Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| isolation of some pathogenic bacteria from the great spruce bark beetle, dendroctonus micans and its specific predator, rhizophagus grandis. | some bacteria were isolated from dendroctonus micans and its specific predator, rhizophagus grandis. six bacteria from d. micans were identified as bacillus pumilus, enterobacter intermedius, citrobacter freundii, cellulomonas flavigena, microbacterium liquefaciens and enterobacter amnigenus, three bacteria from r. grandis as klebsiella pneumoniae, pantoea agglomerans and serratia grimesii, on the basis of fatty acid methyl ester analysis and carbon utilization profile by using microbial identif ... | 2010 | 20336502 |
| size-resolved culturable airborne bacteria sampled in rice field, sanitary landfill, and waste incineration sites. | size-resolved bacterial concentrations in atmospheric aerosols sampled by using a six stage viable impactor at rice field, sanitary landfill, and waste incinerator sites were determined. culture-based and polymerase chain reaction (pcr) methods were used to identify the airborne bacteria. the culturable bacteria concentration in total suspended particles (tsp) was found to be the highest (848 colony forming unit (cfu)/m(3)) at the sanitary landfill sampling site, while the rice field sampling si ... | 2010 | 20623053 |
| evaluation of microbial dynamics during the ripening of a traditional taiwanese naturally fermented ham. | isolation and identification of the autochthonous starter from a naturally fermented meat allows control of the fermentation process and promises microbiological safety for this specialty. thus the purpose of this study was to identify the lactic acid bacteria and coagulase-negative cocci present in a traditional taiwanese naturally fermented ham (tnfh) and to study the microbial dynamics at different ripening stages; the approach was a combination of conventional microbiological cultivation, po ... | 2010 | 20417394 |
| selection of candidate probionts by two different screening strategies from atlantic cod (gadus morhua l.) larvae. | two primary selection criteria were used to collect a pool of nearly 500 candidate probiotic bacteria from atlantic cod (gadus morhua l.) larvae, i.e. the dominant intestinal bacterial flora and isolates with antagonistic activity against vibrio anguillarum. bacteria were isolated from cod larvae from five rearing groups with variable rearing technologies. the bacteria were brought to pure culture and characterized phenotypically. based on properties such as uniqueness, dominance and fermentativ ... | 2010 | 20097491 |
| culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot. | microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ... | 2010 | 20657618 |
| symbiotic relationship between microbacterium sp. sk0812 and candida tropicalis sk090404. | a bacterium growing inside yeast cytoplasm was observed by light microscope without staining. the bacterium was separately stained from yeast cell by a fluorescent dye, 4',6-diamidino-2-phenylindole (dapi). the bacterium actively moved inside yeast cytoplasm and propagated in company with the yeast growth. the bacterium was separated from the yeast cytoplasm by selective disruption of yeast cells and the yeast without the intracellular bacterium (ywob) was obtained by selective inactivation of b ... | 2010 | 20127466 |
| identities of epilithic hydrocarbon-utilizing diazotrophic bacteria from the arabian gulf coasts, and their potential for oil bioremediation without nitrogen supplementation. | gravel particles from four sites along the arabian gulf coast in autumn, winter, and spring were naturally colonized with microbial consortia containing between 7 and 400 × 10(2) cm(-2) of cultivable oil-utilizing bacteria. the 16s rrna gene sequences of 70 representatives of oil-utilizing bacteria revealed that they were predominantly affiliated with the gammaproteobacteria and the actinobacteria. the gammaproteobacteria comprised among others, the genera pseudomonas, pseudoalteromonas, shewane ... | 2010 | 20567813 |
| permeabilization of microbacterium oxylans shifts the conversion of puerarin from puerarin-7-o-glucoside to puerarin-7-o-fructoside. | the main product of the conversion of puerarin by unpermeabilized cells of bacterium microbacterium oxydans cgmcc 1788 was puerarin-7-o-glucoside (241 +/- 31.9 microm). permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 +/- 48.0 microm) which was identified as a novel puerarin-7-o-fructoside by electrospray ionization time-of-flight ms, (13)c nmr, (1)h nmr, and gc-ms analysis of ... | 2010 | 19943046 |
| microbacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. | a gram-stain-positive, aerobic, rod-shaped, yellow actinobacterium, designated mna2(t), was isolated from a hexachlorocyclohexane-contaminated soil in north india. strain mna2(t) showed 95 % 16s rrna gene sequence similarity with microbacterium halotolerans yim 70130(t). phylogenetic analysis of 16s rrna gene sequences showed that strain mna2(t) belonged to a clade represented by the genus microbacterium of the family microbacteriaceae. strain mna2(t) contained anteiso-c(15 : 0), anteiso-c(17 : ... | 2010 | 20023059 |
| microbacterium radiodurans sp. nov., a uv radiation-resistant bacterium isolated from soil. | strain gimn 1.002(t), a uv radiation-tolerant bacterium, was isolated from the upper sand layers of the gobi desert, xinjiang, china and characterized in order to determine its taxonomic position. cells were gram-reaction-positive, heterotrophic, strictly aerobic, short rods. 16s rrna gene sequence analysis revealed that strain gimn 1.002(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens dsm 20754(t) (98.8 % 16s rrna gene sequence similarity) and micro ... | 2010 | 20061506 |
| microbacterium ginsengiterrae sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field. | strain dcy37(t) was isolated from a soil sample of a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-staining-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain dcy37(t) belongs to the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium aerolatum dsm 14217(t) (98.8 %), microbacterium hydrocarbonoxydans d ... | 2010 | 20081025 |
| effect of bacterial cell size on electricity generation in a single-compartmented microbial fuel cell. | a single-compartmented microbial fuel cell composed of a graphite felt anode modified with neutral red (nr-anode) and a porous fe(ii)-carbon cathode (fec-cathode) were compared for electricity generation from microbacterium sp. and pseudomonas sp. under identical conditions. pseudomonas sp. was more than four times the size of microbacterium sp. based on sem images. in cyclic voltammetry, the redox reaction between microbacterium sp and electrode was three times the rate observed between pseudom ... | 2010 | 20013300 |
| molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques. | polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ... | 2010 | 20018377 |
| polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent. | biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ... | 2010 | 20093025 |
| microbacterium azadirachtae sp. nov., a plant-growth-promoting actinobacterium isolated from the rhizoplane of neem seedlings. | microbacterium strain ai-s262(t) was isolated from the rhizoplane of neem seedlings in the botanical garden of tamilnadu agricultural university, coimbatore, india, and subjected to phenotypic, chemotaxonomic and genetic characterization. cells of this strain were gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. strain ai-s262(t) contained mk-12 and mk-13 as the main respiratory quinones, anteiso-c(15 : 0), anteiso-c(17 : 0) ... | 2010 | 19734284 |
| microbacterium soli sp. nov., an alpha-glucosidase-producing bacterium isolated from soil of a ginseng field. | five gram-type-positive, aerobic, rod-shaped, non-motile strains of microbacterium (dcy 17(t), ms1, ms2, ms3 and ms4) were isolated from soil from a ginseng field in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, these strains were shown to be related to microbacterium esteraromaticum dsm 8609(t) (96.1 %), m. xylanilyticum dsm 16914(t) (96.0 %), m. aquimaris js54-2(t) (95.6 %), m. insulae ds-66(t) (95.5 %), m. ketosireducens ifo 14548(t) (95.5 %) and m. arabinogalactano ... | 2010 | 19654351 |
| microbacterium agarici sp. nov., microbacterium humi sp. nov. and microbacterium pseudoresistens sp. nov., isolated from the base of the mushroom agaricus blazei. | three gram-positive, rod-shaped bacteria (strains cc-sbck-209( t), cc-12309(t) and cc-5209(t)) were isolated from the stalk of the edible mushroom agaricus blazei grown in the laboratory. 16s rrna gene sequence analysis indicated that all three isolates clearly belonged to the genus microbacterium. strains cc-sbck-209( t) and cc-12309(t) were most related closely to the type strain of microbacterium halotolerans (95.9 and 96.1 % 16s rrna gene sequence similarity, respectively). these two novel s ... | 2010 | 19661494 |
| characterization of the ars gene cluster from extremely arsenic-resistant microbacterium sp. strain a33. | the arsenic resistance gene cluster of microbacterium sp. a33 contains a novel pair of genes (arstx) encoding a thioredoxin system that are cotranscribed with an unusual arsrc2 fusion gene, acr3, and arsc1 in an operon divergent from arsc3. the whole ars gene cluster is required to complement an escherichia coli ars mutant. arsrc2 negatively regulates the expression of the pentacistronic operon. arsc1 and arsc3 are related to thioredoxin-dependent arsenate reductases; however, arsc3 lacks the tw ... | 2010 | 19966021 |
