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[acid-stable and acid-unstable alpha-amylases of the mold fungi aspergillus].acid-sable alpha-amylase of asp. niger and acid-unstable, alpha-amylase of asp. oryzae were studied. it was demonstrated, that beside being a more acid-stable properties, alpha-amylase asp. niger has increased thermal stability as compared to alpha-amylase asp. oryzae. the molecular weights of acid-stable alpha-amylase and acid-unstable alpha-amylase are 58 000 and 51 000, respectively. the amino acid composition, and the c- and n-terminal amino acids of both forms of alpha-amylases were determi ...197831203
serological evidence of bluetongue in game animals in botswana.using the agar gel precipitin technique the sera of 397 african buffalo (syncerus caffer) and 90 sera of other common game species were examined for bluetongue antibodies. of the adult buffalo 283 out of 325 (87 per cent) were positive. buffalo calves were positive in 25 out of 72 cases (35 per cent). positive reactions were also recorded in lechwe (kobus leche), tsessebe (damaliscus lunatus), red hartebeeste (alcelaphus buselaphus), gemsbok (oryx gazella), sable (hippotragus niger) and impala ( ...1978204086
application of ovine luteinizing hormone (lh) radioimmunoassay in the quantitation of lh in different mammalian species.a sensitive double antibody radioimmunoassay has been developed for measuring luteinizing hormone (lh) in various african mammalian species, using rabbit anti-ovine lh serum (gdn 15) and radioiodinated rat lh or ovine lh. serum and pituitary homogenates from some african mammals (hyrax, reedbuck, sable, impala, tsessebe, thar, spring-hare, ground squirrel and cheetah, as well as the domestic sheep, cow and horse and laboratory rat and hamster) produced displacement curves parallel to that of the ...1977330153
a doubtful relationship between tyrosine trna and suppression of the vermilion mutant in drosophila.the conditions under which drosophila melanogaster are grown markedly influence the amount of the hypermodified nucleoside q found in certain trnas. this effect on q biosynthesis was found in both the wild-type and the suppressor of sable [su(s)2] mutant. suppressed vermilion flies [su(s)2v; bw] with 78% of the tyrosine trna in the q-lacking (gamma) form had brown eyes indistinguishible from su(s)2v; bw flies with only 6% of the tyrosine trna in the gamma form. the proposal that this trna is a s ...1977413100
investigations on the prevalence of trypanosome carriers and the antibody response in wildlife in northern botswana.605 buffalo, 60 lechwe, 23 kudu, 23 impala, 15 tsessebe, 22 sable and two reedbuck from northern botswana were examined for trypanosome infections by serological and/or parasitological means. the indirect fluorescent antibody test (ifat), the haematocrit centrifugation technique (hct) and rodent subinoculation were used. the overall prevalence of patent infection with trypanosomes in buffalo, lechwe and reedbuck was 15,7%. in the case of buffalo and lechwe it was possible to classify their origi ...1978675844
gene transfer in drosophila melanogaster: cytological alterations in the salivary chromosomes of transformed stocks.cytological abnormalities are observed in salivary chromosomes of stocks of drosophila melanogaster possessing dna-induced, v+ transformations mapping either at 1-0.0 or 1-33.0. in initial studies, the untransformed control stock and six transformed stocks were assigned code numbers prior to cytological examination. salivary chromosome regions 1b9 10--c 2-3 and 10a1 2--10b1-2 were carefully examined in each of the coded stocks. (band 1b11 is tentatively identified as the site of the suppressor-o ...1975807461
blood parasites of some wild bovidae in botswana.blood smears from a total of 282 wild bovidae from ngamiland, botswana were examined for the presence of blood parasites. the following species were sampled: 190 african buffalo, 23 impala, 10 blue wildebeest, 18 tsessebe, 1 eland, 13 lechwe, 16 kudu and 11 sable antelope. in addition, blood from 36 of the above antelope and from a further 48 buffalo was inoculated into rodents to test for the presence of trypanosomes. an anaplasm morphologically indistinguishable from anaplasma marginale theile ...1975813170
analogs of natural lipids. iii. nonequivalence of methyl groups in methylated phospholipids.the dimethyl esters of a series of diastereoisomeric cyclopentanoid analogs of phosphatidic acid (a.j. hancock, m.h. stokes, and h.z. sable. 1977. j. lipid res. 18: 81-92.) have been studied by proton nmr spectroscopy at 60, 100, and 300 mhz. the signals of the p-o-ch3 protons near delta 3.80 show the expected doubling due to the 31p-1h coupling. in addition, the spectra of three of the isomers show additional multiplicity the line separation (in hz) being proportional to the frequency of the sp ...1977833508
in vitro and in vivo antibacterial activity of t-1220, a new semisynthetic penicillin.t-1220, sodium 6-[d-(-)-alpha-(4-ethyl-2,3-dioxo-1-piperazinylcarbonyl-amino)- alpha-phenylacetamido] penicillanate, is a new semisynthetic penicillin derivative that possesses a broad spectrum of in vitro antibacterial activity against gram-positive and gram-negative bacteria. t-1220 is more effective than carbenicillin (cb-pc) against pseudomonas aeruginosa, klebsiella pneumoniae, proteus species, and serratia marcescens. addition of human serum to culture media did not significantly alter the ...1977921239
[skin regeneration in several species of mammals].the healing of full-thickness wounds (1.2 cm2) was studied in minks and sables. the wound closure in both animal species resulted mainly from the contraction of the wound. a small regenerate containing hair and sebaceous glands was formed in the centre of the wound. it is supposed that these hairs developed from the old hair roots transfered with the lower layers of the corium adjoining the wound.1976953317
[natural and induced ovulation in sables (martes zibellina l)]. 19761034552
[estrogens in the reproduction process in sables]. 19751156615
[progestin and estrogen concentrations during the reproduction of sables].competitive protein-binding analysis and radioimmune method was applied to the study of the content of progestins and estrogens in the blood and urine in female sables of different age (kept in cages) at periods corresponding to the estrus, diapause and true pregnancy. sables with a normal course of pregnancy displayed a regular increase in the progestin level already at the period of diapause, although relatively small in value. the period of true pregnancy is attended by a pronounced elevation ...20161228756
[growth and hormonal conditions of maturation of sables]. 19761253684
