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fungal biodegradation of phthalate plasticizer in situ.this unique study describes how aspergillus japonicus, penicillium brocae and purpureocillium lilacinum, three novel isolates of our laboratory from heavily plastics-contaminated soil completely utilized the plasticizer di(2-ethylhexyl)phthalate (dehp) bound to pvc blood storage bags (bb) in simple basal salt medium (bsm) by static submerged growth (28 °c). initial quantification as well as percentage utilization of dehp blended to bb were estimated periodically by extracting it into n-hexane. a ...201322903609
transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains.phytate is a major constituent of wheat seeds and chelates metal ions, thus reducing their bioavailability and so the nutritional value of grains. transgenic plants expressing heterologous phytase are expected to enhance degradation of phytic acid stored in seeds and are proposed to increase the in vitro bioavailability of mineral nutrients. wheat transgenic plants expressing aspergillus japonicus phytase gene (phya) in wheat endosperm were developed till t3 generation. the transgenic lines exhi ...027687031
the important ergot alkaloid intermediate chanoclavine-i produced in the yeast saccharomyces cerevisiae by the combined action of easc and ease from aspergillus japonicus.ergot alkaloids are a group of highly bioactive molecules produced by a number of filamentous fungi. these compounds have been intensely studied for decades, mainly due to their deleterious effects in contaminated food and feeds, but also for their beneficial pharmaceutical and agricultural applications. biosynthesis of ergot alkaloids goes via the common intermediate chanoclavine-i, and studies of the key enzymes, ease and easc, involved in chanoclavine-i formation, have relied on gene compleme ...201425112180
continuous production of high-purity fructooligosaccharides and ethanol by immobilized aspergillus japonicus and pichia heimii.high-purity fructooligosaccharides (fos) were produced from sucrose by an innovative process incorporating immobilized aspergillus japonicus and pichia heimii cells. intracellular ftase of a. japonicus converted sucrose into fos and glucose, and p. heimii fermented glucose mainly into ethanol. the continuous production of fos was carried out using a tanks-in-series bioreactor consisting of three stirred tanks. when a solution composed of 1 g l(-1) yeast extract and 300 g l(-1) sucrose was fed co ...201323568753
biochemical effect of a histidine phosphatase acid (phytase) of aspergillus japonicus var. saito on performance and bony characteristics of broiler.phytases are enzymes that hydrolyze the ester linkage of phytic acid, releasing inositol and inorganic phosphate. the phytic acid (phytate) is a major form of phosphorus in plant foods. knowing that diet for animal of production has the cereal base (corn and soybean), primarily, broilers need for an alternative to use of the phosphate present in these ingredients, since it does not naturally produce the enzyme phytase, which makes it available. the aims of this work was studding the safe supplem ...201627625972
biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in pichia pastoris.phytases catalyze the cleavage of phosphate groups from phytic acid. here, we have studied the effects of glycosylation on the properties of aspergillus japonicus c03 phytase expressed in pichia pastoris. the enzyme orf of 1338 nucleotides was cloned from genomic dna, and encoded a secreted mature protein of 446 amino acids, which included the sequence motif rhgxrx and dipeptide hd, classifying the phytase as a histidine acid phosphate. after transformation and 72h of induction, p.pastoris gs115 ...201424680732
effects of aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages.cellulose and hemicellulose are quantitatively the most important structural carbohydrates present in ruminant diets. rumen micro-organisms produce enzymes that catalyse their hydrolysis, but the complex network formed by structural carbohydrates and lignin reduces their digestibility and restricts efficient utilisation of feeds by ruminants. this study aimed to produce two enzymatic extracts, apply them in ruminant diets to determine the best levels for ruminal digestibility and evaluate their ...201222508186
[isolation of aspergillus section nigri strains in yerba mate in posadas (misiones, argentina) and evaluation of their ochratoxigenic potential].the objectives of the present work were to investigate the isolation frequency of genus aspergillus in canchada yerba mate (ymch) and elaborated yerba mate (yme) (ilex paraguariensis) and the proportion of section nigri isolates, as well as to determine ochratoxin a production by aspergillus species section nigri. three hundred twenty eight aspergillus strains from 20 samples of ymch and 1306 aspergillus strains from 36 samples of yme were isolated; of the total, 279 from the first group of stra ...201323876265
purification and properties ofβ-fructofuranosidase from aspergillus japonicus.β-fructofuranosidase fromaspergillus japonicus, which produces 1-kestose (o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside) and nystose (o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside) from sucrose, was purified to homogeneity by fractionation with calcium acetate and ammonium sulphate and chromatography with deae-cellulofine and sephadex g-200. its molecular size was estimated to be about 304,000 da by gel filtration. the enzyme ...199224425477
secondary metabolites from aspergillus japonicus cam231, an endophytic fungus associated with garcinia preussii.chemical investigation of aspergillus japonicus cam231, isolated from the leaf of garcina preussii collected in cameroon, yielded two new compounds; one pyrone derivative, hydroxy neovasinin (1) and one phenol derivative, asperolan (2), together with two known compounds neovasifurarone b (3) and variecolin (4). the structures of the two new compounds were established using intensive nmr spectroscopy and hrms spectra in comparison with data found in literature. the structure of compound 1 was con ...201727806646
