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disseminated "mycobacterium genavense" infection in patients with aids.we describe 18 patients with advanced hiv infection, most of whom had a chronic illness characterised by fever, diarrhoea, and massive loss of weight. biopsy and necropsy samples revealed abundant acid-fast microorganisms in intestines, liver, spleen, lymph nodes, and many other tissues, which did not grow on solid media, although limited growth was observed in liquid blood cultures. using primers complementary to bacterial 16s rrna we amplified dna sequences from tissue and leucocyte extracts a ...19921352014
mycobacteriosis due to mycobacterium genavense in six pet birds.six cases of mycobacteriosis due to mycobacterium genavense in three budgerigars (melopsittacus undulatus), one orange-winged amazon (amazona amazonica), one flycatcher (cyanoptila cyanomelana), and one zebra finch (taeniopygia guttata) are discussed. gross lesions associated with the infection included a high degree of muscular wasting (five cases), hepatomegaly (four cases), and thickening of the wall of the small intestine (four cases). granulomas were found in the lung (one case) and the sub ...19938463407
[mycobacterium genavense infection in aids].fever, loss of weight, anaemia, hepatosplenomegaly and lymphadenopathy developed in two hiv-infected patients. at first malignant lymphoma with septicaemia was thought to be the cause. in both patients salmonella enteritidis was isolated by blood culture and found to be sensitive against the antibiotics that were given (5 g azlocillin and 2 g cefotaxime, three times daily each; additionally in case 2, metronidazole, 500 mg three times daily). because bone-marrow biopsy demonstrated acid-fast rod ...19938444111
mycobacterium genavense infection presenting as a solitary brain mass in a patient with aids: case report and review.patients with aids are prone to developing infections with opportunistic pathogens. recently, a new mycobacterium, mycobacterium genavense, has been found to cause infection in patients with aids. previously published reports indicate that patients who are infected with this organism present with the same clinical features as do patients with disseminated infection due to organisms of the mycobacterium avium complex. we describe an unusual case of a patient with aids who presented with grand mal ...19947888551
[mycobacterium genavense infections. incidence and clinical presentation]. 19957899377
laboratory aspects of "mycobacterium genavense," a proposed species isolated from aids patients."mycobacterium genavense" is a proposed new species recently reported to cause disseminated infections in 18 patients with aids in europe. we have recovered "m. genavense" as slowly growing fastidious mycobacteria in blood cultures of seven patients with aids. in the original studies of "m. genavense," the fastidious organism grew only in bactec 13a vials. the seattle, washington, isolates of "m. genavense" also failed to grow when subcultured from 13a vials to routine solid media, but dysgonic ...19921280652
disseminated mycobacterium genavense infection in two patients with aids.mycobacterium genavense is a recently defined fastidious organism that has been identified as a cause of disseminated infection in patients with aids. we report the cases of two patients who had advanced aids and a clinical syndrome of fever, anorexia, abdominal pain, diarrhea, and weight loss. in addition, splenomegaly and lymphadenopathy were prominent in both cases, and in one patient's case radiographic findings were suggestive of splenic abscesses. mycobacteria isolated from specimens of bl ...19947516719
mycobacterium genavense infections in pet animals.mycobacterium genavense, a recently reported cause of a wasting illness in patients with aids, was isolated from a cervical lymph mode from a dog with severe hind limb weakness and from trachael tissue from a parrot with acute onset respiratory distress. physicians caring for immunocompromised patients should consider birds and dogs potential sources of m. genavense infection and submit appropriate specimens for culture.19968784607
dapsone/pyrimethamine may prevent mycobacterial disease in immunosuppressed patients infected with the human immunodeficiency virus.dapsone exhibits activity against mycobacterium tuberculosis and mycobacterium avium complex (mac) in vitro. we retrospectively examined the incidence of mycobacterial diseases within a randomized prospective trial of prophylaxis for pneumocystis carinii pneumonia and toxoplasmosis. of 501 participants who had not previously had a mycobacterial disease, 274 received dapsone/pyrimethamine (200/75 mg once weekly) and 227 received aerosolized pentamidine (300 mg once every 4 weeks). the median cd4 ...19957742423
[disseminated mycobacterium genavense infection in patients with hiv infection. description of 5 cases and review of the literature].five cases of disseminated infection by mycobacterium genavense in patients with hiv infection are reported with a review of the literature.19969044636
mycobacterium genavense infection in canaries.a case of mycobacteriosis in a collection of canaries (serinus canarius) is described. the affected birds showed nonspecific clinical signs and macroscopic lesions (slight splenomegaly). histologic lesions found in liver, spleen, lungs, and kidneys consisted of noncaseous nodules containing accumulations of large macrophages that showed a highly vacuolated cytoplasm in which numerous acid-fast organisms were detected. attempts to isolate and culture the organisms using löwenstein-jensen and cole ...19968713044
