Publications

the growtn of microbacterium thermosphactum on beef.		1979	44291
isoprenoid quinones in the classification of coryneform and related bacteria.	menaquinones were the only isoprenoid quinones found in 85 of the 95 coryneform bacteria examined. dihydromenaquinones having nine isoprene units were the main components isolated from corynebacterium bovis, from other glutamic acid-producing strains, and from arthrobacter globiformis and related species. dihydromenaquinones with eight isoprene units were found in brevibacterium linens, the remaining corynebacterium species and strains probably belonging to the genus rhodococcus. tetrahydromenaq ...	1979	107269
energetics of microbacterium thermosphactum in glucose-limited continuous culture.	microbacterium thermosphactum was grown at 25 degrees c in glucose-limited continuous culture under aerobic (greater than 120 microm oxygen) and anaerobic (less than 0.2 microm oxygen) conditions. the end products of the anaerobic metabolism of glucose were identified as l-lactate and ethanol. together these compounds accounted for between 85 and 90% of the glucose utilized over the full range of growth rates studied. in addition, 4% of the glucose utilized was incorporated into cellular materia ...	1979	114113
the microbiology of polony.	a survey of 25 polony samples of different brands available to the consumer in bloemfontein showed that significant numbers of various micro-organisms were present. aerobic micro-organisms, psychrophiles, micrococci, pseudomonads, microbacterium thermosphactum, lactobacilli, yeasts and moulds were enumerated by the plate count technique. the mean aerobic plate count was 15,850/g and counts ranged between less than 100/g and greater than 1 million/g. selective plate counts indicated that psychrop ...	1978	366151
[destruction of microbacterium lacticum, escherichia coli and staphylococcus aureus in milk by spray-drying. i. selective count of surviving bacteria].	in this paper a method which allows the measure of microbial death rate during spray-drying by means of a streptomycin-resistant mutant that can be grown on a streptomycin-containing agar is described. plate counts of microbacterium lacticum, escherichia coli, and staphylococcus aureus recovered from skim milk powders were done on plate count agar in the presence and absence of streptomycin and on various selective media. the powders were produced from evaporated milk previously inoculated with ...	1977	406026
[destruction of microbacterium lacticum, escherichia coli and staphylococcus aureus in milk by sprayedrying. ii. effect of drying conditions].	bacterial death during spray drying of skim milk is essentially related to the outlet temperature of the spray drier and the type of bacteria. under industrial spray drying conditions, survival rates of microbacterium lacticum, staphylococcus aureus and escherichia coli were 50, 2, and 0.002%, respectively. these rates may vary by a 10(4) factor for outlet temperatures between 65 and 105 degrees c. no simple mathematical equation could be derived to describe the relation between bacterial death ...	1977	406027
[preservation of meats in a controlled atmosphere].	commercializing meat sets problems more particularly connected with its organoleptic and microbiological qualities. as these qualities, resulting from biochemical and microbial effects, develop during the storage, the conditioning must precisely help their controlling. after examining their factors, the author analyses the processes of conditioning which he classifies in three categories according to the techniques used and the desired finality (need to store at -1 degree c + 3 degrees c). 1. co ...	1978	707932
effect of lactobacilli and carbon dioxide on the growth of microbacterium thermosphactum on fresh beef.	studies with articifially inoculated fresh beef showed that lactobacilli markedlyrestrict the growth of microbacterium thermosphactum when the beef is vacuum-packaged. high concentrations of carbon dioxide (up to 75%) had little effect on the growthof m. thermosphactum under either aerobic or anaerobic conditions. tests were madewith samples of lean beef inoculated with pure cultures of lactobacilli and (or) m. thermosphactum and stored unpackaged in air, in air enriched with carbon dioxide, in ...	1975	804984
a numerical taxonomic study of coryneform and related bacteria.	two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ...	1975	805825
a numerical taxonomic survey of listeria and related bacteria.	a numerical taxonomic study was performed on named strains of listeria, erysipelothrix, microbacterium thermosphactum, lactobacillus, streptococus, propionibacterium, kurthia and some possibly related bacteria using i43 unit characters covering a wide range of properties. the strains fell into six main clusters: (a) listeria; (b) microbacterium thermosphactum, lactobacillus, streptococcus; (c) gemella, erysipelothrix; (d) kurthia and mainly aerobic corynebacteria; (e) propionibacterium, staphylo ...	1977	856940
[survival of epiphytic microorganisms following sublimation dehydration and storage].	epiphytic microorganisms belonging to the genera pseudomonas, agrobacterium, achromobacter, flavobacterium, mycobacterium, mycococcus, nocardia, corynebacterium, arthrobacter, bacillus, and microbacterium were dehydrated by sublimation employing two suspension media, and were stored at 3-5 degrees c during 1-2.5 years. survival of epiphytic microorganisms upon dehydration by sublimation and storage was found to depend on their taxonomy (genus, species, strain) and the composition of the suspensi ...	1976	1004279