production of the functional trisaccharide 1-kestose from cane sugar molasses using aspergillus japonicus β-fructofuranosidase.we report the production of the functional trisaccharide 1-kestose, o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside, by β-fructofuranosidase from aspergillus japonicus using sugar cane molasses as substrate. sucrose in cane sugar molasses acted as a fructosyl donor and acceptor for the enzyme. the tetrasaccharide nystose, o-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl-(2→1)-β-d-fructofuranosyl α-d-glucopyranoside, was produced from 1-kestose. cane sugar molasses mixed with w ...201727803952
saccharification of orange peel wastes with crude enzymes from new isolated aspergillus japonicus pj01.this study investigated the saccharification of orange peel wastes with crude enzymes from aspergillus japonicus pj01. pretreated orange peel powder was hydrolyzed by submerged fermentation (smf) and solid-state fermentation (ssf) crude enzymes, the results showed that 4 % (w/v) of solid loading, undiluted crude enzymes, and 45 °c were suitable saccharification conditions. the hydrolysis kinetics showed that the apparent michaelis-menten constant [formula: see text] and maximal reaction rate [fo ...201626718204
potential application of waste from castor bean (ricinus communis l.) for production for xylanase of interest in the industry.xylanases activity (xy) from aspergillus japonicus urm5620 produced by solid-state fermentation (ssf) of castor press cake (ricinus communis) on different conditions of production and extraction by peg/citrate aqueous two-phase system (atps) were investigated. xy production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), ph (4.0-6.0) and temperature (25-35 °c), obtaining the maximum activity of 29,085 ± 1808 u g ds(-1) using 5.0 g of substrate with initial moisture of 15 ...201628330216
economic analysis and environmental impact assessment of three different fermentation processes for fructooligosaccharides production.three different fermentation processes for the production of fructooligosaccharides (fos) were evaluated and compared in terms of economic aspects and environmental impact. the processes included: submerged fermentation of sucrose solution by aspergillus japonicus using free cells or using the cells immobilized in corn cobs, and solid-state fermentation (ssf) using coffee silverskin as support material and nutrient source. the scale-up was designed using data obtained at laboratory scale and con ...201526433793
comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with aspergillus japonicus pj01.submerged fermentation (smf) and solid-state fermentation (ssf) of aspergillus japonicus pj01 for multi-enzyme complexes (mec) production were comparatively studied. the results showed that orange peel and wheat bran were the best substrates for mec production in smf and ssf, respectively. after 72 h of cultivation under smf, the maximal pectinase, cmcase, and xylanase activities reached 2610, 85, and 335 u/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under ssf, ...201526260269
semirational directed evolution of loop regions in aspergillus japonicus β-fructofuranosidase for improved fructooligosaccharide production.the aspergillus japonicus β-fructofuranosidase catalyzes the industrially important biotransformation of sucrose to fructooligosaccharides. operating at high substrate loading and temperatures between 50 and 60°c, the enzyme activity is negatively influenced by glucose product inhibition and thermal instability. to address these limitations, the solvent-exposed loop regions of the β-fructofuranosidase were engineered using a combined crystal structure- and evolutionary-guided approach. this semi ...201526253664
effects of surfactants and microwave-assisted pretreatment of orange peel on extracellular enzymes production by aspergillus japonicus pj01.the effects of surfactants and microwave pretreatment of orange peel powder (opp) on the production of pectinase, cellulase, and xylanase by aspergillus japonicus pj01 in submerged fermentation were investigated. the results showed that when opp was pretreated only by microwave (630 w, 9 min, and liquid/solid ratio 5), the activities of exo-pectinase, carboxymethyl cellulase (cmcase), xylanase, and filter paper cellulase (fpase) were increased by 11.8, 20.6, 16.2, and 24.0 %, respectively, and w ...201525920331
properties of aspergillus japonicus β-fructofuranosidase immobilized on porous silica.β-fructofuranosidase from aspergillus japonicus mu-2, which produces fructo-oligosaccharides (1-kestose: o-β-d-fructofuranosyl-(2 → 1)-β-d-fructofuranosyl α-d-glucopyranoside); and nystose: o-β-d-fructofuranosyl-(2 → 1)-β-d-fructofuranosyl-(2 → 1)-β-d-fructofuranosyl α-d-glucopyranoside) from sucrose, was immobilized, covalently with glutaraldehyde onto alkylamine porous silica, at high efficiency (64%). optimum pore diameter of porous silica for immobilization of the enzyme was 91.7 nm. after i ...199324419951
extracellular secretion of phytase from transgenic wheat roots allows utilization of phytate for enhanced phosphorus uptake.a significant portion of organic phosphorus comprises of phytates which are not available to wheat for uptake. hence for enabling wheat to utilize organic phosphorus in form of phytate, transgenic wheat expressing phytase from aspergillus japonicus under barley root-specific promoter was developed. transgenic events were initially screened via selection media containing basta, followed by pcr and basta leaf paint assay after hardening. out of 138 successfully regenerated to events, only 12 had c ...201728667571
purification and functional properties of a novel glucoamylase activated by manganese and lead produced by aspergillus japonicus.microbial amylases are used to produce ethanol, glucose and can be applied in textiles products, detergents and other industries. this study aimed to determine the best carbon source concentration to induce the amylase production by a. japonicus, and its purification and biochemical characterization. for that, this fungus was cultivated in khanna medium, ph 5.5, for 4 days, at 25°c, in static condition, supplemented with potato starch and maltose in different concentrations. the fungal crude enz ...201728412339
structural characterization of eash (aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.aj_eash is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. the three dimensional structure of aj_eash (2.2 å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.201627885368
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