TitleAbstractYear(sorted ascending)
culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp.a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ...200818670903
chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading.a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. complete cr(vi) reduction in aerobic cultures was achieved at a high concentration of 200 mg/l after incubation for only 65 hours. under anaerobic conditions up to 150 mg, cr(vi)/l was completely removed after incubating for 130 to 155 hours, still higher t ...200818701792
coevolution between attine ants and actinomycete bacteria: a reevaluation.we reassess the coevolution between actinomycete bacteria and fungus-gardening (attine) ants. actinomycete bacteria are of special interest because they are metabolic mutualists of diverse organisms (e.g., in nitrogen-fixation or antibiotic production) and because pseudonocardia actinomycetes are thought to serve disease-suppressing functions in attine gardens. phylogenetic information from culture-dependent and culture-independent microbial surveys reveals (1) close affinities between free-livi ...200818752608
chromium (vi) reduction in activated sludge bacteria exposed to high chromium loading: brits culture (south africa).a mixed-culture of bacteria collected from a wastewater treatment plant in brits, north-west province (south africa) biocatalytically reduced cr(vi) at much higher concentrations than previously observed in cultures isolated in north america. cr(vi) reduction rate up to 8 times higher than the rate in previous cultures was achieved by the brits culture under aerobic conditions. near complete cr(vi) reduction was observed in batches under initial concentrations up to 200mg cr(vi)/l after incubati ...200818760438
conversion of puerarin into its 7-o-glycoside derivatives by microbacterium oxydans (cgmcc 1788) to improve its water solubility and pharmacokinetic properties.microbacterium oxydans strain nj 6 isolated from soil samples converted puerarin into two novel compounds, puerarin-7-o-glucoside and puerarin-7-o-isomaltoside, via an unreported o-glycosylation of the phenolic hydroxyl group at the 7-position of puerarin. sucrose, maltotriose, and maltose could be used as glucosyl donors for glycosylation of puerarin, but uridine-diphosphate glucose, glucose, fructose, lactose, cyclodextrin, and starch could not. regardless of the position of b-ring in the (iso ...200818795283
identities of microbacterium spp. encountered in human clinical the present study, 50 strains of yellow-pigmented gram-positive rods that had been isolated from human clinical specimens and collected over a 5-year period were further characterized by phenotypic and molecular genetic methods. all 50 strains belonged to the genus microbacterium, and together they represented 18 different species. microbacterium oxydans (n = 11), m. paraoxydans (n = 9), and m. foliorum (n = 7) represented more than half of the strains included in the present study. the isola ...200818799696
infections caused by coryneform bacteria in pediatric oncology patients.invasive infections caused by coryneform bacteria are uncommon but have been reported with increasing frequency in recent decades, especially in immunocompromised persons. because pediatric experience is limited, we examined the epidemiology and clinical characteristics of these infections in children undergoing cancer therapy.200818174873
a novel recombinant system for functional expression of myonecrotic snake phospholipase a(2) in escherichia coli using a new fusion affinity tag.a novel recombinant expression system in escherichia coli was developed using conger eel galectin, namely, congerin ii, as an affinity tag. this system was applied for the functional expression of myotoxic lysine-49-phospholipase a(2) ([lys(49)]pla(2)), termed bpii and obtained from protobothrops flavoviridis (pf) venom. recombinant pf bpii fused with a congerin tag has been successfully expressed as a soluble fraction and showed better quantitative yield when folded correctly. the solubility of ...200818207418
microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain gsoil 259(t), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain gsoil 259(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens ifo 3750(t) (98.5 %) and microbacterium imperiale ifo 1261 ...200818218944
virulence of plant pathogenic bacteria attenuated by degradation of fatty acid cell-to-cell signaling factors.diffusible signal factor (dsf) is a fatty acid signal molecule involved in regulation of virulence in several xanthomonas species as well as xylella fastidiosa. in this study, we identified a variety of bacteria that could disrupt dsf-mediated induction of virulence factors in xanthomonas campestris pv. campestris. while many bacteria had the ability to degrade dsf, several bacterial strains belonging to genera bacillus, paenibacillus, microbacterium, staphylococcus, and pseudomonas were identif ...200818257682
assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches.microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ...200818280146
elucidation of 2-hydroxybiphenyl effect on dibenzothiophene desulfurization by microbacterium sp. strain zd-m2.the effect of 2-hydroxybiphenyl (2-hbp), the end product of dibenzothiophene (dbt) desulfurization via 4s pathway, on cell growth and desulfurization activity was investigated by microbacterium sp. the experimental results indicate that 2-hbp would inhibit the desulfurization activity. providing 2-hbp was added in the reaction media, the dbt degradation rate decreased along with the increase of 2-hbp addition. by contrast, cell growth would be promoted in the addition of 2-hbp at a low concentra ...200818296046
microbacterium hatanonis sp. nov., isolated as a contaminant of aerobic, rod-shaped, gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain fcc-01(t), originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. a 16s rrna gene sequence analysis revealed that the isolate belonged to the genus microbacterium and represented an evolutionary lineage that was distinct from recognized microbacterium species. cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars ...200818319473
characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients.over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ...200818335166
phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off shimokita peninsula."a meta-enzyme approach" is proposed as an ecological enzymatic method to explore the potential functions of microbial communities in extreme environments such as the deep marine subsurface. we evaluated a variety of extra-cellular enzyme activities of sediment slurries and isolates from a deep subseafloor sediment core. using the new deep-sea drilling vessel "chikyu", we obtained 365 m of core sediments that contained approximately 2% organic matter and considerable amounts of methane from offs ...200818368287
genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants.the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ...200818383223
chemostat selection of a bacterial community able to degrade s-triazinic compounds: continuous simazine biodegradation in a multi-stage packed bed biofilm reactor.using a successive transfer method on mineral salt medium containing simazine, a microbial community enriched with microorganisms able to grow on simazine was obtained. afterwards, using a continuous enrichment culture procedure, a bacterial community able to degrade simazine from an herbicide formulation was isolated from a chemostat. the continuous selector, fed with a mineral salt medium containing simazine and adjuvants present in the commercial herbicide formulation, was maintained in opera ...200818392868
the c. elegans glycosyltransferase bus-8 has two distinct and essential roles in epidermal morphogenesis.ventral enclosure in caenorhabditis elegans involves migration of epidermal cells over a neuroblast substrate and subsequent adhesion at the ventral midline. organisation of the neuroblast layer by ephrins and their receptors is essential for this migration. we show that bus-8, which encodes a predicted glycosyltransferase, is essential for embryonic enclosure and acts in or with ephrin signalling to mediate neuroblast organisation and to permit epidermal migration. bus-8 acts non-cell-autonomou ...200818395708
identification of cis-regulatory elements from the c. elegans t-box gene mab-9 reveals a novel role for mab-9 in hypodermal function.we have identified conserved non-coding elements (cnes) in the regulatory region of caenorhabditis elegans and caenorhabditis briggsae mab-9, a t-box gene known to be important for cell fate specification in the developing c. elegans hindgut. two adjacent cnes (a region 78 bp in length) are both necessary and sufficient to drive reporter gene expression in posterior hypodermal cells. the failure of a genomic mab-9::gfp construct lacking this region to express in posterior hypodermis correlates w ...200818402933
characterization of heavy metal-resistant endophytic bacteria from rape (brassica napus) roots and their potential in promoting the growth and lead accumulation of rape.two lead (pb)-resistant endophytic bacteria were isolated from rape roots grown in heavy metal-contaminated soils and characterized. a pot experiment was conducted for investigating the capability of the two isolates to promote the growth and pb uptake of rape from pb-amended soil. the two isolates were identified as pseudomonas fluorescens g10 and microbacterium sp. g16 based on the 16s rdna gene sequence analysis. strains g10 and g16 exhibited different multiple heavy metal and antibiotic resi ...200818490091
microbacterium aquimaris sp. nov., isolated from seawater.strains js54-2(t) and js63-1 were isolated from seawater at jeju, korea. cells of these strains were gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. the strains had chemotaxonomic markers that were consistent with their classification in the genus microbacterium, i.e. mk-11, mk-12 and mk-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, pept ...200818599704
microbacterium luticocti sp. nov., isolated from sewage sludge compost.strain sc-087b(t), isolated from sewage sludge compost during a study of bacterial diversity in composts, was characterized. the isolate was a gram-positive, short rod that was motile, catalase- and oxidase-negative and able to grow at 27-45 degrees c, ph 5.5-9.7 and in up to 10 % nacl. the peptidoglycan was of the b2beta type, containing the characteristic amino acids ornithine, homoserine and hydroxyglutamic acid. the muramic acid residues of the peptidoglycan were partially glycolylated. the ...200818599719
probing the critical residues for intramolecular fructosyl transfer reaction of a levan fructotransferase.levan fructotransferase (lftase) preferentially catalyzes the transfructosylation reaction in addition to levan hydrolysis, whereas other levan-degrading enzymes hydrolyze levan into a levan-oligosaccharide and fructose. based on sequence comparisons and enzymatic properties, the fructosyl transfer activity of lftase is proposed to have evolved from levanase. in order to probe the residues that are critical to the intramolecular fructosyl transfer reaction of the microbacterium sp. al-210 lftase ...200818600048
rheological characterization of levan polysaccharides from microbacterium laevaniformans.levan polysaccharides were produced from microbacterium laevaniformans and its rheological behaviors were characterized as a function of concentration and temperature. the intrinsic viscosity of the purified levan was determined to be 0.38dl/g at 25 degrees c which was relatively higher than that of levans from other microbial sources. the flow behaviors of the levan solutions were characterized by the increase in the shear stress, giving more increments in the shear rate. thus, the levan soluti ...200817931701
microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated this study we evaluated the capacity of a defined microbial consortium (five bacteria: mycobacterium fortuitum, bacillus cereus, microbacterium sp., gordonia polyisoprenivorans, microbacteriaceae bacterium, naphthalene-utilizing bacterium; and a fungus identified as fusarium oxysporum) isolated from a pahs contaminated landfarm site to degrade and mineralize different concentrations (0, 250, 500 and 1000 mg kg(-1)) of anthracene, phenanthrene and pyrene in soil. pahs degradation and mineraliz ...200817572084
sugar beet-associated bacterial and fungal communities show a high indigenous antagonistic potential against plant pathogens.the aim of this study was to analyze microbial communities in/on sugar beet with special focus on antagonists toward plant pathogens. for this purpose, the composition of microorganisms isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field-grown sugar beet plants was analyzed by a multiphasic approach at three different plant development stages at six locations in europe. the analysis of microbial communities by single strand conformation polymorphism (sscp) of 16s/18s ...200818060449
