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optimizing the efficacy of paecilomyces lilacinus (strain 251) for the control of root-knot nematodes.the egg pathogenic fungus paecilomyces lilacinus (strain 251) is a biocontrol fungus with a potential range of activity to control the worldwide most important plant parasitic nematodes. this biological nematicide may be an useful tool in an integrated approach to control mainly sedentary nematodes. greenhouse experiments were conducted with the root-knot nematodes meloidogyne incognita and m. hapla on tomato. p. lilacinus, formulated as wg (bioact wg), was incorporated into soil inoculated with ...200415759437
[the effect of hydrogen peroxide on the growth of microscopic mycelial fungi isolated from habitats with different levels of radioactive contamination].the effect of hydrogen peroxide ( 10(-9)-10(-1) m) on the mycelial growth of the fungi alternaria alternata, cladosporium cladosporioides, mucor hiemalis, and paecilomyces lilacinus has been studied. the growth of fungi isolated from habitats with a background level of radioactive contamination was stopped by h2o2 concentrations equal to 10(-3) and 10(-2) m, whereas the growth of fungi that were isolated from habitats with high levels of radioactive contamination was only arrested by 10(-1) m h2 ...200516400985
carbon requirements of some nematophagous, entomopathogenic and mycoparasitic hyphomycetes as fungal biocontrol agents.thirty-three carbon sources were evaluated for their effects on spore germination, hyphal growth and sporulation of 11 fungal biocontrol agents, i.e. the nematophagous fungi paecilomyces lilacinus, pochonia chlamydosporia, hirsutella rhossiliensis, h. minnesotensis and arkansas fungus 18, the entomopathogenic fungi lecanicillium lecanii, beauveria bassiana and metarhizium anisopliae, and the mycoparasitic fungus trichoderma viride. variations in carbon requirements were found among the fungal sp ...200616649079
paecilomyces lilacinus scleritis with secondary keratitis.to report a case of paecilomyces lilacinus scleritis with secondary keratitis after initially diagnosed immune-based scleritis.200717251822
effect of population dynamics of pseudomonas cepacia and paecilomyces lilacinus on colonization of polyfoam rooting cubes by rhizoctonia solani.suspensions of pseudomonas cepacia (strain 5.5b) and paecilomyces lilacinus (isolate 6.2f) were applied to polyfoam rooting cubes for control of stem rot of poinsettia caused by rhizoctonia solani. the populations of antagonists and colonization of rooting cubes by r. solani were monitored during a 3-week period. colonization of cubes by r. solani was reduced in cubes treated with p. cepacia, but the population of p. cepacia decreased by as much as 97% during the test period. increased colonizat ...199416349353
oxidative ring cleavage of low chlorinated biphenyl derivatives by fungi leads to the formation of chlorinated lactone derivatives.the yeast trichosporon mucoides and the filamentous fungus paecilomyces lilacinus as biphenyl oxidizing organisms are able to oxidize chlorinated biphenyl derivatives. initial oxidation of derivatives chlorinated at c4 position started at the non-halogenated ring and went on up to ring cleavage. the products formed were mono- and dihydroxylated 4-chlorobiphenyls, muconic acid derivatives 2-hydroxy-4-(4-chlorophenyl)-muconic acid and 2-hydroxy-5-(4-chlorophenyl)-muconic acid as well as the corres ...200616352329
comparison of preparation techniques of mixed samples (fungi-helminth eggs) for scanning electron microscopy by critical point drying.we compared three preparation techniques for critical point drying of fungus paecilomyces lilacinus (thom) samson with toxocara canis (werner) johnston and taenia hydatigena linneo eggs by scanning electron microscopy. we evaluated filtration (first), centrifugation (second), and phytoplankton network (third) in critical point drying methods. the first and third methods were advantageous for t. canis eggs because they preserved the quantity and quality of samples to obtain better images definiti ...200616607559
[reaction of microorganisms to the digestive fluid of the earthworms].the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ...200717410875
multicenter evaluation of a new disk agar diffusion method for susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin b, and caspofungin.the purpose of this study was to correlate inhibition zone diameters, in millimeters (agar diffusion disk method), with the broth dilution mics or minimum effective concentrations (mecs) (clsi m38-a method) of five antifungal agents to identify optimal testing guidelines for disk mold testing. the following disk diffusion testing parameters were evaluated for 555 isolates of the molds absidia corymbifera, aspergillus sp. (five species), alternaria sp., bipolaris spicifera, fusarium sp. (three sp ...200717428932
risk assessment of biocontrol products: a semi-selective medium for improving quantitative isolation of paecilomyces lilacinus (strain 251) from soil.paecilomyces lilacinus (thom) samson 1974 is an egg pathogenic fungus, attacking mainly sedentary stages of root-knot and cyst nematodes, especially eggs, and is about to register as a product for the biological control of nematodes. monitoring p. lilacinus (strain 251) is essential by way of ascertaining the fate and behaviour of this fungus in the soil. in order to obtain more data on the persistence of p. lilacinus (strain 251) in the soil, an improved selective medium for this strain is nece ...200315149104
production of leucinostatins and nematicidal activity of australian isolates of paecilomyces lilacinus (thom) samson.to evaluate the relationship between leucinostatin production by paecilomyces lilacinus isolates and their biological activities.200415214724
topical voriconazole as a novel treatment for fungal keratitis.paecilomyces lilacinus is a fungal pathogen which is generally resistant to amphotericin b and certain other antifungals and is an uncommon cause of devastating fungal keratitis. in the present studies, we evaluated topical voriconazole as therapy for p. lilacinus keratitis in rabbits. thirty eyes of 15 rabbits were studied. in five animals, the uninfected left eye was treated twice daily with voriconazole (drug control, uninfected eye). in these same animals, the right eye was infected with p. ...200616377696
