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enhanced oncornavirus expression in marek's disease tumors from specific-pathogen-free chickens.an oncornavirus was recovered from cell cultures of kidney tumors from specific-pathogen-free chickens inoculated with marek's disease herpesvirus (mdhv). the mdhv inoculum was free of infectious avian leukosis virus (alv). direct examination of a variety of tissues from mdhv-inoculated chickens demonstrated increased levels of alv-specific rna compared to tissues from diluent-inoculated (control) chickens. dna from cultured kidney tumor cells annealed to an alv complementary dna probe at the sa ...1979216833
[serologic methods for the overall diagnosis of bovine herpes mamillitis].developed were methods of seroneutralization, agar gel precipitation, and complement-fixation test (cft) for the complex diagnosis of herpes mamillitis of herpes mamillitis in cattle. an agreement was found between the results obtained with the use of each method. highest sensitivity and specificity possessed the seroneutralization reaction with which a highest percent of positively reacting sera was revealed (18.6). however, the cft method could preferably be applied in mass investigations as i ...1978217144
proteins of equine herpesvirus type 3. i. polypeptides of the purified virion. 1979217165
treatment of experimental herpesvirus infections with phosphonoformate and some comparisons with phosphonoacetate.phosphonoformate (pf) at a concentration of 5 to 10 mug/ml inhibited the growth of type 1 strains of herpes simplex virus (hsv) in tissue culture, whereas 20 to 30 mug/ml was required for inhibition of type 2 strains and about 50 mug/ml was required for murine cytomegalovirus. in mice inoculated intraperitoneally or intracerebrally with hsv or intraperitoneally with murine cytomegalovirus, treatment with 250 to 400 mg of pf per kg twice daily for 5 days had only minimal effectiveness. when mice ...1978217300
effect of 9-(2-hydroxyethoxymethyl)guanine on herpesvirus-induced keratitis and iritis in rabbits.drugs used for the inhibition of dna viruses, such as iododeoxyuridine, adenine arabinoside, or trifluorothymidine, are not biochemically selective in their action and also interfere with normal cellular functions. the recently reported 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) is selectively phosphorylated by viral thymidine kinase but not by normal cellular thymidine kinase. our present studies show that the acycloguanosine is as effective in treating herpetic keratitis in the rabbit ...1978217301
mechanisms of recovery from herpesvirus infections -a review.a variety of specific immunological mechanisms have been shown to be effective at neutralizing herpesviruses or destroying herpesvirus infected cells. these include both humoral and cell mediated immune responses or combinations thereof. thus, it is genarlly accepted that humoral immunity is probably responsible for preventing reinfection whereas cellular immunity, mediated by t lymphocytes or by the interaction of antibody and fc receptor bearing cells, is more important in recovery from infect ...1978217506
a model for intramolecular recombination of herpesvirus dna leading to the formation of circular, circular-linear and fragmented genomes. 1978218060
[diagnosis of recurrent herpesvirus infection by means of viral precipitin studies]. 1977218265
[recurrent herpesvirus in the state of rio de janeiro]. 1977218266
maternal viral infection with adverse results: cytomegalovirus and herpesvirus. 1977218305
purification and characterization of deoxythymidine kinase (dtk) induced in dtk- 3t3 mouse cells by equine herpesvirus type 1 (ehv-1). 1979218350
biological and biochemical properties of defective interfering particles of equine herpesvirus type 1. 1979218357
extreme sensitivity of enveloped viruses, including herpes simplex, to long-chain unsaturated monoglycerides and alcohols.unsaturated monoglycerides and alcohols of chain lengths of 16 or 18 carbons were found to be extremely potent inactivators of two enveloped viruses, herpes simplex virus type 2 and bacteriophage phi6. the lipid-containing bacteriophage pm2 was also inactivated by some of these amphiphilic molecules. treatment of herpes simplex virus type 2 with these compounds at concentrations as low as 0.2 mum reduced virus survival to 50% in 30 min, making these agents the most potent inactivators of herpes ...1979218499
further study on the three-dimensional structure of the core of marek's disease virus and herpesvirus of turkey.three-dimensional structures of the core of marek's disease virus and herpesvirus of turkey were examined by the tilting apparatus of an electron microscope. various types of the core found in the infected cells were considered to represent developmental stages of the viruses. the basic structure of the core consisted of a toroid surrounding a cylindrical mass, which was clearly demonstrated by tilting the core in two directions. a cylindrical mass spooled by more than two toroids, which seemed ...1979218531