bias of culture techniques for diagnosing mixed mycobacterium genavense and mycobacterium avium infection in aids.disseminated mycobacterium avium infection is a common complication in late-stage aids. we describe a patient seropositive for human immunodeficiency virus with a disseminated infection caused by mycobacteria. acid-fast rods were visible by microscopy in stool and bone marrow and in specimens from skin, liver, spleen, lung, and lymph nodes. using molecular biology techniques and standard culture techniques, we found evidence of a mixed mycobacterial infection with m. genavense and m. avium. we s ...19948195400
cultural studies on two isolates of "mycobacterium genavense" from patients with acquired immunodeficiency syndrome.two strains of the newly proposed species "mycobacterium genavense" have been isolated, using the radiometric system (bactec, becton dickinson), from the blood of two hiv-infected patients. disseminated infections due to the new organism closely resemble those of the mycobacterium avium complex, with prevalently intestinal symptomatology. we report here on the cultural behavior of the isolates, which are enhanced at ph 6, are inhibited by nap, do not require supplements, and grow best at 37 degr ...19948026160
etiological agents of mycobacterioses in pet birds between 1986 and 1995.between may 1986 and june 1995, mycobacteriosis was diagnosed by histology and microscopy in 204 (3.8%) of 5,345 necropsied pet birds. the predominant macroscopic changes were enlargement of the liver and spleen and thickening of intestinal walls. attempts to cultivate mycobacteria were made in 110 cases. acid-fact bacilli grew in 66 specimens (60%) only. in 18 cases we failed to obtain subcultures. therefore, species identification could be performed for only 48 isolates. identification was car ...19968815127
investigation on several phenotypic features in two strains of mycobacterium genavense.the newly recognized species mycobacterium genavense causes disseminated infections in aids patients, but its prevalence is difficult to assess because of its inability to grow on standard solid media. for the same reason, very little is known about the phenotypic traits of its isolates. we report here the results of our studies on two such strains isolated from aids patients and subcultured on a non-standard solid medium. besides several features conventionally explored for mycobacterial specia ...19947861987
utility of high-performance liquid chromatography for identification of mycobacterial species rarely encountered in clinical laboratories.high-performance liquid chromatography (hplc) has been demonstrated to be a suitable technique for determining the species of mycobacteria on the basis of their mycolic acid pattern. representative hplc profiles, which are needed for the visual recognition of chromatograms, have been published for the most frequently encountered mycobacterial species. no extensive study has been reported for less common species, and only a few, scattered chromatographic patterns are available in literature. this ...19957614969
[intestinal mycobacterial infections in aids. clinical course and treatment of infections caused by mycobacterium avium, mycobacterium kansasii, mycobacterium genavense].digestive apparatus is a common target of atypical mycobacteriosis in aids patients (at least 50% of patients with cd4+ lymphocytes < 50/mm3). we describe the clinical-histological features of two cases of whipple-like syndrome likely caused by mycobacterium avium (mai) (study performed by light and electron microscopy), of one case of infection caused by two morphological variants of a mai strain with a different sensitivity to antibiotics, of one case of m. kansasii infection and of two cases ...19947855386
mycobacterium genavense. autopsy findings in three patients.the authors report on the pathologic findings in three cases of disseminated infection with mycobacterium genavense, a recently described nontuberculous mycobacterium, in human immunodeficiency virus (hiv)-i-positive patients. the mycobacterium was identified by amplification of a 16s rdna gene fragment and subsequent sequence determination. the organs mainly involved were the small intestine, spleen, liver, and lymph nodes. in contrast, lungs, myocardium, and kidneys were not involved, or only ...19948279457
disseminated mycobacterium genavense infection: clinical and microbiological features and response to therapy.mycobacterium genavense is a newly described pathogen that causes disseminated infection in aids. it is difficult to detect and identify due to its slow growth and fastidious nature. there is little information available about therapy for this new pathogen. we describe clinical and laboratory features and response to therapy in four patients with advanced aids complicated by disseminated m. genavense infection from denver, colorado, usa.19938267909
invasive infection with mycobacterium genavense in three children with the acquired immunodeficiency syndrome.three children with human immunodeficiency virus infection and invasive infection with mycobacterium genavense are reported. fever spikes, abdominal cramps and distension, diarrhea or ileus, and anemia were the predominant symptoms in the severely immunodeficient patients (cd4 lymphocytes < 0.04 x 10(9)/l). numerous acid-fast bacilli were readily detectable by microscopy in stool samples and in lymph node biopsies, but cultures for mycobacteria remained negative. mycobacterium genavense should b ...19938462560
mycobacterium genavense sp. nov.strains of a suggested novel type of mycobacterium have been repeatedly isolated from patients with aids. we summarize the results of tests performed to determine enzymatic activities and metabolic properties, the results of fatty acid analyses, and the results of a comparative 16s rrna sequence determination. we propose formally that this organism represents a new species, mycobacterium genavense. the type strain is strain 2289, a culture of which has been deposited in the american type culture ...19938240965
