| serotonin (5-ht) enhances the activity of amphotericin b against aspergillus fumigatus in vitro. | we investigated the in vitro synergistic antifungal potential of combining serotonin (5-ht) and sertraline with amphotericin b and itraconazole against clinical isolates of aspergillus spp. synergy tests were performed using the chequerboard microdilution method. activity was measured against aspergillus fumigatus (n = 7), aspergillus flavus (n = 3) and aspergillus terreus (n = 2), and compared with that for candida albicans and candida parapsilosis. the fractional inhibitory concentration (fic) ... | 2004 | 15380269 |
| antifungal therapy of murine aspergillus terreus infection. | aspergillus terreus is a species which is being seen increasingly frequently and which is highly resistant to amphotericin b in vitro and clinically. we evaluated amphotericin b, caspofungin, and posaconazole in a murine model of acute invasive aspergillosis. caspofungin and posaconazole both appeared beneficial and may be reasonable treatment alternatives for infection with a. terreus. | 2004 | 15388425 |
| fractionation of the naringinase complex from aspergillus terreus by dye affinity chromatography. | affinity chromatography with immobilised triazine dyes was used to separate the main enzymes present in the naringinase complex produced by aspergillus terreus cect 2663. one alpha-l-rhamnosidase and two beta-d-glucosidases (beta g1 and beta g2) were separated by a simple two-step procedure involving chromatography with red he-3b immobilised on sepharose 4b first at ph 5.5 and then at ph 4.7. maximum activity of the beta-d-glucosidases was from ph 4 to 6 and at 65 degrees c. both glucosidases we ... | 2004 | 15483384 |
| production of l-asparaginase by filamentous fungi. | l-asparaginase production was investigated in the filamentous fungi aspergillus tamarii and aspergillus terreus. the fungi were cultivated in medium containing different nitrogen sources. a. terreus showed the highest l-asparaginase (activity) production level (58 u/l) when cultivated in a 2% proline medium. both fungi presented the lowest level of l-asparaginase production in the presence of glutamine and urea as nitrogen sources. these results suggest that l-asparaginase production by of filam ... | 2004 | 15543411 |
| detection of aflatoxin-producing molds in korean fermented foods and grains by multiplex pcr. | an assay based on multiplex pcr was applied for the detection of potential aflatoxin-producing molds in korean fermented foods and grains. three genes, avfa, omta, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex pcr. dna extracted from aspergillus flavus, aspergillus parasiticus, aspergillus oryzae, aspergillus niger, aspergillus terreus, penicillium expansum, and fusarium verticillioides was used as pcr template to test spe ... | 2004 | 15553652 |
| invasive fungal infections in kuwait: a retrospective study. | a retrospective review of a five year period (1994-1998) revealed that opportunistic mycoses caused by ubiquitous fungal pathogens are a serious problem in the immunocompromised patient population of kuwait. patients with renal transplantation and diabetes mellitus were most susceptible to aspergillosis, cryptococcosis, and zygomycosis, whereas patients with candidemia/hematogenous candidiasis had multiple risk factors. basidiobolomycosis of the rectum in a bangladeshi male, cryptococcosis due t ... | 2000 | 15597675 |
| isoterreulactone a, a novel meroterpenoid with anti-acetylcholinesterase activity produced by aspergillus terreus. | a new seven-membered lactone type meroterpenoid, isoterreulactone a, was isolated from the solid state fermentation of aspergillus terreus and its structure was established by various spectral analysis. isoterreulactone a inhibited acetylcholinesterase with an ic(50) value of 2.5 microm while did not inhibit butyrylcholinesterase even at 500 microm. | 2005 | 15603953 |
| efficacy of caspofungin against aspergillus terreus. | we investigated the in vitro and in vivo activities of caspofungin against aspergillus terreus. the drug increased survival and reduced tissue fungal burden in neutropenic mice. therefore, our data support the role of caspofungin in treating systemic infections due to this emerging pathogen. | 2005 | 16304185 |
| total structural assignment and absolute configuration of terreinol by 13c and 1h nmr. | the carbon-carbon connectivity of terreinol, a new metabolite isolated from aspergillus terreus, and its previous (13)c assignments were confirmed by a two-dimensional inadequate experiment using a few milligrams of the compound with natural (13)c abundance. the carbon-carbon correlations were determined by computational analysis (with >99% probability) of this experiment. additionally, the absolute configuration of terreinol was achieved indirectly via its corresponding secondary alcohol by the ... | 2005 | 15625725 |
| synthesis and biological evaluation of analogues of butyrolactone i and molecular model of its interaction with cdk2. | a series of analogues of butyrolactone i, a natural product isolated from aspergillus terreus that selectively inhibits the cdk2 and cdk1 kinases and that has been found to exhibit an interesting antiproliferative activity, have been synthesized. its antitumor activity has been tested. molecular models of the complex between butyrolactone i and the cdk2 active site have been built using a combination of conformational search and automated docking techniques. the stability of the resulting comple ... | 2004 | 15227538 |
| pellet morphology, culture rheology and lovastatin production in cultures of aspergillus terreus. | pellet growth of aspergillus terreus atcc 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pellet morphology, broth rheology and lovastatin production. the agitation speed and aeration methods used did not affect the biomass production profiles, but significantly influenced ... | 2004 | 15652430 |
