| a genoproteomic approach to detect peptide markers of bacterial respiratory pathogens. | rapid identification of respiratory pathogens may facilitate targeted antimicrobial therapy. direct identification of bacteria in bronchoalveolar lavage (bal) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is confounded by interfering substances. we describe a method to identify unique peptide markers of 5 gram-negative bacteria by liquid chromatography-tandem mass spectrometry (lc-ms/ms) for direct pathogen identification in bal. | 2017 | 28588123 |
| [analysis of the spectrum and resistance of pathogen causing sepsis in patients with severe acute pancreatitis]. | objective: to investigate the characteristics of spectrum and drug resistance of pathogens causing sepsis in patients with severe acute pancreatitis(sap). methods: the clinical data of 63 sap patients with sepsis admitted in department of hepatobiliary, people's liberation army general hospital from january 2014 to december 2015 were retrospectively studied. there were 47 males and 16 females, aged from 22 to 73 years, with an average age of (52±11)years. samples were collected mainly from: (1)p ... | 2017 | 28464580 |
| in vitro activity of cefepime/zidebactam (wck 5222) against gram-negative bacteria. | diazabicyclooctanes (dbos) inhibit class a, class c and some class d β-lactamases. a few also bind pbp2, conferring direct antibacterial activity and a β-lactamase-independent 'enhancer' effect, potentiating β-lactams targeting pbp3. we tested a novel dbo, zidebactam, combined with cefepime. | 2017 | 28158732 |
| using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities. | the scope of the study was to apply phenotype biolog microarray (pm) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. in the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. in the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria wer ... | 2017 | 28451946 |
| an assessment on the role of endophytic microbes in the therapeutic potential of fagonia indica. | natural products of animals, plants and microbes are potential source of important chemical compounds, with diverse applications including therapeutics. endophytic bacteria that are especially associated with medicinal plants presents a reservoir of therapeutic compounds. fagonia indica has been recently investigated by numerous researchers because of its striking therapeutic potential especially in cancer. it is also reported that endophytes play a vital role in the biosynthesis of various meta ... | 2017 | 28764775 |
| antibiotic susceptibility pattern of burkholderia cepacia complex and stenotrophomonas maltophilia: a 5-year analysis. | | 2017 | 28681835 |
| bacterial bloodstream infections in a tertiary infectious diseases hospital in northern vietnam: aetiology, drug resistance, and treatment outcome. | bloodstream infections (bsis) are associated with high morbidity and mortality worldwide. however their aetiology, antimicrobial susceptibilities and associated outcomes differ between developed and developing countries. systematic data from vietnam are scarce. here we present aetiologic data on bsi in adults admitted to a large tertiary referral hospital for infectious diseases in hanoi, vietnam. | 2017 | 28701159 |
| morphological analysis of biofilm of peritoneal dialysis catheter in refractory peritonitis patient. | a 66-year-old man undergoing peritoneal dialysis (pd) was admitted to our hospital for treatment of pd-related peritonitis. culture of the pd fluid revealed the presence of citrobacter freundii, and therapy with ceftazidime was started intraperitoneally. the cell count in pd fluid slowly decreased over time during the first 2 weeks of treatment, but increased again on the 14th hospital day. a second culture of the pd fluid revealed the presence of enterococcus species. a switch in antibiotic the ... | 2012 | 28509153 |
| imipenem-resistant gram-negative bacterial isolates carried by persons upon medical examination in korea. | carbapenem-resistant gram-negative bacteria (cr-gnb) have emerged and disseminated worldwide, become a great concern worldwide including korea. the prevalence of fecal carriage of imipenem-resistant gram-negative bacteria (ir-gnb) in persons in korea was investigated. stool samples were collected from 300 persons upon medical examination. samples were screened for ir-gnb by using macconkey agar with 2 μl/ml imipenem. species were identified by 16s rrna gene sequence analysis, and antimicrobial s ... | 2017 | 28721477 |
