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sclerosing mediastinitis caused by aspergillus terreus.a case of aspergillus terreus causing sclerosing mediastinitis which presented with symptoms of cardiorespiratory compromise and compressive myelopathy is described. the diagnosis was established by culturing and isolating the fungus in pure culture from the tissue and was also confirmed by demonstration of sepcific precipitating antibodies against aspergillus terreus in patient's serum.200111883130
removal of heavy metals by an aspergillus terreus strain immobilized in a polyurethane matrix.the aim was to investigate the biosorption of chromium, nickel and iron from metallurgical effluents, produced by a steel foundry, using a strain of aspergillus terreus immobilized in polyurethane foam.200211849492
biosynthesis and biotechnological production of statins by filamentous fungi and application of these cholesterol-lowering drugs.hypercholesterolemia is considered an important risk factor in coronary artery disease. thus the possibility of controlling de novo synthesis of endogenous cholesterol, which is nearly two-thirds of total body cholesterol, represents an effective way of lowering plasma cholesterol levels. statins, fungal secondary metabolites, selectively inhibit hydroxymethyl glutaryl-coenzyme a (hmg-coa) reductase, the first enzyme in cholesterol biosynthesis. the mechanism involved in controlling plasma chole ...200211956737
common environmental allergens causing respiratory allergy in india.respiratory allergy affects all age groups but the children are the worst affected by the respiratory allergy. bioparticles from different biological sources are the main cause of allergy. pollen grains, fungal spores, insect and other materials of biological origin form the most important allergen load in the air. for the efficient diagnosis of the allergy and its effective treatment it is very important to know about the prevalence, seasonal and annual variations of aeroallergens of the area. ...200212003301
mixed submerged fermentation with two filamentous fungi for cellulolytic and xylanolytic enzyme production.the efficient saccharification of lignocellulosic materials requires the cooperative actions of different cellulase enzyme activities: exoglucanase, endoglucanase, beta-glucosidase, and xylanase. previous studies with the fungi strains aureobasidium sp. chte-18, penicillium sp. ch-te-001, and aspergillus terreus ch-te-013, selected mainly because of their different cellulolytic and xylanolytic activities, have demonstrated the capacity of culture filtrates of cross-synergistic action in the sacc ...200212018234
aspergillus cholangitis: a late complication after kasai portoenterostomy.ascending bacterial cholangitis can be a recurring complication in older children and adolescents who have undergone successful surgery for biliary atresia. the authors describe a 19-year-old girl with a history of recurrent late-onset, febrile cholangitis in whom persistent jaundice developed. aspergillus terreus was isolated from her biliary fluid obtained at percutaneous transhepatic cholangiography. despite prolonged external biliary drainage and systemic amphotericin b therapy, the fungus w ...200212037766
in vitro studies on the activity of amphotericin b and lipid-based amphotericin b formulations against aspergillus conidia and hyphae.the minimum inhibitory concentrations (mics) of amphotericin b and lipid-based amphotericin b formulations against isolates of aspergillus spp. were tested using a broth microdilution method. twelve isolates of aspergillus fumigatus, eight of aspergillus flavus, six of aspergillus niger and seven of aspergillus terreus were examined. in addition, an assay for hyphae of aspergillus spp. was performed since the invasive form is manifested by the appearance of hyphal structures. mics of hyphae agai ...200212100533
a novel streptomycete lipase: cloning, sequencing and high-level expression of the streptomyces rimosus gds(l)-lipase gene.an extracellular lipase from streptomyces rimosus r6-554w has been recently purified and biochemically characterized. in this report the cloning, sequencing, and high-level expression of its gene is described. the cloned dna contained an orf of 804 bp encoding a 268-amino-acid polypeptide with 34 amino acid residues at the amino terminus of the sequence that were not found in the mature protein. the theoretical molecular mass (24.172 kda) deduced from the amino acid sequence of the mature enzyme ...200212115057
