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characterization and mapping of non-s gametophytic self-compatibility in sweet cherry (prunus avium l.).self-incompatibility in prunus (rosaceae) species, such as sweet cherry, is controlled by a multiallelic locus (s), in which two tightly linked genes, s-rnase and sfb (s haplotype-specific f-box), determine the specificity of the pollen and the style. fertilization in these species occurs only if the s-specificities expressed in the pollen and the pistils are different. however, modifier genes have been proposed to be necessary for a full manifestation of the self-incompatibility response. 'cris ...201121127024
effect of the commercial ripening stage and postharvest storage on microbial and aroma changes of 'ambrunés' sweet cherries.the purpose of this work was to investigate the effect of the commercial ripening stage and postharvest storage on microbial and aroma changes of 'ambrunés' sweet cherries. the microbial counts and volatile profile of sweet cherry batches automatically sanitized and classified in three commercial ripening stages were studied for five postharvest storages. the batches were also evaluated sensorially, and the correlations between volatile compounds and aroma quality were determined. the microbial ...201023654239
cloning and expression analysis of cdnas for aba 8'-hydroxylase during sweet cherry fruit maturation and under stress conditions.abscisic acid (aba) plays a key role in various aspects of plant growth and development, including adaptation to environmental stress and fruit maturation in sweet cherry fruit. in higher plants, the level of aba is determined by synthesis and catabolism. in order to gain insight into aba synthesis and catabolism in sweet cherry fruit during maturation and under stress conditions, four cdnas of paccyp707a1 -paccyp707a4 for 8'-hydroxylase, a key enzyme in the oxidative catabolism of aba, and one ...201020728961
population structure and genetic bottleneck in sweet cherry estimated with ssrs and the gametophytic self-incompatibility locus.domestication and breeding involve the selection of particular phenotypes, limiting the genomic diversity of the population and creating a bottleneck. these effects can be precisely estimated when the location of domestication is established. few analyses have focused on understanding the genetic consequences of domestication and breeding in fruit trees. in this study, we aimed to analyse genetic structure and changes in the diversity in sweet cherry prunus avium l.201020727153
self-compatibility in 'cristobalina' sweet cherry is not associated with duplications or modified transcription levels of s-locus genes.sweet cherry shows s-rnase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. the specificity of the self-incompatible reaction is determined by two genes located in the s-locus. these encode a pistil-expressed ribonuclease (s-rnase) that inhibits self pollen tube growth, and a pollen-expressed f-box protein (sfb) that may be involved in the cytotoxicity of self-s-rnases. initial genetic and pollination studies in a self ...201020411390
sweet cherry pomological and biochemical characteristics influenced by rootstock.fruits of lapins sweet cherry (prunus avium l.) from 12-year-old trees on different rootstocks (f 12/1, maxma 14, piku 1, edabriz, gisela 5, gisela 195/20, weiroot 13, weiroot 158, and weiroot 72) were analyzed for pomological (fruit weight, % flesh of fruit weight, soluble solids content, titratable acidity, and firmness) and biochemical parameters (individual sugars, organic acids, and phenolic compounds) considering yield. for the first time, two procyanidins have been identified in sweet che ...201020337477
effect of prunus avium roots on river bank strong.a pulling effect by side roots is one way in which roots help to side in-plane strong of a little depth soil mass. in contrast to the effect of vertically-enlarge roots, whereby soil is strengthened by an increase in its shear strength, the pulling effect strengthens the soil by increasing the tensile strength of the rooted soil zone. to verify whether or not a pulling effect exists in the root system of prunus avium in the roudsar, north iran and to study the importance and size of this effect, ...200818819551
influence of genotype-temperature interaction on pollen performance.pollen competition and selection have significant evolutionary consequences, but very little is known about how they can be modulated. we have examined in cherry (prunus avium l.) how pollen performance is affected by the genotype of the pollen and by the environmental conditions under which it grows, namely the pistilar tissue and temperature. the different pollen donor genotypes tested in this work differed in their behaviour both in vitro and in vivo and this behaviour was modulated depending ...200516313462
basic rnases of wild almond (prunus webbii): cloning and characterization of six new s-rnase and one "non-s rnase" genes.in order to investigate the s-rnase allele structure of a prunus webbii population from the montenegrin region of the balkans, we analyzed 10 prunus webbii accessions. we detected 10 different s-rnase allelic variants and obtained the nucleotide sequences for six s-rnases. the blast analysis showed that these six sequences were new prunus webbii s-rnase alleles. it also revealed that one of sequenced alleles, s(9)-rnase, coded for an amino acid sequence identical to that for prunus dulcis s(14)- ...200918778875
genetic and molecular characterization of three novel s-haplotypes in sour cherry (prunus cerasus l.).tetraploid sour cherry (prunus cerasus l.) exhibits gametophytic self-incompatibility (gsi) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, s-rnase and sfb (s haplotype-specific f-box protein gene), respectively. as sour cherry selections can be either self-compatible (sc) or self-incompatible (si), polyploidy per se does not result in sc. instead the genotype-dependent loss of si in sour cherry is due to the accumulation of n ...200818617504
[characteristics of urea 15n absorption, allocation, and utilization by sweet-cherry (prunus avium l.)].with five-year old 'zaodaguo' sweet-cherry (prunus avium l.) as test material, this paper studied the characteristics of its urea 15n absorption, allocation, and utilization when applied before bud-break. the results showed that the ndff of different organs increased gradually with time, and was higher in fine roots and storage organs at full-blooming stage. at fruit core-hardening stage, the ndff of long shoots and leaves increased quickly, reaching to 0.72% and 0.59% , respectively. from fruit ...200818533545