| isolation and characterization of beneficial bacteria associated with citrus roots in florida. | cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. citrus roots from huanglongbing (hlb) diseased symptomatic and asymptomatic citrus were used in this study. a total of 227 and 125 morphologically distinct colonies were isolated and characterized from hlb asymptomatic and symptomatic trees, respectively. we obs ... | 2011 | 21360139 |
| genome sequence of microbacterium testaceum stlb037, an n-acylhomoserine lactone-degrading bacterium isolated from potato leaves. | microbacterium testaceum is an endophytic gram-positive bacterium that resides within plant hosts. m. testaceum stlb037 was isolated from potato leaves and shows n-acylhomoserine lactone-degrading activity. here, we present the 3.98-mb complete genome sequence of stlb037, with an average gc content of 70.28%. | 2011 | 21357489 |
| bacterial flora as indicated by pcr-temperature gradient gel electrophoresis (tgge) of 16s rdna gene fragments from isolated guts of phlebotomine sand flies (diptera: psychodidae). | in this study, we tested the capacity of temperature gradient gel electrophoresis (tgge)-based fingerprinting of 16s rdna pcr fragments to assess bacterial composition in a single isolated sand fly gut. bacterial content was studied in different life stages of a laboratory-reared colony of phlebotomus duboscqi and in a wild-caught phlebotomus papatasi population. our study demonstrates that a major reorganization in the gut bacterial community occurs during metamorphosis of sand flies. chlorofle ... | 2011 | 21366767 |
| isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi. | soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ... | 2011 | 21219415 |
| exogenous coproporphyrin iii production by corynebacterium aurimucosum and microbacterium oxydans in erythrasma lesions. | erythrasma is a superficial skin disease caused by gram-positive corynebacterium species. coral-red fluorescence under wood's light, strongly suggestive of erythrasma, can be attributed to the presence of porphyrins. fractionated porphyrin analysis in erythrasma lesions is yet to be reported. we attempted to investigate erythrasma lesions by isolating the responsible bacteria and determining their exogenous porphyrin production by hplc analysis. we observed a 78-year-old woman with erythrasma wh ... | 2011 | 21393451 |
| characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses. | diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ... | 2011 | 20859703 |
| comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries. | the preparation of enantiopure tertiary alcohols is of great contemporary interest due to the application of these versatile building blocks in organic synthesis and as precursors towards high value pharmaceutical compounds. herein, we describe two approaches taken towards the discovery of novel biocatalysts for the synthesis of these valuable compounds. the first approach was initiated with screening of 47 bacterial strains for hydrolytic activity towards the simple tertiary alcohol ester tert- ... | 2011 | 21318363 |
| characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of herculaneum in italy. | microbiological characterization of frescos in four different locations (collegio degli augustali, casa del colonnato tuscanico, casa dello scheletro and casa del gran portale) of excavation sites of herculaneum was carried out. the use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. the microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming an ... | 2011 | 21882662 |
| Microbacterium murale sp. nov., isolated from an indoor wall. | A Gram-positive rod shaped bacterium (01-GI-001T) isolated from a wall colonized with moulds was studied for its taxonomic position. The 16S rRNA gene sequence analysis showed, that the isolate clearly grouped into the Microbacterium cluster. On the basis of pairwise comparisons of the 16S rRNA gene sequences, isolate 01-Gi-001T was most closely related to the type strains of Microbacterium hydrocarbonoxydans (98.8%), Microbacterium profundi (98.7%), Microbacterium phyllosphaerae (98.3%) and Mic ... | 2011 | 22199224 |
| changes in the bacterial community structure and diversity during bamboo retting. | microbial retting is a critical step in obtaining fiber bundles from bamboo culm using indigenous microorganisms. a cultivation-independent technique for monitoring the changes in bacteria community during bamboo retting was applied in this work. this technique involves genetic profiling of pcr-amplified small-subunit rrna and the single-strand conformation polymorphism (sscp) gel analysis of the pcr-amplified 16s rdna fragments. the study revealed that both the structure and the diversity of in ... | 2011 | 21695788 |