| [farmer's lung cases of a farmer and his son with high bal fluid beta-d glucan levels]. | a farmer and his son, who treated straw in a cowshed, were admitted to our hospital because of severe dyspnea during summer time. their chest x-ray films revealed bilateral reticulonodular shadows in the middle to lower lung fields. bronchoalveolar lavage (bal) fluid analyses showed a high proportion of lymphocytes and an increased cd4/8 ratio. they were diagnosed with farmer's lung and treated with pulse therapy with methylprednisolone and tapering of steroid. hypoxemia and interstitial shadow ... | 2004 | 15678910 |
| intrinsic in vitro susceptibility of primary clinical isolates of aspergillus fumigatus, aspergillus terreus, aspergillus nidulans, candida albicans and candida lusitaniae against amphotericin b. | a total of 60 clinical fungal isolates from patients without prior amphotericin b treatment and three control strains were evaluated for their intrinsic susceptibility to amphotericin b (amb) using microdilution, etest and disc diffusion assays, on three media each, roswell park memorial institute (rpmi) 1640, antibiotic medium 3 (am3) and high resolution medium. the fungal strains included isolates of aspergillus fumigatus (n = 10), aspergillus terreus (n = 12), aspergillus nidulans (n = 9), ca ... | 2006 | 16466441 |
| disseminated aspergillosis in two dogs in israel. | aspergillus terreus, normally a soil or plant saprophyte, causes disseminated systemic infection, involving primarily the skeletal and the cardiopulmonary system in humans and dogs.(1, 2) we describe two cases of german shepherd dogs that were referred to koret school of veterinary medicine teaching hospital with a history of anorexia and weakness. case 1 suffered from neurological deficits, paraparesis and lumbar pain whereas case 2 suffered from unilateral uveitis and exophthalmus. both dogs w ... | 2006 | 16466447 |
| 4-hydroxykigelin and 6-demethylkigelin, root growth promoters, produced by aspergillus terreus. | root growth promoters, 4-hydroxykigelin (1) and 6-demethylkigelin (2), together with 6-hydroxymellein (3) were isolated from cultures of the fungus aspergillus terreus and their structures were identified by spectroscopic analysis. the biological activities of the three dihydroisocoumarins, 1, 2, and 3, have been examined using a bioassay method with lettuce seedlings. furthermore, interactions between the dihydroisocoumarins and indole-3-acetic acid against the root growth have been examined. | 2004 | 15241930 |
| in vitro analyses, animal models, and 60 clinical cases of invasive aspergillus terreus infection. | | 2004 | 15328076 |
| antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind. | plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ... | 2006 | 16600543 |
| studies in the biochemistry of micro-organisms: the metabolic products of aspergillus terreus thom. a new mould metabolic product-terrein. | | 1935 | 16745705 |
| novel method for cell immobilization and its application for production of organic acid. | the aim was to develop a novel and simple technique for the entrapment of fungal hyphae. | 2005 | 15715641 |
| studies in the biochemistry of micro-organisms: the molecular constitution of terrein, a metabolic product of aspergillus terreus thom. | | 1937 | 16746426 |
| studies in the biochemistry of micro-organisms: the molecular constitution of geodin and erdin, two chlorine-containing metabolic products of aspergillus terreus thom. part i. the constitutional relationship of geodin and erdin. | | 1937 | 16746438 |
| studies in the biochemistry of micro-organisms: the molecular constitution of geodin and erdin, two chlorine-containing metabolic products of aspergillus terreus thom. part ii. dihydrogeodin and dihydroerdin and the synthesis of their trimethyl ethers. | | 1939 | 16746946 |
| studies in the biochemistry of micro-organisms: itaconic acid, a metabolic product of a strain of aspergillus terreus thom. | | 1939 | 16747058 |
| studies on the biochemistry of micro-organisms: 74. the molecular constitution of geodin and erdin, two chlorine-containing metabolic products of aspergillus terreus thom. part 3. possible structural formulae for geodin and erdin. | | 1947 | 16748191 |
| stimulation of alkalothermophilic aspergillus terreus xylanase by low-intensity laser radiation. | in this study, aspergillus terreus was irradiated by a 7.3 mw he-ne laser in the presence of crystal violet, toluidine blue o and hematoporphyrin as photosensitizers. xylanases recovered from non-irradiated and irradiated fungi were purified and characterized. the maximum production of xylanase (42.2 u/ml) was obtained after 5 min of laser irradiation in the absence of the photosensitizer. the irradiation of the sensitized fungus diminished the production of xylanase. on purification using g-100 ... | 2006 | 16810525 |
| the anticancer activity of the fungal metabolite terrecyclic acid a is associated with modulation of multiple cellular stress response pathways. | tumors are dependent on cellular stress responses, in particular the heat shock response, for survival in their hypoxic, acidotic, and nutrient-deprived microenvironments. using cell-based reporter assays, we have identified terrecyclic acid a (tca) from aspergillus terreus, a fungus inhabiting the rhizosphere of opuntia versicolor of the sonoran desert, as a small-molecule inducer of the heat shock response that shows anticancer activity. further characterization suggested that tca also affects ... | 2005 | 16227407 |
| comparison of the eucast-afst broth dilution method with the clsi reference broth dilution method (m38-a) for susceptibility testing of posaconazole and voriconazole against aspergillus spp. | the susceptibilities of 40 clinical isolates of aspergillus spp. (aspergillus fumigatus, aspergillus flavus, aspergillus niger, aspergillus terreus) were determined for posaconazole and voriconazole by the clsi m38-a and eucast-afst broth dilution methods. where a discrepancy was observed between the methods, the eucast method tended to give higher mic values. overall, the level of agreement was 92.5% and the intra-class correlation coefficient was > 0.90. | 2006 | 16882296 |
| subcellular localization of fructosyl amino acid oxidases in peroxisomes of aspergillus terreus and penicillium janthinellum. | fructosyl amino acid oxidase (faod) is the enzyme catalyzing the oxidative deglycation of amadori compounds, such as fructosyl amino acids, yielding the corresponding amino acids, glucosone, and h(2)o(2). in a previous report, we determined the primary structures of cdnas coding for faods from two fungal strains aspergillus terreus ap1 and penicillium janthinellum and we found that both fungal faods included the putative peroxisome targeting signal 1 (pts1) at the carboxyl terminal (yoshida, n. ... | 1999 | 16232435 |
