid saponins, the major constituent in 80% methanolic fraction from the male flowers of phoenix dactylifera has been established. under esi-ms/ms conditions, the fragmentation patterns of [m - h]- ions exclusively displayed signals corresponding to the cleavage of the glycosidic bonds, thus allowing a rapid identification of 21 steroidal saponins. moreover, two unique among them conjugated with histidine were detected by lc-esi (-)-ms and dft and were given tentative names o ...201728049352
extraction, partial purification and characterization of amylase from parthenocarpic date (phoenix dactylifera): effect on cake quality.phoenix dactylifera l. plays an important role in social, economic and ecological tunisian sectors. some date palms produce parthenocarpic fruit named sish. the objective of the present study was to extract biomolecules from parthenocarpic fruit by producing value-added products from the fruits.028070892
evaluation of the efficacy of insecticidal coatings based on teflutrin and chlorpyrifos against rhynchophorus ferrugineus.the date palm (phoenix dactylifera l.), an important economic resource for many nations worldwide, has recently been threatened by the presence of different insect pests, like the red palm weevil (rpw) rhynchophorus ferrugineus.201728094903
specific caleosin/peroxygenase and lipoxygenase activities are tissue-differentially expressed in date palm (phoenix dactylifera l.) seedlings and are further induced following exposure to the toxin 2,3,7,8-tetrachlorodibenzo-p-dioxin.two caleosin/peroxygenase isoforms from date palm, phoenix dactylifera l., pdclo2 and pdclo4, were characterized with respect to their tissue expression, subcellular localization, and oxylipin pathway substrate specificities in developing seedlings. both pdclo2 and pdclo4 had peroxygenase activities that peaked at the mid-stage (radicle length of 2.5 cm) of seedling growth and were associated with the lipid droplet (ld) and microsomal fractions. recombinant pdclo2 and pdclo4 proteins heterologou ...201628111588
dietary supplementation of phoenix dactylifera seeds enhances performance, immune response, and antioxidant status in broilers.the date palm (phoenix dactylifera) seeds were utilized in some traditional medical remedies and have been investigated for their possible health benefits. this proposed study wanted to assess the effect of date palm seeds (dps) dietary supplementation in comparison to mannan-oligosaccharides (bio-mos®) and β-glucan over antioxidant and immunity events that have effect on growth and carcass performances of broilers. an aggregate of 180, one-day-old, chicks were raised in the wire-floored cages a ...201628127417
impacts of human-related practices on ommatissus lybicus infestations of date palm in oman.date palm cultivation is economically important in the sultanate of oman, with significant financial investments coming from both the government and private individuals. however, a widespread dubas bug (db) (ommatissus lybicus bergevin) infestation has impacted regions including the middle east, north africa, southeast russia, and spain, resulting in widespread damages to date palms. in this study, techniques in spatial statistics including ordinary least squares (ols), geographically weighted r ...028166300
application of optimized large surface area date stone (phoenix dactylifera ) activated carbon for rhodamin b removal from aqueous solution: box-behnken design approach.box-behnken model of response surface methodology was used to study the effect of adsorption process parameters for rhodamine b (rhb) removal from aqueous solution through optimized large surface area date stone activated carbon. the set experiments with three input parameters such as time (10-600min), adsorbent dosage (0.5-10g/l) and temperature (25-50°c) were considered for statistical significance. the adequate relation was found between the input variables and response (removal percentage of ...201728167440
extraction optimization and in vitro and in vivo anti-postprandial hyperglycemia effects of inhibitor from phoenix dactylifera l. parthenocarpic fruit.phoenix dactylifera l. plays an important role in social, economic, and ecological tunisian sectors. some date palms produce parthenocarpic fruit named sish. the aqueous ethanolic extract from p. dactylifera parthenocarpic dates demonstrated a potent inhibition of the enzymes related to type ii diabetes. in this work, extraction optimization of amylase inhibitors was carried out using box-behnken design. bioactivity-guided fractionation of the 70% aqueous ethanol extract was performed to identif ...201728167450
batch and fixed bed adsorption of levofloxacin on granular activated carbon from date (phoenix dactylifera l.) stones by koh chemical activation.granular activated carbon (kac) was prepared from abundant phoenix dactylifera l. stones by microwave- assisted koh activation. the characteristics of kac were tested by pore analyses, scanning electron microscopy (sem) and fourier transforms infrared spectroscopy (ftir). the adsorption behavior of levofloxacin (lev) antibiotic on kac with surface area of 817m2/g and pore volume of 0.638cm3/g were analyzed using batch and fixed bed systems. the equilibrium data collected by batch experiments wer ...201728189062