rootstock-induced dwarfing in cherries is caused by differential cessation of terminal meristem growth and is triggered by rootstock-specific gene regulation.use of dwarfing rootstocks has dramatically increased the profitability of fruit production by reducing production costs, reduced chemical use and higher density plantings. despite the importance of rootstock-induced dwarfing, the cause of this phenomenon is not known. using two commercially available graft combinations consisting of a sweet cherry scion, 'bing', on a dwarfing rootstock (gi5) or a semi-vigorous rootstock (gi6), we discovered that the difference in grafted tree height was due to ...200919429629
in vitro bioavailability of phenolic compounds from five cultivars of frozen sweet cherries (prunus avium l.).the bioavailability of phenolic compounds from five cultivars of frozen sweet cherries was assessed by a digestion process involving pepsin-hcl digestion (to simulate gastric digestion) and pancreatin digestion with bile salts (to simulate small intestine conditions) and dialyzed to assess serum- and colon-available fractions. after pepsin digestion, the % recovery of total phenolics, relative to the original starting material, increased, whereas the % anthocyanins did not change. following panc ...200818459792
mechanical stimuli regulate the allocation of biomass in trees: demonstration with young prunus avium trees.plastic tree-shelters are increasingly used to protect tree seedlings against browsing animals and herbicide drifts. the biomass allocation in young seedlings of deciduous trees is highly disturbed by common plastic tree-shelters, resulting in poor root systems and reduced diameter growth of the trunk. the shelters have been improved by creating chimney-effect ventilation with holes drilled at the bottom, resulting in stimulated trunk diameter growth, but the root deficit has remained unchanged. ...200818448448
unequal allelic frequencies at the self-incompatibility locus within local populations of prunus avium l.: an effect of population structure?in this paper, we investigated the genetic structure and distribution of allelic frequencies at the gametophytic self-incompatibility locus in three populations of prunus avium l. in line with theoretical predictions under balancing selection, genetic structure at the self-incompatibility locus was almost three times lower than at seven unlinked microsatellites. furthermore, we found that s-allele frequencies in wild cherry populations departed significantly from the expected isoplethic distribu ...200818284513
a new self-compatibility haplotype in the sweet cherry 'kronio', s5', attributable to a pollen-part mutation in the sfb gene.'kronio' is a sicilian cultivar of sweet cherry (prunus avium), nominally with the incompatibility genotype s(5)s(6), that is reported to be naturally self-compatible. in this work the cause of its self-compatibility was investigated. test selfing confirmed self-compatibility and provided embryos for analysis; pcr with consensus primers designed to amplify s-rnase and sfb alleles showed that the embryos were of two types, s(5)s(5) and s(5)s(6), indicating that s(6) pollen failed, but s(5) succee ...200718182436
self-incompatibility of prunus tenella and evidence that reproductively isolated species of prunus have different sfb alleles coupled with an identical s-rnase allele.many species of prunus display an s-rnase-based gametophytic self-incompatibility (si), controlled by a single highly polymorphic multigene complex termed the s-locus. this comprises tightly linked stylar- and pollen-expressed genes that determine the specificity of the si response. we investigated si of prunus tenella, a wild species found in small, isolated populations on the balkan peninsula, initially by pollination experiments and identifying stylar-expressed rnase alleles. nine p. tenella ...200717461794
the s haplotype-specific f-box protein gene, sfb, is defective in self-compatible haplotypes of prunus avium and p. mume.many prunus species, including sweet cherry and japanese apricot, of the rosaceae, display an s-rnase-based gametophytic self-incompatibility (gsi). the specificity of this outcrossing mechanism is determined by a minimum of two genes that are located in a multigene complex, termed the s locus, which controls the pistil and pollen specificities. sfb, a gene located in the s locus region, encodes an f-box protein that has appropriate s haplotype-specific variation to be the pollen determinant in ...200415272875
cracking susceptibility of sweet cherries (prunus avium l.) under different conditions.rain and hail during ripening and harvesting season can cause yield losses up to 90 % in sweet cherry cultivations in germany. particularly, high yield losses after precipitation are due to the cracking and the following rotting process through bacteria and fungi's. in order to protect the crop and improve cherry quality they can be treated with chemicals dried or healthier covered with rain shelters. to date the cracking phenomenon of cherries is still not clearly understood. therefore, in the ...200617390796
composition of the cuticle of developing sweet cherry fruit.the composition of wax and cutin from developing sweet cherry (prunus avium) fruit was studied by gc-ms between 22 and 85 days after full bloom (dafb). in this and our previous study, fruit mass and surface area increased in a sigmoidal pattern with time, but mass of the cuticular membrane (cm) per unit fruit surface area decreased. on a whole fruit basis, mass of cm increased up to 36 dafb and remained constant thereafter. at maturity, triterpenes, alkanes and alcohols accounted for 75.6%, 19.1 ...200717328933
influence of cultivar and processing on cherry (prunus avium) allergenicity.oral allergy syndrome is an immediate food allergic event that affects lips, mouth, and pharynx, is often triggered by fruits and vegetables, and may be associated with pollinosis. here, we report on the allergenic pattern of different varieties of cherry (prunus avium) and results obtained by applying several technological processes to the selected varieties. whole cherries were submitted to chemical peeling, thermal treatment, and syruping processes, and the relative protein extracts were anal ...200617177523
detecting local establishment strategies of wild cherry (prunus avium l.).p. avium, a pioneer tree species that colonizes early forest successional stages, is assumed to require an effective strategy allowing stably repeatable rounds of local establishment, dispersal and local extinction. consequently, the early replacement of cherry by climax tree species makes the establishment of several local generations very unlikely, especially in central european continuous cover forests. this has to be seen in connection with the mixed reproduction system involving asexual rep ...200617020607
the mutated s1-haplotype in sour cherry has an altered s-haplotype-specific f-box protein gene.gametophytic self-incompatibility (gsi) is an outcrossing mechanism in flowering plants that is genetically controlled by 2 separate genes located at the highly polymorphic s-locus, termed s-haplotype. this study characterizes a pollen part mutant of the s(1)-haplotype present in sour cherry (rosaceae, prunus cerasus l.) that contributes to the loss of gsi. inheritance of s-haplotypes from reciprocal interspecific crosses between the self-compatible sour cherry cultivar ujfehértói fürtös carryin ...200616985081