conversion of major ginsenoside rb1 to 20(s)-ginsenoside rg3 by microbacterium sp. gs514.ginseng saponin, the most important secondary metabolite in ginseng, has various pharmacological activities. many studies have been directed towards converting major ginsenosides to the more active minor ginsenoside, rg3. due to the difficulty in preparing ginsenoside rg3 enzymatically, the compound has been mainly produced by either acid treatment or heating. a microbial strain gs514 was isolated from soil around ginseng roots in a field and used for enzymatic preparation of the ginsenoside rg3 ...200817764709
[application of biological aerated filter in treating biomass wastewater and its microbial population characteristics].biomass wastewater contains plenty of cellulose, hemicellulose, starch, saccharide, organic acid and protein. it has high cod value and leads to environment pollution and resource waste. biomass wastewater was treated in this investigate using a biological aerated filter (baf) on laboratory scale, and the effect of treatment in different hydraulic retention time (hrt) had been studied. the results show that the optimum hrt is 10 h. the value of cod and tn in effluent is 74.90 mg/l and 1.21 mg/l ...200819186816
microbacterium kribbense sp. nov., isolated from soil.a gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated msl-04(t), was isolated from a soil sample collected from an agricultural field on bigeum island (republic of korea) and was subjected to polyphasic characterization to unravel its taxonomic position. the optimum growth temperature and ph were 28-30 degrees c and ph 7.2-7.4. chemotaxonomic characteristics such as b2beta-type peptidoglycan with glycolyl residues, mk-11 and mk-12 as the major menaquinones and a fatty ac ...200818984689
plasmid transformation and expression of the firefly luciferase in microbacterium testaceum type and endophytic colonizing field strains.microbacterium testaceum is a predominant endophytic bacterial species isolated from corn and sorghum in the midwestern united states. the development of genetic transfer systems for m. testaceum may enable its use for biocontrol and other applications. the type strain (ifo 12675) and field isolates (se017, se034, and ce648) were grown to mid-exponential phase, concentrated (1.0 x 1011 cfu x ml(-1)), electroporated (escherichia coli-clavibacter shuttle plasmid pdm302), and plated on tsa with 10 ...200818997853
the acyltransferase gene bus-1 exhibits conserved and specific expression in nematode rectal cells and reveals pathogen-induced cell swelling.susceptibility to the rectal pathogen microbacterium nematophilum provides a means of examining hindgut differentiation in c. elegans. mutants of bus-1 are resistant to infection with this pathogen. we show here that bus-1 encodes a predicted acyltransferase expressed in rectal epithelial cells (k, f, and u), suggesting its involvement in regional surface modification. bus-1 reporter genes were used to show spatial regulation by hindgut developmental control genes: egl-38, mab-9, and mab-23. a b ...200819035336
crystallization of the hydantoin transporter mhp1 from microbacterium liquefaciens.the integral membrane protein mhp1 from microbacterium liquefaciens transports hydantoins and belongs to the nucleobase:cation symporter 1 family. mhp1 was successfully purified and crystallized. initial crystals were obtained using the hanging-drop vapour-diffusion method but diffracted poorly. optimization of the crystallization conditions resulted in the generation of orthorhombic crystals (space group p2(1)2(1)2(1), unit-cell parameters a = 79.7, b = 101.1, c = 113.8 a). a complete data set ...200819052379
agrococcus versicolor sp. nov., an actinobacterium associated with the phyllosphere of potato plants.the taxonomic position of a group of actinobacterial strains isolated from the phyllosphere of potato plants was investigated by using a polyphasic approach. although the similarity values for their 16s rrna gene sequences suggested an intermediate position between microbacterium and agrococcus, the phylogenetic tree demonstrated a clear clustering of the representative strain, k 114/01(t), within the genus agrococcus. the presence of 2,4-diaminobutyric acid as the diagnostic diamino acid in the ...200819060068
microbacterium profundi sp. nov., isolated from deep-sea sediment of polymetallic nodule environments.a gram-positive, aerobic, neutrophilic and rod-shaped bacterium, strain shh49(t), was isolated from a deep-sea sediment sample collected from the east pacific polymetallic nodule region. the strain was able to grow within a temperature range of 4-35 degrees c and tolerated up to 7.5 % (w/v) nacl. strain shh49(t) was characterized chemotaxonomically by having mk-12 and mk-13 as predominant isoprenoid quinones, anteiso-c(15 : 0), iso-c(15 : 0), iso-c(16 : 0) and anteiso-c(17 : 0) as major fatty ac ...200819060085
growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp. during ripening in a cheese model medium.the growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp., some common but rarely mentioned cheese bacteria, were investigated in a cheese model deacidified by debaryomyces hansenii during the ripening process. our results show that these bacteria had distinct growth and cheese flavour production patterns during the ripening process. p. vulgaris had the greatest capacity to produce not only the widest variety but also the highest quantities of volatile ...200919083231
viable ultramicrocells in drinking examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems.200919040704
endoxanthanase, a novel beta-d-glucanase hydrolyzing backbone linkage of intact xanthan from newly isolated microbacterium sp. xt11.a novel endoxanthanase was produced and isolated from the culture of microbacterium sp. xt11 growing on xanthan. optimum ph and temperature for the enzyme activity were 6.0 and 35-40 degrees c, respectively. the endoxanthanase cleaves the backbone endo-beta-1,4-glucosidic linkage of the xanthan molecule, which is specific to the intact xanthan. however, the lyase-treated xanthan or carboxymethyl cellulose could not be cleaved by endoxanthanase.200919050833
novel n-acylhomoserine lactone-degrading bacteria isolated from the leaf surface of solanum tuberosum and their quorum-quenching properties.we isolated and identified ahl-degrading bacteria from the leaf surface of solanum tuberosum. the 16 isolates inactivated both short- and long-chain ahls. two of these isolates, identified as microbacterium testaceum, showed putative ahl-lactonase activity. these two strains interrupted quorum-sensing dependent bacterial infection by plant pathogen pectobacterium carotovorum subsp. carotovorum. m. testaceum strains, which were isolated in this study, might be useful in the biocontrol of plant di ...200919734660