the phytochelatin synthase gene in date palm (phoenix dactylifera l.): phylogeny, evolution and expression.we studied date palm phytochelatin synthase type i (pdpcs1), which catalyzes the cytosolic synthesis of phytochelatins (pcs), a heavy metal binding protein, in plant cells. the gene encoding pdpcs1 (pdpcs) consists of 8 exons and 7 introns and encodes a protein of 528 amino acids. pcs gene history was studied using notung phylogeny. during evolution, gene loss from several lineages was predicted including proteobacteria, bilateria and brassicaceae. in addition, eleven gene duplication events app ...201728231507
short-term effects of date palm extract (phoenix dactylifera) on ischemia/reperfusion injury induced by testicular torsion/detorsion in rats.antioxidants are potent scavengers of free radicals and have beneficial effects on human health.201728250657
endophytic fungi associated with roots of date palm (phoenix dactylifera) in coastal dunes.symbiotic interactions with fungal endophytes are argued to be responsible for the tolerance of plants to some stresses and for their adaptation to natural conditions.201728254367
tentative characterization of polyphenolic compounds in the male flowers of phoenix dactylifera by liquid chromatography coupled with mass spectrometry and dft.phoenix dacylifera is an ancient palm species rich in (poly)phenols. these phenolic compounds were tentatively identified by using liquid chromatography coupled with ion spray mass spectrometry in tandem mode (lc/ms/ms) with negative ion detection. negative identification of the compounds was based on their retention times and mass spectra in full scan mode (ms), and in different ms/ms modes. for the first time, complete hypothesis, and routs for both p-coumaroylshikimic acids (cosa) and caffeoy ...201728257091
prevalence of skin sensitization to pollen of date palm in marrakesh, morocco.background. date palm's pollen has been identified as a source of allergy; the rate of sensitization of this pollen is between 6 and 29%. objective. to determine the prevalence of sensitization to date palm in marrakesh and to identify the clinical profile. patients and methods. this study is based on a questionnaire and the prick test on 7 allergens, in population aged 5 years and above with clinical symptoms suggesting allergic diseases, from november 2012 to february 2013 in marrakech. result ...201728270850
fungal root microbiome from healthy and brittle leaf diseased date palm trees (phoenix dactylifera l.) reveals a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites.in this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (bld) date palm trees (phoenix dactylifera l.) widely cultivated in arid zones of tunisia. a total of 52 endophytic fungi were isolated from healthy and bld roots of date palm trees, identified based on internal transcribed spacer-rdna sequence analysis and shown to represent 13 species belonging to fi ...201728293229
genetic characterization of libyan date palm resources by microsatellite markers.molecular typing of 377 female date palm trees belonging to 18 libyan cultivars and representing common genotypes in the central libyan oasis of al jufrah was performed using 16 highly polymorphic microsatellite or ssr loci. a total of 110 alleles with an average of 6.88 alleles per locus were scored indicating the high level of polymorphism existing among the cultivars thus allowing their genetic fingerprinting. moreover 28 alleles out of 110 were fixed. all the cultivars were characterized by ...201428324455
toxicity and detoxification mechanism of black pepper and its major constituent in controlling rhynchophorus ferrugineus olivier (curculionidae: coleoptera).the survival, feeding response, and detoxification mechanism of rhynchophorus ferrugineus olivier, a key pest responsible for destruction of date palm, was examined with different extracts of piper nigrum and its major constituent (piperine) identified by gc-ms. in the present study, toxicity of different extracts of black pepper was evaluated by incorporating diffferent doses of extracts into the artificial diet of red palm weevil larvae. all extracts showed dose-dependent insecticidal activity ...201728326461
development of molecular method for sex identification in date palm (phoenix dactylifera l.) plantlets using novel sex-linked microsatellite markers.microsatellite markers containing simple sequence repeats (ssrs) are a valuable tool for genetic analysis. date palm is a dioecious and slow flowering and is very difficult to identify the gender of the trees until it reaches the reproductive age (5-10 years). a total of 12 microsatellite primers were used with 30 date palm samples, 14 parents (8 male + 6 females) and 16 progeny (developed from parents breeding) which showed that microsatellites were highly polymorphic, having a great number of ...201628330092
optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. mejhoul.the effects of major mineral salts, l-glutamine, myo-inositol and carbon source on shoot bud proliferation of date palm (phoenix dactylifera l.) cv. mejhoul were evaluated. different concentrations of ammonium nitrate (nh4no3; 550, 825 or 1650 mg/l), potassium nitrate (kno3; 633.3, 950 or 1900 mg/l), calcium chloride dehydrate (cacl2·2h2o; 147, 220 or 440 mg/l), potassium dihydrogen phosphate (kh2po4; 57, 85 or 170 mg/l), magnesium sulfate heptahydrate (mgso4·7h2o; 123, 185 or 370 mg/l), l-gluta ...201628330181
genome-wide expression profiling in leaves and roots of date palm (phoenix dactylifera l.) exposed to salinity.date palm, as one of the most important fruit crops in north african and west asian countries including oman, is facing serious growth problems due to salinity, arising from persistent use of saline water for irrigation. although date palm is a relatively salt-tolerant plant species, its adaptive mechanisms to salt stress are largely unknown.201728330456
