Publications

TitleAbstractYear
Filter
PMID
Filter
development of broad-range polymerase chain reaction (pcr) bacterial identification in diagnosis of infective endocarditis.the study aim was to identify the value of broad-range bacterial pcr in infective endocarditis (ie) of bacterial etiology, and to determine its specificity and sensitivity.200212479291
synergy of colistin with rifampin and trimethoprim/sulfamethoxazole on multidrug-resistant stenotrophomonas maltophilia.stenotrophomonas maltophilia is characterized by intrinsic resistance to a variety of antimicrobials. therapeutic options are often limited particularly after the emergence of isolates resistant to trimethoprim/sulfamethoxazole. the application of colistin for infections caused by multidrug-resistant gram-negative pathogens is limited due to its toxicity. in order to evaluate the activity of the interaction of colistin with rifampin or trimethoprim/sulfamethoxazole on s. maltophilia, 24 differen ...200212493173
activities of the glycylcycline tigecycline (gar-936) against 1,924 recent european clinical bacterial isolates.the in vitro activities of tigecycline against 1,924 clinical isolates were examined. the new glycylcycline exhibited excellent activity against all gram-positive cocci (mics at which 90% of the isolates tested were inhibited [mic(90)s], <or=1 microg/ml). in addition, it was also very potent against most members of the enterobacteriaceae, with most mic(90)s being <or=2 microg/ml. among the nonfermenters, acinetobacter spp. and stenotrophomonas maltophilia are included in the in vitro spectrum of ...200312499224
a comparison of pulmonary exacerbations with single and multiple organisms in patients with cystic fibrosis and chronic burkholderia cepacia infection.to compare pulmonary exacerbations with single and multiple organisms in patients with chronic burkholderia cepacia infection.200312504610
three-dimensional structure of fez-1, a monomeric subclass b3 metallo-beta-lactamase from fluoribacter gormanii, in native form and in complex with d-captopril.the beta-lactamases are involved in bacterial resistance to penicillin and related compounds. members of the metallo-enzyme class are now found in many pathogenic bacteria and are thus becoming of major clinical importance. the structures of the zn-beta-lactamase from fluoribacter gormanii (fez-1) in the native and in the complex form are reported here. fez-1 is a monomeric enzyme, which possesses two zinc-binding sites. these structures are discussed in comparison with those of the tetrameric l ...200312507470
[microbiology and cystic fibrosis].the respiratory infections are the rule in sick persons of cystic fibrosis. after the colonization in one's early childhood by staphylococcus aureus and haemophilus influenzae, it is established the colonization produced by the mucous strains of pseudomonas aeruginosa, the principal etiogenic of this illness, that can become to isolate in more than 95% of sick persons at three years old. that's why the etiogenic factors of this bacterium are studied, as they are toxins, pigments, enzymes, and pr ...200212518659
mutations in gyra and parc qrdrs are not relevant for quinolone resistance in epidemiological unrelated stenotrophomonas maltophilia clinical isolates.clinical strains of stenotrophomonas maltophilia are often highly resistant to multiple antibiotics and this resistance is steadily rising. quinolones are included in the group of antimicrobial agents to which this microorganism is developing resistance. therefore, the aim of this study was to analyze the epidemiological relationship among 22 clinical isolates of s. maltophilia as well as the molecular mechanisms responsible for the acquisition of quinolone-resistance in these strains. the resul ...200212523620
enhanced membrane permeabilization and antibacterial activity of a disulfide-dimerized magainin analogue.a cysteine substitution analogue of magainin-2 amide (magainin-f12w, n22c; denoted here as mag-n22c), and a disulfide-linked dimer prepared by air oxidation [(mag-n22c)(2)], were compared in their ability to release carboxyfluorescein (cf) from 100-nm large unilamellar vesicles (luv) and to kill the gram negative bacteria stenotrophomonas maltophilia and escherichia coli. the disulfide-dimerized peptide showed enhanced permeabilization and antimicrobial activity, when compared with the monomeric ...200312525167
antimicrobial susceptibility profile of molecular typed cystic fibrosis stenotrophomonas maltophilia isolates and differences with noncystic fibrosis isolates.multiresistance in stenotrophomonas maltophilia limits the effectiveness of antimicrobial therapy for infections due to this organism. it can be of special concern in cystic fibrosis (cf) patients due to frequent antimicrobial administration. the in vitro activity of 41 antimicrobial agents against 76 epidemiologically defined cf s. maltophilia isolates by pulsed-field-gel electrophoresis (pfge) technique under xbai and spei restriction was compared with that obtained with 51 non-cf strains reco ...200312526070
successful treatment of multi-resistant stenotrophomonas maltophilia meningitis with ciprofloxacin in a pre-term infant. 200212536991
levofloxacin and ciprofloxacin in vitro activities against 4,003 clinical bacterial isolates collected in 24 italian laboratories.levofloxacin showed comparable in vitro susceptibility to ciprofloxacin among enterobacteriaceae, pseudomonas aeruginosa, enterococci, and staphylococcus aureus, while greater susceptibility was observed in stenotrophomonas maltophilia and staphylococcus epidermidis, mainly when oxacillin resistant. the susceptibility of streptococcus pneumoniae to levofloxacin reached 99%.200312543701
[transfer and expression of tyrosinase gene of pseudomonas maltophilia in pseudomonas pseudoalcaligenes].pseudomonas pseudoalcaligenes is a notably killing maggots bacterium, which was isolated from natural dead maggots in the manure pits in the countryside of yancheng. it easily die from effect of ultra-violet ray when it is exposed in the sun. antiradiation effect of melanin is quite strong. mel gene of p. maltophilia at18 has been introduced into p. pseudoalcaligenes, and enabled it the ability of producing melanin steadily. southern hybridization studies confirmed that the small fragment cloned ...200112552802
