purification and properties of pyridoxine oxidase from aureobacterium luteolum and pyridoxal reductase from schizosaccharomyces pombe. 200010705982
identification of coryneform bacterial isolates by ribosomal dna sequence analysis.identification of coryneform bacteria to the species level is important in certain circumstances for differentiating contamination and/or colonization from infection, which influences decisions regarding clinical intervention. however, methods currently used in clinical microbiology laboratories for the species identification of coryneform bacteria are often inadequate. we evaluated the microseq 500 16s bacterial sequencing kit (perkin-elmer biosystems, foster city, calif.), which is designed to ...200010747168
high resistance to oxygen radicals and heat is caused by a galactoglycerolipid in microbacterium sp. m874.microbacterium sp. m874 produced a glyceroglycolipid, di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol, at about the 50 microm level. though the strain was highly resistant to tertiary-butyl hydroperoxide (tbhp) in a glycolipid-productive medium, the resistance was reduced in a nonproductive medium. exogenous addition of the glycolipid to the nonproductive culture restored the resistance. this addition also increased the resistance to heat, ethanol, and 4-chloro-1-naphthol, i ...200010788780
rescuing activity of galactoglycerolipids from cellular lesions induced by 5-aminolevulinic anti-oxygen radical reagent of a bacterial metabolite, m874 monogalactoglycerolipid (di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol ), was tested for its ability to protect two organisms against cellular lesions induced by 5-aminolevulinic acid (ala) and light. in corynebacterium flavescens atcc 10340, extracellular uroporphyrin and coproporphyrin were the main porphyrin products. although less than 2 mm ala increased porphyrin synthesis, ala levels above 3 mm inhibited ...200010788790
production and structure elucidation of glycoglycerolipids from a marine sponge-associated microbacterium species.the bacterium microbacterium sp., isolated from the sponge halichondria panicea, produced four unusual cell-associated glycoglycerolipids and one diphosphatidylglycerol when grown on marine broth and on artificial seawater media. the lipids were isolated by chromatography on silica columns and their structures elucidated using a combination of multidimensional nmr and ms techniques. the main compound was 1-o-acyl-3-[alpha-glucopyranosyl-(1-3)-(6-o-acyl-alpha-mannopyranosyl )]glycerol (ggl.2) wit ...200010843572
comparison of colony lift with direct spotting methods of blot preparation on the effect of colony hybridization in the detection of environmental organisms.nucleic acid probes are used on site to detect or to identify individual microbial cells without cultivation. this molecular technique can avoid some limitations of traditional identification methods including time consuming and imprecise. this study examined the factors affecting colony hybridization and compared the effectiveness of membrane prepared by colony lifting with direct spotting procedures using the universal probe eub 338. the results of hybridization varied depending on the type of ...200010917884
overexpression, purification, and characterization of bacillus subtilis n-acetylmuramoyl-l-alanine amidase cwlc.n-acetylmuramoyl-l-alanine amidase cwlc of bacillus subtilis was overproduced in escherichia coli and purified 21-fold. the amidase hydrolyzed type a cell walls such as b. subtilis. the amidase bound slightly to the microbacterium lacticum cell wall (type b), but did not entirely hydrolyze it. the presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation.200010945275
microbial diversity during maturation and natural processing of coffee cherries of coffea arabica in brazil.the magnitude and diversity of the microbial population associated with dry (natural) processing of coffee (coffea arabica) has been assessed during a 2-year period on 15 different farms in the sul de minas region of brazil. peptone water-washed samples were taken of maturing cherries on trees (cherries, raisins and dried cherries) and from ground fermentations. the microbial load varied from 3 x 10(4) to 2.2 x 10(9) cfu/cherry with a median value of 1.6 x 10(7) cfu/cherry. the microbial load in ...200011016614
identification of corynebacterium bovis and other coryneforms isolated from bovine mammary glands.bovine mastitis remains the most economically important disease in dairy cows. corynebacterium bovis, a lipid-requiring corynebacterium spp., is frequently isolated from the milk of infected mammary glands of dairy cows and is associated with reduced milk production. a total of 212 coryneform bacteria isolated from the milk of dairy cows were obtained from mastitis reference laboratories in the united states and canada. all isolates had been presumptively identified as corynebacterium bovis base ...200011049082
a novel bacterial pathogen, microbacterium nematophilum, induces morphological change in the nematode c. elegans.the dar (deformed anal region) phenotype, characterized by a distinctive swollen tail, was first detected in a variant strain of caenorhabditis elegans which appeared spontaneously in 1986 during routine genetic crosses [1,2]. dar isolates were initially analysed as morphological mutants, but we report here that two independent isolates carry an unusual bacterial infection different from those previously described [3], which is the cause of the dar phenotype. the infectious agent is a new specie ...200011137017
enumeration and characterization of cellulolytic bacteria from refuse of a landfill.enumeration and phenotypic characterization of aerobic cellulolytic bacteria were performed on fresh, 1 year old and 5 years old refuse samples of a french landfill site. numbers of cellulolytic bacteria ranged from 1.1x10(6) to 2.3x10(8) c.f.u. (g dry wt.)(-1) and were lower in 5 years old refuse samples. a numerical analysis of phenotypic data based on 80 biochemical tests and performed on 321 gram-positive isolates from refuse, revealed a high phenotypic diversity of cellulolytic bacteria whi ...200111137603