modifiers of bx1 alter the distribution of ubx proteins in haltere imaginal discs of drosophila.the bithorax (bx) mutations in the ultrabithorax (ubx) gene of drosophila melanogaster cause homeotic transformations of anterior third thoracic structures (t3a) toward anterior second thoracic structures (t2a) in the adult fly. a corresponding loss of ubx protein expression in t3a of bx imaginal discs has been observed (white and wilcox, 1985). we describe two genetic loci which modify the bx-induced transformation. a locus which we map very close to the pink peach (pp) gene suppresses the bx1 ...19921601188
splicing of retrotransposon insertions from transcripts of the drosophila melanogaster vermilion gene in a revertant.a mutation of the drosophila melanogaster vermilion (v) gene known as v1 is caused by the insertion of a 412 retrotransposon into the 5' untranslated region of the first exon. mutants carrying this insertion accumulate a low level of mrna from which most of the transposon sequences have been eliminated by splicing at cryptic sites within transposon sequences. here, we demonstrate that a revertant of the v1 allele called v+37 is caused by the insertion of a second retrotransposon, the b104/roo el ...19911664404
the drosophila suppressor of sable gene encodes a polypeptide with regions similar to those of rna-binding proteins.the nucleotide sequence of the drosophila melanogaster suppressor of sable [su(s)] gene has been determined. comparison of genomic and cdna sequences indicates that an approximately 7,860-nucleotide primary transcript is processed into an approximately 5-kb message, expressed during all stages of the life cycle, that contains an open reading frame capable of encoding a 1,322-amino-acid protein of approximately 150 kda. the putative protein contains an rna recognition motif-like region and a high ...19911703632
mutations in the su(s) gene affect rna processing in drosophila melanogaster.we have studied the effect of mutations in the suppressor of sable [su(s)] gene on p element-induced yellow alleles. two independent mutations tested, y76d28 and y1#7, contain a 1.1-kilobase (kb) p element inserted in the 5' transcribed untranslated portion of the yellow gene. sequences responsible for the y1#7 mutation are inserted in the same transcriptional orientation as yellow and cannot be processed by splicing, and this mutation is not suppressed by su(s) mutations. p element sequences ar ...19911714588
non-invasive assessment of the incidences of pregnancy and pregnancy loss in the feral horses of sable island.field observations of 400 totally unmanaged feral horses on sable island, nova scotia, were complemented by oestrogen determinations in faecal samples from 154 identified females over a 4-year period (454 mare-years). of mares that were sampled throughout the year and subsequently produced foals, 92.1% exhibited elevated faecal oestrogens between 15 october and 30 march. the results confirm that faecal oestrogens are a useful indicator of pregnancy after approximately 120 days gestation. distrib ...19911795292
present status of trichinellosis in japan.there have been three mass outbreaks of human trichinellosis in japan. the first was in 1974 in iwasaki, aomori prefecture, involving a group of hunters who ate raw meat of a black bear they had shot. of the 20 people who ate the bear meat raw, 15 showed clinical symptoms of trichinellosis. the second outbreak was experienced in sapporo, hokkaido, in 1980. the patients had eaten the raw meat of a brown bear served in a local restaurant. twelve people were diagnosed positive on the basis of clini ...19911822910
mobile element insertions causing mutations in the drosophila suppressor of sable locus occur in dnase i hypersensitive subregions of 5'-transcribed nontranslated sequences.the locations of 16 mobile element insertions causing mutations at the drosophila suppressor of sable [su(s)] locus were determined by restriction mapping and dna sequencing of the junction sites. the transposons causing the mutations are: p element (5 alleles), gypsy (3 alleles), 17.6, hms beagle, springer, delta 88, prygun, stalker, and a new mobile element which was named roamer (2 alleles). four p element insertions occur in 5' nontranslated leader sequences, while the fifth p element and al ...19901963868
relation of cortical cell orientation selectivity to alignment of receptive fields of the geniculocortical afferents that arborize within a single orientation column in ferret visual cortex.neurons in the primary visual cortex of higher mammals are arranged in columns, and the neurons in each column respond best to light-dark borders of particular orientations. the basis of cortical cell orientation selectivity is not known. one possible mechanism would be for cortical cells to receive input from several lateral geniculate nucleus (lgn) neurons with receptive fields that are aligned in the visual field (hubel and wiesel, 1962). we have investigated the relationship between the arra ...19912027051
an outbreak of babesiosis in imported sable antelope (hippotragus niger).a complete necropsy performed on 2 sable antelope (hippotragus niger), revealed lesions concomitant with a massive haemolytic crisis. these included widespread oedema and anaemia of the carcass, severe oedema of the lungs, petechiae and echymoses of the epicardium, a moderate splenomegaly and a severe haemoglobinuria. the histopathological lesions included a moderate alveolar oedema, the presence of haemosiderin in the spleen and lymph nodes, and mild degenerative changes of the renal tubular ep ...19912051446
albino gene dosage and retinal decussation patterns in the pigmented ferret.we have examined the retinal decussation patterns in pigmented ferrets that were either wild-type sable or heterozygous with one albino gene. unilateral injections of horseradish peroxidase were made into the optic tract and labeled ganglion cells visualized in retinal wholemounts. in both wild-type and heterozygous ferrets, those ganglion cells in the temporal retina with the largest cell bodies projected only to the contralateral side of the brain. the total number of ipsilaterally projecting ...19912059573
structure and transcription of the drosophila melanogaster vermilion gene and several mutant alleles.the nucleotide sequence and intron-exon structure of the drosophila melanogaster vermilion (v) gene have been determined. in addition, the sites of several mutations and the effects of these mutations on transcription have been examined. the major v mrna is generated upon splicing six exons of lengths (5' to 3') 83, 161, 134, 607, 94, and 227 nucleotides (nt). a minor species of v mrna is initiated at an upstream site and has a 5' exon of at least 152 nt which overlaps the region included in the ...19902108317