enhanced production of itaconic acid from corn starch and market refuse fruits by genetically manipulated aspergillus terreus skr10.a potent itaconic acid producing strain, aspergillus terreus skr10, was isolated from horticulture waste. market refuse, apple and banana, were explored as novel substrates for itaconic acid production with yields of 20+/-2.0 and 20.0+/-1.0 g l(-1), respectively. itaconic acid yields of 28.5+/-2.2 and 31.0+/-1.7 g l(-1) were obtained with acid and alpha-amylase hydrolyzed corn starch. the efficiency of itaconic acid production by this wild type strain was improved by ultraviolet, chemical and mi ...200212146646
antifungal drug resistance to azoles and polyenes.there is an increased awareness of the morbidity and mortality associated with fungal infections caused by resistant fungi in various groups of patients. epidemiological studies have identified risk factors associated with antifungal drug resistance. selection pressure due to the continuous exposure to azoles seems to have an essential role in developing resistance to fluconazole in candida species. haematological malignancies, especially acute leukaemia with severe and prolonged neutropenia, se ...200212206971
thermal inactivation and product inhibition of aspergillus terreus cect 2663 alpha-l-rhamnosidase and their role on hydrolysis of naringin solutions.the kinetics of thermal inactivation of a. terreus alpha-rhamnosidase was studied using the substrate p-nitrophenyl alpha-l-rhamnoside between 50 degrees c and 70 degrees c. up to 60 degrees c the inactivation of the purified enzyme was completely reversible, but samples of crude or partially purified enzyme showed partial reversibility. the presence of the product rhamnose, the substrate naringin, and other additives reduced the reversible inactivation, maintaining in some cases full enzyme act ...200212224626
aspterric acid and 6-hydroxymellein, inhibitors of pollen development in arabidopsis thaliana, produced by aspergillus terreus.aspterric acid, 6-hydroxymellein, arabidopsis thaliana, aspergillus terreus aspterric acid (1) and 6-hydroxymellein (2), inhibitors of pollen development in arabidopsis thaliana, have been isolated from the fungus aspergillus terreus. 1 and 2 inhibited the pollen development at concentrations of 38 and 52 microm, respectively. the microscopic examination of pollen development suggested that the inhibition by the treatment with 1 caused at meiosis and the inhibition by the treatment with 2 caused ...200212132685
detection of cell wall galactomannoprotein afmp1p in culture supernatants of aspergillus fumigatus and in sera of aspergillosis patients.mannoproteins are important and abundant structural components of fungal cell walls. the afmp1 gene encodes a cell wall galactomannoprotein of aspergillus fumigatus. in the present study, we show that afmp1p is secreted into the cell culture supernatant at a level that can be detected by western blotting. a sensitive enzyme-linked immunosorbent assay (elisa) developed with antibodies against afmp1p was capable of detecting this protein from the cell culture supernatant of a. fumigatus. the anti- ...200212409437
in vitro interaction of terbinafine with itraconazole, fluconazole, amphotericin b and 5-flucytosine against aspergillus spp.we investigated the in vitro interaction of terbinafine with itraconazole, fluconazole, amphotericin b and 5-flucytosine, against aspergillus spp. we tested three isolates of aspergillus fumigatus (one resistant to itraconazole), and two each of aspergillus flavus, aspergillus niger and aspergillus terreus. we employed a broth microdilution-based method derived from an in vivo validated method capable of detecting itraconazole resistance in a. fumigatus. we studied the effect on the mics by calc ...200212161398
invasive aspergillosis caused by aspergillus terreus in a non-immunosuppressed patient. 200212421042
molecular cloning and characterization of an ml-236b (compactin) biosynthetic gene cluster in penicillium citrinum.cloning of genes encoding polyketide synthases (pkss) has allowed us to identify a gene cluster for ml-236b biosynthesis in penicillium citrinum. like lovastatin, which is produced by aspergillus terreus, ml-236b (compactin) inhibits the enzyme 3-hydroxy-3-methylglutaryl-coenzyme a (hmg-coa) reductase. genomic sequencing and northern analysis showed that nine predicted genes for ml-236b biosynthesis were located within a 38-kb region and were transcribed when ml-236b was produced. the predicted ...200212172803