| metal complexes of schiff base derived from sulphametrole and o-vanilin. synthesis, spectral, thermal characterization and biological activity. | metal complexes of schiff base derived from condensation of o-vanilin (3-methoxysalicylaldehyde) and sulfametrole [n(1)-(4-methoxy-1,2,5-thiadiazole-3-yl)sulfanilamide] (h2l) are reported and characterized based on elemental analyses, ir, 1h nmr, solid reflectance, magnetic moment, molar conductance, mass spectra, uv-vis and thermal analysis (tga). from the elemental analyses data, the complexes were proposed to have the general formulae [m2x3(hl)(h2o)5].yh2o (where m=mn(ii), co(ii), ni(ii), cu( ... | 2007 | 16891151 |
| purification and characterization of an alkaline manganese peroxidase from aspergillus terreus ld-1. | we report that aspergillus terreus ld-1 produces an extracellular ligninolytic enzyme, manganese peroxidase (mnp), that reacts under alkaline conditions. this mnp was purified 13.1-fold from the culture supernatant to elicit a single band upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the molecular weight of this mnp was estimated as either 43 kda by sds-page or 44 kda by gel permeation chromatography, suggesting a monomeric structure. the optimum ph and temperature o ... | 2002 | 16233222 |
| purification and characterization of cis-aconitic acid decarboxylase from aspergillus terreus tn484-m1. | cis-aconitic acid decarboxylase (cad) was assumed to be a key enzyme in the production of itaconic acid by comparing the activity of cad from aspergillus terreus tn484-m1 with that of cad from the low-itaconate yielding strain aspergillus terreus cm85j. the constitutive cad was purified to homogeneity from a. terreus tn484-m1 by ammonium sulfate fractionation, and column chromatography on deae-toyopearl, butyl-toyopearl, and sephacryl s200hr, and then characterized. a molecular mass of 55 kda fo ... | 2002 | 16233265 |
| antifungal efficacy and pharmacodynamics of posaconazole in experimental models of invasive fungal infections. | posaconazole is a novel lipophilic antifungal triazole with potent and broad-spectrum activity against opportunistic, endemic and dermatophytic fungi. this activity extends to organisms that are often refractory to existing triazoles, amphotericin b or echinocandins such as candida glabrata, candida krusei, aspergillus terreus, fusarium spp. and the zygomycetes. a large number of experimental animal models of invasive fungal infections has demonstrated the potent and broad-spectrum efficacy of p ... | 2006 | 16961576 |
| analysis of subunit interactions in the iterative type i polyketide synthase atx from aspergillus terreus. | | 2006 | 17004275 |
| efficacy of caspofungin against aspergillus flavus, aspergillus terreus, and aspergillus nidulans. | the echinocandin caspofungin is a potent inhibitor of the activity of 1,3-beta-d-glucan synthase from aspergillus flavus, aspergillus terreus, and aspergillus nidulans. in murine models of disseminated infection, caspofungin prolonged survival and reduced the kidney fungal burden. caspofungin was at least as effective as amphotericin b against these filamentous fungi in vivo. | 2006 | 17015628 |
| application of oxygen vectors to aspergillus terreus cultivation. | research was undertaken to examine the effects of adding an oxygen carrier (n-dodecane, n-tetradecane, or n-hexadecane) on lovastatin production by aspergillus terreus in both shaking-flask and 5-l fermentor cultivations. in the shaking-flask cultivation, an addition of 2.5% (w/v) n-dodecane to the medium gave about a 1.4-fold increase (0.51 g/l) in lovastatin production. this improvement was related to morphological changes in the fungal cells, and resulted mainly from the formation of small, u ... | 2002 | 16233333 |
| cloning, expression, and characterization of a xylanase 10 from aspergillus terreus (bcc129) in pichia pastoris. | a full-length xylanase gene, encoding 326 amino acids belonging to the fungal glycosyl hydrolase family 10, from aspergillus terreus bcc129 was cloned and sequenced. sequence analysis suggested that the first 25 amino acids of this enzyme is the signal peptide. therefore, only the mature xylanase gene of 906 bp was cloned into a yeast expression vector, ppiczalphaa, for heterologous expression in pichia pastoris. a band of approximately, 33 kda was observed on the sds-page gel after one day of m ... | 2006 | 16275128 |
| purification and properties of beta-glucosidase from aspergillus terreus. | a beta-glucosidase (ec 3.2.1.21) from the fungus aspergillus terreus was purified to homogeneity as indicated by disc acrylamide gel electrophoresis. optimal activity was observed at ph 4.8 and 50 degrees c. the beta-glucosidase had k(m) values of 0.78 and 0.40 mm for p-nitrophenyl-beta-d-glucopyranoside and cellobiose, respectively. glucose was a competitive inhibitor, with a k(i) of 3.5 mm when p-nitrophenyl-beta-d-glucopyranoside was used as the substrate. the specific activity of the enzyme ... | 1982 | 16346147 |
| use of high inoculum for early metabolic signalling and rapid susceptibility testing of aspergillus species. | to develop and evaluate a new method for rapid susceptibility testing of aspergillus spp. based on early metabolic signalling of high-inoculum biomass. | 2007 | 17185296 |
| biosynthesis of territrems by aspergillus terreus. | different radioactive precursors were added to 8-day potato-dextrose liquid cultures of aspergillus terreus 23-1. territrems were isolated from chloroform extracts of the cultures at day 14 and purified by thin-layer chromatography and high-pressure liquid chromatography. the territrem b obtained was treated with alkaline hydrogen peroxide, and 3, 4, 5-trimethoxy benzoic acid was isolated from an ethyl acetate extract of the reaction mixture and purified by thin-layer chromatography and high-pre ... | 1988 | 16347569 |