a retrotransposon 412 insertion within an exon of the drosophila melanogaster vermilion gene is spliced from the precursor rna.three alleles of the drosophila melanogaster vermilion (v) gene are suppressed by recessive mutations at the suppressor of sable [su(s)], gene. previous work has established that these alleles have identical insertions of the 412 retrotransposon in the 5'-untranslated region of the gene. despite the transposon insertion in an exon, v mutants accumulate trace amounts of apparently wild-type-sized transcripts in a su(s)+ background, and the level of v transcript accumulation is increased by su(s) ...19902163342
dorothea dix and her two missions of mercy in nova scotia.dorothea lynde dix, 19th century defender of the rights of the mentally ill to hospital care, holds a significant place in the history of nova scotia. her two major accomplishments in this province include laying the groundwork for its first psychiatric hospital and the outfitting of treacherous sable island with rescue equipment to aid ships stranded off its shore. the techniques that miss dix employed in these missions parallel those she successfully used in the establishment of psychiatric ho ...19902180553
purkinje cell simple spike activity during grasping and lifting objects of different textures and weights.1. three monkeys (2.5-3.5 kg) were trained to pinch an object between the thumb and forefinger and to lift it a vertical distance of 1.0-2.0 cm. either the object weight (15, 65, or 115 g) or the surface texture (sand paper or polished metal) contacting the fingers could be varied. the object was equipped with a vertical position transducer, an accelerometer, and strain gauges that measured the grip force and the vertical load force. 2. in accordance with similar previously published studies on ...19902230918
effects of pasteurella haemolytica leukotoxin on neutrophils from white-tailed deer and several exotic ruminant species.pasteurella haemolytica leukotoxin is a pore-forming cytolysin which acts as a virulence factor in pasteurellosis of domestic ruminants. leukocytes from cattle, sheep and goats are susceptible to leukotoxin-induced lysis; however, leukocytes from non-ruminant species so far tested are resistant to leukotoxin-induced lysis. neutrophils obtained from three white-tailed deer, four saiga antelope, an addra gazelle, a grant's gazelle and a sable antelope were tested for susceptibility to the lytic ef ...19902382402
molecular and genetic organization of the suppressor of sable and minute (1) 1b region in drosophila melanogaster.recessive mutations at the suppressor of sable [su(s)] locus in drosophila melanogaster result in suppression of second site mutations caused by insertions of the mobile element 412. in order to determine whether su(s) mutations might have other phenotypes, a saturation mapping of the su(s) region was carried out. the screen yielded 76 mutations that comprise ten genetic complementation groups ordered distal to proximal as follows: l(1)1bh, l(1)1bi, m(1)1b, su(s), l(1)1bk, l(1)1ca, mul, tw, l(1) ...19892503417
experimental infection of eland (taurotrages oryx), sable antelope (ozanna grandicomis) and buffalo (syncerus caffer) with foot-and-mouth disease virus.the course of experimental infection of a type sat 1 fmdv strain was studied in buffalo, sable antelope and eland following tongue inoculation and contact and has been compared with that in cattle. all species became infected, although disease was less severe in the game animals and larger amounts of virus were required to infect game animals than cattle. neutralizing antibody titres were high and were maintained for an extended period in buffalo, sable antelope and eland. the carrier state was ...19892584449
mixed function oxidase activity in the harbour seal (phoca vitulina) from sable is., n.s.one adult male, eight pups (including two full term foetuses) and nine adult female harbour seals (phoca vitulina) were analysed for indices of mixed function oxidase (mfo) activity. mfo activity was present in liver samples, but was at or below detection limits in samples of kidney, lung and pancreas. hepatic ethoxyresorufin o-de-ethylase and benzo[a]pyrene hydroxylase activities were similar to those reported in other seals and in other mammals. cytochromes p-450 and b5 concentrations were sli ...19862877778
molecular characterization of the drosophila vermilion locus and its suppressible alleles.we have cloned vermilion (v), one of the genes required for brown eye pigment synthesis in drosophila, using a mutant (vh2a) "tagged" with the transposon p factor. mutations that disrupt v gene expression are clustered within approximately 2 kilobases of dna. a 1.4-kilobase transcript, homologous to this same region, is present in v+ rna but absent in rna from several v mutants. the spontaneous v alleles that are suppressed by the suppressor of sable [su(s)] are apparently identical insertions o ...19863001735
molecular cloning of suppressor of sable, a drosophila melanogaster transposon-mediated suppressor.a hybrid dysgenesis-induced allele [su(s)w20] associated with a p-element insertion was used to clone sequences from the su(s) region of drosophila melanogaster by means of the transposon-tagging technique. cloned sequences were used to probe restriction enzyme-digested dnas from 22 other su(s) mutations. none of three x-ray-induced or six ethyl methanesulfonate-induced su(s) mutations possessed detectable variation. seven spontaneous, four hybrid dysgenesis-induced, and two dna transformation-i ...19863023894
reinforcers in infancy: classical conditioning using stroking or intra-oral infusions of milk as ucs.six-day-old rats received 20 forward pairings of an odor-conditioned stimulus (cs) with one of two unconditioned stimuli (ucs); 1) intra-oral milk infusions or 2) stroking with a sable-hair brush. these ucs's produce a common general response of increased behavioral activity, but different specific behaviors. for each ucs, additional pups received backward pairings of the cs and ucs, random pairings of the cs and ucs, cs only, ucs only, or no stimuli. four hours later, pups received a two-odor c ...19883371554
ascorbic acid concentrations in serum and urine of the grey seal (halichoerus grypus) on sable island. 19873654117
vitamin a status in the grey seal (halichoerus grypus) on sable island.vitamin a and beta-carotene concentrations in serum, liver and blubber fat were investigated in 65 grey seals of different age and sex in the pupping colony on sable island, canada to determine the vitamin a status. in none of the investigated samples could beta-carotene be detected. there were no differences in the concentration of vitamin a in serum, liver and blubber between male (213 micrograms/l, 376 micrograms/g, 22 micrograms/g respectively) and female (205 micrograms/l, 319 micrograms/g, ...19873679694
