purification and properties of a new type of protease produced by microbacterium liquefaciens.a bacterium, identified as microbacterium liquefaciens mim-cg-9535-i, was isolated from a soil sample taken from the industrial site of a gelatin manufacturer. a new type of protease, which restrictively decomposes gelatin at one or two positions, was purified from the bacterial culture. the molecular mass of the purified enzyme was 21 kda by sds-polyacrylamide gel electrophoresis. the purified enzyme specifically degraded the alpha-chain of gelatin with a molecular weight of 100 kda into two pe ...200515914910
biodesulfurization of dibenzothiophene and other organic sulfur compounds by a newly isolated microbacterium strain zd-m2.a new bacterial strain capable of selectively desulfurizing dibenzothiphene (dbt) was isolated from sludge. this organism, designated zd-m2, clustered most closely with members of the genus microbacterium, as determined by 16s rrna gene sequence analysis. metabolites produced by dbt desulfurization were identified by gc-ms, and two sulfur-free products, 2-methoxybiphenyl (2-mbp) and biphenyl, were detected in addition to 2-hydroxybiphenyl (2-hbp). this strain can also totally degrade 4,6-dimethy ...200515927746
[bacterial conversion of sulfur-and phosphorous-compounds and microbial diversity in sediments from a near-shore marine-cultural region].the h2s-producing bacteria and the phosphorous-cycling bacteria in sediments from near-shore marine-cultural region were investigated. results indicate that the bacterial h2s production in aerobic condition is the dominating process to produce h2s in the sediment of cultural pond. the total counts of h2s-producing bacteria utilizing cysteine and na2s2o3 were 1.6 x 10(6) and 4.35 x 10(3) cells g(-1) respectively. the counts of sulfate-reducing bacteria in the sediments were very little, only 2.5 ...200516004320
active membrane transport and receptor proteins from bacteria.a general strategy for the expression of bacterial membrane transport and receptor genes in escherichia coli is described. expression is amplified so that the encoded proteins comprise 5-35% of e. coli inner membrane protein. depending upon their topology, proteins are produced with rgsh6 or a strep tag at the c-terminus. these enable purification in mg quantities for crystallization and nmr studies. examples of one nutrient uptake and one multidrug extrusion protein from helicobacter pylori are ...200516042616
multiple genes affect sensitivity of caenorhabditis elegans to the bacterial pathogen microbacterium nematophilum.interactions with bacteria play a major role in immune responses, ecology, and evolution of all animals, but they have been neglected until recently in the case of c. elegans. we report a genetic investigation of the interaction of c. elegans with the nematode-specific pathogen microbacterium nematophilum, which colonizes the rectum and causes distinctive tail swelling in its host. a total of 121 mutants with altered response to infection were isolated from selections or screens for a bacteriall ...200516079230
evaluation of microbial strains for linoleic acid hydroxylation and reclassification of strain previous studies, a new microbial strain ala2 was isolated which produced many new products from linoleic acid [gardner h.w., hou c.t., weisleder d. and brown w. 2000. lipids 35: 1055-1060; hou c.t. 1998. 12,13,17-trihydroxy-9(z)-octodecenoic acid and derivatives and microbial isolate for production of the acid. us patent no. 5, 852, 196]. strain ala2 was preliminary identified as clavibacter sp. based on its physiological and fatty acid profiles. to determine if strain ala2 is the optimal st ...200516096693
molecular cloning and expression of the hyu genes from microbacterium liquefaciens aj 3912, responsible for the conversion of 5-substituted hydantoins to alpha-amino acids, in escherichia coli.a dna fragment from microbacterium liquefaciens aj 3912, containing the genes responsible for the conversion of 5-substituted-hydantoins to alpha-amino acids, was cloned in escherichia coli and sequenced. seven open reading frames (hyup, hyua, hyuh, hyuc, orf1, orf2, and orf3) were identified on the 7.5 kb fragment. the deduced amino acid sequence encoded by the hyua gene included the n-terminal amino acid sequence of the hydantoin racemase from m. liquefaciens aj 3912. the hyua, hyuh, and hyuc ...200516116274
[isoprenoid pigments in representatives of the family microbacteriaceae].by using fosmidomycin and mevinolin (inhibitors of the synthesis of isoprenoid pigments), spectrophotometry, and mass spectrometry, the presence of isoprenoid pigments is shown in 71 of the 78 strains under study. all of these strains belong to 11 genera of the family microbacteriaceae. yellow, orange, and red pigments are found to have absorption spectra typical of c40-carotenoids. eight out of the sixteen strains of the genus microbacterium are able to synthesize neurosporene, a precursor of l ...200516119846
synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the aureobacterium species (-) gamma-lactamase.the crystal structure of a (-) gamma-lactamase from an aureobacterium species showed a molecule bound covalently to the active site serine residue. this enzyme complex represented the first structure of a stably bound tetrahedral intermediate for an alpha/beta hydrolase fold enzyme. the structural elucidation of tetrahedral intermediates is important for the understanding of enzymatic mechanism, substrate recognition and enzyme inhibition. in this paper, we report the synthesis and subsequent ch ...200516132085
microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm.a novel xylan-degrading bacterium, s3-e(t), was isolated from the biofilm of a membrane bioreactor. the cells of this strain were gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. the strain had chemotaxonomic markers that were consistent with classification in the genus microbacterium, i.e. mk-12, mk-11 and mk-13 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, glucose and galactose as ...200516166712
