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rahn-2, a chromosomal extended-spectrum class a beta-lactamase from rahnella aquatilis.rahnella aquatilis is an environmental enterobacterial species with a chromosomal bla(rahn-1) gene encoding extended-spectrum class a beta-lactamase rahn-1. we describe the diversity of bla(rahn) genes from two groups of strains, g1 and g2, isolated from raw fruits and vegetables, and the new class a beta-lactamase rahn-2.201020511369
identification of effective pb resistant bacteria isolated from lens culinaris growing in lead contaminated soils.soil bacteria are a new phytoremediation system for the removal of heavy metals from soils. in this study, fifteen soil bacteria were isolated from root nodules of lentil growing in heavy metals contaminated soils, particularly by lead. molecular characterization of the collection showed a large diversity, including agrobacterium tumefaciens, rahnella aquatilis, pseudomonas, and rhizobium sp. these soil bacteria had a wide range of tolerance to heavy metals. among them, strains of a. tumefaciens ...201524740715
culture-dependent and -independent methods to investigate the predominant microorganisms associated with wet processed coffee.the fermentation process of yunnan arabica coffee is a typical wet fermentation. its excellent quality is closely related to microbes in the process of fermentation. the purpose of this study was to isolate and identify the microorganisms in the wet method of coffee processing in yunnan province, china. microbial community structure and dominant bacterial species were evaluated by traditional cultivated separation method and pcr-dgge technology, and were further analyzed in combination with the ...201627113591
extended spectrum ß-lactamase- and constitutively ampc-producing enterobacteriaceae on fresh produce and in the agricultural environment.the attribution of fresh produce to the overall community-associated exposure of humans to esbl- or ampc-producing bacteria is currently unknown. to address this issue, the prevalence of esbl- and ampc-producing enterobacteriaceae on fresh produce produced in the netherlands was determined. seven vegetable types that are consumed raw were selected: blanched celery, bunched carrots, chicory, endive, iceberg lettuce, mushrooms, and radish. the vegetables were mostly obtained from supermarkets. to ...201424211774
etude taxonomique de souches appartenant ou apparentées au genre erwinia, groupe herbicola, et à l'espece enterobacter agglomerans.the classification of 169 strains related to the species enterobacter agglomerans or erwinia berbicola has been carried out by numerical procedure. the taxonomic position of the strains is discussed in relation to the species enterobacter sakazakii, rahnella aquatilis, escherichia adecarboxylata. five principal groups can be defined. the ent. agglomerans or e. herbicola species can be divided into 15 subgroups. some of the ent. agglomerans strains are phenotypically related to the ent. sakazakii ...198323194595
enumeration and identification of coliform bacteria injured by chlorine or fungicide mixed with agricultural water.chemical sanitizers may induce no injury (bacteria survive), sublethal injury (bacteria are injured), or lethal injury (bacteria die). the proportion of coliform bacteria that were injured sublethally by chlorine and fungicide mixed with agricultural water (pond water), which was used to dilute the pesticide solution, was evaluated using the thin agar layer (tal) method. in pure cultures of enterobacter cloacae , escherichia coli , and e. coli o157:h7 (representing a human pathogen), the percent ...201628221856
environmental isolate of rahnella aquatilis harbors class 1 integron.the paper presents first description of class 1 integron in an environmental strain of rahnella aquatilis, a rarely isolated gram-negative bacterium of the family enterobacteriaceae. the strain was isolated from the warta river water, poland. class 1 integrase gene was detected by a pcr assay. sequencing of the integron's variable region showed the presence of a dfra1-aada1 gene cassette array. the integron was located in a 54-kbp plasmid that was transferable to escherichia coli j-53 recipient ...201626424139
