induction of morphogenesis in the genus arthrobacter.thirty-eight different compounds were used with 17 species of arthrobacter to determine their ability to support growth and to induce the morphogenic cycle. in most cases, when a compound supported growth, it also induced the rod phase of growth. however, in a few cases, a compound would support growth with cells remaining in the coccoidal phase throughout the growth cycle. arthrobacter crystallopoietes was unique in the most compounds that supported growth did not induce the rod phase of the mo ...19751184572
fine structure of arthrobacter crystallopoietes during long-term starvation of rod and spherical stage cells. 19734119517
gram characteristics and wall ultrastructure of arthrobacter crystallopoietes during coccus-rod morphogenesis.arthrobacter crystallopoietes growing exponentially as cocci were changed to rods by adding succinate to the medium. cells were sampled before, during, and after this transition for gram-staining and ultrastructural studies. cells were gram stained by the standardized method of bartholomew, and all samples were fixed and prepared for thin sectioning in an identical manner. cocci were gram positive, and thin sections demonstrated a gram-positive type of cell wall having an average thickness of 31 ...19734121451
selective inhibition of bacterial enzymes by free fatty acids.octanoic acid inhibits, in vitro, the bacterial enzymes glucose-6-phosphate dehydrogenase, phosphofructokinase, pyruvate kinase, fumarase, lactate dehydrogenase, and the malic enzyme of arthrobacter crystallopoietes. the free fatty acid appears to act as an inhibitor of lipogenesis, although it does not affect the rate of gluconeogenesis. to demonstrate that this inhibition may be of physiological significance in vivo, those enzymes not involved in lipogenesis, such as fructose-1, 6-diphosphatas ...19694306971
enzyme induction and repression in arthrobacter crystallopoietes. 19724347452
lipid composition of growing and starving cells of arthrobacter crystallopoietes.the lipid composition of growing and starving cells of arthrobacter crystallopoietes was compared. although the lipid composition of the two cell types was similar, the amount of total lipids recovered from the starving cells was 30.4% less than that recovered from the growing cells. the loss of lipids, as compared to the loss of total cell mass during starvation, was (i) proportional to the loss of the cell mass (phosphatidylinositol, phosphatidylglycerol-2, and cardiolipin), (ii) greater than ...19724669211
changes in rna transcription during morphogenesis of arthrobacter crystallopoietes. 19734723945
biosynthesis of the peptidoglycan of bacterial cell walls. 8. specificity in the utilization of l-alanyl transfer ribonucleic acid for interpeptide bridge synthesis in arthrobacter crystallopoietes. 19685638593
activity of an autolytic n-acetylmuramidase during sphere-rod morphogenesis in arthrobacter crystallopoietes. 19685732515
long-term starvation survival of rod and spherical cells of arthrobacter crystallopoietes.spherical and rod-shaped cells of arthrobacter crystallopoietes, harvested during exponential growth, were subjected to total starvation for periods of time as long as 80 days. viability measurements were made by plate count and slide culture procedures. both cell forms remained 100% viable for 30 days. thereafter, viability of rods and spheres decreased equally at a slow rate. after 60 days of starvation, more than 65% of both cell forms were viable. no significant cell lysis occurred as eviden ...19704919984
reclassification of micrococcus agilis (ali-cohen 1889) to the genus arthrobacter as arthrobacter agilis comb. nov. and emendation of the genus arthrobacter.phylogenetic evidence derived from a 16s ribosomal dna analysis indicated that the type strain of micrococcus agilis, dsm 20550 (= atcc 966 = ccm 2390), is less closely related to the type species of the genus micrococcus, micrococcus luteus, than to the type species of the genus arthrobacter, arthrobacter globiformis, and related arthrobacter species. the phylogenetic position of m. agilis is supported by the presence of peptidoglycan variation a3 alpha and by the presence of mk-9(h2) as the ma ...19957547308
survival of arthrobacter crystallopoietes during prolonged periods of extreme desiccation.cells of arthrobacter crystallopoietes mixed in sand and air-dried have survived for up to 6 months after an initial period in which approximately half the cells lost their viability. comparative survival curves have been obtained from inoculated sands maintained under caso(4) or p(2)o(5). selections for more desiccation-resistant progeny capable of surviving the initial period were unsuccessful. both the coccoid and rod-shaped forms are equally resistant to several months of desiccation. desicc ...19734345921
