Publications

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experimental transovarial transmission of kyasanur forest disease virus in haemaphysalis spinigera. 196314058628
notes on african haemaphysalis ticks. v. redescription and relationships of h. silacea robinson, 1912, from south africa (ixodoidea, ixodidae). 196314070480
redescription of cotypes and all stages of haemaphysalis intermedia warburton and nuttall, 1909 (=h. parva neumann, 1908, preoccupied) from ceylon and india (ixodoidea, ixodidae). 196314070482
[research on "tick paralysis" observed in chickens in bulgaria and caused by nymphs of haemaphysalis punctata. can. and franz]. 196314074042
[on spontaneous infection of haemaphysalis japonica douglasi nutt. and warb. ticks by d. sibiricus rickettsia in the primorsk region]. 196314151783
[on several palearctic haemaphysalis species (acarina, ixodoidea)]. 196314152060
tick-borne encephalitis in the ussr.the author gives the history of the study of tick-borne encephalitis in the ussr, following it up with a description of the etiological and epidemiological aspects and of the pathology, symptomology, diagnosis, treatment, and prevention of the disease. he stresses that other ticks as well as the main vectors of the virus, ixodes, transmit the disease, in particular dermacentor and haemaphysalis. a detailed account of tick-borne encephalitis in the ussr may be of value in the study of the rather ...196414153408
ticks (ixodidae) on migrating birds in egypt, spring and fall 1962.over a number of years studies have been carried out in egypt on the transport by migrating birds of ticks that may transmit pathogens of man and animals. in continuation of these investigations 11 036 birds migrating southwards through egypt were examined for ticks during the fall of 1962. the 881 infested birds (comprising 24 species and sub-species represented by 10 612 individuals) yielded 1442 ticks. tick-host relationships were similar to those of previous years except that in 1962 the pre ...196414163959
description of immature stages of haemaphysalis howletti (ixodoidea: ixodidae) and redescription of adults. 196414169544
haemaphysalis kyasanurensis sp. n., a member of the formosensis group in southern india and ceylon (ixodoidea, ixodidae). 196414170770
haemaphysalis cornigera shimoga subsp. n. from southern india (ixodoidea, ixodidae). 196414170771
transmission of kyasanur forest disease virus by haemaphysalis turturis, haemaphysalis papuana kinneari and haemaphysalis minuta. 196414184087
studies on southeast asian haemaphysalis ticks (ixodoidea, ixodidae). redescription, hosts, and distribution of h. traguli oudemans. the larva and nymph of h. vidua w. and n. identity of h. papuana toxopei warburton (new combination). 196414244810
haemaphysalis nepalensis hoogstraal, 1962 (ixodoidea: ixodidae), systematic position based on description of the nymph, and the host and locality records. 196414244811
notes on african haemaphysalis ticks. vi. h. spinulosa neumann, and its relation to biological and nomenclatorial problems in the h. leachii group of africa and asia (ixodoidea, ixodidae). 196414244812
studies on southeast asian haemaphysalis ticks (ixodoidea, ixodidae). h. atherurus sp. n. and redescription of type material of h. birmaniae supino, 1897. 196514259471
hymenopterous parasites from an adult haemaphysalis leachii leachii (acarina: ixodidae). 196514275219
hosts and distribution of haemaphysalis kashmirensis with descriptions of immature stages and definition of the subgenus herpetobia canestrini (resurrected). 196514339387
records and new synonymy of new world haemaphysalis ticks, with descriptions of the nymph and larva of h. juxtakochi cooley. 196014410554
experimental transmission of an unnamed bovine babesia by hyalomma spp., haemaphysalis longicornis and boophilus microplus.experiments were undertaken to determine the mode of transmission to cattle of an unnamed babesia sp. by hyalomma anatolicum anatolicum, hyalomma detritum, hy. rufipes koch, haemaphysalis longicornis and boophilus microplus. the unnamed babesia species designated babesia u sp. was isolated by infesting cattle with nymphs from female hy. a. anatolicum ticks collected from xinjiang province. adults of laboratory reared hy. a. anatolicum, hy. detritum and hy. rufipes koch were infected with babesia ...200314519316
[spontaneous infection of ixodes ticks with salmonella].an epizootological study of the ticks gathered from the cattle and ground surface in the dedoplistskaroisky and dushetsky districts of eastern georgia has identified 5 salmonella a typhimurium strains. the strains were isolated from the ticks haemaphysalis punctata, rhipicephalus bursa, hyalomma plumbeum. they were found to be resistant to 10 of 16 antibiotics. the strains showed a high sensitivity only to 3 antibiotics, such as gentamicin, cirpofloxacin, and nitrofurantoin and 3 strains were hi ...200314564841
the argasidae, ixodidae and nuttalliellidae (acari: ixodida): a world list of valid tick names.the world's argasid tick fauna comprises 183 species in four genera, namely argas, carios, ornithodoros and otobius in the family argasidae. the ixodid tick fauna consists of 241 species in the genus ixodes and 442 species in the genera amblyomma, anomalohimalaya, bothriocroton, cosmiomma, dermacentor, haemaphysalis, hyalomma, margaropus, nosomma, rhipicentor and rhipicephalus in the family ixodidae, with the genus boophilus becoming a subgenus of the genus rhipicephalus. the family nuttallielli ...200214570115
preliminary observations on specific adaptations of exophilic ixodid ticks to forests or open country habitats.some features of three-host exophilic ticks important for their survival differ at the generic level in relation to the types of their habitat. ixodes and haemaphysalis are typical forest genera. hyalomma is an open country genus, whereas dermacentor and rhipicephalus, having some features of forest ticks, are closer to the ticks of open country. forest ticks encounter rather stable and favorable temperature, humidity and illumination conditions as compared with open country ticks. a few differe ...200214570125