characterization of the caenorhabditis elegans udp-galactopyranose mutase homolog glf-1 reveals an essential role for galactofuranose metabolism in nematode surface coat synthesis.galactofuranose (gal(f)), the furanoic form of d-galactose produced by udp-galactopyranose mutases (ugms), is present in surface glycans of some prokaryotes and lower eukaryotes. absence of the gal(f) biosynthetic pathway in vertebrates and its importance in several pathogens make ugms attractive drug targets. since the existence of gal(f) in nematodes has not been established, we investigated the role of the caenorhabditis elegans ugm homolog glf-1 in worm development. glf-1 mutants display sig ...200919751718
detection and hazard assessment of pathogenic microorganisms in medical wastes.this study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. medical wastes collected from 5 major hospitals in south korea were classified and stored at three different temperatures (-20, 6, and 30 degrees c). presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and b ...200919827491
[phylogenetic diversity of the culturable rare actinomycetes in marine sponge hymeniacidon perlevis by improved isolation media].based on the molecular diversity information, seven actinomycete-selective culture media and isolation conditions were modified to isolate and cultivate diverse rare actinomycetes from hymeniacidon perlevis.200919873748
[genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing].plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.200919873749
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
hydrolysis of fenamiphos and its toxic oxidation products by microbacterium sp. in pure culture and groundwater.a bacterium with an exceptional ability to hydrolyse fenamiphos and its toxic oxidation products fenamiphos sulfoxide and fenamiphos sulfone, all possessing poc bond was isolated from soil. based on 16s rrna gene determination, this bacterium was putatively identified as microbacterium esteraromaticum. the phenols (fenamiphos phenol, sulfoxide phenol and sulfone phenol) formed during bacterial hydrolysis resisted further degradation in mineral salts medium and sterile groundwater, but were trans ...200919195880
the c. elegans hox gene egl-5 is required for correct development of the hermaphrodite hindgut and for the response to rectal infection by microbacterium nematophilum.members of the hox gene family encode transcription factors that specify positional identity along the anterior-posterior axis of nearly all metazoans. one among the caenorhabditis elegans hox genes is egl-5. a deletion allele of egl-5 was isolated in a screen for animals which fail to develop swollen tails when exposed to the bacterial pathogen microbacterium nematophilum. we show that compromised rectal development, which occurs as a result of loss of egl-5 function, results in a failure of re ...200919232338
biological interactions to select biocontrol agents against toxigenic strains of aspergillus flavus and fusarium verticillioides from maize.biological control represent an alternative to the use of pesticides in crop protection. a key to progress in biological control to protect maize against fusarium verticillioides and aspergillus flavus maize pathogens are, to select in vitro, the best agent to be applied in the field. the aim of this study was to examine the antagonistic activity of bacterial and yeast isolates against f.verticillioides and a. flavus toxigenic strains. the first study showed the impact of bacillus amyloliquefaci ...200919247799
lateral transfer of genes for hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) degradation.recent studies demonstrated that degradation of the military explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by species of rhodococcus, gordonia, and williamsia is mediated by a novel cytochrome p450 with a fused flavodoxin reductase domain (xpla) in conjunction with a flavodoxin reductase (xplb). pulse field gel analysis was used to localize xpla to extrachromosomal elements in a rhodococcus sp. and distantly related microbacterium sp. strain ma1. comparison of rhodococcus rhodochrous 1 ...200919270122
impact of two bacterial biocontrol agents on bacterial and fungal culturable groups associated with the roots of field-grown assess the impact of bacillus amyloliquefaciens and microbacterium oleovorans on bacterial and fungal groups associated to the roots of field-grown maize.200919292823
sand aggregation by exopolysaccharide-producing microbacterium the rhizosphere, exopolymers are also known to be useful to improve the moisture-holding capacity. the ability of the isolates from coastal sand dunes to produce exopolymers was determined. among which the isolate, showing very high production of exopolysaccharide (eps), microbacterium arborescens--agsb, a facultative alkalophile was further studied for exopolymer production. the isolate a gram-positive non-spore forming, slender rod, catalase positive, oxidase negative, showed growth in 12% ...200919340484
microbacterium binotii sp. nov., isolated from human blood.two strains of non-spore-forming, rod-shaped, gram-positive bacteria, cip 101303(t) and cip 102116, were isolated from human blood in 1976 and 1977, respectively. these strains had chemotaxonomic markers that were consistent with classification in the genus microbacterium, i.e. mk-10, mk-11 and mk-12 as the major menaquinones, predominant iso- and anteiso-branched cellular fatty acids, galactose, mannose and rhamnose as the cell-wall sugars and ornithine as the diamino acid in the cell-wall pept ...200919406785
assessment of the microbial diversity at the surface of livarot cheese using culture-dependent and independent approaches.the microbial diversity of the surface of a commercial red-smear cheese, livarot cheese, sold on the retail market was studied using culture-dependent and independent approaches. forty yeasts and 40 bacteria from the cheese surface were collected, dereplicated using single-strand conformation polymorphism (sscp) analysis and identified using rrna gene sequencing for the culture-dependent approach. the culture-independent approach involved cloning and sequencing of the 16s rrna gene and sscp anal ...200919481828