date palm waste-derived biochar composites with silica and zeolite: synthesis, characterization and implication for carbon stability and recalcitrant potential.engineered organo-mineral composites were synthesized from date palm waste biochar and silica or zeolite via mechanochemical treatments. date palm tree rachis (leaves) waste biomass was pre-treated with silica or zeolite minerals via ball milling and sonication prior to pyrolysis at 600 °c. the resultant organo-mineral composites and pristine materials were characterized using x-ray diffraction, thermogravimetric-differential thermal (tg-dta), fourier transform infrared, scanning electron micros ...201728337620
effect of various levels of date palm kernel on growth performance of broilers.the aim of this study was the assessment of various levels of date palm kernel (dpk) on the growth performance of broilers.201728344407
illumina miseq sequencing analysis of fungal diversity in stored dates.date palm has been a major fruit tree in the middle east over thousands of years, especially in the arabian peninsula. dates are consumed fresh (rutab) or after partial drying and storage (tamar) during off-season. the aim of the study was to provide in-depth analysis of fungal communities associated with the skin (outer part) and mesocarp (inner fleshy part) of stored dates (tamar) of two cultivars (khenizi and burny) through the use of illumina miseq sequencing.201728347268
anti hyperlipidemic and hepatoprotective effects of native date fruit variety "aseel" (phoenix dactylifera).diet rich in saturated fats and sugars have been associated with obesity, insulin resistance, hyperlipidemia and fatty liver disease. especially high serum lipid levels are directly connected to the progression of cardiovascular disorders, which are the leading cause of death all over the world. date palm fruit (phoenix dactylifera) is known for numerous health benefits however little information is available about in vivo clinical and preclinical benefits, hence antihyperlipidemic and hepatopro ...028375109
green biosynthesis of spherical silver nanoparticles by using date palm (phoenix dactylifera) fruit extract and study of their antibacterial and catalytic activities.in this work, we have synthesized spherical silver nanoparticles (ag nps) by a low-cost, rapid, simple and ecofriendly approach using date palm fruit extract as a novel natural reducing and stabilizing agent. the product was characterized by uv-visible spectroscopy, x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), field emission scanning electron microscopy (fesem), transmission electron microscopy (tem), atomic force microscopy (afm), energy-dispersive x-ray (edx) spectr ...028380222
identification and evaluation of antibacterial agents present in lipophilic fractions isolated from sub-products of phoenix dactilyfera.in this study, the dried powder of seeds and pollen of phoenix dactylifera was extracted using soxhlet extractor and biochemical composition of lipophilic extracts was analysed by gcms. then, we have tested the antibacterial effect of fatty acids and volatile compounds found in these sub-products. the results revealed that the main fatty acids found in deglet nour seeds is are oleic acid (42.06%) followed by capric acid (24.16%), takerbucht seeds extract contains mostly oleic acid (40.59%) and p ...201728403631
diversity of algerian oases date palm (phoenix dactylifera l., arecaceae): heterozygote excess and cryptic structure suggest farmer management had a major impact on diversity.date palm (phoenix dactyliferal.) is the mainstay of oasis agriculture in the saharan region. it is cultivated in a large part of the mediterranean coastal area of the sahara and in most isolated oases in the algerian desert. we sampled 10 oases in algeria to understand the structure of date palm diversity from the coastal area to a very isolated desert location. we used 18 microsatellite markers and a chloroplast minisatellite to characterize 414 individual palm trees corresponding to 114 named ...201728410422
date palm waste biochars alter a soil respiration, microbial biomass carbon, and heavy metal mobility in contaminated mined soil.a 30-day incubation experiment was conducted using a heavy metal-contaminated mined soil amended with date palm feedstock (fs) and its derivative biochars (bcs) at three pyrolysis temperatures of 300 (bc-300), 500 (bc-500), and 700 °c (bc-700) with different application rates (0.0, 5, 15, and 30 g kg-1) to investigate their short-term effects on soil respiration (co2-c efflux), microbial biomass carbon (mbc), soil organic carbon (soc), mobile fraction of heavy metals (cd, cu, pb, zn, mn, and fe) ...201728424945
somatic embryogenesis from bud and leaf explants of date palm (phoenix dactylifera l.) cv. najda.an efficient regeneration system through somatic embryogenesis was developed for date palm cv. najda. adventitious bud and proximal leaf segments cultured on murashige and skoog (ms) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. somatic embryogenesis induction seemed correlated with the type of the explant, the induction period and the auxin used. the highest rate of somatic embryogenesis (86.0%) was obtained on b ...201728444601
