Publications

TitleAbstractYear
Filter
PMID
Filter
epidemiological aspects on vector-borne infections in stray and pet dogs from romania and hungary with focus on babesia spp.canine arthropod-borne infections are of major interest in small animal practice and have been widely investigated in central and western europe. however, only limited epidemiological data are available from south-eastern european countries, although diseases including babesiosis or dirofilariosis are widely recognised as important canine infections in these countries. a steadily increasing number of dogs imported from south-eastern europe into germany require particular attention by small anima ...201121947342
assessment of primers designed for the subspecies-specific discrimination among babesia canis canis, babesia canis vogeli and babesia canis rossi by pcr assay.canine babesiosis is an infectious disease caused by either babesia gibsoni or babesia canis protozoans. the latter is also classified under three different phylogenetic groups, referred to as subspecies b. canis canis, b. canis vogeli and b. canis rossi. the objective of the present study was to validate and standardize a pcr assay to discriminate the organisms at the subspecies level. first, the reference sequences of the 18s rrna, 5.8s rrna and 28s rrna genes, including the internal transcrib ...200818242863
detection of babesia gibsoni by reaction of phage display single chain antibodies with p50 proteins.babesia gibsoni (b. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. diagnosis of canine babesiosis is commonly carried out using giemsa-stained thin blood smears. however, at low levels of infection, it is difficult to detect babesia organisms by observation of giemsa-stained thin blood smears. we constructed a monoclonal phage display single chain antibody (scfv) against a b. gibsoni merozoite antigen, p50 protein. intraerythrocytic b. gibsoni organisms are ...200919721374
frequency and therapy monitoring of canine babesia spp. infection by high-resolution melting curve quantitative fret-pcr.babesia gibsoni and babesia canis are the etiological agents of canine babesiosis, a protozoal hemolytic disease with global significance. canine babesiosis has been diagnosed by microscopic identification of intra-erythrocytic trophozoites in blood smear, and by serological testing. here we developed a quantitative fluorescence resonance energy transfer (fret)-pcr that amplifies a fragment of the babesia spp. 18s rrna gene with high sensitivity and specificity. melting curve analysis differenti ...201019931290
the role of cystatins in tick physiology and blood feeding.ticks, as obligate hematophagous ectoparasites, impact greatly on animal and human health because they transmit various pathogens worldwide. over the last decade, several cystatins from different hard and soft ticks were identified and biochemically analyzed for their role in the physiology and blood feeding lifestyle of ticks. all these cystatins are potent inhibitors of papain-like cysteine proteases, but not of legumain. tick cystatins were either detected in the salivary glands and/or the mi ...201222647711
molecular characterizations of three distinct babesia gibsoni rhoptry-associated protein-1s (rap-1s).three cdnas encoding rhoptry-associated protein 1 (rap-1) homologues were found in the babesia gibsoni est database. based on similarities to bgrap-1a, which was identified previously by serological screening of a cdna merozoite library, the two new genes were designated bgrap-1b (33.7%) and bgrap-1c (57%). mice antiserum raised against each recombinant protein reacted specifically with b. gibsoni parasites as determined by western blotting, which showed native molecular sizes of the bgrap-1a (5 ...200919631010
babesiosis caused by a large babesia species in 7 immunocompromised dogs.a large unnamed babesia species was detected in a dog with lymphoma. it was unknown if this was an underrecognized pathogen.201020002547
molecular detection of vector-borne agents in dogs from ten provinces of china.although many vector-borne agents are potential zoonoses and cause substantial morbidity and mortality in dogs worldwide, there are limited data on these organisms in dogs of china.201526428085
development and characterization of a strain of babesia gibsoni resistant to diminazene aceturate in vitro.we attempted to develop a strain of babesia gibsoni resistant to diminazene aceturate (da), an anti-babesial drug, in vitro. since the da-sensitive b. gibsoni strain could survive and proliferate in culture medium containing 1 ng/m l da, the concentration of da was gradually increased from 1 to 200 ng/ml. the results showed that the parasites could survive and proliferate in the medium containing 200 ng/m l da, which was much higher than the 50% inhibitory concentration (ic(50)) of da for b. gib ...201020160418
a pcr study of piroplasms in 166 dogs and 111 horses in france (march 2006 to march 2008).positive pcr results for piroplasms were obtained on edta blood samples from 166 dogs and 111 horses between march 2006 and march 2008. the organisms were initially identified using common primers, followed by restriction enzyme profiles to determine the species and types of babesia/theileria. in 27 dogs and eight horses with positive results, the size of the specimen was insufficient to separate species/types. we identified babesia canis canis in 105 of the 166 dogs (63%) and theileria equi in ...201020221639
use of a doxycycline-enrofloxacin-metronidazole combination with/without diminazene diaceturate to treat naturally occurring canine babesiosis caused by babesia gibsoni.canine babesiosis is an important worldwide, tick-borne disease caused by hemoprotozoan parasites of the genus babesia. babesia gibsoni is the predominant species that causes canine babesiosis in taipei, taiwan. it is a small pleomorphic intraerythrocytic parasite that can cause erythrocyte destruction and hemolytic anemia. efficacy of oral administration of a doxycycline-enrofloxacin-metronidazole combination with and without injections of diminazene diaceturate in the management of naturally o ...201020416095
sequence and phylogenetic analysis of the thrombospondin-related adhesive protein (trap) gene of babesia gibsoni isolates from dogs in taiwan.the genetic diversity of babesia gibsoni strains worldwide is currently poorly defined. the aim of the present study was to characterize b. gibsoni strains in naturally infected dogs in taiwan using a combination of polymerase chain reaction (pcr) and sequence analysis of both 18s rdna and the gene encoding thrombospondin-related adhesive protein (trap). genomic dna was extracted from 29 parasitemic dogs, and the target genes were separately amplified, sequenced and aligned with corresponding se ...201020519852
artesunate, a potential drug for treatment of babesia infection.the effects of artesunate, a water-soluble artemisinin derivative, against babesia species, including babesia bovis, babesia gibsoni and babesia microti were studied. cultures of b. bovis and b. gibsoni were treated with 0.26, 2.6, 26 and 260microm artesunate, showing inhibition of parasite growth at concentrations equal to and greater than 2.6microm artesunate by days 3 post-treatment for b. gibsoni and b. bovis in a dose-dependent manner. consistent with in vitro experiments, artesunate was ef ...201020541037