heterozygote excess in a self-incompatible and partially clonal forest tree species -- prunus avium l.wild cherry (prunus avium l.), a partially asexual self-incompatible forest tree, shows heterozygote excess, which is a poorly studied phenomenon. in three natural populations, we found significant heterozygote excess at almost all investigated loci (eight microsatellites and markers for the self-incompatibility locus). we examined four hypotheses to account for this observed heterozygote excess. first, negative f(is) can result from a lack of selfed progeny in small populations of outcrossing s ...200616780428
chitosan acetate as an active coating material and its effects on the storing of prunus avium l.in this article, chitosan acetate (ca) was prepared by the method of solid-liquid reaction. ca was a stable faint yellow powder with water solubility. ca kept the same backbone in the chemical structure as the raw material of chitosan, and it also had the similar antibacterial properties with chitosan. ca could form a coating film on the outside surface of the sweet cherries, could effectively retard the loss of the water, titratable acidity, and ascorbic acid of sweet cherries, and could induce ...201020492258
assignation of sweet cherry selections to 3 taste groupings based on perceived sweetness and sourness.providing consumers with basic taste properties of sweet cherries at point of purchase would allow consumers to make purchase decisions based on fruit's intrinsic sensory attributes. the objective of this study was to develop a model to predict taste-grouping assignation of cherries into the following categories: (1) low sweetness/high sourness, (2) balance between sweetness and sourness, and (3) high sweetness/low sourness. a sensory panel (n = 10) was trained to recognize sweetness and sournes ...201420492202
phenolic compounds in cherry ( prunus avium ) heartwood with a view to their use in cooperage.the phenolic and tannic composition of heartwood extracts from prunus avium , commonly known as cherry tree, before and after toasting in cooperage were studied using hplc-dad and hplc-dad/esi-ms. nonflavonoid (16 compounds) and flavonoid (27 compounds) polyphenols were identified, 12 of them in only a tentative way. the nonflavonoids found were lignin constituents, and their pattern is different compared to oak, since they include compounds such as protocatechuic acid and aldehyde, p-coumaric a ...201020302370
allele-specific pcr detection of sweet cherry self-incompatibility (s) alleles s1 to s16 using consensus and allele-specific primers.pcr-based identification of all 13 known self-incompatibility (s) alleles of sweet cherry is reported. two pairs of consensus primers were designed from our previously published cdna sequences of s(1) to s(6) s-rnases, the stylar components of self-incompatibility, to reveal length variation of the first and the second introns. with the exception of the first intron of s(13), these also amplified s(7) to s(14) and an allele previously referred to as s(x), which we now label s(16). the genomic pc ...200314523529
diuretic effect of powdered cerasus avium (cherry) tails on healthy volunteers.in this study, the diuretic activity of powdered cherry stalk was evaluated in 13 healthy volunteers by means of their water balance. in addition to biochemical parameters, such as urinary electrolyte concentration, osmolality and any adverse reaction were determined. the capsules of cherry stalks were administered at an equivalent dose of 2.0 grams of the plant per person. urinary biochemical determination was made of concentration of electrolytes (sodium, potassium, chloride and calcium), urin ...200919783515
influence of habitat structure and resource availability on the movements of rhagoletis indifferens (diptera: tephritidae).habitat structure and resources availability may differentially influence movement between habitat patches. we examined fly movement decisions (stay or leave) at the scale of individual trees by measuring the response of marked rhagoletis indifferens (diptera: tephritidae) to sweet cherry trees (prunus avium) that were manipulated by changing the shape of the tree (structure treatment = normal or reduced) and the fruit load (fruit treatment = augmented, normal, or reduced). more than 600 observa ...200919508793
inheritance and interactions of incompatibility alleles in the tetraploid sour cherry.three progenies of sour cherry (prunus cerasus) were analysed to correlate self-(in)compatibility status with s-rnase phenotype in this allotetraploid hybrid of sweet and ground cherry. self-(in)compatibility was assessed in the field and by monitoring pollen tube growth after selfing. the s-rnase phenotypes were determined by isoelectric focusing of stylar proteins and staining for rnase activity and, for the parents, confirmed by pcr. seedling phenotypes were generally consistent with disomic ...200616307228
scion-rootstock interaction affects the physiology and fruit quality of sweet cherry.water relations, leaf gas exchange, chlorophyll a fluorescence, light canopy transmittance, leaf photosynthetic pigments and metabolites and fruit quality indices of cherry cultivars 'burlat', 'summit' and 'van' growing on five rootstocks with differing size-controlling potentials that decrease in the order: prunus avium l. > cab 11e > maxma 14 > gisela 5 > edabriz, were studied during 2002 and 2003. rootstock genotype affected all physiological parameters. cherry cultivars grafted on invigorati ...200616203719
combined heat and controlled atmosphere quarantine treatments for control of codling moth in sweet cherries.nonchemical quarantine treatments, using a combination of short-duration high temperatures under low oxygen, elevated carbon dioxide atmospheric environment were developed to control codling moth in sweet cherries, prunus avium (l.). the two treatments developed are a chamber temperature of 45 degrees c for 45 min and a chamber temperature of 47 degreesc for 25 min under a 1% oxygen, 15% carbon dioxide, -2 degrees c dew point environment. both these treatments have been shown to provide control ...200516022297
chemical constituents and antioxidant activity of sweet cherry at different ripening stages.the development and ripening process of sweet cherry (prunus avium l. cv. 4-70) on the tree was evaluated. for this purpose, 14 different stages were selected in accordance with homogeneous size and color. some parameters related to fruit quality, such as color, texture, sugars, organic acids, total antioxidant activity, total phenolic compounds, anthocyanins, and ascorbic acid were analyzed. the results revealed that in sweet cherry, the changes in skin color, glucose and fructose accumulation, ...200515796619