[structural adaptations of the articular cartilage in fur-bearing animals under conditions of mobility].adaptive properties of the articular cartilage and plasticity of its structures under various conditions of static-dynamic loading on joints have been studied when the data obtained in sables and minks, living in natural biocenosis and in cages, are compared. by means of optic, raster electron microscopy and microroentgenological analysis, peculiarities of structural formation of the articular cartilage have been revealed in ontogenesis, as well as succession in formation of its zonal architecto ...19863753231
gastrointestinal nematodes in captive roan and sable antelope. 19863798683
parelaphostrongylus tenuis in captive reindeer and sable antelope.parelaphostrongylus tenuis caused neurologic disease in 6 reindeer (rangifer tarandus tarandus) and 2 sable antelope (hippotragus niger) that were housed at the national zoological park conservation and research center in front royal, va. progressive hindlimb ataxia and weakness were seen in all affected animals. the parasite was recovered from the subdural space of one reindeer and was seen histologically within the neuropil of another reindeer and a sable antelope. in the other reindeer and sa ...19863957771
cryptosporidiosis in young artiodactyls.cryptosporidium was found in the intestinal tract of 10 blackbuck, 2 scimitar-horned oryx, 2 fringe-eared oryx, 2 addax, and 1 sable antelope that had diarrhea. cryptosporidia were most numerous in the small intestine, but also were found in the cecum, spiral colon, and colon. the small intestine had minimal inflammation in association with the cryptosporidia. salmonella typhimurium was isolated from 10 of the 51 animals evaluated, with extensive inflammation of the cecum, spiral colon, and colo ...19854077628
[etiology of fur-chewing in sables]. 19744450496
[reproductive function in female sables]. 19734784428
combined iris retractor and forceps and combined iris retractor and sable brush. 20075442020
[subcellular differentiation levels corresponding to diapause and embryo activation in the sable martes zibellina]. 19715557980
the triglycerides of sable fish (anaplopoma fimbria). ii. fatty acid distribution in triglyceride fractions as determined with pancreatic lipase. 19655845643
ancient oyster and bay scallop shells from sable island. 19676061802
basic haematological values in antelopes--ii. the hippotraginae and the tragelaphinae.basic haematological values in 49 animals of five species of the subfamily hippotraginae, namely the roan antelope hippotragus equinus, sable antelope hippotragus niger, adax antelope addax nasomaculatus, gemsbok oryx oryx gazella gazella and scimitar horned oryx oryx damah and in 51 individuals of five species in the subfamily tragelaphinae, including the bushbuck tragelaphus scriptus, nyala tragelaphus angasi, greater kudu tragelaphus strepsiceros, cape eland tautotragus oryx and bongo bocercu ...19846149054
[resistance to antibiotics and heavy metal salts of coagulase-positive staphylococci belonging to different biological types (ecovars)].a total of 189 strains of s. aureus isolated from cows, sheep, swines, poultry, monkeys, rabbits, foxes, and humans and 23 strains of s. intermedius isolated from minks and sables were studied. the staphylococci belonged to different biological types (according to the hajek-marsalek's scheme) and ecovars (according to the meyer-witte's scheme). the strains were studied with respect to their resistance to 10 antibiotics (benzylpenicillin, streptomycin, tetracycline, levomycetin, erythromycin, ole ...19826219614
detection and control of rotavirus infections in zoo animals.fecal specimens from 15 exotic animal species, with and without diarrhea, were examined for the presence of rotavirus, bacterial enteropathogens, and intestinal parasites. a commercial enzyme-linked immunosorbent assay was used to detect antigens of rotavirus. rotavirus was detected in the feces of 20 (57%) of 35 of the animals, which included addax (addax nasomaculatus), nyala (tragelaphus angasi), saiga (saiga tatarica), white-tailed gnu (connochaetus gnou), greater kudu (tragelaphus strepsice ...19836315660
[drug resistance of coagulase-negative staphylococci of animal origin depending on the source of the isolation and species classification of the strains].drug resistance of 271 strains of coagulase negative staphylococci isolated mainly from the upper respiratory tract and skin of healthy animals (cows, sheep, swine, hen, monkey, mink, sable and fox) was studied. benzylpenicillin, streptomycin, tetracycline, chloramphenicol, erythromycin, oleandomycin, neomycin, monomycin, kanamycin and novobiocin and heavy metal salts, such as mercurous chloride and cadmium sulfate were used in the study. unlike coagulase positive staphylococci isolated from the ...19846422841
an implant labeling technique employing sable hair probes as carriers for 3h-thymidine: applications to the study of facial morphogenesis.an implant labeling technique is described that utilizes sable hair probes as carriers for a tritiated thymidine marker. the protocol that was developed produced localized labeling of specific embryonic cell populations. this procedure was applied to the analysis of facial process development in chick embryos. evaluation of the technique demonstrated that the probe preparation procedure was consistently successful in producing labeled probes. using labeled probes, the procedure was reliable in p ...19846524694
[species characteristics of coagulase-negative staphylococci of animal origin].the specific identification of 271 strains of coagulase-negative staphylococci isolated from different animals (cows, sheep, swine, hens, monkeys, minks, sables, foxes, etc.) was carried out according to the scheme of akatov--devriese. species could be determined in 77.5% of the strains. the representatives of s. sciuri (55.5%) and s. xylosus (11.4%), very seldom occurring in humans, prevailed among the identified cultures. in 31.4% of coagulase-negative strains of animal origin the presence of ...19836637274
anaplasma infections in wild and domestic ruminants: a review.anaplasma marginale can be transmitted, will grow and can survive in a large number of domestic and wild animals. it is pathogenic in cattle, and usually produces nonapparent or mild infections in other species. anaplasma marginale has been recovered from cattle, sheep, goats, water buffalo (bubalus bubalis), white-tailed deer (odocoileus virginianus), mule deer (odocoileus hemionus hemionus), black-tailed deer (odocoileus hemionus columbianus), pronghorn (antilocapra americana americana), rocky ...19846716555