| susceptibility of environmental versus clinical strains of pathogenic aspergillus. | the objective of this study was to compare the antifungal susceptibility profiles of 307 environmental strains and 139 clinical isolates of aspergillus belonging to six different species. clinical and environmental strains with minimal inhibitory concentrations (mics) or minimal effective concentrations >or=4microg/ml to amphotericin b (amb), itraconazole (itc), voriconazole and caspofungin were seldom detected. however, the susceptibility profile of environmental aspergillus non-fumigatus strai ... | 2007 | 17189101 |
| bioremediation of endosulfan using aspergillus terreus and cladosporium oxysporum. | | 2005 | 16400595 |
| risk factors for pulmonary aspergillus terreus infection in patients with positive culture for filamentous fungi. | invasive aspergillosis (ia) is a common fungal infection in immunocompromised patients and has a high mortality rate. among patients with ia, aspergillus terreus infections have become a growing concern in the past few years. | 2007 | 17218581 |
| modification of the x-ray and ultraviolet induced mutation rate in aspergillus terreus by pretreatment with near infrared radiation. | | 1948 | 17247289 |
| broad substrate cytochrome p450 monooxygenase activity in the cells of aspergillus terreus mtcc 6324. | cytochrome p450 (cyp) monooxygenase activities with different category of substrates namely, alkanes, alkane derivatives, alcohols, aromatic compounds, organic solvents, and steroids were detected in the cells of aspergillus terreus. high cyp specific activity was observed when methanol (5.6+/-0.017 u mg(-1)), acetone (7.76+/-0.02 u mg(-1)), dimethylsulphoxide (dmso) (9.70+/-0.005 u mg(-1)), n-hexadecane (4.39+/-0.02 u mg(-1)), or n-octadecane (4.23+/-0.01 u mg(-1)) were used as substrates. sign ... | 2008 | 17251009 |
| development of novel real-time pcr assays for detection and differentiation of eleven medically important aspergillus and candida species in clinical specimens. | in the present study, novel real-time pcr assays targeting the fungal its2 region were developed for the detection and differentiation of medically important aspergillus species (aspergillus fumigatus, aspergillus flavus, aspergillus nidulans, aspergillus niger, and aspergillus terreus) and candida species (candida albicans, candida dubliniensis, candida glabrata, candida krusei, candida parapsilosis, and candida tropicalis) using a lightcycler instrument. the combination of a group-specific and ... | 2007 | 17251398 |
| hexadecylphosphocholine (miltefosine) has broad-spectrum fungicidal activity and is efficacious in a mouse model of cryptococcosis. | the alkyl phosphocholine drug miltefosine is structurally similar to natural substrates of the fungal virulence determinant phospholipase b1 (plb1), which is a potential drug target. we determined the mics of miltefosine against key fungal pathogens, correlated antifungal activity with inhibition of the plb1 activities (plb, lysophospholipase [lpl], and lysophospholipase-transacylase [lpta]), and investigated its efficacy in a mouse model of disseminated cryptococcosis. miltefosine inhibited sec ... | 2006 | 16436691 |
| effects of the sporulation conditions on the lovastatin production by aspergillus terreus. | the production of biomass and lovastatin by spore-initiated submerged fermentations of aspergillus terreus atcc 20542 was shown to depend on the age of the spores used for inoculation. cultures started from older spores produced significantly higher titers of lovastatin. for example, the lovastatin titer increased by 52% when the spore age at inoculation rose from 9 to 16 days. the lovastatin titer for a spore age of 16 days was 186.5+/-20.1 mg l(-1). the time to sporulation on surface cultures ... | 2006 | 16491374 |
| posttraumatic aspergillus terreus endophthalmitis masquerading as dispersed lens fragments. | a 20-year-old man developed increasing inflammation with a hypopyon 3 weeks after primary repair of a corneal laceration. an occult anterior capsule puncture was suspected as the stimulus for the inflammation. when aspiration of the suspected hydrated lens material was not curative, a vitrectomy with injection of intravitreal antibiotic agents including amphotericin (0.0125 mg) was done the following day; the culture failed to grow fungal organisms. a repeat vitrectomy was performed 1 week later ... | 2007 | 17397754 |
| functional characterization of alcohol oxidases from aspergillus terreus mtcc 6324. | short chain alcohol oxidase (scao), long chain alcohol oxidase (lcao), secondary alcohol oxidase (sao), and aryl alcohol oxidase (aao) activities were localized in the microsome of aspergillus terreus during growth of the fungi on n-hexadecane. zymogram analysis of the microsomes of n-hexadecane-grown cells in polyacrylamide gel electrophoresis showed distinct bands, h4, h3, h2, and h1, in a sequence of their molecular weight (mr) from high to low. the mr of the isozymes corresponding to the ban ... | 2006 | 16547701 |
| studies in the biochemistry of micro-organisms: the metabolic products of aspergillus terreus thom. part ii. two new chlorine-containing mould metabolic products, geodin and erdin. | | 1936 | 16746160 |
| parallel capillary electrophoresis for the quantitative screening of fermentation broths containing natural products. | directed molecular evolution is a recursive process of controlled genetic diversification and functional screening. the success of this approach is dependent on both the quality of the genetic diversity and the ability to accurately screen a large population of individual genetic variants for those having improved function. in this paper, the application of parallel capillary electrophoresis to rapidly quantitate lovastatin production levels by aspergillus terreus mutants is described. a paralle ... | 2005 | 15721810 |
| cardiac aspergillosis with pedunculated mass in the left ventricle. | immunosuppression can allow organisms that are not usually pathogenetic to cause disease; under such circumstances, aspergillus species frequently form large masses of fungal elements. we describe the case of a 12-year-old girl with hematologic remission of leukemia. she had a left ventricular pedunculated mass that was detected by echocardiographic study; at surgery, the presence of aspergillus terreus was confirmed. | 2004 | 15745301 |
| biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase lovb. | the biosynthesis of lovastatin in aspergillus terreus requires two megasynthases. the lovastatin nonaketide synthase, lovb, synthesizes the intermediate dihydromonacolin l using nine malonyl-coenzyme a molecules, and is a reducing, iterative type i polyketide synthase. the iterative type i polyketide synthase is mechanistically different from bacterial type i polyketide synthases and animal fatty acid synthases. we have cloned the minimal polyketide synthase domains of lovb as standalone protein ... | 2007 | 17466016 |
| analysis of the influence of tween concentration, inoculum size, assay medium, and reading time on susceptibility testing of aspergillus spp. | the influence of several test variables on susceptibility testing of aspergillus spp. was assessed. a collection of 28 clinical isolates was tested against amphotericin b, itraconazole, voriconazole, and terbinafine. inoculum size (10(4) cfu/ml versus 10(5) cfu/ml) and glucose supplementation (0.2% versus 2%) did not have significant effects on antifungal susceptibility testing results and higher inoculum size and glucose concentration did not falsely elevate mics. in addition, antifungal suscep ... | 2005 | 15750092 |
| a colorimetric and spectrophotometric method for in vitro susceptibility testing of aspergillus species against caspofungin. | the in vitro susceptibility of 45 aspergillus fumigatus, aspergillus flavus and aspergillus terreus isolates against caspofungin (cas) was assessed by the clsi reference method with spectrophotometric reading and by a colorimetric method that employed the dye mtt. perfect agreement was found between the minimal effective concentrations (mecs) and the mic-2 values (50% growth reduction) obtained by the mtt method. the agreement found between the mics obtained by the clsi and the mtt method was de ... | 2007 | 17510858 |
| amphotericin b lipid complex in the management of invasive aspergillosis in immunocompromised patients. | the efficacy and renal safety of amphotericin b lipid complex (ablc) were assessed in 398 patients with invasive aspergillosis. the most common underlying conditions were hematopoietic stem-cell transplantation (101/398 [25%]), hematologic malignancy (101/398 [25%]), and solid-organ transplantation (109/398 [27%]). the most common reason for administration of ablc was lack of response to prior antifungal therapy. overall, 65% of patients had a favorable clinical response: 44% were cured or impro ... | 2005 | 15809925 |
| extracellular abts-oxidizing activity of autochthonous fungal strains from argentina in solid medium. | the screening for extracellular oxidases and peroxidases from autochthonous filamentous fungi isolated from different substrates is an important step towards the detection of extracellular fungal oxidative systems. thirty-one autochthonous fungal strains from argentina, belonging to different ecophysiological and taxonomic groups, were plate-screened for their ability to produce extracellular oxidoreductases. modified kirk solid medium containing the chromogen 2,2-azino-bis(3-ethylbenzothiazolin ... | 2000 | 15813698 |
| diagnostic value of dna, (1-3)-beta-d-glucan, and galactomannan detection in serum and bronchoalveolar lavage of mice experimentally infected with aspergillus terreus. | the aim of this study was to evaluate the diagnostic value of aspergillus terreus-specific dna, (1-3)-beta-d-glucan (bdg), and galactomannan (gm) in immunosuppressed mice infected intravenously with a. terreus conidia and sacrificed in groups of 12 each on days 1, 3, 5, 7, and 9. a. terreus-specific dna, bdg, and gm in serum and bronchoalveolar lavage (bal) were detected by nested polymerase chain reaction (npcr), fungitell kit (associates of cape cod, e. falmouth, ma), and aspergillus platelia ... | 2007 | 17574786 |
| use of fluorescent probes to determine mics of amphotericin b and caspofungin against candida spp. and aspergillus spp. | we investigated the utility of mechanism-based fluorescent probes for determination of mics (fmics) of amphotericin b and caspofungin against candida spp. and aspergillus spp. amphotericin b was selected as a membrane-active antifungal agent, and caspofungin was selected as a cell wall-active agent. fmics were also compared to the mic determined by clsi (formerly nccls) methods. five isolates per species of candida albicans, candida glabrata, candida parapsilosis, aspergillus fumigatus, and aspe ... | 2005 | 16081911 |
| [aspergillus terreus infection in copd patients receiving corticoids. a report of three cases]. | | 2007 | 17583657 |
| studies on the application of immobilization technique for the production of cyclosporin a by a local strain of aspergillus terreus. | the formation of cyclosporin a (cy a) by immobilized spores and mycelia of aspergillus terreus was investigated. different carriers were tested as immobilizing carriers, whereby ca-alginate was selected for further experimentation. the role of alginate concentration, biomass weight, ph value of the cultivation medium, repeated utilization of the immobilized fungus as well as the supplementation of different amino acid precursors were studied. best cy a outputs were attained with ca-alginate 3% ( ... | 2005 | 16107751 |
| sesterterpenoids, terretonins a-d, and an alkaloid, asterrelenin, from aspergillus terreus. | four new sesterterpenoids, terretonins a-d (1-4), and a new alkaloid, asterrelenin (5), together with five known compounds, were isolated from the ethyl acetate extract of a solid-state fermented culture of aspergillus terreus. their structures were elucidated on the basis of spectroscopic analysis. the structures of 1, 2, and 5 were confirmed by x-ray crystallographic analysis. | 2005 | 16124769 |
| simultaneous biosynthesis of (+)-geodin by a lovastatin-producing fungus aspergillus terreus. | the simultaneous biosynthesis of lovastatin (mevinolinic acid) and (+)-geodin by aspergillus terreus atcc 20542 with regard to the medium composition, i.e. the concentrations of carbon and nitrogen source, was described in this paper. a. terreus is a lovastatin producer but the formation of lovastatin was accompanied by the significant amounts of (+)-geodin, when the elevated concentration of carbon source (lactose) was still present in the medium in the idiophase and nitrogen source (yeast extr ... | 2007 | 17689800 |