herpesvirus hominis antibodies among children and young adults in ibadan.a total of 422 sera collected from children and young adults living in ibadan, nigeria, has been examined for the presence of antibodies to type 1 and type 2 herpesvirus hominis. type 1 antibodies were rapidly acquired from the age of 1 year onwards, reflecting the relatively poor living standards and the overcrowded accommodation among the population studied. type 2 antibodies were acquired between the ages of 3 and 5 years. it is suggested that non-venereal spread of the virus must be responsi ...1979218671
epidemiology of equine upper respiratory tract disease on standardbred racetracks.the outbreaks of upper respiratory tract infections in horses at standardbred racetracks were investigated over a three year period. the most serious epidemics of respiratory disease occurred in the winter and spring seasons. both influenza viruses and equine herpesvirus 1 were shown to be present in the horse population. the herpesvirus was associated with respiratory disease particularly in the winter but the equine influenza viruses apparently were responsible for the major epidemics of respi ...1979218705
a comparative study of bovine herpesvirus 1247 and equine herpesvirus 1 in ponies.the clinical and immunological response of ponies exposed to a bovine herpesvirus isolate and equine herpesvirus 1 were compared. each virus was inoculated into two ponies by the intranasal route. one uninoculated pony was used with each group as a contact control. the four inoculated ponies developed a mild rhinitis with an increase in rectal temperature. virus was recovered from nasal secretions collected from the four inoculated and one contact pony. all ponies developed a serum neutralizing ...1979218710
antiherpesvirus activity in human sera and urines after administration of adenine arabinoside: in vitro and in vivo synergy of adenine arabinoside and arabinosylhypoxanthine in combination.the minimum inhibitory concentration (mic) of adenine arabinoside (ara-a) in rabbit kidney microtiter tissue cultures (rk-13) to a prototype strain of herpes simplex virus, type 1 (e115) based upon inhibition of cytopathic effects is 1.5 mug/ml. in this system, the mic of arabinosylhypoxanthine (ara-hx), the major in vivo metabolic derivative of ara-a, is 75 mug/ml. inhibition of cytopathic effects of herpes simplex virus, type 1 (hsv-1) in microtiter wells of rk-13 cells varies directly with th ...1978219024
characterization of an epstein-barr virus-induced dna polymerase.the addition of iododeoxyuridine to p3hr-i cell cultures led to a large increase in both epstein-barr virus (ebv)-induced dna polymerase activity and early antigen-positive cells. this ebv-induced dna polymerase was separated from the cellular alpha- and beta-polymerases by sequential column chromatography on sepharose 6b, deae-cellulose, and phosphocellulose, resulting in partial purification of about 320-fold. the partially purified-ebv dna polymerase could be distinguished from the cellular d ...1979219209
persistence of herpes simplex virus type 1 in rat neurotumor cells.herpes simplex virus type 1 (hsv-1) infection of a rat central nervous system tumor cell line led to almost complete destruction of the cells. cells that survived the infection could be isolated and shown to produce infectious hsv particles for variable lengths of time in culture ranging from 20 to 57 passages. even though infectious virus production eventually ceased, the cell lines continued to produce herpes-specified proteins as measured by immunological techniques. these cells also showed h ...1979219234
no homology detectable between marek's disease virus (mdv) dna and herpesvirus of the turkey (hvt) dna.the relation of four different strains of mdv and two strains of hvt was analyzed by gel electrophoresis of viral dna digested by various restriction endonucleases and by filter hybridization of viral dna with complementary rna. the four mdv strains showed fragment patterns completely different from those of hvt upon digestion of the viral dna with bam h i, eco r i, hind iii, hpa, i, and xho and separation of fragments on agarose gels. the cleavage patterns of the four mdv strains showed great s ...1979219323
respiratory viruses in hospital patients on the witwatersrand. a 7-year study.virological investigations were carried out on 4 151 patients with respiratory disease hospitalized between may 1966 and april 1972. the groups examined were black and white children and black miners. influenza viruses were more common among malnourished black children and tended to cause more severe disease. this was also true of adenovirus and herpesvirus hominis type 1 infections. adenoviruses appear to be secondary invaders, frequently after a measles or influenza attack. a generalized epide ...1979219551
[immunomorphological changes in chicks vaccinated against marek's disease with herpesvirus sz-1]. 1978219588
adenovirus-associated virus polypeptides synthesized in cells coinfected with either adenovirus or herpesvirus. 1979219599
minor dna homology between herpesvirus of turkey and marek's disease virus? 1979219602
persistence of herpes simplex virus types 1 and 2 in infected individuals.during a 23-month period, 18 patients with facial or genital herpetic lesions were examined; culture specimens from each patient were obtained three times per week for virologic studies. the isolated viruses were identified, and the average duration of herpesvirus in lesions was determined. herpes simplex virus type 1 (hsv-1) was isolated from facial lesions for a mean duration of 3 1/2 days. in contrast, herpes simplex virus type 2 (hsv-2) was isolated from genital lesions for a mean duration o ...1979219784