production of lovastatin examined by an integrated approach based on chemometrics and dosy-nmr.microbial secondary metabolites are one of the sources of therapeutic molecules in the pharmaceutical industry. product quality and high yields of secondary metabolites are the main goals for the commercial success of a fermentation process. our novel approach was based on the decision-tree algorithm to determine the key variables correlated with the process outcome and on dosy-nmr to identify both co-metabolites and impurities, and it improves fermentation systems and speeds up bioprocess devel ...200212355470
aspergillus terreus in a cystic fibrosis clinic: environmental distribution and patient colonization pattern. 200312495692
identification of a single nuclear localization signal in the c-terminal domain of an aspergillus dna topoisomerase ii.dna topoisomerase ii (topo ii) is a major nuclear protein that plays an important role in dna metabolism. we have isolated the gene for topo ii ( top2) from the filamentous fungus aspergillus terreus. the deduced amino acid sequence revealed that topo ii consists of 1,587 amino acids and has a calculated molecular weight of 180 kda; the protein expressed in escherichia coli has an estimated molecular weight of 185 kda. expression of topo ii polypeptides tagged with yellow fluorescent protein (yf ...200212436251
characterisation and application of glycanases secreted by aspergillus terreus ccmi 498 and trichoderma viride ccmi 84 for enzymatic deinking of mixed office wastepaper.two enzymatic extracts obtained from xylan-grown aspergillus terreus ccmi 498 and cellulose-grown trichoderma viride ccmi 84 were characterised for different glycanase activities. both strains produce extracellular endoxylanase and endoglucanase enzymes. the enzymes optimal activity was found in the temperature range of 45-60 degrees c. endoglucanase systems show identical activity profiles towards temperature, regardless of the strain and inducing substrate. conversely, the endoxylanases produc ...200312443852
[mutation site-specific assay (mssa) for the detection of hepatitis b virus precore defective mutant in hepatitis b infection]. 199512442404
detection and identification of fungi from fungus balls of the maxillary sinus by molecular techniques.the aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. one hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-embedded tissue sections of the maxillary sinus. in 31 cases, sinus contents without paraffin embedding were sent for investigation. pcr amplification with universal fungal primers ...200312574250
secretion of ligninperoxidase by penicillium citrinum, fusarium oxysporum and aspergillus terreus.secretion of ligninperoxidase [e.c.1.11.1.7] by penicillium citrinum, fusarium oxysporum and aspergillus terreus in liquid culture growth medium has been demonstrated. enzymatic characteristics like km, ph and temperature optima using veratryl alcohol as the organic substrate of ligninperoxidases from above sources have been determined. km values using veratryl alcohol as substrate for enzymes from p. citrinum, f. oxysporum and a. terreus were 69, 64 and 60 microm respectively. km values using h ...200212597550
bioconversion of poultry wastes. i--factors influencing the assay and productivity of crude uricase by three uricolytic filamentous fungi.the optimum temperature for biomass yield and uricase production by uricolytic fungi, aspergillus terreus, a. flavus and trichoderma sp. was at 30 degrees c. the time required for maximum production of uricase and biomass yield was 4 days for two aspergillus species and 6 days for trichoderma sp. the optimum ph was at 6.4 for a. terreus and ph 6.6 for a. flavus and trichoderma sp. the maximum fungal biomass yield was achieved in medium supplemented with 4% poultry waste. the best carbon sources ...200212512254
integrating transcriptional and metabolite profiles to direct the engineering of lovastatin-producing fungal strains.we describe a method to decipher the complex inter-relationships between metabolite production trends and gene expression events, and show how information gleaned from such studies can be applied to yield improved production strains. genomic fragment microarrays were constructed for the aspergillus terreus genome, and transcriptional profiles were generated from strains engineered to produce varying amounts of the medically significant natural product lovastatin. metabolite detection methods wer ...200312536215
[determination of itaconic acid in culture broth by high performance liquid chromatography].an hplc method for the determination of itaconic acid and other components in the culture broth of aspergillus terreus was developed. the operating conditions were aminex hpx-87h column (300 mm x 7.8 mm) at 30 degrees c, mobile phase of sulfuric acid (4 mmol/l) and refractive index detector at 30 degrees c. the linear relationship of calibration curve was good in the range of 0-50 g/l for itaconic acid (r = 0.9997). the detection limit was 5.7 micrograms, and the relative standard deviation was ...199912552860