fungal keratitis caused by paecilomyces lilacinus associated with a retained intracorneal hair.to report a case of fungal keratitis caused by paecilomyces lilacinus (p. lilacinus) associated with a retained intracorneal hair.200415220739
a chitosanase from paecilomyces lilacinus with binding affinity for specific chito-oligosaccharides.a purple-spore, rhizosphere-inhabiting nematophagous fungus, further identified as paecilomyces lilacinus, was found to grow on chitosanase-detecting plate. an induced endochitosanase having a molecular mass of 23 kda was purified from the culture medium by a single cation-exchange column-chromatography step. its optimum ph, optimum temperature and pi were found to be 6.0, 50 degrees c and 8.3 respectively. the n-terminal amino acid sequence of the purified enzyme was partially determined. on th ...200515270720
efficacy of paecilomyces lilacinus strain 251 against root knot nematodes in tomato under greenhouse conditions. 200718018889
cloning and phylogenetic analysis of the chitinase gene from the facultative pathogen paecilomyces lilacinus.to pcr-amplify the full-length genomic-encoding sequence for one chitinase from the facultative fungal pathogen paecilomyces lilacinus, analyse the dna and deduced amino acid sequences and compare the amino acid sequence with chitinases reported from mycopathogens, entomopathogens and nematopathogens.200718045433
oxidation and ring cleavage of dibenzofuran by the filamentous fungus paecilomyces lilacinus.the ability of the imperfect soil fungus paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by uv spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectroscopy. biotransformation was initiated by two separate hydroxylation steps, leading to the accumulation of 4-monohydroxylated and 4-dihydroxylateddibenzofurans. hydroxylation at both ...200415278240
development of a bionematicide with paecilomyces lilacinus to control meloidogyne incognita.root-knot disease caused by meloidogyne incognita is a matter of grave concern because it affects several economically important crop plants. the use of solid-state fermentation (ssf) may help to elaborate efficient formulations with fungi to be employed in the biologic control of nematodes. attempts were made to select low-cost substrates for spore production of a strain of paecilomyces lilacinus with known nematicide capacity. coffee husks, cassava bagasse, and defatted soybean cake were utili ...200415304741
hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus glomus claroideum beg 23 is stimulated by humic substances.effects of humic substances (humic acid or fulvic soil extract) or saprophytic microorganisms (paecilomyces lilacinus and an unidentified actinomycete) on growth of mycelium and mycorrhiza formation by glomus claroideum beg23 were studied in a hydroponic system. humic substances stimulated root colonization and production of extraradical mycelium by the mycorrhizal fungus. both humic and fulvic acids tended to decrease populations of culturable bacteria and fungi in the cultivation system, indic ...200515830211
effect of paecilomyces lilacinus on the viability of oncospheres of taenia hydatigena.paecilomyces lilacinus (thom) samson is a saprophytic hyphomycete from the soil with biological activity on helminth eggs. we evaluated the influence in vitro of p. lilacinus on the viability of the oncospheres from taenia hydatigena, a parasite cestode of dogs and sheep. the eggs were exposed to the fungus strain in sterile distilled water and observed by light microscopy at days 4, 7 and 14 post-inoculation, and the viability was evaluated. the viability found in the exposed p. lilacinus oncos ...200515950384
tropical soil microflora of spice-based cropping systems as potential antagonists of root-knot nematodes.suppression of plant parasitic nematodes with nematode predators, parasites or antagonists is an eco-friendly approach than the toxic chemicals. in a study, soil borne fungi from the rhizosphere of major spice crops were collected from diverse cropping systems prevailing in three southern states of india. a series of in vitro studies were conducted using 73 freshly collected fungal isolates and 76 isolates obtained from other sources. out of this 67 isolates were not parasitic on females of root ...200515955340
fungi and actinomycetes associated with meloidogyne spp. eggs and females in china and their biocontrol potential.a survey was conducted to determine the microflora on eggs and females of meloidogyne spp. collected from plant roots and infested soil in china. a total of 455 fungal isolates belonging to 24 genera and 52 isolates of actinomycetes were obtained from 28 samples from greenhouses and fields in hainan, yunnan, fujian, hebei, shandong, and beijing. the predominant fungal species were paecilomyces lilacinus (49.3% of the isolates), fusarium spp. (7.9%), pochonia chlamydosporia (6.9%), penicillium sp ...200616737708
post-traumatic fungal endophthalmitis--a prospective study.to study the incidence, clinical presentation, and the response of anti-fungals in cases of fungal endophthalmitis following open globe injury.200816751752
paecilomyces lilacinus cutaneous infection associated with a dog bite. 200616843134
clinical manifestations, treatment and outcome of paecilomyces lilacinus infections.the fungus paecilomyces lilacinus is an emerging pathogen that causes severe human infections, including devastating oculomycosis. usually, it shows low susceptibility to conventional antifungal drugs in vitro, and variable susceptibility to novel triazoles. a review of the published literature identified 119 reported cases of human infection by p. lilacinus between 1964 and 2004. most were cases of oculomycosis (51.3%), followed by cutaneous and sub-cutaneous infections (35.3%), and a smaller g ...200616961630
fluorescence spectroscopy: a tool to characterize humic substances in soil colonized by microorganisms?the ability of eight soil microfungal species, alternaria alternata, clonostachys rosea f. rosea, exophiala cf. salmonis, fusarium cf. coeruleum, fusarium redolens, paecilomyces lilacinus, penicillium canescens and phoma sp., and two known basidiomycete humic acid (ha) degraders, trametes versicolor and phanerochaete chrysosporium, to modify fluorescence properties of fulvic acids (fa) and/or has was determined. effects of minerals and/or glucose on the modifications were examined. fa purified o ...200617004653
