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adsorption of microorganisms onto an artificial siderophore-modified au substrate.the hydroxamate-type artificial siderophore, tris[2-{3-(n-acetyl-n-hydroxamino)propylamido}propyl]aminomethane (tappa) and its fe(iii) complex, fe(iii)-tappa were prepared and characterized by several spectroscopic methods. fe(iii)-tappa exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, microbacterium flavescens, suggesting that fe(iii)-tappa can permeate the cell membrane of the microorganism. the adsorption of the fe(iii)-siderophore complex onto a de ...200717890077
characterisation of aerobically grown non-spore-forming bacteria from paper mill pulps containing recycled fibres.a total of 179 non-spore-forming bacteria aerobically growing on nutrient agar, plate count agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. after phenotypical screening the isolates were characterised by automated ribotyping and partial sequencing of the 16s rrna gene. they could be divided into seven taxonomical classes representing 63 taxa (species): actinobacteria (11 species), ba ...200918820960
culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits.during cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. the diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. about 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in ...200717890340
[diversity of actinomycetes in wuling mountain].to isolate new actinomycetes for discovering compounds of pharmaceutical importance.200818837376
diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates.bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ...200717905875
normal intestinal flora of wild nile crocodiles (crocodylus niloticus) in the okavango delta, botswana.bacterial and fungal cultures were performed from cloacal swabs collected from 29 wild nile crocodiles, captured in the okavango delta, botswana. sixteen species of bacteria and 6 fungal species were cultured. individual crocodiles yielded 1-4 bacterial species, and 0-2 fungal species. the most commonly isolated bacteria were microbacterium, enterococcus faecalis, aeromonas hydrophila, and escherichia coli. no salmonellae were cultured. the most commonly occurring fungus was cladosporium. severa ...200818846850
effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation.spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ...200818083857
a novel recombinant system for functional expression of myonecrotic snake phospholipase a(2) in escherichia coli using a new fusion affinity tag.a novel recombinant expression system in escherichia coli was developed using conger eel galectin, namely, congerin ii, as an affinity tag. this system was applied for the functional expression of myotoxic lysine-49-phospholipase a(2) ([lys(49)]pla(2)), termed bpii and obtained from protobothrops flavoviridis (pf) venom. recombinant pf bpii fused with a congerin tag has been successfully expressed as a soluble fraction and showed better quantitative yield when folded correctly. the solubility of ...200818207418
virulence of plant pathogenic bacteria attenuated by degradation of fatty acid cell-to-cell signaling factors.diffusible signal factor (dsf) is a fatty acid signal molecule involved in regulation of virulence in several xanthomonas species as well as xylella fastidiosa. in this study, we identified a variety of bacteria that could disrupt dsf-mediated induction of virulence factors in xanthomonas campestris pv. campestris. while many bacteria had the ability to degrade dsf, several bacterial strains belonging to genera bacillus, paenibacillus, microbacterium, staphylococcus, and pseudomonas were identif ...200818257682
microbacterium hatanonis sp. nov., isolated as a contaminant of hairspray.an aerobic, rod-shaped, gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain fcc-01(t), originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. a 16s rrna gene sequence analysis revealed that the isolate belonged to the genus microbacterium and represented an evolutionary lineage that was distinct from recognized microbacterium species. cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars ...200818319473
identification of cis-regulatory elements from the c. elegans t-box gene mab-9 reveals a novel role for mab-9 in hypodermal function.we have identified conserved non-coding elements (cnes) in the regulatory region of caenorhabditis elegans and caenorhabditis briggsae mab-9, a t-box gene known to be important for cell fate specification in the developing c. elegans hindgut. two adjacent cnes (a region 78 bp in length) are both necessary and sufficient to drive reporter gene expression in posterior hypodermal cells. the failure of a genomic mab-9::gfp construct lacking this region to express in posterior hypodermis correlates w ...200818402933
microbacterium aquimaris sp. nov., isolated from seawater.strains js54-2(t) and js63-1 were isolated from seawater at jeju, korea. cells of these strains were gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. the strains had chemotaxonomic markers that were consistent with their classification in the genus microbacterium, i.e. mk-11, mk-12 and mk-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, pept ...200818599704
microbacterium luticocti sp. nov., isolated from sewage sludge compost.strain sc-087b(t), isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. the isolate was a gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27-45 degrees c, ph 5.5-9.7 and in up to 10 % nacl. the peptidoglycan was of the b2beta type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. the muramic acid residues of the peptidoglycan were partially glycolylated. the ...200818599719
treatment of bromoamine acid wastewater using combined process of micro-electrolysis and biological aerobic filter.the wastewater originated from the production of bromoamine acid was treated in a sequential system of micro-electrolysis (me) and biological aerobic filter (baf). decolorization and cod(cr) removal rate of the proposed system was investigated with full consideration of the influence of two major controlling factors such as organic loading rate (olr) and hydraulic retention time (hrt). the removal rate of cod(cr) was 81.2% and that of chrominance could be up to 96.6% at an olr of 0.56 kg m(-3)d( ...200918621476