the diagnostic mycobacteriology laboratory of the 1990s.the diagnostic mycobacterial laboratory of the 1990s must respond to a change in the clinical spectrum of mycobacterial infections brought about by an increase in the number of patients who are immunocompromised, are indigent, or have temporary or permanent implanted devices. emerging pathogens such as mycobacterium haemophilum and mycobacterium genavense, multidrug-resistant strains of mycobacterium tuberculosis, and catheter infections with rapidly growing mycobacteria are examples of new issu ...19938274611
mycobacterium genavense infection in a patient with aids who was successfully treated with clarithromycin. 19957548515
mycobacterium genavense: an emerging pathogen.disseminated mycobacterial infection, often due to mycobacterium avium complex, occurs frequently in patients with aids. more recently, a newly identified mycobacterium, mycobacterium genavense, has been repeatedly isolated from aids patients. mycobacterium genavense has unusual fastidious growth requirements and shows poor and variable growth in vitro. molecular biology techniques are necessary for accurate diagnosis of infection and have established mycobacterium genavense to be a definite cau ...19947698119
species-specific mycobacterium genavense dna in intestinal tissues of individuals not infected with human immunodeficiency virus.mycobacterium genavense species-specific dna was detected in intestinal tissues from two of nine individuals not infected with human immunodeficiency virus. this newly described microorganism is well documented as a causative agent of disseminated infections in aids patients. our results suggest that it may colonize the guts of individuals not infected with human immunodeficiency virus.19957494064
cervical lymphadenitis caused by a fastidious mycobacterium closely related to mycobacterium genavense in an apparently immunocompetent woman: diagnosis by culture-free microbiological methods.fastidious mycobacteria usually infect immunocompromised hosts (human immunodeficiency virus-infected or otherwise immunosuppressed patients). we here describe severe lymphadenitis, caused by a fastidious mycobacterium closely related to mycobacterium genavense, in an apparently immunocompetent woman, whose brother had died from an unidentified mycobacterial infection in 1969. a variety of techniques, including inoculation of nude mice, histopathology, electron microscopy, lipid analysis, atp me ...19958567903
endoscopic appearance of mycobacterium genavense: case report and review of the literature. 19968885356
disseminated mycobacterium genavense infection in canadian aids patients.mycobacterium genavense is a recently described mycobacterial species which thus far has been identified only in persons with advanced hiv disease. it appears to be a rare pathogen with an undefined reservoir. we describe the first two cases of m. genavense infection in canadian aids patients. the clinical presentation of fever and wasting with extremely low cd4 lymphocyte counts was indistinguishable from disseminated m. avium complex (mac) infection. however, blood cultures in bactec 13a mediu ...19957780101
mycobacterium genavense infection and survival. 19957575075
endoscopic appearance of mycobacterium genavense. 19979087851
mycobacterium genavense invasive infection in two children with aids: long-term followup.mycobacterium genavense is a rare cause of opportunistic infection in immunocompromised hosts. follow up of two cases of m. genavense invasive infection in children with haemophilia a and aids are presented. one patient died 18 months after diagnosis of m. genavense infection of an indirectly related cause, probably of pneumocystis carinii pneumonia. the second patient still attends our outpatient clinic and the infection is under control. both presented with abdominal lymphomas and pain and a w ...19958529716
growth-deficient mycobacteria in patients with aids: diagnosis by analysis of dna amplified from blood or tissue.amplification and sequencing of mycobacterial ribosomal rna genes (16s rdna) may permit the detection of growth-deficient species (i.e., those exhibiting no growth or those whose growth is delayed for more than 12 weeks). of blood samples from 26 patients with aids and a liver sample from one additional aids patient, three samples (two of blood and the one of liver) were positive by polymerase chain reaction only; cultures of these three samples remained negative for more than 12 weeks. analysis ...19957795072
clinical and epidemiologic features of infection with mycobacterium genavense. swiss hiv cohort study.to characterize clinical and epidemiologic features of infections with mycobacterium genavense.19957848023
mycobacteriosis caused by mycobacterium genavense in birds kept in a zoo: 11-year survey.we report on a disease in 27 birds (1 bird belonging to the order coraciiformes, 3 to piciformes, 4 to galliformes, 7 to psittaciformes, and 12 to passeriformes) caused by fastidious mycobacteria. all birds were caged at the antwerp zoo and died suddenly between 1983 and 1994. seventeen birds had no previous signs of disease, and 10 birds showed emaciation. gross necropsy findings were generally nonspecific, but all the birds were smear positive for acid-fast bacilli (afb). histopathologic evalu ...19968789007
splenectomy in a patients with aids, generalized mycobacterium genavense infection and severe pancytopenia. 19969039454
treatment of disseminated mycobacterium genavense infection. 19957662214
systemic infection with mycobacterium genavense following immunosuppressive therapy in a patient who was seronegative for human immunodeficiency virus.we describe, to our knowledge, the first case of disseminated mycobacterium genavense infection in a patient who was seronegative for human immunodeficiency virus. the patient, a 47-year-old woman, had been previously treated with immunosuppressive drugs for 9 months to control an unclassified immunologic disorder characterized by intermittent fever and inflammatory pulmonary, hepatic, and dermal infiltrates. antemortem and postmortem examinations revealed the presence of numerous mycobacteria i ...19979195092