identification of the cultivable bacteria in dental plaque from the beagle dog.	fifteen different genera of microorganisms were isolated from the 27-month-old plaque of two beagle dogs. they were as follows: pseudomonas, proteus, neisseria, escherichia, staphylococcus, streptococcus, lactobacillus, nocardia, actinomyces, corynebacterium, micrococcus, microbacterium, brevibacterium, arthrobacter, and kurthia. the dog plaque was characterized by a low number of streptococci and the presence of several noncarbohydrate-fermenting organisms.	1976	1069758
isolation of mutants with improved production of camp from microbacterium sp. no. 205 (atcc 21376).	mutants were isolated from microbacterium sp. no. 205 (atcc 21376) producing 13-30 mm cyclic adenosine 3',5'-monophosphate (camp) by salvage biosynthesis, through sequential improvements of the bacterium for the purpose of improving camp production. the mutants produced 50-75 mm camp on 100 mm inosine 5'-monophosphate as a precursor. mutants resistant to the inhibition of growth by camp at high concentrations were isolated; the resistance was one of four characteristics effective for improved pr ...	1990	1369318
culture media for non-sporulating gram-positive food spoilage bacteria.	the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ...	1992	1486021
aspei, a novel eam11051 isoschizomer from aureobacterium species recognizing 5'-gacnnn/nngtc-3'.		1992	1641345
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi.	linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ...	1991	1657086
the specificity of bacterial siderophore receptors probed by bioassays.	the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...	1991	1663779
microbial metabolism of quinoline and related compounds. xi. degradation of quinoline-4-carboxylic acid by microbacterium sp. h2, agrobacterium sp. 1b and pimelobacter simplex 4b and 5b.	from soil enrichment cultures four strains, using quinoline-4-carboxylic acid as sole source of energy and carbon, have been isolated. according to their physiological properties these bacteria have been identified as microbacterium sp. designated h2, as agrobacterium sp. designated 1b and pimelobacter simplex designated 4b and 5b. metabolites of the degradation pathway of quinoline-4-carboxylic acid have been isolated and identified. with pimelobacter simplex 4b and 5b 2-oxo-1,2-dihydroquinolin ...	1991	1793516
transfructosylation of rebaudioside a (a sweet glycoside of stevia leaves) with microbacterium beta-fructofuranosidase.	it was found that a beta-fructofuranosidase produced by microbacterium sp. h-1 has potent trans-beta-fructofuranosylation activity from sucrose (donor). by means of this enzyme system, rebaudioside a (ra), the second major sweet steviol glycoside of the leaves of stevia rebaudiana, was subjected to transfructosylation, affording a mono-beta-fructofuranosylated product (ra-f) in a high yield. the structure of ra-f was elucidated as beta-d-fructofuranosyl-(2----6)-beta-d-glucopyranosyl ester of st ...	1991	1797424
mami, a novel class-ii restriction endonuclease from microbacterium ammoniaphilum recognizing 5'-gatnn decreases nnatc-3'.	a new site-specific class-ii restriction endonuclease, mami, has been discovered in the nonsporulating gram+ microbacterium ammoniaphilum. mami recognition sequence and cleavage positions were deduced using experimental and computer-assisted mapping and sequencing approaches. mami cleavage specificity corresponds to: [formula: see text] the novel 43-kd enzyme recognizes a palindromic hexanucleotide interrupted by four ambiguous nucleotides. mami cleavage positions are located in the center of th ...	1990	2401411
degradation of quinoline-4-carboxylic acid by microbacterium sp.	from soil enrichment culture of quinoline-4-carboxylic acid-degrading bacterium was isolated. the organism was identified as microbacterium sp. mutants were induced with n-methyl-n'-nitro-n-nitrosoguanidine. one mutant accumulated successively two metabolites which were identified as 2-oxo-1,2-dihydro-quinoline-4-carboxylic acid and 8-hydroxy-2-oxo-2h-1-benzopyran-4-carboxylic acid.	1989	2498156
evidence for a new pathway in the bacterial degradation of 4-fluorobenzoate.	six bacterial strains able to use 4-fluorobenzoic acid as their sole source of carbon and energy were isolated by selective enrichment from various water and soil samples from the stuttgart area. according to their responses in biochemical and morphological tests, the organisms were assigned to the genera alcaligenes, pseudomonas, and aureobacterium. to elucidate the degradation pathway of 4-fluorobenzoate, metabolic intermediates were identified. five gram-negative isolates degraded this substr ...	1989	2604392
regulation of the glucose phosphotransferase system in brochothrix thermosphacta by membrane energization.	uptake of 2-deoxyglucose, alpha-methylglucopyranoside, and glucose into intact cells of brochothrix thermosphacta (formerly microbacterium thermosphactum, atcc 11509) was stimulated by kcn or cccp. the glucose analogs were recovered almost totally as the sugar phosphates. membrane vesicles were isolated from protoplasts and shown to be right side out by freeze fracturing and by using atpase as a marker for the cytoplasmic membrane surface. uptake of glucose into vesicles was dependent on the pre ...	1985	2995314
phylogenetic analysis of the coryneform bacteria by 5s rrna sequences.	nucleotide sequences of 5s rrnas from 11 coryneform bacteria were determined. these were the type strains of corynebacterium glutamicum, corynebacterium xerosis, brevibacterium linens, arthrobacter globiformis, cellulomonas biazotea, aureobacterium testaceum, curtobacterium citreum, pimelobacter simplex, and caseobacter polymorphus and representative strains of "corynebacterium aquaticum" and corynebacterium xerosis. a phylogenetic tree constructed from the sequences of these bacteria and publis ...	1987	3106318