hydrothermal pretreatment and enzymatic hydrolysis of mixed green and woody lignocellulosics from arid regions.utilization of multi-specie feedstocks is imperative for application of lignocellulosic biorefineries in arid regions. different lignocellulosic residues vary in composition and anatomical features. pretreatment and enzymatic hydrolysis are two processes at the front end of any lignocellulosics biorefinery applying biochemical pathway, and have to efficiently deal with the variance in the feedstock composition and properties. however, there is limited knowledge about effect of mixing different l ...201728456045
date stones of phoenix dactylifera and jujube shells of ziziphus lotus as potential biosorbents for anionic dye removal.this study investigated the biosorption of congo red (cr) from aqueous solution by date stones (ds) of phoenix dactylifera and jujube shells (js) of ziziphus lotus. batch operations were carried out in the liquid phase to observe the effect of various experimental parameters such as contact time, ph, temperature, and initial dye concentration on removal of cr. the characteristics of the ds and js were also examined by fourier transform infrared analysis. the biosorption data of cr on ds and js s ...201728463549
in silico approach for characterization and comparison of repeats in the genomes of oil and date palms.transposable elements (tes) are mobile genetic elements present in almost all eukaryotic genomes. due to their typical patterns of repetition, discovery, and characterization, they demand analysis by various bioinformatics software. probably, as a result of the need for a complex analysis, many genomes publicly available do not have these elements annotated yet. in this study, a de novo and homology-based identification of tes and microsatellites was performed using genomic data from 3 palm spec ...201728469420
ethnobotanical survey of phoenix dactylifera l. pollen used for the treatment of infertility problems in algerian oases.the phoenix dactylifera l. (date palm) is known for its traditional medicinal properties across the history of native population in algerian sahara. there is a large trend of consumption of date palm pollen preparations in many human infertility cases in our country. however, the validity has not been scientifically tested. there has been no direct scientific research on this application. this study was undertaken to identify cultivars with greater potential in the traditional medicine uses. to ...201728480429
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
monitoring and mass-trapping methodologies using pheromones: the lesser date moth batrachedra amydraula.the lesser date moth (ldm) batrachedra amydraula is a significant pest of date palm fruits. previously, detection and monitoring of the pest was inaccurate due to high costs of sampling with lifting machines. we report a practical system for detection and monitoring of ldm based on pheromone traps and relevant models. dose-response experiments with ldm pheromone traps indicated a 1 mg lure is optimal for monitoring. delta traps with adhesive covering their entire inner surface gave the highest c ...028490389
the use of high throughput dna sequence analysis to assess the endophytic microbiome of date palm roots grown under different levels of salt stress.date palms are able to grow under diverse abiotic stress conditions including in saline soils, where microbial communities may be help in the plant's salinity tolerance. these communities able to produce specific growth promoting substances can enhance date palm growth in a saline environment. however, these communities are poorly defined. in the work reported here, the date palm endophytic bacterial and fungal communities were identified using the pyrosequencing method, and the microbial differ ...201628494084
charting novel allergens from date palm pollen (phoenix sylvestris) using homology driven proteomics.pollen grains from phoenix sylvestris (date palm), a commonly cultivated tree in india has been found to cause severe allergic diseases in an increasing percentage of hypersensitive individuals. to unearth its allergenic components, pollen protein were profiled by two-dimensional gel electrophoresis followed by immunoblotting with date palm pollen sensitive patient sera. allergens were identified by maldi-tof/tof employing a layered proteomic approach combining conventional database dependent se ...201728535932
impact of environmental variables on dubas bug infestation rate: a case study from the sultanate of oman.date palm cultivation is economically important in the sultanate of oman, with significant financial investment coming from both the government and from private individuals. however, a global infestation of dubas bug (ommatissus lybicus bergevin) has impacted the middle east region, and infestations of date palms have been widespread. in this study, spatial analysis and geostatistical techniques were used to model the spatial distribution of dubas bug infestations to (a) identify correlations be ...201728558069
detecting early signs of heat and drought stress in phoenix dactylifera (date palm).plants adapt to the environment by either long-term genome evolution or by acclimatization processes where the cellular processes and metabolism of the plant are adjusted within the existing potential in the genome. here we studied the adaptation strategies in date palm, phoenix dactylifera, under mild heat, drought and combined heat and drought by transcriptomic and metabolomic profiling. in transcriptomics data, combined heat and drought resembled heat response, whereas in metabolomics data it ...201728570677