effectiveness of cell-free or cell-associated turkey herpesvirus vaccine against marek's disease in chickens as influenced by maternal antibody, vaccine dose, and time of exposure to marek's disease virus. 1978219826
in vitro inactivation of cell-free marek's disease herpesvirus by immune peripheral blood lymphocytes.the effect of peripheral blood lymphocytes (pbl) on cell-free marek's disease virus (mdv) was studied in vitro in pbl from intact or embryonal bursectomized (bx) chicks infected with the apathogenic sb-1 isolate of mdv. the pbl were harvested purified and were mixed, 200 or 2,000 to 1, with cell-free ga-5 of mdv. virus survival was assayed after incubation for 1 hour at 36 c by the standard assay for cell-free mdv. pbl from intact but not bx chickens were able to inactivate virus. when the effec ...1978219833
extraction of soluble antigens of epstein-barr virus, herpesvirus salmirl, and herpesvirus ateles with the use of glycine.for extraction of soluble antigen from cells infected with epstein-barr virus, herpesvirus salmirl, and h. ateles, 0.1 m glycine (ph 9.5) was used. this method yielded increased amounts of the antigen containing much less cell debris. lymphoblastoid cells infected with epstein-barr virus could maintain up to 50% viability after the extraction procedure. these cells could be used again after an appropriate interval in culture. the usefulness of this technique is discussed.1979220447
isolation of a cytomegalovirus from salivary glands of white-lipped marmosets (saguinus fuscicollis).minced salivary glands from seven white-lipped marmosets (saguinus fuscicollis and saguinus nigricollis) and one cotton-topped marmoset (saguinus oedipus) were cocultivated with marmoset cell cultures. a viral agent, designated ssg, was isolated from two saguinus fuscicollis. slowly progressing foci of rounded, vacuolated, refractile cells were first observed at 40-43 days incubation. electron microscopy revealed intranuclear herpesvirus nucleocapsids and intracytoplasmic and extracellular envel ...1979220464
experimental canine herpesvirus infection in newborn puppies, using a new zealand isolate. 1979220572
cesarean section and genital herpesvirus infection. 1979220577
replication of herpesvirus dna. iv: analysis of concatemers. 1979220789
episomal viral dna in herpesvirus saimiri-transformed lymphoid cell lines.structural analysis of episomal viral genomes from two herpesvirus saimiri (hvs)-transformed tumour cell lines (no. 1670 and 70n2) showed that both types of episomes have a higher molecular weight than linear virion dna. the arrangement of unique (l) and repetitive (h) dna in no. 1670 episomes was studied by partial denaturation mapping. part of the l-sequences present in linear virion dna was found to be missing, part was found to be duplicated in the episomes. the episomal l-dna regions were c ...1978221324
herpesvirus papio: state of viral dna in baboon lymphoblastoid cell lines.baboon lymphoblastoid cell lines (lcl) transformed with herpesvirus papio were evaluated for the state of hvp dna in the cells by caesium chloride density-gradient centrifugation and dna-crna filter hybridization. five hvp producer cultures and one non-producer culture were studied and non-integrated and integrated hvp dna were detected in all producer cultures whereas only integrated hvp dna could be demonstrated in the non-producer lcl. non-integrated hvp dna had a density of 1.715--1.716 g/cm ...1978221325
altered biological and biochemical properties of a phosphonoacetate-resistant mutant of herpesvirus of turkeys.a phosphonoacetate (pa)-resistant mutant of the herpesvirus of turkeys (hvt) was isolated and characterized. the mutant (hvtpa) replicates in growth medium containing 300 microgram/ml of pa and shows in vitro temperature sensitivity at 41 degrees c (its 37 degrees c/41 degrees c efficiency of replication is about 5). hvtpa replicates poorly in chickens and fails to provide complete protection against mdv challenge. the hvtpa-induced dna polymerase has an apparent inhibition constant for pa 10 ti ...1978221331
human herpesvirus 1 as a model of regulation of herpesvirus macromolecular metabolism: a review. 1978221332
identification and partial purification of two ebv-associated dna polymerases.virally induced dna polymerases have been demonstrated in cells infected with a variety of herpesviruses. these include herpes simplex virus (weissbach et al., 1973), marek's disease virus (boezi et al., 1974), equine herpesvirus (allen et al., 1977), and cytomegalovirus (huang, 1975a). recently a new iododeoxyuridine (iudr)-induced intracellular dna polymerase in an epstein-barr virus (ebv)-producing hybrid cell line has also been reported (miller et al., 1977). we present evidence that there a ...1978221345
herpesvirus production by ultraviolet-irradiated human skin cells: a marker of repair. 1978221346