serological and molecular prevalence of canine vector-borne diseases (cvbds) in korea.previous surveys in dogs from korea indicated that dogs are exposed to a variety of vector- borne pathogens, but perception for a nation-wide canine vector-borne disease (cvbd) occurrence has been missing. we report here results of both serological and molecular prevalence studies for major cvbds of dogs from all over the south korean peninsula except for jeju island.201728298245
babesia gibsoni: detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis.in this study, we attempted to detect babesia gibsoni in blood smears and formalin-fixed, paraffin-embedded tissues obtained from b. gibsoni-infected dogs using in situ hybridization. using a digoxigenin-conjugated deoxyribonucleic acid (dna) probe, both intraerythrocytic and exoerythrocytic parasites in the culture could be specifically stained in blood smears fixed with 4% phosphate-buffered paraformaldehyde. this indicated that genomic dna extracted from the parasites could be detected using ...201120637756
[from piroplasmosis to babesiosis--problems with classification of babesia protozoa isolated from dogs].babesia and theileria are the tick-borne parasites belonging to the order piroplasmida. both of them cause a severe disease with symptoms of hemolytic anemia. the main etiological factors of canine piroplasmosis are protozoa babesia. there are two species of this pathogen infective for dogs: babesia canis and babesia gibsoni. based on the results of molecular biology techniques it is possible to distinguish them as well as many subspecies or strains. it is known since quite some time that there ...201020707294
effects and mechanisms of action of ionophorous antibiotics valinomycin and salinomycin-na on babesia gibsoni in vitro.valinomycin and salinomycin-na, 2 ionophorous antibiotics, exhibited in vitro antibabesial activities against babesia gibsoni that infected normal canine erythrocytes containing low potassium (lk) and high sodium concentrations, i.e., lk erythrocytes, which completely lack na,k-atpase activity. the level of parasitemia of b. gibsoni was significantly decreased when the parasites were incubated in culture medium containing either 10(-1) ng/ml valinomycin or 10(2) ng/ml salinomycin-na for 24 hr. f ...200920929429
reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant babesia gibsoni variants under low concentrations of diminazene aceturate.in our previous report, we developed a diminazene aceturate (da)-resistant babesia gibsoni strain that was maintained in culture with 200 ng/ml da. while developing this strain, we also obtained da-resistant b. gibsoni variants, which were maintained in culture with da from 1 to 175 ng/ml for more than 8 weeks. because heat shock protein 70 (hsp70) seems to play important roles in adaptation to a stress environment in protozoan parasites, in the present study, we examined the copy number of b. g ...201021180255
detection of anti-babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from babesia gibsoni-infected dogs.antibodies that recognized either babesia gibsoni or canine red blood cell (rbc) 70-kilodalton (kda) protein were detected in serum from acutely and chronically b. gibsoni-infected. in those sera, antibodies that reacted with recombinant b. gibsoni and canine heat shock protein 70 (rbghsp70 and rchsp70) were detected; therefore, b. gibsoni and canine rbc 70-kda proteins seemed to be bghsp70 and chsp70, respectively. in infected dogs, the amounts of these antibodies increased after infection. int ...201121454016
evidence of new pathogenic theileria species in dogs.babesiosis, caused by tick-borne haematozoan organisms of the genus babesia, is a parasitic disease of domestic and wild mammals. canine babesidae have historically been classified as "large babesia" (babesia canis) and "small babesia" (babesia gibsoni) based on the size of their intraerythrocytic forms. recent publications, however, suggest that the diversity of piroplasm species infecting dogs might be greater than previously appreciated. sequencing and phylogenetic analysis of the ssrrna gene ...201021526030
babesiosis in dogs and cats-expanding parasitological and clinical spectra.canine babesiosis caused by different babesia species is a protozoal tick-borne disease with worldwide distribution and global significance. historically, babesia infection in dogs was identified based on the morphologic appearance of the parasite in the erythrocyte. all large forms of babesia were designated babesia canis, whereas all small forms of babesia were considered to be babesia gibsoni. however, the development of molecular methods has demonstrated that other babesia species such as ba ...201121571435
expression of truncated babesia gibsoni thrombospondin-related adhesive proteins in escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay.among the previously established enzyme-linked immunosorbent assays (elisas), an elisa using the full length of a recombinant thrombospondin-related adhesive protein of babesia gibsoni (rbgtrapf) is considered as the most sensitive diagnostic method for the detection of an antibody to b. gibsoni in dogs. however, the expression of rbgtrapf in high concentration is poor and, thus, limits its usefulness as a diagnostic antigen. to improve its expression level, we have truncated bgtrapf into two fr ...201121802417
a survey of canine tick-borne diseases in india.abstract: background: there are few published reports on canine babesia, ehrlichia, anaplasma, hepatozoon and haemotropic mycoplasma infections in india and most describe clinical disease in individual dogs, diagnosed by morphological observation of the microorganisms in stained blood smears. this study investigated the occurrence and distribution of canine tick-borne disease (tbd) pathogens using a combination of conventional and molecular diagnostic techniques in four cities in india. results: ...201121771313
possible emergence of drug-resistant variants of babesia gibsoni in clinical cases treated with atovaquone and azithromycin.there is no well-established treatment strategy for babesia gibsoni infection. a new therapeutic protocol using atovaquone (atv) and azithromycin (azm) has been proposed, but there is concern about the possible induction of relapse and the emergence of atv-resistant variants after treatment.200919645835
effects and mechanisms of action of polyene macrolide antibiotic nystatin on babesia gibsoni in vitro.abstract abstract: nystatin is a membrane-active polyene macrolide antibiotic and a channel-forming ionophore. it exhibits in vitro activity against babesia gibsoni infecting normal canine erythrocytes containing low potassium (lk) and high sodium concentrations, i.e., lk erythrocytes. the calculated ic50 value of nystatin against b. gibsoni infecting lk erythrocytes was 31.96 µg/ml. the antibabesial activity of nystatin disappeared when b. gibsoni in lk erythrocytes were incubated in culture me ...201121756054