date palm tree (phoenix dactylifera l.): natural products and therapeutic options.many plants, including some of the commonly consumed herbs and spices in our daily food, can be safely and effectively used to prevent and/or treat some health concerns. for example, caffeine the active ingredient found in coffee beans (coffea), shows biological activity in the treatment of the central nervous system (cns) disorders, indole-3-carbinol, and 3,3'-diindolylmethane are both broccoli (brassica oleracea) derived phytochemicals with potential anti-cancer activity, and resveratrol, isol ...028588600
apparent metabolizable energy value of whole date palm (phoenix dactylifera l.) and its possible use as a feedstuff for aged laying hens.this study was conducted to evaluate the feeding value of whole date palm (wdp) with and without enzyme supplementation on aged laying hen's performance. apparent metabolizable energy value of wdp was determined by the total collection method using ten adult leghorn cockerels. wdp was substituted with a corn-soybean meal basal diet at 40% level, and then the metabolizable energy of this experimental diet and basal diet was determined. after that, a total number of 256 bovanz 95-week-old hens wer ...201728597141
antibacterial activities of diospyros blancoi, phoenix dactylifera and morus nigra against dental caries causing pathogens: an in vitro study.the study was undertaken to determine the in-vitro antibacterial potential of diospyrosblancoi, phoenix dactylifera and morusnigra leaf extracts in hexane, chloroform, methanol, ethyl-acetate and aqueous extracts against dental caries causing bacteria. disc diffusion assay was used to determine the antibacterial efficacy; the extracts were further separated using thin layer chromatography and the anti-biofilm activity of the extracts was also determined. the preliminary phytochemical screening o ...201728603127
nipah virus outbreaks in bangladesh: a deadly infectious disease.during 2001-2011, multidisciplinary teams from the institute of epidemiology, disease control and research (iedcr) and international centre for diarrhoeal disease research, bangladesh(icddr,b) identified sporadic cases and 11 outbreaks of nipah encephalitis. three outbreaks were detected through sentinel surveillance; others were identified through event-based surveillance. a total of 196 cases of nipah encephalitis, in outbreaks, clusters and as isolated cases were detected from 20 districts of ...201728612796
a survey on chemical constituents and indications of aromatic waters soft drinks (hydrosols) used in persian nutrition culture and folk medicine for neurological disorders and mental health.in persian nutrition culture, drinking aromatic waters (hydrosols, distillate) has a long history as functional beverages or therapeutic remedies. the co-distilled water with essential oils, which contains partial amounts of more water-soluble volatile compounds are diluted and used as beverages. since the solubility of volatile components is different in water, the overall composition, and thus the biological activities of aromatic waters seem to be different from the essential oils they were c ...028633539
cardiopreventive effect of ethanolic extract of date palm pollen against isoproterenol induced myocardial infarction in rats through the inhibition of the angiotensin-converting enzyme.the present study aimed to examine the putative preventive effect of the ethanolic extract date palm pollen (dpp, phoenix dactylifera l., family arecaceae) on isoproterenol-induced myocardial infarction (mi) in rats. twenty four rats were randomly divided into four groups including control. they were treated with dpp extract (400mg/kg) and clopidogrel (0.2mg/kg) for 7days followed by myocardial injury induction using subcutaneous isoproterenol (100mg/kg) with an interval of 24h for two days (6th ...201728645882
a large-scale behavior change intervention to prevent nipah transmission in bangladesh: components and costs.nipah virus infection (niv) is a bat-borne zoonosis transmitted to humans through consumption of niv-contaminated raw date palm sap in bangladesh. the objective of this analysis was to measure the cost of an niv prevention intervention and estimate the cost of scaling it up to districts where spillover had been identified.201728651646
report - screening of the anti-hyperglycemic activity of some medicinal plants of jordan.diabetes represents a group of common diseases that are characterized by dysregulation of blood glucose levels. plants are traditionally used for management of diseases including diabetes. in this study, we screened the anti-diabetic effect of extracts of 21 plants grown in jordan. extracts of plants were screened for their antihyperglycemic activity. diabetes was induced in sprague dawley rats using alloxan. plant extracts were dosed at 1gm/kg. blood glucose was measured at baseline and at ever ...201728653938
synthetic co-attractants of the aggregation pheromone of the date palm root borer oryctes agamemnon.laboratory and field investigations to identify and evaluate plant co-attractants of the aggregation pheromone of the date palm pest oryctes agamemnon are reported. volatiles emitted by freshly cut palm core and palm core with feeding males, were collected, analyzed by gas chromatography coupled to mass spectrometry and evaluated in olfactometers alone or combined with synthetic pheromone. a collection of palm odor without male effluvia was attractive alone and enhanced attraction to synthetic p ...201728667548
the desert plant phoenix dactylifera closes stomata via nitrate-regulated slac1 anion channel.date palm phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. how are palms under such harsh environmental conditions able to limit transpirational water loss? here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from p. dactylifera. to understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast ab ...201728670699