nosocomial stenotrophomonas maltophilia strain with a broad host range ability to transfer antibiotic resistance. 200212583560
involvement of a quinoprotein (pqq-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium stenotrophomonas maltophilia.previous work has shown that when the bacterium stenotrophomonas maltophilia is grown on polypropylene glycol, different dye-linked polypropylene glycol dehydrogenase (ppg-dh) activities are induced during growth. here the purification and characterization of the dehydrogenase activity induced in the stationary phase, and present in the periplasmic space, is described. the homogeneous enzyme preparation obtained consists of a homodimeric protein with a molecular mass of about 123 kda and an isoe ...200312586415
stenotrophomonas maltophilia endocarditis.stenotrophomonas maltophilia is a gram-negative bacillus that is increasingly associated with serious nosocomial infections, especially in immunocompromised patients; however, the occurrence of endocarditis due to this organism is rare. this report describes a case of s. maltophilia endocarditis associated with a central venous catheter. the literature on stenotrophomonas endocarditis is reviewed. given the high morbidity and mortality of these infections, early antibiotic therapy utilizing trim ...200212587628
the influence of the chemical composition of drinking water on cuprosolvency by biofilm bacteria.this study investigated the influence of water chemistry on copper solvation (cuprosolvency) by pure culture biofilms of heterotrophic bacteria isolated from copper plumbing.200312588559
bacteraemia in adults due to glucose non-fermentative gram-negative bacilli other than p. aeruginosa.glucose non-fermentative gram-negative bacilli other than p. aeruginosa (nf) are emerging pathogens.200312615987
safety and use of sputum induction in children with cystic fibrosis.we assessed the safety and use of induced sputum (is) in children with cystic fibrosis (cf). forty-eight children (19 males) with cf, mean age 12.6 (range, 7.3-17.0) years and median forced expired volume in 1 sec (fev(1)) 48% (range, 14-77%) predicted were recruited. patients spontaneously expectorated sputum and then performed sputum induction by inhalation of nebulized 7% hypertonic saline. samples were sent for bacteriological culture, and for measurement of the following inflammatory mediat ...200312629630
fatty acid analysis of stenotrophomonas maltophilia clinical strains showing different susceptibility to antibiotics at 30 and 37 degrees c.isolates of stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (tds) to antibiotics. both 30tds strains (at least 4 times lower value of minimum inhibitory concentration (mic) of an antibiotic at 30 than at 37 degrees c) and 37tds strains (at least 4 times lower value of mic at 37 than at 30 degrees c) were described. changes in the distribution of saturated and unsaturated fatty acids (fa) at 30 and 37 degrees c were considered as one of possible cau ...200212630330
oral colonisation by aerobic and facultatively anaerobic gram-negative rods and yeast in tibetans living in lhasa.sample groups of children (n=50) and adults (n=38) were selected from pools of 207 children, (11-13-year olds from two primary schools) and 94 adults (25-44-year olds from four governmental agencies) who were the subjects of an oral health survey among tibetans living in lhasa, tibet autonomous region. mean ages of the study groups of children (38% females) and adults (61% females) were 11.6+/-0.9 and 37.1+/-6.1 years, respectively. all had lived in tibet since birth. oral rinse samples were sel ...200312642230
the efflux pump inhibitor phe-arg-beta-naphthylamide does not abolish the activity of the stenotrophomonas maltophilia smedef multidrug efflux pump. 200312654744
role of the acetyltransferase aac(6')-iz modifying enzyme in aminoglycoside resistance in stenotrophomonas maltophilia.stenotrophomonas maltophilia is an emerging nosocomial pathogen that displays high-level intrinsic resistance to multiple antibiotics including aminoglycosides. a gene [aac(6')-iz] encoding an aminoglycoside-modifying enzyme, aac(6')-iz acetyltransferase, was recently cloned and sequenced in s. maltophilia, but its importance with respect to aminoglycoside resistance in this organism was not determined. using a homologous gene replacement approach, mutants carrying unmarked chromosomal deletions ...200312654758
liver abscess caused by stenotrophomonas maltophilia: report of a case.we report the case of a melioidosis-like abscess of the liver caused by stenotrophomonas (xanthomonas) maltophilia infection in a chinese man living in hungary. although this appears to be the first documentation of a liver abscess of this origin in a nonimmunocompromised patient, our case report demonstrates that this common facultative pathogen can also cause liver abscess and sepsis. after repeated negative blood cultures, histological examinations of liver biopsies suggested the possibility ...200312658392
negative bacterial polymerase chain reaction (pcr) findings in prostate tissue from patients with symptoms of chronic pelvic pain syndrome (cpps) and localized prostate cancer.the etiology of chronic pelvic pain syndrome (cpps) remains obscure. although, bacterial etiology has frequently been suggested, evidence of both bacterial involvement in cpps and the presence of normal bacterial flora in the prostate remain uncertain.200312661035
transformations of selenate and selenite by stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment.a gram-negative bacterium, identified as stenotrophomonas maltophilia by fatty acid analysis and 16s rrna sequencing, was isolated from a seleniferous agricultural evaporation pond sediment collected in the tulare lake drainage district, california. in cultures exposed to the atmosphere, the organism reduces selenate (seo4(2-)) and selenite (seo3(2-)) to red amorphous elemental selenium (se degrees ) only upon reaching stationary phase, when o2 levels are less than 0.1 mg l(-1). in 48 h, s. malt ...200312662176
an outbreak of lymphadenitis associated with stenotrophomonas (xanthomonas) maltophilia in omani goats.stenotrophomonas maltophilia is a commonly isolated organism from human clinical specimens and is gaining significance as a pathogen in immunocompromised patients and nosocomial infections. in most cases it is difficult to establish the source of human infections. in veterinary medicine, s. maltophilia is not generally considered a primary pathogen. in the present study, we report the occurrence of 16 cases of caprine abscess from which s. maltophilia was isolated in pure culture from 15 animals ...200312675904