| yeasts harbored by vespine wasps in the pacific northwest. | the ecological role of social wasps has been extensively studied, but little is known about symbiotic relationships of these wasps with microbes. recently, it was shown that vespid wasps in europe carry yeasts, predominantly saccharomyces cerevisiae, in their gastrointestinal (gi) tract. interestingly, this niche allowed for sexual recombination of yeasts to occur and the formation of novel hybrid species. our goals were 1) to survey the gi tract of eusocial wasps in the pacific northwest for th ... | 2017 | 28169397 |
| enteric bacteria of food ice and their survival in alcoholic beverages and soft drinks. | this study aimed to evaluate the levels of enteric bacteria in ice cubes produced in different environments (home-made, prepared in bars and pubs with ice machines and produced in industrial plants) and to determine their survival in different alcoholic beverages and soft drinks. members of the enterobacteriaceae family were found in almost all samples analysed. all industrial and the majority of home-made samples did not contain coliforms. enterococci were not identified in domestic samples whi ... | 2017 | 28648289 |
| public health and healthcare-associated risk of electric, warm water bidet toilets. | in recent years, installation of bidet toilets within hospitals in japan has raised concerns regarding potential for cross-contamination by antimicrobial resistant bacteria from patients who are hospitalized over an extended period. | 2017 | 28756169 |
| activity of ceftolozane/tazobactam against surveillance and 'problem' enterobacteriaceae, pseudomonas aeruginosa and non-fermenters from the british isles. | we assessed the activity of ceftolozane/tazobactam against consecutive isolates collected in the bsac bacteraemia surveillance from 2011 to 2015 and against 'problem' isolates sent to the uk national reference laboratory from july 2015, when routine testing began. | 2017 | 28520867 |
| relationship of the crebc two-component regulatory system and inner membrane protein cred with swimming motility in stenotrophomonas maltophilia. | the crebc two-component system (tcs) is a conserved regulatory system found in escherichia coli, aeromonas spp., pseudomonas aeruginosa, and stenotrophomonas maltophilia. in this study, we determined how crebc tcs regulates secreted protease activities and swimming motility using creb, crec, and crebc in-frame deletion mutants (kjδcreb, kjδcrec, and kjδbc) of s. maltophilia kj. compared to wild-type kj, kjδcreb had a comparable secreted protease activity; however, the secreted protease activitie ... | 2017 | 28437463 |
| the effect of dietary bacterial organic selenium on growth performance, antioxidant capacity, and selenoproteins gene expression in broiler chickens. | selenium (se) is an essential trace mineral in broilers, which has several important roles in biological processes. organic forms of se are more efficient than inorganic forms and can be produced biologically via se microbial reduction. hence, the possibility of using se-enriched bacteria as feed supplement may provide an interesting source of organic se, and benefit broiler antioxidant system and other biological processes. the objective of this study was to examine the impacts of inorganic se ... | 2017 | 28821244 |
| β-lacta(®) test performance for detection of extended-spectrum-β-lactamase-producing gram negative bacilli directly on bronchial aspirates samples: a validation study. | incidence of extended spectrum β-lactamase-producing gram negative bacilli (esbl-p-gnb)-related infections is worryingly increasing worldwide. esbl-p-gnb detection directly on bronchial aspirate samples (bas) performed for suspected pneumonia may help save empirical carbapenems. our objectives were to optimize β-lacta(®) test (blt) realization and evaluate blt performance for esbl-p-gnb detection directly on bas. | 2017 | 28782646 |
