| characterization of veillonella atypica pk1910 adhesin-mediated coaggregation with oral streptococcus spp. | the gram-negative human oral bacterium veillonella atypica pk1910 exhibits both lactose-inhibitable and lactose-noninhibitable coaggregations with certain human oral streptococci. a mild sonication procedure was used to obtain a veillonella surface protein preparation against which antisera were prepared. to characterize the lactose-inhibitable coaggregation, coaggregation-defective (cog-) mutants unable to exhibit this kind of coaggregation (class 1 mutants) were used to absorb the antisera. on ... | 1992 | 1541534 |
| isolation and characterization of coaggregation-defective mutants of veillonella atypica. | spontaneously occurring coaggregation-defective mutants of veillonella atypica pk1910 were isolated and used to study the interactions of veillonella with streptococcus sanguis, strep. gordonii and strep. sm. three classes of mutants were isolated. the first class failed to participate in lactose-inhibitable coaggregations with streptococcal cells. the second class of mutants failed to coaggregate by the lactose-non-inhibitable interactions. the third group, isolated from the first group, failed ... | 1990 | 2088215 |
| coaggregation properties of human oral veillonella spp.: relationship to colonization site and oral ecology. | the primary habitats of oral veillonellae are the tongue, dental plaque, and the buccal mucosa. isolates were obtained from each habitat and tested for coaggregation with a battery of other oral bacterial strains. all 59 tongue isolates tested for coaggregation were veillonella atypica or veillonella dispar. all but one of them coaggregated with strains of streptococcus salivarius, a predominant inhabitant of the tongue surface but not subgingival dental plaque. these tongue isolates were unable ... | 1988 | 3178207 |
| characterization of inhibitory effects of suspected periodontopathogens on osteogenesis in vitro. | by using an in vitro bone-forming culture system, the chick periosteal osteogenesis (cpo) model, the direct effects on osteogenesis of sonicated extracts derived from oral bacteria were examined. both extracts from bacterial species having strong associations with periodontal diseases (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and prevotella intermedia, hereinafter referred to as suspected periodontopathogens) and extracts from species not correlated with periodontal diseas ... | 1995 | 7642257 |
| investigation of the influences of puberty, genetics, and environment on the composition of subgingival periodontal floras. | the classical twin model was utilized in this study in an attempt to determine the importance of host genetics to the composition of the subgingival flora. simultaneously, the effect of puberty on the flora composition was assessed. the compositions of the floras were significantly different at ages 11 and 14 in the same people, indicating that transition to an adult flora composition may be initiated during puberty. however, the numbers of subjects who had prepubertal and postpubertal testoster ... | 1993 | 8514392 |
| isolation and characterization of coaggregation-defective (cog-) mutants of streptococcus gordonii dl1 (challis). | streptococcus gordonii dl1 (challis) bears coaggregation-mediating surface adhesins which recognize galactoside-containing surface polysaccharides on streptococcus oralis 34, streptococcus oralis c104, and streptococcus sm pk509. fifty-nine spontaneously-occurring coaggregation-defective (cog-) mutants of s. gordonii dl1 unable to coaggregate with partner streptococci were isolated. six representative cog- mutants were characterized by their coaggregation properties with four actinomyces naeslun ... | 1995 | 8519477 |
| characterization of xanthine dehydrogenase from the anaerobic bacterium veillonella atypica and identification of a molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor. | the molybdenum-containing iron-sulfur flavoprotein xanthine dehydrogenase from the anaerobic bacterium veillonella atypica has been purified approximately 800-fold with a yield of approximately 40% and a specific activity of approximately 70 micromol ferricyanide reduced x min(-1) x mg protein(-1) with xanthine as electron donor, which corresponds to approximately 30 micromol xanthine oxidized x min(-1) x mg protein(-1) with methylene blue as electron acceptor. the 129-kda enzyme was a non-coval ... | 1996 | 8706691 |
| differentiation of veillonella atypica, veillonella dispar and veillonella parvula using restricted fragment-length polymorphism analysis of 16s rdna amplified by polymerase chain reaction. | veillonella atypica, veillonella dispar and veillonella parvula cannot be reliably distinguished by conventional phenotypic tests, including the api zym test. in this study, restricted fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction (pcr) was used to generate restriction profiles of the type strains of v. atypica, v. dispar and v. parvula and 20 veillonella strains isolated from oral sites. 16s rrna gene sequences from isolated genomic dna samples ... | 1997 | 9573809 |
