PMID(sorted ascending)
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
size-resolved culturable airborne bacteria sampled in rice field, sanitary landfill, and waste incineration sites.size-resolved bacterial concentrations in atmospheric aerosols sampled by using a six stage viable impactor at rice field, sanitary landfill, and waste incinerator sites were determined. culture-based and polymerase chain reaction (pcr) methods were used to identify the airborne bacteria. the culturable bacteria concentration in total suspended particles (tsp) was found to be the highest (848 colony forming unit (cfu)/m(3)) at the sanitary landfill sampling site, while the rice field sampling si ...201020623053
impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against fusarium verticillioides.bacillus amyloliquefaciens and microbacterium oleovorans reduced the fusarium verticillioides count and significantly decreased fumonisin b(1) and b(2) levels in maize grains. the aim of this study was to determine the effect of water stress tolerance and heat shock survival upon cells of the biocontrol agents b. amyloliquefaciens and m. oleovorans. the a(w) of solid and liquid media and tryptic soy medium was modified to 0.99, 0.98, 0.97 and 0.96 by addition of ionic solute nacl and non-ionic s ...201020624459
microbacterium arthrosphaerae sp. nov., isolated from the faeces of the pill millipede arthrosphaera magna attems.a gram-reaction-positive, rod-shaped bacterium, designated strain cc-vm-y(t), was isolated from the faeces of the pill millipede arthrosphaera magna attems from india and was subsequently studied to determine its taxonomic position. based on16s rrna gene sequence similarities, the isolate clearly grouped with members of the genus microbacterium. on the basis of pairwise comparisons of the 16s rrna gene sequences, strain cc-vm-y(t) was most closely related to microbacterium insulae ds-66(t) (98 % ...201020656817
culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot.microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ...201020657618
long-term hg pollution-induced structural shifts of bacterial community in the terrestrial isopod (porcellio scaber) previous studies we detected lower species richness and lower hg sensitivity of the bacteria present in egested guts of porcellio scaber (crustacea, isopoda) from chronically hg polluted than from unpolluted environment. basis for such results were further investigated by sequencing of 16s rrna genes of mercury-resistant (hgr) isolates and clone libraries. we observed up to 385 times higher numbers of hgr bacteria in guts of animals from polluted than from unpolluted environment. the majority ...201020724045
isolation and characterization of hexavalent chromium-reducing rhizospheric bacteria from a wetland.scirpus americanus pers. occurs naturally in "san germán," a pond that serves as a receptor of industrial wastewater in guanajuato, méxico. this plant accumulates metals mainly in the root: concentrations (mg/kg) of cr, as, cd and se were 970, 49, 41, and 85 respectively. analysis of rhizosphere samples indicated bacterial population of 10(8) cfu g(-1) in media with 0.2 mm cr(vi) and 10 mm sodium gluconate. thirteen isolates were obtained and phylogenetic analyses (16s rrna) indicated they corre ...201020734910
characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses.diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ...201120859703
comparison of maldi tof with conventional identification of clinically relevant bacteria.a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory.201020924806
diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania.aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ...201020941581
glycosylation genes expressed in seam cells determine complex surface properties and bacterial adhesion to the cuticle of caenorhabditis elegans.the surface of the nematode caenorhabditis elegans is poorly understood but critical for its interactions with the environment and with pathogens. we show here that six genes (bus-2, bus-4, and bus-12, together with the previously cloned srf-3, bus-8, and bus-17) encode proteins predicted to act in surface glycosylation, thereby affecting disease susceptibility, locomotory competence, and sexual recognition. mutations in all six genes cause resistance to the bacterial pathogen microbacterium nem ...201020980242
comparative bioremediation potential of four rhizospheric microbial species against lindane.four microbial species (kocuria rhizophila, microbacterium resistens, staphylococcus equorum and staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a lindane contaminated environment and acclimatized in lindane spiked media (5-100 μg ml⁻¹). the isolated species were inoculated with soil containing 5, 50 and 100 mg kg⁻¹ of lindane and incubated at room temperature. soil samples were collected periodically to evaluate the microbial ...201021044795
