in vitro isolation of entomophthora muscae (entomophthorales: entomophthoraceae) from infected seed corn maggot delia platura (diptera: anthomyiidae). 19902319161
isolation and pathogenicity of serratia marcescens from adult house flies infected with entomophthora muscae. 19902405062
entomophthora muscae resting spore formation in vivo in the host delia radicum.the formation in vivo of entomophthora muscae resting spores was investigated in the host, delia radicum (cabbage root fly), by analysis of field data on the seasonal occurrence of e. muscae resting spores over 4 years. e. muscae resting spores in d. radicum were spherical with an average diameter of 39.4 microm, and the average numbers produced were estimated at 5.7 x 10(4) resting spores/female cadaver. resting spores were found only after midsummer in d. radicum and almost exclusively in fema ...200011023736
entomopathogenic fungi in flies associated with pastured cattle in denmark.cattle flies, including musca autumnalis, haematobia irritans, and hydrotaea irritans, are pests of pastured cattle. a 2-year study of the natural occurrence of entomopathogenic fungi in adult cattle flies and other flies associated with pastures showed that the four species included in the entomophthora muscae species complex (e. muscae sensu lato) caused high infection levels in several species of flies. however, only a few specimens of cattle flies were infected by e. muscae sensu stricto des ...200111356054
entomophthora ferdinandii (zygomycetes: entomophthorales) causing natural infections of musca domestica (diptera: muscidae) in argentina.the identity and activity of an entomopathogenic fungus belonging to the entomophthora muscae species complex and infecting musca domestica in poultry houses from la plata, argentina, is reported. entomophthora caused natural infections between september 2001 and september 2003. primary conidia of this fungus were on average 29.5 +/- 1.2 x 23.4 +/- 2.4 microm and contained, on average, 10.5 +/- 0.1 nuclei (range: 7-15) with an average diameter of 4.8 +/- 0.1 microm. this fungus is identified as ...200616552490
value of host range, morphological, and genetic characteristics within the entomophthora muscae species complex.entomopthora muscae sensu lato is a complex of morphologically similar fungal species pathogenic to evolutionarily advanced flies (cyclorrhapha). to reach an operational species definition and recognition of species within this complex, the values of host range, morphological and genetic characteristics are reconsidered. within the e. muscae species complex morphological and nuclear characteristics of the primary conidia are taxonomically important. in this study we compared the dimensions and n ...200616905302
application of nested-pcr technique to resting spores from the entomophthora muscae species complex: implications for analyses of host-pathogen population interactions.we developed new entomophthora-specific primers for nested-pcr of the its ii region to be used on in vivo material and combined it with rflp. resting spores from scathophaga stercoraria (3 specimens), delia radicum (9 specimens), botanophila fugax (1 specimen), and two syrphid host species, platycheirus peltatus and melanostoma mellinum (one specimen of each) were characterized genetically after analysis of rflp-profiles of the pcr-products. the genetic characterization of the resting spore isol ...200221156553
sequential utilization of hosts from different fly families by genetically distinct, sympatric populations within the entomophthora muscae species complex.the fungus entomophthora muscae (entomophthoromycota, entomophthorales, entomophthoraceae) is a widespread insect pathogen responsible for fatal epizootic events in many dipteran fly hosts. during epizootics in 2011 and 2012 in durham, north carolina, we observed a transition of fungal infections from one host, the plant-feeding fly delia radicum, to a second host, the predatory fly coenosia tigrina. infections first appeared on delia in the middle of march, but by the end of may, coenosia compr ...201323951101
intraspecific variation and host specificity of entomophthora muscae sensu stricto isolates revealed by random amplified polymorphic dna, universal primed pcr, pcr-restriction fragment length polymorphism, and conidial morphology.the intraspecific variations of entomophthora muscae s. str. associated with particular host species, musca domestica and delia radicum, sampled from different localities and different years in denmark and the variation of e. muscae s. str. originating from different host taxa were investigated. the isolates were compared both by primary spore morphology and by three molecular methods: random amplified polymorphic dna, universal primed pcr, and pcr-restriction fragment length polymorphism. analy ...200112009807
distance of conidial discharge of entomophthora muscae and entomophthora schizophorae (zygomycotina: entomophthorales)the distance traveled by conidia of the e. muscae species complex from cadavers of musca domestica was measured. laboratory strains of e. muscae and e. schizophorae, as well as a field-collected strain of e. muscae, were compared in darkness under still air conditions at 21&deg;c. most conidia were discharged <3.75 cm from the cadaver, with a range of 0 to 8.75 cm. when a fly cadaver was affixed to a vertical surface (normal death orientation), conidia discharged from the dorsum of the abdomen t ...19968812606
comparative transcriptomics reveal host-specific nucleotide variation in entomophthoralean fungi.obligate parasites are under strong selection to increase exploitation of their host to survive while evading detection by host immune defences. this has often led to elaborate pathogen adaptations and extreme host specificity. specialization on one host, however, often incurs a trade-off influencing the capacity to infect alternate hosts. here, we investigate host adaptation in two morphologically indistinguishable and closely related obligate specialist insect-pathogenic fungi from the phylum ...201627717247
effect of the entomopathogenic fungus, entomophthora muscae (zygomycetes: entomophthoraceae), on sex pheromone and other cuticular hydrocarbons of the house fly, musca fly (musca domestica) males are highly attracted to dead female flies infected with the entomopathogenic fungus entomophthora muscae. because males orient to the larger abdomen of infected flies, both visual and chemical cues may be responsible for the heightened attraction to infected flies. our behavioral assays demonstrated that the attraction is sex-specific-males were attracted more to infected females than to infected males, regardless of cadaver size. we examined the effect of e. mu ...200212384083
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