identities of arthrobacter spp. and arthrobacter-like bacteria encountered in human clinical specimens.after the initial description of arthrobacter spp. isolated from clinical specimens in the mid-1990s, very few further reports on arthrobacter spp. have appeared in the clinical microbiology literature. the aim of the present study was to elucidate the distribution of arthrobacter spp. and arthrobacter-like bacteria encountered in clinical specimens by studying 50 consecutively isolated or received strains of large-colony-forming, whiteish-grayish, non-cheese-like-smelling, nonfermentative gram- ...200818650355
chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading.a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. complete cr(vi) reduction in aerobic cultures was achieved at a high concentration of 200 mg/l after incubation for only 65 hours. under anaerobic conditions up to 150 mg, cr(vi)/l was completely removed after incubating for 130 to 155 hours, still higher t ...200818701792
coevolution between attine ants and actinomycete bacteria: a reevaluation.we reassess the coevolution between actinomycete bacteria and fungus-gardening (attine) ants. actinomycete bacteria are of special interest because they are metabolic mutualists of diverse organisms (e.g., in nitrogen-fixation or antibiotic production) and because pseudonocardia actinomycetes are thought to serve disease-suppressing functions in attine gardens. phylogenetic information from culture-dependent and culture-independent microbial surveys reveals (1) close affinities between free-livi ...200818752608
identities of microbacterium spp. encountered in human clinical specimens.in the present study, 50 strains of yellow-pigmented gram-positive rods that had been isolated from human clinical specimens and collected over a 5-year period were further characterized by phenotypic and molecular genetic methods. all 50 strains belonged to the genus microbacterium, and together they represented 18 different species. microbacterium oxydans (n = 11), m. paraoxydans (n = 9), and m. foliorum (n = 7) represented more than half of the strains included in the present study. the isola ...200818799696
enhanced removal of dimethyl sulfide from a synthetic waste gas stream using a bioreactor inoculated with microbacterium sp. ntut26 and pseudomonas putida.the removal of dimethyl sulfide (dms) from industrial gas streams has received a high priority due to its very low odorous threshold value and relatively low biodegradability compared to other reduced sulfur compounds. a variety of bacteria that utilize dms as a carbon/energy source have been studied and the degradation pathway elucidated. however, to date, there have been few reports on the industrial application of such bacteria inoculated into a bioreactor for dms treatment. an additional pro ...200918815822
structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter.the nucleobase-cation-symport-1 (ncs1) transporters are essential components of salvage pathways for nucleobases and related metabolites. here, we report the 2.85-angstrom resolution structure of the ncs1 benzyl-hydantoin transporter, mhp1, from microbacterium liquefaciens. mhp1 contains 12 transmembrane helices, 10 of which are arranged in two inverted repeats of five helices. the structures of the outward-facing open and substrate-bound occluded conformations were solved, showing how the outwa ...200818927357
microbacterium kribbense sp. nov., isolated from soil.a gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated msl-04(t), was isolated from a soil sample collected from an agricultural field on bigeum island (republic of korea) and was subjected to polyphasic characterization to unravel its taxonomic position. the optimum growth temperature and ph were 28-30 degrees c and ph 7.2-7.4. chemotaxonomic characteristics such as b2beta-type peptidoglycan with glycolyl residues, mk-11 and mk-12 as the major menaquinones and a fatty ac ...200818984689
chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor.using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ...200818392868
biological interactions to select biocontrol agents against toxigenic strains of aspergillus flavus and fusarium verticillioides from maize.biological control represent an alternative to the use of pesticides in crop protection. a key to progress in biological control to protect maize against fusarium verticillioides and aspergillus flavus maize pathogens are, to select in vitro, the best agent to be applied in the field. the aim of this study was to examine the antagonistic activity of bacterial and yeast isolates against f.verticillioides and a. flavus toxigenic strains. the first study showed the impact of bacillus amyloliquefaci ...200919247799
medium optimization for the production of cyclic adenosine 3',5'-monophosphate by microbacterium sp. no. 205 using response surface methodology.response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3',5'-monophosphate (camp) with microbacterium sp. no. 205. a fractional factorial design (2(11-7)) was applied to evaluate the effects of different components in the medium. k(2)hpo(4), mgso(4) and naf were found to significantly influence on the camp production. the steepest ascent method was used to access the optimal region of the medium composition. the concentrations of the three ...200918778935
impact of two bacterial biocontrol agents on bacterial and fungal culturable groups associated with the roots of field-grown maize.to assess the impact of bacillus amyloliquefaciens and microbacterium oleovorans on bacterial and fungal groups associated to the roots of field-grown maize.200919292823
conversion of puerarin into its 7-o-glycoside derivatives by microbacterium oxydans (cgmcc 1788) to improve its water solubility and pharmacokinetic properties.microbacterium oxydans strain nj 6 isolated from soil samples converted puerarin into two novel compounds, puerarin-7-o-glucoside and puerarin-7-o-isomaltoside, via an unreported o-glycosylation of the phenolic hydroxyl group at the 7-position of puerarin. sucrose, maltotriose, and maltose could be used as glucosyl donors for glycosylation of puerarin, but uridine-diphosphate glucose, glucose, fructose, lactose, cyclodextrin, and starch could not. regardless of the position of b-ring in the (iso ...200818795283
isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the southwest indian ocean ridge.microorganisms play an important role in the geobiocycling of arsenic element. however, little is known about the bacteria involved in this process in oceanic environments. in this report, arsenite-resistant bacteria were detected in deep-sea sediments on the southwest indian ridge. from arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2-80 mm. phylogenetic analysis based on 16s rrna showed that they mainly belonged to proteobacteria and actinob ...200918841325
microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ...200818218944
viable ultramicrocells in drinking water.to examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems.200919040704
application of compochip microarray to investigate the bacterial communities of different composts.a microarray spotted with 369 different 16s rrna gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. the microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). labeled target dna was prepared by polymerase chain reaction amplification of 16s rrna genes using a cy5-labeled universa ...200918818861
characterization of the microbial community involved in the suppression of pythium aphanidermatum in cucumber grown on rockwool.abstract the root pathogen pythium aphanidermatum induced lower levels of disease in cucumber (cucumis sativus) plants on unsterilized, re-used rockwool slabs than on heat-sterilized, re-used rockwool. several recolonization treatments of the sterilized rockwool enhanced the suppressiveness of the rockwool. microbial community structures in the different rockwool treatments were investigated by plate counts on selective media. disease suppressiveness in the different rockwool treatments showed t ...200518943014
occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland.the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ...200919572487
interactions between strains of 2,4-diacetylphloroglucinol-producing pseudomonas fluorescens in the rhizosphere of wheat.abstract strains of fluorescent pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-dapg) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. the genotypic diversity that exists among 2,4-dapg producers can be exploited to improve rhizosphere competence and biocontrol activity. knowing that d-genotype 2,4-dapg-producing strains are enriched in some take-all decline soils and that p. fluorescens q8r1-96, a representative d-genotyp ...200318943865
isolation and characterization of biosurfactant-producing alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis.biosurfactant-producing bacteria belonging to the genera alcanivorax, cobetia and halomonas were isolated from marine sediments with a history of hydrocarbon exposure (aristizábal and gravina peninsulas, argentina). two alcanivorax isolates were found to form naturally occurring consortia with strains closely related to pseudomonas putida and microbacterium esteraromaticum. alkane hydroxylase gene analysis in these two alcanivorax strains resulted in the identification of two novel alkb genes, s ...200918983915
elucidation of 2-hydroxybiphenyl effect on dibenzothiophene desulfurization by microbacterium sp. strain zd-m2.the effect of 2-hydroxybiphenyl (2-hbp), the end product of dibenzothiophene (dbt) desulfurization via 4s pathway, on cell growth and desulfurization activity was investigated by microbacterium sp. the experimental results indicate that 2-hbp would inhibit the desulfurization activity. providing 2-hbp was added in the reaction media, the dbt degradation rate decreased along with the increase of 2-hbp addition. by contrast, cell growth would be promoted in the addition of 2-hbp at a low concentra ...200818296046
the c. elegans hox gene egl-5 is required for correct development of the hermaphrodite hindgut and for the response to rectal infection by microbacterium nematophilum.members of the hox gene family encode transcription factors that specify positional identity along the anterior-posterior axis of nearly all metazoans. one among the caenorhabditis elegans hox genes is egl-5. a deletion allele of egl-5 was isolated in a screen for animals which fail to develop swollen tails when exposed to the bacterial pathogen microbacterium nematophilum. we show that compromised rectal development, which occurs as a result of loss of egl-5 function, results in a failure of re ...200919232338
lateral transfer of genes for hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) degradation.recent studies demonstrated that degradation of the military explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by species of rhodococcus, gordonia, and williamsia is mediated by a novel cytochrome p450 with a fused flavodoxin reductase domain (xpla) in conjunction with a flavodoxin reductase (xplb). pulse field gel analysis was used to localize xpla to extrachromosomal elements in a rhodococcus sp. and distantly related microbacterium sp. strain ma1. comparison of rhodococcus rhodochrous 1 ...200919270122
plasmid transformation and expression of the firefly luciferase in microbacterium testaceum type and endophytic colonizing field strains.microbacterium testaceum is a predominant endophytic bacterial species isolated from corn and sorghum in the midwestern united states. the development of genetic transfer systems for m. testaceum may enable its use for biocontrol and other applications. the type strain (ifo 12675) and field isolates (se017, se034, and ce648) were grown to mid-exponential phase, concentrated (1.0 x 1011 cfu x ml(-1)), electroporated (escherichia coli-clavibacter shuttle plasmid pdm302), and plated on tsa with 10 ...200818997853
the acyltransferase gene bus-1 exhibits conserved and specific expression in nematode rectal cells and reveals pathogen-induced cell swelling.susceptibility to the rectal pathogen microbacterium nematophilum provides a means of examining hindgut differentiation in c. elegans. mutants of bus-1 are resistant to infection with this pathogen. we show here that bus-1 encodes a predicted acyltransferase expressed in rectal epithelial cells (k, f, and u), suggesting its involvement in regional surface modification. bus-1 reporter genes were used to show spatial regulation by hindgut developmental control genes: egl-38, mab-9, and mab-23. a b ...200819035336