| treponema denticola outer membrane enhances the phagocytosis of collagen-coated beads by gingival fibroblasts. | human gingival fibroblasts (hgfs) degrade collagen fibrils in physiological processes by phagocytosis. since treponema denticola outer membrane (om) extract perturbs actin filaments, important structures in phagocytosis, we determined whether the om affects collagen phagocytosis in vitro by hgfs. phagocytosis was measured by flow cytometric assessment of internalized collagen-coated fluorescent latex beads. confluent hgfs pretreated with t. denticola atcc 35405 om exhibited an increase in the pe ... | 1999 | 10024564 |
| intra-chromosomal heterogeneity between the four 16s rrna gene copies in the genus veillonella: implications for phylogeny and taxonomy. | among the seven species characterized within the genus veillonella, three (veillonella dispar, veillonella parvula and veillonella atypica) have so far been isolated from human flora and during infectious processes. sequencing and analysis of 16s rdna (rrs) has been described as the best method for identification of veillonella strains at the species level since phenotypic characteristics are unable to differentiate between species. rrs sequencing for the three species isolated from humans showe ... | 2003 | 12777489 |
| systematic 16s rrna gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans. | clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16s rrna gene sequence analysis. each isolate yielded a > or =1,400-bp sequence containing <5 ambiguities which was compared with the genbank 16s rrna gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (< or =10 cases reported in the literature) human pathogens. eleven new species, "actinobaculum massiliae," ... | 2004 | 15131188 |
| development of a multispecies oral bacterial community in a saliva-conditioned flow cell. | microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. we used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation ... | 2004 | 15240317 |
| interspecies communication in streptococcus gordonii-veillonella atypica biofilms: signaling in flow conditions requires juxtaposition. | during the development of human oral biofilm communities, the spatial arrangement of the bacteria is thought to be driven by metabolic interactions between them. streptococcus gordonii and veillonella atypica, two early colonizing members of the dental plaque biofilm, have been postulated to participate in metabolic communication; s. gordonii ferments carbohydrates to form lactic acid, which is a preferred fermentation substrate for v. atypica. we found that, during agar-plate coculture of these ... | 2004 | 15546975 |
| evaluation of bacterial nitrate reduction in the human oral cavity. | it is hypothesized that the enterosalivary nitrate circulation encourages nitrate reducing bacteria to reside within the oral cavity. nitrite production may then limit the growth of acidogenic bacteria as a result of the production of antimicrobial oxides of nitrogen, including nitric oxide. this study was carried out with 10 subjects to characterize oral nitrate reduction and identify the bacteria responsible. nitrate reduction varied between individuals (mean 85.4 +/- 15.9 nmol nitrite min(-1) ... | 2005 | 15693824 |
| molecular identification of the first human isolate belonging to the veillonella ratti-veillonella criceti group based on 16s rdna and dnak gene sequencing. | anaerobic gram-negative cocci belonging to the genus veillonella are currently subdivided into eight species. among them, four have been isolated either from human flora or from clinical samples during infectious processes: veillonella atypica, v. dispar, v. parvula and v. montpellierensis. to date, the four other species have only been found in animals. in particular, v. ratti and v. criceti, since their characterization, have been exclusively reported from mouth and intestine of rodents. we re ... | 2005 | 15862461 |
| the genus veillonella iv. serological groupings, and genus and species emendations. | rogosa, m. (national institute of dental research, bethesda, md.). the genus veillonella. iv. serological groupings, and genus and species emendations. j. bacteriol. 90:704-709. 1965.-seven serological groups of veillonella were found. the generic and species descriptions are enlarged and corrected. the type species is veillonella parvula (veillon and zuber) prévot, 1933. the proposed species, subspecies (infraspecific serogroups), and american type culture collection (atcc) type strains are: ve ... | 1965 | 16562069 |
| molecular identification of bacteria on the tongue dorsum of subjects with and without halitosis. | compare the microbial profiles on the tongue dorsum in patients with halitosis and control subjects in a uk population using culture-independent techniques. | 2008 | 18336372 |
| the predominant cultivable veillonella spp. of the tongue of healthy adults identified using rpob sequencing. | the predominant veillonella spp. were isolated from the dorsum surface of the tongues of 11 healthy adults and identified to species level using rpob sequencing because 16s ribosomal rna sequence analysis does not reliably differentiate between all members of this genus. in all, 253 isolates were identified and the mean proportion (+/- se) of veillonella spp. per sample was 16.2 (+/- 3.6) with a range of 3.0% to 36.3% of the total anaerobic colony count. the predominant species were veillonella ... | 2008 | 18582335 |