resident aerobic microbiota of the adult human nasal cavity.recent evidence strongly suggests that the microbiota of the nasal cavity plays a crucial role in determining the reaction patterns of the mucosal and systemic immune system. however, little is known about the normal microbiota of the nasal cavity. the purpose of this study was to determine the microbiota in different parts of the nasal cavity and to develop and evaluate methods for this purpose. samples were collected from 10 healthy adults by nasal washes and by swabbing of the mucosa through ...200011200821
siderophore mediated plutonium accumulation by microbacterium flavescens (jg-9).uptake of plutonium and uranium mediated by the siderophore desferrioxamine-b (dfob) has been studied for the common soil aerobe microbacterium flavescens(jg-9). m. flavescens does not bind or take up nitrilotriacetic acid (nta) complexes of u(vi), fe(iii), or pu(iv) or u(vi)-dfob but does take up fe(iii)-dfob and pu(iv)-dfob. pu(iv)-dfob and fe(iii)-dfob accumulations are similar: only living and metabolically active bacteria take up these metal-siderophore complexes. the fe(iii)-dfob and pu(iv ...200111478246
description of microbacterium foliorum sp. nov. and microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of aureobacterium resistens (funke et al. 1998) as microbacterium resistens comb. nov..the taxonomic position of a group of coryneform bacteria isolated from the phyllosphere of grasses and the surface litter after sward mulching was investigated. on the basis of restriction analyses of 16s rdna, the isolates were divided into two genotypes. according to the 16s rdna sequence analysis, representatives of both genotypes were related at a level of 99.2% similarity and clustered within the genus microbacterium. chemotaxonomic features (major menaquinones mk-12, mk-11 and mk-10; predo ...200111491322
nosocomial outbreak of microbacterium species bacteremia among cancer date, only 6 sporadic microbacterium species (formerly coryneform centers for disease control and prevention [cdc] groups a-4 and a-5) infections have been reported. the source, mode of transmission, morbidity, mortality, and potential for nosocomial transmission of microbacterium species remain unknown. from 26 july through 14 august 1997, 8 episodes of coryneform cdc group a-5 symptomatic bacteremia occurred in 6 patients on the oncology ward at the maine medical center. one patient died. a ...200111517437
molecular and enzymatic characterization of a levan fructotransferase from microbacterium sp. al-210.microbacterium sp. al-210 producing a novel levan fructotransferase (lftase) was screened from soil samples. the lftase was purified to homogeneity by (nh4)2so4 fractionation, column chromatography on resource q, and superdex 200hr. the molecular weight of the purified enzyme was estimated to be approximately 46 kda by both sds-page and gel filtration, and the enzyme's isoelectric point was ph 4.8. the major product produced from the levan hydrolysis by the enzyme reaction was identified by atmo ...200111522362
chromate reduction and 16s rrna identification of bacteria isolated from a cr(vi)-contaminated site.a gram-positive, hexavalent chromium [chromate: cr(vi)]-tolerant bacterium, isolated from tannery waste from pakistan, was identified as a microbacterium sp. by 16s rrna gene sequence homology. the strain (designated as mp30) reduced toxic cr(vi) only under anaerobic conditions at the expense of acetate as the electron donor. the bacterium was able to grow aerobically in l-broth supplemented with 15 mm cro4(2-) but then did not reduce cr(vi). at a concentration of 2.4x10(9) cells/ml, 100 microm ...200111693930
microbacterium gubbeenense sp. nov., from the surface of a smear-ripened cheese.phenotypic and phylogenetic studies were performed on 11 strains of a microbacterium-like organism isolated from the surface of a smear-ripened cheese. the isolates were gram-positive, catalase-positive, facultatively anaerobic, oxidase-negative, non-spore-forming, non-motile, small, slender rods and grew in 12% (w/v) nacl. chemotaxonomic investigation revealed that all the isolates belonged unambiguously to the genus microbacterium. they contained type b1 peptidoglycans with l-lysine as the dia ...200111760936
purification and characterization of a novel esterase promising for the production of useful compounds from microbacterium sp. 7-1w.a novel esterase catalyzing regioselective hydrolysis was purified from the membrane fraction of microbacterium sp. 7-1w, and characterized. the enzyme was solubilized with brij 58 and purified 13.8-fold to apparent homogeneity with 2.58% overall recovery. the relative molecular mass of the native enzyme as estimated by gel filtration was more than 600,000 da, and the subunit molecular mass was 62,000 da. the enzyme catalyzed cleavage of the terminal ester bonds of cetraxate esters and pantothen ...200211814667
biodiversity of the bacterial flora on the surface of a smear cheese.the bacteria on the surface of a farmhouse smear-ripened cheese at four stages of ripening (4, 16, 23, and 37 days) from inoculated (i.e., deliberately inoculated with brevibacterium linens bl2) and noninoculated (not deliberately inoculated with b. linens bl2) cheese were investigated. the results show that, contrary to accepted belief, b. linens is not a significant member of the surface flora of smear cheese and no microbial succession of species occurred during the ripening of the cheeses. o ...200211823224
selective enrichment and characterization of a phosphorus-removing bacterial consortium from activated sludge.under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. the phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. the addition of calcium and magnesium salts, as well as vitamins and trace elements, to autoclaved sterile filtrate of activated sludge was not sufficient to achieve stable phosphorus removal ...200211831470
aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis.200211849325
arsenic (iii) oxidizing microbacterium lacticum and its use in the treatment of arsenic contaminated develop a microbially-assisted process for the removal of arsenic from contaminated groundwater.200211940155
isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants.endophytic bacteria reside within plant hosts without causing disease symptoms. in this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. we determined several phenotypic properties and found approximately equal numbers of gram-negative and gram-positive isolates. in a greenhouse study, 28 of 86 prairie plant endophytes were found to colonize their original hosts at 42 days postinoculation at levels of 3.5 to 7.7 log(10) ...200211976089
purification and characterization of pyridoxal 4-dehydrogenase from aureobacterium luteolum.a pyridoxal dehydrogenase was purified to homogeneity from aureobacterium luteolum, which can use pyridoxine as a carbon and nitrogen source, and characterized. the enzyme was a dimeric protein with a subunit molecular weight of 38,000. it had several properties distinct from those of the partially purified enzyme from pseudomonas ma-1. the optimum ph (8.0-8.5) was 0.8-1.3 lower than that of the pseudomonas enzyme. the aureobacterium enzyme showed much higher and lower affinities for nad+ (km, 0 ...200212005047
prevalent bacterial species and novel phylotypes in advanced noma lesions.the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ...200212037085
expression, purification and characterization of a recombinant levan fructotransferase.a 1.6 kb dna fragment including the lftm gene, encoding a levan fructotransferase (lftase) of microbacterium sp. al-210, was subcloned into a high-expression vector, pet-29b, and the recombinant enzyme was overexpressed in escherichia coli. most of the lftase activity was detected in the cytoplasmic fraction after induction with isopropyl beta-d-thiogalactoside. the recombinant enzyme with a tag of six histidine residues at the c-terminus was purified 132-fold by affinity and gel-filtration chro ...200212074698
catheter-related microbacterium bacteremia identified by 16s rrna gene sequencing.we describe the application of 16s rrna gene sequencing in defining two cases of catheter-related microbacterium bacteremia. in the first case, a gram-positive bacillus was isolated from both the blood culture and central catheter tip of a 39-year-old woman with chronic myeloid leukemia. the api coryne system identified the isolate as 98.9% aureobacterium or corynebacterium aquaticum. in the second case, a gram-positive bacillus was recovered from five sets of blood cultures from both central ca ...200212089308
purification, molecular cloning, and characterization of pyridoxine 4-oxidase from microbacterium luteolum.pyridoxine 4-oxidase (ec, pn 4-oxidase), which catalyzes the oxidation of pn by oxygen or other hydrogen acceptors to form pyridoxal and hydrogen peroxide or reduced forms of the acceptors, respectively, was purified for the first time to homogeneity from microbacterium luteolum yk-1 (=aureobacterium luteolum yk-1). the purified enzyme required fad for its catalytic activity and stability. the enzyme was a monomeric protein with the subunit molecular mass of 53,000 +/- 1,000 da. pn was ...200212092811
cloning and characterization of a levanbiohydrolase from microbacterium laevaniformans atcc extracellular levanbiohydrolase gene, levm, from microbacterium laevaniformans atcc 15953 was cloned and its nucleotide sequence was determined. nucleotide sequence analysis of this gene revealed a 1863 bp open reading frame coding for a protein of 621 amino acids. the deduced amino acid sequence of the levm gene exhibited 28-47% sequence identities with levanases, levanfructotransferases, and inulinases. the levm was overexpressed by using a t7 promoter in escherichia coli bl21 (de3) and pur ...200212095678
plasmid-borne genes code for an angular dioxygenase involved in dibenzofuran degradation by terrabacter sp. strain yk3.the genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of pcr primers designed by using conserved sequences of the dioxygenase alpha subunit genes. one sequence of alpha subunit genes was commonly amplified from four dibenzofuran-utilizing actinomycetes: terrabacter sp. strains yk1 and yk3, rhodococcus sp. strain yk2, and microbacterium sp. strain yk18. a 5.2-kb psti fragment encoding the alpha and beta subunits of the terminal diox ...200212147464
microbacterium aerolatum sp. nov., isolated from the air in the 'virgilkapelle' in vienna.three rod-shaped, gram-positive strains were isolated from the air of the chapel 'virgilkapelle' in vienna. a representative of these three strains, strain v-73t, shared the highest 16s rdna sequence similarities with members of the genus microbacterium, in particular microbacterium foliorum, microbacterium testaceum, microbacterium esteraromaticum, microbacterium keratanolyticum and microbacterium arabinogalactanolyticum. the strains displayed almost identical biochemical and physiological char ...200212148633
microbiology of acute otitis isolate and characterize bacteria and fungi from acute otitis externa (aoe) and to obtain susceptibility profiles on each bacterial isolate.200212169893
isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading rhodococcus species.sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including rhodococcus, microbacterium, and terrabacter genera. heretofore, no dibenzofuran-utilizing strain belonging to the genus microbacterium has been reported. five extradiol dioxygenase genes (dfdb, and edil to 4) of the strain rhodococcus sp. yk2 were cloned and analyzed. the nucleotide sequence of dfdb gene was almost identical to the bphc1 gene of terrabacter sp. dpo360, which wa ...200212224629
microbial growth-promotion activity of 3-hydroxymonoazine- and n-hydroxydiazine-type heterocycles.three 3-hydroxymonoazine- and three n-hydroxydiazine-type heterocycles were tested whether they act as artificial siderophores toward aureobacterium flavescens jg-9 (atcc no. 25091). among them, 1-hydroxy-3,5,6-trimethyl-2(1h)-pyrazinone (3) showed the highest growth-promotion activity comparable to desferrioxamine b (dfb), a natural trihydroxamate siderophore, at 48.5 microm or above, followed by 1-hydroxy-5,6-dimethyl-2(1h)-pyrazinone (2), 1-hydroxy-4,6-dimethyl-2(1h)-pyrimidinone (1), and 3-h ...200212235861