the selection of mixed microbial inocula in environmental biotechnology: example using petroleum contaminated tropical soils.the impact of inorganic n and p additions on a tropical soil contaminated with petroleum hydrocarbons was investigated using molecular and culture techniques. microcosms were incubated for 42 days and sampled at 0, 1, 7, 28 and 42 days. changes in bacterial community structure were determined using denaturing gradient gel electrophoresis (dgge) of the rrna following reverse transcription pcr using primers specific to the v3 region of the 16s rrna gene. to identify which components of the microbi ...200616226327
glyceroglycolipids preventing tert-butylhydroperoxide-induced cell death from microbacterium sp. and corynebacterium aquaticum strains.galactosyl diacylglycerols m874b and s365b obtained from the recently isolated bacteria identified as microbacterium sp. m874 and corynebacterium aquaticum s365 were found to prevent oxidative cell death induced by tert-butylhydroperoxide. their structures were determined to be 1,2-di-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol and 1-o-(14-methylhexadecanoyl)-2-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol, respectively.200016232721
coaggregation between acinetobacter johnsonii s35 and microbacterium esteraromaticum strains isolated from sewage activated sludge.the extent and nature of intergeneric coaggregations among non-flocculating sludge bacteria were studied through examination of the coaggregation abilities of acinetobacter johnsonii s35 with two other strains of non-flocculating sludge bacteria (microbacterium esteraromaticum s38 and m. esteraromaticum s51). at first, the effect of electrolyte concentration as well as the addition of edta and proteases on coaggregation were studied. changes in electrolyte concentration had little effect on the ...200316233476
in vitro influence of bacterial mixtures on fusarium verticillioides growth and fumonisin b1 production: effect of seeds treatment on maize root colonization.enterobacter cloacae, microbacterium oleovorans, pseudomonas solanacearum and bacillus subtilis were investigated in order to evaluate: (i) the inoculum size of two bacterial mixtures on fusarium verticillioides growth and fumonisin b1 production in vitro at different water activities and (ii) the efficacy of a seed treatment with the best bacterial mixture on f. verticillioides root colonization in greenhouse studies.200516238641
biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membrane.high-quality water purification systems using reverse osmosis (ro) membrane separation have faced a major challenge related to biofilm formation on the membrane surface, or biofouling. to understand this issue, the biofilm formation characteristics of four bacterial isolates previously retrieved from an ro membrane treating potable water were investigated. biofilm formation of all four isolates occurred to different extents in microtiter plates and could be related to one or more cell properties ...200516245826
surface microflora of four smear-ripened cheeses.the microbial composition of smear-ripened cheeses is not very clear. a total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (pfge), repetitive sequence-based pcr, and 16s rrna gene sequencing for identifying and typing the bacteria and fourier transform infrared spectroscopy and mitochondrial dna restriction fragment length polymorphism (mtdna rflp ...200516269673
characterization of the novel hch-degrading strain, microbacterium sp. itrc1.a gram-positive microbacterium sp. strain, itrc1, that was able to degrade the persistent and toxic hexachlorocyclohexane (hch) isomers was isolated and characterized. the itrc1 strain has the capacity to degrade all four major isomers of hch present in both liquid cultures and aged contaminated soil. dna fragments corresponding to the two initial genes involved in gamma-hch degradative pathway, encoding enzymes for gamma-pentachlorocyclohexene hydrolytic dehalogenase (linb) and a 2,5-dichloro-2 ...200616315057
symbiotic association in chlorella culture.chlorella sorokiniana iam c-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. in this study, the consortium was separated and five nonalgal constituents, a fungal strain (cssf-1), and four bacterial strains (cssb-1, cssb-2, cssb-3, and cssb-4) were isolated and identified. 16s rdna sequence analysis revealed that strains cssb-1, cssb-2, cssb-3, and cssb-4 were close to ralstonia pickettii (99.8% identity), sphingomonas sp. d ...200516329867
nutritional requirements of microbacterium thermosphactum.microbacterium thermosphactum requires cysteine, alpha-lipoate, nicotinate, pantothenate, p-aminobenzoate, biotin, and thiamin for aerobic growth in glucose-mineral salts medium. glucose cannot be replaced by casamino acids or acids of the tricarboxylic acid cycle as sole carbon and energy sources. the organism can also grow anaerobically in the minimal synthetic medium.197916345460
effect of carbon dioxide on growth of meat spoilage bacteria.the ability of co(2) to inhibit respiration and growth of representative strains of seven species of meat spoilage bacteria was examined. enterobacter and microbacterium thermosphactum were unaffected by co(2). both respiration and growth of the other species were inhibited. with four of the species (fluorescent and nonfluorescent pseudomonas, alteromonas putrefaciens, and yersinia enterocolitica), the inhibition pattern in a complex medium was similar, and inhibition was incomplete and reached ...198016345503
effect of gamma irradiation on the microflora of freshwater fish: ii. generic identification of aerobic bacteria from yellow perch fillets.studies on the generic identification of bacteria isolated from nonirradiated and irradiated (0.3 and 0.6 mrad) yellow perch fillets during the course of microbial spoilage have been conducted. after the enumeration and tabulation of macrocolonies on petri dish cultures obtained from fillets, isolates were examined and keyed out essentially according to modified morphological and biochemical protocols of shewan. identification was further confirmed through reference to bergey's manual. data obta ...196616349702