invasive synergistic fungal infection after motor vehicle collision.primary invasive fungal infections occur after direct contact or direct inoculation of the skin with fungal spores. rhizopus species and aspergillus terreus are opportunistic fungal species that rarely cause disease in immunocompetent hosts. in susceptible patients, infection may progress rapidly. aggressive surgical debridement and use of systemic antimycotic agents may successfully control disease and prevent systemic dissemination. we describe the case of a patient with a scalp infection, cau ...200312661747
activity of micafungin (fk463) against an itraconazole-resistant strain of aspergillus fumigatus and a strain of aspergillus terreus demonstrating in vivo resistance to amphotericin b.we compared the activity of four doses of micafungin (fk463) with that of amphotericin b, liposomal amphotericin b and itraconazole in a murine model of disseminated aspergillosis. temporarily neutropenic mice were infected with a lethal dose of either an itraconazole-resistant aspergillus fumigatus isolate or aspergillus terreus, a species that is less susceptible to amphotericin b. treatment was started 24 h after infection and lasted for 7 days. mice were treated with either amphotericin b (0 ...200312654746
peritonitis due to aspergillus and zygomycetes in patients undergoing peritoneal dialysis: report of 2 cases and review of the literature.fungal peritonitis in patients on continuous ambulatory peritoneal dialysis (capd) has been associated with high mortality and high capd-discontinuation rates. most cases are due to candida spp. while aspergillus spp. and zygomycetes have only rarely been implicated. we report one case each of capd-related peritonitis caused by aspergillus terreus and mucor sp., which have previously been described in the literature once and twice, respectively. the former had a slowly progressive course, did no ...200312742319
in vitro susceptibility of aspergillus spp. clinical isolates to albendazole.the in vitro antifungal activity of albendazole, a benzimidazole widely used as an antihelmintic drug in humans, was investigated and assessed for its activity against aspergillus spp. forty-eight isolates, representing the most frequent species found in human pathology [aspergillus fumigatus (n = 27), aspergillus flavus (n = 10), aspergillus terreus (n = 7), aspergillus nidulans (n = 3) and aspergillus niger (n = 1)], and one quality control strain (a. niger atcc 9804 83435) were tested accordi ...200312716778
effect of increasing inoculum sizes of aspergillus hyphae on mics and mfcs of antifungal agents by broth microdilution method.in order to investigate the influence of different hyphal inoculum sizes on minimal inhibition concentrations (mics) and minimum fungicidal concentrations (mfcs) of amphotericin b (amb), voriconazole and itraconazole, five isolates each of aspergillus fumigatus, aspergillus flavus, aspergillus niger and aspergillus terreus were studied using a broth microdilution method. three inoculum sizes were used: 1 x 10(3)-5 x 10(3), 1 x 10(4)-5 x 10(4) and 1 x 10(5)-5 x 10(5) cfu/ml. mics and mfcs were re ...200312636983
terreulactones a, b, c, and d: novel acetylcholinesterase inhibitors produced by aspergillus terreus. i. taxonomy, fermentation, isolation and biological activities.in the course of screening for selective inhibitors of acetylcholinesterase from the microbial metabolites, four new meroterpenoid compounds, terreulactones a, b, c and d were isolated from solid state fermentation of aspergillus terreus fb000501. they showed potent inhibitory activities against acetylcholinesterase with ic50 values in range of 0.06 to approximately 0.42 microm. in addition, they exhibited more than 500 to approximately 3000 times selectivity for acetylcholinesterase compared wi ...200312817807
terreulactones a, b, c, and d: novel acetylcholinesterase inhibitors produced by aspergillus terreus. ii. physico-chemical properties and structure determination.terreulactones a, b, c, and d, new meroterpenoid compounds with potent anti-acetylcholinesterase activity, were determined to be mixed polyketides-terpenoid structures by spectroscopic studies.200312817808
acute myelogenous leukemia complicated by acute necrotizing ulcerative gingivitis due to aspergillus terreus.infections caused by aspergillus terreus are rare but have been associated with a poor outcome in immunocompromised patients due to frequent resistance to conventional antifungal therapy. this report describes a case of a woman who developed acute necrotizing ulcerative gingivitis (anug) due to a. terreus during induction chemotherapy for acute myelogenous leukemia. she initially failed to respond to treatment with amphotericin b but the infection resolved following the introduction of oral itra ...200312769350