root exudates modify bacterial diversity of phenanthrene degraders in pah-polluted soil but not phenanthrene degradation determine whether the diversity of phenanthrene-degrading bacteria in an aged polycyclic aromatic hydrocarbon (pah) contaminated soil is affected by the addition of plant root exudates, dna stable isotope probing (sip) was used. microcosms of soil with and without addition of ryegrass exudates and with ¹³c-labelled phenanthrene (phe) were monitored over 12 days. phe degradation was slightly delayed in the presence of added exudate after 4 days of incubation. after 12 days, 68% of added phe di ...201021087382
description of a novel actinobacterium microbacterium assamensis sp. nov., isolated from water sample collected from the river brahmaputra, assam, india.a gram-positive, yellow pigmented actinobacterium, strain s2-48(t) was isolated from water sample collected from the river brahmaputra, assam, india and subjected to a polyphasic taxonomic study. most of the physiological and biochemical properties, major fatty acids (c(15:0) anteiso, iso c(16:0) and c(17:0) anteiso), estimated dna g+c content (70.2 mol%) and 16s rrna gene sequence analysis showed that strain s2-48(t) belonged to the genus microbacterium. strain s2-48(t) exhibited highest 16s rr ...201021104080
lactose-enhanced cellulase production by microbacterium sp. isolated from fecal matter of zebra (equus zebra).a cellulase-producing bacterial strain designated z5 was isolated from the fecal matter of zebra (equus zebra). the strain was identified as microbacterium sp. on the basis of 16s rdna sequence analysis. the effect of substrates like cmc, avicel, starch, maltose, sucrose, glucose, fructose, galactose, and lactose on cellulase production was also determined. lactose as the sole carbon source induced cellulase production in this bacterial strain and a positive synergistic effect of lactose and cmc ...201021107842
peritonitis due to microbacterium sp in a patient on cycler peritoneal dialysis. 201021148068
diversity of bacterial endophytes in roots of mexican husk tomato plants (physalis ixocarpa) and their detection in the rhizosphere.endophytic bacterial diversity was estimated in mexican husk tomato plant roots by amplified rdna restriction analysis and sequence homology comparison of the 16s rdna genes. sixteen operational taxonomic units from the 16s rdna root library were identified based on sequence analysis, including the classes gammaproteobacteria, betaproteobacteria, actinobacteria, and bacilli. the predominant genera were stenotrophomonas (21.9%), microbacterium (17.1%), burkholderia (14.3%), bacillus (14.3%), and ...201021157706
central venous catheter-related bacteraemia due to microbacterium paraoxydans in a patient with no significant immunodeficiency. 201021169279
the alternating access mechanism of transport as observed in the sodium-hydantoin transporter mhp1.secondary active transporters move molecules across cell membranes by coupling this process to the energetically favourable downhill movement of ions or protons along an electrochemical gradient. they function by the alternating access model of transport in which, through conformational changes, the substrate binding site alternately faces either side of the membrane. owing to the difficulties in obtaining the crystal structure of a single transporter in different conformational states, relative ...201021169684
isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi.soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ...201121219415
interactions between various microbes and ginseng botanicals.three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ...201121254831
[diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province].to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china.201021268889
molecular analysis of autochthonous microbiota along the digestive tract of juvenile grouper epinephelus coioides following probiotic bacillus pumilus administration.aims: to evaluate the diversity of dominant autochthonous microbiota along the digestive tract of juvenile epinephelus coioides following the dietary administration of probiotic bacillus pumilus for 60 days. methods and results: polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) with subsequently sequencing analysis was used to assess the gut microbiota. generally similar dgge patterns were observed in the foregut, midgut and hindgut of e. coioides, while the similari ...201121294823
comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries.the preparation of enantiopure tertiary alcohols is of great contemporary interest due to the application of these versatile building blocks in organic synthesis and as precursors towards high value pharmaceutical compounds. herein, we describe two approaches taken towards the discovery of novel biocatalysts for the synthesis of these valuable compounds. the first approach was initiated with screening of 47 bacterial strains for hydrolytic activity towards the simple tertiary alcohol ester tert- ...201121318363
genome sequence of microbacterium testaceum stlb037, an n-acylhomoserine lactone-degrading bacterium isolated from potato leaves.microbacterium testaceum is an endophytic gram-positive bacterium that resides within plant hosts. m. testaceum stlb037 was isolated from potato leaves and shows n-acylhomoserine lactone-degrading activity. here, we present the 3.98-mb complete genome sequence of stlb037, with an average gc content of 70.28%.201121357489