the thoracic surgical spectrum of acquired immune deficiency syndrome.	the acquired immune deficiency syndrome has presented a complex and, as yet, unsolvable spectrum of pulmonary disease characterized by bizarre infections, pneumothoraces, respiratory distress, and death. thirty-eight patients underwent 49 surgical procedures during 42 months. ages of the patients ranged from 24 to 56 years. surgical procedures included tracheostomy, closure of air leaks, mediastinoscopy, lobectomy, open lung biopsy, and esophagogastrectomy. hospital mortality was 10 of 38 (24%); ...	1986	3784593
microbacterium lacticum, troublemaker in blood fractions.		1974	4218564
cytochrome as a guide to classifying bacteria: taxonomy of microbacterium thermosphactum.		1968	4301060
production of coenzyme a by sarcina lutea.	to develop an efficient method for the production of coenzyme a (coa), optimal conditions for its formation from pantothenic acid, cysteine, and adenine were studied. a number of microorganisms were screened for production of coa. strains belonging to the genera sarcina, bacillus, microbacterium, micrococcus, and serratia accumulated coa. among these, sarcina lutea was selected as the best organism, and the culture conditions for the production of coa were investigated with this organism. under ...	1974	4844273
numerical taxonomy of certain coryneform bacteria.	da silva, g. a. nigel (iowa state university, ames), and john g. holt. numerical taxonomy of certain coryneform bacteria. j. bacteriol. 90:921-927. 1965-an electronic computer was used to sort 32 strains of coryneforms into groups with the tree-sort program. the similarity values obtained in this procedure were then used to construct a dendrogram depicting the phenetic resemblance among the taxa. the results indicated that all the phytopathogens studied were sufficiently distinct from the type s ...	1965	4954898
[the murein types of the genus microbacterium].		1970	5469569
a study of the lipid composition of microbacterium thermosphactum as a guide to its taxonomy.		1970	5491625
carbon dioxide fixation and the synthesis of aspartate by microbacterium thermosphactum.		1970	5495738
the lipid composition of microbacterium lacticum.		1968	5639272
effect of gamma irradiation on the microflora of freshwater fish. 3. spoilage patterns and extension of refrigerated storage life of yellow perch fillets irradiated to 0.1 and 0.2 megarad.	maximal shelf life was determined and microbial flora were compared for irradiated (0.1 and 0.2 mrad) and nonirradiated yellow perch fillets stored at 1 c. shelf life was estimated by organoleptic determinations. microbiological studies included determination of the effects of irradiation on the total aerobic microbial population, lag phase, and rate of growth. genera of organisms isolated from fillets through the course of microbial spoilage were identified, and the proteolytic activity of the ...	1968	5645411
[the amino acid sequence of the murein of microbacterium lacticum].		1968	5650421
some morphological and physiological properties of microbacterium thermosphactum.		1968	5702042
a selective medium for the enumeration of microbacterium thermosphactum in meat and meat products.		1966	5980913
some observations on the taxonomy of the genus microbacterium. i. cultural and physiological reactions and heat resistance.		1966	5980923
some observations on the taxonomy of the genus microbacterium. ii. cell wall analysis, gel electrophoresis and serology.		1966	5980924
numerical taxonomy of gram-positive and catalase-positive rods isolated from frozen vegetables.	one hundred isolates from peas, beans, and corn were compared with cultures of corynebacterium, microbacterium, and arthrobacter by use of numerical taxonomic procedures. six groups (clusters), representing 75% of the isolates, resembled corynebacteriaceae. there was some doubt regarding the genera represented because the groups were not closely related to many of the known strains. the relationship of the different groups to each other, as well as a number of their properties, is presented.	1967	6031430
the amino acid sequence of a glycine-containing cell wall precursor of microbacterium lacticum.		1967	6033749
identification of threo-3-hydroxyglutamic acid in the cell wall of microbacterium lacticum.		1967	6072398
13c nuclear magnetic resonance studies of the biosynthesis by microbacterium ammoniaphilum of l-glutamate selectively enriched with carbon-13.	13c nmr of isotopically enriched metabolites has been used to study the metabolism of microbacterium ammoniaphilum, a bacterium which excretes large quantities of l-glutamic acid into the medium. biosynthesis from 90% [1-13c]glucose results in relatively high specificity of the label, with [2,4-13c2]glutamate as the major product. the predominant biosynthetic pathway for synthesis of glutamate from glucose was determined to be the embden meyerhof glycolytic pathway followed by p-enolpyruvate car ...	1982	6120165
protoplast transformation of glutamate-producing bacteria with plasmid dna.	a method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid dna was established. protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. the concentration of penicillin during growth affected the efficiency of formation, regeneration, and polyethylene glycol-induced dna uptake of protoplasts. regeneration of protoplasts was accomplished on a hypertonic agar medium containing so ...	1984	6145700
nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa.	thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ...	1982	6183393
a phylogenetic analysis of the family dermatophilaceae.	the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ...	1983	6195302
identification key for coryneform bacteria derived by numerical taxonomic studies.	six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ...	1983	6413644