glycerol ester hydrolase activity of microbacterium thermosphactum.microbacterium thermosphactum possesses a significant glycerol ester hydrolase (lipase, ec activity and a weak but definite carboxylic ester hydrolase (esterase, ec activity. harvested whole cell preparations contained 53 units of lipase activity with tripropionin as the substrate. this activity decreased with an increasing chain length of fatty acid in the triglyceride to 13 units with trilaurin as the substrate and no activity with tripalmitin. maximum lipase activity was fou ...197116349896
bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae).wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ...200616382282
microbacterium koreense sp. nov., from sea water in the south sea of korea.microbacterium strains js53-2t and js53-5 were isolated from sea water in the south sea of korea and subjected to phenotypic, chemotaxonomic and genetic characterization. the cells were found to be gram-positive. these strains contained mk-11 and mk-12 as the main respiratory quinones and anteiso-c15:0, anteiso-c17:0 and iso-c16:0 as the major cellular fatty acids. the dna g+c content was 68 mol%. the cell-wall sugars of the isolates were galactose and xylose, and the diamino acid in the cell-wa ...200616449451
interstitial pulmonary inflammation due to microbacterium sp. after heart transplantation.a coryneform bacterium was isolated from the bronchoalveolar aspirate of a patient with interstitial pulmonary inflammation. commercial systems identified the isolate as corynebacterium sp. or aureobacterium sp./corynebacterium aquaticum, but 16s rrna gene analysis unequivocally attributed it to the genus microbacterium. this represents the first documented case of microbacterium pulmonary infection.200616476799
identifying the bacterial community on the surface of intralox belting in a meat boning room by culture-dependent and culture-independent 16s rdna sequence analysis.we examined the bacterial community present on an intralox conveyor belt system in an operating lamb boning room by sequencing the 16s ribosomal dna (rdna) of bacteria extracted in the presence or absence of cultivation. rflp patterns for 16s rdna clone library and cultures were generated using haeiii and mspi restriction endonucleases. 16s rdna amplicons produced 8 distinct rflp pattern groups. rflp groups i-iv were represented in the clone library and rflp groups i and v-viii were represented ...200616488497
microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest.a xylanolytic bacterium, us15t, was isolated from swamp forest soil in ulsan, korea. the cells of the novel strain were gram-positive, non-motile, short-rod-shaped and showed chemotaxonomic properties that were consistent with its classification in the genus microbacterium. chemotaxonomic results showed mk-12 and mk-11 as major menaquinones, predominating iso- and anteiso-branched cellular fatty acids, glucose, galactose and mannose as cell-wall sugars, peptidoglycan-type b2beta with glycolyl re ...200616514023
the microbacteria ii. : the growth of microbacterium lacticum in a chemically defined medium. 194916561650
planktonic replication is essential for biofilm formation by legionella pneumophila in a complex medium under static and dynamic flow conditions.legionella pneumophila persists for a long time in aquatic habitats, where the bacteria associate with biofilms and replicate within protozoan predators. while l. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clear whether the bacteria form or replicate within biofilms in the absence of protozoa. in this study, we analyzed surface adherence of and biofilm formation by l. pneumophila in a rich medium that supported axenic replication. biofilm formation by t ...200616597995
diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya.we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ...200616598639
survival of surface ripening cultures during storage and monitoring their development on study the survival of bacteria isolated from the surface of smear cheese and monitor their development during cheese ripening.200616599999
the hydantoin transport protein from microbacterium liquefaciens.the gene hyup from microbacterium liquefaciens aj 3912 with an added his6 tag was cloned into the expression plasmid pttq18 in an escherichia coli host strain. the transformed e. coli showed transport of radioisotope-labeled 5-substituted hydantoins with apparent k(m) values in the micromolar range. this activity exhibited a ph optimum of 6.6 and was inhibited by dinitrophenol, indicating the requirement of energy for the transport system. 5-indolyl methyl hydantoin and 5-benzyl hydantoin were t ...200616621827
screening for microbial markers in miocene sediment exposed during open-cast brown coal mining.viable microorganisms were found in miocene lacustrine clays of the cypris formation excavated from 200-m below the surface as spoil during open-cast brown coal mining (sokolov brown coal basin, north-western bohemia, czech republic). both saprotrophic microfungi of the genera penicillium, verticillium, cladosporium and aspergillus as well as heterotrophic bacteria were isolated from an intact sediment cores. heterotrophic bacteria were classified by the mis sherlock system as representatives of ...200616622790
rapid analysis of two food-borne microbial communities at the species level by fourier-transform infrared microspectroscopy.the species composition of microbial communities in natural habitats may be extremely complex and therefore a quantitative analysis of the fraction each species contributes to the consortium has proven to be difficult. during recent years, the identification of bacterial pure cultures based on their infrared spectra has been established. fourier-transform infrared microspectroscopy now proceeds a step further and allows identification of microorganisms directly plated from community dilutions. i ...200616623742
