Publications

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biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in pichia pastoris.phytases catalyze the cleavage of phosphate groups from phytic acid. here, we have studied the effects of glycosylation on the properties of aspergillus japonicus c03 phytase expressed in pichia pastoris. the enzyme orf of 1338 nucleotides was cloned from genomic dna, and encoded a secreted mature protein of 446 amino acids, which included the sequence motif rhgxrx and dipeptide hd, classifying the phytase as a histidine acid phosphate. after transformation and 72h of induction, p.pastoris gs115 ...201424680732
comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with aspergillus japonicus pj01.submerged fermentation (smf) and solid-state fermentation (ssf) of aspergillus japonicus pj01 for multi-enzyme complexes (mec) production were comparatively studied. the results showed that orange peel and wheat bran were the best substrates for mec production in smf and ssf, respectively. after 72 h of cultivation under smf, the maximal pectinase, cmcase, and xylanase activities reached 2610, 85, and 335 u/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under ssf, ...201526260269
semirational directed evolution of loop regions in aspergillus japonicus β-fructofuranosidase for improved fructooligosaccharide production.the aspergillus japonicus β-fructofuranosidase catalyzes the industrially important biotransformation of sucrose to fructooligosaccharides. operating at high substrate loading and temperatures between 50 and 60°c, the enzyme activity is negatively influenced by glucose product inhibition and thermal instability. to address these limitations, the solvent-exposed loop regions of the β-fructofuranosidase were engineered using a combined crystal structure- and evolutionary-guided approach. this semi ...201526253664
effects of surfactants and microwave-assisted pretreatment of orange peel on extracellular enzymes production by aspergillus japonicus pj01.the effects of surfactants and microwave pretreatment of orange peel powder (opp) on the production of pectinase, cellulase, and xylanase by aspergillus japonicus pj01 in submerged fermentation were investigated. the results showed that when opp was pretreated only by microwave (630 w, 9 min, and liquid/solid ratio 5), the activities of exo-pectinase, carboxymethyl cellulase (cmcase), xylanase, and filter paper cellulase (fpase) were increased by 11.8, 20.6, 16.2, and 24.0 %, respectively, and w ...201525920331
economic analysis and environmental impact assessment of three different fermentation processes for fructooligosaccharides production.three different fermentation processes for the production of fructooligosaccharides (fos) were evaluated and compared in terms of economic aspects and environmental impact. the processes included: submerged fermentation of sucrose solution by aspergillus japonicus using free cells or using the cells immobilized in corn cobs, and solid-state fermentation (ssf) using coffee silverskin as support material and nutrient source. the scale-up was designed using data obtained at laboratory scale and con ...201526433793
effect of water activity, temperature, and mixed fungal spore interactions on ochratoxin a production by aspergillus carbonarius.the purpose of this work was to investigate the potential of two nontoxigenic aspergillus section nigri species (aspergillus tubingensis and aspergillus japonicus) to influence the in vitro ochratoxin a (ota) production of three toxigenic aspergillus carbonarius isolates (ac-28, ac-29, and ac-33) from greek vineyards of different geographical areas. ota accumulation was evaluated by inoculation of 0:100, 25:75, 50:50, 75:25, and 100:0 ratios of mixed spore suspensions on a synthetic grape juice ...201525710154
diversity of black aspergilli isolated from raisins in argentina: polyphasic approach to species identification and development of scar markers for aspergillus ibericus.aspergillus section nigri is a heterogeneous fungal group including some ochratoxin a producer species that usually contaminate raisins. the section contains the series carbonaria which includes the toxigenic species aspergillus carbonarius and nontoxigenic aspergillus ibericus that are phenotypically undistinguishable. the aim of this study was to examine the diversity of black aspergilli isolated from raisins and to develop a specific genetic marker to distinguish a. ibericus from a. carbonari ...201526114593
survey of philippine coffee beans for the presence of ochratoxigenic fungi.in 2012 to 2014, philippine green coffee beans from coffea arabica in benguet and ifugao; coffea canephora var. robusta in abra, cavite, and ifugao; and coffea liberica and coffea excelsea from cavite were collected and assessed for the distribution of fungi with the potential to produce ochratoxin a (ota). the presence of fungal species was evaluated both before and after surface sterilization. there were remarkable ecological and varietal differences in the population of ota-producing species ...201626814977
highly efficient synthesis of fructooligosaccharides by extracellular fructooligosaccharide-producing enzymes and immobilized cells of aspergillus aculeatus m105 and purification and biochemical characterization of a fructosyltransferase from the fungus.in this work, aspergillus aculeatus m105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. the maximum yields of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. a fructosyltransferase (ftase), aaft32a, was purified from m105. the optimal ph and temperature of aaft32a were ph 5.0-6.0 and 65 °c, respectively. the km, vmax, and kcat values for the transfructosylating activity of a ...201627492129
saccharification of orange peel wastes with crude enzymes from new isolated aspergillus japonicus pj01.this study investigated the saccharification of orange peel wastes with crude enzymes from aspergillus japonicus pj01. pretreated orange peel powder was hydrolyzed by submerged fermentation (smf) and solid-state fermentation (ssf) crude enzymes, the results showed that 4 % (w/v) of solid loading, undiluted crude enzymes, and 45 °c were suitable saccharification conditions. the hydrolysis kinetics showed that the apparent michaelis-menten constant [formula: see text] and maximal reaction rate [fo ...201626718204