inhibitory effects of polyoxyethylene stearate, panta, and neutral ph on growth of mycobacterium genavense in bactec primary cultures.we report on the influences of polyoxyethylene stearate (poes), panta, and ph on primary cultures of mycobacterium genavense in bactec vials. as a model for primary cultures from tissue, seven different strains first isolated from aids patients (five from switzerland and two from the united states) were inoculated into nude mice in order to obtain large amounts of bacilli to test different conditions simultaneously. our results demonstrate that the size of the inoculum (10[6] acid-fast bacilli/v ...19979350735
use of gas chromatographic fatty acid and mycolic acid cleavage product determination to differentiate among mycobacterium genavense, mycobacterium fortuitum, mycobacterium simiae, and mycobacterium tuberculosis.three mycobacterium genavense strains and three american type culture collection reference strains each of mycobacterium fortuitum, mycobacterium simiae, and mycobacterium tuberculosis were subcultured onto mycobacteria 7h11 agar (difco laboratories, detroit, mich.) supplemented with mycobactin j (allied laboratories, fayette, mo.). after 4 weeks of incubation at 37 degrees c in 10% co2, the cultures were analyzed by gas-liquid chromatography (glc) for their fatty acids and mycolic acid cleavage ...19989466781
treatment of nontuberculous mycobacterial infections: role of clarithromycin and azithromycin.the incidence of identification of nontuberculous mycobacteria has increased since the advent of the acquired immunodeficiency syndrome epidemic. although mycobacterium avium complex appears to be responsible for most episodes of nontuberculous disease, several other previously rare species are increasingly being detected, including mycobacterium kansasii, mycobacterium fortuitum/chelonei complex, and mycobacterium genavense. this review briefly summarizes the epidemiology and clinical features ...19979377608
[occurrence of mycobacterium genavense in birds].a total of 253 birds were investigated to determine the presence of mycobacteria. scrapings from various internal organs were stained according to ziehl-neelsen, and acid-fast bacilli were found in 26 birds (10.2%). cultivation of mycobacteria was attempted from 22 livers, 12 spleens, 14 kidneys, 12 lungs, and 9 intestines from these 26 birds. each sample was first decontaminated using a modified sodium dodecyl sulfate method, and three media were inoculated (bactec 12 b; löwenstein-jensen, herr ...19979411738
identification of mycobacterium genavense in intestinal tissue from a parakeet using two polymerase chain reaction methods: are pets a reservoir of infection in aids patients? 19979030378
cervical lymphadenitis caused by mycobacterium genavense in a healthy child. 19968852920
[infection with mycobacterium genavense in 2 hiv-seropositive patients in amsterdam]. 19989856239
routine rapid mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein gene (hsp65).to assess the utility of automated dna sequencing strategies for mycobacterium species assignment and surrogate rifampin susceptibility testing of mycobacterium tuberculosis complex isolates in a hospital-based clinical microbiology laboratory.19979278615
detection of fastidious mycobacteria in human intestines by the polymerase chain reaction.the aim of this study was to determine whether difficult-to-grow mycobacteria are present in human intestines. intestinal tissue samples were subjected to both mycobacterial culture and a polymerase chain reaction (pcr) assay. after detection by pcr, species identity was determined by hybridizing the amplified 16s rrna gene fragments with species-specific oligonucleotides. intestinal biopsies from 63 patients with noninflammatory bowel diseases (n = 22), crohn's disease (n = 31), or ulcerative c ...19979228475
[disseminated infection by mycobacterium genavense]. 19989887634
disseminated mycobacterium genavense infection as a cause of pseudo-whipple's disease and sclerosing cholangitis. 19979314476
[infection with mycobacterium genavense in 2 hiv-seropositive patients in amsterdam]. 199810028345
disseminated mycobacterium genavense infection in a patient with acquired immunodeficiency syndrome: first case report in taiwan.mycobacterium genavense is a recently described fastidious mycobacterium identified as a pathogen causing disseminated infection in patients with advanced human immunodeficiency virus (hiv) disease. in this report, we describe the first reported case of disseminated m. genavense infection in a patient with acquired immunodeficiency syndrome (aids) in taiwan. a 22-year-old chinese man was found to be seropositive for hiv at age 18, in 1993. in 1997, he presented with abdominal pain, weight loss, ...199910063276
genital infection due to mycobacterium genavense in a patient with aids. 19989868674
blood and charcoal added to acidified agar media promote the growth of mycobacterium genavense.ten different agar media were tested for the in vitro growth of mycobacterium genavense in primary cultures and in subcultures from bactec vials. these agar media were based on middlebrook 7h9, 7h10 and 7h11, and supplemented with additives: mycobactin j, yeast extract, charcoal, or defibrinated sheep blood. some media were acidified with phosphoric acid to a final ph of 6.2 +/- 0.2. fourteen m. genavense strains from nude mouse organs as well as one decontaminated clinical specimen (from a bird ...199910342107
differentiation of mycobacterium genavense and mycobacterium simiae by automated mycolic acid analysis with high-performance liquid chromatography.mycobacterium genavense, a fastidious opportunist in patients with aids, cannot be identified by conventional biochemical methods. computerized mycolic acid analysis by high-performance liquid chromatography offers an alternative that distinguishes the mycolic acid profile of m. genavense from those of all other organisms in the database developed at the centers for disease control and prevention.19968818899