phase transition properties of aqueous dispersions of homologues of all-trans 2,3-dipalmitoylcyclopentano-1-phosphocholine.in a previous publication, (singer, m.a., jain, m.k., sable, h.z., pownall, h.h., mantulin, w.w., lister, m.d. and hancock, a.j. (1983) biochim. biophys. acta, 731, 373-377), we reported the properties of aqueous dispersions of the six diastereo-isomers of cyclopentanoid analogues of dipalmitoylphosphatidylcholine. two of these isomers displayed unusually high enthalpies of transition, about double that of dipalmitoylphosphatidylcholine. one of the high enthalpy isomers whose configuration is al ...19846743654
babesia, theileria and anaplasma spp. infecting sable antelope, hippotragus niger (harris, 1838), in southern africa.some observations are recorded on blood parasites of sable antelopes. blood smears of 124 of these antelopes from south africa and zimbabwe were examined and 7 were found to be positive for a babesia sp., identified as babesia irvinesmithi martinaglia 1936. a total of 70 of the smears were positive for theilerial piroplasms, while 1 smear had macroschizonts (with cytomeres) and microschizonts of a theileria (= cytauxzoon) sp. one blood smear was positive for an anaplasma sp. attempts to isolate ...19826817250
x-chromosome monosomy (37,xo) in a burmese cat with gonadal dysgenesis.a 37,xo-chromosome complement was detected in a 21/2-year-old sable burmese cat examined because of primary anestrus. the cat was smaller than its littermates; other somatic abnormalities associated with the xo karyotype in other species were not present. the ovaries, which did not respond to gonadotropin stimulation, contained inactive germinal epithelium (lacking follicles and primordial germ cells), which was similar to that of adult human patients with xo-gonadal dysgenesis.19836853322
indices of the endocrine function of the gonads at the initial stages of postnatal ontogenesis of female sable. 20177187008
[gonadal endocrine function indicators during initial stages of postnatal ontogenesis of the female sable].by means of radioimmunoassay, 17 beta-estradiol-, androgen- (testosterone and dihydrotestosterone) and progesterone contents were determined in the peripheral blood of the female sables, aged 2 to 27 months, during postnatal ontogenesis initial stage. under the action of seasonal factors steroid level was sharply decreased in the animals of the all ages within the autumnal period, whereas the gonadal function activation lowered over a period of february and march, when "the spring animation" of ...19817198788
[content of sex steroids in the blood of female sables in the initial stages of postnatal ontogenesis]. 19817261877
contaminants of fishes from great lakes-influenced sections and above dams of three michigan rivers: iii. implications for health of bald eagles.recently, there have been discussions of the relative merits of passage of fishes around hydroelectric dams on three rivers (au sable, manistee, and muskegon) in michigan. a hazard assessment was conducted to determine the potential for adverse effects on bald eagles that could consume such fishes from above and below dams on the three primary rivers. the hazard assessments were verified by comparing the reproductive productivities of eagles nesting in areas where they ate primarily fish from ei ...19957487154
[genetic variability of the sable (martes zibellinna l.) by blood protein genes].electrophoresis of blood proteins was used to determine, for the first time, the level of genetic variability of certain loci in the sable (martes zibellina l.,). variation of 23 blood proteins encoded by 25 genes was analyzed. polymorphism was revealed in six genes. the level of heterozygosity was estimated at 0.069; the proportion of polymorphic loci was 24%. data on the history of the sable population maintained at the farm, on geographical distribution of natural sable populations, and on th ...19957721064
relative immunocompetence of the newborn harbour seal, phoca vitulina.the immune system of many mammalian species is not fully developed at birth, with newborns obtaining temporary immunological protection from maternal antibodies. little is known of the immune system of the harbour seal, and developmental aspects of its immune system have not been systematically studied. we collected blood and milk samples from nine free-ranging mother-pup pairs throughout the lactation period on sable island, canada, in an effort to characterise developmental aspects of the immu ...19947810064
contaminants in fishes from great lakes-influenced sections and above dams of three michigan rivers. i: concentrations of organo chlorine insecticides, polychlorinated biphenyls, dioxin equivalents, and mercury.fishes of the great lakes contain hazardous chemicals such as synthetic halogenated hydrocarbons and metals. these fish can move from the lakes into the great lakes tributaries of michigan. in doing so, they transport concentrations of contaminants which may represent a risk to wildlife. concentrations of mercury (hg), total polychlorinated biphenyls (pcbs), 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (tcdd-eq), total ddt complex, aldrin, endrin, dieldrin, heptachlor, heptachlor epoxide, lin ...19948060164
contaminants in fishes from great lakes-influenced sections and above dams of three michigan rivers. ii: implications for health of mink.populations of mink (mustela vison) have declined in many areas of the world. such declines have been linked to exposures to synthetic, halogenated hydrocarbons. in the great lakes region, mink are fewer in areas along the shore of the great lakes and their tributaries where mink have access to fish from the great lakes. recently, there has been discussion of the relative merits of passage of fishes around hydroelectric dams on rivers in michigan. a hazard assessment was conducted to determine t ...19948060165
effects of energy mobilization during fasting and lactation on plasma metabolites in the grey seal (halichoerus grypus).1. the effects of fasting and lactation on plasma metabolites and insulin as well as morphometric parameters related to the mobilization of energy reserves were investigated in grey seals (halichoerus grypus) of different ages and sex during the 4 week terrestrial breeding period on sable island, ns, canada. 2. clear differences in body length and girth between the sexes were already recognizable in juveniles. fasting and lactation caused a marked decrease in adipose tissue thickness and girth i ...19938101163
the anatomy of the omasum of some zambian game species.the gross anatomy of the omasum of 11 different zambian game species are described. these include roan antelope, sable antelope, wildebeest, kudu, kafue lechwe, puku, reedbuck, impala, bushbuck, oribi and duiker. lamellar area and papilla density and shape are reported.19938129169