microbial contamination of embryos and semen during long term banking in liquid nitrogen.we report on microbial contamination of embryos and semen cryopreserved in sealed plastic straws and stored for 6-35 years in liquid nitrogen. there were 32 bacterial and 1 fungal species identified from randomly drawn liquid nitrogen, frozen semen, and embryos samples stored in 8 commercial and 8 research facility liquid nitrogen (ln) tanks. the identified bacteria represented commensal or environmental microorganisms and some, such as escherichia coli, were potential or opportunistic pathogens ...200312686204
[indications and results of retroperitoneal laparostomy in the treatment of infected acute necrotizing pancreatitis].the aim of this study is to describe personal experience with retroperitoneal laparostomy in the management of infected acute necrotizing pancreatitis. the presence of an infected phlegmon requires surgical debridement and drainage. the surgical approach can be either an anterior laparotomy with irrigation and drainage (which can be either an open or closed laparotomy) or a posterior laparostomy. three patients (2 men and 1 woman) presented with an unfavourable course of their acute necrotizing ...200312692503
in vitro activity of gatifloxacin alone and in combination with cefepime, meropenem, piperacillin and gentamicin against multidrug-resistant organisms.to study the in vitro interaction of gatifloxacin in combination with gentamicin and with the beta-lactams cefepime, meropenem and piperacillin against clinical isolates of stenotrophomonas maltophilia, pseudomonas aeruginosa, burkholderia cepacia, extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae, vancomycin-resistant enterococcus faecium (vre) and methicillin-resistant staphylococcus aureus (mrsa).200312697632
risk factors for stenotrophomonas maltophilia bacteremia in oncology patients: a case-control study.to characterize risk factors for stenotrophomonas maltophilia bloodstream infection in oncology patients.200312725356
comparative in vitro potency of amoxycillin-clavulanic acid and four oral agents against recent north american clinical isolates from a global surveillance study.the in vitro activity of amoxycillin-clavulanic acid was compared with four comparator oral antimicrobial agents; ampicillin, azithromycin, cefuroxime and trimethoprim-sulphamethoxazole against 4536 recent clinical isolates covering 29 species isolated in the us and canada between 1997 and 1999. based upon minimum inhibitory concentrations (mics), amoxycillin-clavulanic acid was the most active agent against many gram-positive species and phenotypes including methicillin susceptible staphylococc ...200312727075
effects of permeabilizers on antimicrobial susceptibility of stenotrophomonas maltophilia and acinetobacter spp.isolates of the gram-negative bacteria stenotrophomonas maltophilia and acinetobacter spp. are naturally resistant to many classes of antibiotics. using the disk diffusion technique it was shown that the membrane permeabilizers ethylenediaminetetraacetic acid, sodium citrate, and sodium polyphosphate increased susceptibility to a range of antibiotics including imipenem, ciprofloxacin, tetracycline, and rifampicin. these effects are probably due to the metal chelating properties of the permeabili ...200312741739
stenotrophomonas maltophilia septicemia with pyomyositis in a chemotherapy-treated patient.pyomyositis is a rare complication of chemotherapy. a 35-year-old male patient with myelodysplastic syndrome developed stenotrophomonas maltophilia bacteremia shortly after chemotherapy, and stenotrophomonas maltophilia-related pyomyositis was encountered after recovery from neutropenia. he recovered completely after surgical drainage and a protracted course of antibiotic treatment. it is postulated that subclinical myopathy, immunosuppression secondary to the malignancy, or chemotherapeutic dru ...200312750843
taxonomic positioning of two biological control agents for plant diseases as lysobacter enzymogenes based on phylogenetic analysis of 16s rdna, fatty acid composition and phenotypic characteristics.to taxonomically position two bacterial strains conferring biological control activity towards plant diseases.200312752818
role of phosphoglucomutase of stenotrophomonas maltophilia in lipopolysaccharide biosynthesis, virulence, and antibiotic resistance.a homologue of the algc gene, responsible for the production of a phosphoglucomutase (pgm) associated with lps and alginate biosynthesis in pseudomonas aeruginosa, spgm, was cloned from stenotrophomonas maltophilia. the spgm gene was shown to encode a bifunctional enzyme with both pgm and phosphomannomutase activities. mutants lacking spgm produced less lps than the spgm(+) parent strain and had a tendency for shorter o polysaccharide chains. no changes in lps chemistry were obvious as a result ...200312761084
infection control recommendations for patients with cystic fibrosis: microbiology, important pathogens, and infection control practices to prevent patient-to-patient transmission. 200312762292
stenotrophomonas maltophilia infection related mortality during induction in childhood acute lymphoblastic leukemia. 200312764762
synthetic peptide vaccine and antibody therapeutic development: prevention and treatment of pseudomonas aeruginosa.pseudomonas aeruginosa and pseudomonas maltophilia account for 80% of opportunistic infections by pseudomonads. pseudomonas aeruginosa is an opportunistic pathogen that causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, and a variety of systemic infections, particularly in patients with severe burns, and in cancer and aids patients who are immunosuppressed. pseudomonas aeruginosa is notable for its resistance to antibiotics, and is the ...200312767116
stenotrophomonas (xanthomonas) maltophilia as an emerging opportunistic pathogen in association with hiv infection: a 10-year surveillance study.stenotrophomonas (xanthomonas) maltophilia has been increasingly reported as a nosocomial opportunistic pathogen, responsible for serious infectious complications in immunocompromised patients. at present very limited information is available concerning its clinical significance in the setting of hiv infection.200312789473
