xanthine oxidase/tyrosinase inhibiting, antioxidant, and antifungal oxindole alkaloids from isatis costata.phytochemical investigations on the ethyl acetate soluble fraction of the whole plant of isatis costata linn. (brassicaseae) led to the isolation of the oxindole alkaloids costinones a (1), b (2), isatinones a (3), b (4), indirubin (5), and trisindoline (6). compounds 1-6 displayed significant to moderate inhibition against xanthine oxidase enzyme with ic50 values ranging from 90.3+/-0.06 to 179.6+/-0.04 microm, whereas the standard inhibitor of xanthine oxidase (allopurinol) had an ic(50) value ...201020645747
quantitative real-time pcr assay for rapid detection of plant and human pathogenic macrophomina phaseolina from field and environmental samples.a real-time qpcr assay was developed to detect and quantify macrophomina phaseolina abundance in rhizosphere soil and plant tissue. both taqman and sybr green techniques were targeted on ~ 1 kb sequence characterized amplified region (scar) of m. phaseolina and two sets of specific primers were designed for sybr green (mpsyk) and taqman (mptqk) assays. no cross-hybridization and no fluorescent signal exceeding the baseline threshold was observed in taqman and sybr green assays, respectively. the ...201021186328
suppression of charcoal rot of chickpea by fluorescent pseudomonas under saline stress condition.the ability of fluorescent pseudomonas strain eki, in production of biocontrol and plant growth promotory (pgp) metabolites under saline stress was evaluated. strain eki could tolerate nacl up to 1,550 mm and showed biocontrol of macrophomina phaseolina (76.19%) in the presence of up to 400 mm nacl. strain eki was able to produce iaa, siderophore and pyocyanin with gradual reduction of up to 76.31, 45.46, and 48.99%, respectively, as nacl concentration increased from 0 to 500 mm. reduced growth ...201121331555
metabolites of the fungistatic agent 2+¦-methoxyclovan-9+¦-ol by macrophomina phaseolina.biotransformation of 2+¦-methoxyclovan-9+¦-ol (1), a fungistatic agent against botrytis cinerea, was investigated with macrophomina phaseolina. demethoxylation, regioselective oxidation at c-9 and c-13, and inversion of the configuration at c-9 of compound 1 afforded six oxidative metabolites, 2+¦-methoxyclovan-9-one (2), clovan-2+¦,9+¦-diol (3), clovan-2+¦,9+¦-diol (4), clovan-2+¦,13-diol-9-one (5), 2+¦-methoxyclovan-9+¦,13-diol (6), and clovan-2+¦,9+¦,13-triol (7). compounds 5-7 are described ...201121391597
opportunistic invasive fungal pathogen macrophomina phaseolina prognosis from immunocompromised humans to potential mitogenic rbl with an exceptional and novel antitumor and cytotoxic effect.with the ever-increasing risk for fungal infections, one can no longer ignore fungi. it is imperative that clinical manifestations "presume fungus" with their epidemiologic and pathogenic features when evaluating a potentially infected patient. in the high-risk patient groups, fungi with intrinsic resistance to antifungal agents already exist, with a tendency to emerge as opportunistic pathogens. one of the smart pathogens is macrophomina phaseolina, with the potential to disarm plant, animal, a ...201121553299
microsatellites from the charcoal rot fungus (macrophomina phaseolina).microsatellite loci were identified from the charcoal rot fungus (macrophomina phaseolina). primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic dna from 55 fungal isolates collected predominantly from two soybean fields in mississippi. twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. these microsatellites will be useful in population and pathogenicity studies to correspond with development of po ...200921564800
evaluation of coumarin derivatives as anti-fungal agents against soil-borne fungal pathogens.development of new and safer pesticides that are target-specific is backed by a strong federal, public and commercial mandate. in order to generate a new generation of pesticides that are more ecologically friendly and safe, natural products are being evaluated for pesticidal activities. many plant-derived chemicals have proven pesticidal properties, including compounds like sesamol (3,4-methylenedioxyphenol), a lipid from sesame oil and coumarins (1,2-benzopyrone) found in a variety of plants s ...200718396811
the synthetic strigolactone gr24 influences the growth pattern of phytopathogenic fungi.strigolactones that are released by plant roots to the rhizosphere are involved in both plant symbiosis with arbuscular mycorrhizal fungi and in plant infection by root parasitic plants. in this paper, we describe the response of various phytopathogenic fungi to the synthetic strigolactone gr24. when gr24 was embedded in the growth medium, it inhibited the growth of the root pathogens fusarium oxysporum f. sp. melonis, fusarium solani f. sp. mango, sclerotinia sclerotiorum and macrophomina phase ...201121688170
xyloglucan endotransglycosylase/hydrolase genes from a susceptible and resistant jute species show opposite expression pattern following macrophomina phaseolina infection.two of the most widely and intensively cultivated jute species, corchorus capsularis and corchorus olitorius, suffer severely from a stem rot disease caused by the fungus macrophomina phaseolina. wild jute species, c. trilocularis, shows resistance to this pathogenic fungus. in this study, the technique of differential display was applied to identify genes which are differentially expressed, under both infected and un-infected conditions, between c. trilocularis and c. olitorius var o-72. two xy ...201223336031
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