detection and identification of spotted fever group rickettsiae in ticks collected in southern croatia.a total of 197 ticks belonging to four species (haemaphysalis punctata, hyalomma marginatum, rhipicephalus bursa and dermacentor marginatus) collected in october 2000 from domestic animals in southern croatia were examined for the presence of rickettsiae by molecular techniques. specific sequences of the rickettsiae were detected in 25 (12.7%) of ticks tested. the prevalence of infection in d. marginatus and h. marginatum ticks was 36.8 and 64.7%, respectively. none of the ticks belonging to the ...200214570128
phylogenetic analysis of theileria species transmitted by haemaphysalis qinghaiensis.the phylogenetic relationships between six isolates of theileria spp. infective to small ruminants, and two isolates of theileria spp. infective to yak, all transmitted by haemaphysalis qinghaiensis, together with the theileria orientalis/sergenti/buffeli group and t. sinensis, were analyzed using the 18s ssrrna gene sequence. the target dna segment was amplified by polymerase chain reaction (pcr). the pcr product was used either for direct sequencing or was ligated to the pcr ii vector for sequ ...200414598167
parasites of domestic and wild animals in south africa. xliii. ixodid ticks of domestic dogs and cats in the western cape province.ticks were collected at monthly intervals for 16 consecutive months from individual dogs by their owners in or close to the town of stellenbosch, western cape province. they were also collected for 27 consecutive months from dogs presented for a variety of reasons at three veterinary clinics in stellenbosch, and from dogs upon admission to an animal welfare shelter. at one of the veterinary clinics ticks were also collected from cats. dog owners collected six ixodid species from their pets and t ...200314621314
first evidence of anaplasma platys in rhipicephalus sanguineus (acari: ixodida) collected from dogs in africa.a total of 27 ticks, comprising rhipicephalus sanguineus (latreille) (n = 21), haemaphysalis leachi (andouin) (n = 4) and haemaphysalis paraleachi (camicas, hoogstraal & el kammah) (n = 2) were recovered from two clinically healthy female dogs in the democratic republic of the congo. dna of anaplasma platys was detected in a female r. sanguineus, using primers derived from the 16s rrna gene, which amplify members of the family anaplasmataceae . anaplasma platys dna was also detected in the blood ...200314621316
phylogenetic analysis of spotted fever group rickettsiae based on glta, 17-kda, and rompa genes amplified by nested pcr from ticks in japan.in order to understand the natural situation of rickettsiae in the ticks in japan, the rickettsial genes, glta gene, rompa gene, and 17-kda gene, were amplified from the ticks by nested pcr. the prevalences of rickettsial glta genes among haemaphysalis formosensis, h. longicornis, h. megaspinosa, ixodes ovatus, h. flava, h. kitaokai, and i. persulcatus were 62, 57, 24, 24, 19, 13, and 10%, respectively; 26% (186/722) being the average. the glta genes amplified from the ticks were classified into ...200314638993
host-seeking activity of ixodid ticks in relation to weather variables.ixodid ticks were monitored in a temperate deciduous broad-leaved forest in south moravia (czech republic). relative abundance of the ticks collected before noon (10.00-12.00 h) was compared to several weather variables (air and soil temperatures, relative humidity, precipitation, wind speed, and derived values) using the pearson correlation coefficient. the tick numbers were found to be most closely related to the amplitude of the soil (-5 cm) temperature between 07 h and 14 h (tsamp, in ixodes ...200314714663
ability of ixodes persulcatus, haemaphysalis concinna and dermacentor silvarum ticks to acquire and transstadially transmit borrelia garinii.in china, the ability of ixodes persulcatus, haemaphysalis concinna and dermacentor silvarum to transmit borrelia spirochetes was determined under laboratory conditions. results showed that all three tick species can acquire spirochetes by feeding on infected mice. however, the capability of the three species to maintain spirochetes was different. only i. persulcatus is able to maintain spirochetes alive during molting, and subsequent tick stages transmitted the infection to naive mice. in h. co ...200314756409
[a new type of spotted fever group rickttsiaes detected in the area of changbai mountain, jilin province].in order to find out the current situation of tick-borne spotted fever in the area of changbai mountain, jilin province.200314761631
prevalence of ixodid ticks in nilgiri district of tamil nadu state (india).the nilgiri hills provides favourable ecological conditions for the propagation of haematophagous arthropods due to its richness in vegetation and animal fauna. a study was undertaken by the nicd during august to november 1996 on the prevalence of ectoparasitic ticks from different localities of the nilgiris district of tamil nadu state. the ticks were hand picked from various domestic animals of the study area and identified. a total of 1232 adults and immatures of ticks were collected from dom ...200214768830
isolation and characterization of rocky mountain spotted fever rickettsiae from the rabbit tick haemaphysalis leporis-palustris packard. 195114816519
identification of the coxiella sp. detected from haemaphysalis longicornis ticks in korea.two haemaphysalis longicornis ticks were found positive in pcr assay of com-1 gene to detect coxiella burnetii dna from 100 ticks. the nucleotide sequences of com-1 and 16s rrna gene were determined from 2 ticks and compared with those of other c. burnetii strains. the results suggest that h. longicornis harbor coxiella sp. bacteria in korea. furthermore, icd, cbhe', and cbbe' genes are c. burnetii specific genes whereas com-1 gene is coxiella genus specific gene. this study gives the first docu ...200414978338
seasonal activity of ticks on small ruminants in tamil nadu state, india.the seasonal activity of ticks on sheep and goat populations was examined from december 2000 to november 2001 in the state of tamil nadu, india. this longitudinal study spread over four seasons and seven agroclimatic zones took into consideration the spectrum of species involved, the levels of infestations, the seasonal epidemiology of ticks and the associated management practices. the most common species of tick spread throughout the state was haemaphysalis bispinosa followed by rhipicephalus h ...200414998311