integrated application of some compatible biocontrol agents along with mustard oil seed cake and furadan on meloidogyne incognita infecting tomato plants.experiments were carried out to study the effect of two fungal bioagents along with mustard oil cake and furadan against root knot nematode meloidogyne incognita infecting tomato under greenhouse condition. bioagents viz., paecilomyces lilacinus and trichoderma viride alone or in combination with mustard cake and furadan promoted plant growth, reduced number of galls/plant, egg masses/root system and eggs/egg mass. the fungal bioagents along with mustard cake and nematicide showed least nematode ...200617048300
isolation of paecilomyces lilacinus (thom) samson (ascomycota: hypocreales) from the chagas disease vector, triatoma infestans klug (hemiptera: reduviidae) in an endemic area in argentina.a survey for entomopathogenic fungi of the chagas disease vector triatoma infestans was conducted in two provinces of argentina from march-december 2003. field-collected insects that died in the laboratory were individually maintained in moist chamber and incubated at 22 degrees c. triatominae adults infected with the fungus paecilomyces lilacinus were found at el quebracho (27 degrees 34's-64 degrees 31'w), santiago del estero province, argentina, in december 2003. paecilomyces lilacinus was cu ...200617123036
effects of carbon concentration and carbon to nitrogen ratio on the growth and sporulation of several biocontrol fungi.effects of carbon concentration and carbon to nitrogen (c:n) ratio on six biocontrol fungal strains are reported in this paper. all fungal strains had extensive growth on the media supplemented with 6-12 gl(-1) carbon and c:n ratios from 10:1 to 80:1, and differed in nutrient requirements for sporulation. except for the two strains of paecilomyces lilacinus, all selected fungi attained the highest spore yields at a c:n ratio of 160:1 when the carbon concentration was 12 gl(-1) for metarhizium an ...200717158041
successful treatment of paecilomyces lilacinus keratitis in a patient with a history of herpes simplex virus keratitis.to report a case of paecilomyces lilacinus keratitis, initially misdiagnosed as penicillium sp., in a patient with a long-standing history of herpes simplex virus (hsv) keratitis.200617172908
thiol-dependent serine proteinase from paecilomyces lilacinus: purification and catalytic properties.an extracellular thiol-dependent serine proteinase was isolated from culture medium filtrate of the microscopic fungus paecilomyces lilacinus with a yield of 33%. the enzyme is inactivated by specific inhibitors of serine proteinases, phenylmethylsulfonyl fluoride, as well as by chloromercuribenzoate and mercury acetate, but is resistant to chelating agents. the proteinase has broad specificity, hydrolyzes proteins and p-nitroanilides of n-acylated tripeptides, exhibiting maximal activity in hyd ...200717309445
clinical manifestations, treatment and outcome of paecilomyces lilacinus infections. 200717371539
paecilomyces lilacinus fungemia in a jamaican neonate. 200617373309
posaconazole salvage therapy allows successful allogeneic hematopoietic stem cell transplantation in patients with refractory invasive mold infections.we describe the clinical courses of 3 patients with hematologic malignancies (2 with acute myelogenous leukemia and 1 with multiple myeloma) who developed invasive fungal infections due to uncommon molds (alternaria spp., paecilomyces lilacinus, and zygomycetes). breakthrough invasive fungal infections of the sinus (n=1), lung (n=3), and pericardium (n=1) developed despite fluconazole prophylaxis and failed to respond to treatment with other licensed antifungal therapies, including amphotericin ...200717461992
paecilomyces lilacinus infection in a liver transplant patient: case report and review of the literature.a 56-year-old male who was 12 months status post liver transplant presented with a 2-month history of painful, erythematous nodules over the right knee. several biopsies yielded a mold initially phenotypically identified as a penicillium species, but molecular sequence analysis ultimately determined the identity as paecilomyces lilacinus. several courses of oral voriconazole were required for resolution of the infection. a review of the literature revealed that paecilomyces species are an infreq ...200817605741
cross-reactivity of non-aspergillus fungal species in the aspergillus galactomannan enzyme immunoassay.the aspergillus galactomannan enzyme-linked immunosorbant assay (eia) has been demonstrated to facilitate rapid and sensitive detection of invasive aspergillosis. however, test specificity has not been fully evaluated in non-aspergillus fungal species. of 53 fungal isolates, cross-reactivity was observed with 5 non-aspergillus spp.: blastomyces dermatitidis, nigrospora oryzae, paecilomyces lilacinus, penicillium chrysogenum, and trichothecium roseum.200717662550
[effect of the fungus paecilomyces lilacinus on taenia saginata eggs].with the aim of demonstrating the effectiveness of the fungus paecilomyces lilacinus on taenia saginata eggs under laboratory conditions, a trial was set up in petri dishes with water-agar 2%. there was ovicidal activity (p < 0.05) in relation to the control group on the tenth day of interaction and an internal colonization rate of 25.5% in the eggs.200819142455
paecilomyces lilacinus infection with a sporotrichoid pattern in a renal transplant patient.paecilomyces lilacinus (p. lilacinus) is a saprophytic fungus with increasing prevalence as a source of infection in the immunosuppressed patient population. we report a p. lilacinus infection with a sporotrichoid pattern spreading in a renal transplant recipient that responded to itraconazole therapy. there was no recurrence at 3 years follow-up.200717668542