characterization of microbacterium sp. f10a and its role in polycyclic aromatic hydrocarbon removal in low-temperature soil.a polycyclic aromatic hydrocarbon degrading bacterium isolated from oil-polluted soil was identified as microbacterium sp. f10a based on 16s rdna gene sequence analysis. plant growth promoting characteristics of the strain, degradation rate of phenanthrene and pyrene, and cell surface hydrophobicity characteristics of the strain were further characterized. the strain was also evaluated for promoting the growth of wheat and phenanthrene and pyrene removal from soil artificially contaminated with ...200919483781
microbacterium insulae sp. nov., isolated from soil.a gram-positive, non-motile, rod- or coccoid-shaped microbacterium-like bacterium, designated strain ds-66(t), was isolated from soil of dokdo, korea, and its exact taxonomic position was investigated by using a polyphasic approach. strain ds-66(t) grew optimally at 30 degrees c and ph 6.5-7.0 in the presence of 0.5-1.0 % (w/v) nacl. phylogenetic analysis based on 16s rrna gene sequences showed that strain ds-66(t) belonged to the genus microbacterium. strain ds-66(t) had a peptidoglycan type ba ...200919542140
microbacterium invictum sp. nov., isolated from homemade compost.strain dc-200t was isolated from homemade compost produced from kitchen refuse and characterized using a polyphasic approach. the isolate was a gram-positive motile short rod, facultatively aerobic, catalase-positive and oxidase-negative, and was able to grow at 10-37 degrees c, ph 6.0-9.5 and with up to 5% of nacl. the peptidoglycan was of the type b1 alpha and the muramic acid residues were glycolylated. the major fatty acids were anteiso-c15:0 and anteiso-c17:0. the predominant respiratory me ...200919567581
occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland.the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ...200919572487
stereospecific degradation of phenylsuccinate by actinomycetes.racemic phenylsuccinate was stereospecifically degraded by the actinomycetes ps9 and ps17 isolated from soil obtained from okinawa island, japan. strain ps9, identified as a citricoccus sp., preferentially degraded the r-form, while strain ps17, identified as a microbacterium sp., preferentially degraded the s-form of phenylsuccinate. analysis of the culture broths of these species with phenylsuccinate as the sole carbon source revealed that benzoic acid was produced as a metabolic intermediate. ...200919580991
mutation of asparagine 76 in the center of glutamine transporter snat3 modulates substrate-induced conductances and na+ binding.the glutamine transporter slc38a3 (snat3) plays an important role in the release of glutamine from brain astrocytes and the uptake of glutamine into hepatocytes. it is related to the vesicular gaba (gamma-aminobutyric acid) transporter and the slc36 family of proton-amino acid cotransporters. the transporter carries out electroneutral na+-glutamine cotransport-h+ antiport. in addition, substrate-induced uncoupled cation currents are observed. mutation of asparagine 76 to glutamine or histidine i ...200919596860
isolation and characterization of biosurfactant-producing alcanivorax strains: hydrocarbon accession strategies and alkane hydroxylase gene analysis.biosurfactant-producing bacteria belonging to the genera alcanivorax, cobetia and halomonas were isolated from marine sediments with a history of hydrocarbon exposure (aristizábal and gravina peninsulas, argentina). two alcanivorax isolates were found to form naturally occurring consortia with strains closely related to pseudomonas putida and microbacterium esteraromaticum. alkane hydroxylase gene analysis in these two alcanivorax strains resulted in the identification of two novel alkb genes, s ...200918983915
vertical distribution and anaerobic biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments in hong kong, south china.the vertical distribution of polycyclic aromatic hydrocarbons (pahs) at different sediment depths, namely 0-2 cm, 2-4 cm, 4-6 cm, 6-10 cm, 10-15 cm and 15-20 cm, in one of the most contaminated mangrove swamps, ma wan, hong kong was investigated. it was the first time to study the intrinsic potential of deep sediment to biodegrade pahs under anaerobic conditions and the abundance of electron acceptors in sediment for anaerobic degradation. results showed that the total pahs concentrations (summa ...200919683792
isolation of identical nitrilase genes from multiple bacterial strains and real-time pcr detection of the genes from soils provides evidence of horizontal gene transfer.bacterial enzymes capable of nitrile hydrolysis have significant industrial potential. microbacterium sp. aj115, rhodococcus erythropolis aj270 and aj300 were isolated from the same location in england and harbour identical nitrile hydratase/amidase gene clusters. strain aj270 has been well studied due to its nitrile hydratase and amidase activity. r. erythropolis itcbp was isolated from denmark and carries a very similar nitrile hydratase/amidase gene cluster. in this study, an identical nitril ...200919730817
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
identification and characterization of bacteria isolated under selective pressure of volatile organic compounds.several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ...200920112870
characterization of phosphobacteria isolated from eutrophic aquatic ecosystems.phosphobacteria are able to enhance phosphorus availability in soil and improve crop yields. to develop such biofertilizers, 14 predominant phosphobacteria were isolated from eutrophic aquatic ecosystems. molecular identification and phylogenetic analysis revealed three groups among the nine isolates of inorganic phosphate-solubilizing bacteria (ipsb): ipsb1 and ipsb2 belonged to the actinobacteria and flavobacteria, respectively, and the other seven belonged to the gamma-proteobacteria. among f ...200920170019
screening and distributing features of bacteria with hydantoinase and carbamoylase.hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ...200917498938
treatment of bromoamine acid wastewater using combined process of micro-electrolysis and biological aerobic filter.the wastewater originated from the production of bromoamine acid was treated in a sequential system of micro-electrolysis (me) and biological aerobic filter (baf). decolorization and cod(cr) removal rate of the proposed system was investigated with full consideration of the influence of two major controlling factors such as organic loading rate (olr) and hydraulic retention time (hrt). the removal rate of cod(cr) was 81.2% and that of chrominance could be up to 96.6% at an olr of 0.56 kg m(-3)d( ...200918621476