lipid composition of some species of arthrobacter.the lipids from arthrobacter crystallopoietes, a. pascens, and a. globiformis were investigated. each strain contained three glycolipids, a monogalactosyl diglyceride, a digalactosyl diglyceride, and a dimannosyl diglyceride, and traces of triand tetraglycosyl diglycerides. the phospholipids in all three strains consisted of bisphosphatidylglycerol, phosphatidylglycerol, and phosphatidylmyoinositol. no evidence could be obtained for the occurrence of mannophosphoinositides. analysis of the fatty ...19714327507
a crystalline pigment produced from 2-hydroxypyridine by arthrobacter crystallopoietes n.sp. 196314106078
the phosphofructokinase of arthrobacter crystallopoietes. 19694247776
regulation of cyclic amp levels in arthrobacter crystallopoietes and a morphogenetic mutant.the extracellular levels of cyclic amp (camp), camp phosphodiesterase activity, and adenylate cyclase activity were measured at various intervals during growth and morphogenesis of arthrobacter crystallopoietes. there was a significant rise in the extracellular camp level at the onset of stationary phase, and this rise coincided with a decrease in intracellular camp. the phosphodiesterase activity measured in vitro increased in the early exponential phase of growth as intracellular camp decrease ...1978207674
stability of enzymes in starving arthrobacter crystallopoietes.cell-free extracts prepared from spherical and rod-shaped cells of arthrobacter crystallopoietes were assayed for enzymes during various periods of starvation. the level of nadh oxidase dropped to 20 and 30%, respectively, in spherical and rod-shaped cells during the first 1 to 2 days of starvation and then remained constant for 9 days. catalase activity decreased continuously and reached a low level in 9 days. enzymes involved in glucose metabolism and the tricarboxylic acid cycle were stable f ...1976180237
[bacterial population dynamics in a soil--plant system].the dynamics of rhizobium leguminosarum and arthrobacter crystallopoietes populations introduced into soil at different levels of density was studied in a zone near barley roots. microbial life with a high rate of growth was found only at the root surface. for a. crystallopoietes, the ultimate bacterial incidence at the root surface was found to depend on the original level of population density. for rh. leguminosarum, the ultimate incidence was shown to reach an identical level irrespective of ...19817219211
sphere-rod morphogenesis in arthrobacter crystallopoietes. ii. peptides of the cell wall peptidoglycan.cell walls of arthrobacter crystallopoietes grown as spheres and as rods were solubilized by treatment with the b enzyme from chalaropsis, an n-acetylmuramidase. the neutral glycopeptides were then isolated by chromatography on ecteola cellulose. the glycopeptides, consisting of disaccharide-peptide units interlinked by peptide cross-bridges, were fractionated by gel filtration on sephadex columns into oligomers of various sizes. the size distribution ranged from monomers with no cross-bridges t ...19676035267
sphere-rod morphogenesis in arthrobacter crystallopoietes. i. cell wall composition and polysaccharides of the peptidoglycan.cell walls of arthrobacter crystallopoietes were prepared from cells grown as spheres and from peptone- and succinate-induced rod stage cells. undegraded polysaccharide backbones of the peptidoglycans were isolated from myxobacter al-1 protease digests by ecteola cellulose and sephadex g-50 chromatography. the polysaccharide backbones of the sphere cell wall peptidoglycan are heterogeneous in their size, and average less than 40 hexosamines per chain. those of the rod cell walls are homogeneous ...19676035266
ultrastructural explanation for snapping postfission movements in arthrobacter crystallopoietes.the ultrastructure of dividing rod-stage cells of arthrobacter crystallopoietes was examined by electron microscopy. the cell walls consist of two layers. during cell division, the inner layer invaginates to form the septum. the outer layer does not participate in septum formation. after septum formation is completed, the two daughter cells remain attached by the outer layer of the cell wall. it appears that localized rupture of the outer layer during further wall growth is responsible for the p ...19715541021
flow-through chemiluminescence sensor using immobilized histamine oxidase from arthrobacter crystallopoietes kait-b-007 and peroxidase for selective determination of histamine.a flow sensor with immobilized oxidases is proposed for the determination of histamine in fish meat. chemiluminometric measurement of histamine was based on the luminol reaction with hydrogen peroxide produced by immobilized histamine oxidase (ec 1.4.3.-.) and peroxidase (ec within a flow cell. histamine oxidase was found in cells of arthrobacter crystallopoietes kait-b-007 isolated from soil. the oxidase and peroxidase were coimmobilized covalently on tresylated hydrophilic vinyl pol ...200111990588