conservation of regulated alternative splicing and identification of functional domains in vertebrate homologs to the drosophila splicing regulator, suppressor-of-white-apricot.although several splicing regulatory proteins have been identified in drosophila through characterization of various genetic mutations, including sex-lethal, transformer, transformer-2, suppressor-of-white-apricot (su(wa)), and possibly suppressor-of-sable, none of these have been identified in vertebrates. we describe the cloning and characterization of human (hsswap) and mouse (mmswap) homologs of the su(wa) gene. comparison of the drosophila and mammalian proteins reveals five highly homologo ...19948206918
inhibition of oral carcinogenesis by glutathione.forty young adult syrian hamsters (mesocricetus auratus) were divided into four groups of 10 animals each. in group 1 (tumor control), the right buccal pouches were painted three times per week with a 0.5% solution of 7,12-dimethylbenz[a]anthracene (dmba) in heavy mineral oil (usp) with a no. 4 sable brush. in group 2 (experimental group), the right buccal pouches were painted with dmba, as in group 1. in addition, group 2 received 1 mg of reduced glutathione in 0.5 ml of mineral oil three times ...19938233979
evidence for a role of the drosophila melanogaster suppressor of sable gene in the pre-mrna splicing pathway.recessive mutations of the drosophila melanogaster suppressor of sable [su(s)] gene result in elevated accumulation of rna from vermilion (v) mutant alleles that have an insertion of the 7.5-kb retrotransposon 412 in the first exon of the v gene. during transcription of such a v mutant gene, the 412 sequences are incorporated into the primary transcripts and are subsequently removed by splicing at cryptic sites within 412 sequences. in a su(s)+ background, the level of these unusually spliced tr ...19948264653
the role of wild animals, other than buffalo, in the current epidemiology of foot-and-mouth disease in zimbabwe.between 1989 and 1992, 7970 wild ungulates, comprising 14 different species, were tested for antibodies to types sat 1, sat 2 and sat 3 foot-and-mouth disease (fmd) virus. of these 1.2% were found to be positive and these included impala (aepyceros melampus), eland (taurotragus oryx), waterbuck (kobus ellipsiprymnus) and sable (hippotragus niger). all the positive animals were either from the wildlife areas where buffalo (syncerus caffer) occur or from ranches where clinical fmd had occurred in ...19938270015
the r- and g-banded karyotypes of the sable antelope (hippotragus niger).the karyotype of the sable antelope has been investigated by rbg- and gtg-banding techniques. chromosome preparations were made from peripheral blood lymphocytes, and primary fibroblast cells were cultured from skin biopsies. the rbg-banded chromosomes were identified and classified according to the standard conventions used for cattle and goat. the diploid number of the sable antelope is 60. the autosomes are all acrocentric, the x is a large acrocentric, and the y is a minute acrocentric. comp ...19938270772
larval nematodes infecting amphiporeia virginiana (amphipoda: pontoporeioidea) on sable island, nova scotia.three species of larval nematodes were found infecting the nearshore subtidal amphipod amphiporeia virginiana collected from beaches on sable island, nova scotia. five and 2 sealworms, pseudoterranova decipiens s.l., were found in 2,020 and 2,044 amphipods, respectively, collected in 1991 and 1992, dissected, and examined microscopically. two sealworms, 1 paracuaria adunca and 1 ascarophis sp., were found in 5,157 amphipods collected in 1992 and enzymatically digested. infection with each specie ...19938277392
vitamin c enhances the development of carcinomas in the hamster buccal pouch experimental model.forty young adult male syrian hamsters (mesocricetus auratus) were divided into four groups of 10 animals. the animals in group 1 (tumor control) had the right buccal pouches painted three times a week with a 0.5% solution of 7,12 dimethylbenz(a)anthracene in heavy mineral oil usp with the use of a number 4 sable brush. the animals in group 2 (experimental group) had the right buccal pouches painted with the same solution as group 1. in addition, they received 1 mg ascorbic acid in 0.5 ml minera ...19938284076
longistrongylus curvispiculum (nematoda: trichostrongyloidea) in free-ranging exotic antelope in texas.longistrongylus curvispiculum was recovered from free ranging sable (hippotragus niger), addax (addax nasomaculatus) and biesa oryx (oryx beisa) on two ranches in central texas (usa). these nematodes represented a small portion of the total abomasal worm burden dominated by haemonchus contortus. female l. curvispiculum easily could be confused with other ostertaginae, but the spicules of the males were diagnostic.19938355363
immune function in free-ranging harbor seal (phoca vitulina) mothers and their pups during lactation.immune function in harbor seal mothers and their pups during lactation was studied on sable island, nova scotia, canada, during the springs of 1989 and 1990. methods included total white blood cell and differential counts, a protein a enzyme linked immunosorbent assay (elisa) for total immunoglobulin g (igg) quantification, and functionality testing of lymphocytes in vitro using the t-cell mitogen concanavalin a (cona). lymphocyte functionality and total igg levels were reduced in the mothers at ...19938445787
absence of geographic chromosomal variation in the roan and sable antelope and the cytogenetics of a naturally occurring hybrid.the determination of geographic chromosomal variation in rare or endangered species, or those of special management concern, is important, since geographically defined cytotypes can negatively influence breeding programs involving founders drawn from widely divergent localities. we cytogenetically analyzed specimens of the roan (hippotragus equinus) and sable antelope (h. niger) collected from widely divergent localities throughout their respective ranges. each species was characterized by a dip ...19958521725
multiple copies of mate elements support autonomous plasmid replication in aspergillus nidulans.the ama1 sequence is an efficient plasmid replicator and transformation enhancer in aspergillus nidulans. it comprises two long perfect inverted repeats (mate elements) flanking a short, unique, central spacer. subclone analysis indicates that the complete inverted duplication, but not the unique central spacer, is necessary for efficient plasmid replication. the smallest fragments able to affect transformation efficiency lie within the at-rich portions of the inverted repeats. we demonstrate th ...19968733240
