TitleAbstractYear(sorted descending)
a novel biomarker for marine environmental pollution of hsp90 from mytilus coruscus.heat shock protein 90 (hsp90) is a conserved molecular chaperone contributing to cell cycle control, organism development and the proper regulation of cytosolic proteins. the full-length hsp90 cdna of mytilus coruscus (mchsp90, kt946644) was 2420bp, including an orf of 2169bp encoding a polypeptide of 722 amino acids with predicted pi/mw 4.89/83.22kda. blastp analysis and phylogenetic relationship strongly suggested mchsp90 was a member of hsp90 family, and it was highly conserved with other kno ...201627491367
expression, purification and initial characterization of a novel recombinant antimicrobial peptide mytichitin-a in pichia pastoris.mytichitin-a is an antimicrobial peptide isolated from the serum of mytilus coruscus and is reported to inhibit bacterial growth as tested on several gram-positive bacteria. to produce large quantity of mytichitin-a to further investigate its biological activity, nucleotide sequence encoding a recombinant 6 × his-mytichitin-a (rmytichitin-a) peptide was synthesized and inserted into the inducible yeast expression vector ppiczαa. with the availability of such an expression vector called ppiczαa-m ...201627389469
wnt7b gene expression and functional analysis in the mussel mytilus understand the potential functions of wnt7b in different developmental stages and adult tissues of the mussel mytilus coruscus, the wnt7b gene was cloned using the rapid amplification of cdna ends technique. the full-length wnt7b gene was 1910 bp long, with a 1059-bp open reading frame encoding 352 amino acids. the amino acid sequence of the m. coruscus wnt7b gene shared high homology with that of homo sapiens (59%), mus musculus (61%), danio rerio (61% similarity), biomphalaria glabrata (62% ...201627819717
lipophilic shellfish toxins in dinophysis caudata picked cells and in shellfish from the east china sea.we reported previously that okadaic acid (oa) and dinophysistoxin-1 (dtx1) were responsible for diarrhetic shellfish poisoning (dsp) incidents due to consuming cultivated mussels (mytilus galloprovincialis) in coastal cities near the east china sea in may 2011. pectenotoxin-2 (ptx2) and its seco acids were also present in these mussels. causative species of microalgae were not identified because detailed information on the location of the contaminated shellfish was not recorded. in order to expl ...201525233922
draft genome sequence of shewanella sp. ecsmb14102, a mussel recruitment-promoting bacterium isolated from the east china sea.shewanella sp. ecsmb14102, which promotes recruitment of the mussel mytilus coruscus, was isolated from natural biofilms formed on glass slides submerged in the east china sea. here, we present the draft genome sequence, which comprises 4.41 mb with a g+c content of 52.2%. the genomic information in this strain will contribute to deepening our understanding of bacteria-animal interaction.201526089429
a novel biomarker for marine environmental pollution of pi-class glutathione s-transferase from mytilus coruscus.glutathione s-transferases (gsts) are the superfamily of phase ii detoxification enzymes that play crucial roles in innate immunity. in this study, a pi-class gst homolog was identified from mytilus coruscus (named as mcgst1, kc525103). the full-length cdna sequence of mcgst1 was 621bp with a 5' untranslated region (utr) of 70bp and a 3'-utr of 201bp. the deduced amino acid sequence was 206 residues in length with theoretical pi/mw of 5.60/23.72kda, containing the conserved g-site and diversifor ...201525910687
a novel c1q-domain-containing (c1qdc) protein from mytilus coruscus with the transcriptional analysis against marine pathogens and heavy metals.the c1q-domain-containing (c1qdc) proteins, which are involved in various processes of vertebrates, are important pattern recognition receptors in innate immunity of invertebrates. in present study, a novel c1qdc was identified from mytilus coruscus (designated as mcc1qdc), which was 917 bp in length encoding 236 amino acids with a typical signal peptide of 19 amino acid residues in n-terminus. based on its conserved c1q domain and molecular architecture of 10 β-strand jelly-roll folding topolog ...201424296435
differential hsp70 expression in mytilus coruscus under various stressors.heat shock proteins (hsps) play crucial roles in protecting cells against environmental stresses, such as heat shock, heavy metals and pathogenic bacteria. among the hsp family, the 70-kda hsps are most responsible for intracellular chaperone and extracellular immunoregulatory functions. the full-length hsp70 cdna of mytilus coruscus (designated as mchsp70, genbank accession no. kf322135) was obtained, which was 2319 bp, including an orf of 1965 bp to encode a polypeptide of 655 amino acid with ...201424704274
aquimarina gracilis sp. nov., isolated from the gut microflora of a mussel, mytilus coruscus, and emended description of aquimarina orange-coloured and slender rod-shaped bacterium, designated strain psc32(t), was isolated from the gut microflora of a mussel collected from gwangyang bay, south sea (republic of korea). cells were gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. the major fatty acids were iso-c15 : 0, iso-c17 : 0 3-oh, iso-c15 : 1 g and iso-c15 : 0 3-oh. the only isoprenoid quinone of strain psc32(t) was mk-6 and the dna g+c content was 36.9 mol%. phosphatidylethanolamine, one u ...201322941302
larval settlement and metamorphosis of the mussel mytilus coruscus in response to monospecific bacterial biofilms.the effects of bacterial biofilms (bfs) on larval settlement and metamorphosis of the mussel, mytilus coruscus, were investigated in the laboratory. of nine different isolates, shewanella sp.1 bf induced the highest percentage of larval settlement and metamorphosis, whereas seven other isolates had a moderate inducing activity and one isolate, pseudoalteromonas sp. 4, had a no inducing activity. the inducing activity of individual bacterial isolates was not correlated either with their phylogene ...201323452123