determining self-incompatibility genotypes in belgian wild cherries.abstract the self-incompatibility (s) genotypes of a collection of 65 belgian accessions of wild cherry, selected within two populations and planted in a seed orchard, were determined using polymerase chain reaction (pcr) methods. initially, dna extracts were amplified with consensus primers that amplify across the second intron of the s-ribonuclease gene which shows considerable length polymorphism. the provisional genotypes deduced were checked with the appropriate allele-specific primers for ...200515773927
loss of pollen-s function in two self-compatible selections of prunus avium is associated with deletion/mutation of an s haplotype-specific f-box gene.recently, an s haplotype-specific f-box (sfb) gene has been proposed as a candidate for the pollen-s specificity gene of rnase-mediated gametophytic self-incompatibility in prunus (rosaceae). we have examined two pollen-part mutant haplotypes of sweet cherry (prunus avium). both were found to retain the s-rnase, which determines stylar specificity, but one (s3' in ji 2434) has a deletion including the haplotype-specific sfb gene, and the other (s4' in ji 2420) has a frame-shift mutation of the h ...200515598801
expression of a sweet cherry dreb1/cbf ortholog in arabidopsis confers salt and freezing tolerance.dehydration responsive element binding protein 1 (dreb1)/c-repeat binding factor (cbf) induces the expression of many stress-inducible genes in arabidopsis. we have previously reported the identification of three dreb1/icbf homologs from sweet cherry (prunus avium). to identify the function of these homologs, one of the genes, cig-b, was transformed into arabidopsis. in one of the transgenic plant lines, the dreb1/cbf target gene cor15a was induced in the absence of stress treatment. the cor15a- ...200415535126
the distribution of little cherry disease in germany: lchv1 and lchv2 in cultivars of sweet cherry and in some other species. 200112425030
genetic relationships between diploid and allotetraploid cherry species (prunus avium, prunus x gondouinii and prunus cerasus).prunus avium l. (diploid, aa, 2n=2x=16), prunus cerasus l. (allotetraploid, aaff, 2n=4x=32) species, and their hybrid prunus x gondouinii rehd., constitute the most widely cultivated cherry tree species. p. cerasus is supposed to be an hybrid species produced by the union of unreduced p. avium gametes and normal p. fruticosa gametes. a continuum of morphological traits between these three species makes their assignation difficult. the aim of this paper is to study the genetic relationships betwe ...200415354194
the role of polar auxin transport through pedicels of prunus avium l. in relation to fruit development and retention.it was investigated whether premature fruit abscission in prunus avium l. was triggered by a reduction in polar auxin transport (pat). the capacity of pedicels to transport tritiated iaa ([3h]-iaa) via the pat pathway was measured at intervals throughout flower and fruit development. the extent of passive diffusion, assessed by concurrent applications of [14c]-benzoic acid ([14c]-ba), was negligible. transported radioactivity recovered from agar blocks eluted at the same retention time as authen ...200415310825
two-dimensional tension tests in plant biomechanics--sweet cherry fruit skin as a model system.splitting of fruits is a function of two-dimensional tension caused by different growth rates of tissues and turgor, especially water uptake shortly before harvest. in order to analyse the mechanical properties of spheroid plant material close to stress-strain conditions in vivo, a new hydraulic two-dimensional testing device was set up. sweet cherry (prunus avium l.) fruit skin was chosen as a model system. the recorded pressure-deflection curves were non-linear, with a considerable initial "la ...200415248126
hyperhydricity of prunus avium shoots cultured on gelrite: a controlled stress response.hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. hyperhydric shoots are characterised by a translucent aspect due to a chlorophyll deficiency, a not very developed cell wall and a high water content. hyperhydricity of prunus avium shoots was expressed in vitro in one multiplication cycle by replacing the gelling agent agar (normal shoots: ns) by gelrite (hyperhydric shoots: hs). p. avium shoots evolving towards the hyperhydric state produce ...200415246065
maillard reaction and enzymatic browning affect the allergenicity of pru av 1, the major allergen from cherry (prunus avium).the influence of thermal processing and nonenymatic as well as polyphenoloxidase-catalyzed browning reaction on the allergenicity of the major cherry allergen pru av 1 was investigated. after thermal treatment of the recombinant protein rpru av 1 in the absence or presence of carbohydrates, sds-page, enzyme allergosorbent tests, and inhibition assays revealed that thermal treatment of rpru av 1 alone did not show any influence on the ige-binding activity of the protein at least for 30 min, thus ...200415186129
effect of temperature on pollen tube kinetics and dynamics in sweet cherry, prunus avium (rosaceae).prevailing ambient temperature during the reproductive phase is one of several important factors for seed and fruit set in different plant species, and its consequences on reproductive success may increase with global warming. the effect of temperature on pollen performance was evaluated in sweet cherry (prunus avium l.), comparing as pollen donors two cultivars that differ in their adaptation to temperature. 'sunburst' is a cultivar that originated in canada with a pedigree of cultivars from no ...200421653411
changes in strain and deposition of cuticle in developing sweet cherry fruit.changes in surface area, deposition and elastic strain of the cuticular membrane (cm) were monitored during development of sweet cherry (prunus avium l.) fruit. fruit mass and surface area ('sam') increased in a sigmoidal pattern between 16 and 85 days after full bloom (dafb) with maximum rates of 0.35 g day(-1) and 0.62 cm(2) day(-1), respectively. rates of total area strain, namely the sum of elastic plus plastic strain, were highest in cheek and stem cavity regions followed by stylar and sutu ...200415032829
effect of ripeness and postharvest storage on the phenolic profiles of cherries (prunus avium l.).the phenolic compounds hydroxycinnamates, anthocyanins, flavonols, and flavan-3-ols of sweet cherry cultivars burlat, saco, summit, and van harvested in 2001 and 2002 were quantified by hplc-dad. phenolics were analyzed at partially ripe and ripe stages and during storage at 15 +/- 5 degrees c (room temperature) and 1-2 degrees c (cool temperature). neochlorogenic and p-coumaroylquinic acids were the main hydroxycinnamic acid derivatives, but chlorogenic acid was also identified in all cultivars ...200414759143