microbial consortia that degrade 2,4-dnt by interspecies metabolism: isolation and characterisation.two consortia, isolated by selective enrichment from a soil sample of a nitroaromatic-contaminated site, degraded 2,4-dnt as their sole nitrogen source without accumulating one or more detectable intermediates. though originating from the same sample, the optimised consortia had no common members, indicating that selective enrichment resulted in different end points. consortium 1 and consortium 2 contained four and six bacterial species respectively, but both had two members that were able to co ...200312801097
development of a gram-negative selective agar (gnsa) for the detection of gram-negative microflora in sputa in patients with cystic fibrosis.to develop a selective agar medium to help detect and quantify gram-negative flora in the sputum of patients with cystic fibrosis (cf).200312807467
occurrence and antimicrobial resistance of gram-negative bacteria isolated in haemodialysis water and dialysate of renal units: results of a greek multicentre study.to evaluate the occurrence, identity and antimicrobial resistance of gram-negative bacteria isolated from municipal water supplies, treated water, and dialysate of all 85 greek haemodialysis centres.200312807469
molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses.chronic maxillary sinusitis is a chronic inflammatory condition in which the role of microbial infection remains undefined. bacteria have been isolated from chronically inflamed sinuses; however, their role in the chronicity of inflammation is unknown. the objective of this study was to determine whether bacteria are present in clinical samples from chronic maxillary sinusitis and to assess the diversity of the flora present. washes and/or tissue samples from endoscopic sinus surgery on 11 patie ...200312808082
[continuous surveillance of antimicrobial resistance among nosocomial gram-negative bacilli from intensive care units in china].to investigate the change of antimicrobial resistance among nosocomial gram-negative bacilli, especially those of enterobacteriaceae isolated from intensive care units from 1994 to 2001 in china.200312820912
[changes of antimicrobial resistance among nonfermenting gram-negative bacilli isolated from intensive care units from 1994 to 2001 in china].to investigate the antimicrobial resistance among nonfermenting gram-negative bacilli isolated from intensive care units (icus) during the past 7 years in china.200312820914
differences in the permeability of high-flux dialyzer membranes for bacterial pyrogens.the increasing use of high-flux membranes for hemodialysis has raised concerns that patients dialyzed with these membranes may be at higher risk of being exposed to cytokine-inducing bacterial substances in the dialysate than patients dialyzed with low-flux membranes. we investigated the permeability of various high-flux membranes for both purified e. coli lipopolysaccharide (lps) as well as for lps derived from stenotrophomonas (sten.) maltophilia.200312834177
clinical role of beta-lactam/beta-lactamase inhibitor combinations.the use of beta-lactamase inhibitors in combination with beta-lactam antibiotics is currently the most successful strategy to combat a specific resistance mechanism. their broad spectrum of activity originates from the ability of respective inhibitors to inactivate a wide range of beta-lactamases produced by gram-positive, gram-negative, anaerobic and even acid-fast pathogens. clinical experience confirms their effectiveness in the empirical treatment of respiratory, intra-abdominal, and skin an ...200312834367
[endocarditis caused by stenotrophomonas maltophilia: report of a case and review of literature].we report a case of a 65 year-old woman with late endocarditis of prosthetic aortic valve and paravalvular abscess by stenotrophomonas maltophilia, which had an acute presentation for the memtionated abscess broken, with fever, bacteremia and congestive heart failure secondary to severity aortic regurgitation. itacute;s a rare cause of endocarditis with only 22 cases descripted in medical literature, the most of them in parenteral drug addict and as complication of cardiac valve replacement. the ...200312848604
the differential beta-lactamase activity of stenotrophomonas maltophilia. 200312855245
antimicrobial activity and interference of tobramycin and chloramphenicol on bacterial adhesion to intraocular lenses.the antimicrobial activities of tobramycin and chloramphenicol were evaluated by determining minimum inhibitory and bactericidal concentrations against staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, group a, group b and group g streptococci, klebsiella spp., stenotrophomonas maltophilia and ciprofloxacin-resistant and -susceptible pseudomonas aeruginosa, as well as by evaluating interference on adhesion of slime producer strains of s. aureus and p. aeruginosa to intrao ...200312866361
evaluation of the bd phoenix automated identification and susceptibility testing system in clinical microbiology laboratory practice.the phoenix automated microbiology system (bd biosciences, usa) is a new, fully automated system for the rapid identification and antimicrobial susceptibility testing of gram-positive and gram-negative bacteria. the objective of this study was to evaluate the quality of performance of the phoenix system in the identification and antimicrobial susceptibility testing of 260 gram-negative ( n=174) and gram-positive ( n=86) isolates collected from polish hospitals in recent years. two phoenix panel ...200312884060
novel nosocomial infections by stenotrophomonas maltophilia: first reported case from lucknow, north india.we describe a case of empyema with infected ascites caused by stenotrophomonas maltophilia, which has rarely been reported as pathogenic. the source was determined to a disinfectant solution. the isolate was sensitive to a newer carbapenem-meropenem, and the patient was treated successfully. this case represents a novel dual presentation of a nosocomial infection by the isolate in question.200312904437