| in vitro and in vivo control of secondary bacterial infection caused by leishmania major. | bacterial infections of cutaneous leishmaniasis cause skin ulcers on mice, resulting in increased tissue deterioration, and these infections can be controlled with liquid allicin. to isolate and identify the incidences of real secondary bacterial infections in mice, we performed the current study by injecting mice (n = 50) with leishmania major. l. major infections were initiated by an intramuscular injection of 0.1 ml roswell park memorial institute (rpmi 1640 media/mouse (10(7) promastigote/ml ... | 2017 | 28703780 |
| tracking the spread routes of opportunistic premise plumbing pathogens in a haematology unit with water points-of-use protected by antimicrobial filters. | water networks in hospitals are frequently contaminated by opportunistic premise plumbing pathogens (oppps) leading to fitment of antimicrobial filters on water points-of-use (pou) in order to limit patients' exposure. | 2017 | 28760634 |
| d-bmap18 antimicrobial peptide is active in vitro, resists to pulmonary proteases but loses its activity in a murine model of pseudomonas aeruginosa lung infection. | the spread of antibiotic resistant-pathogens is driving the search for new antimicrobial compounds. pulmonary infections experienced by cystic fibrosis (cf) patients are a dramatic example of this health-care emergency. antimicrobial peptides could answer the need for new antibiotics but translating them from basic research to the clinic is a challenge. we have previously evaluated the potential of the small membranolytic peptide bmap-18 to treat cf-related infections, discovering that while thi ... | 2017 | 28674688 |
| co-infection of pseudomonas aeruginosa and stenotrophomonas maltophilia in hospitalised pneumonia patients has a synergic and significant impact on clinical outcomes. | ventilator-acquired pneumonia and hospital community-acquired pneumonia are frequently caused by gram-negative and -positive bacteria. we noted that pneumonia patients with co-infection of pseudomonas aeruginosa and stenotrophomonas maltophilia had a poor clinical outcome. to verify this, we retrospectively reviewed pneumonia cases at hebei general hospital from 2010 to 2015. these cases were grouped into four categories: (1) co-infection with p. aeruginosa and s. maltophilia, (2) infection with ... | 2017 | 28669016 |
| activity of innate antimicrobial peptides and ivacaftor against clinical cystic fibrosis respiratory pathogens. | there is a clear need for new antimicrobials to improve current treatment of chronic lung infection in people with cystic fibrosis (cf). this study determined the activities of antimicrobial peptides (amps) and ivacaftor, a novel cf transmembrane conductance regulator potentiator, for cf treatment. antimicrobial activities of amps [ll37, human β-defensins (hβd) 1-4 and slpi] and ivacaftor against clinical respiratory isolates (pseudomonas aeruginosa, staphylococcus aureus, streptococcus spp., ac ... | 2017 | 28666755 |
| meropenem-vaborbactam tested against contemporary gram-negative isolates collected worldwide during 2014, including carbapenem-resistant, kpc-producing, multidrug-resistant, and extensively drug-resistant enterobacteriaceae. | we evaluated the activity of meropenem-vaborbactam against contemporary nonfastidious gram-negative clinical isolates, including enterobacteriaceae isolates with resistance phenotypes and carbapenemase genotypes. meropenem-vaborbactam (inhibitor at 8 μg/ml) and comparators were susceptibility tested by reference broth microdilution methods against 14,304 gram-negative clinical isolates collected worldwide during 2014. carbapenemase-encoding genes were screened by pcr and sequencing. meropenem-va ... | 2017 | 28652234 |
| antimicrobial efficacy of contact lens care solutions against neutrophil-enhanced bacterial biofilms. | neutrophil-derived extracellular debris has been shown to accelerate bacterial biofilm formation on hydrogel and silicone hydrogel contact lens surfaces compared to lenses inoculated with bacteria alone. the purpose of this study was to evaluate the disinfection efficacy of four standard commercial contact lens cleaning regimens against neutrophil-enhanced bacterial biofilms formed on silicone hydrogel contact lenses. | 2017 | 28473944 |
| antibiotic strategies for eradicating pseudomonas aeruginosa in people with cystic fibrosis. | respiratory tract infection with pseudomonas aeruginosa occurs in most people with cystic fibrosis. once chronic infection is established, pseudomonas aeruginosa is virtually impossible to eradicate and is associated with increased mortality and morbidity. early infection may be easier to eradicate.this is an update of a cochrane review first published in 2003, and previously updated in 2006, 2009 and 2014. | 2017 | 28440853 |