ovicidal activity of entomopathogenic hyphomycetes on aedes aegypti (diptera: culicidae) under laboratory conditions.the ovicidal activity of 21 hyphomycete fungi species against aedes aegypti (l.) (diptera: culicidae) was tested. fungi with ovicidal activity developed on high numbers of eggs (> or =70%) during 25 d of exposure. a clear ovicidal activity with low values of hatch (1.3-40%) was observed after 25 d of incubation with isaria farinosa (holm: fries) fries, paecilomyces carneus (duché & heim) brown & smith, paecilomyces marquandii (massee) hughes, isaria fumosorosea (wize), metarhizium anisopliae (me ...200717915511
[endophthalmitis due to paecilomyces lilacinus after non-surgical penetrating trauma]. 200111747798
bioremediation of aflatoxins by some reference fungal strains.aspergillus parasiticus rcmb 002001 (2) producing four types of aflatoxins b1, b2, g1, and g2 was used in this study as an aflatoxin-producer. penicillium griseofulvum, p. urticae, paecilomyces lilacinus, trichoderma viride, candida utilis, saccharomyces cerevisiae as well as a non-toxigenic strain of aspergillus flavus were found to be able to exhibit growth on aflatoxin b1-containing medium up to a concentration of 500 ppb. it was also found that several fungal strains exhibited the growth in ...200718062656
paecilomyces lilacinus cutaneous infection associated with peripherally inserted central catheter insertion. 200818331302
response of meloidogyne spp. to pasteuria penetrans, fungi, and cultural practices in tobacco.the response of a mixed population of meloidogyne incognita and m. javanica to three cultural practices, tobacco cultivars (two cultivars, differing in resistance to m. incognita), cover treatments (three treatments), and inorganic nitrogen fertilizer (two treatments), pasteuria penetrans, and soil-borne fungi was investigated in a tobacco field in 1991. on all sampling dates, higher densities of root-knot nematodes were observed on tobacco cv. coker 371 gold than on k-326. initially, forage sor ...199419279937
new azoles as first line therapy for paecilomyces lilacinus in transplant patients. 200818353095
paecilomyces lilacinus olecranon bursitis in an immunocompromised host: case report and review.paecilomyces lilacinus is a little-known mold that causes rare cases of invasive infections in humans regardless of their immune status. we present a unique case in an immunocompromised host with olecranon bursitis because of multidrug-resistant p. lilacinus treated with systemic ketoconazole therapy and surgical debridement. recognition of this fungus is difficult initially because of its appearance, which can be confused with that of other fungi. once this organism has been identified, it is r ...200818358663
liposome-based formulations for the antibiotic nonapeptide leucinostatin a: fourier transform infrared spectroscopy characterization and in vivo toxicologic study.leucinostatin-a is a nonapeptide isolated from paecilomyces marquandii, paecilomyces lilacinus a257, and acremonium sp., exerting remarkable phytotoxic, antibacterial (especially against gram-positive) and antimycotic activities. with the aim to find alternative formulation for in vivo administration, a number of leucinostatin-a-loaded liposomal formulations have been prepared and characterized. both large unilamellar vesicles and multilamellar vesicles consisting of synthetic and natural lipids ...200014727851
screening for indian isolates of egg-parasitic fungi for use in biological control of fascioliasis and amphistomiasis in ruminant livestock.wild isolates of the egg-parasitic fungi paecilomyces lilacinus and verticillium chlamydosporium, obtained from the organic environment of durg, chhattisgarh, india, were subjected to screening for in vitro growth using different media types, range of incubation temperature and ph, and their predatory activity to the eggs of fasciola gigantica and gigantocotyle explanatum. maximum growth of p. lilacinus was obtained in corn-meal agar compared to any other media types. the preferred medium for gr ...200818462555
susceptibility testing and molecular classification of paecilomyces spp.in vitro susceptibility profiles of 58 paecilomyces clinical isolates are reported. amphotericin b, itraconazole, and echinocandins showed poor activity against paecilomyces lilacinus, while the new triazoles were active against it. paecilomyces variotii exhibited a different susceptibility pattern, being susceptible to most antifungal agents apart from voriconazole and ravuconazole.200818519716
white-spot disease of chinese soft-shelled turtles (trionyx sinens) caused by paecilomyces lilacinus.chinese soft-shelled turtles (trionyx sinens) in culture farms using an artificial warming system in zhejiang, china, often show typical signs of white-spot disease such as white spots on their bodies, skin lesions, anorexia and eventually death. the sick turtles were mostly 5~80 g in weight. a suspected fungal pathogen was isolated from the sick turtles and verified as paecilomyces lilacinus by sequence analysis of the internal transcribed spacer (its) of its ribosomal dna (rdna). detailed morp ...200818600789
phylogenetic analysis on the bacteria producing non-volatile fungistatic substances.this study characterized the soil bacteria producing non-volatile fungistatic substances. among the 2,100 colonies of soil bacteria randomly isolated from seven agricultural soil samples, 518 isolates (24.67% of total) showed fungistatic activity toward nematophagous fungi paecilomyces lilacinus and trichoderma viride by producing non-volatile substances. a phylogenetic analysis based on amplified ribosomal dna restriction analysis (ardra) and 16s rdna sequence placed the 518 bacteria in three g ...200818604493
ovicidal activity of paecilomyces lilacinus on moniezia sp. eggs.the ovicidal activity of paecilomyces lilacinus was evaluated on moniezia sp. eggs. eggs of moniezia sp. were incubated on plates with 2% agar-water inoculated with grown fungal isolates and a control treatment without fungus. after 5, 10 and 15 days post-inoculation, the eggs were removed and classified according to the following parameters: effect type 1, lytic effect without morphological damage to eggshells; effect type 2, lytic effect with morphological change in embryos and eggshells; and ...200818613986
bioweathering of chrysotile by fungi isolated in ophiolitic sites.asbestos minerals are commonly found in serpentine rocks and because of the hazard to human health, research has recently focused on possible detoxification strategies. some fungal species that inhabit serpentine sites (two disused chrysotile asbestos mines in the western alps) have been isolated and characterized in order to obtain data on their biodiversity and bioweathering abilities on chrysotile fibres. the three dominant species (verticillium leptobactrum, paecilomyces lilacinus and asperg ...200818616596
