Publications

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formation of wallal virus in cell culture. 19724677124
susceptibility of orbiviruses to low ph and to organic solvents.six orbiviruses, corriparta, wallal, eubenangee, d'aguilar, warrego and mitchell river, were inactivated within 30 min in solutions of ph less than 6. all but wallal virus resisted treatment with ether at 1 degrees for 22 h but only corriparta virus resisted treatment with chloroform at 1 degrees for 22 h. the genomes of the orbiviruses, including corriparta virus, after electrophoresis in acrylamide gels separated into basically similar patterns which were distinct from the pattern of reovirus ...197830445
detection of reassortant orbiviruses (wallal serogroup) in a prototype strain isolated from a pool of biting midges (culicoides dycei).genetic variation between clones selected from early passage pools of wallal virus (reoviridae, orbivirus) was investigated. the virus had been isolated in 1970 from a pool of 100 insects (culicoides dycei), caught in the wild, by laboratory passage in suckling mice. gel electrophoresis and oligonucleotide fingerprint analysis of clones indicated that multiple reassortant genotypes were present in early passages of the original isolate. the virus, previously described as the prototype strain of ...19892732705
epidemic of blindness in kangaroos - evidence of a viral aetiology.objective to determine the cause of an epidemic of blindness in kangaroos. design and procedures laboratory examinations were made of eyes and brains of a large number of kangaroos using serological, virological, histopathological, electron microscopical, immunohistochemical methods, and pcr with cdna sequencing. in addition, potential insect viral vectors identified during the disease outbreak were examined for specific viral genomic sequences. sample population for histopathological analysis, ...199928677135
experimental reproduction of viral chorioretinitis in kangaroos.to investigate whether preparations containing wallal and/or warrego viruses could cause disease when inoculated subcutaneously into captive kangaroos.199910494399
epidemic of blindness in kangaroos--evidence of a viral aetiology.to determine the cause of an epidemic of blindness in kangaroos. design and procedures: laboratory examinations were made of eyes and brains of a large number of kangaroos using serological, virological, histopathological, electron microscopical, immunohistochemical methods, and pcr with cdna sequencing. in addition, potential insect viral vectors identified during the disease outbreak were examined for specific viral genomic sequences.199910494400
immunohistochemistry in the identification of a number of new diseases in australia.immunohistochemistry plays an important part in the diagnosis of some viral diseases. demonstration of viral antigen in a lesion is an important contribution to diagnosis, either at the time of investigation or retrospectively. at the csiro australian animal health laboratory, the most frequent use of immunohistochemistry has been in the diagnosis of the important avian diseases, highly pathogenic avian influenza and newcastle disease. the technology took key roles in the diagnoses of hendra vir ...199910501165
enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes.viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ...201324314645
characterization of a novel negevirus and a novel bunyavirus isolated from culex (culex) declarator mosquitoes in trinidad.pools of mosquitoes were tested for insect-specific viruses using cytopathic effect (cpe) assays on aedes albopictus (c6/36) cells. illumina sequencing of rna from pool tr7094, which produced extensive cpe 2 days post-infection, yielded the complete genome sequences of a previously unknown bunyavirus, designated cumuto virus (cumv), and a second virus designated wallerfield virus (walv). walv shared highest amino acid identity (60.1 %) with dezidougou virus from côte d'ivoire, a positive-sense, ...201324262627
full genome characterization of the culicoides-borne marsupial orbiviruses: wallal virus, mudjinbarry virus and warrego viruses.viruses belonging to the species wallal virus and warrego virus of the genus orbivirus were identified as causative agents of blindness in marsupials in australia during 1994/5. recent comparisons of nucleotide (nt) and amino acid (aa) sequences have provided a basis for the grouping and classification of orbivirus isolates. however, full-genome sequence data are not available for representatives of all orbivirus species. we report full-genome sequence data for three additional orbiviruses: wall ...201425299687
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