| impacts of penicillin binding protein 2 inactivation on β-lactamase expression and muropeptide profile in stenotrophomonas maltophilia. | penicillin binding proteins (pbps) are involved in peptidoglycan synthesis, and their inactivation is linked to β-lactamase expression in ampr-β-lactamase module-harboring gram-negative bacteria. there are seven annotated pbp genes, namely, mrca, mrcb, pbpc, mrda, ftsi, dacb, and dacc, in the stenotrophomonas maltophilia genome, and these genes encode pbp1a, pbp1b, pbp1c, pbp2, pbp3, pbp4, and pbp6, respectively. in addition, s. maltophilia harbors two β-lactamase genes, l1 and l2, whose express ... | 2017 | 28861525 |
| evolutionary genetic analysis uncovers multiple species with distinct habitat preferences and antibiotic resistance phenotypes in the stenotrophomonas maltophilia complex. | the genus stenotrophomonas (gammaproteobacteria) has a broad environmental distribution. stenotrophomonas maltophilia is its best known species because it is a globally emerging, multidrug-resistant (mdr), opportunistic pathogen. members of this species are known to display high genetic, ecological and phenotypic diversity, forming the so-called s. maltophilia complex (smc). heterogeneous resistance and virulence phenotypes have been reported for environmental smc isolates of diverse ecological ... | 2017 | 28861062 |
| synthesis of chitin oligosaccharides using dried stenotrophomonas maltophilia cells containing a transglycosylation reaction-catalyzing β-n-acetylhexosaminidase as a whole-cell catalyst. | bacterial strain nyt501, which we previously isolated from soil, was identified as stenotrophomonas maltophilia, and it was confirmed that this strain produces an intracellular β-n-acetylhexosaminidase exhibiting transglycosylation activity. several properties of this enzyme were characterized using a partially purified enzyme preparation. using n,n'-diacetylchitobiose (glcnac)2 and n,n',n″-triacetylchitotriose (glcnac)3 as substrates and dried cells of this bacterium as a whole-cell catalyst, c ... | 2017 | 28840460 |
| background nutrients affect the biotransformation of tetracycline by stenotrophomonas maltophilia as revealed by genomics and proteomics. | certain bacteria are resistant to antibiotics and can even transform antibiotics in the environment. it is unclear how the molecular mechanisms underlying the resistance and biotransformation processes vary under different environmental conditions. the objective of this study is to investigate the molecular mechanisms of tetracycline resistance and biotransformation by stenotrophomonas maltophilia strain dt1 under various background nutrient conditions. strain dt1 was exposed to tetracycline for ... | 2017 | 28837770 |
| stenotrophomonas bentonitica sp. nov., isolated from bentonite formations. | a gram-stain negative, rod-shaped, aerobic bacterial strain, bii-r7t, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern spain. comparative 16s rrna gene sequence analysis showed that bii-r7t represented a member of the genus stenotrophomonas (class gammaproteobacteria), and was related most closely to stenotrophomonas rhizophila e-p10t (99.2 % sequence similarity), followed by stenotrophomonas pavanii icb 89t (98.5 %), ... | 2017 | 28820086 |
| incident stenotrophomonas maltophilia infection and lung function decline in cystic fibrosis. | to determine whether incident detection of stenotrophomonas maltophilia (sm) in patients with cystic fibrosis (cf) is associated with accelerated lung function decline and increased hospitalizations and to determine whether this effect is more pronounced in individuals with subsequent chronic infection. | 2017 | 28815999 |
| avibactam restores the susceptibility of aztreonam against clinical isolates of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an emerging opportunistic pathogen, classified by the world health organization as one of the leading multidrug resistant organisms in hospital settings. discovery of novel compounds and/or combination therapies for s. maltophilia is urgent. we demonstrate the in-vitro efficacy of aztreonam-avibactam (atm-avi) against s. maltophilia, and kinetically characterize the inhibition of the l2 β-lactamase by avibactam. atm-avi overcomes aztreonam resistance in selected c ... | 2017 | 28784669 |
