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antagonistic and plant growth activity of trichoderma isolates of western himalayas.the genus trichoderma is rapidly growing colonies bearing tufted or postulate, repeatedly branched conidiophores with lageniform phialides and hyaline or green conidia born in slimy heads. 62 isolates of trichoderma species were isolated from different rhizospheric soil samples collected from different places located in western himalayas region. out of these only two species were found i.e. trichoderma hazianum and trichoderma viride. their efficacy against soil borne plant pathogens like sclero ...201021506476
superoxide radical is involved in the sclerotial differentiation of filamentous phytopathogenic fungi: identification of a fungal xanthine oxidase.this study shows that the direct indicator of oxidative stress superoxide radical (o·₂⁻) is involved in the sclerotial differentiation of the phytopathogenic filamentous fungi rhizoctonia solani, sclerotinia sclerotiorum, sclerotium rolfsii, and sclerotinia minor. the production rate of o·₂⁻ and the antioxidant enzyme superoxide dismutase (sod) levels in the sclerotiogenic fungi were significantly higher and lower, respectively, than those of their non-differentiating counterpart strains, which ...201020943149
polygalacturonase inhibitor protein from fruits of anthracnose resistant and susceptible varieties of chilli (capsicum annuum l).chilli fruit is highly susceptible to anthracnose infection at the stage of harvest maturity, due to which the fruit yield in the leading commercial variety byadgi is severely affected. field studies on screening of several varieties for resistance to anthracnose have shown that a variety of chilli ar-4/99k is resistant to anthracnose infection. in many crops, resistance to fungal attack has been correlated with pgip activity in developing fruits based on which transgenic varieties have been dev ...201021174952
synthesis and bioefficacy evaluation of new 3-substituted-3,4-dihydro-1,3-benzoxazines.a new series of 1, 3-benzoxazines were synthesized, characterized ((1)h nmr and (13)c nmr) and evaluated for their pesticidal activity. six new 3-alkyl-3, 4-dihydro-4-methyl-2h-1, 3-benzoxazines (1-6) were prepared by hydroxymethylation of secondary amines with formaldehyde in 65-68% yields. these compounds were screened for there igr activity against spodoptera litura and for antifungal fungal activity in vitro against sclerotium rolfsii itcc 6181 by poisoned food technique. insect growth regul ...201020390939
transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer sclerotium rolfsii.the plant pathogenic basidiomycete sclerotium rolfsii produces the industrially exploited exopolysaccharide scleroglucan, a polymer that consists of (1 --> 3)-beta-linked glucose with a (1 --> 6)-beta-glycosyl branch on every third unit. although the physicochemical properties of scleroglucan are well understood, almost nothing is known about the genetics of scleroglucan biosynthesis. similarly, the biosynthetic pathway of oxalate, the main by-product during scleroglucan production, has not been ...201020504312
microwave synthesis and antifungal evaluations of some chalcones and their derived diaryl-cyclohexenones.microwave irradiation (mwi) of acetophenones and substituted benzaldehydes in water resulted in a "green-chemistry" procedure for the preparation of chalcones (1-14), through base catalyzed claisen-schmidt condensation reaction, in good yields. further 3,5-diaryl-6-carbethoxy-2-cyclohexen-1-ones (1a-14a) were prepared through base catalyzed cyclocondensation of above chalcones with ethylacetoacetate using mwi as the energy source and silica as support. out of fourteen cyclohexenones, ten (1a, 4a ...201020574873
structure-biological activity relationships in triterpenic saponins: the relative activity of protobassic acid and its derivatives against plant pathogenic fungi.triterpenic saponins from sapindus mukorossi gaertn. and diploknema butyracea jf gmelin were evaluated for in vitro antifungal activity against four phytopathogenic fungi. the study of the structure-antifungal activity relationships of protobassic acid saponins was widened by including semi-synthetic derivatives.201020603877
overlapping and distinct functions of two trichoderma virens map kinases in cell-wall integrity, antagonistic properties and repression of conidiation.we have studied the functions of the trichoderma virens tmkb, a homologue of the yeast cell-wall integrity map kinase slt2, using gene knockout. the functions of tmkb were compared to those of the pathogenicity map kinase homologue (tmka). like the tmka loss-of-function mutants, tmkb mutants exhibited reduced radial growth and constitutive conidiation in dark as well as in liquid shake cultures. the tmkb mutants, in contrast to tmka mutants, had cell-wall integrity defects, as shown by autolysis ...201020633533
hydrogen peroxide is involved in the sclerotial differentiation of filamentous phytopathogenic fungi.the purpose of this study was to investigate the role of h(2) o(2) and the related oxidative stress markers catalase (cat) and lipid peroxidation in the sclerotial differentiation of the phytopathogenic filamentous fungi sclerotium rolfsii, sclerotinia minor, sclerotinia sclerotiorum and rhizoctonia solani.201020681971