stability, tissue metabolism, tissue distribution, and blood partition of da-1131, a new carbapenem.the stability of da-1131 after incubation in various ph solutions, human plasma, human urine, human gastric juice, rat liver homogenates and rat whole blood, the disappearance of da-1131 after incubation in the 9000g supernatant fraction of various rat tissue homogenates in the presence of nadph, the tissue distribution of da-1131 after intravenous (i.v.) administration of da-1131, 50 mg/kg, to rats, and the blood partition of da-1131 between plasma and blood cells from rabbit blood were studied ...19958746482
structure and expression of wild-type and suppressible alleles of the drosophila purple gene.viable mutant alleles of purple (pr), such as prbw, exhibit mutant eye colors. this reflects low 6-pyruvoyl tetrahydropterin (ptp) synthase activity required for pigment synthesis. ptp synthase is also required for synthesis of the enzyme cofactor biopterin; presumably this is why some pr alleles are lethal. the prbw eye color phenotype is suppressed by suppressor of sable [su(s)] mutations. the pr gene was cloned to explore the mechanism of this suppression. pr produces two ptp synthase mrnas: ...19968846895
use of electroejaculation to collect semen samples from wild seals.semen samples were obtained from 7 adult male gray seals (halichoerus grypus) at a colony on sable island, nova scotia, canada, using a portable electroejaculation system. males were anesthetized with tiletamine-zolazepam. a probe was inserted in the rectum, and a progressive series of 10 to 15 stimulations (1 to 9 v, 25 to 750 ma) was applied. males ejaculated after 15 to 29 minutes of stimulation and produced ejaculates with volumes of 4 to 17 ml that had vigorous, motile spermatozoa. although ...19968899030
[the structure of the skin of the external auditory canal and the antiviral activity of the cerumen in carnivorous mammals].the structure of the meatus acusticus externus skin was studied in the sable, mink, and european polecat with a parallel estimation of antiherpetic activity of the cerumen. morphologically, these species differ in the degree of fat cellulose development, vascularization, and association with the lymphoid tissue. lymphocytes are present both in the tissue and along the skin gland ducts and hair follicles up to the external surface only in the sable. the cerumen samples have antiviral activity als ...19968991526
a comparative ultrastructural study of the parotid gland acinar cells of nine wild ruminant species (mammalia, artiodactyla).the ultrastructural similarities and differences of the parotid gland acinar cells of nine wild ruminants (roe deer, nyala, tahr, eld's deer, red deer, pere david's deer, european mouflon, african buffalo, sable antelope) representing three feeding types i.e. concentrate selectors (cs), grass and roughage eaters (gr) and intermediate feeders (im) were compared. the parotid acinar cells of the cs contained more granular endoplasmic reticulum, golgi-complexes and secretory granules than those of t ...19969090994
the drosophila suppressor of sable protein binds to rna and associates with a subset of polytene chromosome bands.mutations of the drosophila melanogaster suppressor of sable [su(s)] gene, which encodes a 150-kda nuclear protein [su(s)], increase the accumulation of specific transcripts in a manner that is not well understood but that appears to involve pre-mrna processing. here, we report biochemical analysis of purified, recombinant su(s) [rsu(s)] expressed in baculovirus and in escherichia coli as maltose binding protein (mbp) fusions and immunocytochemical analysis of endogenous su(s). this work has sho ...19979121479
structure and amount of genetic variation at minisatellite loci within the subspecies complex of phoca vitulina (the harbour seal).the structure and amount of genetic variation within and between three subspecies of the harbour seal phoca vitulina was assessed by multilocus dna fingerprinting. bandsharing similarity indicates that the subspecies phoca vitulina richardsi (alaska, east pacific) is clearly separated from the other two subspecies, phoca vitulina concolor (sable island, west atlantic) and phoca vitulina vitulina (north sea, east atlantic). the subspecies also differ significantly in the estimated amount of heter ...19979172395
balancing foraging and reproduction in the male harbour seal, an aquatically mating pinnipedaquatically mating male harbour seals, phoca vitulinamust balance the competing demands of foraging and reproduction while at sea during the breeding season. time-depth recorders (tdrs) were attached to 31 adult male harbour seals to investigate changes in diving behaviour at sable island, nova scotia, during the 1992-1994 breeding seasons. male seals were captured, fitted with tdrs and weighed at the beginning of the season in late may, then recaptured for tdr removal and reweighing at the end ...19979299050
expiratory flow limitation in stable asthmatic patients during resting breathing.application of negative pressure at the mouth during tidal expiration (nep) provides a simple, rapid, noninvasive method for detecting expiratory flow limitation during spontaneous breathing. patients in whom nep elicits an increase in flow throughout expiration are not flow-limited (fl). in contrast, patients in whom application of nep does not elicit an increase in flow during most or part of tidal expiration are considered fl. we have used the nep technique to assess the prevalence of expirat ...19979309989
growth of cowdria ruminantium in tissue culture endothelial cell lines from wild african mammals.endothelial cell cultures were established from several wild african mammalian species. long-term cultures were established from three ruminants, stable antelope (hippotragus niger), buffalo (syncerus caffer), and eland (tragelaphus oryx), and from an omnivore, the bushpig (potamochoerus porcus). cowdria ruminanntium was isolated from plasma of clinically affected animals in these four cell lines and in bovine endothelial cells used routinely for c. ruminantium propagation. nineteen different st ...19989577776
male mating success in an aquatically mating pinniped, the harbour seal (phoca vitulina), assessed by microsatellite dna markers.similar to many other pinniped species, harbour seals (phoca vitulina) mate exclusively at sea. here we present the first attempt to measure male mating success in an aquatically mating pinniped. male mating success was estimated by paternity analysis in two cohorts of pups born at sable island, nova scotia, canada, using microsatellite dna markers. the genotypes of 275 pups born in 1994 and 1995 were compared to those of 90 candidate males at six microsatellite loci using a likelihood approach ...19989633104