oncogenic transformation of non-permissive murine cells by viable equine herpesvirus type 1 (ehv-1) and ehv-1 dna.primary cultures of balb/c mouse embryo fibroblasts infected with as much as 100 pfu per cell of ehv-1 do not exhibit cytopathology or synthesize detectable amounts of ehv-1 specific rna, dna, or infectious virus. addition of 1--2 microgram of non-fragmented ehv-1 dna as a co-precipitate with calcium phosphate to monolayers of such non-permissive mouse cells resulted in the appearance, after 4--6 weeks, of foci of piled-up, morphologically altered cells. cell lines established from such transfor ...1978221355
enhanced efficiency of transfection of hsv dna in hsv-1 dna-transformed hamster cells.the efficiency of transfection, i.e., the number of plaques per microgram of dna, was compared for herpes simplex virus (hsv) type 1 and type 2, and herpesvirus eidolon (antigenically unrelated to hsv) dnas in hsv dna-transformed cells (eh/a44) and control hamster cells (eht). the efficiency of transfection of hsv-1 and hsv-2 dnas was significantly higher in eh/a44 cells than in eht cells, showing that an early step in hsv infection was involved in the partial resistance of hsv dna-transformed c ...1978221357
characterization of the replicative structures of the dna of a herpesvirus (pseudorabies).the replication of prv dna occurs in two phases, early and late. during the early stages of infection newly synthesized dna is associated with molecules sedimenting with an s-value up to two-fold greater than that of mature viral dna. these molecules represent unit-size linear or circular molecules, as well as small concatemers in the process of replication. initiation of replication occurs at a site situated 20 micron from one of the ends as well as at or near the end of the molecules. at later ...1978221363
arabinosylthymine: in vivo effectiveness in a systemic herpesvirus infection. 1978221367
potential for immunization against herpesvirus infections with plasma membrane vesicles.virus-free, membrane antigen (ma)-positive plasma membrane vesicles produced from herpesvirus saimiri-infected owl monkey cells were used as immunogens in cotton-top marmosets. neutralizing antibodies as well as antibodies to ma and hvs-induced la were induced following immunization of marmosets with these vesicles. preliminary findings demonstrated that immunized marmosets were significantly less susceptible to tumour induction by hvs than control animals.1978221369
provisional classification of herpesviruses: herpesvirus study group of the international committee for the nomenclature of viruses. 1978221375
achievements, priorities and needs in the lucké tumour herpesvirus field. 1978221378
oncogenesis and herpesviruses iii. part 2: cell-virus interactions, host response to herpesvirus infection and associated tumours, role of co-factors. 1978221380
pronephric tumour cell lines from herpesvirus-transformed cells.pronephric tumor cell lines have been established from tumours induced after inoculation of embryos with herpesvirus cultivated in vitro. neoplastic properties of the lines are characterized.1978221384
the immunology of primary and recurrent herpesvirus infection: an overview. 1978221390
persistent, chronic, and latent infections by herpesvirus: a review. 1978221391
comparison of humoral and cell-mediated responses and virus markers in herpesvirus-associated lymphomas: a review. 1978221406
prospects for control of herpesvirus-related diseases: a review. 1978221415
vaccination against diseases associated with herpesvirus infections in animals: a review.an account is presented of the development and use of herpesvirus vaccines in domestic animals, with particular reference to those viruses causing cytolytic rather than oncogenic infections. the chief infections covered are aujeszky's disease (ad or pseudorabies), infectious bovine rhinotracheitis (ibr) and equine rhinopneumonitis (equine abortion; ehv-1). others mentioned are feline viral rhinotracheitis and malignant catarrhal fever of cattle. both live-modified and inactivated vaccines are wi ...1978221416
the interaction between herpesvirus and oncornavirus of guineapigs: in vitro and in vivo studies.in vitro, cultured guineapig doubly infected with both guineapig herpesvirus (gphlv) and guineapig oncornavirus (gpov) revealed pseudotype virus particles with oncornavirus morphology coated with gphlv antigen as detected by immunoferritin electron microscopy. in vivo, infection of hartley guineapigs with both gphlv and gpov induced hyperplasia in lymph-nodes in a higher percentage of animals than in those that received only one virus type. in addition, experimental gphlv infection of strain 2 g ...1978221417
effect of ara-t on the replication of herpes simplex virus, varicella-zoster virus and cytomegalovirus.the thymidine analogue, 1-beta-arabinofuranosylthymine (ara-t), has previously been found to selectively inhibit herpes simplex virus (hsv) replication. at a relatively non-toxic concentration (50 microgram/ml), ara-t reduced hsv yields by a factor of 10,000-100,000. ara-t was also effective in inhibiting the replication of variecella-zoster virus (vzv) in vitro in human embryo fibroblasts, completely preventing vzv-specific cytopathic effects (cpe). ara-t reduced the cell-free virus and plaque- ...1978221418