detection of ehrlichial dna in haemaphysalis ticks recovered from dogs in japan that is closely related to a novel ehrlichia sp. found in cattle ticks from tibet, thailand, and africa.tick dna samples from dogs in japan were examined for ehrlichia infection by 16s rrna gene-based pcr and sequencing. three positive samples were detected from haemaphysalis ticks, and higher levels of similarity (98.46 to 99.06%) were found to recently detected ehrlichia spp. from cattle ticks in tibet, thailand, and africa.200415004117
ectoparasites of the pallas squirrel, callosciurus erythraeus, introduced to japan.the squirrel callosciurus erythraeus (pallas) (rodentia: sciuridae) was intentionally introduced to japan in 1935 and has become established throughout much of the country. although they live mainly in forests, pallas squirrels come into gardens and are frequently fed by people or kept as pets, so their ectoparasites could be of potential medical as well as veterinary importance. during 2001-2003 we conducted the first ectoparasite survey of pallas squirrels in japan. from 105 c. erythraeus capt ...200415009447
cloning and sequencing of putative calreticulin complementary dnas from four hard tick species.calreticulin (crt) is a calcium-binding protein and has many functions in eukaryotic cells. crt is possibly involved in parasite host immune system evasion. to better understand the molecular basis of crt in ticks, we cloned and sequenced 4 full-length complementary dnas (cdnas) from the hard tick species, dermacentor variabilis, haemaphysalis longicornis, ixodes scapularis, and rhipicephalus sanguineus, using the technique of rapid amplification of cdna ends. the deduced amino acid sequences sh ...200415040669
[dichotomous identification keys of ticks (acari: ixodidae), livestock parasites in north africa].the known fauna of livestock in the maghreb consists of 5 established species. five other species recorded seem to be introduced by imported animals. in this paper, dichotomous keys are given for species and subspecies of the genus hyalomma. rhipicephalus and haemaphysalis parasite of livestock in north africa. for the ticks of the other genera which are represented by only one species, we provide an identification description. notes on geographical distribution, host and period of activity are ...200215072244
estimation of the density of nymphs of the bush tick, haemaphysalis longicornis (acari: ixodidae), by the catch effort method.the density of nymphs of the bush tick, haemaphysalis longicornis, was investigated by the catch effort method with flagging. the spatial distribution of h. longicornis nymphs fit the model of contagiously distributed colonies by iwao's m*-m analysis (iwao 1968). a sequential sampling method was used to predict the theoretical point at which to stop sampling. our analysis showed that five quadrats (4 m x 4 m) were sufficient to estimate the density of h. longicornis nymphs with a mean density of ...200415139273
the mitochondrial genomes of soft ticks have an arrangement of genes that has remained unchanged for over 400 million years.there are two major groups of ticks: soft ticks and hard ticks. the hard ticks comprise the prostriate ticks and the metastriate ticks. the mitochondrial (mt) genomes of one species of prostriate tick and two species of metastriate ticks had been sequenced prior to our study. the prostriate tick has the ancestral arrangement of mt genes of arthropods, whereas the two metastriate ticks have rearrangements of eight genes and duplicate control regions. however, the arrangement of genes in the mt ge ...200415157222
cloning, expression and partial characterization of a haemaphysalis longicornis and a rhipicephalus appendiculatus glutathione s-transferase.the ticks haemaphysalis longicornis and rhipicephalus appendiculatus are important parasites worldwide. the current method for control of cattle ticks involves the use of chemicals. nevertheless, parasite resistance is an ever increasing global problem. glutathione s-transferases (gsts) play a central role in detoxication of xenobiotic and endogenous compounds. several authors have noted that an increase in gst activity is associated with resistance to insecticides and acaricides. in the present ...200415157234
questing height of nymphs of the bush tick, haemaphysalis longicornis, and its closely related species, h. mageshimaensis: correlation with body size of the host.the questing height (i.e. ambush height) of ticks on a plant plays an important role in host selection. to test the hypothesis that the questing height of ticks in a locality had adapted to the body size of the host in that locality, we examined the questing height of nymphs of the ticks, haemaphysalis longicornis and h. mageshimaensis, at 7 locations in japan. sika deer, cervus nippon, is the primary host of these ticks and there is considerable geographical variation in the body size of sika d ...200415180318
a laboratory comparison of the life cycles of the dog ticks haemaphysalis leachi and rhipicephalus sanguineus.engorged female haemaphysalis leachi and rhipicephalus sanguineus as well as their eggs, flat and engorged larvae and nymphs were incubated at combinations of five temperatures and three relative humidities. mean pre-oviposition periods for h. leachi varied between 4.3 days and 12.1 days and for r. sanguineus between 4.8 days and 21.0 days. haemaphysalis leachi converted up to 65.7% of their body mass into eggs and produced up to 16.3 eggs/mg body mass, with 4801 eggs the maximum number deposite ...200415185571
[detection of rickettsia hulinii in ticks of the haemaphysalis concinna species in russia].the presence of rickettsia hulinii was detected in ticks of the haemaphysalis concinna species in the asiatic part of russia. it was the first detection of these rickettsiae outside the territory of the chinese people's republic. this tick is spread over a broken area and may occur in different regions of eurasia. at present at least 3 rickettsial species of the tick-borne spotted fever group were found in ixodes ticks on the territory of eastern siberia: r. sibirica, known to be the etiological ...200415188554
species composition, distribution, and ecological preferences of the ticks of grazing sheep in north-central spain.the distribution and ecological preferences of tick (acari: ixodidae) parasites of grazing sheep in the region of aragón (north-central spain) were surveyed on flocks four times a year and mapped into a 5 x 5 km grid. nine tick species were found. these were species of the rhipicephalus sanguineus group (about 95% of them rhipicephalus turanicus pomerantsev, in 91% of cells of the grid), rhipicephalus bursa canestrini & fanzago (79% of cells), dermacentor marginatus (sulzer) (58% of cells), haem ...200415189237