isolation and characterization of efficient isoxaben-transforming microbacterium sp strains from four european soils.nutrient-agar plates containing isoxaben (500 mg litre(-1)) were used to isolate isoxaben-metabolising bacteria from four european soils incubated with the herbicide under laboratory conditions. in flask experiments, inoculation of a basal salts medium containing nitrogen and [phenyl-u-14c]isoxaben with an isolate (b2b) resulted in 33% recovery of the initial radioactivity as [14c]carbon dioxide after 2 weeks. a major metabolite identified by gc-ms and nmr analysis as 3-(1-ethyl-1-methylpropyl)i ...200212476996
exposure to airborne microorganisms and endotoxin in a potato processing plant.microbiological air sampling was performed in a big potato processing plant located in eastern poland. air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in the division producing potato flakes and meal from dried potato pulp and at 2 sites in the division producing potato syrup from imported starch. the concentrations of total airborne microorganisms were within a range of 28.3-93.1 x 10(3) cfu/m(3). mesophilic bacteria were dominant ...200212498592
investigations on rhizoplane actinobacteria communities of papyrus (cyperus papyrus) from an egyptian wetland.wetlands have important global ecological functions, which include carbon storage and water interception. wetland contributes to the maintenance of regional and global biodiversity. though many important wetland ecological functions are based on microbial metabolism, we have scanty knowledge on microbial diversity in wetlands. plant rhizoplane habitats are considered to harbor highly diverse bacterial communities. most of the floating mats on river nile are dominated by papyrus (cyperus papyrus) ...200212512252
initial characterization of new bacteria degrading high-molecular weight polycyclic aromatic hydrocarbons isolated from a 2-year enrichment in a two-liquid-phase culture characterize some polycyclic aromatic hydrocarbons (pah)-degrading microorganisms isolated from an enriched consortium degrading high molecular weight (hmw) pahs in a two-liquid-phase (tlp) soil slurry bioreactor, and to determine the effect of low molecular weight (lmw) pah on their growth and hmw pah-degrading activity.200312534823
diversity of 746 heterotrophic bacteria isolated from microbial mats from ten antarctic lakes.microbial mats, growing in antarctic lakes constitute unique and very diverse habitats. in these mats microorganisms are confronted with extreme life conditions. we isolated 746 bacterial strains from mats collected from ten lakes in the dry valleys (lakes hoare and fryxell), the vestfold hills (lakes ace, druzhby, grace, highway, pendant, organic and watts) and the larsemann hills (lake reid), using heterotrophic growth conditions. these strains were investigated by fatty acid analysis, and by ...200212583721
molecular cloning of the gene for 2,6-beta-d-fructan 6-levanbiohydrolase from streptomyces exfoliatus f3-2.the gene encoding a 2,6-beta-d-fructan 6-levanbiohydrolase (lf2ase) (ec that converts levan into levanbiose was cloned from the genomic dna of streptomyces exfoliatus f3-2. the gene encoded a signal peptide of 37 amino acids and a mature protein of 482 amino acids with a total length of 1560 bp and was successfully expressed in escherichia coli. the similarities of primary structure were observed with levanases from clostridium acetobutylicum, bacillus subtilis, b. stearothermophilus ( ...200312586402
biocatalytic deprotection of a cetraxate ester by microbacterium sp. strain 7-1w cells.enzymatic deprotection of the terminal ester bond of a cetraxate methyl ester was done with resting cells of microbacterium sp. strain 7-1w, which produces an esterase catalyzing a regioselective hydrolysis reaction, as the catalyst. when 20 g of cetraxate methyl ester in 50 ml of a reaction mixture was incubated with 5 g of wet cells for 17 h, 96% of the substrate was converted to the desired product, cetraxate, quantitatively.200312619695
microbial contamination of non-disposable instruments in otolaryngology out-patients.nosocomial infections are an important cause of morbidity and contaminated equipment may contribute to this. there has been little work concerning contamination of non-disposable equipment in otolaryngology. this study investigates the efficacy of the cleaning regimen for non-disposable instruments in an otolaryngology out-patients clinic. instruments were swabbed before each clinic, when they had been autoclaved, then after use on patients, when they had been washed with detergent and chlorhexi ...200312625885
bacteremia due to a novel microbacterium species in a patient with leukemia and description of microbacterium paraoxydans sp. nov.a yellow-pigmented coryneform rod was isolated from the blood of a child with acute lymphoblastic leukemia who was perfused with a central venous catheter. the culture bottles were positive twice, at a 2-month interval. the isolate was identified as a microbacterium sp. and studied along with five other similar strains. phenotypic, chemotaxonomic, and genetic characteristics indicated that they are closely related to microbacterium oxydans but that they belong to a distinct species, for which th ...200312734292
sediment microbes of deep-sea bioherms on the northwest shelf of australia.the northwest shelf of australia is a region with known petroleum reserves. recent geological investigations have revealed carbonate knolls postulated to be of biological origin. sediment microbial populations at three sites on the northwest shelf, pee shoal, mermaid reef, and scott reef, were investigated for the presence of hydrocarbon-degrading bacteria. from two sampling trips, 246 bacterial strains were purified, and 182 of these strains displayed preferential growth on hydrocarbon-selectiv ...200312739077
contamination of bone marrow products with an actinomycete resembling microbacterium species and reinfusion into autologous stem cell and bone marrow transplant recipients.bacterial contamination of bone marrow or peripheral blood stem cell transplant products typically occurs with skin flora or, rarely, gram-negative organisms. we describe a clonal outbreak of contamination in transplant products caused by contamination with an aerobic actinomycete that occurred at our institution during the summer of 2001. from 1 july through 12 september 2001, 73 peripheral blood or bone marrow stem cell products were obtained from 39 patients, and 34 products were found to be ...200312746790