successful treatment of human genital herpes infections with 2-deoxy-d-glucose.thirty-six women with genital herpes infections (proved by virological or cytological means) were treated in a double-blind placebo-controlled study with the glucose analogue 2-deoxy-d-glucose for a three-week period. in initial mucocutaneous cases, 89% were cured, with two recurrences after 24 months; in the case of recurrent or secondary infections, 90% had a notable improvement manifested by no or less-frequent recurrences, fewer lesions, or shortened duration of symptoms. in initial infectio ...1979221691
route of herpesvirus spread traced with aid of dna-cleaving enzymes. 1979221697
lymphoid neoplasms in chicken flocks free of infection with exogenous avian tumor viruses.more than 4,500 breeding female chickens of nine inbred lines maintained under specific-pathogen-free conditions to approximately 500 days of age were studied. routine monitoring and special assays indicated that they were free of infection by exogenous viruses of the leukosis-sarcoma and the reticuloendotheliosis groups. some birds were maintained free of marek's disease (md) virus infection in plastic isolators, and others were maintained in conventional chicken houses and vaccinated with the ...1979221715
prevention of reactivated herpes simplex infection by human leukocyte interferon after operation on the trigeminal root.microneurosurgical procedures on the trigeminal-nerve root are often followed by reactivation of herpes simplex virus infection, manifested by herpes labialis or oropharyngeal herpesvirus shedding or both. in a double-blind study of the ability of human leukocyte interferon to prevent this reactivation, patients with a history of herpes labialis were given 7 x 10(4) u of interferon per kilogram of body weight per day or placebo for five days beginning on the day before operation. in 18 patients ...1979221812
detection of a nuclear antigen in herpesvirus ateles-carrying marmoset lines by the acid-fixed nuclear binding technique.in vitro binding of a herpesvirus ateles (hva)-associated soluble antigen to amphibian erythrocyte nuclei was demonstrated by the acid-fixed nuclear binding technique in combination with anticomplement immunofluorescence. incubation of concentrated salt-extracted soluble antigens derived from hva-carrying marmoset lines with methanol/acetic acid-fixed erythrocytes of frogs and salamanders resulted in a brilliant nuclear fluorescence after exposure to a live virus-boostered, anti-hvs-positive squ ...1979221913
sensitivity and specificity of diagnostic tests for genital infection with herpesvirus hominis.three smear techniques for diagnosis of herpesvirus hominis infection were evaluated by study of 168 unselected patients with genital lesions attending a venereal disease clinic. a tissue culture and results of three smear techniques were obtained for each patient in random order. h. hominis was recovered from 101 patients (60%). all three smear tests (immunofluorescence, papanicolaou, and crystal violet) were highly specific, but the sensitivity of each was smaller than or equal to 40% among pa ...1979221993
risk factors in lip cancer: a questionnaire survey.an analysis was made of potential risk factors of cancer of the lip in finland. the series comprised 290 lip cancer patients, and the controls were 254 patients with squamous cell carcinoma of the skin of the head and neck, all reported to the finnish cancer registry in 1972-1973. the results obtained in a questionnaire survey (response rate, 75%; 54% of the total series) indicated that the male lip cancer patients had engaged in outdoor work and smoking with significantly greater frequency than ...1979222137
immune control of herpesvirus latency. 1979222416
bovine abortion associated with mixed movar 33/63 type herpesvirus and bovine viral diarrhea virus infection.a herpesvirus and cytopathogenic bovine viral diarrhea virus were isolated from a bovine fetus aborted in the 6th month of gestation. the herpesvirus was serologically indistinguishable from bovine herpesviruses dn-599 and movar 33/63.1979222535
persistent infection of a human lymphoblastoid cell line with equine herpesvirus 1.infection of a human lymphoblastoid cell line (jijoye line derived from a burkitt lymphoma which contains epstein-barr virus) with equine herpesvirus 1, maintained and observed for 53 days, was characterized by the continuous production of infectious extracellular and intracellular virus. maximum virus production correlated with active cell multiplication. less than 15% of the cells possessed viral capsid antigen at any one time. five percent of the cells in the jijoye line possess epstein-barr ...1979222688
pleocytosis and immunoglobulin changes in cerebrospinal fluid and herpesvirus serology in patients with guillain-barré syndrome.in a follow-up study of 24 patients with guillain-barré syndrome, 55% developed a cerebrospinal fluid (csf) mononuclear pleocytosis, which persisted for 4 months or more. raised index values of csf-immunoglobulin g (igg), csf-iga, and csf-igm, indicating synthesis of the immunoglobulin in question in the central nervous system, were found in 63, 35, and 25%, respectively. agarose gel eletrophoresis revealed oligoclonal immunoglobulin in csf in 21% and in both csf and serum in another 21% of the ...1979222797