probing the critical residues for intramolecular fructosyl transfer reaction of a levan fructotransferase.levan fructotransferase (lftase) preferentially catalyzes the transfructosylation reaction in addition to levan hydrolysis, whereas other levan-degrading enzymes hydrolyze levan into a levan-oligosaccharide and fructose. based on sequence comparisons and enzymatic properties, the fructosyl transfer activity of lftase is proposed to have evolved from levanase. in order to probe the residues that are critical to the intramolecular fructosyl transfer reaction of the microbacterium sp. al-210 lftase ...200818600048
endoxanthanase, a novel beta-d-glucanase hydrolyzing backbone linkage of intact xanthan from newly isolated microbacterium sp. xt11.a novel endoxanthanase was produced and isolated from the culture of microbacterium sp. xt11 growing on xanthan. optimum ph and temperature for the enzyme activity were 6.0 and 35-40 degrees c, respectively. the endoxanthanase cleaves the backbone endo-beta-1,4-glucosidic linkage of the xanthan molecule, which is specific to the intact xanthan. however, the lyase-treated xanthan or carboxymethyl cellulose could not be cleaved by endoxanthanase.200919050833
detection and hazard assessment of pathogenic microorganisms in medical wastes.this study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. medical wastes collected from 5 major hospitals in south korea were classified and stored at three different temperatures (-20, 6, and 30 degrees c). presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and b ...200919827491
[phylogenetic diversity of the culturable rare actinomycetes in marine sponge hymeniacidon perlevis by improved isolation media].based on the molecular diversity information, seven actinomycete-selective culture media and isolation conditions were modified to isolate and cultivate diverse rare actinomycetes from hymeniacidon perlevis.200919873748
[genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing].plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.200919873749
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
crystallization of the hydantoin transporter mhp1 from microbacterium liquefaciens.the integral membrane protein mhp1 from microbacterium liquefaciens transports hydantoins and belongs to the nucleobase:cation symporter 1 family. mhp1 was successfully purified and crystallized. initial crystals were obtained using the hanging-drop vapour-diffusion method but diffracted poorly. optimization of the crystallization conditions resulted in the generation of orthorhombic crystals (space group p2(1)2(1)2(1), unit-cell parameters a = 79.7, b = 101.1, c = 113.8 a). a complete data set ...200819052379
characterization of the ars gene cluster from extremely arsenic-resistant microbacterium sp. strain a33.the arsenic resistance gene cluster of microbacterium sp. a33 contains a novel pair of genes (arstx) encoding a thioredoxin system that are cotranscribed with an unusual arsrc2 fusion gene, acr3, and arsc1 in an operon divergent from arsc3. the whole ars gene cluster is required to complement an escherichia coli ars mutant. arsrc2 negatively regulates the expression of the pentacistronic operon. arsc1 and arsc3 are related to thioredoxin-dependent arsenate reductases; however, arsc3 lacks the tw ...201019966021
agrococcus versicolor sp. nov., an actinobacterium associated with the phyllosphere of potato plants.the taxonomic position of a group of actinobacterial strains isolated from the phyllosphere of potato plants was investigated by using a polyphasic approach. although the similarity values for their 16s rrna gene sequences suggested an intermediate position between microbacterium and agrococcus, the phylogenetic tree demonstrated a clear clustering of the representative strain, k 114/01(t), within the genus agrococcus. the presence of 2,4-diaminobutyric acid as the diagnostic diamino acid in the ...200819060068
molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques.polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ...201020018377
microbacterium profundi sp. nov., isolated from deep-sea sediment of polymetallic nodule environments.a gram-positive, aerobic, neutrophilic and rod-shaped bacterium, strain shh49(t), was isolated from a deep-sea sediment sample collected from the east pacific polymetallic nodule region. the strain was able to grow within a temperature range of 4-35 degrees c and tolerated up to 7.5 % (w/v) nacl. strain shh49(t) was characterized chemotaxonomically by having mk-12 and mk-13 as predominant isoprenoid quinones, anteiso-c(15 : 0), iso-c(15 : 0), iso-c(16 : 0) and anteiso-c(17 : 0) as major fatty ac ...200819060085
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
sand aggregation by exopolysaccharide-producing microbacterium arborescens--agsb.in the rhizosphere, exopolymers are also known to be useful to improve the moisture-holding capacity. the ability of the isolates from coastal sand dunes to produce exopolymers was determined. among which the isolate, showing very high production of exopolysaccharide (eps), microbacterium arborescens--agsb, a facultative alkalophile was further studied for exopolymer production. the isolate a gram-positive non-spore forming, slender rod, catalase positive, oxidase negative, showed growth in 12% ...200919340484
microbacterium binotii sp. nov., isolated from human blood.two strains of non-spore-forming, rod-shaped, gram-positive bacteria, cip 101303(t) and cip 102116, were isolated from human blood in 1976 and 1977, respectively. these strains had chemotaxonomic markers that were consistent with classification in the genus microbacterium, i.e. mk-10, mk-11 and mk-12 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, galactose, mannose and rhamnose as the cell-wall sugars and ornithine as the diamino acid in the cell-wall pept ...200919406785
polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent.biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ...201020093025
growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp. during ripening in a cheese model medium.the growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp., some common but rarely mentioned cheese bacteria, were investigated in a cheese model deacidified by debaryomyces hansenii during the ripening process. our results show that these bacteria had distinct growth and cheese flavour production patterns during the ripening process. p. vulgaris had the greatest capacity to produce not only the widest variety but also the highest quantities of volatile ...200919083231
[application of biological aerated filter in treating biomass wastewater and its microbial population characteristics].biomass wastewater contains plenty of cellulose, hemicellulose, starch, saccharide, organic acid and protein. it has high cod value and leads to environment pollution and resource waste. biomass wastewater was treated in this investigate using a biological aerated filter (baf) on laboratory scale, and the effect of treatment in different hydraulic retention time (hrt) had been studied. the results show that the optimum hrt is 10 h. the value of cod and tn in effluent is 74.90 mg/l and 1.21 mg/l ...200819186816
microbacterium invictum sp. nov., isolated from homemade compost.strain dc-200t was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. the isolate was a gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10-37 degrees c, ph 6.0-9.5 and with up to 5% of nacl. the peptidoglycan was of the type b1 alpha and the muramic acid residues were glycolylated. the major fatty acids were anteiso-c15:0 and anteiso-c17:0. the predominant respiratory me ...200919567581
gene cloning and characterization of dihydrolipoamide dehydrogenase from microbacterium luteolum: a useful enzymatic regeneration system of nad+ from nadh.dihydrolipoamide dehydrogenase (lpd), a useful biocatalyst for regenerating nad(+), was purified from microbacterium luteolum jcm 9174, and the gene encoding lpd was cloned from the genomic dna. the gene contained an opening reading frame consisting of 1395 nucleotides encoding 465 amino acid residues with a predicted molecular weight of 49912.1 da, which displayed 36-78% homology to known lpds. moreover, the fad- and nad(+)-binding sites and the two catalytic residues in the lpds were conserved ...201020159566
chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxb) in rhizobacteria isolated from crop plants.twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16s rrna analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the beta and gamma subclasses of proteobacteria and actinobacteria. among these, five genera, including dyella, burkholderia, alcaligenes, microbacterium and leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. the thiosulfate-oxidizing dyella, burkholderia, alcaligenes, microbacterium, lei ...200818832027
stereospecific degradation of phenylsuccinate by actinomycetes.racemic phenylsuccinate was stereospecifically degraded by the actinomycetes ps9 and ps17 isolated from soil obtained from okinawa island, japan. strain ps9, identified as a citricoccus sp., preferentially degraded the r-form, while strain ps17, identified as a microbacterium sp., preferentially degraded the s-form of phenylsuccinate. analysis of the culture broths of these species with phenylsuccinate as the sole carbon source revealed that benzoic acid was produced as a metabolic intermediate. ...200919580991
mutation of asparagine 76 in the center of glutamine transporter snat3 modulates substrate-induced conductances and na+ binding.the glutamine transporter slc38a3 (snat3) plays an important role in the release of glutamine from brain astrocytes and the uptake of glutamine into hepatocytes. it is related to the vesicular gaba (gamma-aminobutyric acid) transporter and the slc36 family of proton-amino acid cotransporters. the transporter carries out electroneutral na+-glutamine cotransport-h+ antiport. in addition, substrate-induced uncoupled cation currents are observed. mutation of asparagine 76 to glutamine or histidine i ...200919596860
microbacterium soli sp. nov., an alpha-glucosidase-producing bacterium isolated from soil of a ginseng field.five gram-type-positive, aerobic, rod-shaped, non-motile strains of microbacterium (dcy 17(t), ms1, ms2, ms3 and ms4) were isolated from soil from a ginseng field in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, these strains were shown to be related to microbacterium esteraromaticum dsm 8609(t) (96.1 %), m. xylanilyticum dsm 16914(t) (96.0 %), m. aquimaris js54-2(t) (95.6 %), m. insulae ds-66(t) (95.5 %), m. ketosireducens ifo 14548(t) (95.5 %) and m. arabinogalactano ...201019654351
population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis.surface contamination of smear cheese by listeria spp. is of major concern for the industry. complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties in situ were investigated by tem ...201020222967
isolation of identical nitrilase genes from multiple bacterial strains and real-time pcr detection of the genes from soils provides evidence of horizontal gene transfer.bacterial enzymes capable of nitrile hydrolysis have significant industrial potential. microbacterium sp. aj115, rhodococcus erythropolis aj270 and aj300 were isolated from the same location in england and harbour identical nitrile hydratase/amidase gene clusters. strain aj270 has been well studied due to its nitrile hydratase and amidase activity. r. erythropolis itcbp was isolated from denmark and carries a very similar nitrile hydratase/amidase gene cluster. in this study, an identical nitril ...200919730817
arsenic-resistant bacteria associated with roots of the wild cirsium arvense (l.) plant from an arsenic polluted soil, and screening of potential plant growth-promoting characteristics.a rhizobacterial community, associated with the roots of wild thistle cirsium arvense (l.) growing in an arsenic polluted soil, was studied by fluorescence in situ hybridization (fish) analysis in conjunction with cultivation-based methods. in the bulk, rhizosphere, and rhizoplane fractions of the soil, the qualitative picture obtained by fish analysis of the main phylogenetic bacterial groups was similar and was predominantly comprised of alphaproteobacteria, betaproteobacteria, and gammaproteo ...201020303688