| risk factors and molecular mechanisms associated with trimethoprim-sulfamethoxazole resistance in stenotrophomonas maltophilia in mexico. | stenotrophomonas maltophilia is a multidrug-resistant opportunistic pathogen causing an increasing number of nosocomial infections. our aim was to evaluate the risk factors and mechanisms associated with trimethoprim-sulfamethoxazole (sxt) resistance in s. maltophilia infections in mexico. | 2017 | 28771141 |
| stenotrophomonas maltophilia is highly prevalent among houseflies (musca domestica). | stenotrophomonas maltophilia is capable of surviving in a wide variety of environments and is considered to be among the antimicrobial-resistant bacteria of greatest public health concern in hospital settings. to clarify the role of houseflies (musca domestica) in disseminating this bacterium, we collected 99 individuals from 15 locations (9 farms and 6 urban areas) in thailand. s. maltophilia was isolated from 39 % (39/99) of these houseflies, with the isolation rates being similar in farms and ... | 2017 | 28771133 |
| distribution of class i integron and smqnr resistance gene among stenotrophomonas maltophilia isolated from clinical samples in iran. | stenotrophomonas maltophilia (s. maltophilia) is a multiple-antibiotic-resistant opportunistic pathogen that is being isolated with increasing frequency from patients with health-care-associated infections. s. maltophilia is inherently resistant to most of the available antimicrobial agents. spread of resistant strains has been attributed, in part, to class i integrons. in vitro susceptibility studies have shown trimethoprim-sulfamethoxazole and new floroquinolones as two important agents with a ... | 2017 | 28706609 |
| risk factors for hospital acquisition of trimethoprim-sulfamethoxazole resistant stenotrophomonas maltophilia in adults: a matched case-control study. | the emergence of trimethoprim-sulfamethoxazole resistant stenotrophomonas maltophilia (tsrsm) represents a serious threat to patients. the aim of current study was to identify risk factors associated with hospital-acquired tsrsm occurrence in adult inpatients. | 2017 | 28688829 |
| bsmr degrades c-di-gmp to modulate biofilm formation of nosocomial pathogen stenotrophomonas maltophilia. | c-di-gmp is a cellular second messenger that regulates diverse bacterial processes, including swimming, biofilm formation and virulence. however, in stenotrophomonas maltophilia, a nosocomial pathogen that frequently infects immunodeficient or immunoincompetent patients, the regulatory function of c-di-gmp remains unclear. here we show that bsmr is a negative regulator of biofilm development that degrades c-di-gmp through its eal domain. increasing bsmr expression resulted in significant increas ... | 2017 | 28680041 |
| [lawn mower injury in children - a lesson for life]. | | 2017 | 28656410 |
| distribution and characterization of stenotrophomonas maltophilia isolates from environmental and clinical samples in thailand. | stenotrophomonas maltophilia has emerged as an important opportunistic pathogen, especially in patients who are immunocompromised, suffering from malignancy or have been hospitalized for a prolonged period. information of this bacterium in thailand has not been elucidated. | 2017 | 28602707 |
| stenotrophomonas maltophilia: a rare cause of peritonitis in capd patients. | | 2017 | 28578862 |
| molecular epidemiology and characteristics of 16 cases with stenotrophomonas maltophilia bacteraemia in pediatric intensive care units. | recently, stenotrophomonas maltophilia has increasingly been reported as an important nosocomial opportunistic pathogen. limited therapeutic options of s. maltophilia infections demand early identification and knowledge about the probable risk factors for controlling its spread. | 2017 | 28569336 |
| biodegradation of α-endosulfan via hydrolysis pathway by stenotrophomonas maltophilia og2. | stenotrophomonas maltophilia og2 was isolated from the intestine of cockroaches that was collected from a cow barn contaminated some pesticides belong to pyrethroid and organochlorine groups. og2 was able to degrade α-endosulfan in non sulfur medium (nsm) as a sole sulfur source for growth within 10 days of incubation. the effects of some growth parameters on endosulfan biodegradation by og2 was studied and found that the biodegradation was significantly affected by the endosulfan concentrations ... | 2017 | 28567625 |