effect of abiotic stress on phosphate solubilization by biocontrol fungus trichoderma sp.this study was undertaken to explore the role of trichoderma sp. in phosphate (p) solubilization and antagonism against fungal phytopathogens. all fungal isolates (se(6), kt(6), kt(28), and brt(11)) and a standard culture of t. harzianum (th-std) were able to antagonize two fungal phytopathogens (sclerotium rolfsii and rhizoctonia solani) of chickpea (cicer arietinum l.) wilt complex. transmission electron microscopic studies (tem) further confirmed ultra-cytological changes in the sclerotia of ...201121327557
exquisite binding specificity of sclerotium rolfsii lectin toward tf-related o-linked mucin-type glycans.sclerotium rolfsii lectin (srl), a secretory protein from the soil borne phytopathogenic fungus sclerotium rolfsii, has shown in our previous studies to bind strongly to the oncofetal thomson-friedenreich carbohydrate (galß1-3galnac-ser/thr, t or tf) antigen. tf antigen is widely expressed in many types of human cancers and the strong binding of srl toward such a cancer-associated carbohydrate structure led us to characterize the carbohydrate binding specificity of srl. glycan array analysis, wh ...201121347715
isolation, characterization and antifungal activity of major constituents of the himalayan lichen parmelia reticulata tayl.antifungal activity of hexane, ethyl acetate and methanol extracts of parmelia reticulata was evaluated against soilborne pathogenic fungi, namely, sclerotium rolfsii, rhizoctonia solani, r. bataticola, fusarium udum, pythium aphanidermatum and p. debaryanum by poisoned food technique. maximum antifungal activity was exhibited by hexane and ethyl acetate extracts against most of the test pathogens. secondary metabolites, namely, (±)-isousnic acid, (±)-protolichesterinic acid, atranorin, evernyl, ...201121351753
scleroglucan: biosynthesis, production and application of a versatile hydrocolloid.since its first description in the early 1960s, scleroglucan attracted much attention from both academia and industry. scleroglucan is an exopolysaccharide secreted by the basidiomycete sclerotium rolfsii and appreciated as a multipurpose compound applicable in many industrial fields, including oil industry, food industry and pharmacy. in this review, the current knowledge on scleroglucan chemistry, genetics, biosynthesis and production will be summarized and different application possibilities ...201121732244
purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of ophioglossum pedunculosum.a novel mannan-specific lectin was isolated from the roots of a traditional chinese herbal medicine, ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. with a molecular mass of 19,835.7 da demonstrated by maldi-tof analysis, this novel agglutinin was designated as o. pedunculosum agglutinin (opa), specifically agglutinating human o erythrocytes and rabbit erythrocytes. the hemagglutination could be strongly inhibited by mannan and thyroglobulin, the activity of whi ...201121947760
Involvement of phenazines and lipopeptides in interactions between Pseudomonas species and Sclerotium rolfsii, causal agent of stem rot disease on groundnut.Aims: To determine the role of phenazines (PHZ) and lipopeptide surfactants (LPs) produced by Pseudomonas in suppression of stem rot disease of groundnut, caused by the fungal pathogen Sclerotium rolfsii. Methods and Results: In vitro assays showed that PHZ-producing Pseudomonas chlororaphis strain Phz24 significantly inhibited hyphal growth of S. rolfsii and suppressed stem rot disease of groundnut under field conditions. Biosynthesis and regulatory mutants of Phz24 deficient in PHZ productio ...201122121884
Bioassays guided fractionation of Coronopus didymus for its antifungal activity against Sclerotium rolfsii.Sclerotium rolfsii Sacc. is a pathogen of about 500 plant species. In a laboratory screening bioassay, methanolic extracts of 15?mg?mL(-1) concentrations of different parts of Coronopus didymus (L.) Sm. (Brassicaceae) namely leaf, stem, inflorescence and root reduced the biomass of S. rolfsii by 67%, 26%, 40% and 58%, respectively. Methanolic root extract was successively fractionated with n-hexane, chloroform, ethyl acetate and n-butanol. All the concentrations (3.125-200?mg?mL(-1)) of ethyl ac ...201122007784
Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products.Tetrabromobisphenol A (TBBPA) degradation was investigated using white rot fungi and their oxidative enzymes. Strains of the Trametes, Pleurotus, Bjerkandera and Dichomitus genera eliminated almost 1 mM TBBPA within 4 days. Laccase, whose role in TBBPA degradation was demonstrated in fungal cultures, was applied to TBBPA degradation alone and in combination with cellobiose dehydrogenase from Sclerotium rolfsii. Purified laccase from Trametes versicolor degraded approximately 2 mM TBBPA within 5 ...201121865031
the antifungal constituents from the seeds of itoa orientalis.three new phenolic constituents, itolide a (1), itolide b (2), itoside p (3), and 1d-3-deoxy-3-hydroxymethyl-myo-inositol (4), which is described herein for the first time as a natural product, were isolated along with four other known compounds (5 to 8) from the methanol extract of the seeds of itoa orientalis hemsl by the activity-guided fractionation. their structures were determined by spectroscopic means. compounds 1 to 8 exhibited antifungal activities against sclerotium rolfsii with ic(50 ...201122233862