resolution of a proteinuric nephropathy associated with babesia gibsoni infection in a dog.a 4 yr old male castrated labrador retriever was evaluated for a short history of inappetance, lethargy, small-bowel diarrhea, polyuria, and polydipsia. clinicopathologic abnormalities were consistent with protein-losing nephropathy and renal azotemia. expansive infectious disease testing implicated babesia gibsoni via whole blood polymerase chain reaction. renal histopathology results were consistent with membranoproliferative glomerulonephritis and immune complex deposition. the dog was treate ...201122058361
development of in vitro atovaquone-resistant babesia gibsoni with a single-nucleotide polymorphism in cytb.an atovaquone (atv)-resistant babesia gibsoni was developed by in vitro exposure of uncloned wild type (wt) b. gibsoni to 800nm atv for 6 days. sequence analysis of mitochondrial genes showed a single-nucleotide polymorphism (snp) at cytb nt363 (g to t) that resulted in the substitution of methionine with isoleucine (m121i), which is one of the snps reported in a previous in vivo study. 363t or 363g allele-specific real-time polymerase chain reaction (pcr) revealed that an m121i variant was pres ...201121996003
clinicopathological findings, molecular detection and characterization of babesia gibsoni infection in a sick dog from italy.a 4-year-old intact female american pit bull terrier from italy descendant of an american-born bitch was evaluated for anorexia, lethargy, weakness, and intermittent vomiting. on physical examination, the dog was dehydrated, had pale mucous membranes, hunched posture and abdominal pain. a moderate anemia was observed. splenomegaly and hyperechoic regions suspected as infarcts in the spleen were seen on abdominal ultrasound. based on the suspicion of splenic torsion, splenectomy was performed. af ...200919679397
the therapeutic efficacy of two antibabesial strategies against babesia gibsoni.various combination strategies for treating babesia gibsoni have been described. however, relapses after administering some combinations of antibabesial drugs and the presence of drug-resistant b. gibsoni still pose significant challenges to veterinarians. to compare the efficacy of a combination of clindamycin, diminazene, and imidocarb (cdi) to that of a combination of atovaquone and azithromycin (aa) for the treatment of b. gibsoni and to correlate drug efficacy with b. gibsoni mutations, 30 ...201122222008
molecular cloning, phylogenetic analysis and heat shock response of babesia gibsoni heat shock protein 90.the babesia gibsoni heat shock protein 90 (bghsp90) gene was cloned and sequenced. the length of the gene was 2,610 bp with two introns. this gene was amplified from cdna corresponding to full length coding sequence (cds) with an open reading frame of 2,148 bp. a phylogenetic analysis of the cds of hsp90 gene showed that b. gibsoni was most closely related to b. bovis and babesia sp. bq1/lintan and lies within a phylogenetic cluster of protozoa. moreover, mrna transcription profile for bghsp90 e ...201627149891
theileria annae in a young swedish dog.a severe regenerative anemia was detected in a 12-week-old mixed breed puppy in sweden. a small protozoan parasite was observed in erythrocytes on a blood smear. it was initially suspected to be babesia gibsoni based on its size and because b. gibsoni was previously recorded in sweden. surprisingly, specific polymerase chain reaction analysis identified the protozoan as theileria annae. t. annae is endemic in northwest spain, is very uncommonly reported elsewhere and has never been recorded in s ...201323842507
a six year (2006-2011) retrospective study of hemoprotozoan parasites affecting dogs in chennai, tamil nadu, india.with the changing global scenario there is effect not only on the human survival but also on the prevalence of existing pathogens and appearance of new infectious agents among animal population. this paper emphasises the need for prevalence studies. a retrospective study for 6 years on prevalence of hemoprotozoans in dogs in chennai showed babesia gibsoni the predominant parasite of dogs. details are discussed in this paper.201224808651
molecular investigation of tick-borne pathogens in dogs from luanda, angola.no molecular data have been available on tick-borne pathogens that infect dogs from angola. the occurrence of agents from the genera anaplasma, babesia, ehrlichia and hepatozoon was assessed in 103 domestic dogs from luanda, by means of the polymerase chain reaction (pcr) and dna sequence analysis.201627160839
canine babesiosis in northwestern india: molecular detection and assessment of risk factors.in the current study, a total of 214 blood samples from dogs in and around ludhiana, punjab (india), suspected for canine babesiosis were examined with conventional and molecular assays. examination of giemsa-stained peripheral thin blood smears revealed an overall prevalence of 7.47% (16/214) for canine babesiosis encompassing 0.93% (2/214) of large babesia and 6.54% (14/214) of babesia gibsoni. however, molecular diagnosis revealed 15.42% (33/214) samples positive for b. gibsoni infection as e ...201425013798
molecular survey of babesia gibsoni using haemaphysalis longicornis collected from dogs and cats in japan.a nationwide survey of babesia gibsoni using haemaphysalis longicornis collected from dogs and cats in japan was conducted using molecular methods. a total of 1,341 h. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by b. gibsoni-targeted pcr. partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from tottori, hiroshima, yamaguchi, tokushima, ehime and oita prefectures (all in western ...201424920547
first report of babesia gibsoni in central america and survey for vector-borne infections in dogs from nicaragua.although many vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas and potential zoonoses, there is little information on these conditions in central america.201424667065
prevalence of vector-borne pathogens in dogs from haiti.canine vector-borne pathogens are common on some caribbean islands, but survey data in haiti are lacking. to determine the prevalence of selected vector-borne pathogens in dogs from haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with rhipicephalus sanguineus ticks at the time of blood collection. no other tick species were identified on these dogs. a commercially available elisa identified antibodies to ehrlichia spp. in 69 (32.9%), antibodies to ...201627270383
first molecular detection of tick-borne pathogens in dogs from jiangxi, china.in this study, blood samples obtained from 162 dogs in jiangxi, china, were employed in molecular screening of canine tick-borne pathogens by pcr and sequencing. babesia spp. gene fragment was detected in 12 (7.41%) dogs. all samples were negative for hepatozoon spp., ehrlichia canis, coxiella spp., borrelia spp., rickettsia spp. and anaplasma platys. species-specific pcr analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by babesia canis vogeli and babesia gibsoni, respe ...201727890889