an updated list of the ticks of ghana and an assessment of the distribution of the ticks of ghanaian wild mammals in different vegetation zones.twenty one species of ticks belonging to five genera of the family ixodidae (order acari, sub-order ixodida) - amblyomma, haemaphysalis, hyalomma, ixodes and rhipicephalus (including the sub-genus rhipicephalus (boophilus)) - were collected from 1260 mammals, representing 29 species, 14 families and 6 orders, in four vegetation zones in ghana during the period 1971-1978. four other species were collected from humans in 1977. in all, eight species appeared to be new records for ghana: amblyomma t ...200415191626
[new evidence for the detection of ehrlichia and anaplasma in ixodes ticks in russia and kazakhstan].polymerase chain reaction and sequence analysis were used to screen ehrlichia and anaplasma dna in 900 specimens of ixodes ticks of four genera (dermacetor, haemaphysalis, ixodes, and hyalomma) collected on 14 administrative territories of russia and kazakhstan. anaplasma phagocytophilia dna was detected and identified in ixodes persulcatus ticks gathered in the altai and primorye territories. ehrlichia muris dna was genotyped in the i. persulcatus ticks collected in the tyumen, omsk, and novosi ...200415193042
in vivo immunomodulatory effects of ixodid ticks on ovine circulating t- and b-lymphocytes.selected aspects of the ovine immune system were examined during the course of repeated infestations with the ixodid ticks, haemaphysalis bispinosa and hyalomma anatolicum anatolicum that naturally infest sheep, either individually or together. by the use of flow cytometry it was shown that total t-lymphocyte numbers were significantly reduced from the sixth through the ninth days of all infestations. gamma/delta (gammadelta+) and cd8+ t-lymphocytes were significantly depleted during tick feedin ...200415225295
cloning and molecular characterization of a cubilin-related serine proteinase from the hard tick haemaphysalis longicornis.serine proteinases are one of the largest proteolytic families of enzymes, and have diverse cellular activities in mammalian tissues. we report here the cloning and molecular characterization of a cdna encoding the serine proteinase of the hard tick haemaphysalis longicornis (hlsp). the hlsp cdna is 1570 bp long and the deduced precursor protein consists of 464 amino acids with a predicted molecular mass of 50.4 kda and a pi of 8.2. the preprotein, consisting of 443 amino acids, was predicted to ...200415262284
ticks (acari: ixodidae) parasitizing wild carnivores in phu khieo wildlife sanctuary, thailand.ixodid ticks were collected and identified from 8 wild carnivore species in phu khieo wildlife sanctuary, northeastern thailand. six tick species belonging to 4 genera were recovered and identified from 132 individuals. these included amblyomma testudinarium (n = 36), haemaphysalis asiatica (n = 58), h. hystricis (n = 31), h. semermis (n = 3), rhipicephalus haemaphysaloides (n = 3), and ixodes granulatus (n = 1). leopard cats (prionailurus bengalensis) (n = 19) were infested with 4 tick species, ...200415270119
regeneration of haller's sensory organ in two species of hard ticks of the genus haemaphysalis (acari: ixodidae).a study of haller's organ regeneration in nymphs and adults of haemaphysalis turturis and parthenogenetic females of h. longicornis, from which the forelegs had been amputated during the previous instar, revealed structural changes in regenerated organs. the adult regenerates reestablished atavistic structural features, while the nymphal regenerates retained larval features, which is typical of regenerates of two other genera examined previously (ixodes and hyalomma). data on regeneration of hal ...200415285145
cross-sectional survey of ticks (acari: ixodidae) in sheep from an area of the southern italian apennines.a cross-sectional survey of ticks was conducted on 197 ovine farms with animals pasturing in an area (3971 km2) of the southern italian apennines. the farms were selected to be uniformly distributed throughout the study area using geographical information system (gis). ticks were collected from 309 (31.4%) out of the 985 sheep sampled, belonging to 92 (46.7%) out of the 197 farms included in the study. the following tick species were found (farm prevalence): dermacentor marginatus (37.6%), haema ...200415285146
[effect of of methoprene and retinoic acid on characteristics of haller's organ regeneration in the tick haemaphysalis longicornis (acari, ixodidae)].the results of experiments on regeneration of the haller's sensory organ in the metastriate ixodid tick haemaphysalis longicornis neumann in the presence of the juvenoid methoprene and retinoic acid and of studies of its structural changes by sem confirmed the similarity of prostriate and metastriate ixodids as concerns the juvenalizing effect of the above morphogens on regenerative processes during nymphal-imaginal metamorphosis. however, the metastriate ticks (haemaphysalis and earlier studied ...200415334824
enzymatic characterization of a cubilin-related serine proteinase from the hard tick haemaphysalis longicornis.in the present study, we performed enzymatic characterization of haemaphysalis longicornis serine proteinase (hlsp) with a view to shed light on the mechanisms of blood digestion in the hard ticks. escherichia coli-expressed recombinant hlsp (rhlsp) was shown to potently hydrolyze the synthetic substrates bz-(dl)-arg-pna, z-ala-ala-leu-pna and suc-ala-ala-ala-pna and yielded an activity of 31.5, 88.2 and 18.3 mumol/min/mg protein, respectively at an optimum temperature of 25 degrees c. however, ...200415528848
[genetic identification of rickettsiae of the tick-borne spotted fever group, isolated in the foci of tick-borne rickettsiosis].a total of 25 rickettsial cultures of the tick-borne spotted fever (tbsf) group from the collection of the research institute of infections in omsk, isolated from different sources in the territory of the russian federation (from the urals to the far east) during the period of 1954-2001) were studied by the methods of genetic analysis. the fragments of the gene coding the outer-membrane protein of 190 kd (ompa) and synthetase citrate (glta) of the rickettsiae under the study were sequenced. 23 i ...200415554312