the c. elegans glycosyltransferase bus-8 has two distinct and essential roles in epidermal morphogenesis.ventral enclosure in caenorhabditis elegans involves migration of epidermal cells over a neuroblast substrate and subsequent adhesion at the ventral midline. organisation of the neuroblast layer by ephrins and their receptors is essential for this migration. we show that bus-8, which encodes a predicted glycosyltransferase, is essential for embryonic enclosure and acts in or with ephrin signalling to mediate neuroblast organisation and to permit epidermal migration. bus-8 acts non-cell-autonomou ...200818395708
isolation of some pathogenic bacteria from the great spruce bark beetle, dendroctonus micans and its specific predator, rhizophagus grandis.some bacteria were isolated from dendroctonus micans and its specific predator, rhizophagus grandis. six bacteria from d. micans were identified as bacillus pumilus, enterobacter intermedius, citrobacter freundii, cellulomonas flavigena, microbacterium liquefaciens and enterobacter amnigenus, three bacteria from r. grandis as klebsiella pneumoniae, pantoea agglomerans and serratia grimesii, on the basis of fatty acid methyl ester analysis and carbon utilization profile by using microbial identif ...201020336502
microbacterium insulae sp. nov., isolated from soil.a gram-positive, non-motile, rod- or coccoid-shaped microbacterium-like bacterium, designated strain ds-66(t), was isolated from soil of dokdo, korea, and its exact taxonomic position was investigated by using a polyphasic approach. strain ds-66(t) grew optimally at 30 degrees c and ph 6.5-7.0 in the presence of 0.5-1.0 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain ds-66(t) belonged to the genus microbacterium. strain ds-66(t) had a peptidoglycan type ba ...200919542140
assessment of the microbial diversity at the surface of livarot cheese using culture-dependent and independent approaches.the microbial diversity of the surface of a commercial red-smear cheese, livarot cheese, sold on the retail market was studied using culture-dependent and independent approaches. forty yeasts and 40 bacteria from the cheese surface were collected, dereplicated using single-strand conformation polymorphism (sscp) analysis and identified using rrna gene sequencing for the culture-dependent approach. the culture-independent approach involved cloning and sequencing of the 16s rrna gene and sscp anal ...200919481828
hydrolysis of fenamiphos and its toxic oxidation products by microbacterium sp. in pure culture and groundwater.a bacterium with an exceptional ability to hydrolyse fenamiphos and its toxic oxidation products fenamiphos sulfoxide and fenamiphos sulfone, all possessing poc bond was isolated from soil. based on 16s rrna gene determination, this bacterium was putatively identified as microbacterium esteraromaticum. the phenols (fenamiphos phenol, sulfoxide phenol and sulfone phenol) formed during bacterial hydrolysis resisted further degradation in mineral salts medium and sterile groundwater, but were trans ...200919195880
evaluation of microbial dynamics during the ripening of a traditional taiwanese naturally fermented ham.isolation and identification of the autochthonous starter from a naturally fermented meat allows control of the fermentation process and promises microbiological safety for this specialty. thus the purpose of this study was to identify the lactic acid bacteria and coagulase-negative cocci present in a traditional taiwanese naturally fermented ham (tnfh) and to study the microbial dynamics at different ripening stages; the approach was a combination of conventional microbiological cultivation, po ...201020417394
isolation and characterization of fenamiphos degrading bacteria.the biological factors responsible for the microbial breakdown of the organophosphorus nematicide fenamiphos were investigated. microorganisms responsible for the enhanced degradation of fenamiphos were isolated from soil that had a long application history of this nematicide. bacteria proved to be the most important group of microbes responsible for the fenamiphos biodegradation process. seventeen bacterial isolates utilized the pure active ingredient fenamiphos as a carbon source. sixteen isol ...201020464454
selected microbial glycolipids: production, modification and characterization.this chapter deals with two types of biosurfactants that are not in the spotlight of general research: glycoglycerolipids and oligosaccharide lipids. the main focus is on glycolglycerolipids from marine bacteria like microbacterium spec. dsm 12583, micrococcus luteus (hel 12/2) and bacillus pumilus strain aas3 and on oligosaccharide lipids from tsukamurella spec. dsm 44370 and nocardia corynebacteroides sm1. general and special structures, microbial producers, production conditions and chemo-enz ...201020545283
characterization of microbacterium sp. f10a and its role in polycyclic aromatic hydrocarbon removal in low-temperature soil.a polycyclic aromatic hydrocarbon degrading bacterium isolated from oil-polluted soil was identified as microbacterium sp. f10a based on 16s rdna gene sequence analysis. plant growth promoting characteristics of the strain, degradation rate of phenanthrene and pyrene, and cell surface hydrophobicity characteristics of the strain were further characterized. the strain was also evaluated for promoting the growth of wheat and phenanthrene and pyrene removal from soil artificially contaminated with ...200919483781
identities of epilithic hydrocarbon-utilizing diazotrophic bacteria from the arabian gulf coasts, and their potential for oil bioremediation without nitrogen supplementation.gravel particles from four sites along the arabian gulf coast in autumn, winter, and spring were naturally colonized with microbial consortia containing between 7 and 400 × 10(2) cm(-2) of cultivable oil-utilizing bacteria. the 16s rrna gene sequences of 70 representatives of oil-utilizing bacteria revealed that they were predominantly affiliated with the gammaproteobacteria and the actinobacteria. the gammaproteobacteria comprised among others, the genera pseudomonas, pseudoalteromonas, shewane ...201020567813