fungal endophthalmitis: fourteen years' experience from a centre in india.background:: as fungal endophthalmitis is an emerging challenge, the study was carried out to determine the prevalence and the spectrum of fungal agents causing endophthalmitis from a single center, to identify the risk factors, and to correlate clinical course of illness with the agents involved. methods:: the microbiological and clinical records of all fungal endophthalmitis diagnosed during january 1992 through december 2005 at a tertiary center in india were reviewed retrospectively. during ...200818791488
successful medical treatment of a case of paecilomyces lilacinus keratitis.to report a successful medical treatment of a case of keratitis caused by paecilomyces lilacinus.200818812777
purification and characterization of chitinases from paecilomyces variotii dg-3 parasitizing on meloidogyne incognita eggs.two extracellular chitinases were purified from paecilomyces variotii dg-3, a chitinase producer and a nematode egg-parasitic fungus, to homogeneity by deae sephadex a-50 and sephadex g-100 chromatography. the purified enzymes were a monomer with an apparent molecular mass of 32 kda (chi32) and 46 kda (chi46), respectively, and showed chitinase activity bands with 0.01% glycol chitin as a substrate after sds-page. the first 20 and 15 n-terminal amino acid sequences of chi32 and chi46 were determ ...200918936994
fungal endophthalmitis: fourteen years' experience from a center in india.as fungal endophthalmitis is an emerging challenge, the study was carried out to determine the prevalence and the spectrum of fungal agents causing endophthalmitis from a single center, to identify the risk factors, and to correlate clinical course of illness with the agents involved.200819009680
influence of copper ions on the fatty acid profiles of soil filamentous fungi.the fatty acid profiles have been studied for 5 species 4 genera (9 strains) of soil filamentous fungi isolated from ukrainian soils polluted with heavy metals and reserve territories. palmitic, stearic, oleic and linoleic acids were found in the highest quantities (up to 66.82%) of the total content of fatty acids of fungi. fatty acid unsaturation rate in paecilomyces lilacinus 146 and p. marquandii 153 resistant to copper ions was high (0.85-1.15) under an increase of copper ions concentration ...200819351050
a novel two-stage cultivation method to optimize carbon concentration and carbon-to-nitrogen ratio for sporulation of biocontrol fungi.a two-stage solid cultivation method was used to determine the precise requirements of carbon concentration (1-16 g/l) and c: n ratio (0.625: 1 to 80: 1) for the sporulation of six biocontrol fungi. the c concentration and c: n ratio producing the highest conidia yield were 1 g/l and 5: 1 for paecilomyces lilacinus ipc-p; 2 g/l and 10: 1 for p. lilacinus m-14; 16 g/l and 80: 1 for metarhizium anisopliae sqz-1-21; 4 g/l and 5: 1 for m. anisopliae rs-4-1 and lecanicillium lecanii ca-1-g; and 2 g/l ...200919418252
paecilomyces lilacinus peritonitis complicating peritoneal dialysis cured by oral voriconazole and terbinafine combination therapy.fungal peritonitis (fp) is a serious complication in patients on continuous ambulatory peritoneal dialysis (capd). we report a case of capd-related fp caused by paecilomyces lilacinus in a 15-year-old uraemic boy. the infection was successfully treated by combination therapy consisting of oral voriconazole and terbinafine, which has not been previously reported in the treatment of fp.200819018033
posaconazole efficacy in a murine disseminated infection caused by paecilomyces lilacinus.we have compared the efficacy of posaconazole and amphotericin b in an experimental murine model of paecilomycosis.200919054804
subaortic aneurysm caused by paecilomyces lilacinus endocarditis. 200819059579
in vitro activities of isavuconazole against opportunistic filamentous and dimorphic fungi.the in vitro activity of isavuconazole was compared to those of amphotericin b, fluconazole, itraconazole, voriconazole, posaconazole, and ravuconazole against 300 clinical isolates of pseudallescheria boydii, paecilomyces lilacinus, fusarium spp., bipolaris spicifera, curvularia lunata, alternaria alternata, exophiala spp., rhizopus arrhizus, mucor circillenoides, absidia corymbifera, blastomyces dermatitidis, histoplasma capsulatum and coccidioides posadasii. mics were determined by a broth ma ...200919101837
pathogenesis and outcome of paecilomyces keratitis.to examine the clinical pathology and management of paecilomyces lilacinus keratitis.200919195638
preliminary crystallographic study of two cuticle-degrading proteases from the nematophagous fungi lecanicillium psalliotae and paecilomyces lilacinus.cuticle-degrading proteases are extracellular subtilisin-like serine proteases that are secreted by entomopathogenic and nematophagous fungi. these proteases can digest the host cuticle during invasion of an insect or nematode and serve as a group of important virulence factors during the infection of nematodes by nematophagous fungi. to elucidate the mechanism of interaction between the proteases and the nematode cuticle, two cuticle-degrading proteases, ver112 from lecanicillium psalliotae (sy ...200919255481
viability of heterodera glycines exposed to fungal filtrates.filtrates from nematode-parasitic fungi have been reported to be toxic to plant-parasitic nematodes. our objective was to determine the effects of fungal filtrates on second-stage juveniles and eggs of heterodera glycines. eleven fungal species that were isolated from cysts extracted from a soybean field in florida were tested on j2, and five species were tested on eggs in vitro. each fungal species was grown in czapek-dox broth and malt extract broth. no toxic activity was observed for fungi gr ...200019270965