first detection and molecular identification of babesia gibsoni from rhipicephalus sanguineus ticks.babesia gibsoni was firstly identified in rhipicephalus sanguineus ticks infested on dogs in asia. a total of 1195 ticks collected from veterinary practitioners and pet clinics were examined for babesia by nested-polymerase chain reaction assay based on the 18s small subunit ribosomal rna (ssrrna) gene. babesia infection was detected in nymph, male and female ticks with an infection rate of 2.42%, 0.98% and 1.97%, respectively. sequence and phylogenetic analysis revealed that these babesia spp. ...201727686959
molecular detection of anaplasma platys and babesia gibsoni in dogs in malaysia.this study reports for the first time molecular detection of anaplasma platys infection in 4 (13.3%) of 30 malaysian dogs investigated. a low occurrence (3.3%) of babesia gibsoni was also noted, being detected in one of the 30 dogs. rickettsia, bartonella, orientia tsutsugamushi, and ehrlichia dna were not detected in the dog blood samples. the role of a. platys as an agent of canine anaplasmosis and its transmission through rhipicephalus sanguineus ticks merits further investigation.201323959500
tick-borne pathogens and disease in dogs on st. kitts, west indies.between 2009 and 2011, we conducted a case-control study of ticks and tick-associated pathogens affecting dogs on the island of st. kitts, eastern caribbean, including 55 cases of clinically suspected tick-borne disease (tbd) and 110 presumably healthy animals presented for elective surgeries. rhipicephalus sanguineus caused year-round infestations of dogs, and 36% of the dogs in the study were infested at the time of examination. overall, 62% of suspected tbd cases and 24% of presumably healthy ...201323481028
improvement of the cryopreservation method for the babesia gibsoni parasite by using commercial freezing media.in vitro cultivation and cryopreservation under liquid nitrogen have already been reported and established for babesia bovis and babesia bigemina parasites. although the in vitro cultivation methods for babesia gibsoni have been reported and established, the cryopreservation methods for this parasite have not been established completely. in this paper, we compared several freezing media for the cryopreservation of b. gibsoni parasite. the cellbanker® series (1 plus and 2), stem-cellbanker®, and ...201626921519
molecular characterization and antigenic properties of a novel babesia gibsoni glutamic acid-rich protein (bggarp).identification and molecular characterization of babesia gibsoni proteins with potential antigenic properties are crucial for the development and validation of the serodiagnostic method. in this study, we isolated a cdna clone encoding a novel b. gibsoni 76-kda protein by immunoscreening of the parasite cdna library. computer analysis revealed that the protein presents a glutamic acid-rich region in the c-terminal. therefore, the protein was designated as b. gibsoni glutamic acid-rich protein (b ...201323968686
inhibitory effect of cyclophilin a from the hard tick haemaphysalis longicornis on the growth of babesia bovis and babesia bigemina.haemaphysalis longicornis is known as one of the most important ticks transmitting babesia parasites in east asian countries, including babesia ovata and babesia gibsoni, as well as theileria parasites. h. longicornis is not the natural vector of babesia bovis and babesia bigemina. vector ticks and transmitted parasites are thought to have established unique host-parasite interaction for their survival, meaning that vector ticks may have defensive molecules for the growth control of parasites in ...201323532543
molecular characterization of babesia bovis m17 leucine aminopeptidase and inhibition of babesia growth by bestatin.the m17 leucine aminopeptidase (m17lap) enzymes of the other apicomplexan parasites have been characterized and shown to be inhibited by bestatin. though babesia bovis also belongs to the apicomplexan group, it is not known whether its m17lap could display similar biochemical properties as well as inhibition profile. to unravel this uncertainty, a b. bovis m17lap (bbm17lap) gene was expressed in escherichia coli , and activity of the recombinant enzyme as well as its inhibition by bestatin were ...201526057618
the epidemiological survey for atovaquone resistant related gene of babesia gibsoni in japan.in 73 gdna samples from babesia gibsoni-infected dogs, the m121i variant population was measured by using allele-specific real-time pcr. although the mechanism of atovaquone against b. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant b. gibsoni had a single-nucleotide substitution at nt363 (g to t), which resulted in the substitution of methionine with isoleucine (m121i). in this study, 3/73 samples showed over 5% m121i v ...201626549436
mycophenolic acid, mycophenolate mofetil, mizoribine, ribavirin, and 7-nitroindole inhibit propagation of babesia parasites by targeting inosine 5'-monophosphate dehydrogenase.the resistance of babesia parasites to current anti-babesiosis drugs is an issue of major concern. the inosine 5'-monophosphate dehydrogenase (impdh) of babesia gibsoni has been identified and characterized as a molecular drug target in our previous studies. in the present study, inhibitory effects of impdh inhibitors (mycophenolate mofetil, mizoribine, ribavirin, 7-nitroindole, and mycophenolic acid) were evaluated in vitro or in vivo. in the inhibition assay of recombinant b. gibsoni impdh act ...201424580148
canine tick-borne diseases in pet dogs from romania.tick-borne diseases are of substantial concern worldwide for animals as well as humans. dogs have been a human companion for millennia, and their significant impact on human life renders disease in dogs to be of great concern. tick-borne diseases in dogs represent a substantial diagnostic challenge for veterinarians in that clinical signs are often diffuse and overlapping. in addition, co-infections with two or more pathogens enhance this problem further. molecular methods are useful to disentan ...201728335825
phytoecdysteroids as modulators of the toxoplasma gondii growth rate in human and mouse cells.searching for new effective drugs against human and animal toxoplasmosis we decided to test the anti-toxoplasma potential of phytoecdysteroids (α-ecdysone and 20-hydroxyecdysone) characterized by the pleiotropic activity on mammalian organisms including the enhancement of host's anti-parasitic defence. this objective was accomplished by the in vitro evaluation of t. gondii growth in phytoecdysteroid-treated immunocompetent cells of selected hosts: humans and two strains of inbred mice with genet ...201526272689
black-backed jackals (canis mesomelas) are natural hosts of babesia rossi, the virulent causative agent of canine babesiosis in sub-saharan africa.babesia rossi, which is transmitted by haemaphysalis spp. and is highly virulent to domestic dogs, occurs only in sub-saharan africa. since dogs are not native to the region, it has been postulated that the natural host of b. rossi is an indigenous african canid. although various attempts at artificial infection indicated that black-backed jackals (canis mesomelas) could become subclinically infected with b. rossi, data on occurrence of b. rossi in free-ranging jackals was lacking. a long-term b ...201728285591
molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease.the spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (vbp), which could have a fundamental role in the pathogenesis of splenic disease. this study aimed to evaluate certain vbp in samples from dogs with splenic lesions.201728285583
Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family.We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding native proteins belong to merozoite surface protein family of B. gibsoni (BgMSP). Moreover, antisera against either rBgP45 or rBgP47 cross-reacted with all the proteins of the BgMSP family on ELISA and IFAT analyses. Of the four candidate antigens, ELISA with rBgP45 yielded high se ...201122172478
phylogenetic characterization of babesia canis vogeli in dogs in the state of goiás, brazil.the genus babesia comprises protozoa that cause diseases known as babesiosis. dogs are commonly affected by babesia canis or babesia gibsoni. babesia canis is divided into the subspecies babesia canis canis, babesia canis vogeli and babesia canis rossi. among these, babesia canis vogeli predominates in brazil. the objective of this study was to conduct a phylogenetic analysis on babesia isolates from dogs in goiânia, goiás. blood samples were obtained from 890 dogs presenting clinical signs sugg ...201122166380
development of loop-mediated isothermal amplification (lamp) for detection of babesia gibsoni infection in dogs.diagnosis of canine babesiosis, caused by babesia gibsoni is difficult, especially in chronically infected dogs. a loop mediated isothermal amplification (lamp) assay was developed and standardized by using four oligonucleotide primers targeting the hypervariable region of 18s rrna gene (genbank acc. no. kc461261). the primers specifically amplified b. gibsoni dna, while no amplification was detected with dna from non-infected dogs as well as from dogs infected with babesia canis vogeli, hepatoz ...201525749021
distribution patterns of babesia gibsoni infection in hunting dogs from nine japanese islands.canine babesiosis constitutes a major global veterinary medical problem caused by tick-borne hemoparasites babesia gibsoni and babesia canis. babesia gibsoni induces more severe clinical signs and is mainly transmitted by the ixodid haemaphysalis longicornis. in japan, b. gibsoni is primarily found in the western districts, with few records in the eastern parts. the aim of the current investigation was to evaluate distribution patterns of b. gibsoni infection in 9 japanese islands and peninsulas ...201525419881
development and evaluation of serodiagnostic assays with recombinant bgsa1 of babesia gibsoni.indirect elisa, dot-elisa and double antibody sandwich elisa (das-elisa) using truncated recombinant bgsa1 (rbgsa1) were developed for detecting babesia gibsoni infection in naturally infected dogs. truncated bgsa1 gene of 858 bp, encoding 32 kda protein was cloned in pet-32a(+) expression vector, expressed in escherichia coli and the recombinant protein was purified under native conditions. to evaluate the ability of the truncated rbgsa1as serodiagnostic reagent for b. gibsoni infection, a pane ...201425241636
efficacy of malarone(®) in dogs naturally infected with babesia gibsoni.the efficacy of malarone(®) alone and in combination with doxycycline (doxy) against babesia gibsoni infections was examined in 8 dogs. in all dogs except one treated with malarone(®), parasitemia decreased, and anemia improved soon after initiation of treatment. however, 3 of 4 dogs treated with malarone(®) relapsed, and relapse was inhibited in 2 of 4 dogs treated with malarone(®) and doxy. all relapsed dogs responded well to the second treatment, but 1 dog relapsed again and did not respond t ...201424909969
protective efficacy of babesia gibsoni culture-derived exoantigens against the challenge infection in dogs.the aim of this study is to determine the efficacy of exoantigens derived from babesia gibsoni cultures to induce protective immunity against challenge exposure of virulent organisms. an attenuated b. gibsoni oita strain was maintained in vitro by the microaerophilus stationary phase (masp) method, and exoantigens-containing supernatant fluids were collected for preparation of the immunization. two dogs received three subcutaneous immunizations with a 20-day interval of b. gibsoni exoantigens pl ...201424535736
prime-boost vaccination with plasmid dna followed by recombinant vaccinia virus expressing bggarp induced a partial protective immunity to inhibit babesia gibsoni proliferation in dogs.a heterologous prime-boost vaccination regime with dna and recombinant vaccinia virus (rvv) vectors expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. in this study, we describe the effectiveness of the prime-boost strategy by immunizing dogs with a recombinant plasmid followed by vaccinia virus, both of which expressed the glutamic acid-rich protein (bggarp) of babesia gibsoni. the dogs immunized with the prime-boost regime dev ...201324338330
genetic characterization and phylogenetic relationships based on 18s rrna and its1 region of small form of canine babesia spp. from india.canine babesiosis is a vector borne disease caused by intra-erythrocytic apicomplexan parasites babesia canis (large form) and babesia gibsoni (small form), throughout the globe. apart from few sporadic reports on the occurrence of b. gibsoni infection in dogs, no attempt has been made to characterize babesia spp. of dogs in india. fifteen canine blood samples, positive for small form of babesia, collected from northern to eastern parts of india, were used for amplification of 18s rrna gene (∼16 ...201425120099
optimization of a molecular method for the diagnosis of canine babesiosis.babesiosis is a hemolytic disease caused by protozoans of the genus babesia (apicomplexa). this disease occurs worldwide and is transmitted by ticks to a variety of mammals, including humans. the objective of the present study was to optimize a molecular approach for the detection of a fragment of 18s rdna of babesia canis, babesia vogeli, babesia rossi or babesia gibsoni based on a single semi-nested polymerase chain reaction (pcr), and compare the efficiency of this approach with that of a sim ...201424728371
actin polymerization mediated by babesia gibsoni aldolase is required for parasite invasion.host cell invasion by apicomplexan parasites driven by gliding motility and empowered by actin-based movement is essential for parasite survival and pathogenicity. the parasites share a conserved invasion process: actin-based motility led by the coordination of adhesin-cytoskeleton via aldolase. a number of studies of host cell invasion in the plasmodium species and toxoplasma gondii have been performed. however, the mechanisms of host cell invasion by babesia species have not yet been studied. ...201323792005
survey of babesiosis in symptomatic dogs from romania: occurrence of babesia gibsoni associated with breed.blood samples from 49 symptomatic dogs from 5 western and north-western counties of romania were screened using microscopic examination, polymerase-chain-reaction-restriction-fragment-length-polymorphism procedure (pcr-rflp), and sequence analysis. results of the microscopic evaluation of stained blood smears revealed 45 positive samples with the presence of large and small intraerythrocytic piroplasms in 35 and 10 samples, respectively. babesia canis (35/49, 71.4%) and babesia gibsoni (14/49, 2 ...201323994336