| characterization of a recombinant (-)γ-lactamase from microbacterium hydrocarbonoxydans. | a (-)γ-lactamase, mhg, from microbacterium hydrocarbonoxydans was over-expressed in e. coli and was characterized after purification. the maximum activity was at ph 8.0 and 60°c and the half life of mhg was ~30 min at 75°c. the enzyme was activated by dtt. the catalytic triad of the (-)γ-lactamase is comprised of residues ser98, asp230, and his259 and an oxyanion hole was formed by tyr32 and met99 according to the alignment results. under native conditions, the (-)γ-lactamase consists of two 31 ... | 2011 | 21968479 |
| the actinobacterium microbacterium sp. 16sh accepts pbbr1-based pprobe vectors, forms biofilms, invades roots, and fixes n(2) associated with micropropagated sugarcane plants. | members of the genus microbacterium lineage of gram-positive actinobacteria are increasingly being reported to display significant traits associated with environmental biotechnology and bioengineering. 16sh is a nitrogen-fixing bacterial strain isolated from a surface-sterilized stem of sugarcane grown in guangxi, china. analysis of 16s rrna gene sequences revealed that 16sh belonged to the genus microbacterium. pprobe-ptet(r) plasmids were constructed by cloning the promoter region of the tet ... | 2011 | 22002067 |
| L-Tryptophan Production by Auxotrophic and Analogue Resistant Mutants of Aureobacterium flavescens. | A number of tyrosine plus phenylalanine double auxotrophic mutants were isolated by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) treatment of a locally isolated strain of Aureobacterium flavescens of which 11A(39) and 11A(17) were selected on the basis of their tryptophan production in a mineral salt medium over other isolated mutant strains. The mutational block in the aromatic amino acid biosynthetic pathway of the selected double auxotrophs were determined. By controlling pH of the production m ... | 2011 | 22084602 |
| hessian fly-associated bacteria: transmission, essentiality, and composition. | plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ... | 2011 | 21858016 |
| biodegradation of phthalate esters in polluted soil by using organic amendment. | this study investigated the biodegradation of the phthalate esters (paes) di-n-butyl phthalate (dbp) and di-(2-ethyl hexyl) phthalate (dehp) in sludge and sludge-amended soil. dbp (100 mg kg(-1)) and dehp (100 mg kg(-1)) were added to sewage sludge, which was subsequently added to soil. the results showed that sewage sludge can degrade paes and the addition of sewage sludge to soil enhanced pae degradation. sludge samples were separated into fractions with various particle size ranges, which spa ... | 2011 | 21614716 |
| diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze). | the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ... | 2011 | 21715960 |
| modification of norfloxacin by a microbacterium sp. strain isolated from a wastewater treatment plant. | antimicrobial residues found in municipal wastewater may increase selective pressure on microorganisms for development of resistance, but studies with mixed microbial cultures derived from wastewater have suggested that some bacteria are able to inactivate fluoroquinolones. medium containing n-phenylpiperazine and inoculated with wastewater was used to enrich fluoroquinolone-modifying bacteria. one bacterial strain isolated from an enrichment culture was identified by 16s rrna gene sequence anal ... | 2011 | 21724893 |
| Microbacterium amylolyticum sp. nov., N5T a bacterium isolated from industrial waste soil. | A Gram-positive, heterotrophic, aerobic, non-motile, non-endospore forming, rod-shaped and yellow colored bacterium N5T was isolated from a soil sample collected from the industrial waste site in Noida at the outskirts of Delhi, India. Strain N5T showed highest 16S rRNA gene similarity to Microbacterium indicum LMG 23459T (97.59 %) and Microbacterium gubbeenense LMG 19263T (97.18 %). The 16S rRNA gene sequence similarity between strain N5T and the validly published name of the species of genus M ... | 2011 | 22039005 |
| induction and resuscitation of the viable but nonculturable state in a cyanobacteria-lysing bacterium isolated from cyanobacterial bloom. | the viable but nonculturable (vbnc) state has been found to be a growth strategy used by many aquatic pathogens; however, few studies have focused on vbnc state on other aquatic bacterial groups. the purpose of this study was to explore the vbnc state of cyanobacteria-lysing bacteria and the conditions that regulate their vbnc state transformation. three cyanobacteria-lysing heterotrophic bacterial strains (f1, f2 and f3) were isolated with liquid infection method from a lake that has experience ... | 2011 | 21850447 |