molecular characterization of a novel antimicrobial peptide from mytilus coruscus.antimicrobial peptides (amps) are components of the innate immune responses that form the first line of host defense against pathogens. marine mussels can produce a surprising abundance of cysteine-rich amps pertaining to the defensin, myticin, mytilin and mytimycin families, particularly in the circulating hemocytes. in the current study, we purified and characterized a novel cysteine-rich peptide with remarkable antibacterial activity from mytilus coruscus and designated with myticusin-1, a 10 ...201323247103
preparation of ace inhibitory peptides from mytilus coruscus hydrolysate using uniform design.the angiotensin-i-converting enzyme (ace) inhibitory peptides from mussel, mytilus coruscus, were investigated and the variable factors, protease concentration, hydrolysis time, ph, and temperature, were optimized using uniform design, a new statistical experimental method. the results proved that the hydrolysate of alkali proteases had high ace-inhibitory activity, especially the alkali protease e1. optimization by uniform design showed that the best hydrolysis conditions for preparation of ace ...201223484103
aquimarina mytili sp. nov., isolated from the gut microflora of mussel, mytilus orange-coloured and rod shaped gliding bacterium, designated strain psc33t was isolated from the gut microflora of mussel collected from the south sea (republic of korea). cells were gram-reaction-negative, strictly aerobic, catalase- and oxidase-positive. the major fatty acids were iso-c15:0, iso-c17:0 3-oh, iso-c15:1 g, c15:0 3-oh and iso-c17:1ω9c. the dna g+c content was 37.9 mol%. a phylogenetic tree based on 16s rrna gene sequences showed that strain kyw382t forms an evolutionary lineage ...201122003036
purification, cdna clone, and recombinant expression of foot protein-3 from mytilus coruscus.mussels mytilus coruscus can adhere to various solid surface in the presence of moisture. mussel foot protein-3 (mfp-3) has been suggested as the main adhesive protein in the plaques closest to the adhesion interface and been the focus of substantial biomaterials development research within the last decade. the byssal plaques of m. coruscus were accumulated and variants of a family known as mcofp3 (mytilus coruscus foot protein 3) were purified from acetic acid/urea extracts of plaques, with the ...201121787281
seasonal variation in nutrient composition of mytilus coruscus from china.seasonal variation in the nutrient composition of mytilus coruscus (thick shell mussel), cultivated in shengsi islands, zhejiang province, china, was investigated in this study. proximate composition, mineral and amino acid concentrations, as well as the compositions of the lipid and fatty acid of thick shell mussels were analyzed. proximate composition and mineral contents were seasonally varied significantly except for calcium (ca) and lead (pb). glycine was the predominant amino acid in musse ...201020553047
[designation, solid-phase synthesis and antimicrobial activity of mytilin derived peptides based on mytilin-1 from mytilus coruscus].as a key role in mussel defense system, mytilin is an important antibacterial peptide isolated from the mussel serum. the structural and functional researches on mytilin showed that the fragment connecting two beta-sheets in a stable beta-hairpin structure was probably required for antimicrobial activity. to elucidate the structural features and the antimicrobial activity of this fragment, we re-designed and synthesized two peptides corresponding to the main mimic structures of mytilin-1 from my ...201020575446
characterization and protection on acute liver injury of a polysaccharide mp-i from mytilus this study, we analyzed a water-soluble polysaccharide mp-i isolated from mytilus coruscus. mp-i was obtained by hot-water extraction, anion-exchange and gel-permeation chromatography. complete hydrolysis, periodate oxidation, methylation analysis, as well as fourier transform infrared spectroscopy (ftir) and nuclear magnetic resonance (nmr) spectroscopy were conducted to elucidate its structure. mp-i was subjected to investigate the protective effect on carbon tetrachloride (ccl(4)) induced ...200817965434
sculezonones a and b, two metabolites possessing a phenalenone skeleton from a marine-derived fungus penicillium species.two new metabolites possessing a phenalenone skeleton, sculezonones a (1) and b (2), were isolated from cultured broth of the fungus penicillium sp., which was separated from the okinawan marine bivalve mytilus coruscus, and the structures were elucidated by spectroscopic data.200010757733
coruscol a, a new metabolite from the marine-derived fungus penicillium species.a new metabolite possessing a 1,3-dioxane ring, coruscol a (1), was isolated from the mycelium of the fungus penicillium sp., which was separated from the okinawan marine bivalve mytilus coruscus, and the structure was elucidated by spectroscopic data.200010869231
interference of free fatty acids from the hepatopancreas of mussels with the mouse bioassay for shellfish toxins.determination of free fatty acids (ffa) in hepatopancreas of mussel, mytilus coruscus, contaminated by diarrhetic shellfish poisoning (dsp) toxins, was carried out by high-performance liquid chromatography-fluorometry as their 9-anthryldiazomethane derivatives. the ffa in the hepatopancreas of mussels during may 30-june 14 were 443-666 micrograms/g. a marked increase in ffa content was observed on june 20 (2197 micrograms/g hepatopancreas) and june 27 (6322 micrograms/g hepatopancreas). the prop ...19968784745
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