threo-beta-hydroxyornithine: a natural constituent of the peptidoglycan of corynebacterium species co 112.	peptidoglycan of corynebacterium species co 112 (dsm 20606) exhibits an unknown amino acid. the amino acid was isolated from cell wall hydrolysates and identified as threo-beta-hydroxyornithine. this amino acid is found in the interpeptide bridge of the peptidoglycan of corynebacterium sp. co 112. the primary structure of this peptidoglycan is rather similar to that of microbacterium liquefaciens. the only difference is the replacement of ornithine by threo-beta-hydroxyornithine. the mode of lin ...	1983	6615130
energetics of growth of microbacterium thermosphactum at low temperatures.	microbacterium thermosphactum was grown at 5 degrees c in glucose-limited continuous cultures. the end products of glucose metabolism were l-lactate and ethanol, and these compounds accounted for 86--92% of the glucose utilized. with input glucose concentrations less than 3 mv, yglumax was found to be 40--43, yatpmax 20--21 and ms 0.1--0.2. these values are almost identical to those found previously for cultures at 25 degrees c and show that this psychrotroph grows with a very high energetic eff ...	1980	6782998
numerical classification of some rhodococci, corynebacteria and related organisms.	nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ...	1982	6811696
a preferential isolation procedure for asporogenous gram-positive bacteria.	a preferential isolation procedure was devised for asporogenous (asp), gram-positive (gp), aerobic or facultative anaerobic bacteria which included the genera arthrobacter, corynebacterium, brevibacterium, microbacterium, mycobacterium, and micrococcus (asp-gp bacteria). an antibiotics-mixture agar which contained 5 to 10 micrograms per ml of colistin, 10 to 20 micrograms per ml of nalidixic acid and 30 micrograms per ml of cycloheximide was used in the isolation. using this technique 47 asp-gp ...	1982	7096199
bacterial growth in ground beef prepared from electrically stimulated and nonstimulated muscles.	ground beef samples prepared from electrically stimulated and nonstimulated biceps femoris and infraspinatus muscles were inoculated with lactobacillus sp., pseudomonas sp., acinetobacter sp., or a mixture of lactobacillus spp., pseudomonas spp., acinetobacter spp., moraxella sp., microbacterium thermosphactum, and erwinia herbicola. there were no significant differences in growth of various bacteria in ground beef made from electrically stimulated and nonstimulated muscles.	1981	7195194
phylogenetic analysis of the genus microbacterium based on 16s rrna gene sequences.	16s rrna gene (rdna) studies of the six species of the genus microbacterium, m. lacticum, m. laevaniformans, m. dextranolyticum, m. imperiale, m. arborescens and m. aurum, were performed and the primary structures were compared with those of 29 representative actinobacteria and related organisms. phylogenetic analysis indicated that six species of the genus microbacterium and representative four species of the genus aureobacterium appear to be phylogenetically coherent as was suggested by rainey ...	1994	7528152
primary identification of microbacterium spp. encountered in clinical specimens as cdc coryneform group a-4 and a-5 bacteria.	over nearly two decades, 13 yellow- or orange-pigmented, fermentative gram-positive rods belonging to the genus microbacterium were encountered in clinical specimens. all 13 strains, 10 of which came from blood cultures, were initially identified as cdc coryneform group a-4 and a-5 bacteria according to the scheme of hollis and weaver for the identification of gram-positive rods. the clinical isolates were compared with the type strains of the six species constituting the genus microbacterium as ...	1995	7699039
formation of beta-fructosyl compounds of pyridoxine in growing culture of aspergillus niger.	two pyridoxine compounds were found to be formed in a culture filtrate of aspergillus niger and a. sydowi, when grown in a medium containing sucrose and pyridoxine. each of the two compounds i and ii was obtained as a white powdered preparation by preparative paper chromatography, gel filtration on toyopearl hw-40s and sephadex g-10 columns, deae-cellulose column chromatography, and lyophilization. compounds i and ii were identified as 5'-o-(beta-d-fructofuranosyl)-pyridoxine and 5'-o-[beta-d-fr ...	1993	7763873
primary identification of aureobacterium spp. isolated from clinical specimens as "corynebacterium aquaticum".	over a 6-year period 11 yellow-pigmented gram-positive rods (gprs) with an oxidative carbohydrate metabolism were isolated from clinical specimens or were received as reference cultures and tentatively identified as "corynebacterium aquaticum" according to the guide of hollis and weaver for the differentiation of gprs (d. g. hollis and r. e. weaver, gram-positive organisms: a guide to identification, 1981). because these isolates seemed to be rather heterogeneous, comparative analyses with the t ...	1994	7852557
an outbreak of ochrobactrum anthropi bacteraemia in five organ transplant patients.	nosocomial bacteraemia caused by ochrobactrum anthropi occurred over a 1-month period in five organ transplant recipients, four of whom were in the same renal and pancreatic transplant unit. bacteraemia occurred with cyclosporin a, azathioprine and steroids, and with a rabbit anti-thymocyte globulin (ratg) during the induction phase. ratg appeared to be the only common factor among the five cases. over the period described, 71.4% of all patients receiving ratg developed o. anthropi bacteraemia. ...	1994	7916361