phosphate solubilization potential and modeling of stress tolerance of rhizobacteria from rice paddy soil in northern iran.the purposes of this study were to evaluate the phosphate solubilization activity of bacteria isolated from the rhizosphere of rice paddy soil in northern iran, and to study the effect of temperature, nacl and ph on the growth of these isolates by modeling. three of the most effective strains from a total of 300 isolates were identified and a phylogenetic analysis was carried out by 16s rdna sequencing. the isolates were identified as pantoea ananatis (m36), rahnella aquatilis (m100) and enterob ...201424894539
evaluation of phosphate-solubilizing bacteria on the growth and grain yield of rice (oryza sativa l.) cropped in northern iran.this study aimed to evaluate the efficiency of four phosphate-solubilizing bacteria (psb) on the growth and yield of rice under different soil conditions.201526294004
isolation and identification of phytate-degrading rhizobacteria with activity of improving growth of poplar and masson pine.a number of soil microorganisms can convert insoluble forms of phosphorus (p) to an accessible form to increase plant yields. phytate is such a large kind of insoluble organic phosphorus that plants cannot absorb directly in soil, so the objectives of this study were to isolate, screen phytate-degrading rhizobacteria (pdrb), and to select potential microbial inocula that could increase the p uptake by plants. in this study, a total of 24 soil samples were collected from natural habitats of eight ...201323709169
isolation of hydrocarbon-degrading and biosurfactant-producing bacteria and assessment their plant growth-promoting traits.forty-two hydrocarbon-degrading bacterial strains were isolated from the soil heavily contaminated with petroleum hydrocarbons. forty-one strains were identified based on their whole-cell fatty acid profiles using the midi-mis method. thirty-three of them belong to species rhodococcus erythropolis, while the others to the genera rahnella (4), serratia (3) and proteus (1). isolates were screened for their ability to produce biosurfactants/bioemulsifiers. for all of them the activity of several me ...201626708648
co-transmission of rahnella aquatilis between hospitalized patients.rahnella aquatilis is an environmental gram-negative bacillus that is rarely reported as human pathogen, being mainly associated with infections in immunocompromised patients. herein we describe two cases of r. aquatilis isolates recovered from endotracheal aspirate cultures of different patients in a tertiary hospital located in the city of são paulo, brazil. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16s rdna gene sequencing were performed to confirm bacte ...201526410540
[biological activity of native and modified lipopolysaccharides rahnella aquatilis].the results of the comparative toxicity studies of native lipopolysaccharide (lps) of rahnella aquatilis 96u037 and that modified by tin complexes indicates that, due to the modification of lps by tin complex with benzoylhydrazone of 4-dimethylaminobenzaldehyde, a decrease of its toxicity was observed that led to disappearance of the pyrogenic effect. all obtained derivatives lost completely the antigenic activity both in homologous and heterologous systems which may indicate to the interaction ...201122308745
inoculation of lens culinaris with pb-resistant bacteria shows potential for phytostabilization.phytoremediation comprises a set of plant and microbe-based technologies for remediation of soil heavy metal contamination. in this work, four pb-resistant bacteria (agrobacterium tumefaciens, rahnella aquatilis, and two pseudomonas sp.) were selected among a collection of isolates from root nodule of lens culinaris. they had a high degree of bioaccumulation ability in nutrient medium containing 2 mm pb, and the maximum pb accumulation of whole cell was found after 48-h incubation. these pb-resi ...201525185494
identification of tetrodotoxin-producing bacteria from goby yongeichthys criniger.toxic goby fish (yongeichthys criniger) containing tetrodotoxin (ttx), from zhanjiang, guangdong province, china, were screened for ttx-producing bacteria. two toxic bacterial strains were isolated from the liver of y. criniger and respectively denoted xc3-3 and xl-1. ttx production by the strains was confirmed by mouse bioassay, enzyme-linked immunosorbent assay and high performance liquid chromatography coupled with mass spectrometry. based on morphological, physiological and biochemical chara ...201526238172