efflux as a mechanism of resistance to antimicrobials in pseudomonas aeruginosa and related bacteria: unanswered questions.pseudomonas aeruginosa is an opportunistic human pathogen exhibiting innate resistance to multiple antimicrobial agents. this intrinsic multidrug resistance is caused by synergy between a low-permeability outer membrane and expression of a number of broadly-specific multidrug efflux (mex) systems, including mexab-oprm and mexxy-oprm. in addition to this intrinsic resistance, these and three additional systems, mexcd-oprj, mexef-oprn and mexjk-oprm promote acquired multidrug resistance as a conse ...200312917802
systemic diseases in association with microbial species in oral biofilm from elderly requiring care.the oral cavity is a reservoir for colonization and infection of systemic organs by pathogenic bacteria. it is understood that aging, tooth eruption, hormonal changes, active disease, oral hygiene, and other factors have an influence on biofilm formation and bacterial accumulation in the oral cavity.200312920350
fimbriae and adherence of stenotrophomonas maltophilia to epithelial cells and to abiotic surfaces.stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen associated with several infectious diseases and opportunistic infections, especially in immunocompromised patients. these bacteria adhere avidly to medical implants and catheters forming a biofilm that confers natural protection against host immune defences and different antimicrobial agents. the nature of the bacterial surface factors involved in biofilm formation on inert surfaces and in adherence of s. maltophilia to ep ...200312925132
[three cases of stenotrophomonas maltophilia pneumonia].we encountered 3 cases of pneumonia caused by stenotrophomonas maltophilia between january and june 2001. s. maltophilia is resistant to broad-spectrum antibiotics including carbapenem. reported studies indicate that excessive use of broad-spectrum antibiotics may induce resistance in this organism. however, our data showed that there was no clear correlation between the amounts of carbapenems used in our hospital and the isolation of the organism. if broad-spectrum antibiotics are ineffective o ...200312931674
[phenotypic and genotypic characteristics of non fermenting atypical strains recovered from cystic fibrosis patients].we used partial 16s rrna gene (16s dna) sequencing for the prospective identification of nonfermenting gram-negative bacilli recovered from patients attending our cystic fibrosis center (hôpital necker-enfants malades), which gave problematic results with conventional phenotypic tests. during 1999, we recovered 1093 isolates of nonfermenting gram-negative bacilli from 702 sputum sampled from 148 patients. forty-six of these isolates (27 patients) were not identified satisfactorily in routine lab ...200312948761
pseudomonas aeruginosa and burkholderia cepacia cannot be detected by pcr in the breath condensate of patients with cystic fibrosis.the collection of sputum for microbiological examination in young cystic fibrosis patients can be very difficult. however, a knowledge of bacterial flora colonizing the patient's airways is of paramount importance for proper antimicrobial therapy. it is also known that cystic fibrosis patients colonized by pseudomonas species have a poorer prognosis than pseudomonas-negative patients. noninvasive ways of diagnosing airway inflammation that require only minimal cooperation of the patient might yi ...200312950050
partial characterization of chorionic gonadotropin-like binding sites from the bacteria xanthomonas maltophilia.the gram-negative bacterium, xanthomonas maltophilia, has low- and high-affinity luteinizing hormone/chorionic gonadotropin (lh/cg)-binding sites, similar to the lh/cg receptor found in mammals. although the low-affinity site binds both lh and human cg (hcg), the high-affinity site is specific for hcg. in the current investigation, these two binding sites were independently isolated from x. maltophilia for further characterization. to isolate functional binding sites, we developed a solubilizati ...200312968064
the in-vitro antimicrobial effect of non-antibiotics and putative inhibitors of efflux pumps on pseudomonas aeruginosa and staphylococcus aureus.the anti-microbial activity of six non-antibiotics (one amino-ethylchloride, three phenothiazines, two tricyclic antidepressives) were tested on 20 clinical isolates of pseudomonas aeruginosa, one clinical isolate of klebsiella pneumoniae, 2 attc strains and 14 clinical isolates of staphylococccus aureus, using the plate dilution method. the effects on p. aeruginosa were independent of antibiotic resistance pattern and the species stenotrophomonas maltophilia was found to be the most susceptible ...200313678831
[on the isolation of pseudomonas maltophilia from clinical examination material]. 196514272024
pseudomonas maltophilia, an alcaligenes-like species. 196114449786
antimicrobial resistance among gram-negative organisms in the intensive care unit.we review the hospital-acquired gram-negative organisms commonly encountered among patients in the intensive care unit and discuss pertinent surveillance data, resistance mechanisms and patterns, and optimal treatment regimens for these pathogens.200314508155
the susceptibility of non-fermentative gram-negative bacilli to cefperazone and sulbactam compared with other antibacterial agents.this study was designed to determine the bacterial susceptibility of non-fermentative gram-negative organisms to various antibacterial agents. bacterial susceptibility testing used the kirby-bauer method and data were assessed according to nccls 2000. cefoperazone/sulbactam (cper/su) had good antibacterial activity against pseudomonas aeruginosa. its activity was next only to that of imipenem, meropenem and ceftazidime. cper/su was highly active against acinetobacter spp., alcaligenes spp., burk ...200314522108
in vitro antimicrobial activity of moxifloxacin compared to other quinolones against recent clinical bacterial isolates from hospitalized and community-based cancer patients.the in vitro spectrum of moxifloxacin (a c-8-methoxyquinolone) was compared to that of ciprofloxacin and levofloxacin against 924 recent clinical isolates from cancer patients. moxifloxacin was more active than the comparator agents against gram-positive pathogens, with potent activity against aerococcus spp., listeria monocytogenes, micrococcus spp., rhodococcus equi, and stomatococcus mucilaginous, methicillin-susceptible staphylococcus spp., all beta hemolytic streptococci, viridans streptoco ...200314522520