| unusual case of rapid growing intraabdominal abscess caused by stenotrophomonas maltophilia after laparoscopic appendectomy due to perforated appendicitis: a case report. | an intraabdominal abscess due to stenotrophomonas maltophilia (s maltophilia) infection is a very rare clinical manifestation. s maltophilia is a glucose nonfermentative, aerobic, gram-negative, mobile, and biofilm-forming bacterium. it is an opportunistic pathogen and uncommon cause of infection. respiratory tract infections (pneumonia) and bloodstream infections (bacteremia) are the most common clinical manifestations of s maltophilia infection. | 2017 | 28514306 |
| stenotrophomonas maltophilia in a university hospital of traditional chinese medicine: molecular epidemiology and antimicrobial resistance. | stenotrophomona maltophilia has emerged as an important opportunistic pathogen that is highly antibiotic resistant. analysis of antibiotic susceptibilities, drug-resistant gene profiles and molecular typing of s. maltophilia was undertaken in a university hospital of traditional chinese medicine in east china. resistance to sulphamethoxazole (sxt) was found to be an indicator of multi-drug resistance. sxt resistance was mediated by sul and dfra genes in integrons, especially class 1. some eviden ... | 2017 | 28502480 |
| membrane vesicle secretion and prophage induction in multidrug-resistant stenotrophomonas maltophilia in response to ciprofloxacin stress. | several bacterial species produce membrane vesicles (mvs) in response to antibiotic stress. however, the biogenesis and role of mvs in bacterial antibiotic resistance mechanisms have remained unclear. here, we studied the effect of the fluoroquinolone ciprofloxacin on mv secretion by stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. we found that in addition to the classical outer membrane vesicles (omv), ciprofloxacin-stimulated cultures produced ... | 2017 | 28488744 |
| rapidly progressive hemorrhagic pneumonia caused by stenotrophomonas maltophilia occurring early after bone marrow transplantation in a patient with myelodysplastic syndrome. | a 44-year-old woman had repeated bacterial infections because of hypoplastic myelodysplastic syndrome; therefore, she underwent allogeneic hematopoietic cell transplantation. broad spectrum antibiotics were administered because she had bacterial infection and pneumonia 2 weeks before undergoing transplantation. on day19 after transplantation, she suddenly presented with hemoptysis. stenotrophomonas maltophilia was considered to be the cause of hemorrhagic pneumonia, which required ventilation su ... | 2017 | 28484160 |
| retraction for mccarthy et al., "the ax21 protein is a cell-cell signal that regulates virulence in the nosocomial pathogen stenotrophomonas maltophilia". | | 2017 | 28442539 |
| emergence of stenotrophomonas maltophilia nosocomial isolates in a saudi children's hospital. risk factors and clinical characteristics. | to describe the clinical characteristics of pediatric patients colonized or infected by stenotrophomonas maltophilia (s. maltophilia) at a saudi children's hospital, to identify risk factors associated with infection, and to investigate the antimicrobial resistance patterns of this emerging pathogen. methods: in this cross-sectional observational study, 64 non-duplicating s. maltophilia strains were isolated in najran maternity and children's hospital, najran, saudi arabia between january 201 ... | 2017 | 28439603 |
| a chromatographic approach to distinguish gram-positive from gram-negative bacteria using exogenous volatile organic compound metabolites. | this paper utilized l-alanine aminopeptidase activity as a useful approach to distinguish between gram-negative and gram-positive bacteria. this was done using two enzyme substrates, specifically 2-amino-n-phenylpropanamide and 2-amino-n-(4-methylphenyl)propanamide which liberated the volatile compounds aniline and p-toluidine, respectively. two complementary analytical techniques have been used to identify and quantify the vocs, specifically static headspace multicapillary column gas chromatogr ... | 2017 | 28438317 |