gene silencing of a cubilin-related serine proteinase from the hard tick haemaphysalis longicornis by rna interference.rna interference (rnai) has been recently exploited to determine gene function by degrading specific mrnas in several eukaryotic organisms. we constructed a double stranded rna (dsrna) from a previously cloned haemaphysalis longicornis serine proteinase (hlsp) gene to test the importance of the function of the hlsp gene product during blood-feeding. growth of unfed ticks treated with hlsp dsrna was significantly inhibited compared to that of pbs-treated ticks. this inhibition was supported by th ...200415585970
[random amplified polymorphic dna analysis of the genomes among 7 species of ticks].to study genomic polymorphic dna and genetic distance of 7 species of ticks.200415587156
immunization effect of recombinant p27/30 protein expressed in escherichia coli against the hard tick haemaphysalis longicornis (acari: ixodidae) in rabbits.we investigated the induction of resistance to haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant h. longicornis p27/30 protein. the success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. previously, we cloned a gene encoding 27 kda and 30 kda proteins (p27/30) of h. longicornis, and identified p27/30 as a troponin i-like protein. in this study, rabbits that were immunized with recombinant p2 ...200415591837
a comparison of small-subunit ribosomal rna gene sequences of bovine babesia species transmitted by haemaphysalis spp. in china.the ribosomal small-subunit rna gene sequences of six chinese babesia stocks infective to cattle, including a babesia bigemina isolate, a b. bovis isolate, two b. ovata isolates, a babesia sp. wenchuan isolate and a b. major isolate, were compared and analyzed. the target dna segment was amplified by polymerase chain reaction and the product ligated into the pgem-t easy vector for sequencing. the length of the 18s rrna gene of all babesia species involved in this study varied between 1,653 and 1 ...200415609061
lyme disease and babesiosis: preliminary findings on the transmission risk in highly frequented areas of the monti sibillini national park (central italy).the monti sibillini national park is a recently borne protected area in the central apennines that has become an important tourist destination. mountain grasslands and woods have made it also a suitable habitat for sheep and bovine cattle, as well as for wild animals such as the wild bore and the roe deer. therefore, a preliminary investigation was conducted to assess the risk of transmission of tick-borne zoonoses, such as lyme disease and babesiosis, by actively looking for both the arthropod ...200415631066
molecular cloning and sequence analysis of cdnas encoding for boophilus microplus, haemaphysalis longicornis and rhipicephalus appendiculatus actins.the nucleotide and deduced amino acid sequences of the actins from ticks, boophilus microplus, haemaphysalis longicornis and rhipicephalus appendiculatus, have been determined. nucleotide sequence analysis showed open reading frames of 1128-nucleotide-long encoding proteins of 376 amino acids with a predicted molecular weight of 41.82 kda each. comparison between the nucleic acid and deduced amino acid sequences as well as structural and phylogenetic analyses of these genes confirmed the high si ...200415631908
detection of a spotted fever group rickettsia in the tick haemaphysalis juxtakochi in rondonia, brazil.the tick genus haemaphysalis is represented by four species in the new world, of which only the species haemaphysalis leporispalustris has been associated with rickettsiae. the present study reports for the first time the presence of a rickettsia strain in the tick haemaphysalis juxtakochi. a free-living male of h. juxtakochi, collected in the state of rondonia, western amazon, brazil, was subjected to dna extraction and tested by pcr targeting the four rickettsial genes: glta, 17-kda, ompa and ...200415631911
a serine protease inhibitor (serpin) from haemaphysalis longicornis as an anti-tick vaccine.the application of anti-tick vaccine has been shown to be the most promising alternative tick control strategy compared to the current use of acaricides that suffer from a number of limitations. the success of this strategy is dependent on the cloning, and characterization of tick molecules involved in the mediation of tick central physiological roles. rapid amplification of the cdna ends (race) and primers designed based on a conserved serpin amino acid motif (navyfkg) were used to clone a cdna ...200515652673
molecular differentiation of metastriate tick immatures.hard ticks, family ixodidae, are divided into two groups, the metastriata and the prostriata, based on morphological differences. in the united states, there are four medically important genera of the ixodidae: ixodes, amblyomma, dermacentor, and rhipicephalus. ixodes is the only genus in and representative of the prostriata, whereas the latter three genera are members of the metastriata. all developmental stages of the prostriata can be easily differentiated from the metastriata using morpholog ...200415682516
the roles of birds, lizards, and rodents as hosts for the western black-legged tick ixodes pacificus.we compared the infestation by ixodid ticks of lizards, rodents, and birds collected simultaneously within areas representing common habitat types in mendocino county, ca. lizards were infested only by ixodes pacificus cooley and kohls, birds by i. pacificus and haemaphysalis leporispalustris (packard), and rodents by i. pacificus, i. spinipalpis hadwen and nuttall, i. woodi bishopp, dermacentor occidentalis marx, and d. variabilis (say). infestation by i. pacificus larvae and nymphs of lizards ...200415709249
identification and characterization of the plasma kallikrein-kinin system inhibitor, haemaphysalin, from hard tick, haemaphysalis longicornis.the plasma kallikrein-kinin system inhibitor, haemaphysalin, from the hard tick, haemaphysalis longicornis, was identified. it was found that haemaphysalin inhibited activation of the plasma kallikrein-kinin system by interfering with reciprocal activation between factor xii and prekallikrein. it did not, however, inhibit amidolytic activities of factor xiia and kallikrein. direct binding assay indicated that factor xii/xiia and high molecular weight kininogen (hk) are the target molecules of ha ...200515711755