development and application of an assay for uranyl complexation by fungal metabolites, including assay to detect uo(2)(2+) complexation was developed based on the chrome azurol s (cas) assay for siderophores (b. schwyn and j. b. neilands, anal. biochem. 160:47-56, 1987) and was used to investigate the ability of fungal metabolites to complex actinides. in this assay the discoloration of two dyed agars (one containing a cas-fe(3+) dye and the other containing a cas-uo(2)(2+) dye) caused by ligands was quantified. the assay was tested by using the siderophore desferrioxamine b (dfo), and t ...200312788768
pair-dependent co-aggregation behavior of non-flocculating sludge bacteria.two strains of non-flocculating sewage sludge bacteria (xanthomonas sp. s53 and microbacterium esteraromaticum s51) showed 91% and 77% co-aggregation, respectively, with acinetobacter johnsonii s35 using a spectrophometric assay. the co-aggregates in case of xanthomonas sp. s53 and a. johnsonii s35 were above 100 microm and stable against edta (2 mm) and a commercial protease (0.2 mg ml(-1)). protease/periodate pretreatment of the partners did not affect this co-aggregation. on the other hand, c ...200312889835
a microbiological assay for isonicotinyl hydrazine. 195212983360
some studies on microbacteria from iowa dairy products. 195313008430
evidence for an unidentified growth factor required by microbacterium flavum. 195213027321
microbacterium thermosphactum, spec: nov.; a nonheat resistant bacterium from fresh pork sausage. 195313069397
the type of lactic acid formed by microbacterium. 195413163018
the pathways of glucose dissimilation by microbacterium lacticum. 195613319250
a thermophilic, motile strain of microbacterium. 195713509641
pathway of ribose 5-phosphate utilization in microbacterium lacticum. 196113754316
[comparative evaluation of microbiological application of phase and anoptral methods of contrasting]. 195913813621
'iron transport' compounds as growth stimulators for microbacterium sp. 195913815776
heat resistance in corynebacterium and the relationship of the genus to microbacterium. 195514381368
an absolute requirement for 'iron transport factors' by microbacterium lacticum 8181. 195914401003
[virulence of acid-fast bacteria coated with oil or fat, with special reference to an observation on the so-called atypical acid-fast bacteria. 2. subcutaneous and intraperitoneal inoculation with m. phlei coated with guineapig fat]. 196214460202
identification and differentiation of species and strains of arthrobacter and microbacterium barkeri isolated from smear cheeses with amplified ribosmal dna restriction analysis (ardra) and pulsed field gel electrophoresis (pfge).ardra (amplified ribosomal-dna restriction analysis) was used to differentiate among species and genera of arthrobacter and microbacteria. species-specific restriction patterns of pcr-products were obtained with ncii for arthrobacter citreus (dsm 20133t), a. sulfureus (dsm 20167t), a. globiformis (dsm 20124t) and a. nicotianae strains (dsm 20123t, mge 10d, ca13, ca14, isolate 95293, 95294, and 95299), a. rhombi ccug 38813t, and ccug 38812, and microbacterium barkeri strains (dsm 30123t, mge 10d, ...200314529187
coaggregation among nonflocculating bacteria isolated from activated sludge.thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. among these strains, eight showed significant (74 to 99%) coaggregation with acinetobacter johnsonii s35 while only four strains coaggregated, to a lesser extent (43 to 65%), with acinetobacter junii s33. the extent and pattern of coaggregation as well as the aggregate size showed good correlation w ...200314532062
intrinsic bioremediability of an aromatic hydrocarbon-polluted groundwater: diversity of bacterial population and toluene monoxygenase genes.the functional and phylogenetic biodiversity of bacterial communities in a benzene, toluene, ethylbenzene and xylene (btex)-polluted groundwater was analysed. to evaluate the feasibility of using an air sparging treatment to enhance bacterial degradative capabilities, the presence of degrading microorganisms was monitored. the amplification of gene fragments corresponding to toluene monooxygenase (tmo), catechol 1,2-dioxygenase, catechol 2,3-dioxygenase and toluene dioxygenase genes in dna extra ...200414624316
identification of novel eubacteria from spent mushroom compost (smc) waste by dna sequence typing: ecological considerations of disposal on agricultural land.a small study was undertaken to examine the microbiological characteristics of spent mushroom compost (smc), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. the primary aim of this study was to examine smc for the presence of faecal bacterial pathogens, including campylobacter spp., salmonella spp. and listeria monocytogenes. secondly it was desirable to quantify bacterial and fungal populations within smc, and ...200414672727
bacterial populations associated with the oxidation and reduction of arsenic in an unsaturated soil.microbial populations responsible for the oxidation and reduction of as were examined in unsaturated (aerobic) soil columns treated with 75 microm arsenite [as(iii)] or 250 microm arsenate [as(v)]. arsenite [as(iii)] was rapidly oxidized to as(v) via microbial activity, whereas no apparent reduction of as(v) was observed in the column experiments. eight aerobic heterotrophic bacteria with varying as redox phenotypes were isolated from the same columns. three isolates, identified as agrobacterium ...200414740724
[a new aerobic gram-positive bacterium with a unique ability to degrade ortho- and para-chlorinated biphenyls].strain b51 capable of degrading polychlorinated biphenyls (pcb) was isolated from soil contaminated with wastes from the chemical industry. based on its morphological and chemotaxonomic characteristics, the strain was identified as a microbacterium sp. experiments with washed cells showed that strain b51 is able to degrade ortho- and para-substituted mono-, di-, and trichlorinated biphenyls (mcb, dcb, and tcb, respectively). unlike the known pcb degraders, microbacterium sp. b51 is able to oxidi ...200314768541