isolation and characterization of beneficial bacteria associated with citrus roots in florida.cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. citrus roots from huanglongbing (hlb) diseased symptomatic and asymptomatic citrus were used in this study. a total of 227 and 125 morphologically distinct colonies were isolated and characterized from hlb asymptomatic and symptomatic trees, respectively. we obs ...201121360139
bacterial flora as indicated by pcr-temperature gradient gel electrophoresis (tgge) of 16s rdna gene fragments from isolated guts of phlebotomine sand flies (diptera: psychodidae).in this study, we tested the capacity of temperature gradient gel electrophoresis (tgge)-based fingerprinting of 16s rdna pcr fragments to assess bacterial composition in a single isolated sand fly gut. bacterial content was studied in different life stages of a laboratory-reared colony of phlebotomus duboscqi and in a wild-caught phlebotomus papatasi population. our study demonstrates that a major reorganization in the gut bacterial community occurs during metamorphosis of sand flies. chlorofle ...201121366767
facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium.we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ...201121374057
exogenous coproporphyrin iii production by corynebacterium aurimucosum and microbacterium oxydans in erythrasma lesions.erythrasma is a superficial skin disease caused by gram-positive corynebacterium species. coral-red fluorescence under wood's light, strongly suggestive of erythrasma, can be attributed to the presence of porphyrins. fractionated porphyrin analysis in erythrasma lesions is yet to be reported. we attempted to investigate erythrasma lesions by isolating the responsible bacteria and determining their exogenous porphyrin production by hplc analysis. we observed a 78-year-old woman with erythrasma wh ...201121393451
isolation and characterization of metal resistant-tolerant rhizosphere bacteria from the serpentine soils in turkey.despite the number of studies describing metal hyper-accumulating plants and their associated bacteria in various regions and countries, there is no information on rhizosphere microbial potential of the turkish serpentine soils. this study aimed to explore the rhizosphere microbial diversity of ni-resistant, hyper-accumulating plants grown on ni-rich soils and their metal tolerance-resistance characteristics. one hundred ninety-one locations were visited to collect soil and plant samples from di ...201121404012
diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan.a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ...201121487197
degradation of cyhalofop-butyl (cyb) by pseudomonas azotoformans strain qdz-1 and cloning of a novel gene encoding cyb-hydrolyzing esterase.cyhalofop-butyl (cyb) is a widely used aryloxyphenoxy propanoate (aopp) herbicide for control of grasses in rice fields. five cyb-degrading strains were isolated from rice field soil and identified as agromyces sp., stenotrophomonas sp., aquamicrobium sp., microbacterium sp., and pseudomonas azotoformans ; the results revealed high biodiversity of cyb-degrading bacteria in rice soil. one strain, p. azotoformans qdz-1, degraded 84.5% of 100 mg l(-1) cyb in 5 days of incubation in a flask and util ...201121534595
production of fusarium verticillioides biocontrol agents, bacillus amyloliquefaciens and microbacterium oleovorans, using different growth media: evaluation of biomass and viability after freeze-drying.the aims of this study were to compare the viability and biomass production of b. amyloliquefaciens and m. oleovorans in different growth media, and the efficiency of a freeze-drying method as a possible formulation process. b. amyloliquefaciens and m. oleovorans were grown in 100 ml of four different media. media water activity was modified at 0.99, 0.98, 0.97 and 0.96. nutrient yeast dextrose broth (nydb) and molasses soy powder (msb) media were selected and survival levels of cells were deter ...201121547798
microbacterium yannicii sp. nov., isolated from arabidopsis thaliana roots.strain g72t was isolated from surface-sterilized roots of arabidopsis thaliana growing in its natural habitat. this gram-positive, rod-shaped, non-motile, microaerophilic and aerobically growing isolate was characterized in a polyphasic approach. on the basis of the 16s rrna gene sequence similarity, strain g72t belongs to the genus microbacterium. however reassociation values in a dna-dna hybridization analysis with closely related strains were between 42.1% and 13.5%. the dna g+c content was 7 ...201121571929
microbial degradation of microcystin in florida's freshwaters.presence of microcystin (mc), a predominant freshwater algal toxin and a suspected liver carcinogen, in florida's freshwaters poses serious health threat to humans and aquatic species. being recalcitrant to conventional physical and chemical water treatment methods, biological methods of mc removal is widely researched. water samples collected from five sites of lake okeechobee (lo) frequently exposed to toxic microcystis blooms were used as inoculum for enrichment with microcystin lr (mc-lr) su ...201121611743