| micafungin in combination with voriconazole in aspergillus species: a pharmacodynamic approach for detection of combined antifungal activity in vitro. | preclinical and clinical evidence indicate that echinocandin-triazole combinations provide enhanced killing versus triazoles alone against some aspergillus isolates, however, in vitro test results designed to detect this combined effect are difficult to interpret. | 2005 | 16188916 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| epidemiology and outcome of infections due to aspergillus terreus: 10-year single centre experience. | aspergillus terreus, a less common pathogen, appears to be an emerging cause of infection at our institution, the medical university hospital of innsbruck. thus the epidemiology and outcome of a. terreus infections over the past 10 years was assessed. we analysed 67 cases of proven invasive aspergillosis (ia) according to the european organisation for research and treatment of cancer/mycoses study group criteria, investigated antifungal susceptibility of amphotericin b (amb), voriconazole and ca ... | 2005 | 16197450 |
| the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species. | in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ... | 2007 | 17761478 |
| effect of caspofungin on metabolite profiles of aspergillus species determined by nuclear magnetic resonance spectroscopy. | invasive aspergillosis remains a potentially life-threatening infection, the incidence of which is increasing. current methods used to determine the susceptibilities of aspergillus strains to antifungal drugs are often unreliable. nuclear magnetic resonance (nmr) spectroscopy can identify the metabolic complement of microorganisms while monitoring nutrient utilization from the incubation medium. we used 600-mhz (1)h nmr spectroscopy to monitor the metabolic responses of five aspergillus species ... | 2007 | 17785514 |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| supplementation of the cultivation media with b-group vitamins enhances lovastatin biosynthesis by aspergillus terreus. | the impact of the supplementation of cultivation media with b-group vitamins on the biosynthesis of lovastatin (mevinolinic acid) by aspergillus terreus atcc20542 was investigated. a hypothesis was formulated that as the biosynthesis of lovastatin requires a high throughput of coenzymes in the cells, the application of its precursors in the form of b-group vitamins might positively influence the process. in a nitrogen-deficient medium the b-group vitamins, both single, especially nicotinamide, p ... | 2007 | 16887228 |
| susceptibility of fungi isolated from the respiratory tract of falcons to amphotericin b, itraconazole and voriconazole. | the minimum inhibitory concentrations (mics) of fungi isolated from the air sacs of falcons before (group 1), and during antifungal treatment with amphotericin b nebulisation and oral itraconazole or voriconazole (group 2), or with itraconazole alone (group 3) or voriconazole alone (group 4) were determined. before treatment, 95 per cent of the isolates, including aspergillus fumigatus, aspergillus flavus, aspergillus niger and aspergillus terreus, were susceptible to voriconazole at mics up to ... | 2006 | 16946311 |
| enzymatic characteristics of ligninperoxidases from penicillium citrinum, fusarium oxysporum and aspergillus terreus using n-propanol as substrate. | the activities of ligninperoxidases from penicillium citrinum mtcc 3565, fusarium oxysporum mtcc 3379 and aspergillus terreus mtcc 3374 have been assayed and the enzymatic characteristics like km, ph and temperature optima using n-propanol as the substrate have been reported. the results suggest that n-propanol can substitute veratryl alcohol as substrate for assaying ligninperoxidase activities from different fungal strains, without affecting the enzymatic characteristics. the above strains wer ... | 2006 | 16955752 |
| early detection of aspergillus terreus in a tenckhoff catheter. | | 2006 | 17047247 |
| secondary sternal aspergillus osteomyelitis in a diabetic hemodialysis patient with previous allograft rejection. | a 29-year-old diabetic woman who had a previously failed renal allograft on maintenance hemodialysis developed sternal aspergillosis with aspergillus terreus following a pericardiectomy. she was successfully treated with surgical debridement and a combination of antifungal agents including amphotericin b, caspofungin, and voriconizole. the diagnostic difficulties and management are discussed. | 2007 | 17922735 |
| antifungal interactions within the triple combination of amphotericin b, caspofungin and voriconazole against aspergillus species. | the in vitro effects of caspofungin combined with voriconazole and amphotericin b were tested in triplicate experiments against nine clinical isolates of aspergillus fumigatus, aspergillus flavus and aspergillus terreus. | 2006 | 17071635 |
| in vitro susceptibilities of aspergillus spp. causing otomycosis to amphotericin b, voriconazole and itraconazole. | otomycosis is worldwide in distribution and most commonly caused by aspergillus species. amphotericin b, itraconazole and voriconazole are used for the treatment of aspergillosis, but recently an increase in resistance to these agents has been reported. we aimed at investigating the in vitro activities of amphotericin b, voriconazole and itraconazole against aspergillus isolates causing otomycosis. mycological analysis of samples from the ear canals of patients was performed by culturing onto sa ... | 2007 | 17944704 |
| antimicrobial activity of some important adiantum species used traditionally in indigenous systems of medicine. | adiantum linn. of adiantaceae family is one of the most common and widely distributed species. ethnomedicinally, the genus is important and popularly known as "hansraj" in ayurvedic system of medicine. it has been used in cold, tumors of spleen, liver and other viscera, skin diseases, bronchitis and inflammatory diseases. it is also considered as tonic and diuretic. in the present study its four important species, i.e. adiantum capillus-veneris, adiantum peruvianum, adiantum venustum and adiantu ... | 2008 | 17997240 |