pathogenicity of fungi to eggs of heterodera glycines.twenty-one isolates of 18 fungal species were tested on water agar for their pathogenicity to eggs of heterodera glycines. an egg-parasitic index (epi) for each of these fungi was recorded on a scale from 0 to 10, and hatch of nematode eggs was determined after exposure to the fungi on water agar for 3 weeks at 24 c. the epi for verticillium chlamydosporium was 7.6, and the fungus reduced hatch 74%. pyrenochaeta terrestris and two sterile fungi also showed a high epi and reduced hatch 42-73%. ar ...199619277130
efficacy of paecilomyces lilacinus in suppressing rotylenchulus reniformis on tomato.effects of rice-cultured paecilomyces lilacinus on rotylenchulus reniformis were studied in both greenhouse and field microplot tests with 'rutgers' tomato. numbers of r. reniformis were reduced (p </= 0.05) by p. lilacinus, with suppression in the initial greenhouse test ranging from 46 to 48% for two rice + p. lilacinus treatments; the rice-only treatment caused a nonsignificant reduction of 25%. in the second greenhouse test, total r. reniformis numbers were restricted (p </= 0.05) by 41% by ...199419279933
successful treatment with voriconazole of prolonged paecilomyces lilacinus fungemia in a chronic hemodialyzed patient.paecilomyces lilacinus is a saprophytic mould which rarely causes infection in humans. we report a case of paecilomyces lilacinus catheter-related fungemia in a chronic hemodialyzed patient. blood cultures remained positive for 8 weeks. the infection was cured after eventual acceptance by the patient of oral voriconazole treatment for 6 weeks and removal of the tunneled catheter. the literature on paecilomyces fungemia in humans is reviewed.200919281753
management of root knot nematode on tomato through application of fungal antagonists, acremonium strictum and trichoderma harzianum.management of root knot nematode disease infecting tomato, by the use of fungal bioagents acremonium strictum and trichoderma harzianum isolated from egg masses of m. incognita infecting tomato has been carried out. the rhizosphere and rhizoplane of root knot nematode infested tomato revealed consistent association of acremonium strictum. in the present study a. strictum and other fungal bioagents viz. aspergillus niger, paecilomyces lilacinus, rhizoctonia solani and trichoderma harzianum isolat ...200818368544
toluene gas phase biofiltration by paecilomyces lilacinus and isolation and identification of a hydrophobin protein produced thereof.paecilomyces lilacinus consumed toluene as the sole carbon source in a gas-phase biofilter packed with perlite obtaining an average elimination capacity of 50 g m(-3) h(-1), a removal efficiency of 53%, and a final biomass of 31.6 mg biomass g dry support(-1). hydrophobin proteins from the mycelium produced in the biofilter were purified by formic acid extraction and precipitated by electrobubbling, and the molecular weight was found to be 10.6 +/- 0.3 kda. the peptide mass fingerprinting analys ...200818443779
growth of isolates of paecilomyces lilacinus and their efficacy in biocontrol of meloidogyne incognita on tomato.the potential of 13 paecilomyces lilacinus isolates from various geographic regions as biocontrol agents against meloidogyne incognita, the effects of temperature on their growth, and the characterization of the impact of soil temperature on their efficacy for controlling this nematode were investigated. maximum fungal growth, as determined by dry weight of the mycelium, occurred from 24 to 30 c; least growth was at 12 and 36 c. the best control of m. incognita was provided by an isolate from pe ...198919287594
meloidogyne incognita survival in soil infested with paecilomyces lilacinus and verticillium chlamydosporium.meloidogyne incognita-infected tomato seedlings were transplanted into sterilized soil or unsterilized soil collected from 20 california tomato fields to measure suppression caused by paecilomyces lilacinus, verticillium chlamydosporium, and other naturally occurring antagonists. unsterilized soils q, a, and h contained 35, 39, and 55% fewer m. incognita second-stage juveniles (j2) than did sterilized soil 1 month after infected tomato seedlings were transplanted to these soils and placed in a g ...199019287707
association of verticillium chlamydosporium and paecilomyces lilacinus with root-knot nematode infested soil.population densities of meloidogyne incognita and the nematophagous fungi, paecilomyces lilacinus and verticillium chlamydosporium, were determined in 20 northern california tomato fields over two growing seasons. paecilomyces lilacinus was isolated from three fields, v. chlamydosporium was isolated from one field, and both fungi were isolated from 12 fields. verticillium chlamydosporium numbers were positively correlated with numbers of m. incognita and p. lilacinus. paecilomyces lilacinus numb ...199019287711
antagonists of plant-parasitic nematodes in florida citrus.in a survey of antagonists of nematodes in 27 citrus groves, each with a history of tylenchulus semipenetrans infestation, and 17 noncitrus habitats in florida, approximately 24 species of microbial antagonists capable of attacking vermiform stages of radopholus citrophilus were recovered. eleven of these microbes and a species of pasteuria also were observed attacking vermiform stages of t. semipenetrans. verticillium chlamydosporium, paecilomyces lilacinus, p. marquandii, streptomyces sp., art ...199019287759
pathogenicity of some hypocrealean fungi to adult aedes aegypti (diptera: culicidae).the pathogenicity of 19 hypocrealean entomopathogenic fungi from seven different genera in adult aedes aegypti was tested. all fungi proved to be pathogenic, and isaria fumosorosea, lecanicillium muscarium, lecanicillium psalliotae, metarhizium anisopliae, metarhizium lepidiotae, metarhizium majus, metarhizium frigidum, paecilomyces carneus, and paecilomyces lilacinus caused total mortality within 15 days of exposure of mosquitoes to the fungal culture. all fungi developed on dead individuals. t ...201020680340
biological control of meloidogyne incognita by paecilomyces lilacinus and pasteuria penetrans.the root-knot nematode meloidogyne incognita was controlled more effectively and yields of host plants were greater when paecilomyces lilacinus and pasteuria penetrans were applied together in field microplots than when either was applied alone. yields of winter vetch from microplots inoculated with the nematode and with both organisms were not statistically different from yields from uninoculated control plots.198719290133