enhanced removal of dimethyl sulfide from a synthetic waste gas stream using a bioreactor inoculated with microbacterium sp. ntut26 and pseudomonas putida.the removal of dimethyl sulfide (dms) from industrial gas streams has received a high priority due to its very low odorous threshold value and relatively low biodegradability compared to other reduced sulfur compounds. a variety of bacteria that utilize dms as a carbon/energy source have been studied and the degradation pathway elucidated. however, to date, there have been few reports on the industrial application of such bacteria inoculated into a bioreactor for dms treatment. an additional pro ...200918815822
application of compochip microarray to investigate the bacterial communities of different composts.a microarray spotted with 369 different 16s rrna gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. the microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). labeled target dna was prepared by polymerase chain reaction amplification of 16s rrna genes using a cy5-labeled universa ...200918818861
characterisation of aerobically grown non-spore-forming bacteria from paper mill pulps containing recycled fibres.a total of 179 non-spore-forming bacteria aerobically growing on nutrient agar, plate count agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. after phenotypical screening the isolates were characterised by automated ribotyping and partial sequencing of the 16s rrna gene. they could be divided into seven taxonomical classes representing 63 taxa (species): actinobacteria (11 species), ba ...200918820960
medium optimization for the production of cyclic adenosine 3',5'-monophosphate by microbacterium sp. no. 205 using response surface methodology.response surface methodology was employed to optimize medium composition for the production of cyclic adenosine 3',5'-monophosphate (camp) with microbacterium sp. no. 205. a fractional factorial design (2(11-7)) was applied to evaluate the effects of different components in the medium. k(2)hpo(4), mgso(4) and naf were found to significantly influence on the camp production. the steepest ascent method was used to access the optimal region of the medium composition. the concentrations of the three ...200918778935
isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the southwest indian ocean ridge.microorganisms play an important role in the geobiocycling of arsenic element. however, little is known about the bacteria involved in this process in oceanic environments. in this report, arsenite-resistant bacteria were detected in deep-sea sediments on the southwest indian ridge. from arsenite enriched cultures, 54 isolates were obtained, which showed varied tolerance to arsenite of 2-80 mm. phylogenetic analysis based on 16s rrna showed that they mainly belonged to proteobacteria and actinob ...200918841325
aiim, a novel class of n-acylhomoserine lactonase from the leaf-associated bacterium microbacterium testaceum.n-acylhomoserine lactones (ahls) are used as quorum-sensing signal molecules by many gram-negative bacteria. we have reported that microbacterium testaceum stlb037, which was isolated from the leaf surface of potato, has ahl-degrading activity. in this study, we cloned the aiim gene from the genomic library of stlb037, which has ahl-degrading activity and shows high homology with the alpha/beta hydrolase fold family from actinobacteria. purified aiim as a maltose binding fusion protein showed hi ...201020173075
level of chemical and microbiological contaminations in chili bo (paste).the objective of this study was to determine the level of preservatives and microbiological loads in various brands of commercially available chili bo (paste). fifteen different brands of chili bo obtained from the local market and hypermarkets were analyzed for ph, moisture and benzoic acid content, microbiological loads (aerobic, anaerobic, aerobic spores, and fungi), and thermophilic microorganisms. results showed that both moisture content and ph vary among samples. the concentrations of ben ...201020202342
construction and analysis of a bacterial community exhibiting strong chitinolytic activity.a stable bacterial community expressing strong chitinolytic activity was constructed by mixing and cultivating chitinolytic bacteria collected from different natural sources. the dna fragment pattern, after pcr-denaturing gradient gel electrophoresis (dgge) targeting 16s rrna genes using total dna prepared from whole cells, indicated that the community was composed of four dominant bacterial species. all four species were isolated on agar medium, and one strain, say3, was deduced to be a novel s ...201020208340
population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis.surface contamination of smear cheese by listeria spp. is of major concern for the industry. complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties in situ were investigated by tem ...201020222967
symbiotic relationship between microbacterium sp. sk0812 and candida tropicalis sk090404.a bacterium growing inside yeast cytoplasm was observed by light microscope without staining. the bacterium was separately stained from yeast cell by a fluorescent dye, 4',6-diamidino-2-phenylindole (dapi). the bacterium actively moved inside yeast cytoplasm and propagated in company with the yeast growth. the bacterium was separated from the yeast cytoplasm by selective disruption of yeast cells and the yeast without the intracellular bacterium (ywob) was obtained by selective inactivation of b ...201020127466
gene cloning and characterization of dihydrolipoamide dehydrogenase from microbacterium luteolum: a useful enzymatic regeneration system of nad+ from nadh.dihydrolipoamide dehydrogenase (lpd), a useful biocatalyst for regenerating nad(+), was purified from microbacterium luteolum jcm 9174, and the gene encoding lpd was cloned from the genomic dna. the gene contained an opening reading frame consisting of 1395 nucleotides encoding 465 amino acid residues with a predicted molecular weight of 49912.1 da, which displayed 36-78% homology to known lpds. moreover, the fad- and nad(+)-binding sites and the two catalytic residues in the lpds were conserved ...201020159566