stenotrophomonas maltophilia intestinal colonization in hospitalized oncology patients with diarrhea.a 6-week surveillance study was performed to determine the prevalence of stenotrophomonas maltophilia intestinal colonization among patients hospitalized in an oncology unit who developed diarrhea. ninety-two stool samples obtained from 41 patients were cultured, and 4 patients (4 [9.5%] of 41 patients) had cultures that were positive for s. maltophilia. after controlling for duration of diarrhea, patients colonized with s. maltophilia had received a greater number of different types of antibiot ...200314523780
acute tubular necrosis associated with propylene glycol from concomitant administration of intravenous lorazepam and trimethoprim-sulfamethoxazole.a 46-year-old morbidly obese man was admitted to the medical intensive care unit with respiratory failure. he required pressure-control ventilation and high levels of sedation with continuous-infusion lorazepam. he developed stenotrophomonas maltophilia pneumonia; treatment included scheduled intravenous trimethoprim-sulfamethoxazole. each of these drugs contain several hundred milligrams/milliliter of propylene glycol. on day 17 of his hospital course, 3 days after starting the trimethoprim-sul ...200314524651
pathophysiology and management of pulmonary infections in cystic fibrosis.this comprehensive state of the art review summarizes the current published knowledge base regarding the pathophysiology and microbiology of pulmonary disease in cystic fibrosis (cf). the molecular basis of cf lung disease including the impact of defective cystic fibrosis transmembrane regulator (cftr) protein function on airway physiology, mucociliary clearance, and establishment of pseudomonas aeruginosa infection is described. an extensive review of the microbiology of cf lung disease with pa ...200314555458
stenotrophomonas maltophilia: a serious and rare complication in patients suffering from burns.stenotrophomonas maltophilia is rarely implicated in clinical infections but it constitutes a significant nosocomial pathogen, especially in immunocompromised patients. this report describes the first case of a generalised infection caused by s. maltophilia that included bacteremia, wound and respiratory tract infection in a patient suffering from burns. given the emergence of s. maltophilia nosocomial infections, especially in patients with burns, isolation of the bacterium from blood cultures ...200314556730
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2001--ii. gram-negative bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2001 were yearly evaluated and compared with those of other cephems, oxacephems and carbapenems. a total of 3,245 strains in 32 species of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis, escherichia coli, citrobacter freundii, citrobact ...200314567255
stenotrophomonas maltophilia contamination of nebulizers used to deliver aerosolized therapy to inpatients with cystic fibrosis.there is circumstantial evidence that nebulizer equipment may be a source of stenotrophomonas maltophilia for patients with cystic fibrosis. eighty-nine inpatient nebulizers were examined for evidence of s. maltophilia contamination of which nine (10%) yielded 14 strains of the bacterium. environmental samples were obtained from 73 different sites on the ward, of which 17 (23%) yielded a further 21 strains. positive sites included taps, sink drains, and potable water. genotyping using eric-pcr a ...200314572484
metal binding asp-120 in metallo-beta-lactamase l1 from stenotrophomonas maltophilia plays a crucial role in catalysis.metallo-beta-lactamase l1 from stenotrophomonas maltophilia is a dinuclear zn(ii) enzyme that contains a metal-binding aspartic acid in a position to potentially play an important role in catalysis. the presence of this metal-binding aspartic acid appears to be common to most dinuclear, metal-containing, hydrolytic enzymes; particularly those with a beta-lactamase fold. in an effort to probe the catalytic and metal-binding role of asp-120 in l1, three site-directed mutants (d120c, d120n, and d12 ...200414573595
[necrotizing otitis externa caused by stenotrophomonas maltophilia].the gram-negative bacterium stenotrophomonas maltophilia is feared, especially in intensive care wards, as a nosocomial opportunistic pathogen causing fulminant septicemia and organ failure in immunosuppressed patients. a patient with chronic lymphocytic leukemia developed a cutaneous infection with the clinical pattern of necrotizing otitis externa.200314593466
characterization of a soil-derived bacterial consortium degrading 4-chloroaniline.a bacterial consortium comprising four different species was isolated from an indonesian agricultural soil using a mixture of aniline and 4-chloroaniline (4ca) as principal carbon sources. the four species were identified as chryseobacterium indologenes sb1, comamonas testosteroni sb2, pseudomonas corrugata sb4 and stenotrophomonas maltophilia sb5. growth studies on aniline and 4ca as single and mixed substrates demonstrated that the bacteria preferred to grow on and utilize aniline rather than ...200314600240
spread of stenotrophomonas maltophilia colonization in a pediatric intensive care unit detected by monitoring tracheal bacterial carriage and molecular typing.in our pediatric intensive care unit in tours (france), intubated and ventilated inpatients are systematically monitored for tracheal bacterial colonization twice a week. this led us to detect five patients colonized with stenotrophomonas maltophilia over a 4-month period. molecular typing of the isolates using random amplified polymorphism dna (rapd) and pulsed-field gel electrophoresis (pfge) confirmed that four of the five isolates were genetically related. the strict isolation of carriers an ...200314616735
false-positive result of sterility testing after a supposed adverse transfusion reaction.we report on the case of a woman who suffered from chills and fever when she was transfused with two units of red blood cell concentrate. immunohaematological evaluation of the adverse reaction was inconspicuous, as well as sterility testing of one unit, but bacteriological evaluation of the second unit showed growth of stenotrophomonas maltophilia. the sample for sterility testing of this unit had been drawn from the distal end of the transfusion device that was still connected to the blood bag ...200314617343
