a comparison of the susceptibility of the biting midge culicoides imicola to infection with recent and historical isolates of african horse sickness virus.the susceptibility of culicoides (avaritia) imicola kiefer (diptera: ceratopogonidae) to 21 isolates representing all nine known serotypes of african horse sickness virus (ahsv), recovered from clinical cases of the disease in south africa during 1998-2004, was compared with its susceptibility to approximately 40-year-old isolates stored at the agricultural research council-onderstepoort veterinary institute. field-collected c. imicola were fed through a chicken skin membrane on sheep blood spik ...201020626628
molecular epidemiology of african horse sickness virus based on analyses and comparisons of genome segments 7 and 10.this paper describes a method to rapidly identify african horse sickness virus (ahsv), using a single tube reverse transcription polymerase chain reaction (pcr). this method was used to amplify cdna copies of genome segments 7 and 10 from several different ahsv strains, of different serotypes, which were then analysed by sequencing and/or endonuclease digestion. ahsv vp7 (encoded by genome segment 7) is one of the two major capsid proteins of the inner capsid layer, forming the outer surface of ...19989785509
african horse sickness virus structure.african horse sickness virus (ahsv), of which there are nine serotypes (ahsv-1, -2, etc.), is a member of orbivirus genus within the reoviridae family. both in morphology and molecular constituents ahsv particles are comparable to those of bluetongue virus (btv), the prototype virus of the genus. the two viruses have seven structural proteins (vp1-7) organized in two layered capsid. the outer capsid is composed of vp2 and vp5. the inner capsid, or core, is composed of two major proteins, vp3 and ...19948001348
diagnosis and molecular epidemiology of the african horse sickness virus by the polymerase chain reaction and restriction patterns.african horse sickness is a viral disease caused by an orbivirus belonging to the reoviridae family. this paper describes a polymerase chain reaction (pcr) for amplifying segments 7, which encode for vp 7, a protein common to the 9 known serotypes of this virus. a reverse transcription step is necessary before amplification. no amplified product could be observed in cell cultures infected with other equine viruses. the amplified dnas were digested to completion by 8 different restriction enzymes ...19938260960
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