culture-dependent bacteria in commercial fishes: qualitative assessment and molecular identification using 16s rrna gene sequencing.fish contamination has been extensively investigated along the saudi coasts, but studies pertaining to bacterial pathogens are scarce. we conducted qualitative assessment and molecular identification of culture-dependent bacteria in 13 fish species from three coastal sites and a local fish market in jeddah, saudi arabia. bacterial counts of gills, skin, gut and muscle were examined on agar plates of macconkey's (mac), eosin methylene blue (emb) and thiosulfate citrate bile salts (tcbs) culture m ...201728855801
emergence of leuconostoc mesenteroides as a causative agent of oozing in carrots stored under non-ventilated conditions.long-term storage and transport of post-harvest carrots (daucus carota l.) require a low-temperature, high-relative-humidity environment, usually with low ventilation. following long-term storage, a slimy exudate (oozing) often appears on the carrots, leading to severe spoilage. we characterized the environmental conditions leading to these symptoms and identified the causative agent. simulation of non-ventilated storage conditions revealed accumulation of co2 (to 80%) and ethanol (to 1000 ppm); ...201728834204
serratia oryzae sp. nov., isolated from rice stems.a novel endophytic bacterium, strain j11-6t, was isolated from rice stems. its taxonomic position was investigated using a polyphasic approach. the novel strain was gram-staining-negative, facultatively anaerobic, motile and rod-shaped. although the results of phylogenetic analysis based on 16s rrna gene sequences indicated that j11-6t represented a member of the genus rahnella, multilocus sequence analysis (mlsa) on the basis of concatenated partial atpd, gyrb, rpob and infb gene sequences show ...201728853686
rahnella victoriana sp. nov., rahnella bruchi sp. nov., rahnella woolbedingensis sp. nov., classification of rahnella genomospecies 2 and 3 as rahnella variigena sp. nov. and rahnella inusitata sp. nov., respectively and emended description of the genus rahnella.isolations from oak symptomatic of acute oak decline, alder and walnut log tissue, and buprestid beetles in 2009-2012 yielded 32 gram-negative bacterial strains showing highest gyrb sequence similarity to rahnella aquatilis and ewingella americana. multilocus sequence analysis (using partial gyrb, rpob, infb and atpd gene sequences) delineated the strains into six mlsa groups. two mlsa groups contained reference strains of rahnella genomospecies 2 and 3, three groups clustered within the rahnell ...201425264035
chania multitudinisentens gen. nov., sp. nov., an n-acyl-homoserine-lactone-producing bacterium in the family enterobacteriaceae isolated from landfill site soil.phylogenetic and taxonomic characterization was performed for bacterium rb-25t, which was isolated from a soil sample collected in a former municipal landfill site in puchong, malaysia. growth occurred at 20-37 °c at ph 5-8 but not in the presence of 9 % (w/v) nacl or higher. the principal fatty acids were c16:0, c18:1ω7c and summed feature 3 (c16:1ω7c and/or iso-c15:0 2-oh). ubiquinone-8 was the only isoprenoid quinone detected. polar lipid analysis revealed the presence of phospholipid, phosph ...201626978486
native microflora in fresh-cut produce processing plants and their potentials for biofilm formation.representative food contact and nonfood contact surfaces in two mid-sized, fresh-cut processing facilities were sampled for microbiological analyses after routine daily sanitization. mesophilic and psychrotrophic bacteria on the sampled surfaces were isolated by plating on nonselective bacterial media. alternatively, bacteria were isolated after an incubation period that allowed the formation of heterogeneous biofilms on stainless steel beads. of over 1,000 tested isolates, most were capable of ...201323643124