rapid identification of mycobacterium genavense with a new commercially available molecular test, inno-lipa mycobacteria v2.we report a rare mesenteric localized mycobacterium genavense infection in a severely immunocompromised human immunodeficiency virus-infected patient. an inno-lipa mycobacteria v2 test was performed directly on biopsy samples. this new molecular tool could be used for simultaneous identification of mycobacterium species from human specimens, but other studies are needed to validate our first results.200415365056
disseminated infection with mycobacterium genavense: a challenge to physicians and mycobacteriologists.in the present study we compared the clinical presentations of patients with a clinical diagnosis of aids and disseminated mycobacterium genavense infection (n = 12) with those of patients with aids and disseminated m. avium complex (mac) infection (n = 24). abdominal pain was seen more frequently in the group of patients infected with m. genavense than in patients infected with mac (p = 0. 003). analysis of microbiological data revealed that stool specimens from patients infected with m. genave ...199910565904
standardized bactec method to measure clarithromycin susceptibility of mycobacterium genavense.a standardized clarithromycin susceptibility test for mycobacterium genavense is reported. the bactec radiometric broth dilution test method recommended for mycobacterium avium complex was modified to develop a reliable and reproducible procedure. test development involved optimization of medium ph and inoculum densities for antibiotic vials as well as growth control vials. mic control organisms included mycobacterium simiae, mycobacterium avium, and mycobacterium xenopi. growth control vials re ...19989508306
generalized mycobacterium genavense infection in hiv-infected patients: detection of the mycobacterium in hospital tap water.we describe 3 hiv-infected patients with disseminated m. genavense infection. the use of corticosteroids possibly favoured colonization and dissemination of atypical mycobacteria in these patients with low cd4 cell counts and may have masked symptoms of infection. the fact that these patients were treated with highly active antiretroviral therapy (haart) together with antimycobacterial therapy may explain that 1 patient was free from mycobacteria 16 months after the end of specific treatment. ho ...199910381220
bacteriologic investigations of unusual mycobacteria isolated from immunocompromised patients.mycobacterial isolates from blood and other extrapulmonary sites of six patients with aids were investigated because the isolates grew only in liquid media and failed to grow on solid culture media even on subculturing. our investigations indicated that these mycobacteria possess common, but unusual, characteristics and probably belong to an unrecognized species recently reported as "mycobacterium genavense."19938495588
mycobacterium genavense infection in a uk based patient with aids: diagnosis using molecular techniques. 19989924483
microaerophilic conditions promote growth of mycobacterium genavense.our studies show that microaerophilic conditions promote the growth of mycobacterium genavense in semisolid medium. the growth of m. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in bactec primary cultures (middlebrook 7h12, ph 6.0, without additives). by using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different m. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; ...19989705393
isolation of a specific dna fragment and development of a pcr-based method for the detection of mycobacterium genavense.the rise of mycobacterium genavense infections is making identification ever more important for diagnosis and treatment. moreover, isolation and identification of m. genavense are made difficult by the lack of growth on solid media and by its low generation rate in bactec liquid media. thus, amplification by pcr or similar techniques represents the only possibility of detecting and identifying m. genavense from tissue samples. in order to set up a simple and species-specific method based on the ...199910219597
hyperpigmentation in a patient with aids, receiving rifabutin for disseminated mycobacterium genavense infection. 19989686833
estimation of the spread of pathogenic mycobacteria in organic broiler farms by the polymerase chain reaction.organic poultry breeding allows for increased exposure of birds to soil, faeces, and wildlife, which have been associated with the transmission of mycobacterial infections. therefore the aim of this study was to investigate the spread of the major pathogenic mycobacteria in organically reared broilers in greece using a diagnostic algorithm that relied on a combination of the polymerase chain reaction (pcr) and the restriction fragment length polymorphism analysis (rflp). liver, spleen and gonads ...200918774661
[mycobacterium genavense infection in 2 hiv seropositive patients in amsterdam].two patients with an hiv-i infection, a man aged 47 with confusion, aphasia and diarrhoea, and a man aged 32 with dysphagia, a non-productive cough and diarrhoea, were diagnosed as having a disseminated mycobacterium genavense infection. both had low counts of cd4+ t lymphocytes. they responded to antimycobacterial treatment. m. genavense was recognized in geneva in the early nineties as a causative agent of disseminated mycobacterial infections in hiv-seropositive patients with poor cellular im ...19989623172