| interspecies signaling between veillonella atypica and streptococcus gordonii requires the transcription factor ccpa. | streptococcus gordonii and veillonella atypica, two early-colonizing members of the dental plaque biofilm, participate in a relationship that results in increased transcription of the s. gordonii gene amyb, encoding an alpha-amylase. we show that the transcription factor ccpa is required for this interspecies interaction. | 2009 | 19542285 |
| identification of oral species of the genus veillonella by polymerase chain reaction. | members of the genus veillonella cannot be reliably distinguished by their biochemical characteristics and phenotypic features. moreover, dna-dna hybridization and sequence analyses of the 16s ribosomal rna gene including random fragment length polymorphism analysis, are complex and time-consuming procedures that are not well-suited to identifying oral species of veillonella: veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, and veillonella rogosae. | 2009 | 19572893 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| investigating different mechanisms for biogenic selenite transformations: geobacter sulfurreducens, shewanella oneidensis and veillonella atypica. | the metal-reducing bacteria geobacter sulfurreducens, shewanella oneidensis and veillonella atypica, use different mechanisms to transform toxic, bioavailable sodium selenite to less toxic, non-mobile elemental selenium and then to selenide in anaerobic environments, offering the potential for in situ and ex situ bioremediation of contaminated soils, sediments, industrial effluents, and agricultural drainage waters. the products of these reductive transformations depend on both the organism invo ... | 2009 | 19950474 |
| microbial manufacture of chalcogenide-based nanoparticles via the reduction of selenite using veillonella atypica: an in situ exafs study. | the ability of metal-reducing bacteria to produce nanoparticles, and their precursors, can be harnessed for the biological manufacture of fluorescent, semiconducting nanomaterials. the anaerobic bacterium veillonella atypica can reduce selenium oxyanions to form nanospheres of elemental selenium. these selenium nanospheres are then further reduced by the bacterium to form reactive selenide which could be precipitated with a suitable metal cation to produce nanoscale chalcogenide precipitates, su ... | 2008 | 21825617 |
| effects of ph and lactate on hydrogen sulfide production by oral veillonella spp. | indigenous oral bacteria in the tongue coating such as veillonella have been identified as the main producers of hydrogen sulfide (h2s), one of the major components of oral malodor. however, there is little information on the physiological properties of h2s production by oral veillonella such as metabolic activity and oral environmental factors which may affect h2s production. thus, in the present study, the h2s-producing activity of growing cells, resting cells, and cell extracts of oral veillo ... | 2014 | 24795374 |
| development of in vitro denture biofilm models for halitosis related bacteria and their application in testing the efficacy of antimicrobial agents. | since dentures can serve as a reservoir for halitosis-causing oral bacteria, halitosis development is a concern for denture wearers. in this study, we surveyed the prevalence of four selected halitosis-related species (fusobacterium nucleatum, tannerella forsythia, veillonella atypica and klebsiella pneumoniae) in clinical denture plaque samples, and developed denture biofilm models for these species in vitro to facilitate assessment of antimicrobial treatment efficacy. design : denture plaque f ... | 2015 | 25926895 |
| development and pyrosequencing analysis of an in-vitro oral biofilm model. | dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. the principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. in-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. the aim of this study was to develop an oral biofilm model using the calgary biofilm device (cb ... | 2015 | 25880819 |
| a yada-like autotransporter, hag1 in veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, porphyromonas gingivalis, and human oral buccal cells. | dental biofilm development is a sequential process, and adherence between microbes and the salivary pellicle (adhesion) as well as among different microbes (co-adhesion or coaggregation) plays a critical role in building a biofilm community. the veillonella species are among the most predominant species in the oral cavity and coaggregate with many initial, early, middle, and late colonizers. similar to oral fusobacteria, they are also considered bridging species in biofilm development. however, ... | 2015 | 25440509 |
| altered bacterial profiles in saliva from adults with caries lesions: a case-cohort study. | the aim of this study was to learn whether presence of caries in an adult population was associated with a salivary bacterial profile different from that of individuals without untreated caries. stimulated saliva samples from 621 participants of the danish health examination survey were analyzed using the human oral microbe identification microarray technology. samples from 174 individuals with dental caries and 447 from a control cohort were compared using frequency and levels of identified bac ... | 2014 | 24643218 |