antioxidant system response and cdna-scot marker profiling in phoenix dactylifera l. plant under salinity stress.many phoenix dactylifera (date palm) cultivars are grown in the arid and semiarid regions of the world, including saudi arabia. p. dactylifera is highly tolerant to salinity stress. to investigate the response of khalas cultivar of p. dactylifera, two-month-old plants were treated with sodium chloride (50, 100, and 150 mm nacl) for three months. our result showed that proline content was higher in all treated plants compared to control plants. thiobarbituric acid reactive substances (tbars) were ...201728702461
the discovery of wild date palms in oman reveals a complex domestication history involving centers in the middle east and africa.for many crops, wild relatives constitute an extraordinary resource for cultivar improvement [1, 2] and also help to better understand the history of their domestication [3]. however, the wild ancestor species of several perennial crops have not yet been identified. perennial crops generally present a weak domestication syndrome allowing cultivated individuals to establish feral populations difficult to distinguish from truly wild populations, and there is frequently ongoing gene flow between wi ...201728712568
plant domestication: wild date palms illuminate a crop's sticky origins.in a new study, previously unknown populations of wild date palm have been identified in remote areas of oman. genomic analyses indicate date palm domestication occurred in the eastern portion of the arabian peninsula and reveal substantial subsequent gene flow with african palm populations.201728743015
storage and viability assessment of date palm pollen.pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. here we describe pollen storage methods, such as room temperature (25-30 °c), refrigeration (4 °c), storage at 4 °c in desiccators, deep freezer (-20 °c), and cryopreservation (-196 °c). b ...201728755209
in vitro conservation of date palm tissue cultures.in vitro technology offers a potential solution for the conservation of date palm germplasm. slow growth induced by low temperature allows storage from several months up to few years. otherwise, cryopreservation is suitable for long-term in vitro conservation, at between -79 and -196 °c. this chapter describes a protocol for cold storage at 5 °c and cryopreservation of date palm tissue cultures. for cold storage, 70% of shoot buds remain healthy after storing for 12 months at 5 °c, and callus cu ...201728755210
cryopreservation of date palm pro-embryonic masses using the d cryo-plate technique.in this chapter, we describe a cryopreservation (liquid nitrogen, -196 °c) protocol developed for long-term storage of date palm pro-embryonic masses (pems), which uses the recently established d cryo-plate technique. clumps of pems (3-5 mm in size) were dissected from pem cultures and placed on pretreatment medium containing 171 g/l sucrose for 3 days. clumps were placed in the wells of aluminum cryo-plates in which they were made to adhere using droplets of 3% calcium alginate. pems were treat ...201728755211
in vitro cryopreservation of date palm caulogenic meristems.cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. in this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free ms medium. the positive impact of sucrose precul ...201728755212
in vitro conservation of date palm shoot-tip explants and callus cultures under minimal growth conditions.date palm fruit production has great economic significance for many countries. there is a fundamental necessity to conserve valuable date palm germplasm, but there are various problems with in vivo and ex situ conservation. in vitro storage has several advantages over conventional germplasm conservation methods. the in vitro technique offers a developed method of slow-growth storage, which is considered as an alternate solution for short- and medium-term storage of date palm germplasm under cont ...201728755213
in vitro conservation of date palm somatic embryos using growth-retardant conditions.in vitro conservation is carried out to maintain disease-free genetic materials, in a small area, protected against pests, insects, soil problems (alkaline, acidic, excess salinity, lack of organic matter, too dry, or too wet), climatic changes, and high-multiplication potential. a requirement of successful in vitro conservation is that the plants can be regenerated into complete plants rapidly when desired. the current work describes in vitro propagation and conservation techniques employing sl ...201728755214
encapsulation of date palm somatic embryos: synthetic seeds.synthetic seed or encapsulated somatic embryos may be used for propagation, storage, and exchange of plant germplasm and have many diverse applications in date palm cultivation. they have advantages over conventional use of offshoot material for germplasm propagation, maintenance, exchange, and transportation. this chapter describes a protocol for date palm synthetic seed production by encapsulation of somatic embryos with sodium alginate. among three concentrations used, 3% sodium alginate foll ...201728755215