endoscopic treatment in a patient with obstructive jaundice caused by pancreatic pseudocyst.we report a case of chronic alcoholic pancreatitis with obstructive jaundice due to compression of the common bile duct by pancreatic pseudocyst. ultrasonography and computed tomographic scan on admission demonstrated an 8 cm cystic lesion located at the head of the pancreas. endoscopic retrograde cholangiopancreatography (ercp) revealed a segment of narrowing at the distal common bile duct which was compressed by the pancreatic pseudocyst. communication between the cyst and pancreatic duct was ...200314649681
contemporary in vitro spectrum of activity summary for antimicrobial agents tested against 18569 strains non-fermentative gram-negative bacilli isolated in the sentry antimicrobial surveillance program (1997-2001).the frequency of occurrence and antimicrobial susceptibility patterns of 18569 non-fermentative gram-negative bacilli consecutively collected as part of the sentry antimicrobial surveillance program were summarized. the isolates were tested by the broth microdilution method in three coordinator laboratories using common reagents and reference methodologies. the most frequently isolated pathogen was pseudomonas aeruginosa (11968 isolates; 64.5%) followed by acinetobacter spp. (3468 isolates; 18.7 ...200314659650
the indirect pathogenicity of stenotrophomonas maltophilia.stenotrophomonas maltophilia has at least two inducible beta-lactamases, l1 and l2, which can hydrolyze almost all classes of beta-lactam antimicrobial agents. this study was done to verify the indirect pathogenicity of s. maltophilia that could promote the growth of other beta-lactam agent-susceptible bacteria in a mixed culture. we counted cfu of beta-lactam agent-susceptible bacteria under the presence of imipenem or ceftazidime in a pure culture and mixed culture with s. maltophilia. our res ...200314659658
lipid peroxidation of lung surfactant due to reactive oxygen species released from phagocytes stimulated by bacteria from children with cystic fibrosis.we used pseudomonas aeruginosa, burkholderia cepacia and stenotrophomonas maltophilia, live or heat-killed, isolated from the airways of children with cystic fibrosis, to stimulate human neutrophils (pmn) and rat alveolar macrophages (am) to produce reactive oxygen metabolites in the presence or absence of curosurf, a natural porcine lung surfactant. we determined: (1) the amount of lipid peroxidation (lpo) as assessed by the amounts of malondialdehyde (mda) and 4-hydroxyalkenals (4-hne) using t ...200314669998
bacteraemia in hospitalised patients with malignant blood disorders: a retrospective study of causative agents and their resistance profiles during a 14-year period without antibacterial prophylaxis.bacteraemia is a major cause of morbidity and mortality in patients with haematological disorders during chemotherapy-induced neutropenia. the generally reported trend during the last two decades has been a gradual replacement of gram-negative bacilli by gram-positive cocci as the major causes of bacteraemia in neutropenic hosts. however, data that are unaffected by the use of antibacterial prophylaxis are scarce. our objective therefore was to study the incidence of bacteraemia with different m ...200314671614
[pathogenic bacteria in cystic fibrosis].since the cf gene identification in 1989 and despite the improvement of our knowledge in the physiopathology of the disease, bronchopulmonary infection determines the vital prognosis. following staphylococcus aureus infection, patients are colonized or colonized by pseudomonas aeruginosa, greatly involved in the pulmonary deterioration. other bacteria may be involved burkholderia cepacia, stenotrophomonas maltophilia, alcaligenes sp. intensive antibiotic treatment of primocolonisation helps to p ...200314671932
exposure to bioaerosols in a municipal sewage treatment plant.microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ...200314677919
hospital-acquired pneumonia: coverage and treatment adequacy of current guidelines.the american thoracic society (ats) guideline for hospital-acquired pneumonia (hap) released in 1996 and the trouillet classification published in 1998 supply different rational foundations for the classification of patients with hap and for the selection of initial antibiotic therapy. the aims of this study were to assess the level of bacterial coverage and to assess and validate the adequacy of antibiotic strategy of each of these classifications. intensive care unit-admitted patients (n=71) w ...200314680072
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, monobactams, and penicillins. changes in czop susceptibility among bacteria were also evaluated with the bacterial resistance ratio calculated from the breakpoint mic. twenty-five species (4,154 strains) of gram-negative bacteria were isolated from the clinical ...200314692381
biofilm formation by stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime.we investigated the in vitro effects of seven fluoroquinolones (ciprofloxacin, grepafloxacin, levofloxacin, moxifloxacin, norfloxacin, ofloxacin, and rufloxacin), compared to those of trimethoprim-sulfamethoxazole (sxt) and ceftazidime on total biomass and cell viability of stenotrophomonas maltophilia biofilm. s. maltophilia attached rapidly to polystyrene, within 2 h of incubation, and then biofilm formation increased over time, reaching maximum growth at 24 h. in the presence of fluoroquinolo ...200414693533
antimicrobial susceptibility and synergy studies of stenotrophomonas maltophilia isolates from patients with cystic fibrosis.stenotrophomonas maltophilia is a newly emerging pathogen being detected with increasing frequency in patients with cystic fibrosis (cf). the impact of this multidrug-resistant organism on lung function is uncertain. the optimal treatment for s. maltophilia in cf patients is unknown. we studied the in vitro activity of ten antimicrobial agents, and conducted synergy studies by using checkerboard dilutions of eight pairs of antimicrobial agents against strains isolated from 673 cf patients from 1 ...200414693535