comparison of indirect hemagglutination and indirect immunofluorescence tests with microneutralization tests for detection of type-specific herpesvirus hominis antibody.indirect hemagglutinating and immunofluorescent antibody responses to herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom h. hominis type 1 or 2 was isolated. the indirect immunofluorescent antibody test was shown to be the most sensitive and specific for primary human infections. the sensitivity and specificity of the indirect hemagglutination and the immunofluorescent antibody tests were shown to be equal to that of the microneutraliz ...1979222800
simultaneous radioimmunoassay for specific antibodies to members of the human herpesvirus group.a method of described for the simultaneous radioimmunoassay (ria) for antibody to members of the human herpesvirus group. the ria is compared with some of the conventional serologic techniques used to quantitate antibody to these viruses (epstein-barr virus, cytomegalovirus, herpesvirus type 1 and varicella-zoster virus). color-coded beads, each coated with the antigens of a different herpesvirus, were similtaneously placed in a well which contained a human serum to be assayed for antibody to ea ...1979222846
isolation of a herpesvirus from an american kestrel with inclusion body disease.a herpesvirus was isolated from the liver of a captive-bred american kestrel (falco sparverius) which had died of inclusion body disease. initial isolation was achieved in chicken embryo fibroblasts after three blind passages. cell-adapted virus produced a distinct rounding of cef cells within 24 to 48 h. biologic and serologic tests suggested that the kestrel virus is similar to falcon herpesvirus and pigeon herpesvirus and is at least partially related to owl herpesvirus. however, serologic te ...1979222917
expression of genetic resistance to an oncogenic herpesvirus at the target cell level. 1979223067
persistence of virus shedding in asymptomatic women after recovery from herpes genitalis.one hundred seventeen women were reexamined within 6 months after presenting with genital herpes. swabs for virus isolation were taken from the cervix whether or not lesions were observed. herpesvirus type ii was isolated from 30 of 67 patients with recurrent disease, but also from 5 of 50 women during periods when there were no signs or symptoms of illness. the epidemiologic importance of virus shedding from asymptomatic women is discussed.1979223095
some aspects of the role of viruses in cancer.the cells of tumours induced by many oncogenic dna viruses, or cells transformed in vitro, contain virus-specific t and transplantation antigens; these have been described for sv40 virus, polyoma virus and adenoviruses. the investigation of viruses as causes of malignant disease in man has sought to establish whether tumour cells possess these virus-specific proteins; however, to date and with the limitations of present techniques, this enquiry has not demonstrated the above viruses as causal of ...1979223141
sites of sequence variability in epstein-barr virus dna from different sources.the intracellular epstein-barr virus (ebv) dna present in virus-transformed cells was partly purified from 23 cell lines or biopsies of burkitt lymphoma, nasopharyngeal carcinoma, infectious mononucleosis, or healthy carrier origin. such dna was cleaved in fragments (a-k) of molecular weights between 1 x 10(6) and 30 x 10(6) with restriction enzyme ecori, and these fragments were analyzed by standard methods involving agarose gel electrophoresis, transfer to nitrocellulose filters, and hybridiza ...1979223159
prior immunization by herpesvirus. 1979223444
differentiation of field strains and a vaccine strain of equine herpesvirus 1, using plaque characteristics. 1979223480
bovine herpesvirus 1--infection of the upper alimentary tract of cattle and its association with a severe mortality. 1978223538
infection studies on a reticuloendotheliosis virus contaminant of a commercial marek's disease vaccine.reticuloendotheliosis virus (rev) was isolated in cell cultures from commercial marek's disease (herpesvirus of turkeys) vaccine and re-isolated from the organs of vaccinated chickens. runting and feathering abnormalities were produced when 1-day-old specific pathogen free chickens were inoculated with rev. histopathological lesions in infected chickens were hypoplasia of the thymus, bursa and spleen, and inflammation of the proventriculus, kidneys and liver. serological responses to rev were de ...1979223539
changes in the lymphoid organs of mice after a short treatment with diphenylhydantoin and inoculation with an herpesvirus subtype. 1978223608
serodiagnosis of herpesvirus infection in primates.the presence of herpesviruses in various animal tissues used for vaccine production is of importance, inasmuch as these viruses may go unrecognized, residing in latent form. of greatest concern is herpesvirus simiae (b virus) present in the majority of macaca species, one of the more extensively used laboratory nonhuman primates. the close antigenic relationship of b virus to human herpes strains (h. hominis) and sa8 (a baboon herpesvirus) makes serologic differentiation extremely difficult, but ...1978223911
genital chlamydia trachomatis infections in patients with cervical atypia.of 177 gynecologic outpatients with cervical dysplasia studied, 29 (16%) were found to excrete chlamydia trachomatis from their urogenital tract. sera collected from 93 of these patients were significantly more often positive for and showed higher levels of antichlamydial micro-complement fixation (cf) and immunofluorescence (if) antibodies than sera obtained from the controls. however, when the sera were tested for anti-herpesvirus type ii (hsv-ii) and cytomegalovirus (cmv) antibodies, no diffe ...1979224353