biodegradation of phthalate esters in polluted soil by using organic amendment.this study investigated the biodegradation of the phthalate esters (paes) di-n-butyl phthalate (dbp) and di-(2-ethyl hexyl) phthalate (dehp) in sludge and sludge-amended soil. dbp (100 mg kg(-1)) and dehp (100 mg kg(-1)) were added to sewage sludge, which was subsequently added to soil. the results showed that sewage sludge can degrade paes and the addition of sewage sludge to soil enhanced pae degradation. sludge samples were separated into fractions with various particle size ranges, which spa ...201121614716
culturable bacterial community analysis in the root domains of two different tree peony (paeonia ostii) variety plants.a total of 985 bacterial strains with different colony characteristics were isolated from the root of tree peony plants (variety 'fengdan' and 'lan furong'), which were 69 otus identified by ardra. representatives of each group were selected for partial 16s rrna gene sequencing and phylogenetic analysis. the major group in the bulk soil, rhizosphere and rhizoplane of 'fengdan' were firmicutes (63.2%), actinobacteria (36.3%) and betaproteobacteria (53.0%), respectively. while the major bacteria g ...201121623895
culture-dependent and -independent molecular analysis of the bacterial community within uranium ore.the bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16s rrna genes. the major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. together with near neutral ph, moderate organic carbon content, elevated u and other heavy metals (v, ni, mn, cu, etc.), the ore showed high microbial count ...201121656801
changes in the bacterial community structure and diversity during bamboo retting.microbial retting is a critical step in obtaining fiber bundles from bamboo culm using indigenous microorganisms. a cultivation-independent technique for monitoring the changes in bacteria community during bamboo retting was applied in this work. this technique involves genetic profiling of pcr-amplified small-subunit rrna and the single-strand conformation polymorphism (sscp) gel analysis of the pcr-amplified 16s rdna fragments. the study revealed that both the structure and the diversity of in ...201121695788
diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze).the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ...201121715960
modification of norfloxacin by a microbacterium sp. strain isolated from a wastewater treatment plant.antimicrobial residues found in municipal wastewater may increase selective pressure on microorganisms for development of resistance, but studies with mixed microbial cultures derived from wastewater have suggested that some bacteria are able to inactivate fluoroquinolones. medium containing n-phenylpiperazine and inoculated with wastewater was used to enrich fluoroquinolone-modifying bacteria. one bacterial strain isolated from an enrichment culture was identified by 16s rrna gene sequence anal ...201121724893
enzymatic degradation of granular potato starch by microbacterium aurum strain b8.a.microbacterium aurum strain b8.a was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. extracellular enzymes hydrolyzing granular starch were detected in the growth medium of m. aurum b8.a, while the type strain m. aurum dsmz 8600 produced very little amylase activity, and hence was unable to degrade granular starch. the strain b8.a extracellular enzyme fraction degraded wheat, tapioca and potato starch ...201121732245
isolation and characterization of lead-tolerant ochrobactrum intermedium and its role in enhancing lead accumulation by eucalyptus this study, the potential of rhizospheric bacteria in promoting the growth and pb accumulation by the woody plant eucalyptus camaldulensis under hydroponic conditions was investigated for the first time. three pb-tolerant bacteria were isolated from the rhizosphere of e. camaldulensis grown in pb-contaminated soils in the bo ngam pb mine, thailand. based on analysis of partial 16s rrna gene sequence, the three isolates were identified as microbacterium paraoxydans bn-2, ochrobactrum intermedi ...201121764101
structure and mechanism of iron translocation by a dps protein from microbacterium arborescens.dps enzymes are a major class of dodecameric proteins which bacteria use to detoxify their cytosol through the uptake of reactive iron species. in the stationary growth phase of bacteria, dps enzymes (dna protection during starvation) are primarily used to protect dna by bio-crystallization. to characterize the wildtype dps protein from microbacterium arborescens, which displays additional catalytic functions (amide hydrolysis and synthesis), we determined the crystal structure to a resolution o ...201121768097
phylogenetic perspectives of nitrogen-fixing was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ...201121779790