the phylogenetic significance of peptidoglycan types: molecular analysis of the genera microbacterium and aureobacterium based upon sequence comparison of gyrb, rpob, reca and ppk and 16srrna genes.the type strains of 27 species of the genus microbacterium, family microbacteriaceae, were analyzed with respect to the phylogeny of the housekeeping genes coding for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca) and polyphosphate kinase (ppk). the resulting gene trees were compared to the 16s rrna gene phylogeny of the same species. the topology of neighbour-joining and maximum parsimony phylogenetic trees based upon nucleic acid sequences and protein sequen ...200716684595
enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms.most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ...200616751497
[effect of pentachlorophenol (pcp) on bacterial community of an aerobic granular sludge estimated by t-rflp].effect of pentachlorophenol (pcp) on cod and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism (t-rflp) technique. compared with the removal rate of cod, the rate of ammonium removal is more sensitive to pcp. as the ammonium and cod removal rates decreased consistently, the bacterial genera within the aerobic granular sludge also decrease with the increase of pcp conc ...200616768009
genomic clusters, putative pathogen recognition molecules, and antimicrobial genes are induced by infection of c. elegans with m. nematophilum.the interaction between the nematode caenorhabditis elegans and a gram-positive bacterial pathogen, microbacterium nematophilum, provides a model for an innate immune response in nematodes. this pathogen adheres to the rectal and post-anal cuticle of the worm, causing slowed growth, constipation, and a defensive swelling response of rectal hypodermal cells. to explore the genomic responses that the worm activates after pathogenic attack we used microarray analysis of transcriptional changes indu ...200616809667
purification and characterization of two types of chitosanase from a microbacterium sp.two extracellular chitosanases (chix and chin) were extracted from microbacterium sp. ou01 with mr values of 81 kda (chix) and 30 kda (chin). chin was optimally active at ph 6.2 and 50 degrees c and chix at ph 6.6 and 60 degrees c (assayed over 15 min). both the activities increased with the degree of deacetylation (dda) of chitosan. chin hydrolyzed oligomers of glucosamine (glcn) larger than chitopentaose, and chitosan with 62-100% dda; but chix acted on chitosan and released glcn. hydrolysis o ...200616820974
substrate induction and statistical optimization for the production of chitosanase from microbacterium sp. ou01.the chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by microbacterium sp. ou01 in shake-flask. a significant influence of (nh(4))(2)so(4), mgso(4).7h(2)o and initial ph on chitosanase production was noted with plackett-burman design. it was then revealed with the method of steepest ascent and response surface methodology (rsm) that 19.0g/l (nh(4))(2)so(4), 1.3g/l mgso(4) and an initial ph of 2.0 were optim ...200716930999
microbacterium deminutum sp. nov., microbacterium pumilum sp. nov. and microbacterium aoyamense sp. nov.three novel bacterial strains were isolated from a soil sample collected in japan by culture on a gpm agar plate supplemented with superoxide dismutase and catalase. the strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-glycolyl. the major menaquinones were mk-12, 13 and 14. mycolic acids were not detected. g+c contents of the dna were in the range 69-71 mol%. comparative ...200616957107
growth and colour development of some surface ripening bacteria with debaryomyces hansenii on aseptic cheese curd.the growth of five bacteria isolated from red-smear cheeses, brevibacterium aurantiacum, corynebacterium casei, corynebacterium variabile, microbacterium gubbeenense and staphylococcus saprophyticus in mixed cultures with debaryomyces hansenii on aseptic model cheese curd at 10 and 14 degrees c was investigated. at both temperatures, c. casei and micro. gubbeenense had a longer lag phase than c. variabile, brevi. aurantiacum and staph. saprophyticus. in all cultures, lactose was utilised first a ...200616978429
genome characterization, analysis of virulence and transformation of microbacterium nematophilum, a coryneform pathogen of the nematode caenorhabditis elegans.a coryneform bacterium designated microbacterium nematophilum has previously been reported to act as a pathogen for caenorhabditis elegans. this bacterium is able to colonize the rectum of infected worms and cause localized swelling, constipation and slowed growth. additional isolates and analysis of this bacterium are described here. tests of pathogenicity on other caenorhabditis nematodes show that m. nematophilum infection is lethal to most species in the genus, in contrast to its relatively ...200617010162
degradation of environmental endocrine disruptor di-2-ethylhexyl phthalate by a newly discovered bacterium, microbacterium sp. strain this study di-2-ethylhexyl phthalate (dehp)-degradation strain cq0110y was isolated from activated sludge. according to the biophysical/biochemical characteristics and analysis of 16s rdna, the strain was identified as microbacterium sp. the results of this study showed the optimal ph value and optimal temperature which influenced the degradation rate in wastewater: ph 6.5-7.5, 25-35 degrees c. kinetics of degradation reaction had been performed at different initial concentrations and differe ...200717089119
purification and properties of a keratinolytic metalloprotease from microbacterium sp.this study was developed to purify and to characterize a keratinolytic protease from the bacterium microbacterium sp. strain kr10.200617105556