nutritional control of morphogenesis in arthrobacter crystallopietes.ensign, jerald c. (university of illinois, urbana), and r. s. wolfe. nutritional control of morphogenesis in arthrobacter crystallopoietes. j. bacteriol. 87:924-932. 1964.-arthrobacter crystallopoietes exhibits the cyclic, morphological variation which is a characteristic of this genus. a simple chemically defined medium was developed in which this organism is restricted to growth and division entirely in the coccoid form. addition singly to this medium of l-arginine, l-phenylalanine, l-asparagi ...196414137632
[phylogenetic identification and differentiation of arthrobacter crystallopoietes subtypes utilizing maldi-ms]. 201223610926
lysis of bacterial cell walls by an enzyme isolated from a myxobacter.ensign, j. c. (university of illinois, urbana), and r. s. wolfe. lysis of bacterial cell walls by an enzyme isolated from a myxobacter. j. bacteriol. 90:395-402. 1965.-an exoenzyme which lyses intact cells, heat-killed cells, and cell walls of arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain al-1). the lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yea ...196514330733
immersion refractometry of isolated bacterial cell walls.immersion-refractometric and light-scattering measurements were adapted to determinations of average refractive indices and physical compactness of isolated bacterial cell walls. the structures were immersed in solutions containing various concentrations of polymer molecules that cannot penetrate into wall pores, and then an estimate was made of the polymer concentration or the refractive index of the polymer solution in which light scattering was reduced to zero. because each wall preparation w ...19734201772
metabolism of endogenous trehalose by streptomyces griseus spores and by spores or cells of other actinomycetes.the disaccharide trehalose is accumulated as a storage product by spores of streptomyces griseus. nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. in contrast, spores rapidly depleted their trehalose pools during the first hours of germination. extracts of dormant spores contained a high specific activity of the enzyme trehalase. the level of trehalase remained relatively constant during germination or incubation in buffer. nongerminating sp ...19873117770
[plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of arthrobacter].arthrobacter crysallopoietes strain km-4 degrading 2,6-dimethylpyridine and strain km-4a degrading both 2,6-dimethylpyridine and pyridine, arthrobacter sp. km-4b degrading 2,4-dimethylpyridine were isolated from soil. arthrobacter crystallopoietes km-4 and arthrobacter sp. km-4b contain 100 md plasmids pbs320 and pbs323. arthrobacter crystallopietes km-4a harbours a 100 md and 80 md plasmids. plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation ...19921454076
macromolecular synthesis and cell division during morphogenesis of arthrobacter crystallopoietes.the sphere-rod-sphere morphology cycle of arthrobacter crystallopoietes was accompanied by changes in the rate of growth and the rates of dna, rna and protein synthesis. the patterns of macromolecule synthesis resembled those found in other bacteria during a step-up followed by a step-down in growth rate. during the step-up in growth spherical cells grew into rods and macromolecules were synthesized in the absence of cell division. during step-down, successive rounds of septation produced progre ...19761015960
growth and pigment production by arthrobacter pyridinolis n. sp.a new bacterium capable of growing on 2-hydroxypyridine as sole source of carbon and nitrogen was isolated from soil. during its growth on solid medium, approximately 50% of this substrate was converted to a brilliant blue crystalline pigment which was deposited extracellularly in the colony mass. the pigment was identical to that produced by arthrobacter crystallopoietes during its growth on 2-hydroxypyridine. the new isolate exhibited the typical cycle of morphogenesis characteristic of the ge ...19761015948
isolation and characterization of morphogenetic mutants of arthrobacter crystallopoietes.mutants of arthrobacter crystallopoietes that exhibited altered ability to undergo the normal sphere-to-rod-to-sphere morphogenetic cycle were isolated. the procedure used to isolate these mutants involved velocity sedimentation in a sterile sucrose gradient to separate morphogenesis-deficient spherical cells from rod-shaped cells capable of normal morphogenesis. three classes of mutants were obtained: (i) those that cannot form rods, (ii) those that cannot form long rods, and (iii) those that f ...1978681281