a semi-selective medium for the isolation of paecilomyces lilacinus from soil. 198719290138
histology of the interactions of paecilomyces lilacinus with meloidogyne incognita on tomato.excised tomato roots were examined histologically for interactions of the fungus paecilomyces lilacinus and meloidogyne incognita race 1. root galling and giant-cell formation were absent in tomato roots inoculated with nematode eggs infected with p. lilacinus. few to no galls and no giant-cell formation were found in roots dipped in a spore suspension of p. lilacinus and inoculated with m. incognita. numerous large galls and giant cells were present in roots inoculated only with m. incognita. p ...198819290224
evaluation of paecilomyces lilacinus as a biocontrol agent of meloidogyne javanica on tobacco.the efficacy of the nematode parasite paecilomyces lilacinus, alone and in combination with phenamiphos and ethoprop, for controlling the root-knot nematode meloidogyne javanica on tobacco and the ability of this fungus to colonize in soil under field conditions were evaluated for 2 years in microplots. combinations and individual treatments of the fungus grown on autoclaved wheat seed, m. javanica eggs (76,000 per plot), and nematicides were applied to specified microplots at the time of transp ...198819290257
fungal parasites of the cereal cyst nematode heterodera avenae in southern sweden.fungal parasites of the cereal cyst nematode heterodera avenae were isolated from four sites in southern sweden. in all, 15 different fungi were isolated from different stages of the nematode life cycle. among the egg parasites, verticillium chlamydosporium was common in young cysts on roots, whereas an unidentified species of verticillium (verticillium sp. 1) was the dominating species in cysts from soil, especially if the soll had been stored for 8-12 months. v. chlamydosporium was frequently ...198519294057
biocontrol of meloidogyne incognita on tomato using antagonistic fungi, plant-growth-promoting rhizobacteria and cattle manure.biocontrol achieved by a single biocontrol agent is generally inconsistent under field conditions. the aim of the present study was to increase the competitiveness and efficacy of biocontrol agents by using them together with cattle manure.200919431151
[antagonistic interactions between saprotrophic fungi and geohelminths. 1. saprotrophic fungi in the biological control of phytopathogenic geohelminths].the state of knowledge on the possible antagonism between soil saprotrophic fungi and phytopathogenic nematodes of the genera meloidogyne, heterodera, and globodera is reviewed basing on the literature and our own research. mycelial colonisation of various developmental stages of these geohelminths is the most common factor thought to reduce their populations in nature. the following parasitic fungi can be found on the cysts, eggs, as well as the larvae of the nematodes: paecilomyces lilacinus, ...200919579778
paecilomyces lilacinus eumycetoma.eumycetoma is a chronic granulomatous infection of the skin, subcutaneous tissue, fascia, and bone caused by true fungi. most commonly, it affects the foot or hand. fungi commonly reported to cause eumycetoma are madurella mycetomatis, madurella grisea, phialophora jeanselmei, cephalosporium recifei, etc. there have been several previous reports of human invasive infections by paecilomyces lilacinus causing endophthalmitis, keratitis, chronic sinusitis, skin and soft tissue infections, and cathe ...200919659864
fungus-benzimidazole interactions: a prerequisite to deploying egg-parasitic fungi paecilomyces lilacinus and verticillium chlamydosporium as biocontrol agents against fascioliasis and amphistomiasis in ruminant livestock.in vitro trials investigating the effects of albendazole and triclabendazole anthelmintics on the growth profiles of the egg-parasitic fungi paecilomyces lilacinus and verticillium chlamydosporium were undertaken. in addition, in vivo trials were conducted in goats fed on millet grain cultures of each fungus and administered albendazole and triclabendazole anthelmintics. in vitro growth revealed v. chlamydosporium to be more sensitive to albendazole compared to p. lilacinus. in contrast, triclab ...201019698195
hyalohyphomycosis caused by paecilomyces lilacinus after kidney transplantation.hyalohyphomycosis caused by paecilomyces has rarely been described among solid organ recipients. its management is elusive without an established consensus concerning antifungal therapy. herein we have reported a case of extensive cellulitis caused by paecilomyces lilacinus observed in a 48-year-old kidney transplanted woman with hepatitis c. kidney transplantation from a cadaveric donor was performed in october 2006 with an uneventful early course except for posttransplant diabetes mellitus and ...200919765473
[a comparative investigation of adaptation to oxidative stress factors a strain of mycelial fungus paecilomyces lilacinus from chernobyl atomic energy station and strains of the same species from territories with basic level of radioactive pollution].an oxidative stress resistance in paecilomyces lilacinus strain from chernobyl atomic energy station (chaes) as well as in p. lilacinus strains from zones with control level of radioactive pollution has been studied. it was shown that radial growth rate of the colony of the chaes strain in a range of glucose concentrations 0.002%; 0.2%; 0.5%; 1.0% showed maximum on the medium with 0.2% glucose. it was 1.5 times higher than growth rate of the control strains. adaptation slowdown reaction has been ...200919799363
[effect of heavy metal ions on the soil mycobiota in kryvyÄ­ rih region].ecological-taxonomic analysis of mycobiota from heavy metal polluted soils in kryvyi rig town and its residential suburbs are presented in this study the mycobiota consists of45 filamentous species (127 strains) belonging to 28 genera from 3 divisions. among them myrothecium leucotrichum was described as a new species for ukrainian mycobiota while absidia cylindrospora and gongronella butleri as rare species. paecilomyces lilacinus and p. marquandii dominated in the soils near the factory kryvor ...200919938617