transcription of the genome of pseudorabies virus (a herpesvirus) is strictly controlled. 1979224585
[isolation and identification of the infectious bovine rhinotracheitis virus in a bovine fetus kidney (ibr/ipv)].the authors describe the isolation and identification of a virus from bovine fetal kidney cells maintained in culture for 30 to 45 days. the virus was characterized as a herpesvirus of the infectious bovine rhinotracheitis in cell culture by the evidence of cytopathogenic effect in bovine kidney and testis cultures and serologically by a neutralization test using rabbit anti-infectious bovine rhinotracheitis sera.1978224840
lymphocyte transformation for the detection of herpesvirus-induced tumor-associated antigens.cell-mediated immunity to fibroblasts transformed by herpes simplex virus type 2 was investigated with a lymphocyte assay system. the assay system was first standardized with phytohemagglutinin, a nonspecific stimulator of blastogenesis. hamster splenic and blood lymphocytes reacted to phytohemagglutinin with a dose-response curve similar to that reported for other rodent species. splenic lymphocytes from hamsters bearing isografts, induced by herpes simplex virus type 2, were transformed by cel ...1979225013
defective reactivation of ultraviolet light-irradiated herpesvirus by a bloom's syndrome fibroblast strain.we have used the technique of host cell reactivation of uv-irradiated herpes simplex virus type 1 as a measure of the repair capacity of three bloom's syndrome skin fibroblast strains. at low multiplicity of infection (less than 6 x 10(-4) plaque-forming unit/cell), reactivation of the virus by the bloom's syndrome strains was indistinguishable from that by normal strains. reactivation at higher multiplicities was measured using an infectious centers assay. at 3 plaque-forming units/cell, surviv ...1979225021
induction of virus-neutralizing antibody by glycoproteins isolated from chicken cells infected with a herpesvirus of turkeys.the production of virus-induced proteins in chicken embryo fibroblast cells infected with a herpesvirus of turkeys was studied. it was found that glycoproteins isolated from membrane-rich fractions of infected cells by affinity chromatography using concanavalin a induced neutralizing and precipitating antibody in rabbits and chickens. after analytical electrophoresis, such isolates were found to contain three polypeptide bands of between 100 x 10(3) and 120 x 10(3) molecular weight not present i ...1979225277
membrane glycoproteins and antigens induced by human cytomegalovirus.early after infection of cells with cytomegalovirus, the membranes were modified with respect to both glycoprotein composition and immunological specificity. virus-specified antigens were inserted into the plasma membrane at 24 h after infection, as much as 2 days before virion and dense body maturation. although at least four virus-induced glycoproteins were synthesized and bound to plasma and microsomal membranes between 20 and 24 h after infection, virus-specified antigen accumulated primaril ...1979225413
phosphorylation of arabinofuranosylthymine in non-infected and herpesvirus (tk+ and tk-)-infected cells.the phosphorylation of arabinofuranosylthymine (arathd) has been studied both in non-infected cells and in those infected with herpes simplex virus (hsv-1, lennette; hsv-1, ies and hsv-2, d-316). in these experiments, hsv strains were used which either contain (lennette, tk+ and d-316 tk+) or lack (ies, tk-) the capacity to induce pyrimidine deoxyribonucleoside kinase. it was found that extracellularly administered arathd is phosphorylated to ara ttp via aratmp and aratdp in both non-infected an ...1979225422
immunity to equine herpesvirus 1 infection in foals during the first year of life.a band of 23 pregnant mares on a thoroughbred breeding farm all had serum virus-neutralizing antibody titres to equine herpesvirus 1 (ehv-1). antibody was not transferred to their foals in utero. all foals received antibody from colostrum and developed antibody titres similar to their dams. the serum virus-neutralizing antibody titres were observed in 10 of these foals for 1 year. decay of passive immunity occurred at the rate of 3.25 two-fold dilutions in 100 days and reached zero at the mean t ...1979225497
squirrel monkey retrovirus and herpesvirus saimiri: observation in the same cell following isolation.electron microscopic examination of mink lung cells previously cultured with a squirrel monkey (saimiri sciureus) throat swab suspension revealed the presence of squirrel monkey retrovirus (smrv) and herpesvirus saimiri (hvs) coexisting within the same cells in culture. hvs was identified by serum neutralization, and the retrovirus isolate was identified as smrv by a morphologic examination, microimmunodiffusion analysis, and demonstration of an mg2+ preference for the rna-directed dna polymeras ...1979225504