studies on water transport through the sweet cherry fruit surface. 11. fecl3 decreases water permeability of polar pathways.the effect of fecl3 (10 mm) on osmotic water uptake into detached sweet cherry fruit (prunus avium l.) and on the (3)h2o permeability (p(d)) of excised exocarp segments (es) or enzymatically isolated cuticular membranes (cm) was investigated. es or cm were mounted in an infinite dose diffusion system, where diffusion is monitored from a dilute donor solution through an interfacing es or cm into a receiver solution under quasi steady-state conditions. in the absence of fecl3, (3)h2o diffusion thr ...200616910722
identification of incompatibility alleles in the tetraploid species sour cherry.the incompatibility genetics of sour cherry ( prunus cerasus), an allotetraploid species thought to be derived from sweet cherry (diploid) and ground cherry (tetraploid), were investigated by test crossing and by analysis of stylar ribonucleases which are known to be the products of incompatibility alleles in sweet cherry. stylar extracts of 36 accessions of sour cherry were separated electrophoretically and stained for ribonuclease activity. the zymograms of most accessions showed three bands, ...200414689184
studies on water transport through the sweet cherry fruit surface. 10. evidence for polar pathways across the exocarp.water uptake through the fruit surface is considered as an important factor in cracking of sweet cherry (prunus avium l.) fruit. uptake may occur by diffusion and/or viscous flow along a polar pathway. to establish the mechanism of water uptake, the effects of viscosity and molecular weight of selected osmotica on water uptake into detached sweet cherry fruit were investigated. in addition we investigated the effect of temperature on penetration of 2-(1-naphthyl)[1-(14)c]acetic acid ([(14)c]naa; ...200616719520
variation in the composition of the heartwood flavonoids of prunus avium by on-column capillary gas chromatography.a fast and simple extraction procedure, coupled with on-column gas chromatography, has been developed in order to investigate the relative amounts of flavonoids in individual trees within several homogeneous populations of prunus avium. eight known flavonoid aglycones were isolated from the heartwood of p. avium and multivariate analysis was employed in order to characterise population groups.200614667064
a cdna encoding a cold-induced glycine-rich rna binding protein from prunus avium expressed in embryonic axes.a cdna clone encoding a presumed full-length glycine-rich ribonucleic acid (rna) binding protein was isolated from a lambda-zap express cdna library generated from primarily nondormant prunus avium (wild cherry) embryonic axes. the cdna, designated pa-rrm-grp1 (prunus avium rna recognition motif glycine-rich protein 1), contains a single n-terminal rna recognition motif (rrm) and single c-terminal glycine-rich domain. the glycine-rich domain is unusually long at 91 amino acids, 58 of which are g ...200314597401
cryopreservation of prunus avium l. embryogenic tissues.embryogenic tissues from wild cherry (prunus avium l.) were successfully cryopreserved by using a one-step freezing procedure. cryoprotection consisted of a pretreatment on solid medium with increasing sucrose concentrations (0.25 m for 1 day, 0.5 m for 1 day, 0.75 m for 2 days, and 1.0 m for 3 days), followed by air desiccation to about 20 percent moisture content (fresh weight basis). this method was compared with a pretreatment on solid medium containing 5 percent dmso and 2 percent proline, ...201316547548
translocation of nitrogen in the xylem of field-grown cherry and poplar trees during remobilization.studies of small trees growing in pots have established that individual amino acids or amides are translocated in the xylem sap of a range of tree species following bud burst, as a consequence of nitrogen (n) remobilization from storage. this paper reports the first study of n translocation in the xylem of large, deciduous, field-grown trees during n remobilization in the spring. we applied 15n fertilizer to the soil around 10-year-old prunus avium l. and populus trichocharpa torr. & gray ex hoo ...200616414931
a pollen-expressed gene for a novel protein with an f-box motif that is very tightly linked to a gene for s-rnase in two species of cherry, prunus cerasus and p. avium.this study describes a novel f-box protein gene in the s-locus of sour cherry (prunus cerasus) and sweet cherry (p. avium). the gene showed an s-haplotype-specific sequence polymorphism and the expression was specific to pollen. genomic dna blot analysis of eight sweet cherry cultivars with the probe for the f-box protein gene under low stringency conditions yielded rflp bands specific to the s-haplotypes of each cultivar. we discuss the possibility of the gene for the f-box protein being a cand ...200312881505
characterization of the s-rnase promoters from sweet cherry (prunus avium l.).genomic dna fragments containing the s(3)-, s(4)-, and s(6)-rnase genes were isolated from the sweet cherry (prunus avium l.) and sequenced. comparison of the 5'-flanking sequences of these three s-rnases indicated that a highly conserved region (designated cr) existed just upstream from the putative tata boxes. we postulate that cr contains cis-regulatory element(s) involved in pistil expression. to examine the activity of the isolated s-rnase promoters of sweet cherry in the pistil, we transie ...200312773819
identification of a non-s rnase, a possible ancestral form of s-rnases, in prunus.this study identifies and characterizes a basic non-s rnase in the styles with stigmas of sweet cherry (prunus avium l.), a member of the rosaceae subfamily amygdaloideae, which has an rnase-based gametophytic self-incompatibility system. internal sequences of putative non-s rnases (rnase pa1 and pa2) were determined, and a cdna for pa1 was obtained. the deduced amino acid sequence of pa1 contained two conserved sequence motifs essential for t2/ s-type rnase activity. pa1 shows 20-30% sequence i ...200312715157
characterization of microsatellites in wild and sweet cherry (prunus avium l.)--markers for individual identification and reproductive processes.nuclear microsatellites were characterized in prunus avium and validated as markers for individual and cultivar identification, as well as for studies of pollen- and seed-mediated gene flow. we used 20 primer pairs from a simple sequence repeat (ssr) library of prunus persica and identified 7 loci harboring polymorphic microsatellite sequences in p. avium. in a natural population of 75 wild cherry trees, the number of alleles per locus ranged from 4 to 9 and expected heterozygosity from 0.39 to ...200312669801
differences in root longevity of some tree species.although the importance of root production and mortality to nutrient fluxes in ecosystems is widely recognized, the difficulties associated with root measurements have limited the availability of reliable data. we have used minirhizotrons and image analysis to measure root longevity of prunus avium l., picea sitchensis (bong.) carrière, acer pseudoplatanus l. and populus x canadensis cv. beaupre directly in cohorts of roots. major differences in the longevity of roots among species were identifi ...199812651380