| nitrate reductase activity of bacteria in saliva of term and preterm infants. | the salivary glands of adults concentrate nitrate from plasma into saliva where it is converted to nitrite by bacterial nitrate reductases. nitrite can play a beneficial role in adult gastrointestinal and cardiovascular physiology. when nitrite is swallowed, some of it is converted to nitric oxide (no) in the stomach and may then exert protective effects in the gastrointestinal tract and throughout the body. it has yet to be determined either when newborn infants acquire oral nitrate reducing ba ... | 2012 | 22842223 |
| regulation of adrenomedullin and nitric oxide production by periodontal bacteria. | in periodontitis the host response to bacterial challenge includes activity of the multifunctional molecules adrenomedullin (am) and nitric oxide (no). the aim of this study was to investigate the role of periodontal bacteria in regulating the production of these molecules from cultured cells. | 2015 | 25440112 |
| body fluid identification by integrated analysis of dna methylation and body fluid-specific microbial dna. | identification of body fluids found at crime scenes provides important information that can support a link between sample donors and actual criminal acts. previous studies have reported that dna methylation analysis at several tissue-specific differentially methylated regions (tdmrs) enables successful identification of semen, and the detection of certain bacterial dna can allow for identification of saliva and vaginal fluid. in the present study, a method for detecting bacterial dna was integra ... | 2014 | 24052059 |
| identification and characterization of a haem biosynthesis locus in veillonella. | haemin/haem is one of the essential nutrients required by periodontopathogens such as porphyromonas gingivalis to grow in vitro. in the oral cavity, this nutrient is believed to be provided by the crevicular fluid, a serum-like exudate produced during gum inflammation. however, p. gingivalis is also present in the healthy dental biofilm where inflammation is absent. this study was designed to answer the question: what organism(s) in the healthy dental biofilm provides haemin/haem to those period ... | 2016 | 27566661 |
| microbiological and bioinformatics analysis of primary sjogren's syndrome patients with normal salivation. | reduced salivation is considered a major clinical feature of most but not all cases of primary sjögren's syndrome (pss). reduced saliva flow may lead to changes in the salivary microbiota. these changes have mainly been studied with culture that typically recovers only 65% of the bacteria present. | 2016 | 27770517 |
| the influence of oral veillonella species on biofilms formed by streptococcus species. | oral veillonella, veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, veillonella rogosae, and veillonella tobetsuensis are known as early colonizers in oral biofilm formation. to investigate the role of oral veillonella, biofilms formed by the co-culture of streptococcus gordonii, streptococcus mutans, streptococcus salivarius, or streptococcus sanguinis, with oral veillonella were examined at the species level. the amount of biofilm formed by s. mutans, s. g ... | 2014 | 24862495 |
| establishment of a counter-selectable markerless mutagenesis system in veillonella atypica. | using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the veillonella genus. this system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in veillonella. | 2015 | 25771833 |
| effects of short-term xylitol gum chewing on the oral microbiome. | the aim of this study was to determine the effects of short-term xylitol gum chewing on the salivary microbiota of children. | 2015 | 24663814 |
| identification of veillonella tobetsuensis in tongue biofilm by using a species-specific primer pair. | veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, and veillonella rogosae have been reported to be isolated from human oral cavities. the recently detected veillonellatobetsuensis in human tongue biofilms was proposed as a novel veillonella sp. in this study, to determine the distribution and frequency of v. tobetsuensis, we established a method for the detection and identification of v. tobetsuensis by using polymerase chain reaction (pcr) using a species-s ... | 2013 | 23664905 |
| ex situ formation of metal selenide quantum dots using bacterially derived selenide precursors. | luminescent quantum dots were synthesized using bacterially derived selenide (se(ii-)) as the precursor. biogenic se(ii-) was produced by the reduction of se(iv) by veillonella atypica and compared directly against borohydride-reduced se(iv) for the production of glutathione-stabilized cdse and β-mercaptoethanol-stabilized znse nanoparticles by aqueous synthesis. biological se(ii-) formed smaller, narrower size distributed qds under the same conditions. the growth kinetics of biologically source ... | 2013 | 23508116 |