| processing of soybean products by semipurified plant and microbial alpha-galactosidases. | galactooligosaccharides (go) are responsible for intestinal disturbances following ingestion of legume-derived products. enzymatic reduction of go level in these products is highly desirable to improve their acceptance. for this purpose, plant and microbial semipurified alpha-galactosidases were used for go hydrolysis in soybean flour and soy molasses. alpha-galactosidases from soybean germinating seeds, aspergillus terreus, and penicillium griseoroseum presented maximal activities at ph 4.0-5.0 ... | 2006 | 17177558 |
| in-vitro susceptibility testing by agar dilution method to determine the minimum inhibitory concentrations of amphotericin b, fluconazole and ketoconazole against ocular fungal isolates. | to standardize in-vitro antifungal susceptibility testing by agar dilution method to find out the minimum inhibitory concentration (mic) of amphotericin b, fluconazole and ketoconazole on ocular fungal isolates. | 2006 | 17185846 |
| caspofungin as primary antifungal prophylaxis in stem cell transplant recipients. | to assess the effectiveness and tolerability of caspofungin as primary prophylaxis against invasive fungal infections in stem cell transplant recipients who are poor candidates for triazole or lipid amphotericin b prophylaxis due to renal or hepatic dysfunction, and to determine whether any patient characteristics are independently associated with an increased risk of breakthrough invasive fungal infection during caspofungin prophylaxis. | 2007 | 18041885 |
| syntheses of conjugated pyrones for the enzymatic assay of lovastatin nonaketide synthase, an iterative polyketide synthase. | [reaction: see text] lovastatin nonaketide synthase (lovb, lnks) is an iterative type i polyketide synthase (pks) from aspergillus terreus that produces dihydromonacolin l (2), the biosynthetic precursor of lovastatin (1), from acetyl coa, malonyl coa, and s-adenosylmethionine (sam) if the accessory protein lovc is present. in the absence of lovc, lnks forms the conjugated pyrones 3 and 4 as truncated pks products. short syntheses of these pyrones provide material for the assay of lnks activity ... | 2007 | 17192110 |
| involvement of tetrahymena pyriformis and selected fungi in the elimination of anthracene, and toxicity assessment of the biotransformation products. | anthracene (ac) is a non-mutagenic and non-carcinogenic, low-molecular-weight polycyclic aromatic hydrocarbon present in the environment. its toxicity can be dramatically increased after solar-light exposure. biotransformation capacities of ac by tetrahymena pyriformis and a selection of eight micromycetes were studied, and the ability of these microorganisms to detoxify the polluted ecosystems was assessed. we showed that t. pyriformis was able to accumulate high amounts of ac without any trans ... | 2008 | 17257678 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| x-ray crystal structure of aristolochene synthase from aspergillus terreus and evolution of templates for the cyclization of farnesyl diphosphate. | aristolochene synthase from aspergillus terreus catalyzes the cyclization of the universal sesquiterpene precursor, farnesyl diphosphate, to form the bicyclic hydrocarbon aristolochene. the 2.2 a resolution x-ray crystal structure of aristolochene synthase reveals a tetrameric quaternary structure in which each subunit adopts the alpha-helical class i terpene synthase fold with the active site in the "open", solvent-exposed conformation. intriguingly, the 2.15 a resolution crystal structure of t ... | 2007 | 17261032 |
| activities of voriconazole, itraconazole and amphotericin b in vitro against 590 moulds from 323 patients in the voriconazole phase iii clinical studies. | fungal pathogens from the voriconazole trials were identified and tested for susceptibility at two reference laboratories. | 2008 | 18222957 |
| peritonitis due to aspergillus terreus in a patient undergoing continuous ambulatory peritoneal dialysis. | aspergillus peritonitis is a rare, potentially fatal complication of continuous ambulatory peritoneal dialysis (capd). we report a case of capd peritonitis caused by aspergillus terreus in a 55-year-old female patient. | 2008 | 18254757 |
| a comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. | the antioxidant, antifungal and antibacterial potentials of volatile oils and oleoresin of cinnamomum zeylanicum blume (leaf and bark) were investigated in the present study. the oleoresins have shown excellent activity for the inhibition of primary and secondary oxidation products in mustard oil added at the concentration of 0.02% which were evaluated using peroxide, thiobarbituric acid, p-anisidine and carbonyl values. moreover, it was further supported by other complementary antioxidant assay ... | 2007 | 17408833 |
| potential basis for amphotericin b resistance in aspergillus terreus. | this study investigated the basis for intrinsic amphotericin b (amb) resistance in aspergillus terreus. the ergosterol content, cell wall composition, and lipid peroxidation level had no influence on amb resistance. the level of catalase production in a. terreus was significantly higher than that in a. fumigatus (p < 0.05). this higher-level production may contribute to amb resistance in a. terreus since oxidative damage has been implicated in amb action. | 2008 | 18268082 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| itaconic acid production using sago starch hydrolysate by aspergillus terreus tn484-m1. | sago starch was hydrolyzed using either chemical agents, or enzymes at various ph and concentrations. hydrolysis using 5000 aun/ml (0.5%, w/v) glucoamylase exhibited the highest itaconic acid yield up to 0.36 g/g sago starch, whereas hydrolysis using nitric acid at ph 2.0 yielded 0.35 g/g sago starch. the medium was optimized and the composition was (g/l) 140 sago starch, 1.8 corn steep liquor, 1.2 mgso(4).7h(2)o and 2.9 nh(4)no(3). when the optimal conditions of hydrolysis and medium compositio ... | 2007 | 17451943 |