air pollutants from hydrocarbons and derivatives in micropropagation laboratories: toxicity symptoms on tissue culture of the cherry rootstock colt (prunus avium x p. pseudocerasus).several air pollutants in research and micropropagation laboratories originate from the combustion of hydrocarbons and their derivatives. the combustion products of some natural gases (propane-butane, propane, methane) and ethanol were analyzed, and the atmosphere composition was investigated inside the laminar flow box, inside the room where transplanting is performed and inside the culture vessels after transplanting. large quantities of ethylene and other biologically active compounds are pro ...199024227058
somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (pyrus communis var. pyraster l.) and colt cherry (prunus avium x pseudocerasus).mesophyll protoplasts of wild pear (pyrus communis var. pyraster l., pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock colt (prunus avium x pseudocerasus, prunoideae), following an electroporation treatment of the separate parental protoplast systems. fusion-treated protoplasts were cultured, on modified k8p medium, where it had been previously established that neither parental protoplasts were capable of division. somatic hybrid calli were recovered and, foll ...198924227027
studies on water transport through the sweet cherry fruit surface. 7. fe3+ and al3+ reduce conductance for water uptake.the effects of the chloride salts licl, cacl(2), mgcl(2), alcl(3), eucl(3), and fecl(3) and the iron salts fecl(2), fecl(3), fe(no(3))(3), feso(4), and fe(2)(so(4))(3) on water conductance of exocarp segments (es) and rates of water uptake into detached sweet cherry fruit (prunus avium l. cv. adriana, early rivers, namare, namosa, and sam) were studied. es were excised from the cheek of mature fruit and mounted in stainless steel diffusion cell; water penetration was monitored gravimetrically fr ...200212475277
molecular characterisation of sweet cherry (prunus avium l.) genotypes using peach [prunus persica (l.) batsch] ssr sequences.a total of 76 sweet cherry genotypes were screened with 34 microsatellite primer pairs previously developed in peach. amplification of ssr loci was obtained for 24 of the microsatellite primer pairs, and 14 of them produced polymorphic amplification patterns. on the basis of polymorphism and quality of amplification, a set of nine primer pairs and the resulting 27 informative alleles were used to identify 72 genotype profiles. of these, 68 correspond to unique cultivar genotypes, and the remaini ...200212080370
studies on water transport through the sweet cherry fruit surface: iii. conductance of the cuticle in relation to fruit size.rain-cracking of sweet cherry fruit has been related to water absorption through the fruit surface and large fruit has been reported to be more susceptible to cracking than small fruit. therefore, the effect of fruit size on water conductance of the cuticular membrane (cm) of exocarp segments excised from cheek, suture or stylar end region of mature sweet cherry fruit (prunus avium l. cv. sam) was investigated. segments consisting of epidermis, hypodermis and several layers of mesocarp cells wer ...200212060264
inhibition of growth and xylogenesis and promotion of vacuolation in prunus callus by the flavanone prunin.callus tissue of prunus avium l. responded to supplied prunin (naringen in 7-glucoside) showing vaculoation and storage of oligomeric proanthocyanidins. in addition, prunin caused restricted callus initiation and/or less callus growth. when prunin was omitted from the medium numerous tracheids and more peroxidases were formed in the callus.198824241596
evolutionary analysis of s-rnase genes from rosaceae species.eight new cdna sequences for s-rnases were cloned and analysed from almond (prunus dulcis) cultivars of european origin, and compared to published sequences from other rosaceae species. insertions/deletions of 10-20 amino acid residues were detected in the rc4 and c5 domains of s-rnases from almond and sweet cherry. the s-rnases of the prunus species and those of the genera malus and pyrus formed two distinct groups on phylogenetic analysis. nucleotide substitutions were analysed in the s-rnase ...200211919717
radiocaesium contamination of meadow vegetation--time-dependent variability and influence of soil characteristics at grassland sites in austria.long-term trends of 137cs and 40k concentrations in meadow grass and soil-plant transfer data at eight different sites in upper austria are presented. geometric means of 137cs tf-data and tagg values vary between 0.03-1.06 and 0.0005-0.0184 depending on site, respectively. 40k results are less variable with tf values covering a range of 0.31-2.01. only at one site was a significant decrease of 137cs concentration (decay-corrected) in meadow vegetation observed during the observation period 1992- ...200211814163
a population genetic analysis of chloroplast dna in wild populations of prunus avium l. in europe.a population genetic study of chloroplast dna was carried out in 23 wild populations of prunus avium sampled from several european deciduous forests. an analysis of approx. 9% of the chloroplast genome detected mostly insertion-deletion mutations and one point mutation. in all, 16 haplotypes were detected. six haplotypes were shared by two or more populations and 10 were unique. one haplotype was present in 21 of the 23 populations and 161 of 211 individuals, which probably indicates its ancient ...200111737289
style antigens of prunus avium l.antiserum to a protein fraction of an extract of mature styles of p. avium cv. lambert (s 3 s 4) was raised in rabbits. two major antigenic components of the style extracts were detected by immunoelectrophoresis and immunodiffusion. the presence of one antigen (s-antigen) correlated with a particular s-genotype (s 3 s 4). this antigen is restricted to mature styles of p. avium. the second antigen (p-antigen) was detected in styles of all prunus species examined, but not in styles of other specie ...198124276762
studies on water transport through the sweet cherry fruit surface: ii. conductance of the cuticle in relation to fruit development.water conductance of the cuticular membrane (cm) of sweet cherry (prunus avium l. cv. sam) fruit during stages ii and iii (31-78 days after full bloom, dafb) was investigated by gravimetrically monitoring water loss through segments of the exocarp. segments were mounted in stainless-steel diffusion cells, filled with 0.5 ml of deionized water and incubated for 8 h at 25 +/- 2 degrees c over dry silica. conductance was calculated by dividing the amount of water transpired per unit surface area an ...200111722129
cytological zonation of prunus avium l. pollen tubes in vivo. 19807381990