| Microbacterium suwonense sp. nov., isolated from cow dung. | An actinomycete strain, designated M1T8B9(T), was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17(T) (98.2%) and Microbacterium esteraromaticum DSM 8609(T) (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosp ... | 2011 | 22068506 |
| culturable bacterial communities on leaf sheaths and panicles of rice plants in japan. | culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ... | 2011 | 22081407 |
| Diagnostic value of an immunochromatographic test over clinical predictors for tuberculosis in HIV patients. | The value of an immunochromatographic test for tuberculosis (ICT-TB) combined with clinical predictors has yet to be evaluated in Thailand. This study aimed to assess any additional diagnostic value of an ICT-TB test over that of clinical predictors in a group of human immunodeficiency virus (HIV) patients as well as in subgroups of HIV patients classified by clinical risk scores. | 2011 | 22003307 |
| adsorption behavior of microbes on a qcm chip modified with an artificial siderophore-fe(3+) complex. | three hydroxamate-type artificial siderophores with terminal nh(2) groups, tris[2-{3-(n-acyl-n-hydroxamino)propylamido}propyl]aminomethane (1-3, acyl-r group = me, et, and ph, respectively), and their fe(3+) complexes, 4-6, were prepared. the stability constant (log β) of 4 was estimated to be about 31 by its edta titration. the biological activities of 4-6 for microbacterium flavescens, which is a hydroxamate-type siderophore, auxotrophic gram-positive microbe, clearly indicated that they perme ... | 2011 | 22182317 |
| [Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut]. | We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping. | 2011 | 21866710 |
| butyric acid- and dimethyl disulfide-assimilating microorganisms in a biofilter treating air emissions from a livestock facility. | biofiltration has proven an efficient tool for the elimination of volatile organic compounds (vocs) and ammonia from livestock facilities, thereby reducing nuisance odors and ammonia emissions to the local environment. the active microbial communities comprising these filter biofilms have not been well characterized. in this study, a trickle biofilter treating air from a pig facility was investigated and proved efficient in removing carboxylic acids (>70% reduction), mainly attributed to the pri ... | 2011 | 22003018 |
| biofouling characteristics and identification of preponderant bacteria at different nutrient levels in batch tests of a recirculating cooling water system. | understanding the influence of nutrient levels on biofouling control is an important requirement for management strategies in a recirculating cooling water system. nutrient limitation may be one way to control biofouling development without increasing biocide dosing. therefore, this study was carried out to investigate the effects of nutrient levels on biofouling characteristics and to identify the preponderant bacteria in the batch tests with a simulated cooling water system. the biofouling cha ... | 2011 | 21879565 |
| isolation and characterization of metal resistant-tolerant rhizosphere bacteria from the serpentine soils in turkey. | despite the number of studies describing metal hyper-accumulating plants and their associated bacteria in various regions and countries, there is no information on rhizosphere microbial potential of the turkish serpentine soils. this study aimed to explore the rhizosphere microbial diversity of ni-resistant, hyper-accumulating plants grown on ni-rich soils and their metal tolerance-resistance characteristics. one hundred ninety-one locations were visited to collect soil and plant samples from di ... | 2011 | 21404012 |
| diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan. | a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ... | 2011 | 21487197 |
| degradation of cyhalofop-butyl (cyb) by pseudomonas azotoformans strain qdz-1 and cloning of a novel gene encoding cyb-hydrolyzing esterase. | cyhalofop-butyl (cyb) is a widely used aryloxyphenoxy propanoate (aopp) herbicide for control of grasses in rice fields. five cyb-degrading strains were isolated from rice field soil and identified as agromyces sp., stenotrophomonas sp., aquamicrobium sp., microbacterium sp., and pseudomonas azotoformans ; the results revealed high biodiversity of cyb-degrading bacteria in rice soil. one strain, p. azotoformans qdz-1, degraded 84.5% of 100 mg l(-1) cyb in 5 days of incubation in a flask and util ... | 2011 | 21534595 |