ixodid ticks on dogs in southern mozambique.the objective of this study was to determine the species and distribution of ticks infesting domestic dogs in southern mozambique. to this end 89 collections were taken from dogs and the geographic coordinates of the localities at which they were made noted. fifty-eight of these collections were from animals in the city of maputo and 16 from animals at rural settlements close to maputo. a further 15 collections were made from dogs in rural environments north of maputo. ten ixodid tick species, o ...200415732454
immunization of mice with recombinant p27/30 protein confers protection against hard tick haemaphysalis longicornis (acari: ixodidae) infestation.the success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. previously, we cloned a gene encoding 27 kda and 30 kda proteins (p27/30) of haemaphysalis longicornis, and identified the p27/30 is a troponin i-like protein. in this study, the recombinant p27/30 (rp27/30) expressed in escherichia coli was used to immunize mice and the mice were challenge-infested with ticks at different developmental stages of the same species. the rp27 ...200515785123
ixodid tick species attaching to dogs in hungary.a survey was carried out to investigate the occurrence of hard tick species (acari: ixodidae) infesting domestic dogs in hungary. forty veterinary clinics from a wide geographical area were asked to collect hard ticks from dogs and to complete a questionnaire. in total, 25 veterinary clinics submitted 900 ticks from 310 dogs. intensity of infestation ranged from one to 78 per dog. the most preferred sites of tick attachment in decreasing order were head, neck and legs. the majority of ticks (91. ...200515817212
[study on the coinfection of three tick-borne infectious diseases in china using polymerase chain reaction method].to study the existence of ehrluichiosis, lyme disease and tick-borne spotted fever coinfection in some areas in china.200515921584
identification and prevalence of ehrlichia chaffeensis infection in haemaphysalis longicornis ticks from korea by pcr, sequencing and phylogenetic analysis based on 16s rrna gene.genomic dnas extracted from 1,288 haemaphysalis longicornis ticks collected from grass vegetation and various animals from nine provinces of korea were subjected to screening by genus-specific (ehrlichia spp. or anaplasma spp.) real-time taqman pcr and speciesspecific (e. chaffeensis) nested-pcr based on amplification of 16s rrna gene fragments. in all, 611 (47.4%) ticks tested positive for genus-specific amplification of 116 bp fragment of 16s rrna of ehrlichia spp. or anaplasma spp. subsequent ...200515933436
anaemia and deaths in red deer (cervus elaphus) fawns associated with heavy infestations of cattle tick (haemaphysalis longicornis).in late december/early january 1979/80, deaths occurred in 30 red deer (cervus elaphus) fawns. evidence is presented to suggest that these deaths most likely resulted from heavy infestations of ticks (haemaphysalis longicornis).198216030894
theileria orientalis, a blood parasite of cattle. first report in new zealand.a theileria sp. piroplasm has been found in cattle from 10 northland herds. transmission studies, involving two splenectomized calves, led to its identification as t. orientalis, which has not been previously found in new zealand. this piroplasm is relatively benign hut can cause severe anaemia in heavily parasitized animals. the cattle tick haemaphysalis longicornis is considered to be the likely vector.198416031086
a collection of ticks (ixodidae) from wild birds in uruguay.thirty nine wild birds from seven families (alcedinidae, emberizidae, furnariidae, parulidae, trochilidae, turdidae and tyrannidae) were caught from 4 to 7 of february, 2004 at rincón da vassoura (31 degrees 15' s 56 degrees 03' w) department of tacuarembó, uruguay. ninety one nymphs and forty one larvae of ixodidae were recovered from them. the majority of ticks were conventionally identified but 16s mitochondrial rdna sequences were obtained for larvae and nymphs of amblyomma with uncertain sp ...200516132744
contribution of the n-terminal and c-terminal domains of haemaphysalin to inhibition of activation of plasma kallikrein-kinin system.haemaphysalin is a kallikrein-kinin system inhibitor from hard tick haemaphysalis longicornis, and consists of two kunitz type protease inhibitor domains. each domain as well as haemaphysalin inhibited intrinsic coagulation by inhibiting activation of the kallikrein-kinin system without affecting the amidolytic activities of intrinsic coagulation factors, indicating that both domains were involved in the inhibition through a similar mechanism to that for haemaphysalin. reconstitution experiments ...200516169873
efficacy of imidacloprid/permethrin and fipronil/(s)-methoprene combinations against haemaphysalis longicornis ticks evaluated under in vitro and in vivo conditions.haemaphysalis longicornis is one of the most important ticks infesting a wide range of mammals including dogs in japan. h. longicornis is recorded to be a vector of, for example, babesia gibsoni. it was the aim of the study presented here to evaluate the efficacy of imidacloprid/permethrin and fipronil/(s)-methoprene against larval, nymphal and adult stages of h. longicornis under in vitro as well as in vivo conditions. in the in vitro part of the study, ticks showed avoidance behaviour to imida ...200516228268
[detection of the circulation of crimean-congo hemorrhagic fever virus in the piedmont steppes of the north caucasus].the laboratory verified cases of crimean-congo hemorrhagic fever (cchf) in the piedmont steppes of the north caucasus (malgobeksky district, republic of ingushetia) are first described. the source of the first infection was ixodidae ticks; three subsequent sources were contacts with the bloody discharges from patients. cchf virus genome was detected in the blood of the cattle from an epidemic focus and in the pools of the ixodes ticks haemaphysalis parva neum., 1897 and boophilus annulatus say, ...200516250591
the use of a recombinant baculovirus expressing a chitinase from the hard tick haemaphysalis longicornis and its potential application as a bioacaricide for tick control.baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. we have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. the recombinant autographa californica multiple nuclear polyhedrosis virus expressing a chitinase enzyme (acmnpv-cht1) from the hard tick, haemaphysalis longicornis, was constructed and found to have a novel bioacaricidal effect against ticks. the recombinant baculovirus was use ...200616292677