in situ probing of gram-positive bacteria with high dna g + c content using 23s rrna-targeted oligonucleotides.	23s-rrna-targeted oligonucleotide probes were designed for the phylogenetic group 'gram-positive bacteria with high g + c content of dna' (gpbhgc). a sequence idiosyncrasy in two adjacent base pairs in the stem of helix 69 in domain iv of the 23s rrna is present in all hitherto analysed strains of gpbhgc. an oligonucleotide probe targeted to this region hybridized only with strains of gpbhgc and was successfully used for in situ monitoring of these cells in activated sludge. another unique featu ...	1994	8000548
suprageneric classification of peptidoglycan group b actinomycetes by nucleotide sequencing of 5s ribosomal rna.	5s ribosomal rna sequences were determined for thirteen actinomycetes mainly representatives with the rare group b type peptidoglycan. the primary and secondary structure of the resultant sequences were of the type characteristic of gram-positive bacteria with dna rich in guanine plus cytosine. the sequencing and associated chemotaxonomic data provide compelling grounds for classifying actinomycetes with a group b type peptidoglycan in a single family. the family microbacteriaceae fam. nov. is p ...	1993	8085792
proposal of six new species in the genus aureobacterium and transfer of flavobacterium esteraromaticum omelianski to the genus aureobacterium as aureobacterium esteraromaticum comb. nov.	twelve strains placed in the genera flavobacterium, pseudomonas, and aureobacterium, including soil isolates, were characterized taxonomically. on the basis of morphological, physiological, and chemotaxonomic data, as well as dna-dna hybridization data, we propose that 11 of these strains should be classified in the genus aureobacterium as new combinations or new species, as follows: aureobacterium esteraromaticum comb. nov. (type strain, ifo 3751 [= atcc 8091]), aureobacterium arabinogalactanol ...	1993	8347513
polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge.	eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera curtobacterium and aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. all isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mu mg-1. polyphosphate:amp phosphotransferase could only be detected in one isolate. three isolates showed a polyphosphate kinase activity als ...	1993	8386121
chemotaxonomy and molecular taxonomy of some coryneform clinical isolates.	six reference strains of the genus brevibacterium as well as fifteen clinical isolates tentatively assigned to the genus using conventional biochemical methods, were the subject of chemotaxonomic and dna similarity studies. five of these clinical isolates were assigned either to the genera aureobacterium, mycobacterium, gordona or to rhodococcus on the basis of their dna mol% g+c, mycolic acid, amino-acid, sugar and menaquinone contents. among the ten remaining strains, six were not brevibacteri ...	1993	8518510
[hypersensitivity pneumonitis caused by a home humidifier].	a 33-year-old man was admitted complaining of a fever, dyspnea, and a dry cough almost every night since december of 1992. he had been using an ultrasonic humidifier at home. the chest ct scan and roentgenogram showed bilateral reticulonodular shadows. after admission, the symptoms resolved spontaneously. these findings were suggestive of hypersensitivity pneumonitis. after analysis of fluid obtained by bronchoalveolar lavage and of a specimen obtained by transbronchial biopsy, "humidifier lung" ...	1995	8538084
cloning and characterization of the mami restriction-modification system from microbacterium ammoniaphilum in escherichia coli.	the genes encoding a class-iin restriction-modification (r-m) system (mami, sequence specificity [symbol: see text] from microbacterium ammoniaphilum have been cloned in escherichia coli. the vector used for cloning was plasmid puc18 modified by the inclusion of three mami recognition sites. recombinant clones containing the mamim gene in its genomic context became fully methylated in vivo and remained completely resistant against digestion with the r.mami restriction endonuclease (enase). deter ...	1996	8654988
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probes	the structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...	1996	8688004
case of fatal systemic infection with an aureobacterium sp.: identification of isolate by 16s rrna gene analysis.	the case of a 75-year-old man who succumbed to a disseminated infection most likely caused by a species of the genus aureobacterium is reported. identification of the isolate was achieved by comparative 16s rrna gene analysis. aureobacteria are commonly found in the environment. however, only recently have they been recognized as a cause of infections including septicemia and soft tissue infections. to our knowledge, this is the first documentation of a fatal infection caused by an aureobacteriu ...	1996	8735113
purification and characterization of a prolidase from aureobacterium esteraromaticum.	an edta-insensitive prolidase (proline dipeptidase, ec 3.4.13.9) was isolated from a cell-free extract of aureobacterium esteraromaticum ifo 3752. the enzyme was purified almost to homogeneity using acetone precipitation, hydrophobic chromatography, ion-exchange chromatography, and gel-permeation chromatography. the enzyme has a molecular weight of about 440,000 by gel permeation chromatography, and about 40,000 by sds polyacrylamide gel electrophoresis. the isoelectric point was 4.6. the enzyme ...	1996	8782407
bioavailability of selenium accumulated by selenite-reducing bacteria.	the bioavailability of selenium (se) was determined in bacterial strains that reduce selenite to red elemental se (seo). a laboratory strain of bacillus subtilis and a bacterial rod isolated from soil in the vicinity of the kesterson reservoir, san joaquin valley, ca, (microbacterium arborescens) were cultured in the presence of 1 mm sodium selenite (na2seo3). after harvest, the washed, lyophilized b. subtilis and m. arborescens samples contained 2.62 and 4.23% total se, respectively, which was ...	1996	8811279