| exposure to airborne fungi during conservative dental treatment. | the aim of the study was a mycological assessment of bioaerosol forming during conservative dental treatment, taking into account concentration and type of fungal microflora, and evaluation of the influence of duwl disinfecting protocol on the fungal contamination of the bioaerosol. the research was conducted on 25 operative sites located in public dental clinics. the air contained in the space between a patient and a dentist during conservative dental treatment with the use of a high-speed hand ... | 2006 | 16841889 |
| voriconazole in the management of nosocomial invasive fungal infections. | voriconazole is a new triazole developed for the treatment of life-threatening fungal infections. the drug is available for both oral and intravenous administration; the oral formulation has excellent bioavailability. the side-effect profile of voriconazole presents an acceptable safety and tolerability spectrum: transient visual disturbances, liver enzyme abnormalities, and skin rashes are the most frequently reported side effects but rarely lead to discontinuation. the potential for drug-drug ... | 2006 | 18360588 |
| in vitro determination of phagocytosis and intracellular killing of aspergillus species by mononuclear phagocytes. | we investigated phagocytosis and intracellular killing of clinical and environmental isolates of aspergillus spp. by human monocyte-derived macrophages (mdms). serial pathogens such as aspergillus fumigatus, aspergillus flavus and aspergillus terreus were examined with a microbiological assay. phagocytosis for resting conidia of aspergillus spp. was similar for all isolates tested. during 30 min of incubation phagocytosis ranged from 49.9% to 85.5% for clinical isolates and from 40.3% to 87.1% f ... | 2007 | 17458710 |
| influence of amphotericin b and amphotericin b colloidal dispersion on the functions of human phagocytes in defence against aspergillus species. | this study investigated the in vitro effects of amphotericin b and amphotericin b colloidal dispersion (abcd) on phagocytosis and inhibition of germination of clinical isolates of aspergillus spp. by monocyte-derived macrophages (mdms). both amphotericin b and abcd caused significant reductions in uptake of conidia of aspergillus spp. by mdms (p < 0.01). the inhibition of germination was superior with conidia of aspergillus fumigatus, aspergillus niger and aspergillus terreus isolates. aspergill ... | 2007 | 17486378 |
| invasive pulmonary aspergillosis with hematological malignancy caused by aspergillus terreus and in vitro susceptibility of a. terreus isolate to micafungin. | a 35-year-old man developed invasive pulmonary aspergillosis (ipa) with severe neutropenia after umbilical cord stem cell transplantation for chronic myelogenous leukemia. filamentous fungus isolated from his sputum was identified as aspergillus terreus. despite systemic amphotericin b (amph) administration, ipa progressed. however, intravenous administration of micafungin (mcfg) and oral itraconazole improved clinical data and symptoms, although he later died of massive hemoptysis. examination ... | 2007 | 17541234 |
| investigation into the production of 2-pentylfuran by aspergillus fumigatus and other respiratory pathogens in vitro and human breath samples. | our objective was to identify, for diagnostic purposes, potential volatile biomarkers of human microbial pathogens. we analysed the head space of cultures of medically important bacterial and fungal respiratory pathogens for 2-pentylfuran (2pf) production through the use of solid phase micro extraction (spme) and gas chromatography/mass spectroscopy (gc/ms). our results confirm that 2pf is consistently produced by aspergillus fumigatus, fusarium spp., aspergillus terreus, aspergillus flavus and ... | 2008 | 18404548 |
| [construction of a double functional recombinant strain of pichia pastoris co-expressing phytase and mannanase and the enzymatic analyses]. | co-expression of phytase and mannanase in pichia pastoris is a useful way to reduce the production cost in feedstuff industry. based on the published dna sequences of phytase gene and mannanase gene, primers were designed and genes phya and man were cloned by pcr from aspergillus terreus and the plasmid phbm1201, respectively. then the two fragments were treated and inserted into the same expression vector phbm907c, which contains both the methanol-inducible promoter and the transcription termin ... | 2007 | 17552235 |
| molecular typing of aspergillus terreus isolates collected in houston, texas, and innsbruck, austria: evidence of great genetic diversity. | aspergillus terreus isolates collected from patients at the m. d. anderson cancer center in houston, tx, and at the university hospital of innsbruck, austria, were analyzed using random amplification of polymorphic dna-pcr with three different primers. no strain similarity in either institution was detected, indicating great genetic diversity of a. terreus. | 2007 | 17581930 |
| apparent mrna instability in aspergillus nidulans and aspergillus terreus of a heterologous cdna encoding the major capsid antigen of rotavirus. | two expression plasmids designed to produce the rotaviral vp6 protein in aspergillus nidulans and aspergillus terreus have been constructed. in one of these plasmids the inducible a. terreus gla1 glucoamylase gene promoter and gla1 signal sequence are fused to the vp6 cdna to enable induction and extracellular secretion of the final protein product; in the other, the strong, constitutive a. nidulans gpda gene promoter has been employed. a. nidulans and a. terreus transformants containing intact ... | 1999 | 18473559 |
| a macrokinetic modelling of the biosynthesis of lovastatin by aspergillus terreus. | in this work a simple kinetic model to describe the biosynthesis of lovastatin by aspergillus terreus atcc 20542 was proposed. several series of experiments were conducted at different media compositions. the concentrations of c- and n-sources were changed over a wide range and so were the initial biomass concentrations. from these runs the relationships ruling the substrates uptake, biomass and product formation were learnt. lovastatin biosynthesis appeared to be partly growth associated. the i ... | 2007 | 17602773 |