detection of bartonella species from ticks, mites and small mammals in korea.we investigated the prevalence of bartonella infections in ticks, mites and small mammals (rodents, insectivores and weasels) collected during 2001 through 2004, from various military installations and training sites in korea, using pcr and sequence analysis of 16s rrna, 23s rrna and groel heat shock protein genes. the prevalence of bartonella spp. was 5.2% (n = 1,305 sample pools) in ticks, 19.1% (n = 21) in mesostigmatid mites and 13.7% (n = 424 individuals) in small mammals. the prevalence wi ...200516293997
small mammals as hosts of immature ixodid ticks.two hundred and twenty-five small mammals belonging to 16 species were examined for ticks in free state, mpumalanga and limpopo provinces, south africa, and 18 ixodid tick species, of which two could only be identified to genus level, were recovered. scrub hares, lepus saxatilis, and cape hares, lepus capensis, harboured the largest number of tick species. in free state province namaqua rock mice, aethomys namaquensis, and four-striped grass mice, rhabdomys pumilio, were good hosts of the immatu ...200516300195
[the peculiarities of the biology of ticks inhabiting the environs of tomsk city].the presence of ticks ixodes persulcatus and i. pavlovskyi occidentalis is revealed in urban parks and the forests directly adjoined to buildings. i. pavlovskyi occidentalis is found to be the dominant species nearby the city. the both species are occurred almost simultaneously, and the peak of their abundance usually falls on 2nd or 3rd ten-day period of may. in the city the ticks species ceased occurring in registers in the end of may, and in the adjacent forests they disappeared in the end of ...200516316054
random sequencing of cdna library derived from partially-fed adult female haemaphysalis longicornis salivary gland.a cdna library was constructed from salivary glands of partially-fed adult female haemaphysalis longicornis (hard tick). randomly selected clones were sequenced and a total of 633 sequences were analyzed by bioinformatic programs. the sequences were grouped into 213 clusters, with each cluster being considered to be composed of mrnas derived from the same gene or closely related genes. about 36% of the mrna sequences showed significant similarity to known proteins in the non-redundant protein da ...200516327224
molecular cloning and functional characterization of an aspartic protease from the hard tick haemaphysalis longicornis.haemaphysalis longicornis cdna encoding an aspartic protease (longepsin) was identified from a midgut cdna library. the longepsin cdna contains 1176bp that code for 392 amino acid residues with a predictable molecular weight of 39.3kda. the cdna has a signal peptide sequence associated with the n-terminal domains and domain structure analysis revealed that the deduced protein has two aspartic acid residues that are characteristic of a single active site for aspartic proteases. this novel longeps ...200616360947
detection of rickettsial dna in ixodid ticks recovered from dogs and cats in japan.dna from ticks recovered from 1137 dogs and 133 cats from all over japan were examined for rickettsia infection by citrate synthase gene (glta)-based pcr and partial nucleotide sequencing. a total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested pcr. sequence analysis was successfully performed on 102 samples. dna of rickettsia japonica or closely related rickettsia spp. strains were detected from 38 ticks in 16 prefectures mainly in weste ...200516397379
detection of dna of 'candidatus mycoplasma haemominutum' and spiroplasma sp. in unfed ticks collected from vegetation in japan.dna fragments of 'candidatus mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed ixodes ovatus collected from vegetation in hokkaido, fukushima and yamaguchi prefectures, and unfed haemaphysalis flava in yamaguchi prefecture. this finding suggests that ixodid tick is a possible vector of 'c. mycoplasma haemominutum'. spiroplasma dna was also detected from unfed i. ovatus in hokkaido, fukushima and yamaguchi prefectures. the analysis of nucleotides sequence sugge ...200516397391
[ixodidae ticks in bishkek].the fauna of ixodidae ticks was studied in bishkek in 1998-2004. the city is situated in the center of the chuisk valley, at the foot of the kirghiz ridge, at the boundary of a submountain-plain-arid region and a plain-piedmont-semiarid one with steppe fragments. the ticks were gathered on a flag from small mammalians caught by gero traps, from birds, domestic and agricultural animals. a total of 648 animals of 13 species were examined. the bites of ticks were registered in 98 persons in the cit ...200516445237
parasites of the collared peccary from texas.results of a survey of the parasites of the collared peccary (dicotyles tajacu angulatus) in texas are presented. three ectoparasites, amblyomma cajennense, dermacentor variabilis, and pulex porcinus were very common on peccaries from south texas, but less common or absent in arid west texas. sucking lice, pecaroecus javalii, were common on peccaries from west texas, but were not found in south texas. the known range of this louse in texas is extended into the big bend area. two ticks, amnblyomm ...197016509125
identification of a follistatin-related protein from the tick haemaphysalis longicornis and its effect on tick oviposition.the identification of ovary-associated molecules will lead to a better understanding of the physiology of tick reproduction and vector-pathogen interactions. a gene encoding a follistatin-related protein (frp) was obtained by random sequencing from the ovary cdna library of the tick haemaphysalis longicornis. the full-length cdna is 1157 bp, including an intact orf encoding an expected protein with 289 amino acids. three distinct domains were present in the deduced amino acids, namely, the folli ...200616517100