vancomycin-resistant aureobacterium species cellulitis and bacteremia in a patient with acute myelogenous leukemia.	a 39-year-old male with acute myelogenous leukemia and concomitant porphyria cutanea tarda was admitted to the hospital for consolidation chemotherapy of his leukemia. during his hospitalization, he developed cellulitis of the left hand and persistent bacteremia with a yellow-pigmented, nonfermenting coryneform bacterium that was identified as aureobacterium sp. the portal of entry for the aureobacterium infection was probably through the skin lesions due to porphyria cutanea tarda. the infectio ...	1996	8818896
an identification scheme for rapidly and aerobically growing gram-positive rods.	an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ...	1996	8837385
the application of serological techniques to the taxonomy of arthrobacter and related organisms.	antisera were raised against rods of 17 named arthrobacter and aureobacterium strains. antigenic relationships between these strains, other soil bacteria and new arthrobacter isolates from several soils were studied, using agglutination, immunodiffusion, immunofluorescence and elisa techniques. many of the named arthrobacter species had common antigens, and there were also common antigens amongst named arthrobacter strains and many fresh arthrobacter isolates. agglutination, elisa and immunofluo ...	1996	8868431
degradation of prochloraz and 2,4,6-trichlorophenol by environmental bacterial strains.	eight bacterial isolates from enrichment with 2,4,6-trichlorophenol (tcp) as sole carbon source were tested for their potential to degrade prochloraz. none of them could grow on prochloraz. strain c964, identified as aureobacterium sp., effectively reduced the fungitoxic activity of prochloraz in a bioassay and degradation was confirmed by hplc. two other isolates, strain c611 and c961, using tcp as a carbon source, belong to the beta subclass of the proteobacteria and presumely degrade tcp via ...	1996	8920198
clinical microbiology of coryneform bacteria.	coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ...	1997	8993861
purification and characterization of dipeptidyl aminopeptidase from aureobacterium sp. wo26.	we isolated a bacterial strain with an enzyme which releases dipeptide from gly-arg-p-nitroanilide. the bacterium was tentatively identified as aureobacterium sp. the enzyme, named audap, was purified and characterized. it was homogenous by sds-page and ief, and had a molecular mass of 90,000 da by sds-page and 88,000 da by gel filtration, so it may be a monomer. the isoelectric point was 3.8 and the optimum ph was 10.0. the purified enzyme hydrolyzed gly-arg-pna, a model substrate for dap i, an ...	1997	9028041
endophthalmitis due to microbacterium species: case report and review of microbacterium infections.	microbacterium species (formerly cdc [centers for disease control and prevention] coryneform group a-4 and a-5 bacteria) are widely distributed in the environment and rarely cause infections in humans. we present a case of endophthalmitis due to microbacterium species that occurred after accidental trauma and review the literature on microbacterium infections. if the infected tissue or medical device is removed and antimicrobial therapy (preferably with beta-lactams or glycopeptides) is institut ...	1997	9145748
activity of rifabutin, clarithromycin, ethambutol, sparfloxacin and amikacin, alone and in combination, against mycobacterium avium complex in human macrophages.	disseminated infection with microbacterium avium complex (mac) in patients with aids is currently treated with a combination of antimycobacterial agents in order to prevent the selection of resistant mutant strains. although clinical and microbiological responses can generally be achieved within a few weeks, relapses are common and require modification of the combination regimen or identification of effective alternate therapies. in this study we investigated the activities of rifabutin 0.5 mg/l ...	1996	9182107
[microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].	microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ...	1996	9190234
[microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].	microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ...	1996	9190236
multicenter evaluation of the updated and extended api (rapid) coryne database 2.0.	in a multicenter study, 407 strains of coryneform bacteria were tested with the updated and extended api (rapid) coryne system with database 2.0 (biomérieux, la-balme-les-grottes, france) in order to evaluate the system's capability of identifying these bacteria. the design of the system was exactly the same as for the previous api (rapid) coryne strip with database 1.0, i.e., the 20 biochemical reactions covered were identical, but database 2.0 included both more taxa and additional differentia ...	1997	9399506
aureobacterium resistens sp. nov., exhibiting vancomycin resistance and teicoplanin susceptibility.	two similar strains of a coryneform bacterium were isolated from human clinical material. both strains were resistant to vancomycin but susceptible to teicoplanin. detailed biochemical, chemotaxonomical, and molecular genetic investigations revealed that both isolates were members of a hitherto undescribed species of genus aureobacterium. the name aureobacterium resistens sp. nov. is proposed for the new bacterium and the type strain is ccug 38312.	1998	9453161