[analysis of microbial diversity of nitrifying bacteria by terminal restriction fragment length polymorphism].we analyzed the microbial diversity and quantity of nitrifying bacteria in the enrichment reactor by terminal restriction fragment length polymorphism (t_rflp), a cultured-independent molecular technique. the result indicated that nitrobacteria enriched the best, and the diversity index decreased 62.80% compared with the initial data. nitrobacteria were predominant in the reactor. meanwhile, we studied the microbial diversity before and after adding nitrobacteria into shrimp ponds, and analyzed ...201020575436
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
size-resolved culturable airborne bacteria sampled in rice field, sanitary landfill, and waste incineration sites.size-resolved bacterial concentrations in atmospheric aerosols sampled by using a six stage viable impactor at rice field, sanitary landfill, and waste incinerator sites were determined. culture-based and polymerase chain reaction (pcr) methods were used to identify the airborne bacteria. the culturable bacteria concentration in total suspended particles (tsp) was found to be the highest (848 colony forming unit (cfu)/m(3)) at the sanitary landfill sampling site, while the rice field sampling si ...201020623053
impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against fusarium verticillioides.bacillus amyloliquefaciens and microbacterium oleovorans reduced the fusarium verticillioides count and significantly decreased fumonisin b(1) and b(2) levels in maize grains. the aim of this study was to determine the effect of water stress tolerance and heat shock survival upon cells of the biocontrol agents b. amyloliquefaciens and m. oleovorans. the a(w) of solid and liquid media and tryptic soy medium was modified to 0.99, 0.98, 0.97 and 0.96 by addition of ionic solute nacl and non-ionic s ...201020624459
microbacterium agarici sp. nov., microbacterium humi sp. nov. and microbacterium pseudoresistens sp. nov., isolated from the base of the mushroom agaricus blazei.three gram-positive, rod-shaped bacteria (strains cc-sbck-209( t), cc-12309(t) and cc-5209(t)) were isolated from the stalk of the edible mushroom agaricus blazei grown in the laboratory. 16s rrna gene sequence analysis indicated that all three isolates clearly belonged to the genus microbacterium. strains cc-sbck-209( t) and cc-12309(t) were most related closely to the type strain of microbacterium halotolerans (95.9 and 96.1 % 16s rrna gene sequence similarity, respectively). these two novel s ...201019661494
culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot.microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ...201020657618
vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in hong kong, south china.the vertical distribution of polycyclic aromatic hydrocarbons (pahs) at different sediment depths, namely 0-2 cm, 2-4 cm, 4-6 cm, 6-10 cm, 10-15 cm and 15-20 cm, in one of the most contaminated mangrove swamps, ma wan, hong kong was investigated. it was the first time to study the intrinsic potential of deep sediment to biodegrade pahs under anaerobic conditions and the abundance of electron acceptors in sediment for anaerobic degradation. results showed that the total pahs concentrations (summa ...200919683792
microbacterium azadirachtae sp. nov., a plant-growth-promoting actinobacterium isolated from the rhizoplane of neem seedlings.microbacterium strain ai-s262(t) was isolated from the rhizoplane of neem seedlings in the botanical garden of tamilnadu agricultural university, coimbatore, india, and subjected to phenotypic, chemotaxonomic and genetic characterization. cells of this strain were gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. strain ai-s262(t) contained mk-12 and mk-13 as the main respiratory quinones, anteiso-c(15 : 0), anteiso-c(17 : 0) ...201019734284
effect of bacterial cell size on electricity generation in a single-compartmented microbial fuel cell.a single-compartmented microbial fuel cell composed of a graphite felt anode modified with neutral red (nr-anode) and a porous fe(ii)-carbon cathode (fec-cathode) were compared for electricity generation from microbacterium sp. and pseudomonas sp. under identical conditions. pseudomonas sp. was more than four times the size of microbacterium sp. based on sem images. in cyclic voltammetry, the redox reaction between microbacterium sp and electrode was three times the rate observed between pseudom ...201020013300
comparison of maldi tof with conventional identification of clinically relevant bacteria.a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory.201020924806
diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania.aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ...201020941581
microbacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil.a gram-stain-positive, aerobic, rod-shaped, yellow actinobacterium, designated mna2(t), was isolated from a hexachlorocyclohexane-contaminated soil in north india. strain mna2(t) showed 95 % 16s rrna gene sequence similarity with microbacterium halotolerans yim 70130(t). phylogenetic analysis of 16s rrna gene sequences showed that strain mna2(t) belonged to a clade represented by the genus microbacterium of the family microbacteriaceae. strain mna2(t) contained anteiso-c(15 : 0), anteiso-c(17 :  ...201020023059
chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading: brits culture (south africa).a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. cr(vi) reduction rate up to 8 times higher than the rate in previous cultures was achieved by the brits culture under aerobic conditions. near complete cr(vi) reduction was observed in batches under initial concentrations up to 200mg cr(vi)/l after incubati ...200818760438
novel n-acylhomoserine lactone-degrading bacteria isolated from the leaf surface of solanum tuberosum and their quorum-quenching properties.we isolated and identified ahl-degrading bacteria from the leaf surface of solanum tuberosum. the 16 isolates inactivated both short- and long-chain ahls. two of these isolates, identified as microbacterium testaceum, showed putative ahl-lactonase activity. these two strains interrupted quorum-sensing dependent bacterial infection by plant pathogen pectobacterium carotovorum subsp. carotovorum. m. testaceum strains, which were isolated in this study, might be useful in the biocontrol of plant di ...200919734660