evaluation of clonal fidelity of micropropagated date palm by random amplified polymorphic dna (rapd).date palm is a fruit-bearing tree commonly found in arid and semiarid regions. it is a dioecious plant, producing fruit on female plants and a limited number of basal offshoots for propagation. to produce large numbers of uniform plantlets, tissue culture techniques are required. it is highly advisable to detect genetic variation that may occur through micropropagation techniques as it may lead to phenotypic alterations. random amplified polymorphic dna (rapd) is a simple and pcr-based molecular ...201728755216
molecular identification of fungal contamination in date palm tissue cultures.fungal contamination of in vitro cultures of date palm (phoenix dactylifera l.) is the major constraint to their initiation and maintenance. different molecular approaches have been applied successfully to analyze both inter- and intraspecific variation among fungal species as well as determine their identity. this chapter describes step-by-step procedures of molecular identification of fungal contaminants by internal transcribed spacer (its) products of the most common fungal contaminants of da ...201728755217
genetic diversity analysis of date palm using random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr).random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) are pcr-based molecular techniques used for evaluation and characterization of date palm to find the best genotype and male/female identification at an early stage. genetic fingerprinting using molecular markers is an important tool for the analysis of genetic diversity and cultivar identification. here, we present an improved dna extraction protocol using leaf tissue, based on the standard cetyltrimethyl ammonium br ...201728755218
date palm genetic diversity analysis using microsatellite polymorphism.date palm (phoenix dactylifera l.) is considered one of the great socioeconomic resources in the middle east and the arab regions. the tree has been and still is at the center of the comprehensive agricultural development. the number of known date palm cultivars, distributed worldwide, is approximately 3000. the success of genetic diversity conservation or any breeding program depends on an understanding of the amount and distribution of the genetic variation already in existence in the genetic ...201728755219
assessing date palm genetic diversity using different molecular markers.molecular marker technologies which rely on dna analysis provide powerful tools to assess biodiversity at different levels, i.e., among and within species. a range of different molecular marker techniques have been developed and extensively applied for detecting variability in date palm at the dna level. recently, the employment of gene-targeting molecular marker approaches to study biodiversity and genetic variations in many plant species has increased the attention of researchers interested in ...201728755220
molecular analysis of date palm genetic diversity using random amplified polymorphic dna (rapd) and inter-simple sequence repeats (issrs).the date palm is an ancient domesticated plant with great diversity and has been cultivated in the middle east and north africa for at last 5000 years. date palm cultivars are classified based on the fruit moisture content, as dry, semidry, and soft dates. there are a number of biochemical and molecular techniques available for characterization of the date palm variation. this chapter focuses on the dna-based markers random amplified polymorphic dna (rapd) and inter-simple sequence repeats (issr ...201728755221
determining phylogenetic relationships among date palm cultivars using random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers.investigation of genetic variation and phylogenetic relationships among date palm (phoenix dactylifera l.) cultivars is useful for their conservation and genetic improvement. various molecular markers such as restriction fragment length polymorphisms (rflps), simple sequence repeat (ssr), representational difference analysis (rda), and amplified fragment length polymorphism (aflp) have been developed to molecularly characterize date palm cultivars. pcr-based markers random amplified polymorphic ...201728755222
genotyping and molecular identification of date palm cultivars using inter-simple sequence repeat (issr) markers.molecular markers are credible for the discrimination of genotypes and estimation of the extent of genetic diversity and relatedness in a set of genotypes. inter-simple sequence repeat (issr) markers rapidly reveal high polymorphic fingerprints and have been used frequently to determine the genetic diversity among date palm cultivars. this chapter describes the application of issr markers for genotyping of date palm cultivars. the application involves extraction of genomic dna from the target cu ...201728755223
molecular identification of date palm cultivars using random amplified polymorphic dna (rapd) markers.ambiguity in the total number of date palm cultivars across the world is pointing toward the necessity for an enumerative study using standard morphological and molecular markers. among molecular markers, dna markers are more suitable and ubiquitous to most applications. they are highly polymorphic in nature, frequently occurring in genomes, easy to access, and highly reproducible. various molecular markers such as restriction fragment length polymorphism (rflp), amplified fragment length polymo ...201728755224