adenylate nucleotide levels and energy charge in arthrobacter crystallopoietes during growth and starvation.the adenylate nucleotide concentrations, based on internal water space, were determined in cells of arthrobacter crystallopoietes during growth and starvation and the energy charge of the cells was calculated. the energy charge of spherical cells rose during the first 10 h of growth, then remained nearly constant for as long as 20 h into the stationary phase. the energy charge of rod-shaped cells rose during the first 4 h of growth, then remained constant during subsequent growth and decreased i ...1979518238
adenosine triphosphate pool levels and endogenous metabolism in arthrobacter crystallopoietes during growth and starvation.the adenosine triphosphate (atp) content of arthrobacter crystallopoietes was measured during growth, starvation and recovery from starvation. during exponential growth of the cells as spheres in a glucose slats medium, the level of atp per cell remained constant at 8.0 x 10(-10) micrograms/cell. morphogenesis to rodshaped cells and an increased growth rate following addition of casein hydrolysate was accompanied by an almost two-fold increase in the atp level. as division of the rod-shaped cell ...1979518237
plasmid-determined 2-hydroxypyridine utilization by arthrobacter crystallopoietes.arthrobacter crystallopoietes has the ability to utilize 2-hydroxypyridine (2-hp) as a source of carbon and nitrogen and forms a blue extracellular pigment when grown in the presence of 2-hp. ultracentrifugal analyses of pigment producing (pig+) and pigment nonproducing (pig-) strains of a. crystallopoietes revealed the presence of plasmid material in both strains. recovery of plasmid dna from pig+ strains is two or three times greater than from pig- strains. the molecular weight of plasmid dna ...1979455149
control of morphogenesis in arthrobacter crystallopoiets: effect of cyclic adenosine 3',5'-monophosphate.the intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic amp) were measured at various intervals during growth and morphogenesis in arthrobacter crystallopoietes. cyclic amp levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. immediately after inoculation of spheres from glucose- to succinate-containing media, a 30-fold increase in intracellular cyclic amp was detected. this dramatic rise in cyclic amp preceded the observed chang ...1977190210
[dynamics of populations of microbial antagonists in nonsterile soil].the interaction between populations was studied with arthrobacter crystallopoietes and streptomyces olivocinereus, an actinomycete producing the antibiotic heliomycin active against gram-positive microorganisms. the two organisms were either cultivated together in a growth medium or the two populations were introduced simultaneously into nonsterile soil at different levels of population density. the antagonism was found in both cases: a. crystallopoietes cells died off when a population of the p ...19836843384
myceloid growth of arthrobacter globiformis and other arthrobacter species.transitory myceloid growth occurs in certain complex media with arthrobacter globiformis strain atcc 8010. this type of growth, however, was not observed in a medium which contained an array of metal ions but did not contain agents able to complex metal ions. addition of metal-complexing agents to this medium caused an interruption in the life cycle of strain 8010 so that growth occurred only as the myceloid form. it appeared that manganese was the critical metal that was removed by the metal-co ...19806254945
alteration of glucose metabolism of arthrobacter crystallopoietes by compounds which induce sphere to rod morphogenesis.succinate and several other compounds which induce sphere to rod morphogenesis of a. crystallopoietes were found to suppress both catabolism and assimilation of glucose. diauxic growth patterns resulted from growth on glucose plus any one of these compounds. glutamate stimulated growth but was not an inducer of morphogenesis. with this compound, diauxic growth and suppression of glucose catabolism or assimilation did not occur. glucose permease was studied with alpha-methylglucoside as substrate ...19695773009
intracellular substrates for endogenous metabolism during long-term starvation of rod and spherical cells of arthrobacter crystallopoietes.cells of arthrobacter crystallopoietes, harvested during growth as spheres and as rods, were starved by shaking at 30 c in phosphate buffer for 30 days, during which time they maintained 100% viability. changes in cellular components and the activity of specific enzyme pathways were monitored. a glycogen-like polysaccharide comprised 40% of the dry weight of growing spherical cells and 10% of the dry weight of rod cells. this material was utilized at approximately the same rate, on a percentage ...19705474876