isolation and characterization of trichoderma spp. for antagonistic activity against root rot and foliar pathogens.trichoderma, soil-borne filamentous fungi, are capable of parasitising several plant pathogenic fungi. twelve isolates of trichoderma spp. isolated from different locations of south andaman were characterized for their cultural, morphological and antagonistic activity against soil borne and foliar borne pathogens. the sequencing of these isolates showed seven different species. the isolates revealed differential reaction patterns against the test pathogens viz., sclerotium rolfsii, colletotrichu ...201123729873
occurrence of sclerotium rot in allium tuberosum caused by sclerotium rolfsii in korea.in this study, we characterized sporadically occurring sclerotium rot caused by sclerotium rolfsii in chinese chive (allium tuberosum roth.) in farm fields in sacheon, korea. the initial symptom of the disease was water-soaked, which progressed to rotting, wilting, blighting, and eventually death. further, mycelial mats spread over the lesions near the soil line, and sclerotia formed on the scaly stem and leaves. the sclerotia were globoid, 1~3 mm, and white to brown. the optimum temperature for ...201122783108
first report of stem rot on asiatic dayflower (commelina communis l.) caused by sclerotium rolfsii in korea.stem rot was found for the first time on the asiatic dayflower plant (commelina communis l.) in korea. a detailed description of this korean specimen is given, along with its rdna internal transcribed spacer sequence. the fungus was identified as sclerotium rolfsii saccardo based on mycological characteristics and molecular data.201122783075
biological control of chickpea collar rot by co-inoculation of antagonistic bacteria and compatible rhizobia.two hundred and seven bacteria were isolated from composts and macrofauna and screened for plant growth promoting and antagonistic traits. seven of the 207 isolates showed antagonistic activity against sclerotium rolfsii in plate culture. inhibition of s. rolfsii by the bacterial isolates ranged between 61 and 84%. two of the seven isolates were bacillus sp. and rest belonged to pseudomonas sp. two isolates, pseudomonas sp. cdb 35 and pseudomonas sp. bwb 21 was compatible with chickpea rhizobium ...201122282609
characterization of rhizosphere bacteria for control of phytopathogenic fungi of tomato.fluorescent pseudomonas spp., isolated from rhizosphere soil of tomato and pepper plants, were evaluated in vitro as potential antagonists of fungal pathogens. strains were characterized using the api 20ne biochemical system, and tested against the causal agents of stem canker and leaf blight (alternaria alternata f. sp. lycopersici), southern blight (sclerotium rolfsii sacc.), and root rot (fusarium solani). to this end, dual culture antagonism assays were carried out on 25% tryptic soy agar, k ...201121507555
insecticidal activity and fungitoxicity of plant extracts and components of horseradish (armoracia rusticana) and garlic (allium sativum).to avoid environmental pollution and health problems caused by the use of traditional synthetic pesticides, there is a trend to search for naturally occurring toxicants from plants. among the compounds discussed for anti-fungal and insecticidal activity, the natural extracts from garlic and horseradish have attracted considerable attention. the objective of this study is to determine the insecticidal and anti-fungal activity of armoracia rusticana and allium sativum l. extracts against larvae of ...201121726146
synthesis, antifungal activity and structure-activity relationships of vanillin oxime-n-o-alkanoates.vanillin oxime-n-o-alkanoates were synthesized following reaction of vanillin with hydroxylamine hydrochloride, followed by reaction of the resultant oxime with acyl chlorides. the structures of the compounds were confirmed by ir, 1h, 13c nmr and mass spectral data. the test compounds were evaluated for their in vitro antifungal activity against three phytopathogenic fungi macrophomina phaseolina, rhizoctonia solani and sclerotium rolfsii by the poisoned food technique. the moderate antifungal a ...201223413571
scleroglucan compatibility with thickeners, alcohols and polyalcohols and downstream processing implications.thickening capacity and compatibility of scleroglucan with commercial thickeners (corn starch, gum arabic, carboxymethylcellulose, gelatin, xanthan and pectin), glycols (ethylene glycol and polyethylene glycol), alcohols (methanol, ethanol, 1-propanol and isopropanol) and polyalcohols (sorbitol, xylitol and mannitol) was explored. exopolysaccharides (epss) from sclerotium rolfsii atcc 201126 and a commercial scleroglucan were compared. compatibility and synergism were evaluated taking into accou ...201223399135
first report of sclerotium rot on cymbidium orchids caused by sclerotium rolfsii in korea.sclerotium rot was found on cymbidium orchids at seosan-si, chungcheongnam-do, korea, in july, 2010. symptoms occurred on low leaves, which turned yellowish, after which the entire plant wilted. severely infected plants were blighted and eventually died. white mycelial mats and sclerotia appeared on pseudobulbs. based on the mycological characteristics and pathogenicity, the causal fungus was identified as sclerotium rolfsii. this is the first report of new sclerotium rot on cymbidium spp. cause ...201223323053
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