activities and survival of endophytic bacteria in white clover (trifolium repens l.).in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ...200617110977
molecular diversity studies of bacterial communities of oil polluted microbial mats from the etang de berre (france).the biodiversity of microbial mats inhabiting the oil-contaminated lagoon etang de berre was determined by molecular approaches. the fingerprint of denaturing gradient gel electrophoresis (dgge) and automatic ribosomal intergenic spacer analysis (arisa) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. species composition of the mats were compared by constructing 16s rrna libraries. amplified rdna restriction analysi ...200617117996
mos1 mutagenesis reveals a diversity of mechanisms affecting response of caenorhabditis elegans to the bacterial pathogen microbacterium nematophilum.a specific host-pathogen interaction exists between caenorhabditis elegans and the gram-positive bacterium microbacterium nematophilum. this bacterium is able to colonize the rectum of susceptible worms and induces a defensive tail-swelling response in the host. previous mutant screens have identified multiple loci that affect this interaction. some of these loci correspond to known genes, but many bus genes [those with a bacterially unswollen (bus) mutant phenotype] have yet to be cloned. we em ...200717151260
characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria.this study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (eps).200717184342
chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (capsicum anuum l.).a climate chamber experiment was conducted to assay the effect of low temperatures (chilling) on the diversity of bacteria colonizing the endospheres of two thermophilic sweet pepper (capsicum anuum l.) cultivars, milder spiral and ziegenhorn bello. structural diversity was analyzed by 16s rrna-based terminal restriction fragment length polymorphism (t-rflp) analysis and by the generation of 16s rrna gene libraries to determine dominant community members in t-rflp profiles. cultivable community ...200617215894
a new microbacterium species isolated from the blood of a patient with fever: microbacterium pyrexiae sp. nov.a gram-positive bacterium, smc-a8265(t), which was isolated from the blood of a patient with fever but could not be identified by a conventional microbiologic method, was finally characterized by performing phenotypic and genotypic analyses. 16s rrna gene sequence analysis revealed that the strain smc-a8265(t) belonged to the genus microbacterium, but it did not correspond to any of the previously described microbacterium spp. biochemical tests and cellular fatty acid composition analysis also c ...200717240108
identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india.lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ...200717264993
heavy metal resistance and genotypic analysis of metal resistance genes in gram-positive and gram-negative bacteria present in ni-rich serpentine soil and in the rhizosphere of alyssum murale.forty-six bacterial cultures, including one culture collection strain, thirty from the rhizosphere of alyssum murale and fifteen from ni-rich soil, were tested for their ability to tolerate arsenate, cadmium, chromium, zinc, mercury, lead, cobalt, copper, and nickel in their growth medium. the resistance patterns, expressed as minimum inhibitory concentrations, for all cultures to the nine different metal ions were surveyed by using the agar dilution method. a large number of the cultures were r ...200717276484
17beta-estradiol-degrading bacteria isolated from activated sludge.fourteen phylogenetically diverse 17beta-estradiol-degrading bacteria (strains kc1-14) were isolated from activated sludge of a wastewater treatment plant. these isolates widely distributed among eight different genera--aminobacter (strains kc6 and kc7), brevundimonas (strain kc12), escherichia (strain kc13), flavobacterium (strain kc1), microbacterium (strain kc5), nocardioides (strain kc3), rhodococcus (strain kc4), and sphingomonas (strains kc8-kc11 and kc14)--of three phyla: proteobacteria, ...200717310711
caenorhabditis elegans mutants resistant to attachment of yersinia biofilms.the detailed composition and structure of the caenorhabditis elegans surface are unknown. previous genetic studies used antibody or lectin binding to identify srf genes that play roles in surface determination. infection by microbacterium nematophilum identified bus (bacterially unswollen) genes that also affect surface characteristics. we report that biofilms produced by yersinia pestis and y. pseudotuberculosis, which bind the c. elegans surface predominantly on the head, can be used to identi ...200717339204
[evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance].the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ...200617370571
microbacterium isolates from the vicinity of a radioactive waste depository and their interactions with uranium.three oligotrophic bacterial strains were cultured from the ground water of the deep-well monitoring site s15 of the siberian radioactive waste depository tomsk-7, russia. they were affiliated with actinobacteria from the genus microbacterium. the almost fully sequenced 16s rrna genes of two of the isolates, s15-m2 and s15-m5, were identical to those of cultured representatives of the species microbacterium oxydans. the third isolate, s15-m4, shared 99.8% of 16s rrna gene identity with them. the ...200717381522
metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ...200717406774
[reaction of microorganisms to the digestive fluid of the earthworms].the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ...200717410875