arsenic-resistant bacteria associated with roots of the wild cirsium arvense (l.) plant from an arsenic polluted soil, and screening of potential plant growth-promoting characteristics.a rhizobacterial community, associated with the roots of wild thistle cirsium arvense (l.) growing in an arsenic polluted soil, was studied by fluorescence in situ hybridization (fish) analysis in conjunction with cultivation-based methods. in the bulk, rhizosphere, and rhizoplane fractions of the soil, the qualitative picture obtained by fish analysis of the main phylogenetic bacterial groups was similar and was predominantly comprised of alphaproteobacteria, betaproteobacteria, and gammaproteo ...201020303688
microbacterium radiodurans sp. nov., a uv radiation-resistant bacterium isolated from soil.strain gimn 1.002(t), a uv radiation-tolerant bacterium, was isolated from the upper sand layers of the gobi desert, xinjiang, china and characterized in order to determine its taxonomic position. cells were gram-reaction-positive, heterotrophic, strictly aerobic, short rods. 16s rrna gene sequence analysis revealed that strain gimn 1.002(t) belonged to the genus microbacterium and was closely related to microbacterium arborescens dsm 20754(t) (98.8 % 16s rrna gene sequence similarity) and micro ...201020061506
microbacterium ginsengiterrae sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.strain dcy37(t) was isolated from a soil sample of a ginseng field in the republic of korea and characterized in order to determine its taxonomic position. cells were gram-staining-positive, heterotrophic, strictly aerobic, non-motile short rods. 16s rrna gene sequence analysis revealed that strain dcy37(t) belongs to the genus microbacterium. according to 16s rrna gene sequence analysis, it is closely related to microbacterium aerolatum dsm 14217(t) (98.8 %), microbacterium hydrocarbonoxydans d ...201020081025
polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent.biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ...201020093025
selection of candidate probionts by two different screening strategies from atlantic cod (gadus morhua l.) larvae.two primary selection criteria were used to collect a pool of nearly 500 candidate probiotic bacteria from atlantic cod (gadus morhua l.) larvae, i.e. the dominant intestinal bacterial flora and isolates with antagonistic activity against vibrio anguillarum. bacteria were isolated from cod larvae from five rearing groups with variable rearing technologies. the bacteria were brought to pure culture and characterized phenotypically. based on properties such as uniqueness, dominance and fermentativ ...201020097491
microbacterium azadirachtae sp. nov., a plant-growth-promoting actinobacterium isolated from the rhizoplane of neem seedlings.microbacterium strain ai-s262(t) was isolated from the rhizoplane of neem seedlings in the botanical garden of tamilnadu agricultural university, coimbatore, india, and subjected to phenotypic, chemotaxonomic and genetic characterization. cells of this strain were gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. strain ai-s262(t) contained mk-12 and mk-13 as the main respiratory quinones, anteiso-c(15 : 0), anteiso-c(17 : 0) ...201019734284
isolation of some pathogenic bacteria from the great spruce bark beetle, dendroctonus micans and its specific predator, rhizophagus grandis.some bacteria were isolated from dendroctonus micans and its specific predator, rhizophagus grandis. six bacteria from d. micans were identified as bacillus pumilus, enterobacter intermedius, citrobacter freundii, cellulomonas flavigena, microbacterium liquefaciens and enterobacter amnigenus, three bacteria from r. grandis as klebsiella pneumoniae, pantoea agglomerans and serratia grimesii, on the basis of fatty acid methyl ester analysis and carbon utilization profile by using microbial identif ...201020336502
the caenorhabditis elegans bus-2 mutant reveals a new class of o-glycans affecting bacterial resistance.microbacterium nematophilum causes a deleterious infection of the c. elegans hindgut initiated by adhesion to rectal and anal cuticle. c. elegans bus-2 mutants, which are resistant to m. nematophilum and also to the formation of surface biofilms by yersinia sp., carry genetic lesions in a putative glycosyltransferase containing conserved domains of core-1 beta1,3-galactosyltransferases. bus-2 is predicted to act in the synthesis of core-1 type o-glycans. this observation implies that the infecti ...201020385555
biochemical characterization of gram-positive and gram-negative plant-associated bacteria with micro-raman spectroscopy.raman spectra of gram-positive and gram-negative plant bacteria have been measured with micro-raman spectrometers equipped with 785 and 514.5 nm lasers. the gram-positive bacteria microbacterium testaceum, paenibacillus validus, and clavibacter michiganensis subsp. michiganensis have strong carotenoid bands in the regions 1155-1157 cm(-1) and 1516-1522 cm(-1) that differentiate them from other tested gram-negative bacteria. in the raman spectrum of gram-positive bacteria bacillus megaterium exci ...201020412629
molecular basis of alternating access membrane transport by the sodium-hydantoin transporter mhp1.the structure of the sodium-benzylhydantoin transport protein mhp1 from microbacterium liquefaciens comprises a five-helix inverted repeat, which is widespread among secondary transporters. here, we report the crystal structure of an inward-facing conformation of mhp1 at 3.8 angstroms resolution, complementing its previously described structures in outward-facing and occluded states. from analyses of the three structures and molecular dynamics simulations, we propose a mechanism for the transpor ...201020413494
evaluation of microbial dynamics during the ripening of a traditional taiwanese naturally fermented ham.isolation and identification of the autochthonous starter from a naturally fermented meat allows control of the fermentation process and promises microbiological safety for this specialty. thus the purpose of this study was to identify the lactic acid bacteria and coagulase-negative cocci present in a traditional taiwanese naturally fermented ham (tnfh) and to study the microbial dynamics at different ripening stages; the approach was a combination of conventional microbiological cultivation, po ...201020417394
isolation and characterization of fenamiphos degrading bacteria.the biological factors responsible for the microbial breakdown of the organophosphorus nematicide fenamiphos were investigated. microorganisms responsible for the enhanced degradation of fenamiphos were isolated from soil that had a long application history of this nematicide. bacteria proved to be the most important group of microbes responsible for the fenamiphos biodegradation process. seventeen bacterial isolates utilized the pure active ingredient fenamiphos as a carbon source. sixteen isol ...201020464454