identification of babesia species infecting dogs using reverse line blot hybridization for six canine piroplasms, and evaluation of co-infection by other vector-borne pathogens.canine infection by vector-borne hemoparasites is frequent in tropical and sub-tropical areas where exposure to hematophageous ectoparasites is intensive. a reverse line blot (rlb) assay was designed to improve the simultaneous detection of all named canine piroplasm species combined with other vector-borne pathogens of dogs including ehrlichia canis, hepatozoon canis and leishmania infantum common in the mediterranean basin. blood samples of 110 dogs from spain (n=21), portugal (n=14) and israe ...201323017370
a unique case of babesia gibsoni infected dog with paraplegia.a 4 year old male rottweiler was presented to teaching veterinary clinics of gadvasu with a primary complaint of paraplegia, fever and inappetence. the dog was found to be dull with elevated rectal temperature (105 °f), heart rate (158 per minute) and respiration rate (57 per minute). blood smear examination and pcr assay revealed that dog was positive for babesia gibsoni. elevated bilirubin, alanine aminotransferase, alkaline phosphatase, creatinine, total leucocyte count, neutrophils and hypog ...201627876993
critical analysis of vector-borne infections in dogs: babesia vogeli, babesia gibsoni, ehrlichia canis and hepatozoon canis in punjab, india.there are few published studies on various vector borne diseases of dogs in india and most depict clinical infection in dogs, diagnosed by observation of the haemopathogens in stained blood smears. this study provides the first report regarding molecular confirmation and ancestral relationship analysis of blood smears positive cases of assorted haemopathogens in punjab province of india. on blood smear examination, haemopathogens were observed in 124 out of 778 (15.95%, 95% ci: 13.53- 18.68) blo ...201627787221
infectious diseases in dogs rescued during dogfighting investigations.dogs used for dogfighting often receive minimal preventive health care, and the potential for spread of infectious diseases is high. the purpose of this study was to describe the prevalence of infectious diseases in dogs rescued from fighting operations to guide medical protocols for their immediate and long-term care. a total of 269 pit bull-type dogs were seized in a multi-state investigation. fleas were present on most dogs, but few ticks were observed. testing performed at intake included pa ...201627056107
molecular detection and genetic diversity of babesia gibsoni in dogs in india.babesia gibsoni is a tick borne intraerythrocytic protozoan parasite causing piroplasmosis in dogs and has been predominantly reported in asian countries, including japan, korea, taiwan, malaysia, bangladesh and india. the present communication is the first evidence on the genetic diversity of b. gibsoni of dogs in india. blood samples were collected from 164 dogs in north and northeast states of india and 13 dogs (7.9%) were found positive for b. gibsoni infection by microscopic examination of ...201627020545
a simpleprobe(®) real-time pcr assay for differentiating the cytochrome b m121i mutation in clinical specimens from dogs infected with babesia gibsoni.babesia gibsoni (b. gibsoni) causes a canine tick-borne disease worldwide. the substitution of methionine with isoleucine (m121i) in the cytochrome b (cytb) gene of b. gibsoni was identified as being associated with atovaquone resistance. rapid identification of the drug-resistant strain is required to select a more effective combination of drugs, e.g., from atovaquone and azithromycin (aa) to clindamycin, diminazene, and imidocarb (cdi) combination. a simpleprobe(®) real-time pcr assay was desi ...201626874668
babesia gibsoni internal transcribed spacer 1 region is highly conserved amongst isolates from dogs across japan.babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia with highly variable clinical outcome. in this study, we sequenced the 254bp internal transcribed spacer 1 region (its1) of 54 b. gibsoni isolates from 14 different geographical regions of japan. the 54 isolates shared high sequence identity with each other and with b. gibsoni isolates reported in genbank database (97.2-100%). consistent with previous reports, phylogenetic analysis showed t ...201626806537
a study of naturally acquired canine babesiosis caused by single and mixed babesia species in zambia: clinicopathological findings and case management.a retrospective and prospective analysis of clinical records of dogs diagnosed with babesia infections was carried out for the years 2000 to 2013 from practices in lusaka, zambia. records of 363 dogs with confirmed babesia infections were analysed using demographic factors including sex, breed, age, and clinical signs in relation to haematological findings and babesia species. the clinical and laboratory findings observed are described as well as babesia species identification. the study include ...201526682062
genetic variations of four immunodominant antigens of babesia gibsoni isolated from dogs in southwest japan.babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia. previous reports have shown that the apical membrane antigen 1 (bgama1), the 50 kda surface antigen (bgp50), the secreted antigen 1 (bgsa1), and the thrombospondin-related adhesive protein (bgtrap) are promising diagnostic antigens and vaccine candidates against b. gibsoni. in the present study, we investigated the genetic polymorphisms and natural selection of these four genes of b. gibso ...201626615873
seroprevalence survey of babesia gibsoni infection and tick species in dogs in east china.a seroprevalence survey of babesia gibsoni infection in dogs in east china was conducted using an elisa with recombinant b. gibsoni thrombospondin-related adhesive protein (bgtrap). a total of 1170 dogs from east china were examined and the seroprevalence was 9.23%. the proportion of samples was 81.2%, 7.86% and 10.94% from pet, working and fighting dogs, respectively. the fighting dogs showed highest seroprevalence (39.8%) compared with working dogs (26.1%) and pet dogs (3.47%). these results i ...201526483164
clinical, haematological, cytokine and acute phase protein changes during experimental babesia gibsoni infection of beagle puppies.babesia gibsoni is a haemoprotozoan parasite of emerging global importance. the clinical presentation of babesial infections is diverse and the systemic inflammatory response induced by infection is considered to be a major feature of the pathophysiology of canine babesiosis. an experimental case-controlled longitudinal study was conducted to assess the clinical, haematological, cytokine and acute phase protein changes that occur during experimental b. gibsoni infection of beagle puppies. infect ...201526297954
a molecular epidemiological survey of babesia, hepatozoon, ehrlichia and anaplasma infections of dogs in japan.tick-borne diseases are often encountered in canine clinical practice. in the present study, a molecular epidemiological survey of dogs in japan was conducted to understand the prevalence and geographical distribution of babesia spp., hepatozoon spp., ehrlichia spp. and anaplasma spp. pathogen-derived dna in blood samples obtained from 722 dogs with a history of exposure to ticks and/or fleas was examined by pcr. the prevalence of babesia gibsoni, babesia odocoilei-like species, hepatozoon canis ...201525947226