isolation and characterisation of new gram-negative and gram-positive atrazine degrading bacteria from different french soils.the capacity of 12 soils to degrade atrazine was studied in laboratory incubations using radiolabelled atrazine. eight soils showed enhanced degradation of this compound. twenty-five bacterial strains able to degrade atrazine were isolated by an enrichment method from 10 of these soils. these soils were chosen for their wide range of physico-chemical characteristics. their history of treatment with atrazine was also variable. the genetic diversity of atrazine degraders was determined by amplifie ...200111451526
evolution of histamine oxidase activity for biotechnological applications.histamine is present to various degrees in many foods, and concentrations in fish samples are considered a good indicator of freshness and hygienic food quality. seeking for innovative methods to quantify histamine in foods, we used a synthetic gene designed on the sequence of histamine oxidase from arthrobacter crystallopoietes (hod) as the starting point in this study to develop a biosensor. hod was expressed in escherichia coli cells with a yield of ∼7 mg protein/l of fermentation broth. reco ...201423995223
draft genome sequence of arthrobacter crystallopoietes strain bab-32, revealing genes for bioremediation.arthrobacter crystallopoietes strain bab-32, a gram-positive obligate aerobic actinobacterium having potential application in bioremediation and bioreduction of a few metals, was isolated from rhizosphere soil of gandhinagar, gujarat, india. the draft genome (4.3 mb) of the strain revealed a few vital gene clusters involved in the metabolism of aromatic compounds, zinc, and sulfur.201323833141
description of tersicoccus phoenicis gen. nov., sp. nov. isolated from spacecraft assembly clean room environments.two strains of aerobic, non-motile, gram-reaction-positive cocci were independently isolated from geographically distinct spacecraft assembly clean room facilities (kennedy space center, florida, usa and centre spatial guyanais, kourou, french guiana). a polyphasic study was carried out to delineate the taxonomic identity of these two isolates (1p05ma(t) and ko_ps43). the 16s rrna gene sequences exhibited a high similarity when compared to each other (100 %) and lower than 96.7 % relatedness wit ...201323223813
the exposition of a calcareous mediterranean soil to toxic concentrations of cr, cd and pb produces changes in the microbiota mainly related to differential metal bioavailability.the involvement of the bacterial community of an agricultural mediterranean calcareous soil in relation to several heavy metals has been studied in microcosms under controlled laboratory conditions. soil samples were artificially polluted with cr(vi), cd(ii) and pb(ii) at concentrations ranging from 0.1 to 5000 mg kg(-1) and incubated along 28 d. the lowest concentrations with significant effects in soil respirometry were 10 mg kg(-1) cr and 1000 mg kg(-1) cd and pb. however, only treatments sho ...201222658943
application of alkaliphilic biofilm-forming bacteria to improve compressive strength of cement-sand mortar.the application of microorganisms in the field of construction material is rapidly increasing worldwide; however, almost all studies that were investigated were bacterial sources with mineral-producing activity and not with organic substances. the difference in the efficiency of using bacteria as an organic agent is that it could improve the durability of cement material. this study aimed to assess the use of biofilm-forming microorganisms as binding agents to increase the compressive strength o ...201222450795
electron microscopic study of cell surface rings during cell division and morphogenesis of arthrobacter crystallopoietes.the whole cell ultrastructure during cell division and morphogenesis of arthrobacter crystallopoietes was monitored using electron microscopic techniques. glucose-grown spherical cells were inoculated into succinate-based medium. in this medium, the organism undergoes a morphogenetic cycle consisting of elongation of spheres to rods, exponential growth as rods, and fragmentation of rods to spherical cells. raised bands or rings that encircled the cells were evident on the cell surface of both sp ...1977863857
hexavalent chromium reduction by an actinomycete, arthrobacter crystallopoietes es 32.environmental contamination by hexavalent chromium, cr(vi), presents a serious public health problem. this study assessed the reduction of cr(vi) by intact cells and a cell-free extract (cfe) of an actinomycete, arthrobacter crystallopoietes (strain es 32), isolated from soil contaminated with dichromate. both intact cells and cfe of a. crystallopoietes, displayed substantial reduction of cr(vi). intact cells reduced about 90% of the cr(vi) added within 12 h and cr(vi) was almost completely redu ...200414985627