fluorine content of vegetables and fruits.twenty-six species of vegetables grown in szeged, in its environment and at szabadkigyós were examined for their f content. the mean f content of the most important staple products is 0.30 mg/kg raw weight or less. when vegetables of the same kind were examined, the results were mostly different. the highest mean f content was obtained for spinach (0.55 mg); potato yielded less (0.15 mg), and the lowest was obtained for cucumber (0.06 mg). the mean f content of the 13 fruit species was 0.10 mg/k ...1978754476
ultrastructural localization of acid phosphatase in the pollen tube of prunus avium l. (sweet cherry).the pollen tube of prunus avium (cherry) consists of a growth zone of vesicles at the tip and an assemblage of organelles typical of an actively metabolizing cell. electron opaque globules are closely associated with the plasma membrane and fibrillar cell wall layer at the tip. acid phosphatase (ec 3.1.3.2) activity is localized in the membranes of 120 nm vesicles and er system, the lumen of 50 nm vesicles, the plasma membrane and the tube nucleus.197724420022
[acquisition and study of callus tissue during cultivation of mazzard and strawberry anthers].anters of 7 varieties of cerasus avium (2n=16) and fragaria grandiflora (2n==56) were cultured on murashige-skoog's medium. formation of the callus tissue was observed for only one variety of sweat cherry with a frequency of 3.1-20.6%. the number of haploid cells in first transfers varied from 22 to 44% in different strains. there were two rises of mitotic activity in the callus cells. subsequent transfers caused an increase in haploid cells. chromosome aberrations frequency was comparatively lo ...20081025784
inhibition by carboxylic acids of an o-diphenol oxidase from prunus avium fruits. 19744208079
[the induction of an abscission layer in fruit of prunus avium l. by 2-chloroethylphosphonic acid].2-chloroethylphosphonic acid (cepa) facilitates the separation of the fruit from the pedicel significantly. the application of 2,000 and 4,000 ppm cepa in four sweet cherry varieties during maturation resulted in the formation of a complete abscission layer in the transition zone between pedicel and fruit. in contrast, in the untreated fruit no abscission layer was evident at maturity. the walls of the cells in the abscission layer contained less total polysaccharides than adjacent cells. cellul ...197024499842
[biogenesis of plant pigments. ii. on the biogenesis of flavanones and flavanonols of robinia pseudoacacia, prunus avium and prunus mahaleb]. 19676056735
are hyperhydric shoots of prunus avium l. energy deficient?the content of oxidized and reduced pyridine nucleotides and some enzymatic activities of the oxidative pentose phosphate and glycolytic pathways were compared in normal (ns, growing on agar) and hyperhydric (hs, growing on gelrite) shoots of prunus avium l. after 4 weeks of in vitro culture. the chlorophyll fluorescence from leaves and the redox capacity of the plasma membrane (reduction of exogenously added ferricyanide) of both types of shoots were recorded. the pool of oxidized and reduced p ...200111337071
purification and characterisation of a beta-glucosidase abundantly expressed in ripe sweet cherry (prunus avium l.) fruit.a beta-glucosidase (beta-d-glucoside glucohydrolase, ec 3.2.1.21) was purified to homogeneity from ripe fruits of sweet cherry (prunus avium l.) by ammonium sulphate precipitation, ion exchange and size exclusion chromatography. the enzyme is a monomer with a molecular mass of approximately 68 kda and an acidic isoelectric point. n-terminal sequence analysis indicated that sweet cherry beta-glucosidase is related to other plant cyanogenic beta-glucosidases. substrate specificity studies revealed ...200111297776
studies on water transport through the sweet cherry fruit surface: characterizing conductance of the cuticular membrane using pericarp segments.water conductance of the cuticular membrane (cm) of mature sweet cherry fruit (prunus avium l. cv. sam) was investigated by monitoring water loss from segments of the outer pericarp excised from the cheek of the fruit. segments consisted of epidermis, hypodermis and several cell layers of the mesocarp. segments were mounted in stainless-steel diffusion cells with the mesocarp surface in contact with water, while the outer cuticular surface was exposed to dry silica (22 +/- 1 degrees c). conducta ...200011219577
physicochemical characteristics of selected sweet cherry cultivars.the volatile, sugar, and organic acid constituents in 12 cultivars and selections of sweet cherries (prunus avium l.) were characterized and quantified by high-performance liquid chromatography and gas chromatography (gc). fruit weight, soluble solids concentration (ssc), ph, titratable acidity (ta), and color (cie l, a, b) were also determined at harvest. weight ranged from 8.8 to 14.5 g per fruit, ssc from 13.5 to 24.5 degrees brix, and ssc/ta ratio from 18.3 to 29.0. chroma was a better indic ...199810554265
[flavone compounds of wood extracts. v. water-soluble derivatives of sweet cherry heart (prunus avium)]. 195713437004
[relation between the calcium and mineral phosphate content of the soil and the content of crude and elaborated sap of the wild cherry tree (prunus avium)]. 195513260867
[biochemistry of the sap of the wild cherry prunus avium]. 195513240336
[indeterminate chemistry of wood extracts. iv. new flavone derivatives from the heart of the wild cherry tree (prunus avium)]. 195413219528
tatter leaf of sweet cherry. 194621015648
the perfect stage of cylindosporium on prunus avium. 191317793207
chloroplast inheritance and dna variation in sweet, sour, and ground cherry.sour cherry (prunus cerasus l.) is an allotetraploid and both sweet cherry (p avium l.) and ground cherry (p. fruticosa pall.) are the proposed progenitor species. the study investigated the maternal species origin(s) of sour cherry using chloroplast dna (cpdna) markers and a diverse set of 22 sweet, 25 sour, and 7 ground cherry selections. two cpdna restriction fragment length polymorphisms (rflps) and one polymerase chain reaction (pcr) fragment length polymorphism were identified among the 54 ...200810739133
direct observations of the redox states of frozen cherry buds by a unique in vivo esr.low temperatures cause cellular damage in flower buds of the sweet cherry (prunus avium l. cv. satohnishiki). in this study, the redox states within the cherry buds suffering freezing damage were non-destructively observed by a unique in vivo electron spin resonance (esr) technique with a spin probe such as carbamoyl-proxyl. the esr signals of carbamoyl-proxyl-treated bud were continuously recorded under freezing and thawing condition, which was decreased to approximately -4 degrees c and mainta ...200314511650
recycling of nitrogen in the xylem of prunus avium trees starts when spring remobilization of internal reserves declines.nitrogen (n) storage capacity of cherry (prunus avium l.) trees grown in sand culture was preconditioned by applying contrasting n supplies for one year. during the spring of the following year, a constant amount of 15n was supplied and the dynamics of n remobilization and root uptake were characterized as a function of internal n status of the trees. to calculate the flux of n through xylem, both xylem sap n concentration and whole-tree transpiration rates were measured. by comparing the cumula ...200312975130