thermostable phenylalanine dehydrogenase from a mesophilic microbacterium sp. strain dm 86-1	bacteria that produced nad+-dependent phenylalanine dehydrogenase (ec 1.4.1.20) were selected among l-methionine utilizers isolated from soil. a bacterial strain showing phenylalanine dehydrogenase activity was chosen and classified in the genus microbacterium. phenylalanine dehydrogenase was purified from the crude extract of microbacterium sp. strain dm 86-1 (tpu 3592) to homogeneity as judged by sds-polyacrylamide disc gel electrophoresis. the enzyme has an isoelectric point of 5.8 and a rela ...	1998	9477256
[rapid diagnosis of tuberculosis using the amplification method].	amplification methods identify microbacterium tuberculosis on the basis of genus- or species-specific sequence of bases in nucleic acids which they replete exponentially. the objective of the work was comparison of results of biological samples by the method gen-probe mycobacterium tuberculosis direct test (mtd) using amplification of ribosomal rna and bk by microscopy and cultivation, assessment of standard indicators of their efficiency of the method and analysis of diverging results.	1997	9511260
psychrotrophic bacteria isolated from a constantly warm tropical environment.	psychrotrophic bacteria are known to occur in temperate, constantly cold, and artificially cooled environments. this is the first report of their occurrence in a constantly warm (ca. 24 degrees-35 degrees c) tropical environment. soil samples taken from two sites along the southeastern coastal zone of jamaica yielded growth of psychotrophic bacteria after 3-4 weeks of enrichment culture in 1/30 strength tryptic soy broth, 20 mg l-1 cycloheximide at 2 degrees c. growth of individual isolates at 2 ...	1998	9516542
biodegradation of metal-edta complexes by an enriched microbial population.	a mixed culture utilizing edta as the sole carbon source was isolated from a mixed inoculum of water from the river mersey (united kingdom) and sludge from an industrial effluent treatment plant. fourteen component organisms were isolated from the culture, including representatives of the genera methylobacterium, variovorax, enterobacter, aureobacterium, and bacillus. the mixed culture biodegraded metal-edta complexes slowly; the biodegradability was in the order fe > cu > co > ni > cd. by incor ...	1998	9546167
serum antibodies reacting with subgingival species in refractory periodontitis subjects.	the purpose of this investigation was to compare the levels of serum igg antibody to 85 subgingival species in 32 refractory periodontitis, 56 successfully treated, and 33 periodontally healthy subjects. refractory subjects showed mean full mouth attachment loss and/or >3 sites showing attachment loss >2.5 mm within 1 year after 2 treatment modalities, scaling and root planing and surgery plus systemically administered tetracycline. successfully-treated subjects showed mean attachment level gain ...	1998	9696261
microbial communities of printing paper machines.	the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ...	1998	9717292
union of the genera microbacterium orla-jensen and aureobacterium collins et al. in a redefined genus microbacterium.	the 16s rrna gene sequences of 19 strains, 11 strains representing validated aureobacterium or microbacterium species and eight strains of non-valid species or isolates, were determined. these sequences were aligned with the sequences of other validated aureobacterium and microbacterium species and related actinobacteria. a comparative sequence analysis of 43 strains revealed that the species of the genera aureobacterium and microbacterium form a monophyletic association in which species of both ...	1998	9734028
proposal of six new species in the genus microbacterium and transfer of flavobacterium marinotypicum zobell and upham to the genus microbacterium as microbacterium maritypicum comb. nov.	reference strains, including two mis-named organisms, 'chromobacterium chocolatum' and flavobacterium marinotypicum, isolates from soil and clinical specimens, all previously recognized as aureobacterium or microbacterium, were characterized taxonomically. on the basis of morphological, physiological and chemotaxonomic characteristics, as well as dna-dna hybridization data, six new species and one new combination are proposed in the genus microbacterium: microbacterium ketosireducens sp. nov. (t ...	1998	9734054
etherolytic cleavage of 4-(2,4-dichlorophenoxy)butyric acid and 4-(4-chloro-2-methylphenoxy)butyric acid by species of rhodococcus and aureobacterium isolated from an alkaline environment.	bacterial strains were isolated from the concrete rubble of a demolished herbicide production plant. the predominant feature of these strains was the etherolytic cleavage of 4-(2,4-dichlorophenoxy)butyric acid (dcpb)1) and 4-(4-chloro-2-methylphenoxy)butyric acid (mcpb) while liberating 2,4-dichlorophenol (dcp) and 4-chloro-2-methylphenol (mcp) respectively. some of the isolates were identified by 16s rdna sequence analysis and shown to belong to the genera aureobacterium sp. (strain k2-17) and ...	1998	9867509
cloning of a novel prolidase gene from aureobacterium esteraromaticum.	the prolidase gene from aureobacterium esteraromaticum was cloned and expressed in escherichia coli. the cloned enzyme had the same enzymatic properties as the wild-type enzyme. kinetic analysis of the enzyme indicated that the best substrate was pro-hyp, which was not hydrolyzed by other prolidases. interestingly, there was no homology between the deduced amino acid sequence of a. esteraromaticum prolidase and those of the other sources such as human e. coli and lactobacillus. however, homology ...	1999	9989239