coenzyme precursor-assisted cooperative overexpression of an active pyridoxine 4-oxidase from microbacterium luteolum.the overexpression system of the active pyridoxine 4-oxidase from microbacterium luteolum was developed. when chaperonin groel/es genes in plasmid pky206 were coexpressed, the pyridoxine 4-oxidase gene cloned in the vector ptrc99a was overexpressed in escherichia coli jm109 cultured in lb medium containing 50microm riboflavin, the precursor of coenzyme (fad) of the enzyme, under the cold stress at 23 degrees c. the crude extract from the cotransformant cells showed 88-fold higher specific activi ...200415003257
microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra.a xylanolytic and phosphate-solubilizing bacterium isolated from sawdust of ulmus nigra in salamanca was characterized by a polyphasic approach. the novel strain, designated xil02(t), was gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped and non-motile. phylogenetically and chemotaxonomically, it was related to members of the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium arborescens and microbacterium imperiale; howev ...200415023968
the crystal structure of a (-) gamma-lactamase from an aureobacterium species reveals a tetrahedral intermediate in the active site.the structure of the recombinant (-) gamma-lactamase from an aureobacterium species has been solved at 1.73a resolution in the cubic space group f23 with unit cell parameters a=b=c=240.6a. the trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. the structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. the associated density in the former structure has been interprete ...200415081810
improved production of bioactive glucosylmannosyl-glycerolipid by sponge-associated microbacterium species.the marine microbacterium species hp2 (dsm 12583), isolated from the sponge halichondria panicea, is able to produce a glucosylmannosyl-glycerolipid when grown on a complex medium with glucose. optimizing the carbon sources in shake flask experiments has shown that glycerol affords the highest specific glycoglycerolipid production. the product yield approached 300 mg/l or 25 mg/g biomass upon scaling up in a 40-l bioreactor volume. the native diglycosyl-glycerolipid ggl.2 strongly inhibited grow ...200415085410
loss of srf-3-encoded nucleotide sugar transporter activity in caenorhabditis elegans alters surface antigenicity and prevents bacterial adherence.during the establishment of a bacterial infection, the surface molecules of the host organism are of particular importance, since they mediate the first contact with the pathogen. in caenorhabditis elegans, mutations in the srf-3 locus confer resistance to infection by microbacterium nematophilum, and they also prevent biofilm formation by yersinia pseudotuberculosis, a close relative of the bubonic plague agent yersinia pestis. we cloned srf-3 and found that it encodes a multitransmembrane hydr ...200415123614
isolation and characterization of novel bacteria degrading polycyclic aromatic hydrocarbons from polluted greek soils.three bacterial strains, designated as wphe1, sphe1, and ophe1, were isolated from greek soils contaminated with polycyclic aromatic hydrocarbon (pah)-containing waste from the wood processing, steel, and oil refinery industries. wphe1, sphe1, and ophe1 were characterized and identified as species of pseudomonas, microbacterium, and paracoccus, respectively, based on gram staining, biochemical tests, phospholipid analysis, fame analysis, g+c content and 16s rrna gene sequence analysis. the resul ...200415133642
potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water.heterotrophic plate counts (hpcs) are commonly used to assess the general microbiological quality of drinking water. drinking water quality specifications worldwide recommend hpc limits from 100 to 500 cfu ml(-1). a number of recent studies revealed evidence that these bacteria may not be as harmless as generally accepted. it appears that immuno-compromised individuals are particularly at risk. this would include the very young and very old patients with diseases such as aids and patients on the ...200415145586
antitumor activity of levan polysaccharides from selected microorganisms.levans were isolated from the cultures of gluconoacetobacter xylinus (g-levan; mw = 40,000), microbacterium laevaniformans (m; mw = 710,000), rahnella aquatilis (r; mw = 380,000), and zymomonas mobilis (z; mw = 570,000). the levans were composed mainly of fructose residues when analyzed by tlc and hplc, and their main backbones were beta-(2,6)-linkages with beta-(2,1)-branches by gc-ms and nmr. in the in vitro antitumor activity test of the levans against eight different tumor cell lines, relati ...200415178007
characterization of bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, as determined by 16s rdna analysis.the bacterial diversity in pulque, a traditional mexican alcoholic fermented beverage, was studied in 16s rdna clone libraries from three pulque samples. sequenced clones identified as lactobacillus acidophilus, lactobacillus strain asf360, l. kefir, l. acetotolerans, l. hilgardii, l. plantarum, leuconostoc pseudomesenteroides, microbacterium arborescens, flavobacterium johnsoniae, acetobacter pomorium, gluconobacter oxydans, and hafnia alvei, were detected for the first time in pulque. identity ...200415183874
studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ...200415210998
effect of levan's branching structure on antitumor activity.levan produced from microbacterium laevaniformans kctc 9732 (m-levan) was isolated and treated with an inulinase to modify its branching structure. the chemical structures of levans were characterized, and the modified levans were applied on animal tumor cells to evaluate their antitumor activity. the gc-ms analysis indicated that beta-(2,1)-linked branches of m-levan were specifically hydrolyzed. as the ratio of applied inulinase to levan increased, the branching degree decreased proportionally ...200415225991