mycobacterium genavense infection in two aged ferrets with conjunctival lesions.mycobacterium genavense infection was diagnosed in two adult ferrets. disseminated mycobacteriosis was diagnosed in a castrated 5-year-old sable ferret with generalised peripheral lymph node enlargement and a proliferative lesion of the conjunctiva of the nictitating membrane. the diagnosis was based on characteristic cytology and sequence analysis of the 16s rrna gene amplified using the polymerase chain reaction from fresh biopsy material. therapy with rifampicin, clofazimine and clarithromyci ...200011098383
mycobacteriosis in birds.avian mycobacteriosis is an important disease which affects companion, captive exotic, wild and domestic birds. the disease is most commonly caused by mycobacterium avium and mycobacterium genavense. lesions are typically found in the liver and gastrointestinal tract, although many other organ systems can potentially be affected. the authors review those species of mycobacterium reported to affect birds, the epidemiology of avian mycobacteriosis, immunological responses to mycobacterial infectio ...200111288511
disseminated mycobacterium genavense in an hiv-infected patient from south america. 200312757055
real-time polymerase chain reaction testing for the detection of mycobacterium genavense and mycobacterium avium complex species in avian samples.diagnosis of avian mycobacteriosis, caused by mycobacterium genavense or species belonging to the mycobacterium avium complex (mac), is problematic. polymerase chain reaction (pcr) offers rapid and sensitive detection of minute quantities of dna, and conventional protocols have been used for evaluating avian specimens. the recent development of real-time pcr offers several advantages over conventional pcr. in attempts to improve diagnosing avian mycobacteriosis, a real-time taqman pcr assay was ...200314708989
comparison of four rapid dna extraction techniques for conventional polymerase chain reaction testing of three mycobacterium spp. that affect birds.mycobacteriosis is an avian disease that is most commonly caused by mycobacterium avium or mycobacterium genavense. in order to optimize molecular laboratory tests for diagnosing mycobacteriosis in birds, we compared four methods of rapid dna extraction with isolates of m. avium, m. genavense, and mycobacterium fortuitum. dna extraction methods included enzymatic lysis, boiling for 30 min followed by enzymatic lysis, four cycles of freezing and thawing followed by enzymatic lysis, and bead beati ...200314709001
mycobacterium genavense and cutaneous disease in aids. 19989490604
nontuberculous mycobacterial immune reconstitution syndrome in hiv-infected patients: spectrum of disease and long-term follow-up.the long-term outcome and spectrum of disease of nontuberculous mycobacterial immune reconstitution syndrome have not been described.200516231262
[infection with mycobacterium genavense in 2 hiv-seropositive patients in amsterdam]. 19989763851
detection and differentiation of mycobacterium avium and mycobacterium genavense by polymerase chain reaction and restriction enzyme digestion analysis. 200010690777
[diagnosis of mycobacterium genavense infection by inno-lipa mycobacteria amplification v2].diagnosis of atypical mycobacterial infections can be facilitated by molecular biology techniques.200616783260
mycobacterium genavense infection in aids: imaging findings in eight patients.this retrospective study included eight hiv-positive patients with a m. genavense infection. seven of these patients had a ct scan of the abdomen and a us examination, whereas one patient with pulmonary symptoms had conventional chest radiographs and thin-section ct scan of the thorax. multiple large retroperitoneal and mesenteric lymph nodes were demonstrated in seven patients; low-attenuation centers within enlarged nodes were identified in two patients. on ct scans two cases showed circumfere ...200111218013
hyperammonemia in generalized mycobacterium genavense infection after renal transplantation.after solid organ transplantation, patients are susceptible to infection caused by uncommon pathogens due the immunosuppressive drug therapy. here, we report the first case of disseminated mycobacterium genavense infection in a hiv seronegative renal transplant patient. the most striking clinical feature was a decreased consciousness. blood results revealed hyperammonemia with otherwise normal liver function. occurrence of hyperammonemia and massive m. genavense infection has not been reported b ...200717250553
mycobacterium genavense in aids patients, report of 24 cases in italy and review of the literature.mycobacterium genavense is a frequently missed agent of disseminated disease in aids patients. the increasing frequency with which such organism is being isolated in italy suggested a comparison of local survey with data reported in literature. isolates presumed to belong to the species m. genavense were centralized and identified by means of genomic sequencing and/or hplc analysis of cell wall mycolic acids; clinical data were obtained from relevant patients' record and collected using a proper ...19989663512
gamma interferon is essential for clearing mycobacterium genavense infection.factors determining the in vivo replication of the opportunistic pathogen mycobacterium genavense are largely unknown. following intravenous injection of a patient isolate, m. genavense could not be recovered by culture or detected by pcr in the livers or spleens of infected balb/c mice. in contrast, m. genavense was found to chronically persist and multiply in the livers and spleens of intravenously infected syngeneic gamma-interferon-gene-deficient (gko) mice as evidenced by acid-fast stains o ...200010816534
mycobacterium genavense infection in a patient with long-standing chronic lymphocytic leukaemia.we describe the first case of disseminated infection with mycobacterium genavense in an hiv-seronegative patient with a chronic haematological disorder. our patient, an 80-year-old woman, had been under long-term treatment with chlorambucil (partially in combination with prednisone) for b-cell chronic lymphocytic leukaemia (b-cll). when she developed general fatigue and progressive anaemia, as well as progressive lymphadenopathy and splenomegaly, bone marrow biopsy revealed granulomas with acid- ...200011086646