nitrogen-fixing and uricolytic bacteria associated with the gut of dendroctonus rhizophagus and dendroctonus valens (curculionidae: scolytinae).the bark beetles of the genus dendroctonus feed on phloem that is a nitrogen-limited source. nitrogen fixation and nitrogen recycling may compensate or alleviate such a limitation, and beetle-associated bacteria capable of such processes were identified. raoultella terrigena, a diazotrophic bacteria present in the gut of dendroctonus rhizophagus and d. valens, exhibited high acetylene reduction activity in vitro with different carbon sources, and its nifh and nifd genes were sequenced. bacteria ...201323525792
rahnella aquatilis sepsis in a premature newborn.rahnella aquatilis is an infrequently isolated gram-negative rod within the enterobacteriaceae family. the organism's natural habitat is water. the organism is rarely isolated from clinical specimens and it seldom causes infection in immunocompetent individuals. here we present a one-month-old boy who was born prematurely at 27th week of gestation by cesarean section with a birth weight of 730 g. he developed sepsis caused by rahnella aquatilis during the treatment for ventilator associated pneu ...201526090257
interactions of three soil bacteria species with phyllosilicate surfaces in hybrid silica gels.to simulate iron consumption in soils, iron leaching from silicate minerals due to three heterotrophic bacterial strains and a chemical treatment was studied using hybrid silica gel (hsg) doped with two phyllosilicates, nontronite (nau-2) or low-iron-content montmorillonite (swy-2). hsg methodology, a novel way of separating bacteria cells from a colloidal mineral source, consisted in embedding colloidal mineral particles into an amorphous porous silica matrix using a classical sol-gel procedure ...201424628667
the spoilage characteristics of brochothrix thermosphacta and two psychrotolerant enterobacteriacae in vacuum packed lamb and the comparison between high and low ph cuts.the spoilage potential of brochothrix thermosphacta, serratia proteamaculans and rahnella aquatilis was investigated in vacuum packaged high (5.9 to 6.4) and low (5.4 to 5.8) ph lamb. vacuum packaged fore shank (m. extensor carpi radialis) and striploins (m. longissimus dorsi) (n=306) inoculated with ~100 cfu of individual bacteria were stored for twelve weeks at temperatures -1.5, 0, 2 and 7°c. spoilage characteristics and bacterial numbers were recorded and analysed in comparison to un-inocula ...201424548927
bioweathering of nontronite colloids in hybrid silica gel: implications for iron mobilization.this study aimed to study biotic iron dissolution using a new hybrid material constituted of well-dispersed mineral colloids in a silica gel matrix. this permitted to prevent adsorption of colloidal mineral particles on bacteria. hybrid silica gel (hsg) permitted to study bioweathering mechanisms by diffusing molecules.201424118978
exploring metal effects and synergistic interactions of ferric stimulation on azo-dye decolorization by new indigenous acinetobacter guillouiae ax-9 and rahnella aquatilis dx2b.the first-attempt study deciphered metal-interacting effects on dye-decolorizing capabilities of indigenous bioelectricity-generating strains, acinetobacter guillouiae ax-9 and rahnella aquatilis dx2b. most of the metallic ions were inhibitory to color removal capabilities of these strains. however, with supplementation of 5 mm ferric chloride, specific decolorization rate (sdr) of ax-9 increased by 55.48% compared to fe(3+)-free conditions. in contrast, sdr of dx2b decreased 75.35% due to the i ...201423743733
draft genome sequence of rahnella aquatilis strain hx2, a plant growth-promoting rhizobacterium isolated from vineyard soil in beijing, china.rahnella aquatilis strain hx2 is a plant growth-promoting, disease-suppressive rhizobacterium that was isolated from a vineyard soil in beijing, china. here, we report the genome sequence of this strain, which provides a valuable resource for future research examining the mechanisms of traits associated with plant growth promotion and biocontrol.201223144397
complete genome sequence of rahnella aquatilis cip 78.65.rahnella aquatilis cip 78.65 is a gammaproteobacterium isolated from a drinking water source in lille, france. here we report the complete genome sequence of rahnella aquatilis cip 78.65, the type strain of r. aquatilis.201222582378
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