the presence of tick-borne diseases in domestic dogs and cats living on iriomote-jima and tsushima islands.the iriomote cat and tsushima leopard cat are endangered wildcats in japan and inhabit only iriomote-jima and tsushima islands, respectively. domestic dogs and cats living on iriomote-jima and tsushima islands were surveyed to clarify the interrelationship between wildcats and domestic animals regarding tick-borne disease transmission. pathogen-derived dna in blood samples was detected by polymerase chain reaction. babesia gibsoni was detected in dogs of iriomote-jima, and hepatozoon felis and h ...201728484147
canine babesiosis in china caused by babesia gibsoni: a molecular approach.to provide a point of reference to study the epidemiology and clinical expression of canine babesiosis in china.201725848381
identification of the babesia-responsive leucine-rich repeat domain-containing protein from the hard tick haemaphysalis longicornis.haemaphysalis longicornis is a tick known for transmitting babesia parasites, including babesia gibsoni, in east asian countries. the vector tick must have strategies to control babesia parasites, while babesia parasites are also considered to establish an evasive mechanism from the tick's innate immunity. due to this mutual tolerance, h. longicornis is considered to be a vector of babesia parasites. recent studies have shown the important roles of leucine-rich repeat (lrr) domain-containing pro ...201525690462
development of recombinant bgp12 based enzyme linked immunosorbent assays for serodiagnosis of babesia gibsoni infection in dogs.indirect elisa and dot-elisa using recombinant bgp12 (rbgp12) were developed for the diagnosis of babesia gibsoni infected dogs. the complete open reading frame of bgp12 gene (378bp) was cloned in pet-32a(+) expression vector and expressed in escherichia coli as a soluble thioredoxin (trx) fusion protein. the purified rbgp12 was used for production of anti-rbgp12 rabbit serum, which recognized a native 12-kda protein in b. gibsoni infected erythrocyte by western blot analysis. to evaluate the po ...201626827835
first molecular identification of babesia gibsoni in dogs from slovakia, central europe.canine babesiosis is a severe and potentially life threatening infection. in europe, babesia canis is considered to be the most common species responsible for the disease. we report two cases of babesiosis caused by babesia gibsoni. the polymerase chain reaction, restriction fragment length polymorphism analysis and further sequencing of 18s rrna gene fragments from blood samples of both dogs revealed the identity of isolates with b. gibsoni genotypes from other dogs worldwide. this species was ...201626304014
clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from serbia.canine babesiosis is a frequent and clinically significant tick-borne disease. sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in serbia. after clinical examination, blood samples were taken for microscopic examination, complete blood count (cbc), canine snap 4dx test, dna analyses and sequencing. the main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, ...201526051258
in vitro and in vivo safety and efficacy studies of amphotericin b on babesia gibsoni.babesia gibsoni is a causative pathogen of canine babesiosis, which is commonly treated with anti-babesial drugs; however, the development of novel, more effective anti-babesial drugs is necessary because the currently used drugs cannot remove the parasites from dogs. therefore we investigated the anti-babesial effect of amphotericin b (amb), a membrane-active polyene macrolide antibiotic. the interaction of such compounds with sterols in bilayer cell membranes can lead to cell damage and ultima ...201425260334
new molecules in babesia gibsoni and their application for diagnosis, vaccine development, and drug discovery.babesia gibsoni is an intraerythrocytic apicomplexan parasite that causes piroplasmosis in dogs. b. gibsoni infection is characterized clinically by fever, regenerative anemia, splenomegaly, and sometimes death. since no vaccine is available, rapid and accurate diagnosis and prompt treatment of infected animals are required to control this disease. over the past decade, several candidate molecules have been identified using biomolecular techniques in the authors' laboratory for the development o ...201425246713
the in vitro interactions and in vivo efficacy of atovaquone and proguanil against babesia gibsoni infection in dogs.in vitro interactions between atovaquone (atv) and proguanil (pg) against babesia gibsoni and the clinical efficacy of this combination therapy using malarone(®) which is the antimalarial drug containing atv and pg were evaluated. this combination showed synergism against uncloned wild-type and atv-resistant b. gibsoni in vitro examinations using a modified fixed ratio method. administration of malarone(®) to experimentally b. gibsoni infected two dogs in chronic stage and three dogs in acute st ...201324075418
cloning and characterization of a 2-cys peroxiredoxin from babesia gibsoni.peroxiredoxins (prxs) are a family of antioxidant enzymes. here, we cloned a 2-cys prx, bgtpx-1, from the canine babesia parasite b. gibsoni. sequence identity between bgtpx-1 and 2-cys prx of b. bovis was 81% at the amino acid level. enzyme activity assay by using recombinant bgtpx-1 (rbgtpx-1) indicated that bgtpx-1 has antioxidant activity. antiserum from a mouse immunized with rbgtpx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kda and also a 44 kda protei ...201424025459
virulence attenuation of babesia gibsoni by serial passages in vitro and assessment of the protection provided by the immunization against the passaged isolate in dogs.the virulence of the babesia gibsoni oita isolate was attenuated by serial passages in vitro by using the microaerophilus stationary phase (masp) technique. after 400 serial passages, the virulence of the isolate was found to be attenuated. this was evidenced by the response of two dogs inoculated intravenously with 10(9)b. gibsoni passaged isolate. specific antibodies were produced at a titer of 1:20,480, as detected by the fluorescent antibody test (ifat). these results suggested that the seri ...201323773722
generalized alopecia with vasculitis-like changes in a dog with babesiosis.a locally bred, 12-year-old, intact female satsuma dog presented with generalized alopecia. erythema, crusts and desquamation were observed primarily on the truck. papules and erosions were present in the pinnae, and there were multiple areas of skin necrosis on the right forelimb. the cutaneous lesions had not responded to treatment with systemic antibiotics and prednisolone. the dog also had progressive anemia. babesia gibsoni was detected in the blood, and the dog was treated with antiprotozo ...201323676299