early sex identification in date palm by male-specific sequence-characterized amplified region (scar) markers.date palm (phoenix dactylifera l.) is a dioecious plant, and sex of the seedlings can be determined only at the time of first flowering which takes 4-5 years. female date palm plants are of economic importance as they bear the fruit. therefore, sex identification at an early stage is highly desirable. dna-based markers are useful for early sex detection. in this chapter, we describe male-specific sequence-characterized amplified region (scar) markers to identify sex in date palm at the seedling ...201728755225
gender identification in date palm using molecular markers.breeding of date palm is complicated because of its long life cycle and heterozygous nature. sexual propagation of date palm does not produce true-to-type plants. sex of date palms cannot be identified until the first flowering stage. molecular markers such as random amplified polymorphic dna (rapd), sequence-characterized amplified regions (scar), and simple sequence repeats (ssr) have successfully been used to identify the sex-linked loci in the plant genome and to isolate the corresponding ge ...201728755226
development of sex-specific pcr-based markers in date palm.molecular markers are used efficiently in the development and identification of gender-specific pcr-based markers in date palm. there is mounting evidence that different marker systems vary in their mechanisms of detecting polymorphism and genome coverage. therefore, they could complement each other to generate accurate sex-specific markers in date palm. this chapter describes the uses of pcr-based molecular markers to develop and identify the gender in different date palm genotypes; these are a ...201728755227
date palm sex differentiation based on fluorescence in situ hybridization (fish).in situ hybridization (ish) is used to visualize defined dna sequences in cellular preparations by hybridization of complementary probe sequences. recently, the fluorescence in situ hybridization (fish) technique has become a powerful and useful tool for the direct detection of specific dna fragments in the genome. ribosomal dna genes (45s and 5s rdna) are commonly used as markers for the physical mapping of plant chromosomes to analyze genomic organization. here we describe cytological-based ma ...201728755228
characterization and amplification of gene-based simple sequence repeat (ssr) markers in date palm.the paucity of molecular markers limits the application of genetic and genomic research in date palm (phoenix dactylifera l.). availability of expressed sequence tag (est) sequences in date palm may provide a good resource for developing gene-based markers. this study characterizes a substantial fraction of transcriptome sequences containing simple sequence repeats (ssrs) from the est sequences in date palm. the est sequences studied are mainly homologous to those of elaeis guineensis and musa a ...201728755229
mitochondrial molecular markers for resistance to bayoud disease in date palm.bayoud disease, caused by fusarium oxysporum f. sp. albedinis, is a very serious and destructive disease to date palm. screening of date palm germplasm for resistance to bayoud disease is a crucial step to avoid or alleviate the disease consequences. fortunately, it was discovered that there are two mitochondrial plasmid-like dna molecules associated with susceptibility or resistance to bayoud disease. in this chapter, we present a fast, simple, and reliable technique to screen date palm germpla ...201728755230
analysis of expressed sequence tags (est) in date palm.expressed sequence tags (est) were generated from a normalized cdna library of the date palm sukkari cv. to understand the high-quality and better field performance of this well-known commercial cultivar. a total of 6943 high-quality ests were generated, out of them 6671 are submitted to the genbank dbest (libest_028537). the generated ests were assembled into 6362 unigenes, consisting of 494 (14.4%) contigs and 5868 (84.53%) singletons. the functional annotation shows that the majority of the e ...201728755231
development of genomic simple sequence repeats (ssr) by enrichment libraries in date palm.development of highly informative markers such as simple sequence repeats (ssr) for cultivar identification and germplasm characterization and management is essential for date palms genetic studies. the present study documents the development of ssr markers and assesses genetic relationships of commonly grown date palm (phoenix dactylifera l.) cultivars in different geographical regions of saudi arabia. a total of 93 novel simple sequence repeat (ssr) markers were screened for their ability to d ...201728755232
microrna expression in multistage date fruit development.micrornas (mirnas) are small endogenous noncoding rnas. plant mirnas are known to play important regulatory roles in homeostasis, stress response, and diverse developmental processes. here, we describe the identification of conserved mirnas in date palm (phoenix dactylifera l.) based on transcriptomic data acquired across multistage fruit development and genome sequences, which include 238 plant conserved mirnas and 276 novel p. dactylifera-specific mirnas.201728755233
proteome of abiotic stress tolerance in date palm.this protocol describes the analysis of date palm proteome under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. three-month-old date palm seedlings are subjected to drought (27.5 g/l polyethylene glycol 6000) and salinity stress conditions (16 g/l nacl) for 1 month prior to leaf sample analysis. differential in gel electrophoresis (dige) analysis of protein extracts identifies the sensitive proteins that respond to abiotic stress. mass spectrom ...201728755234
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