efflux-mediated multiresistance in gram-negative bacteria.multiresistance in gram-negative pathogens, particularly pseudomonas aeruginosa, stenotrophomonas maltophilia, acinetobacter spp. and the enterobacteriaceae, is a significant problem in medicine today. while multiple mechanisms often contribute to multiresistance, a broadly distributed family of three-component multidrug efflux systems is an increasingly recognised determinant of both intrinsic and acquired multiresistance in these organisms. homologues of these efflux systems are also readily i ...200414706082
antibiotic treatment of multidrug-resistant organisms in cystic fibrosis.respiratory tract infection with eventual respiratory failure is the major cause of morbidity and mortality in cystic fibrosis (cf). infective exacerbations need to be treated promptly and effectively to minimize potentially accelerated attrition of lung function. the choice of antibiotic depends on in vitro sensitivity patterns. however, physicians treating patients with cf are increasingly faced with infection with multidrug-resistant isolates of pseudomonas aeruginosa. in addition, innately r ...200314719998
implantable venous port-related infections in cancer patients.to evaluate the characteristics of implantable venous port-related infections in patients with malignancies.200414727170
case-control study of stenotrophomonas maltophilia acquisition in cystic fibrosis patients.the aims of this case-control study were to describe the characteristics of cystic fibrosis (cf) patients who isolated stenotrophomonas maltophilia in sputum, to determine risk factors for acquisition, to assess persistence of the organism and clinical outcomes postacquisition. data were collected from 1991-1999. cf patients and controls (who had never isolated s. maltophilia) were matched for age (+/- 1 yr), sex and forced expiratory volume in one second (+/- 10%). data were collected from the ...200414738239
overexpression of the multidrug efflux pump smedef impairs stenotrophomonas maltophilia physiology.the use of antibiotics for the treatment of infectious diseases has led to important changes in the structure of pathogenic bacterial populations. however, these changes could be buffered if the expression of antibiotic resistance genes were to lead to the counter-selection of antibiotic-resistant strains in antibiotic-free environments. to test the effect of antibiotic resistance on bacterial fitness, we analysed the effect of the overproduction of the multidrug efflux pump smedef on the physio ...200414739147
class 1 integrons increase trimethoprim-sulfamethoxazole mics against epidemiologically unrelated stenotrophomonas maltophilia isolates.twenty-five plasmid-specified antimicrobial resistance determinants common to gram-negative bacilli from nosocomial infection were investigated from 31 stenotrophomonas maltophilia isolates. twenty-four clones were identified by pulsed-field gel electrophoresis, and in three clones that exhibited an increased trimethoprim-sulfamethoxazole mic, the sul1 determinant was found. these results support not only the higher spread of class 1 integrons compared to other mechanisms but also the potential ...200414742234
contribution of integrons, and smeabc and smedef efflux pumps to multidrug resistance in clinical isolates of stenotrophomonas maltophilia.the contribution of integrons and efflux pumps to multidrug resistance in stenotrophomonas maltophilia was evaluated.200414749340
biodegradation of dna and nucleotides to nucleosides and free bases.thirty-two different microorganisms were examined in order to check their ability to degrade an exogenous dna. bacteria from species: stenotrophomonas maltophilia, brevundimonas diminuta, bacillus subtilis, mycobacterium butyricum and fungus fusarium moniliforme were capable to degrade dna to nucleic bases or their derivatives. degradation of dna by s. maltophilia resulted in formation of free bases, such as hypoxanthine, thymine, uracil and xanthine. the optimum concentration of dna seemed to b ...200414751311
synthesis, and antiprotozoal and antibacterial activities of s-substituted 4,6-dibromo- and 4,6-dichloro-2-mercaptobenzimidazoles.the synthesis and some germicidal activities in vitro of two congener series of s-substituted 4,6-dihalogeno-2-mercapto-1h-benzimidazoles are reported. there was no substantial difference between antibacterial activities of corresponding 4,6-dichloro- and 4,6-dibromo-derivatives. the present results confirm lower susceptibility to substituted benzimidazoles of gram-negative compared to gram-positive bacteria. minimum inhibitory concentrations (mics) of a majority of the novel derivatives ranged ...200414757505
high genetic diversity among stenotrophomonas maltophilia strains despite their originating at a single hospital.the levels of genetic relatedness of 139 stenotrophomonas maltophilia strains recovered from 105 hospitalized non-cystic fibrosis patients (51% from medical wards, 35% from intensive care units, and 14% from surgical wards) and 7 environmental sources in the same hospital setting during a 4-year period were typed by the pulsed-field gel electrophoresis (pfge) technique. a total of 99 well-defined distinct xbai pfge patterns were identified (simpson's discrimination index, 0.996). the dendrogram ...200414766838
epidemiology of nosocomial pneumonia in infants after cardiac surgery.the pattern of nosocomial pneumonia (np) in infants in a pediatric surgical icu after cardiac surgery may differ from that seen in adult icus.200414769717
[corneal infiltrates and ulcers. a retrospective study of 239 eyes].in our outpatient service the number of corneal infiltrates and ulcers associated with contact lens wear in young patients increased during the last years. therefore we evaluated all patients with keratitis regarding to the reason.200414872265
in vitro susceptibility of stenotrophomonas maltophilia isolates: comparison of disc diffusion, etest and agar dilution methods.the disc diffusion, etest and agar dilution techniques were compared to evaluate the antimicrobial susceptibility profile of 70 stenotrophomonas maltophilia isolates to seven antimicrobial agents. the s. maltophilia isolates were consecutively collected from may 2000 to may 2002 from individual patients, who were hospitalized in a private brazilian hospital. the antimicrobial susceptibility tests were carried out and interpreted according to the national committee for clinical laboratory standar ...200414973153
Displaying items 1001 - 1100 of 2648