| production of fusarium verticillioides biocontrol agents, bacillus amyloliquefaciens and microbacterium oleovorans, using different growth media: evaluation of biomass and viability after freeze-drying. | the aims of this study were to compare the viability and biomass production of b. amyloliquefaciens and m. oleovorans in different growth media, and the efficiency of a freeze-drying method as a possible formulation process. b. amyloliquefaciens and m. oleovorans were grown in 100 ml of four different media. media water activity was modified at 0.99, 0.98, 0.97 and 0.96. nutrient yeast dextrose broth (nydb) and molasses soy powder (msb) media were selected and survival levels of cells were deter ... | 2011 | 21547798 |
| microbacterium yannicii sp. nov., isolated from arabidopsis thaliana roots. | strain g72t was isolated from surface-sterilized roots of arabidopsis thaliana growing in its natural habitat. this gram-positive, rod-shaped, non-motile, microaerophilic and aerobically growing isolate was characterized in a polyphasic approach. on the basis of the 16s rrna gene sequence similarity, strain g72t belongs to the genus microbacterium. however reassociation values in a dna-dna hybridization analysis with closely related strains were between 42.1% and 13.5%. the dna g+c content was 7 ... | 2011 | 21571929 |
| microbial degradation of microcystin in florida's freshwaters. | presence of microcystin (mc), a predominant freshwater algal toxin and a suspected liver carcinogen, in florida's freshwaters poses serious health threat to humans and aquatic species. being recalcitrant to conventional physical and chemical water treatment methods, biological methods of mc removal is widely researched. water samples collected from five sites of lake okeechobee (lo) frequently exposed to toxic microcystis blooms were used as inoculum for enrichment with microcystin lr (mc-lr) su ... | 2011 | 21611743 |
| culturable bacterial community analysis in the root domains of two different tree peony (paeonia ostii) variety plants. | a total of 985 bacterial strains with different colony characteristics were isolated from the root of tree peony plants (variety 'fengdan' and 'lan furong'), which were 69 otus identified by ardra. representatives of each group were selected for partial 16s rrna gene sequencing and phylogenetic analysis. the major group in the bulk soil, rhizosphere and rhizoplane of 'fengdan' were firmicutes (63.2%), actinobacteria (36.3%) and betaproteobacteria (53.0%), respectively. while the major bacteria g ... | 2011 | 21623895 |
| culture-dependent and -independent molecular analysis of the bacterial community within uranium ore. | the bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16s rrna genes. the major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. together with near neutral ph, moderate organic carbon content, elevated u and other heavy metals (v, ni, mn, cu, etc.), the ore showed high microbial count ... | 2011 | 21656801 |
| microbacterium immunditiarum sp. nov., a novel actinobacterium isolated from a landfill surface soil. | a gram-positive, non-endospore forming bacterium designated as strain sk 18t was isolated from surface soil of a landfill by dilution plating on tryptic soy broth agar (tsba). preliminary characterization of strain sk 18t using biochemical tests, fame analysis and partial 16s rrna gene sequencing placed it within the genus microbacterium. analysis of the cell wall indicated that the peptidoglycan was of the b-type of cross-linkage containing amino acids lysine and ornithine with muramic acid in ... | 2011 | 22058326 |
| phylogenetic perspectives of nitrogen-fixing actinobacteria. | it was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ... | 2011 | 21779790 |
| structure and mechanism of iron translocation by a dps protein from microbacterium arborescens. | dps enzymes are a major class of dodecameric proteins which bacteria use to detoxify their cytosol through the uptake of reactive iron species. in the stationary growth phase of bacteria, dps enzymes (dna protection during starvation) are primarily used to protect dna by bio-crystallization. to characterize the wildtype dps protein from microbacterium arborescens, which displays additional catalytic functions (amide hydrolysis and synthesis), we determined the crystal structure to a resolution o ... | 2011 | 21768097 |