induction and resuscitation of the viable but nonculturable state in a cyanobacteria-lysing bacterium isolated from cyanobacterial bloom.the viable but nonculturable (vbnc) state has been found to be a growth strategy used by many aquatic pathogens; however, few studies have focused on vbnc state on other aquatic bacterial groups. the purpose of this study was to explore the vbnc state of cyanobacteria-lysing bacteria and the conditions that regulate their vbnc state transformation. three cyanobacteria-lysing heterotrophic bacterial strains (f1, f2 and f3) were isolated with liquid infection method from a lake that has experience ...201121850447
hessian fly-associated bacteria: transmission, essentiality, and composition.plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ...201121858016
[Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut].We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping.201121866710
biofouling characteristics and identification of preponderant bacteria at different nutrient levels in batch tests of a recirculating cooling water system.understanding the influence of nutrient levels on biofouling control is an important requirement for management strategies in a recirculating cooling water system. nutrient limitation may be one way to control biofouling development without increasing biocide dosing. therefore, this study was carried out to investigate the effects of nutrient levels on biofouling characteristics and to identify the preponderant bacteria in the batch tests with a simulated cooling water system. the biofouling cha ...201121879565
characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of herculaneum in italy.microbiological characterization of frescos in four different locations (collegio degli augustali, casa del colonnato tuscanico, casa dello scheletro and casa del gran portale) of excavation sites of herculaneum was carried out. the use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. the microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming an ...201121882662
characterization of a recombinant (-)γ-lactamase from microbacterium hydrocarbonoxydans.a (-)γ-lactamase, mhg, from microbacterium hydrocarbonoxydans was over-expressed in e. coli and was characterized after purification. the maximum activity was at ph 8.0 and 60°c and the half life of mhg was ~30 min at 75°c. the enzyme was activated by dtt. the catalytic triad of the (-)γ-lactamase is comprised of residues ser98, asp230, and his259 and an oxyanion hole was formed by tyr32 and met99 according to the alignment results. under native conditions, the (-)γ-lactamase consists of two 31  ...201121968479
the actinobacterium microbacterium sp. 16sh accepts pbbr1-based pprobe vectors, forms biofilms, invades roots, and fixes n(2) associated with micropropagated sugarcane plants.members of the genus microbacterium lineage of gram-positive actinobacteria are increasingly being reported to display significant traits associated with environmental biotechnology and bioengineering. 16sh is a nitrogen-fixing bacterial strain isolated from a surface-sterilized stem of sugarcane grown in guangxi, china. analysis of 16s rrna gene sequences revealed that 16sh belonged to the genus microbacterium. pprobe-ptet(r) plasmids were constructed by cloning the promoter region of the tet ...201122002067
butyric acid- and dimethyl disulfide-assimilating microorganisms in a biofilter treating air emissions from a livestock facility.biofiltration has proven an efficient tool for the elimination of volatile organic compounds (vocs) and ammonia from livestock facilities, thereby reducing nuisance odors and ammonia emissions to the local environment. the active microbial communities comprising these filter biofilms have not been well characterized. in this study, a trickle biofilter treating air from a pig facility was investigated and proved efficient in removing carboxylic acids (>70% reduction), mainly attributed to the pri ...201122003018
Diagnostic value of an immunochromatographic test over clinical predictors for tuberculosis in HIV patients.The value of an immunochromatographic test for tuberculosis (ICT-TB) combined with clinical predictors has yet to be evaluated in Thailand. This study aimed to assess any additional diagnostic value of an ICT-TB test over that of clinical predictors in a group of human immunodeficiency virus (HIV) patients as well as in subgroups of HIV patients classified by clinical risk scores.201122003307
High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.Stable-isotope probing (SIP) has proved a valuable cultivation-independent tool for linking specific microbial populations to selected functions in various natural and engineered systems. However, application of SIP to microbial populations with relatively minor buoyant density increases, such as populations that utilize compounds as a nitrogen source, results in reduced resolution of labeled populations. We therefore developed a tandem quantitative PCR (qPCR)-TRFLP (terminal restriction fragmen ...201222038597
Microbacterium amylolyticum sp. nov., N5T a bacterium isolated from industrial waste soil.A Gram-positive, heterotrophic, aerobic, non-motile, non-endospore forming, rod-shaped and yellow colored bacterium N5T was isolated from a soil sample collected from the industrial waste site in Noida at the outskirts of Delhi, India. Strain N5T showed highest 16S rRNA gene similarity to Microbacterium indicum LMG 23459T (97.59 %) and Microbacterium gubbeenense LMG 19263T (97.18 %). The 16S rRNA gene sequence similarity between strain N5T and the validly published name of the species of genus M ...201122039005