regulation of herpesvirus macromolecular synthesis. viii. the transcription program consists of three phases during which both extent of transcription and accumulation of rna in the cytoplasm are regulated.this report concerns the stable viral rna sequences that accumulate in hep-2 cells infected with herpes simplex virus type 1. by hybridizing labeled total dna and restriction endonuclease dna fragments with excess unlabeled total nuclear and cytoplasmic rna, we determined the genetic complexity of the rna and we mapped the regions on the physical map of herpes simplex virus type 1 dna that are homologous to the rna. our results show the following. (i) the viral rnas accumulating in the nucleus a ...1979225555
antigenic comparisons between herpesviruses isolated from fallow deer in alberta and the viruses of infectious bovine rhinotracheitis, equine rhinopneumonitis and dn-599, a non-ibr bovine herpesvirus.antigenic comparison studies of three herpesviruses isolated from fallow deer (dama dama) in alberta and herpesviruses from some domestic species were carried out by the alpha serum-virus neutralization test. complete cross neutralization was demonstrated among the deer herpesviruses and equine herpesvirus type 1.1979225575
proteins of herpesvirus type 2. v. isolation and immunologic characterization of two viral proteins in a virus-specific antigenic fraction. 1979225865
[experimental myocardiopathy caused by the herpesvirus].mice weighing 18--20 g were inoculated with herpes simplex virus type i. the rate of pathological changes in the myocardium was found to depend on the route of virus inoculation and the time of heart examinations in the infected animals. the development of myocardiopathies was not determined by the virus dose used in the test. an immunosuppressant drug, imurane, reduced the rate of heart affections. pathomorphological changes in the heart were of parenchymatous-interstitial nature, with the lead ...1979225882
latent infection of cattle with bovid herpesvirus 2.in a series of experiments, 10 calves were infected by different routes with bovine mammillitis strain of bovid herpesvirus 2 (bhv2). nine of the calves developed lesions and bhv2 was isolated from 8 calves. at intervals, after clinical recovery, the calves were given a course of corticosteroid by intravenous injection. subsequently 7 calves developed lesions and from 3 calves bhv2 was reisolated. the shortest interval between initial infection and reisolation of virus was 10 weeks. in one calf ...1979226033
possible pitfalls in the study of igg receptors produced by herpesvirus-infected cells. brief report.the presence of specific antibodies to human herpesviruses in certain purified human igg preparations from commercial sources may produce misleading results when such preparations are used in studied on the igg receptors produced by virus-infected cells.1979226037
effects of avian reovirus on marek's disease (md). i. suppression of md development.the effects of an avian reovirus on the development of acute marek's disease (md) were studied by exposing chickens to reo-w isolant of avian reovirus before infecting them with an oncogenic md herpesvirus (mdhv). four separate trials were made in a similar manner, 2 trials with an experimental line of white leghorn (wsu-vs) highly susceptible to md, and 2 trials with a commercial strain of meat-type (c-mt) chicken. md mortality and/or incidence of gross md lesions were consistently reduced by p ...1979226049
[severe respiratory disease caused by herpesvirus in amazon chickens]. 1979226061
immune response of cattle to antigens obtained from bovine herpesvirus 1-infected tissue culture.the ability of two antigens, termed ev and cm, derived from bovine herpesvirus 1 infected cultures to produce serum-virus neutralizing antibodies has been studied in cattle. both ev and cm when inoculated with adjuvant induced significant increases in serum-virus neutralizing antibody titers. control groups inoculated in a similar manner failed to induce significant increases in serum-virus neutralizing antibody. some of the animals were vaccinated, then were bred, challenged with a virulent str ...1979226245
equine herpesvirus 1 infection in mares vaccinated with a live-virus rhinopneumonitis vaccine attenuated in cell culture.vaccination, in july and again in either november or december 1976, of 55 pregnant standardbred mares with a live-virus rhinopneumonitis vaccine attenuated in cell culture failed to protect some mares from infection with equine herpesvirus 1. from 1976-12-08 to 1977-03-08, 33 mares foaled healthy foals, 16 mares foaled dead foals or live foals which died usually within 48 hours and six mares aborted. gross and histological examinations and virus isolation studies confirmed that equine herpesviru ...1979226261
lymphotropic papovaviruses isolated from african green monkey and human cells.a lymphotropic papovavirus was isolated from a lymphoblastoid cell line of african green monkey (agm) cells which also contained a herpesvirus and a paramyxovirus-like agent. the papovavirus was analyzed by restriction endonuclease cleavage; its biochemical and serological crossreactivity with sv40 and host range have been determined. thus far, only b-lymphoblasts of primate and human origin have been found to be susceptible to infection. although more than 50% of the tested monkey sera were rea ...1979226854
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