| isolation and characterization of lead-tolerant ochrobactrum intermedium and its role in enhancing lead accumulation by eucalyptus camaldulensis. | in this study, the potential of rhizospheric bacteria in promoting the growth and pb accumulation by the woody plant eucalyptus camaldulensis under hydroponic conditions was investigated for the first time. three pb-tolerant bacteria were isolated from the rhizosphere of e. camaldulensis grown in pb-contaminated soils in the bo ngam pb mine, thailand. based on analysis of partial 16s rrna gene sequence, the three isolates were identified as microbacterium paraoxydans bn-2, ochrobactrum intermedi ... | 2011 | 21764101 |
| enzymatic degradation of granular potato starch by microbacterium aurum strain b8.a. | microbacterium aurum strain b8.a was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. extracellular enzymes hydrolyzing granular starch were detected in the growth medium of m. aurum b8.a, while the type strain m. aurum dsmz 8600 produced very little amylase activity, and hence was unable to degrade granular starch. the strain b8.a extracellular enzyme fraction degraded wheat, tapioca and potato starch ... | 2011 | 21732245 |
| High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs. | High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase-amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. ... | 2011 | 22112948 |
| interactions between various microbes and ginseng botanicals. | three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ... | 2011 | 21254831 |
| molecular analysis of autochthonous microbiota along the digestive tract of juvenile grouper epinephelus coioides following probiotic bacillus pumilus administration. | aims: to evaluate the diversity of dominant autochthonous microbiota along the digestive tract of juvenile epinephelus coioides following the dietary administration of probiotic bacillus pumilus for 60 days. methods and results: polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) with subsequently sequencing analysis was used to assess the gut microbiota. generally similar dgge patterns were observed in the foregut, midgut and hindgut of e. coioides, while the similari ... | 2011 | 21294823 |
| facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium. | we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ... | 2011 | 21374057 |
| microbacterium mitrae sp. nov., isolated from salted turban shell. | a novel bacterium (strain m4-8(t)) belonging to the genus microbacterium was isolated from salted turban shell, which is a traditional fermented food in korea. its morphology, physiology, biochemical features and 16s rrna gene sequence were characterized. cells of this strain were gram-positive, non-motile, non-spore-forming rods that formed yellow-pigmented colonies. it grew in 0-8 % (w/v) nacl and at 15-37 °c, with optimal growth occurring in 1 % (w/v) nacl and at 30 °c. phylogenetic analysis ... | 2011 | 20305059 |
| the potential of epiphytic hydrocarbon-utilizing bacteria on legume leaves for attenuation of atmospheric hydrocarbon pollutants. | the leaves of two legumes, peas and beans, harbored on their surfaces up to 9×10⁷ cells g⁻¹ of oil-utilizing bacteria. less numbers, up to 5×10⁵ cells g⁻¹ inhabited leaves of two nonlegume crops, namely tomato and sunflower. older leaves accommodated more of such bacteria than younger ones. plants raised in oily environments were colonized by much more oil-utilizing bacteria than those raised in pristine (oil-free) environments. similar numbers were counted on the same media in which nitrogen sa ... | 2012 | 22054577 |
| Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. | Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ... | 2012 | 22130193 |
| enzymatic biotransformation of ginsenoside rb1 to 20(s)-rg3 by recombinant β-glucosidase from microbacterium esteraromaticum. | microbacterium esteraromaticum was isolated from ginseng field. the β-glucosidase gene (bgp1) from m. esteraromaticum was cloned and expressed in escherichia coli bl21 (de3). the bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. the recombinant β-glucosidase enzyme (bgp1) was purified and characterized. the molecular mass of purified bgp1 was 87.5 kda, as determined by sds-page. using 0.1 mg ml(-1) enzyme in 20 mm sodiu ... | 2012 | 22249721 |
| High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol. | Stable-isotope probing (SIP) has proved a valuable cultivation-independent tool for linking specific microbial populations to selected functions in various natural and engineered systems. However, application of SIP to microbial populations with relatively minor buoyant density increases, such as populations that utilize compounds as a nitrogen source, results in reduced resolution of labeled populations. We therefore developed a tandem quantitative PCR (qPCR)-TRFLP (terminal restriction fragmen ... | 2012 | 22038597 |
| identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis candidates. | to describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ... | 2012 | 22074685 |