molecular characterization of environmental mycobacterium strains by pcr-restriction fragment length polymorphism of hsp65 and by sequencing of hsp65, and of 16s and its1 rdna.fifteen mycobacterial strains from the environment, not clearly identifiable by biochemical properties, were analyzed with molecular markers: pcr-restriction enzyme analysis of hsp65 and sequencing of hsp65, and of the internal transcribed spacer 1 (its1) and 16s rdna. the 16s rdna sequencing closely related the strains to a slow-growing mycobacterial group including mycobacterium simiae, mycobacterium lentiflavum, mycobacterium genavense, mycobacterium triplex and mycobacterium heidelbergense. ...200011081578
granulomatous pneumonia caused by mycobacterium genavense in a dwarf rabbit (oryctolagus cuniculus).a juvenile dwarf rabbit (oryctolagus cuniculus) with clinical signs of dyspnea and suspected ascites was submitted for necropsy. the main macroscopic findings were a watery red pleural effusion and some whitish striated foci in the lungs. in addition, there were multifocal scars in the cortex of the kidneys. the histologic examination of the lungs showed a severe granulomatous pneumonia with detection of acid-fast bacilli, in the kidneys, an interstitial chronic lymphoplasmacellular nephritis wi ...200919429993
mycobacterium genavense duodenitis following allogeneic peripheral blood stem cell transplantation.a 56-year-old man was diagnosed with mycobacterium genavense duodenitis 21 months after an allogeneic peripheral stem cell transplant complicated by graft-versus-host disease requiring intense immunosuppression. this duodenitis responded to prolonged therapy with clarithromycin, ciprofloxacin, and rifabutin and reduction of immunosuppression.200919656345
treatment of disseminated mycobacterium genavense infection in a murine model with ciprofloxacin, amikacin, ethambutol, clarithromycin and rifabutin.mycobacterium genavense is a recently described agent which can induce disseminated infections in patients with aids. up to now, no standard approach to treatment has been defined and patients have been treated empirically with antibiotics used for treating infections caused by other nontuberculous mycobacteria. in this study, we compared the effectiveness of ciprofloxacin, amikacin, ethambutol, clarithromycin and rifabutin in the treatment of an animal model of m. genavense infection in c57bl/6 ...19989818747
prolonged incubation of blood and bone marrow cultures in 12b bottles processed on the bactec 460 tb system does not increase microbial recovery.standard references continue to recommend testing specimens processed on the bactec tb system for 8 weeks, despite evidence that mycobacteria are rarely recovered beyond 5-6 weeks. to clarify this issue, we retrospectively reviewed all positive blood/bone marrow cultures processed during a 17-month period when specimens were tested for 6 weeks. we then prolonged the incubation period to 8 weeks during the subsequent 5 months. excluding mycobacterium genavense, only 1 of 159 mycobacterial isolate ...19968902405
education and imaging. gastrointestinal: mycobacterium genavense enteritis in an immunosuppressed patient. 200919702915
localized mycobacterium genavense soft tissue infection in an immunodeficient hiv-negative patient.an hiv-negative woman with chronic lymphopenia related to past sarcoidosis situated in the bone marrow presented with an inflammatory lesion in the iliac region due to a localized mycobacterium genavense soft tissue infection. the lesion resolved after 12 months of antibiotic therapy with clarithromycin, ethambutol and ciprofloxacin. the patient had no recurrence of the subcutaneous abscess during a follow-up period of 14 months after the end of the treatment.200010706181
disseminated infection with mycobacterium genavense in a grizzled giant squirrel (ratufa macroura) associated with the isolation of an unknown mycobacterium.disseminated infection with mycobacterium genavense was diagnosed in an adult grizzled giant squirrel (ratufa macroura). microscopical examination showed granulomatous inflammation in the brain, kidneys, lungs and maxilla with intracellular acid-fast bacilli. m. genavense and a novel species of mycobacterium (proposed name 'mycobacterium lipsiensis') were identified.201020153866
mycobacterium genavense enteritis in a patient with aids: radiologic features. 199910587172
mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species.'mycobacterium sherrisii' is an undescribed species that appears to be emerging, in particular, among hiv-positive patients originating from africa. to describe 'm. sherrisii', to ensure that the species name is validly published and to define its phylogenetic position, we collected 11 of these strains reported in five previous studies, and subjected them to biochemical identification, cell-wall mycolic acid analysis and sequencing of multiple housekeeping genes. the bacteria formed smooth and g ...201020639227
winnipeg conference. what do the numbers mean?presentations at the fifth annual conference on hiv/aids research in winnipeg (canada) considered the level of viral load and cd4+ counts that can be used to guide therapeutic decisions. discussions centered on the dangers of relying too heavily on any one measurement of an individual's viral load, and other topics, such as what happens when trial dropouts all have low cd4+ counts, when to worry about patients with 650 t cells, and why multidrug therapy is the least inadequate option. among the ...199511362723