effect of cd⁺² on phosphate solubilizing abilities and hydrogen peroxide production of soil-borne micromycetes isolated from phragmites australis-rhizosphere.the aims of this work were to evaluate the phosphate-solubilization and hydrogen peroxide (h2o2) production by the soil-borne micromycetes, aspergillus japonicus, penicillium italicum and penicillium dipodomyicola, isolated from phragmites australis rhizosphere and to study the effect of several concentrations of cadmium (cd(2+)) on both variables. our results showed that p. italicum achieved a higher p-solubilization and h2o2 production than a. japonicus and p. dipodomyicola, as only p. italicu ...201626646403
potential application of waste from castor bean (ricinus communis l.) for production for xylanase of interest in the industry.xylanases activity (xy) from aspergillus japonicus urm5620 produced by solid-state fermentation (ssf) of castor press cake (ricinus communis) on different conditions of production and extraction by peg/citrate aqueous two-phase system (atps) were investigated. xy production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), ph (4.0-6.0) and temperature (25-35 °c), obtaining the maximum activity of 29,085 ± 1808 u g ds(-1) using 5.0 g of substrate with initial moisture of 15 ...201628330216
biochemical effect of a histidine phosphatase acid (phytase) of aspergillus japonicus var. saito on performance and bony characteristics of broiler.phytases are enzymes that hydrolyze the ester linkage of phytic acid, releasing inositol and inorganic phosphate. the phytic acid (phytate) is a major form of phosphorus in plant foods. knowing that diet for animal of production has the cereal base (corn and soybean), primarily, broilers need for an alternative to use of the phosphate present in these ingredients, since it does not naturally produce the enzyme phytase, which makes it available. the aims of this work was studding the safe supplem ...201627625972
structural characterization of eash (aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.aj_eash is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. the three dimensional structure of aj_eash (2.2 å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.201627885368
secondary metabolites from aspergillus japonicus cam231, an endophytic fungus associated with garcinia preussii.chemical investigation of aspergillus japonicus cam231, isolated from the leaf of garcina preussii collected in cameroon, yielded two new compounds; one pyrone derivative, hydroxy neovasinin (1) and one phenol derivative, asperolan (2), together with two known compounds neovasifurarone b (3) and variecolin (4). the structures of the two new compounds were established using intensive nmr spectroscopy and hrms spectra in comparison with data found in literature. the structure of compound 1 was con ...201727806646
extracellular secretion of phytase from transgenic wheat roots allows utilization of phytate for enhanced phosphorus uptake.a significant portion of organic phosphorus comprises of phytates which are not available to wheat for uptake. hence for enabling wheat to utilize organic phosphorus in form of phytate, transgenic wheat expressing phytase from aspergillus japonicus under barley root-specific promoter was developed. transgenic events were initially screened via selection media containing basta, followed by pcr and basta leaf paint assay after hardening. out of 138 successfully regenerated to events, only 12 had c ...201728667571
purification and functional properties of a novel glucoamylase activated by manganese and lead produced by aspergillus japonicus.microbial amylases are used to produce ethanol, glucose and can be applied in textiles products, detergents and other industries. this study aimed to determine the best carbon source concentration to induce the amylase production by a. japonicus, and its purification and biochemical characterization. for that, this fungus was cultivated in khanna medium, ph 5.5, for 4 days, at 25°c, in static condition, supplemented with potato starch and maltose in different concentrations. the fungal crude enz ...201728412339
production of the functional trisaccharide 1-kestose from cane sugar molasses using aspergillus japonicus β-fructofuranosidase.we report the production of the functional trisaccharide 1-kestose, o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside, by β-fructofuranosidase from aspergillus japonicus using sugar cane molasses as substrate. sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. the tetrasaccharide nystose, o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside, was produced from 1-kestose. cane sugar molasses mixed with w ...201727803952
development of a novel compound microbial agent for degradation of kitchen waste.large quantities of kitchen waste are produced in modern society and its disposal poses serious environmental and social problems. the aim of this study was to isolate degradative strains from kitchen waste and to develop a novel and effective microbial agent. one hundred and four strains were isolated from kitchen waste and the 84 dominant strains were used to inoculate protein-, starch-, fat- and cellulose-containing media for detecting their degradability. twelve dominant strains of various s ...201728279600
collagenase produced from aspergillus sp. (ucp 1276) using chicken feather industrial residue.an extracellular collagenolytic serine protease was purified from aspergillus sp., isolated from the caatinga biome in northeast brazil by a two-step chromatographic procedure, using an anion-exchanger and gel filtration. the enzyme was produced by submerged fermentation of feather residue as a substrate. the purified collagenase showed a 2.09-fold increase in specific activity and 22.85% yield. the enzyme was a monomeric protein with a molecular mass of 28.7 kda, estimated by an sds-page and ak ...201727808430
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens.filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear.201728100314
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