the potential of epiphytic hydrocarbon-utilizing bacteria on legume leaves for attenuation of atmospheric hydrocarbon pollutants.the leaves of two legumes, peas and beans, harbored on their surfaces up to 9×10⁷ cells g⁻¹ of oil-utilizing bacteria. less numbers, up to 5×10⁵ cells g⁻¹ inhabited leaves of two nonlegume crops, namely tomato and sunflower. older leaves accommodated more of such bacteria than younger ones. plants raised in oily environments were colonized by much more oil-utilizing bacteria than those raised in pristine (oil-free) environments. similar numbers were counted on the same media in which nitrogen sa ...201222054577
microbacterium immunditiarum sp. nov., a novel actinobacterium isolated from a landfill surface soil.a gram-positive, non-endospore forming bacterium designated as strain sk 18t was isolated from surface soil of a landfill by dilution plating on tryptic soy broth agar (tsba). preliminary characterization of strain sk 18t using biochemical tests, fame analysis and partial 16s rrna gene sequencing placed it within the genus microbacterium. analysis of the cell wall indicated that the peptidoglycan was of the b-type of cross-linkage containing amino acids lysine and ornithine with muramic acid in ...201122058326
Microbacterium suwonense sp. nov., isolated from cow dung.An actinomycete strain, designated M1T8B9(T), was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17(T) (98.2%) and Microbacterium esteraromaticum DSM 8609(T) (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosp ...201122068506
identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ...201222074685
culturable bacterial communities on leaf sheaths and panicles of rice plants in japan.culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ...201122081407
L-Tryptophan Production by Auxotrophic and Analogue Resistant Mutants of Aureobacterium flavescens.A number of tyrosine plus phenylalanine double auxotrophic mutants were isolated by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) treatment of a locally isolated strain of Aureobacterium flavescens of which 11A(39) and 11A(17) were selected on the basis of their tryptophan production in a mineral salt medium over other isolated mutant strains. The mutational block in the aromatic amino acid biosynthetic pathway of the selected double auxotrophs were determined. By controlling pH of the production m ...201122084602
High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs.High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase-amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. ...201122112948
Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea.Twenty-five crude-oil-degrading bacteria were isolated from oil-contaminated sites in the Persian Gulf and the Caspian Sea. Based on a high growth rate on crude oil and on hydrocarbon degradation ability, 11 strains were selected from the 25 isolated strains for further study. Determination of the nucleotide sequence of the 16S rRNA gene showed that these isolated strains belonged to genera Acinetobacter, Pseudomonas, Gordonia, Rhodococcus, Cobetia, Halomonas, Alcanivorax, Marinobacter and Micro ...201222130193
adsorption behavior of microbes on a qcm chip modified with an artificial siderophore-fe(3+) complex.three hydroxamate-type artificial siderophores with terminal nh(2) groups, tris[2-{3-(n-acyl-n-hydroxamino)propylamido}propyl]aminomethane (1-3, acyl-r group = me, et, and ph, respectively), and their fe(3+) complexes, 4-6, were prepared. the stability constant (log β) of 4 was estimated to be about 31 by its edta titration. the biological activities of 4-6 for microbacterium flavescens, which is a hydroxamate-type siderophore, auxotrophic gram-positive microbe, clearly indicated that they perme ...201122182317
Microbacterium murale sp. nov., isolated from an indoor wall.A Gram-positive rod shaped bacterium (01-GI-001T) isolated from a wall colonized with moulds was studied for its taxonomic position. The 16S rRNA gene sequence analysis showed, that the isolate clearly grouped into the Microbacterium cluster. On the basis of pairwise comparisons of the 16S rRNA gene sequences, isolate 01-Gi-001T was most closely related to the type strains of Microbacterium hydrocarbonoxydans (98.8%), Microbacterium profundi (98.7%), Microbacterium phyllosphaerae (98.3%) and Mic ...201122199224
enzymatic biotransformation of ginsenoside rb1 to 20(s)-rg3 by recombinant β-glucosidase from microbacterium esteraromaticum.microbacterium esteraromaticum was isolated from ginseng field. the β-glucosidase gene (bgp1) from m. esteraromaticum was cloned and expressed in escherichia coli bl21 (de3). the bgp1 gene consists of 2,496 bp encoding 831 amino acids which have homology to the glycosyl hydrolase family 3 protein domain. the recombinant β-glucosidase enzyme (bgp1) was purified and characterized. the molecular mass of purified bgp1 was 87.5 kda, as determined by sds-page. using 0.1 mg ml(-1) enzyme in 20 mm sodiu ...201222249721
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