faster genetic identification of medically important aspergilli by using gellan gum as gelling agent in mycological media.using gellan gum as a substitute for agar-agar in a mycological medium and sequencing of the its 1 and 2 regions resulted in an accurate identification of aspergillus fumigatus, aspergillus nidulans, aspergillus terreus and aspergillus ustus within 24 h of subculture.200312867558
activities of caspofungin, itraconazole, posaconazole, ravuconazole, voriconazole, and amphotericin b against 448 recent clinical isolates of filamentous fungi.we examined the in vitro activity of caspofungin, posaconazole, voriconazole, ravuconazole, itraconazole, and amphotericin b against 448 recent clinical mold isolates. the endpoint for reading caspofungin was the minimum effective concentration (mec). among the triazoles, posaconazole was most active, inhibiting 95% of isolates at <or=1 microg/ml, followed by ravuconazole (91%), voriconazole (90%), and itraconazole (79%). caspofungin and amphotericin b inhibited 93% and 89% of isolates at <or=1 ...200312904365
diagnosis of invasive septate mold infections. a correlation of microbiological culture and histologic or cytologic examination.we correlated results of microbiologic culture and histopathologic examination for 2,891 consecutive samples from autopsy tissue, surgical or biopsy tissue, and bronchoalveolar lavage (bal) or bronchial washing (bw) specimens. for 23 autopsy cases with suspected invasive septate mold infections by histopathologic examination, culture yielded a mold in 12 cases (52%). for 1,683 surgical or biopsy samples, histopathologic evidence of invasive septate mold infection was present in 30 samples, 9 of ...200312817433
prominent induction of cyclin b1 in g2/m renal cancer cells with butyrolactone 1.butyrolactone 1 (bl) is a cyclin dependent kinase (cdk) inhibitor derived from aspergillus terreus. none of the present drugs are effective for the treatment of renal cell carcinoma. the use of bl is expected to promote a new type therapy of renal cancer.200312757604
modification of rapid susceptibility assay for antifungal susceptibility testing of aspergillus fumigatus.to improve objectivity and speed of current antifungal mold susceptibility testing, the yeast rapid susceptibility assay (rsa) was adapted for aspergillus species. the rsa is based on glucose utilization in the presence of an antifungal drug. aspergillus fumigatus conidia were incubated in 0.2% glucose rpmi 1640 containing 0.03 to 16 micro g of amphotericin b or itraconazole/ml. drug-related inhibition of glucose utilization correlated with suppression of conidial germination. following incubati ...200312958253
comparison of the sensititre yeastone colorimetric antifungal panel and etest with the nccls m38-a method to determine the activity of amphotericin b and itraconazole against clinical isolates of aspergillus spp.the evaluation of the sensititre yeastone and etest methods for determining susceptibility to amphotericin b and itraconazole by comparing the mics obtained by these methods at different times of reading with those of the m38-a broth microdilution method.200312917236
experimental pulmonary aspergillosis due to aspergillus terreus: pathogenesis and treatment of an emerging fungal pathogen resistant to amphotericin b.aspergillus terreus is an uncommon but emerging fungal pathogen, which causes lethal infections that are often refractory to amphotericin b (amb). in comparison to aspergillus fumigatus, a. terreus was resistant to the in vitro fungicidal effects of safely achievable concentrations of amb. these in vitro findings correlated directly with resistance of a. terreus to amb in experimental invasive pulmonary aspergillosis. residual fungal pulmonary burden and galactomannan antigenemia demonstrated pe ...200312854088
studies in the biochemistry of micro-organisms. 97. flavipin, a crystalline metabolite of aspergillus flavipes (bainier & sartory) thom & church and aspergillus terreus thom. 195613341894
biosynthesis of itaconic acid in aspergillus terreus. ii. early stages in glucose dissimilation and the role of citrate. 195713438854
biosynthesis of itaconic acid in aspergillus terreus. iii. the properties and reaction mechanism of cis-aconitic acid decarboxylase. 195713438855