cloning, characterization and validation of inosine 5'-monophosphate dehydrogenase of babesia gibsoni as molecular drug target.the inosine monophosphate dehydrogenase (impdh) enzyme has been characterized and validated as a molecular drug target in other apicomplexans but not in the genus babesia. subsequently, we cloned and expressed a babesia gibsoni impdh (bgimpdh) cdna in escherichia coli. we also determined the inhibitory effect of mycophenolic acid (mpa) on recombinant bgimpdh (rbgimpdh) activity and the babesia-growths in vitro. the translated bgimpdh peptide contained thirteen amino acid residues responsible for ...201323142571
analysis of energy generation and glycolysis pathway in diminazene aceturate-resistant babesia gibsoni isolate in vitro.in our previous study, the level of parasitemia of the diminazene aceturate (da)-resistant b. gibsoni isolate was continuously lower than that of the wild-type, indicating the possible alteration of energy metabolism in that isolate. therefore, in the present study, the concentrations of atp, glucose, lactate, and pyruvate, and the activities of lactate dehydrogenase and pyruvate kinase in the wild-type and da-resistant isolate of b. gibsoni were measured and compared to investigate the amount o ...201223094580
molecular characterization and phylogenetic analysis of babesia sp. nv-1 detected from wild american mink ( neovison vison ) in hokkaido, japan.babesiosis is a tick-borne protozoan disease affecting many mammalian species worldwide, caused by the intraerythrocytic multiplication of babesia spp. the present study aimed to detect the presence of babesia sp. in 13 american mink from hokkaido, japan. one of 13 animals was positive, as indicated by nested pcr targeting the 18s ribosomal rna (ssu rdna) and subunit 7 (eta) of the chaperonin-containing t-complex polypeptide 1 (cct7) genes from species of babesia and theileria. sequencing of the ...201322994135
quantitative pcr-based parasite burden estimation of babesia gibsoni in the vector tick, haemaphysalis longicornis (acari: ixodidae), fed on an experimentally infected dog.most causative agents of babesiosis, babesia parasites, are transmitted transovarially in ixodid ticks. in this study, b. gibsoni, the causative agent of canine babesiosis which has transovarial transmission, was detected in tissues of the vector tick, haemaphysalis longicornis using a modified quantitative pcr assay. conventional pcr results showed that the newly designed primer set, which amplifies a 143-bp fragment of rhoptry-associated protein-1 (bgrap-1) gene in b. gibsoni, was 100 times mo ...201322878543
development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of babesia gibsoni.we developed an immunochromatographic test (ict) with the full-length of thrombospondin-related adhesive protein of babesia gibsoni expressed by the modified expression method. the developed ict showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ict could be a new practical diagnostic test for b. gibsoni infection.201222795671
involvement of mitochondrial genes of babesia gibsoni in resistance to diminazene aceturate.the stability of the characteristics of the diminazene aceturate (da)-resistant b. gibsoni isolate was initially determined in vitro. part of the da-resistant b. gibsoni isolate was cultured without da for 4 weeks, and then newly exposed to 200 ng/ml da. as a result, this isolate could proliferate the same as the da-resistant isolate, indicating that the characteristic of da resistance was stable in the da-resistant isolate. additionally, the level of parasitemia in the da-resistant isolate was ...201222673639
molecular epidemiological survey of the babesia gibsoni cytochrome b gene in western japan.in this study, we conducted a survey of the cytochrome b (cytb) gene of babesia gibsoni (b. gibsoni) isolated from clinical cases to determine the prevalence of potential atovaquone (atv)-resistant variants. ninety-two blood samples were collected from naturally b. gibsoni infected dogs. the cytb nucleotide sequence was determined by direct sequencing. twelve non-synonymous amino acid substitutions were identified in cytb. the principal atv-resistant substitution, m121i, was detected in three ca ...201222672842
molecular detection of vector-borne pathogens in dogs and cats from qatar.vector-borne diseases (vbds) have been increasingly reported in dogs and cats worldwide. however, no data are currently available regarding canine and feline vbds in qatar and limited information is available from other persian gulf countries.201728633666
transient transfection of intraerythrocytic babesia gibsoni using elongation factor-1 alpha promoter.the development of gene manipulation techniques has been reported in many protozoan parasites over the past few years. however, these techniques have not yet been established for babesia gibsoni. here, we report for the first time, the successful transient transfection of b. gibsoni. the plasmid containing the firefly luciferase reporter gene (pbs-ela) was transfected into b. gibsoni by an amaxa 4d nucleofector™ device. transfection using program fa113 and lonza buffer sf showed the highest luci ...201728729071
efficacy of azithromycin and compounded atovaquone for treatment of babesia gibsoni in dogs.approximately one-third of dogs confiscated during dogfighting investigations are infected with babesia gibsoni. traditional management of b. gibsoni with polymerase chain reaction (pcr)-screening, treatment with commercially available azithromycin and atovaquone, and pcr testing after 60 and 90 days is costly and impractical for large numbers of dogs at a time.201728625019
detection and molecular characterization of babesia, theileria, and hepatozoon species in hard ticks collected from kagoshima, the southern region in japan.to reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (pcr) system to detect the most common agents of tick-borne parasitic diseases, namely babesia, theileria, and hepatozoon parasites. we collected host-seeking or animal-feeding ticks in kagoshima prefecture, the southernmost region of kyusyu island in southwestern japan. twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for ...201728501503
molecular detection and genetic diversity of babesia gibsoni in dogs in bangladesh.babesia gibsoni is a tick-borne hemoprotozoan parasite of dogs that often causes fever and hemolytic illness. detection of b. gibsoni has been predominantly reported in asian countries, including japan, korea, taiwan, malaysia, bangladesh and india. the present study shows the first molecular characterization of b. gibsoni detected from dogs in bangladesh. blood samples were collected on fta® elute cards from 50 stray dogs in mymensingh district in bangladesh. dna eluted from the cards was subje ...201525620376
the efficacy of artemisinin, artemether, and lumefantrine against babesia gibsoni in vitro.artemisinin has many derivatives, and it is effective against plasmodium spp. however, only a limited number of reports have confirmed the efficacy of artemisinin derivatives against babesia spp. in this study, whether artemisinin and artemether could inhibit the growth of babesia gibsoni was evaluated in vitro. in addition, the interaction between artemether and lumefantrine was evaluated. these drugs inhibited the growth of b. gibsoni, but artemisinin and artemether showed lower sensitivity ag ...201525523292
Displaying items 201 - 300 of 300