molecular cloning, expression, and properties of an unusual aldo-keto reductase family enzyme, pyridoxal 4-dehydrogenase, that catalyzes irreversible oxidation of pyridoxal.microbacterium luteolum yk-1 has pyridoxine degradation pathway i. we have cloned the structural gene for the second step enzyme, pyridoxal 4-dehydrogenase. the gene consists of 1,026-bp nucleotides and encodes 342 amino acids. the enzyme was overexpressed under cold shock conditions with a coexpression system and chaperonin groel/es. the recombinant enzyme showed the same properties as the m. luteolum enzyme. the primary sequence of the enzyme was 54% identical with that of d-threo-aldose 1-deh ...200415226311
characterization of a protease of a feather-degrading microbacterium characterize a new feather-degrading bacterium.200415242459
the erk map kinase cascade mediates tail swelling and a protective response to rectal infection in c. elegans.the nematode caenorhabditis elegans is proving to be an attractive model organism for investigating innate immune responses to infection. among the known pathogens of c. elegans is the bacterium microbacterium nematophilum, which adheres to the nematode rectum and postanal cuticle, inducing swelling of the underlying hypodermal tissue and causing mild constipation. we find that on infection by m. nematophilum, an extracellular signal-regulated kinase (erk) mitogen-activated protein (map) kinase ...200415268855
srf-3, a mutant of caenorhabditis elegans, resistant to bacterial infection and to biofilm binding, is deficient in glycoconjugates.srf-3 is a mutant of c. elegans that is resistant to infection by microbacterium nematophilum and to binding of the biofilm produced by yersinia pseudotuberculosis and yersinia pestis. recently, srf-3 was characterized as a nucleotide sugar transporter of the golgi apparatus occurring exclusively in hypodermal seam cells, pharyngeal cells, and spermatheca. based on the above observations, we hypothesized that srf-3 may have altered glyconjugates that may enable the mutant nematode to grow unaffe ...200415452127
two novel species of tetrodotoxin-producing bacteria isolated from toxic marine puffer fishes.out of eight dominant discrete bacterial colonies isolated and purified from the toxic marine puffer fishes collected in hong kong waters, two novel species of non-sporing, non-acid-fast and chemoorganotrophic bacteria capable of producing tetrodotoxin (ttx, a potent non-protein neurotoxin), as well as one previously reported and confirmed ttx-producing bacterium. they were identified as microbacterium arabinogalactanolyticum, serratia marcescens and vibrio alginolyticus, respectively, all of wh ...200415501290
crystallization and preliminary x-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway i of pyridoxine.pyridoxal 4-dehydrogenase (pldh; ec 1.1.107) is the second enzyme in the bacterial degradation pathway i of vitamin b(6), which catalyzes the oxidation of pyridoxal to 4-pyridoxolactone using nad(+). pldh from microbacterium luteolum, a dimeric protein with a subunit molecular weight of 38 kda, was crystallized at 277 k in a drop solution comprising 15%(w/v) polyethylene glycol 4000, 0.15 m sodium acetate, 7.5 mm n-octyl-beta-d-glucoside and 0.075 m tris-hcl ph 7.5 by the sitting-drop vapour-dif ...200415502327
bacterial diversity of a soil sample from schirmacher oasis, antarctica.the bacterial diversity of a soil sample collected in the vicinity of lake zub, schirmacher oasis, antarctica, was determined both by establishing pure colonies of culturable bacteria and by cloning the total 16s rdna of the soil and establishing the phylogeny of the clones. analysis of the 16s rrna gene clones indicated that the bacteria belonged to the classes alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria, gemmatimonas, bacteriodetes, actinobacteria, chloroflexi and chlamydia ...200415559969
bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations.heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ...200415574939
synthesis, solution behavior, thermal stability, and biological activity of an fe(iii) complex of an artificial siderophore with intramolecular hydrogen bonding networks.previously, an artificial siderophore complex, the iron(iii) complex with tris[2-[(n-acetyl-n-hydroxy)glycylamino]ethyl]amine (tage), was constructed in order to understand the effect of intramolecular hydrogen bonding interaction on the siderophore function, and its structural characterization in the solid state was reported (inorg. chem. 2001, 40, 190). in this paper, the solution behavior of the m(iii)-tage (m = fe, ga) system has been investigated using (1)h nmr, uv-vis, and fab mass spectro ...200415606204
[microbacterium oxydans, a symbiont of djungarian hamster, displaying probiotic properties].a resident microorganism (strain kho-17) was isolated from secretions of the specific glandular structures at the angles of mouth of djungarian hamster (phodopus campbelli). according to cultural, morphological, and physiological properties as well as to the phylogenetic analysis basing on the sequences of 16s rrna gene and analysis of the cell wall the strain was assigned to the species microbacterium oxydans. the bacterium isolated displayed probiotic properties when administered orally as a s ...200415609853
bacterial diversity in spent mushroom compost assessed by amplified rdna restriction analysis and sequencing of cultivated isolates.spent mushroom compost (smc) is the residual by-product of commercial agaricus spp. cultivation, and it is mainly composed of a thermally treated cereal straw/animal manure mixture colonized by the fungal biomass. research on the valorization of this material is mainly focusing on its use as soil conditioner and plant fertilizer. an investigation of the bacterial diversity in smc was performed using molecular techniques in order to reveal the origin of smc microflora and its potential effect on ...200415612633
production of dihydroxy c50-carotenoid by aureobacterium sp. ferm p-18698.the dihydroxy c(50)-carotenoid, decaprenoxanthin, was produced by aureobacterium sp. collected from sea water. the addition of d-psicose to the culture medium improved the growth of cells and the yield of the carotenoid. the (13)c-nmr spectrum of decaprenoxanthin, which has not previously been reported, was successfully measured.200415618644