transformations of cyclic nonaketides by aspergillus terreus mutants blocked for lovastatin biosynthesis at the lova and lovc genes.two mutants of aspergillus terreus with either the lovc or lova genes disrupted were examined for their ability to transform nonaketides into lovastatin 1, a cholesterol-lowering drug. the lovc disruptant was able to efficiently convert dihydromonacolin l 5 or monacolin j 9 into 1, and could also transform desmethylmonacolin j 15 into compactin 3. in contrast, the lova mutant has an unexpectedly active beta-oxidation system and gives only small amounts of 1 upon addition of the immediate precurs ...200312929390
biosynthesis of itaconic acid in aspergillus terreus. i. tracer studies with c14-labeled substrates. 195713438853
onychomycosis due to aspergillus terreus thom. 195212985151
itaconicoxidase: an enzyme from an ultraviolet-induced mutant of aspergillus terreus. 195813590057
biosynthesis of itaconic acid by aspergillus terreus. 196213988524
the formation of cis aconitic decarboxylase in culture of aspergillus terreus. 195914429861
ultraviolet-induced mutational changes in enzyme activity of aspergillus terreus. 195913794487
catabolism of itaconic acid by preformed mats of aspergillus terreus. 196213911957
in vitro activities of three licensed antifungal agents against spanish clinical isolates of aspergillus spp.the aim of the present study was to identify retrospectively trends in the species distributions and the susceptibility patterns of aspergillus species causing fungal infections in spanish medical centers from 2000 to 2002. the susceptibilities of 338 isolates to amphotericin b, itraconazole, and voriconazole were tested. aspergillus fumigatus was the most common species (54.7%), followed by aspergillus terreus (14.8%) and aspergillus flavus (13.9%). non-a. fumigatus species were encountered in ...200314506013
reversal by pyruvate of fluoride inhibition in aspergillus terreus. 196214461673
[analysis of the mycelium of aspergillus terreus]. 196114461603
isolation, purification, and properties of cellulases from aspergillus terreus and penicillium variabile. 196514282936
the mechanism of itaconic acid formation by aspergillus terreus. 2. the effect of substrates and inhibitors. 195514363198
the mechanism of itaconic acid formation by aspergillus terreus. 1. the effect of acidity. 195514363197
aspergillus terreus peritonitis in a capd patient: report of a case. 200415104352
pyrene and benzo(a)pyrene metabolism by an aspergillus terreus strain isolated from a polycylic aromatic hydrocarbons polluted soil.a strain of aspergillus terreus was isolated from a polycyclic aromatic hydrocarbons (pahs) polluted soil. the metabolism of pyrene and benzo(a)pyrene by this fungus was investigated in liquid submerged culture added of 50 and 25 ppm respectively of each compound. depletion of pyrene and benzo(a)pyrene was evident during the first stages of growth and was 60% and 27.5% respectively of the added amount after nine days of culture. solvent extracts of the fermentation broth and mycelium were analys ...200415068369
biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi.the biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and seven fungi species, including imperfect strains and higher level lignolitic species, is compared in a 90-day laboratory experiment using a natural, not-fertilized soil contaminated with 10% crude oil. the natural microbial soil assemblage isolated from an urban forest area was unable to significantly degrade crude oil, whereas pure fungi cultures effectively reduced the residues by 26-35% in 90 days. normal ...199615093496
[a case of pulmonary aspergillosis caused by aspergillus terreus]. 200315004970
subcutaneous infection due to aspergillus terreus.a case of subcutaneous infection by aspergillus terreus is reported. pus from the lesion contained spherules which did not appear to be related to the aspergillus. the nature of the spherules is discussed.196414159452
[biodegradation of herbicide pendimethalin by fungi and its characteristics].fungi, which can grow on and degrade pendimethalin, were isolated from activated sludge and soil. the biodegradation characterization in vitro was studied. the results showed that pendimethalin could be degraded by fungi, 16 fungi were isolated by enrichment culture, among which, 10 fungi could degrade more than 60% pendimethalin in 5 days in culture fluid. according to the physiological resistance and the capacity to degrade pendimethalin, three fungi were considered as efficiently degrading st ...200314997649