application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria.	strains belonging to the gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (tgge). this method allows the sequence-specific separation of amplified fragments of 16s rrna genes. a total of 115 reference strains representing the majority of the species of the genera aeromicrobium, agromyces, arthrobacter, aureobacterium, cellulomonas, curtobacterium, nocardioides and terrabacter were characterized. depending on the genus investigated, th ...	1999	10028252
reclassification of brevibacterium oxydans (chatelain and second 1966) as microbacterium oxydans comb. nov.	phylogenetic and chemotaxonomic analyses indicate that brevibacterium oxydans is closely related to species of the genus microbacterium, namely microbacterium liquefaciens, microbacterium luteolum and microbacterium saperdae. dna-dna reassociation values of less than 60% between brevibacterium oxydans and these three microbacterium species support the distinctness of this misclassified brevibacterium species, which is reclassified as microbacterium oxydans comb. nov.	1999	10028259
effect of elevated oxygen and carbon dioxide on the surface growth of vegetable-associated micro-organisms.	the impact of a novel type of modified atmosphere (ma), referred to as high o2-ma, on micro-organisms associated with the spoilage of minimally-processed vegetables was studied. pure cultures of pseudomonas fluorescens, enterobacter agglomerans, aureobacterium strain 27, candida guilliermondii, c. sake, salmonella typhimurium, salm. enteritidis, escherichia coli, listeria monocytogenes, leuconostoc mesenteroides var. mesenteroides, lactobacillus plantarum and lactococcus lactis were cultured on ...	1999	10196748
actinomycetes in karstic caves of northern spain (altamira and tito bustillo).	a variety of isolation procedures were carried out to study the involvement of bacteria in the colonisation and biodeterioration of spanish caves with paleolithic rock art (altamira and tito bustillo). the applied techniques mainly aimed to isolate heterotrophic bacteria such as streptomycetes, nocardioform and coryneform actinomycetes, and other gram-positive and gram-negative bacteria. the results demonstrated that actinomycetes were the most abundant gram-positive bacteria in the caves. actin ...	1999	10353805
analysis of the bacterial surface ripening flora of german and french smeared cheeses with respect to their anti-listerial potential.	the anti-listerial potential of 19 different french smeared cheese bacterial consortia was analyzed semi-quantitatively. comparison of the total viable cell count to the direct microscopic count yielded no indication that non-culturable bacteria contribute significantly to the undefined, complex ripening floras. from 2613 isolates, 48 showed clear inhibition of one or more listeria monocytogenes strains on solid medium, while only three isolates excreted an anti-listerial, soluble substance when ...	1999	10357277
effects of combined shear and thermal forces on destruction of microbacterium lacticum.	a twin-screw extruder and a rotational rheometer were used to generate shear forces in concentrated gelatin inoculated with a heat-resistant isolate of a vegetative bacterial species, microbacterium lacticum. shear forces in the extruder were mainly controlled by varying the water feed rate. the water content of the extrudates changed between 19 and 45% (wet weight basis). higher shear forces generated at low water contents and the calculated die wall shear stress correlated strongly with bacter ...	1999	10508076
aureobacterium masquerading as 'corynebacterium aquaticum' infection: case report and review of the literature.	a gram-positive bacillus was isolated repeatedly from blood taken through the lumina of a central venous catheter of a patient with multiple myeloma who developed febrile neutropenia following chemotherapy. the bacterium was identified by the api coryne system as 'corynebacterium aquaticum'. gene analysis targeting the 16s rrna indicated that the organism had a 99.5% identity with aureobacterium liquefaciens although there were two phenotypic characteristics at variance with the description of t ...	1999	10510974
microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory.	two strictly aerobic, heterotrophic and mesophilic new organisms, strains kitami a1 and kitami c2t, were isolated from the wastewater of a sugar-beet factory in kitami city, hokkaido, japan. in batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (epss) utilizing sucrose as the sole carbon source. the g + c contents of the strains kitami c2t and kitami a1 were 69.2 mol%. both strains had anteiso-c15:0 acid, anteiso-c17:0 acid and iso-c16:0 as major components. t ...	1999	10555312
facile enzymatic conversion of lactose into lacto-n-tetraose and lacto-n-neotetraose.	lacto-n-tetraose (galbeta1 -3glcnacbeta1-3galbeta1-4glc, lnt) and lacto-n-neotetraose (galbeta1-4glcnacbeta1-3galbeta1-4glc, lnnt) were enzymatically synthesized by consecutive additions of glcnac and gal residues to lactose. lacto-n-triose ii (glcnacbeta1-3galbeta1-4glc) was prepared first by the transfer of glcnac from udp-glcnac to lactose by beta-1,3-n-acetylglucosaminyltransferase from bovine serum. the resulting lacto-n-triose ii was converted into lnt and lnnt utilizing two kinds of beta- ...	1999	10596893
analysis of bacterial dna in skin and muscle of the tyrolean iceman offers new insight into the mummification process.	about 80 sequences (16s ribosomal rna gene) of bacterial dna in samples of skin and muscle taken directly from the tyrolean iceman (3350-3100 years b.c.) or recovered during the 1992 archaeological expedition at the alpine site were analyzed to obtain clues to the natural mummification process that allowed the corpse of the neolithic shepherd/hunter to be preserved for more than 5,000 years. the investigation was made more complex by the fact that the surface of the mummy had been swabbed with p ...	2000	10640948