culture-independent analysis of bacterial diversity in a child-care facility.child-care facilities appear to provide daily opportunities for exposure and transmission of bacteria and viruses. however, almost nothing is known about the diversity of microbial contamination in daycare facilities or its public health implications. recent culture-independent molecular studies of bacterial diversity in indoor environments have revealed an astonishing diversity of microorganisms, including opportunistic pathogens and many uncultured bacteria. in this study, we used culture and ...200717411442
isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. cv. br29 of bangladesh.a total of 30 bacteria were isolated from the rhizoplane of rice cv. br29 cultivated in mymensingh, bangladesh and from the seedlings obtained from surface-sterilized seeds of br29. upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using national botanical research institute's phosphate medium. the bacterial isolates were identified based on their phenotypic and 16s rrna genes sequencing data as acinetobacter sp. br-12, klebsi ...200717425114
microbacterium terricolae sp. nov., isolated from soil in japan.the taxonomic positions of two novel strains isolated from a soil sample collected in japan using glucose-peptone-meat extract (gpm) agar plates supplemented with superoxide dismutase or superoxide dismutase plus catalase were investigated based on the results of chemotaxonomic, phenotypic and genotypic characteristics. strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-gl ...200717429156
stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese.a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ...200717430918
isolation and characterization of a xanthan-degrading microbacterium sp. strain xt11 from garden soil.isolation and characterization of the xanthan-degrading microbacterium sp. xt11.200717448171
screening and distributing features of bacteria with hydantoinase and carbamoylase.hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ...200917498938
antimicrobial properties of two purified skin peptides from the mink frog (rana septentrionalis) against bacteria isolated from the natural habitat.numerous peptides exhibiting antimicrobial properties have been isolated from the skins of many amphibian species. these peptides offer an innate chemical defense system against various microbial agents that exist in the amphibian's environment. amphibian skin peptides are typically tested for antimicrobial activity against microbial strains that are pathogenic to humans, but not on potential pathogenic or opportunistic bacteria that exist in the organism's habitat. two peptides, a brevinin-2-re ...200717499556
a novel dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of n-acyl amino acids.a novel type of a microbial n-acyl amino acid hydrolase (aah) from insect gut bacteria was purified, cloned and functionally characterized. the enzyme was obtained from microbacterium arborescens se14 isolated from the foregut of larvae of the generalist herbivore spodoptera exigua. the substrates of aah are n-acyl-glutamines previously reported to elicit plant defence reactions after introduction into the leaf during feeding. the isolated aah catalyses the hydrolysis of the amide bond (k(m) = 3 ...200717504494
papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo.fourteen distinct bacterial clones were isolated from surface-sterilized shoot tips (approximately 1 cm) of papaya (carica papaya l. 'surya') planted on murashige and skoog (ms)-based papaya culture medium (23/50 nos.) during the 2-4 week period following in vitro culturing. these isolates were ascribed to six gram-negative genera, namely pantoea (p. ananatis), enterobacter (e. cloacae), brevundimonas (b. aurantiaca), sphingomonas, methylobacterium (m. rhodesianum), and agrobacterium (a. tumefac ...200717538647
gene sequence phylogenies of the family microbacteriaceae.the type strains of 32 species of 13 genera of the family microbacteriaceae were analysed with respect to gene-coding phylogeny for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca), and polyphosphate kinase (ppk). the resulting gene trees were compared with the 16s rrna gene phylogeny of the same strains. the topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differ ...200717551787
effects of ultraviolet radiation on the gram-positive marine bacterium microbacterium maritypicum.although extensive information is available on the effect ultraviolet (uv) radiation has on gram-negative marine bacteria, there is a scarcity of data concerning uv radiation and gram-positive marine bacteria. the focus of this paper is on microbacterium maritypicum, with the gram-negative vibrio natriegens being used as a standard of comparison. m. maritypicum exhibited growth over a nacl range of 0-1000 mm: , with optimum growth occurring between 0 and 400 mm: nacl. in contrast, v. natriegens ...200717551790
molecular cloning, expression and characterization of a chitosanase from microbacterium sp.a gene encoding a chitosanase (mschito) was cloned from microbacterium sp. ou01. the orf consists of 801 bp which encoded a polypeptide of 266 amino acid residues. the deduced amino acid sequence shows 98% identity to that of the chitosanase reported in pseudomonas sp. a-01. in addition, the fusion protein containing mschito was expressed in e. coli and purified using ni-nta affinity chromatography. the purified rmschito protein degraded the chitosan (the degree of deacetylation of 99%) and prod ...200717563859
microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated this study we evaluated the capacity of a defined microbial consortium (five bacteria: mycobacterium fortuitum, bacillus cereus, microbacterium sp., gordonia polyisoprenivorans, microbacteriaceae bacterium, naphthalene-utilizing bacterium; and a fungus identified as fusarium oxysporum) isolated from a pahs contaminated landfarm site to degrade and mineralize different concentrations (0, 250, 500 and 1000 mg kg(-1)) of anthracene, phenanthrene and pyrene in soil. pahs degradation and mineraliz ...200817572084
purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents, from dimethylsulfoxide-tolerant brachybacterium sp. strain lb25.a bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (dmso) was isolated and identified as brachybacterium sp. strain lb25. the amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kda. the enzyme was stable at ph 7.0-8.5 and active at ph 6.0-7.5. the optimum temperature at ph 7.0 was 35 degrees c in the presence of 5 mm cacl(2). the enzyme hydrolyzed starch to produce maltotriose primari ...200717619813
[isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis].five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. they were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. strain phd-2, phd-4 and phd-5 belonged to the genera of ralstonia, acinetobacter and microbacterium respectively; strain phd-1 and phd-3 were from the genus of pseudomonas. homology compari ...200717633645
isolation of bacterial endophytes from germinated maize kernels.the germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. bacterial strains identified by partial 16s rrna gene sequencing as pantoea sp., microbacterium sp., frigoribacterium sp., bacillus sp., paenibacillus sp., and sphingomonas sp. were isolated from kernels of 4 different maize cultivars. genus pantoea was associated with a specific maize cultivar. the kernels of this cultivar were ofte ...200717668041
microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the chagos trench, indian ocean.two bacterial strains, bbh6(t) and bbh9, were isolated from a deep-sea sediment sample collected from the chagos trench, indian ocean, at a depth of 5904 m. the two strains were closely related in their 16s rrna gene sequences (99.7 %), belonged to one genomic species and were virtually identical at the phenotypic level. microbacterium barkeri dsm 20145(t) was the nearest phylogenetic neighbour to the new isolates, with 16s rrna gene sequence similarity levels of 97.2-97.4 %. the new isolates ex ...200717684264
orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin.germination of orchid seeds is a complex process. in this paper we focus on interactions between the host-plant and its bacterial partners via indole-3-acetic acid (iaa). originally isolated from the roots of the epiphytic orchid dendrobium moschatum, the strains of rhizobium, microbacterium, sphingomonas, and mycobacterium genera were among the most active iaa producers. addition of exogenous tryptophan significantly enhanced auxin formation both in mineral and complex media. the presence of ia ...200717687544
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...200717701035
alkaliphilic and halophilic hydrocarbon-utilizing bacteria from kuwaiti coasts of the arabian animate materials from the intertidal zone of the arabian gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. the alkaliphilic oil-utilizing bacteria, as identified by their 16s ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: marinobacter, micrococcus, dietzia, bacillus, oceanobacillus, and citricoccus. the halophilic oil-utilizing bacteria belonged to the genera: marinobacter, georgenia, microbacterium ...200717710391
characterization of predominant bacteria isolates from clean rooms in a pharmaceutical production screen for the predominant bacteria strains distributed in clean rooms and to analyze their phylogenetic relationships.200717726748
conversion of major ginsenoside rb1 to 20(s)-ginsenoside rg3 by microbacterium sp. gs514.ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological activities. many studies have been directed towards converting major ginsenosides to the more active minor ginsenoside, rg3. due to the difficulty in preparing ginsenoside rg3 enzymatically, the compound has been mainly produced by either acid treatment or heating. a microbial strain gs514 was isolated from soil around ginseng roots in a field and used for enzymatic preparation of the ginsenoside rg3 ...200817764709
vitamin requirements of microbacterium lacticum orla-jensen. 194717797253
diversity of microorganisms in decaying maize stalks revealed by a molecular method.microbial diversity in decaying maize stalk was characterized by constructing and analyzing rrna gene clone library. total 47 otus were obtained from 82 bacterial clones, including proteobacteria (64.6%), actinobacteria (30.5%), bacteroidetes (2.4%) and firmicutes (2.4%). most proteobacterial clones were members of rhizobium, pseudomonas and stenotrophomonas. eighty-four percent of actinobacteria was related to microbacterium. only 14 otus were identified from 124 fungal clones, including ascomy ...200717846593
isolation, characterization and complete nucleotide sequence of a novel temperate bacteriophage min1, isolated from the nematode pathogen microbacterium nematophilum.we report the discovery, properties and complete sequence (46,365bp) of min1, the first bacteriophage to be reported for the coryneform genus microbacterium. this temperate phage is normally integrated into a stable plasmid, pmn1, found in cells of microbacterium nematophilum, a pathogen of certain soil nematodes including caenorhabditis elegans, but it can also grow lytically. the phage is lambdoid in morphology and in sequence, belonging to the family siphoviridae. general and specific feature ...200717869067
performance and microbial diversity of a membrane bioreactor treating real textile dyeing wastewater.the textile industry is one of the major industries of taiwan but unfortunately it produces toxic and low biodegradable wastewater. to remedy this problem, this study compared the performance of the membrane bioreactor (mbr) and sequencing batch reactor (sbr) processes for treating real textile dyeing wastewater. the microbial diversity of the mbr process was also identified by a combination of culturing methods and molecular biotechnology. the removal efficiencies of the mbr process for color, ...200717879852