selected microbial glycolipids: production, modification and characterization.this chapter deals with two types of biosurfactants that are not in the spotlight of general research: glycoglycerolipids and oligosaccharide lipids. the main focus is on glycolglycerolipids from marine bacteria like microbacterium spec. dsm 12583, micrococcus luteus (hel 12/2) and bacillus pumilus strain aas3 and on oligosaccharide lipids from tsukamurella spec. dsm 44370 and nocardia corynebacteroides sm1. general and special structures, microbial producers, production conditions and chemo-enz ...201020545283
enantioselective resolution of γ-lactam by a whole cell of microbacterium hydrocarbonoxydans (l29-9) immobilized in polymer of pva-alginate-boric acid.using immobilized cells of a novel strain of microbacterium hydrocarbonoxydans l29-9 in polymers of polyvinyl alcohol (pva)-alginate-boric acid, enantioselective resolution of racemic γ-lactam to produce (-)γ-lactam was successfully carried out. a 6:1 ratio of pva:sodium alginate not only prevented agglomeration of the matrix but also produced beads with high gel strength. the optimum biotransformation conditions were 1 g/l substrate, ph 7.0, reaction temperature of 30 °c, and reaction time of 3 ...201020563863
identities of epilithic hydrocarbon-utilizing diazotrophic bacteria from the arabian gulf coasts, and their potential for oil bioremediation without nitrogen supplementation.gravel particles from four sites along the arabian gulf coast in autumn, winter, and spring were naturally colonized with microbial consortia containing between 7 and 400 × 10(2) cm(-2) of cultivable oil-utilizing bacteria. the 16s rrna gene sequences of 70 representatives of oil-utilizing bacteria revealed that they were predominantly affiliated with the gammaproteobacteria and the actinobacteria. the gammaproteobacteria comprised among others, the genera pseudomonas, pseudoalteromonas, shewane ...201020567813
[analysis of microbial diversity of nitrifying bacteria by terminal restriction fragment length polymorphism].we analyzed the microbial diversity and quantity of nitrifying bacteria in the enrichment reactor by terminal restriction fragment length polymorphism (t_rflp), a cultured-independent molecular technique. the result indicated that nitrobacteria enriched the best, and the diversity index decreased 62.80% compared with the initial data. nitrobacteria were predominant in the reactor. meanwhile, we studied the microbial diversity before and after adding nitrobacteria into shrimp ponds, and analyzed ...201020575436
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
size-resolved culturable airborne bacteria sampled in rice field, sanitary landfill, and waste incineration sites.size-resolved bacterial concentrations in atmospheric aerosols sampled by using a six stage viable impactor at rice field, sanitary landfill, and waste incinerator sites were determined. culture-based and polymerase chain reaction (pcr) methods were used to identify the airborne bacteria. the culturable bacteria concentration in total suspended particles (tsp) was found to be the highest (848 colony forming unit (cfu)/m(3)) at the sanitary landfill sampling site, while the rice field sampling si ...201020623053
impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against fusarium verticillioides.bacillus amyloliquefaciens and microbacterium oleovorans reduced the fusarium verticillioides count and significantly decreased fumonisin b(1) and b(2) levels in maize grains. the aim of this study was to determine the effect of water stress tolerance and heat shock survival upon cells of the biocontrol agents b. amyloliquefaciens and m. oleovorans. the a(w) of solid and liquid media and tryptic soy medium was modified to 0.99, 0.98, 0.97 and 0.96 by addition of ionic solute nacl and non-ionic s ...201020624459
microbacterium arthrosphaerae sp. nov., isolated from the faeces of the pill millipede arthrosphaera magna attems.a gram-reaction-positive, rod-shaped bacterium, designated strain cc-vm-y(t), was isolated from the faeces of the pill millipede arthrosphaera magna attems from india and was subsequently studied to determine its taxonomic position. based on16s rrna gene sequence similarities, the isolate clearly grouped with members of the genus microbacterium. on the basis of pairwise comparisons of the 16s rrna gene sequences, strain cc-vm-y(t) was most closely related to microbacterium insulae ds-66(t) (98 % ...201020656817
culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot.microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ...201020657618
long-term hg pollution-induced structural shifts of bacterial community in the terrestrial isopod (porcellio scaber) previous studies we detected lower species richness and lower hg sensitivity of the bacteria present in egested guts of porcellio scaber (crustacea, isopoda) from chronically hg polluted than from unpolluted environment. basis for such results were further investigated by sequencing of 16s rrna genes of mercury-resistant (hgr) isolates and clone libraries. we observed up to 385 times higher numbers of hgr bacteria in guts of animals from polluted than from unpolluted environment. the majority ...201020724045
isolation and characterization of hexavalent chromium-reducing rhizospheric bacteria from a wetland.scirpus americanus pers. occurs naturally in "san germán," a pond that serves as a receptor of industrial wastewater in guanajuato, méxico. this plant accumulates metals mainly in the root: concentrations (mg/kg) of cr, as, cd and se were 970, 49, 41, and 85 respectively. analysis of rhizosphere samples indicated bacterial population of 10(8) cfu g(-1) in media with 0.2 mm cr(vi) and 10 mm sodium gluconate. thirteen isolates were obtained and phylogenetic analyses (16s rrna) indicated they corre ...201020734910
microbacterium soli sp. nov., an alpha-glucosidase-producing bacterium isolated from soil of a ginseng field.five gram-type-positive, aerobic, rod-shaped, non-motile strains of microbacterium (dcy 17(t), ms1, ms2, ms3 and ms4) were isolated from soil from a ginseng field in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, these strains were shown to be related to microbacterium esteraromaticum dsm 8609(t) (96.1 %), m. xylanilyticum dsm 16914(t) (96.0 %), m. aquimaris js54-2(t) (95.6 %), m. insulae ds-66(t) (95.5 %), m. ketosireducens ifo 14548(t) (95.5 %) and m. arabinogalactano ...201019654351
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