ovicidal activity of pochonia chlamydosporia and paecilomyces lilacinus on toxocara canis eggs.an assessment was made of the ovicidal activity of egg-parasitizing fungi pochonia chlamydosporia (isolates vc1 and vc4) and paecilomyces lilacinus on toxocara canis eggs in vitro. the fungal isolates were inoculated onto petri dishes with 2% water-agar (2% wa) and stored at 25 degrees c for 10 days in an incubator, in the dark. the control group was comprised of petri dishes without fungi, containing the 2%wa medium only. later, 1000 embryonated eggs were placed on the surface of the plates wit ...201020097478
the successful medical treatment of a case of paecilomyces lilacinus keratitis.to report the successful medical treatment of paecilomyces lilacinus keratitis case with topical voriconazole.201020098311
the use of voriconazole in the management of post-penetrating keratoplasty paecilomyces keratitis.the aim of this study was to report successful treatment of paecilomyces lilacinus keratitis with voriconazole.200919284327
impact of paecilomyces lilacinus inoculum level and application time on control of meloidogyne incognita on tomato.microplot experiments were conducted to evaluate the effects of inoculum level and time of application of paecilomyces lilacinus on the protection of tomato against meioidogyne incognita. the best protection against m. incognita was attained with 10 and 20 g of fungus-infested wheat kernels per microplot which resulted in a threefold and fourfold increase in tomato yield, respectively, compared with tomato plants treated with this nematode alone. greatest protection against this pathogen was att ...198919287585
survival of paecilomyces lilacinus in selected carriers and related effects on meloidogyne incognita on tomato.laboratory and microplot experiments were conducted to determine the influence of carrier and storage of paecilomyces lilacinus on its survival and related protection of tomato against meloidogyne incognita. spores of p. lilacinus were prepared in five formulations: alginate pellets (pellets), diatomaceous earth granules (granules), wheat grain, soil, and soil plus chitin. fungal viability was high in wheat and granules, intermediate in pellets, and low in soil and chitin-amended soil stored at ...198919287586
identification of paecilomyces variotii in clinical samples and settings.paecilomyces variotii is a commonly occurring species in air and food, but it is also associated with many types of human infections and is among the emerging causative agents of opportunistic mycoses in immunocompromised hosts. paecilomyces can cause hyalohyphomycosis, and two species, paecilomyces lilacinus and p. variotii, are the most frequently encountered organisms. in the present study, a set of 34 clinical isolates morphologically identified as p. variotii or p. lilacinus were formally i ...201020519470
improvement of phytoextraction and antioxidative defense in solanum nigrum l. under cadmium stress by application of cadmium-resistant strain and citric acid.remediation of plant-microorganism-chelates synergy has been proposed as an effective remediation method for enhancing the removal efficiency of heavy metal. manipulation of the antioxidative system increases plant tolerance, thereby potentially enhancing the uptake capacity to heavy metal. in this study, we investigated the possibility of improving the phytoextraction of cd and the antioxidative defense of solanum nigrum l. by application of a new isolated strain (paecilomyces lilacinus nh1) (p ...201020566243
paecilomyces lilacinus in transplant patients: an emerging infection. 201020610372
[transformation of enhanced green fluorescent protein gene in paecilomyces lilacinus mediated by agrobacterium tumefaciens].the main aim of this study was to transform the enhanced green fluorescent protein gene (egfp) into biocontrol fungus paecilomyces lilacinus strain 9410. we constructed the expression vector pupngt of the fusion gene nptii-egfp using pcdna3.1(-) as a helper plasmid. the egfp gene was then transformed into p. lilacinus strain 9410 via agrobacterium tumefaciens-mediated transformation. pcr and southern blotting analysis showed that the egfp gene was integrated into the genomes of the tested transf ...201020684307
effect of meloidogyne incognita inoculum density and application rate of paecilomyces lilacinus strain 251 on biocontrol efficacy and colonization of egg masses analyzed by real-time quantitative pcr.the fungal biocontrol agent, paecilomyces lilacinus strain 251 (pl251), was evaluated for its potential to control the root-knot nematode meloidogyne incognita on tomato at varying application rates and inoculum densities. conversely to previous studies, significant dose-response relationships could not be established. however, we demonstrated that a preplanting soil treatment with the lowest dose of commercially formulated pl251 (2 × 10(5) cfu/g soil) was already sufficient to reduce root galli ...201120822430
in-vitro study of the host-parasite interactions between mouse macrophages and the opportunistic fungus paecilomyces lilacinus. 201020863442
[isolation, purification and structure analysis of polysaccharides from peacilomyces lilacinus].the paper dealt with the characterization of polysaccharide of paecilomyces lilacinus nh-pl-03 strain. first, we extracted and purified exude polysaccharide from the fungal fermentation broth by ethanol depositing method. second, the proteins were removed by the sevage method from the crude polysaccharide. third, the purified polysaccharide (ep-1) was obtained after superdex g-75 column separation. the results of uv-spectrometer and sephacryl s-200 hr chromatography experiments showed that the e ...201021090112
overexpression of a cuticle-degrading protease ver112 increases the nematicidal activity of paecilomyces lilacinus.due to their ability to degrade the proteins in nematode cuticle, serine proteases play an important role in the pathogenicity of nematophagous fungi against nematodes. the serine protease ver112 was identified from the nematophagous fungus lecanicillium psalliotae capable of degrading the nematode cuticle and killing nematodes effectively. in this study, the gene ver112 was introduced into the commercial biocontrol fungal agent paecilomyces lilacinus by the restriction enzyme-mediated integrati ...201021110018
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