infection with mycobacterium genavense in a patient with systemic lupus erythematosus.a 55-year-old woman with systemic lupus erythematosus was admitted with fever of unknown origin. she had been on an immunosuppressive regimen for the past 8 years including steroids and azathioprine. laboratory parameters revealed a markedly elevated c-reactive protein of 189 mg/l, antinuclear antibodies of 1:2,560, a hemoglobin level of 9.0 g/dl, and a severe lymphopenia (total lymphocytes 49.4/microl, cd4(+) cells 2/microl, cd8(+) cells 7/microl). neither blood culture samples nor computed tom ...200919252819
disseminated nontuberculous infections with mycobacterium genavense during sarcoidosis.sarcoidosis is a chronic disease characterised by the development and accumulation of granulomas in multiple organs. we report two observations of disseminated mycobacterium genavense infection in patients with proven sarcoidosis. high fever and abdominal pain appeared at 8 and 18&emsp14;months following the initiation of immunosuppressive therapy. abdominal computed tomography scans of the patients showed diffuse mesenteric lymphadenitis and splenomegaly. the diagnosis was obtained on bone marr ...200920956155
disseminated mycobacterium genavense infection in a fiv-positive cat.an 8-year-old fiv-positive australian cat was presented with coughing, periocular alopecia, pyrexia and inappetence. skin scrapings demonstrated demodex cati mites. antibiotics were administered and it was treated successfully for periocular demodectic mange, but the cat continued to exhibit respiratory signs and lose weight. further investigation revealed an ascarid infection and active chronic inflammation of undetected cause affecting the lower airways. repetitive treatment with pyrantel fail ...199911919012
[uncommon mycobacterial infections in domestic and zoo animals: four cases with special emphasis on pathology].infections caused by classical tubercle bacilli are rare during the last years. nevertheless, diseases caused by other mycobacteria have to be considered clinically and in diagnostic pathology especially in cases of immunosuppression and due to their potential zoonosis risk. an infection by mycobacteria was diagnosed in four animals (mayotte maki, blue-headed parrot, patagonian sealion, beagle) necropsied between 1995 and 2002 in the institute of veterinary-pathology of the university of leipzig ...200314560447
rapid diagnosis of mycobacterium genavense disseminated infection by the microseq 500: a case report in a two year old hiv-negative child.this paper evaluates the capability of microseq 500 instrument to improve the diagnosis of mycobacterium genavense the strain was isolated from a two year old child admitted to our hospital for hepatosplenomegaly and massive abdominal lymphadenopathies. dna was extracted from a lymph node and examined by amplifying 500 bp at the 5' end of 16s rrna gene using microseq 500 16s rdna bacterial identification pcr kit. sequencing reactions were performed with microseq 500 16s rdna bacterial identifica ...201627650624
mycobacterium genavense and avian polyomavirus co-infection in a european goldfinch (carduelis carduelis).systemic mycobacteriosis associated with avian polyomavirus infection was diagnosed histologically in an 8-year-old, captive european goldfinch with a history of nervous signs. severe mycobacterial lesions were observed in the central nervous system, lungs, cervical air sacs and adrenal glands, without involvement of the gastrointestinal tract. in addition to mycobacteriosis, intranuclear inclusions, typical of polyomavirus, were identified in the adrenal glands. polymerase chain reaction assays ...200717899468
mesenterial involvement of mycobacterium genavense infection: hard to find, hard to treat.mycobacterium genavense is a rare pathogen affecting severely immunosuppressed patients. we report the case of persistent relapsing m. genavense infection in a 48-year-old african man with a positive diagnosis of hiv infection. despite being under effective antiretroviral therapy with partial immune reconstitution, he developed irreversible long-term abdominal complications, possibly due to persistent m. genavense infection and sustained inflammation. case management consists of individual risk ...201525976189
disseminated mycobacterium genavense infection in a patient with immunosuppressive therapy and lymphoproliferative malignancy.we present the case of a 51-year-old woman who presented a right supraclavicular mass corresponding to lymphadenitis with atypical mycobacterium (mycobacterium genavense). later, she developed subcutaneous purulent small nodules containing the same germ. the presence of this mycobacterium was in the context of recurrent peripheral t-cell lymphoma and immunosuppressive therapy for seronegative inflammatory polyarthritis.201424724760
[isolation and identification of mycobacterium bovis and non-tuberculous mycobacteria in raw milk samples in mersin province].this study was aimed to isolate and identify mycobacterium bovis and non-tuberculous mycobacteria (ntm) species in raw milk samples from cattles and to compare the diagnostic performance of the methods used for that purpose. a total of 145 raw milk samples from cattles were collected from five villages in mersin province (located on mediterrenean region of turkey) between april and june 2008. presence of mycobacteria was investigated by ehrlich ziehl neelsen (ezn) staining method, culture in löw ...201222639317
disseminated mycobacterium genavense infection in a healthy boy.mycobacterium genavense (m genavense) has been recognized as a life-threatening pathogen in severely immunocompromised patients. to our knowledge, disseminated m genavense infection has never been described in immunocompetent individuals. here, we report a case of disseminated m genavense infection in a healthy japanese boy. a 15-year-old boy who had never been diagnosed with an immunodeficiency disorder was hospitalized because of ileus. tumorous lesions were identified in the ileum, cecum, and ...201020655091
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