critical role of arg433 in rat transketolase activity as probed by site-directed mutagenesis.it has been shown that one arginine per monomer at an unknown position is essential for enzyme activity of the homodimeric transketolase (tk) [kremer, egan and sable (1980) j. biol. chem. 255, 2405-2410]. to identify the critical arginine, four highly conserved arginine residues of rat tk (arg102, arg350, arg433 and arg506) were replaced with alanine by site-directed mutagenesis. wild-type and mutant tk proteins were produced in escherichia coli and characterized. the arg102-->ala mutant exhibit ...19989657977
the energetics of male reproduction in an aquatically mating pinniped, the harbour seal.the energy expenditure of breeding male harbour seals, phoca vitulina, on sable island, nova scotia, was investigated by measuring changes in body mass, body composition, and water flux using isotope dilution. seals lost 0.47% +/- 0.04% (n = 34) of their initial mass per day during the breeding season (4 wk), and fat, water, and protein accounted for 64.3% +/- 4.8%, 27.8% +/- 3.3%, and 6.9% +/- 1.4% of this mass loss, respectively (n = 31). total energy expenditure was estimated as 33.3 +/- 1.9 ...20069678499
isolation and characterization of a babesia species from rhipicephalus evertsi evertsi ticks picked off a sable antelope (hippotragus niger) which died of acute babesiosis.transmission of a babesia species to susceptible cattle by rhipicephalus evertsi evertsi ticks picked off a sable which died of acute babesiosis is described. the parasite was initially isolated by feeding r. e. evertsi ticks on a susceptible, splenectomised bovine which developed parasitaemia. blood stabilate from the parasitaemic bovine produced a fatal babesiosis in a spleen--intact bovine. clinical signs shown by the affected animals corresponded with those of acute babesiosis. parasitologic ...19989741050
helminths and bot fly larvae of wild ungulates on a game ranch in central province, zambia.helminths and bot fly larvae were collected from 11 wild ungulate species on a game ranch in the central province of zambia. new host-parasite records are: calicophoron sp. from defassa waterbuck kobus ellipsiprymnus defassa and kafue lechwe kobus leche kafuensis; avitellina centripunctata, gaigeria pachyscelis and gedoelstia cristata from tsessebe damaliscus lunatus lunatus; cooperia rotundispiculum from common reedbuck redunca arundinum; dictyocaulus filaria from greater kudu tragelaphus strep ...19989741058
characterization of estrous cyclicity in the sable antelope (hippotragus niger) through fecal progestagen monitoring.fecal progesterone metabolite monitoring techniques were validated for the sable antelope and used to characterize ovarian cycle dynamics and reproductive seasonality in a captive population at the national zoological park's conservation and research center. hormone was homogeneously distributed within fecal samples. longitudinal fluctuations in fecal progesterone metabolites were consistent with typical luteal phase patterns and corresponded closely with changes in serum progesterone. the lag t ...19989748411
spatial integration in infant sable antelope, hippotragus niger.i studied the relationship between the timing of birth relative to other births in the social group, and the rate of spatial integration into the herd in six cohorts of semi-free-ranging infant sable antelope at the national zoological park's conservation and research center near front royal, virginia. all calves spent the initial few days of life resting in seclusion ('hiding'), and interacted with mothers and other conspecifics only during activity bouts. with time, calves became increasingly ...19989790712
female distribution, genetic relatedness, and fostering behaviour in harbour seals, phoca vitulina.although harbour seals may not recognize their relatives, relatives could be chosen preferentially for fostering (i.e. kin selection) if harbour seals display natal philopatry coupled with breeding site fidelity, and thus kin are clustered within the colony. we used behavioural and genetic data to investigate population structure within the sable island breeding colony and to test whether harbour seals tend to foster related pups. adult females on sable island showed a high level of breeding-col ...199910049483
mitochondrial dna population structure of roan and sable antelope: implications for the translocation and conservation of the species.mitochondrial dna (mtdna) control region sequences were analysed to determine the geographical genetic structure of hippotragus niger (sable antelope) and h. equinus (roan antelope). analyses by amova, minimum evolution networks, parsimony, neighbour joining and maximum likelihood show that the roan antelope populations are geographically partitioned and that their phylogeography corresponds well with subspecific boundaries. in sharp contrast, however, our sequence data group all the sable antel ...199910065541
development and organization of ocular dominance bands in primary visual cortex of the sable ferret.thalamocortical afferents in the visual cortex of the adult sable ferret are segregated into eye-specific ocular dominance bands. the development of ocular dominance bands was studied by transneuronal labeling of the visual cortices of ferret kits between the ages of postnatal day 28 (p28) and p81 after intravitreous injections of either tritiated proline or wheat germ agglutinin-horseradish peroxidase. laminar specificity was evident in the youngest animals studied and was similar to that in th ...199910213088
prevalence of toxoplasma gondii antibodies in sera of domestic pigs and some wild game species from zimbabwe.serum samples of domestic pigs (sus scrofa), elands (taurotragus oryx), sable antelopes (hippotragus niger), warthogs (phacochoerus aethiopicus), bushpigs (koiropotamus [potamochoerus] koiropotamus), white rhinos (ceratotherium simus), african buffalos (syncerus caffer), wildebeest (connochaetas taurinus), and african elephants (loxodonta africana) from zimbabwe were tested for toxoplasma gondii igg antibodies by the modified agglutination test (mat) with whole formalized tachyzoites and mercapt ...199910219323
susceptibility and carrier status of impala, sable, and tsessebe for cowdria ruminantium infection (heartwater).three species of wild african ruminants, impala (aepyceros melampus), sable (hippotragus equinus), and tsessebe (damaliscus lunatus), were experimentally inoculated with in vitro culture-derived cowdria ruminantium organisms, the tick-borne causative agent of heartwater in domestic ruminants, to determine their susceptibility to infection. no clinical disease was observed in any of the ruminants. however, c. ruminantium was detected in the sable by the transmission of heartwater to susceptible s ...199910386439
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