cloning and characterization of genomic dna sequences of four self-incompatibility alleles in sweet cherry ( prunus avium l.).gametophytic self-incompatibility (gsi) in sweet cherry is determined by a locus s with multiple alleles. in the style, the s-locus codifies for an allele-specific ribonuclease ( s-rnase) that is involved in the rejection of pollen that carries the same s allele. in this work we report the cloning and genomic dna sequence analysis including the 5' flanking regions of four s-rnases of sweet cherry ( prunus avium l., rosaceae). dna from the cultivars ferrovia, pico colorado, taleguera brillante an ...200412955210
biochemical characterization of pru a 2, a 23-kd thaumatin-like protein representing a potential major allergen in cherry (prunus avium).the prevalence of allergy to fruits and vegetables increased with pollinosis over the last 10 years. so far, clusters of hypersensitivity have been established and corroborated by the molecular characterization of individual cross-reacting allergens. several case studies demonstrated the existence of allergic reactions to fruits of the subfamily prunoideae (apricots, cherries, plums and peaches). here, we present the characterization of a major allergen in cherry.19989623505
cytological comparison of leaves and stems of prunus avium l. shoots cultured on a solid medium with agar or gelrite.an axillary proliferating clone of prunus avium l. was subcultured every four weeks on solid ms medium with agar as the gelling agent. vitrification (hyperhydricity) of shoots was induced in one four week cycle with the same medium except that agar was replaced by gelrite. during culture on the vitrifying medium, the water content of the shoots progressively increased with a parallel decrease in chlorophyll content. cytological differences between the leaves and stems of the vitrified and normal ...19989554582
effects of elevated co(2) on chloroplast components, gas exchange and growth of oak and cherry.specific chloroplast proteins, gas exchange and dry matter production in oak (quercus robur l.) seedlings and clonal cherry (prunus avium l. x pseudocerasus lind.) plants were measured during 19 months of growth in climate-controlled greenhouses at ambient (350 vpm) or elevated (700 vpm) co(2). in both species, the elevated co(2) treatment increased the ppfd saturated-rate of photosynthesis and dry matter production. after two months at elevated co(2), prunus plants showed significant increases ...199714759855
inheritance and linkage of isozymes in sweet cherry (prunus avium l.).eight polymorphic isozyme loci, 6pgd, g6pd, mdh, pgm, skdh, fdp, got and idh, in sweet cherry where found to be in one linkage group, with a ninth isozyme locus, gpi, being in another linkage group on a different chromosome. isozymes were also linked to the incompatibility s locus and this explained the disturbed segregation ratios observed in the first generation from controlled hybridisations between different sweet cherry cultivars. analysis revealed close linkage between the isozyme and s lo ...199624162301
a cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like.a 29-kd polypeptide is the most abundant soluble protein in ripe cherry fruit (prunus avium l); accumulation begins at the onset of ripening as the fruit turns from yellow to red. this protein was extracted from ripe cherries and purified by size-exclusion and ion-exchange chromatography. antibodies to the purified protein were used to screen a cdna library from ripe cherries. numerous recombinant plaques reacted positively with the antibodies; the dna sequence of representative clones encoded a ...19968685266
a linkage map of sweet cherry based on rapd analysis of a microspore-derived callus culture population.a partial linkage map was constructed for the sweet cherry (prunus avium l.) cultivar emperor francis from a population of 56 microspore-derived callus culture individuals. the callus cultures were genotyped for two allozymes and 90 random amplified polymorphic dna (rapd) markers using 79 random decanucleotide dna primers and the polymerase chain reaction (pcr). eighty-nine markers mapped to 10 linkage groups totaling 503.3 cm. dna blot and hybridization analysis using five cloned rapds as probe ...20108683097
isozyme diversity in sour, sweet, and ground cherry.thirty-six sour (prunus cerasus l.), sweet (p. avium l.), and ground cherry (p. fruticosa pall.) selections were evaluated for seven enzyme systems and principal coordinate analysis was used to examine isozyme divergence among these cherry species. the enzyme systems studied were phosphoglucose isomerase (pgi), isocitrate dehydrogenase (idh), phosphoglucomutase (pgm), 6-phosphogluconate dehydrogenase (6-pgd), leucine aminopeptidase (lap), shikimate dehydrogenase (skdh), and malate dehydrogenase ...199524172928
sweet cherry cultivar identification by leaf isozyme polymorphism.isozyme polymorphism can assist in the identification of cherry cultivars. ten isozymes, each showing variation at only one locus, provide 70 unique genotype profiles from leaf extracts of 78 different sweet cherry cultivars. polymorphism in 6pgd, g6pd, gpi, idh, pgm, fdp, skdh and per is demonstrated for the first time, while observations are extended for the previously described polymorphisms in mdh and got. some cultivars with identical morphological characters and previously treated as one c ...199324193593
localization and quantitative determination of catechins and proanthocyanidins in the phloem of elm and cherry.the localization of catechins and proanthocyanidins in the phloem of elm (ulmus minor mill.) and cherry (prunus avium l.) was determined histochemically by use of 4-dimethylaminocinnamaldehyde (dmaca) reagent. the two tree species showed a characteristic distribution of these phenols, which were most abundant in the phloem rays of cherry, but were largely confined to upright parenchyma cells of elm. quantitative determination and qualitative separation of catechins and proanthocyanidins were per ...199214969867
cell wall synthesis and salt (saline) sensitivity in cultured colt cherry (prunus avium × pseudocerasus) protoplasts.in order to investigate the cellular basis of salt tolerance, colt cherry (prunus avium ×pseudocerasus) protoplasts from mesophyll tissues and root cell suspension cultures were cultured in the presence of nacl, kcl or na2so4, at normalities of 25, 50, 100 or 200 mn for each salt and with or without 2,6-dichlorobenzonitrile, an inhibitor of cell wall synthesis. results showed that the acquisition of salt tolerance was concomitant with the onset of cell wall regeneration, with protoplasts exhibit ...198924233277
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