characterization of the caenorhabditis elegans udp-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis.galactofuranose (gal(f)), the furanoic form of d-galactose produced by udp-galactopyranose mutases (ugms), is present in surface glycans of some prokaryotes and lower eukaryotes. absence of the gal(f) biosynthetic pathway in vertebrates and its importance in several pathogens make ugms attractive drug targets. since the existence of gal(f) in nematodes has not been established, we investigated the role of the caenorhabditis elegans ugm homolog glf-1 in worm development. glf-1 mutants display sig ...200919751718
permeabilization of microbacterium oxylans shifts the conversion of puerarin from puerarin-7-o-glucoside to puerarin-7-o-fructoside.the main product of the conversion of puerarin by unpermeabilized cells of bacterium microbacterium oxydans cgmcc 1788 was puerarin-7-o-glucoside (241 +/- 31.9 microm). permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 +/- 48.0 microm) which was identified as a novel puerarin-7-o-fructoside by electrospray ionization time-of-flight ms, (13)c nmr, (1)h nmr, and gc-ms analysis of ...201019943046
microbacterium radiodurans sp. nov., a uv radiation-resistant bacterium isolated from soil.strain gimn 1.002(t), a uv radiation-tolerant bacterium, was isolated from the upper sand layers of the gobi desert, xinjiang, china and characterized in order to determine its taxonomic position. cells were gram-reaction-positive, heterotrophic, strictly aerobic, short rods. 16s rrna gene sequence analysis revealed that strain gimn 1.002(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens dsm 20754(t) (98.8 % 16s rrna gene sequence similarity) and micro ...201020061506
microbacterium ginsengiterrae sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain dcy37(t) was isolated from a soil sample of a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-staining-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain dcy37(t) belongs to the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium aerolatum dsm 14217(t) (98.8 %), microbacterium hydrocarbonoxydans d ...201020081025
selection of candidate probionts by two different screening strategies from atlantic cod (gadus morhua l.) larvae.two primary selection criteria were used to collect a pool of nearly 500 candidate probiotic bacteria from atlantic cod (gadus morhua l.) larvae, i.e. the dominant intestinal bacterial flora and isolates with antagonistic activity against vibrio anguillarum. bacteria were isolated from cod larvae from five rearing groups with variable rearing technologies. the bacteria were brought to pure culture and characterized phenotypically. based on properties such as uniqueness, dominance and fermentativ ...201020097491
identification and characterization of bacteria isolated under selective pressure of volatile organic compounds.several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ...200920112870
symbiotic relationship between microbacterium sp. sk0812 and candida tropicalis sk090404.a bacterium growing inside yeast cytoplasm was observed by light microscope without staining. the bacterium was separately stained from yeast cell by a fluorescent dye, 4',6-diamidino-2-phenylindole (dapi). the bacterium actively moved inside yeast cytoplasm and propagated in company with the yeast growth. the bacterium was separated from the yeast cytoplasm by selective disruption of yeast cells and the yeast without the intracellular bacterium (ywob) was obtained by selective inactivation of b ...201020127466
characterization of phosphobacteria isolated from eutrophic aquatic ecosystems.phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. to develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. molecular identification and phylogenetic analysis revealed three groups among the nine isolates of inorganic phosphate-solubilizing bacteria (ipsb): ipsb1 and ipsb2 belonged to the actinobacteria and flavobacteria, respectively, and the other seven belonged to the gamma-proteobacteria. among f ...200920170019
aiim, a novel class of n-acylhomoserine lactonase from the leaf-associated bacterium microbacterium testaceum.n-acylhomoserine lactones (ahls) are used as quorum-sensing signal molecules by many gram-negative bacteria. we have reported that microbacterium testaceum stlb037, which was isolated from the leaf surface of potato, has ahl-degrading activity. in this study, we cloned the aiim gene from the genomic library of stlb037, which has ahl-degrading activity and shows high homology with the alpha/beta hydrolase fold family from actinobacteria. purified aiim as a maltose binding fusion protein showed hi ...201020173075
level of chemical and microbiological contaminations in chili bo (paste).the objective of this study was to determine the level of preservatives and microbiological loads in various brands of commercially available chili bo (paste). fifteen different brands of chili bo obtained from the local market and hypermarkets were analyzed for ph, moisture and benzoic acid content, microbiological loads (aerobic, anaerobic, aerobic spores, and fungi), and thermophilic microorganisms. results showed that both moisture content and ph vary among samples. the concentrations of ben ...201020202342
construction and analysis of a bacterial community exhibiting strong chitinolytic activity.a stable bacterial community expressing strong chitinolytic activity was constructed by mixing and cultivating chitinolytic bacteria collected from different natural sources. the dna fragment pattern, after pcr-denaturing gradient gel electrophoresis (dgge) targeting 16s rrna genes using total dna prepared from whole cells, indicated that the community was composed of four dominant bacterial species. all four species were isolated on agar medium, and one strain, say3, was deduced to be a novel s ...201020208340
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