plasmid localisation of atrazine-degrading genes in newly described chelatobacter and arthrobacter strains.abstract in a previous study, we isolated a collection of atrazine-degrading bacteria from various soils. the aim of this study was to localise the atrazine-degrading genes in these 25 atrazine-degrading strains. in the case of the gram-negative strains of chelatobacter heintzii, six to seven plasmids were observed. the atzabc and trzd genes were located on two or three plasmids with variable molecular masses. for the gram-positive strains of arthrobacter crystallopoietes, the atzbc genes were l ...200219709240
preliminary study of a microbeads based histamine detection for food analysis using thermostable recombinant histamine oxidase from arthrobacter crystallopoietes kait-b-007.we designed and prepared a micro biosensing system consisting of a flow through system with a sub-micro liter injection valve and a sub-micro liter volume bioreactor. an electrochemical detector was combined with the reactor for immediate detections. the volumes of the reactor and the sample loop for the injection were 850 nl and 320 nl, respectively. this paper described about the characteristics of the sensing system in the case of histamine detection for food analysis. histamine oxidase from ...200919064110
toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase.the hydantoinase process is applied for the industrial synthesis of optically pure amino acids via whole cell biocatalysis, providing a simple and well-established method to obtain the catalyst. nevertheless, whole cell approaches also bear disadvantages like intracellular degradation reactions, transport limitations as well as low substrate solubility. in this work the hydantoinase and carbamoylase from arthrobacter crystallopoietes dsm 20117 were investigated with respect to their applicabilit ...201728605882
calcite-forming bacteria for compressive strength improvement in mortar.microbiological calcium carbonate precipitation (mcp) has been investigated for its ability to improve the compressive strength of concrete mortar. however, very few studies have been conducted on the use of calcite-forming bacteria (cfb) to improve compressive strength. in this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. we isolated 4 cfb from 7 environmental concrete structures. using sequence analysis of the 16s rrna gen ...201020467254
chemical synthesis and enzymatic, stereoselective hydrolysis of a functionalized dihydropyrimidine for the synthesis of β-amino acids.a novel substrate, 6-(4-nitrophenyl)dihydropyrimidine-2,4(1h,3h)-dione (pno2phedu), was chemically synthesized and analytically verified for the potential biocatalytic synthesis of enantiopure β-amino acids. the hydantoinase (ec from arthrobacter crystallopoietes dsm20117 was chosen to prove the enzymatic hydrolysis of this substrate, since previous investigations showed activities of this enzyme toward 6-monosubstituted dihydrouracils. whole cell biotransformations with recombinant esc ...201526705241
c29 olefinic hydrocarbons biosynthesized by arthrobacter species.arthrobacter aurescens tc1, arthrobacter chlorophenolicus a6, arthrobacter crystallopoietes, and arthrobacter oxydans produce long-chain monoalkenes, predominantly cis-3,25-dimethyl-13-heptacosene. four other arthrobacter strains did not form alkenes. the level of cis-3,25-dimethyl-13-heptacosene in arthrobacter chlorophenolicus a6 remained proportional to cell mass during growth. cis-3,25-dimethyl-13-heptacosene did not support growth of a. chlorophenolicus a6.200919168653
a thermostable histamine oxidase from arthrobacter crystallopoietes kait-b-007.a thermostable histamine oxidase (ec 1.4.3.-) was found in cells of arthrobacter crystallopoietes kait-b-007 isolated from soil. the enzyme was purified about 715-fold over the cell free extracts with a yield of 55% by ammonium sulfate fractionation and various column chromatographies. the purified enzyme was homogeneous on polyacrylamide gel-electrophoresis (native-page). when the enzyme was kept at 65 degrees c and 70 degrees c for 10 min, the activity was fully stable at 65 degrees c and decr ...200416233600
utilization and degradation of imazaquin by a naturally occurring isolate of arthrobacter crystallopoietes.a species of bacteria that is capable of utilizing imazaquin as the sole carbon source was isolated from soil with repeated imazaquin applications, and was identified as arthrobacter crystallopoietes (designated as strain "wwx-1"). this isolate degrades imazaquin as high as 200 microg ml-1, and the estimated dissipation half-lives increased from 1.51 d for the treatment at 50 microg ml-1 to 4.75 d for 200 microg ml-1. optimal growth of wwx-1 in mineral salt medium with 50 microg ml-1 imazaquin w ...200717267013
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