adsorption of microorganisms onto an artificial siderophore-modified au substrate.the hydroxamate-type artificial siderophore, tris[2-{3-(n-acetyl-n-hydroxamino)propylamido}propyl]aminomethane (tappa) and its fe(iii) complex, fe(iii)-tappa were prepared and characterized by several spectroscopic methods. fe(iii)-tappa exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, microbacterium flavescens, suggesting that fe(iii)-tappa can permeate the cell membrane of the microorganism. the adsorption of the fe(iii)-siderophore complex onto a de ...200717890077
culturable psychrotrophic bacterial communities in raw milk and their proteolytic and lipolytic traits.during cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. the diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. about 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in ...200717890340
diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates.bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ...200717905875
microbacterium sediminicola sp. nov. and microbacterium marinilacus sp. nov., isolated from marine environments.two novel microbacterium species are described on the basis of phenotypic, chemotaxonomic and genotypic studies. the two strains, designated ym10-847(t) and ym11-607(t), were isolated from river sediment and unidentified hydroid, respectively, of a marine lake. the strains were gram-positive, catalase-positive bacteria with l-ornithine as the diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-glycolyl. the major menaquinones were mk-10 and mk-11 for ym10-847(t ...200717911310
growth characteristics of brevibacterium, corynebacterium, microbacterium, and staphylococcus spp. isolated from surface-ripened cheese.the growth characteristics of five bacteria, brevibacterium aurantiacum 1-16-58, corynebacterium casei dpc 5298(t), corynebacterium variabile dpc 5310, microbacterium gubbeenense dpc 5286(t), and staphylococcus saprophyticus 4e61, all of which were isolated from the surface of smear cheese, were studied in complex and chemically defined media. all of the coryneforms, except m. gubbeenense, grew in 12% salt, while b. aurantiacum and s. saprophyticus grew in 15% salt. all five bacteria assimilated ...200717921266
rheological characterization of levan polysaccharides from microbacterium laevaniformans.levan polysaccharides were produced from microbacterium laevaniformans and its rheological behaviors were characterized as a function of concentration and temperature. the intrinsic viscosity of the purified levan was determined to be 0.38dl/g at 25 degrees c which was relatively higher than that of levans from other microbial sources. the flow behaviors of the levan solutions were characterized by the increase in the shear stress, giving more increments in the shear rate. thus, the levan soluti ...200817931701
interactions with microbial pathogens.a wide variety of bacterial pathogens, as well as several fungi, kill c. elegans or produce non-lethal disease symptoms. this allows the nematode to be used as a simple, tractable model host for infectious disease. human pathogens that affect c. elegans include gram-negative bacteria of genera burkholderia, pseudomonas, salmonella, serratia and yersinia; gram-positive bacteria enterococcus, staphylococcus and streptococcus; and the fungus cryptococcus neoformans. microbes that are not pathogenic ...200518050390
degradation of phenanthrene on plant leaves by phyllosphere bacteria.the activity of phyllosphere bacteria in the degradation of phenanthrene was investigated as a mechanism for the removal of atmospheric phenanthrene after its deposition on plant leaves. initially, leaf samples of six plant species were collected from two roadsides in bangkok to determine the presence of phenanthrene-degrading bacteria. the numbers of phenanthrene-degrading phyllosphere bacteria were varied and ranged from 3.5 x 10(4) to 1.95 x 10(7) cfu/g, in which the highest number was found ...200718057816
sugar beet-associated bacterial and fungal communities show a high indigenous antagonistic potential against plant pathogens.the aim of this study was to analyze microbial communities in/on sugar beet with special focus on antagonists toward plant pathogens. for this purpose, the composition of microorganisms isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field-grown sugar beet plants was analyzed by a multiphasic approach at three different plant development stages at six locations in europe. the analysis of microbial communities by single strand conformation polymorphism (sscp) of 16s/18s ...200818060449
[microbial diversity in scorpion intestine (buthus martensii karsch)].scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ...200718062268
effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation.spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ...200818083857
the growth of microbacterium lacticum in a chemically defined medium. 194918108881
infections caused by coryneform bacteria in pediatric oncology patients.invasive infections caused by coryneform bacteria are uncommon but have been reported with increasing frequency in recent decades, especially in immunocompromised persons. because pediatric experience is limited, we examined the epidemiology and clinical characteristics of these infections in children undergoing cancer therapy.200818174873
a novel recombinant system for functional expression of myonecrotic snake phospholipase a(2) in escherichia coli using a new fusion affinity tag.a novel recombinant expression system in escherichia coli was developed using conger eel galectin, namely, congerin ii, as an affinity tag. this system was applied for the functional expression of myotoxic lysine-49-phospholipase a(2) ([lys(49)]pla(2)), termed bpii and obtained from protobothrops flavoviridis (pf) venom. recombinant pf bpii fused with a congerin tag has been successfully expressed as a soluble fraction and showed better quantitative yield when folded correctly. the solubility of ...200818207418
microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ...200818218944
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