effects of ph amendment on metal working fluid wastewater biological treatment using a defined bacterial consortium.the aim of this study was to determine whether ph amendment of a highly alkaline metal working fluid (mwf) wastewater would improve biological treatment in a bioreactor system following introduction of a bacterial inoculum (comprised of the following strains: agrobacterium radiobacter, comamonas testosteroni, methylobacterium mesophilicum, microbacterium esteraromaticum, and microbacterium saperdae). the ph values tested were 6, 7, 8, and 9. three replicate batch mode bioreactors inoculated with ...200515625673
microbacterium halotolerans sp. nov., isolated from a saline soil in the west of china.a gram-positive short rod isolated from a saline soil in china was characterized using a polyphasic approach. this actinobacterium grew over a wide salinity range [0-15 % nacl, 0-20 % kcl and 0-30 % (mgcl2).6h2o (w/v); optimum concentrations for growth were 5 % nacl, 5-10 % kcl, 10 % (mgcl2).6h2o]. the optimum growth temperature and ph were 28-30 degrees c and 7.0-8.0, respectively. chemotaxonomic features (peptidoglycan-type b2beta with glycolyl residues; major menaquinones mk-10 and mk-11; pre ...200515653854
expression and purification of a recombinant enantioselective amidase.microbacterium sp. aj115 metabolises a wide range of nitriles using the two-step nitrile hydratase/amidase pathway. in this study, the amidase gene of microbacterium sp. aj115 has been inserted into the pcal-n-ek expression vector and expressed in escherichia coli bl21(de3)plyss. the expressed protein is active in e. coli and expression of the amidase gene allows e. coli to grow on acetamide as sole carbon and/or nitrogen source. expression of active amidase in e. coli was temperature dependent ...200515721788
nitrile bioconversion by microbacterium imperiale cbs 498-74 resting cells in batch and ultrafiltration membrane bioreactors.the biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the nhase activity contained in resting cells of microbacterium imperiale cbs 498-74 was operated at 5, 10 and 20 degrees c in laboratory-scale batch and membrane bioreactors. the bioreactions were conducted in buffered medium (50 mm na(2)hpo(4)/nah(2)po(4), ph 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. the integral bioreactor performances were studied w ...200615739103
microbacterium oleivorans sp. nov. and microbacterium hydrocarbonoxydans sp. nov., novel crude-oil-degrading gram-positive bacteria.a taxonomic study of two crude-oil-degrading, gram-positive bacterial strains, designated bas69(t) and bnp48(t), revealed that they represent two novel microbacterium species. 16s rrna gene sequence similarity to their closest phylogenetic neighbours was 98.5 % for bas69(t) (microbacterium paraoxydans dsm 15019(t) and microbacterium saperdae dsm 20169(t)) and 99 % for bnp48(t) (microbacterium luteolum dsm 20143(t)). levels of dna-dna relatedness to the closest phylogenetic neighbours of both str ...200515774639
microbacterium natoriense sp. nov., a novel d-aminoacylase-producing bacterium isolated from soil in natori, japan.a rod-shaped, gram-positive bacterium, strain tnjl143-2(t), having n-acyl-d-amino acid amidohydrolase (d-aminoacylase) activity, was isolated from a soil sample from natori, japan. it was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 degrees c and a ph optimum for growth of 5-7. the 16s rrna gene sequence of the strain showed the highest similarities to members of the genus microbacterium, in particular, microbacterium aerolatum, ...200515774640
purification and characterization of hydantoin racemase from microbacterium liquefaciens aj 3912.a hydantoin racemase that catalyzed the racemization of 5-benzyl-hydantoin was detected in a cell-free extract of microbacterium liquefaciens aj 3912, a bacterial strain known to produce l-amino acids from their corresponding dl-5-substituted-hydantoins. this hydantoin racemase was purified 658-fold to electrophoretic homogeneity by serial chromatography. the n-terminal amino acid sequence of the enzyme showed homology with known hydantoin racemases from other microorganisms. the apparent molecu ...200515784981
characterisation of the nitrile hydratase gene clusters of rhodococcus erythropolis strains aj270 and aj300 and microbacterium sp. aj115 indicates horizontal gene transfer and reveals an insertion of is1166.the nitrile metabolising strains aj270, aj300 and aj115 were isolated from the same location. the strains have very similar nitrile metabolising profiles. sequencing of the 16s rrna gene indicates that strains aj270 and aj300 are novel strains of rhodococcus erythropolis while strain aj115 is a novel microbacterium strain very closely related to microbacterium oxydans and microbacterium liquefaciens. analysis of the structure of the nitrile hydratase/amidase gene clusters in the three strains in ...200515803388
reduction of cr(vi) by immobilized cells of desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776.hexavalent chromium, a carcinogen and mutagen, can be reduced to cr(iii) by desulfovibrio vulgaris ncimb 8303 and microbacterium sp. ncimb 13776. this study examined cr(vi) reduction by immobilized cells of the two strains in a common solution matrix using various entrapment matrices. chitosan and pva-borate beads did not retain integrity and supported low or no reduction of cr(vi) by the cells. a commercial preparation (lentikats) was stable but also did not support cr(vi) reduction. k-carragee ...200515818565
[auxin production by bacteria associated with orchid roots].bacteria associated with the roots of greenhouse tropical orchids were shown to produce indole-3-acetic acid (iaa) and to excrete it into the culture liquid. the presence and activity of iaa were demonstrated colorimetrically, by thin-layer chromatography, and by biotests. the associated bacteria varied in their ability to excrete indole compounds (1-28 microg/ml nutrient broth). addition of tryptophan to the growth medium enhanced phytohormone production. upon addition of 200 microg/ml tryptoph ...200515835779
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