characterization of dog repellent factor from cuticular secretion of female yellow dog tick, haemaphysalis leachi.during its natural life cycle, the yellow dog tick, haemaphysalis leachi, has three hosts, and it has to spend enough time on each of them to complete a blood meal. when irritated, the females of this tick species produce a cuticular secretion that contains a dog-repelling allomone. this improves the tick's chances of survival by deterring the dog from biting the tick off its body. employing response-guided isolation techniques in conjunction with gas chromatography-mass spectrometry, the defens ...200616525874
cross-sectional survey of ixodid tick species on grazing cattle in japan.ixodid tick species were collected from cattle in 60 grazing fields throughout japan. haemaphysalis longicornis was mainly recovered in the western and southern regions, while ixodes species were collected mainly in the central to northern regions. other tick species such as amblyomma testudinarium, boophilus microplus, h. flava and h. kitaokai were identified from a few fields in the central and southern regions. haemaphysalis longicornis were recovered in the fields with higher temperatures an ...200616550336
ticks infesting livestock on farms in western sicily, italy.the results of a 2-year survey on ticks infesting livestock on farms in western sicily, italy, are reported. several dogs living in the same area were also examined for ticks. moreover, free-living ticks from one cattle farm were caught by co(2) traps and flagging. a total of 6208 specimens was collected belonging to 9 species: rhipicephalus bursa (32.4%), rhipicephalus turanicus (22.7%), rhipicephalus sanguineus (19.3%), hyalomma lusitanicum (12.0%), haemaphysalis punctata (7.8%), hyalomma marg ...200616550337
detection of babesia and anaplasma species in rabbits from texas and georgia, usa.rabbits have been shown to harbor a suite of zoonotic organisms, including a babesia species, borrelia burgdorferi, and anaplasma phagocytophilum. in this study, we conducted a molecular survey for various tick-borne pathogens in three species of rabbits from texas and georgia. of 18 black-tailed jackrabbits (lepus californicus) tested from texas, six (28%) were polymerase chain reaction (pcr) positive for babesia, and nucleotide sequencing revealed two distinct species or strains. two jackrabbi ...200616584322
potential risk of pathogen transmission by acaricide-poisoned ticks.many aspects of tick poisoning with acaricides have yet to be elucidated. one of them is the influence of acaricide poisoning on tick infectivity to their hosts. to clarify this problem, we should know how tick poisoning develops after acaricide application. data obtained during more than three decades of work with ticks of various species and with acaricides of different groups are presented in the paper. the first important phenomenon found was the gradual and progressive development of toxic ...200616597511
rickettsiae of the spotted-fever group in ixodid ticks from hungary: identification of a new genotype ('candidatus rickettsia kotlanii').three common european 'anthrophilic' ticks, ixodes ricinus, haemaphysalis concinna and dermacentor reticulatus, were collected in hungary and tested, in assays based on nested pcr, for rickettsiae of the spotted-fever group. low percentages of i. ricinus (2.7%) and h. concinna (1.0%) and a high percentage of d. reticulatus (26.8%) were found to be infected. the rickettsiae in the ticks were then identified, by sequencing of the genes coding for 16s ribosomal rna (16s rdna), citrate synthase (glt ...200616630380
tick troponin i-like molecule is a potent inhibitor for angiogenesis.this is the first report identifying the anti-angiogenesis saliva molecule of the ixodid tick. in our previous study, we identified a troponin i-like molecule (hltni) of the ixodid hard tick haemaphysalis longicornis, a vector for various pathogens. to investigate its potential inhibitory effects for angiogenesis, we expressed and purified recombinant hltni in escherichia coli. in a vascular endothelial growth factor (vegf) competitive angiogenesis assay, the recombinant hltni significantly inhi ...200616631826
efficacy of orally administered powdered aloe juice (aloe ferox) against ticks on cattle and ticks and fleas on dogs.the efficacy of orally administered powdered aloe juice (aloe ferox) was evaluated against ticks on cattle and against ticks and fleas on dogs. twelve calves were each infested over a 25-day period with approximately 4000 larvae of rhipicephalus (boophilus) decoloratus and allocated to 3 groups of 4 calves each. three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg/kg body weight and those in another 25 mg/kg body weight of powdered al ...200516642714
ixodid ticks parasitizing iberian red deer (cervus elaphus hispanicus) and european wild boar (sus scrofa) from spain: geographical and temporal distribution.commercial hunting of spanish wild ungulates has made them an important economic resource. wild ungulates may have an important role in the maintenance of ixodid tick populations, and also as reservoirs of pathogens. we studied the ixodid ticks that parasitize iberian red deer and european wild boar from spain. ixodid ticks (n=6,336) were collected from 431 iberian red deer and 142 wild boar in different regions of spain. we found 10 different ixodid tick species parasitizing iberian red deer, m ...200616675125
a novel gene encoding a thrombin inhibitory protein in a cdna library from haemaphysalis longicornis salivary gland.a novel thrombin inhibitory protein coding gene was identified from a cdna library derived from salivary gland of partially-fed haemaphysalis longicornis (hard tick). the gene encoded a 93-amino acid protein, designated chimadanin, which had a signal peptide sequence and was predicted to be a secretory protein. it showed no similarity to any other previously identified proteins or conserved domain sequences. the gene was expressed during blood feeding and suggested to be expressed mainly in the ...200616757887
risk factors associated with ixodid tick species distributions in the basque region in spain.ixodid tick abundance was investigated in the basque region in spain in two 1-year longitudinal studies, in 1992-1993 and 2003-2004. forty zones were visited monthly and 162 672 ticks (87% larvae, 12% nymphs and 1% adults) were collected by blanket dragging. eleven tick species belonging to the genera ixodes, haemaphysalis, rhipicephalus and dermacentor were identified including haemaphysalis concinna koch, which had not previously been reported in spain. tick species abundance differed between ...200616796614
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