crude oil and hydrocarbon-degrading strains of rhodococcus rhodochrous isolated from soil and marine environments in kuwait.soil and marine samples collected from different localities in kuwait were screened for microorganisms capable of oil degradation. both fungi and bacteria were isolated. the fungal flora consisted of aspergillus terreus, a. sulphureus, mucor globosus, fusarium sp. and penicillum citrinum. mucor globosus was the most active oil degrading fungus isolated. bacterial isolates included bacillus spp. enterobacteriaceae, pseudomonas spp., nocardia spp., streptomyces spp.,and rhodococcus spp. among thes ...199015092275
infections due to aspergillus terreus: a multicenter retrospective analysis of 83 cases.current in vitro and in vivo data indicate that invasive aspergillosis due to aspergillus terreus is resistant to treatment with amphotericin b. because little clinical data are available to guide therapy, we performed a retrospective cohort study of cases of invasive a. terreus infections from 1997-2002 to determine whether the use of voriconazole, compared with use of other antifungal therapies, led to an improved patient outcome. we analyzed a total of 83 cases of proven or probable invasive ...200415307028
mechanism of lethal and mutagenic action of ionizing radiation on aspergillus terreus. i. relationship of relative biological efficiency to ion density. 195214927691
the effect of supplementary factors on the radiation-induced frequency of mutations in aspergillus terreus. 195214927687
in vitro evaluation of double and triple combinations of antifungal drugs against aspergillus fumigatus and aspergillus terreus.microdilution broth checkerboard techniques based on the national committee for clinical laboratory standards methodology were used to study double and triple antifungal combinations against clinical isolates of aspergillus fumigatus and a. terreus. the influences of the end-point definition (partial or complete inhibition) and the mode of reading (visually or spectrophotometrically) were determined. interactions between antifungal drugs were also evaluated by agar diffusion tests. combinations ...200414982791
lovastatin inhibits its own synthesis in aspergillus terreus.lovastatin suppresses its own synthesis in the microfungus aspergillus terreus. the inhibitory effect was documented by spiking identical batch cultures with pure lovastatin (0, 50, 100 and 250 mg/l) 24 h after initiation from spores.200414740252
mechanism of lethal and mutagenic action of ionizing radiations on aspergillus terreus. ii. use of modifying agents and conditions. 195214927692
disseminated aspergillus terreus infection arising from cutaneous inoculation treated with caspofungin.a case of disseminated aspergillus terreus infection in a patient with prolonged neutropenia after stem cell transplant for myeloma is reported. the isolate was resistant to amphotericin b in vitro, and the patient was successfully managed with surgical debridement and the recently licensed antifungal agent caspofungin. there are many challenges associated with treating invasive aspergillosis, particularly that due to a. terreus, and the early use of caspofungin should be considered.200314686991
aspergillus terreus: an emerging amphotericin b-resistant opportunistic mold in patients with hematologic malignancies.invasive aspergillosis (ia) has emerged as a common cause of morbidity and mortality among immunocompromised patients. at the university of texas m. d. anderson cancer center (houston, tx), aspergillus terreus is second to a. fumigatus as the most common cause of ia. in the current study, the authors compared the risk factors and outcomes associated with ia caused by a. terreus and ia caused by a. fumigatus.200415378491
role of some fermentation parameters on cyclosporin a production by a new isolate of aspergillus terreus.a local isolate of aspergillus terreus was selected among different microorganisms as a new cyclosporin a (cy a) producing culture. the formation of cy a was investigated under different fermentation conditions (including selection of the cultivation medium, fermentation time course, inoculum nature, medium volume, agitation rate, ph value). relatively high cy a productivities were maintained when the fermentation process was carried out using a medium composed of (g/l): glucose, 50; bactopepton ...200314747973
cytotoxic constituents of aspergillus terreus from the rhizosphere of opuntia versicolor of the sonoran desert.a novel cyclopentenedione, asterredione (1), two new terrecyclic acid a derivatives, (+)-5(6)-dihydro-6-methoxyterrecyclic acid a (2) and (+)-5(6)-dihydro-6-hydroxyterrecyclic acid a (3), and five known compounds, (+)-terrecyclic acid a (4), (-)-quadrone (5), betulinan a (6), asterriquinone d (7), and asterriquinone c-1 (8), were isolated from aspergillus terreus occurring in the rhizosphere of opuntia versicolor, using bioassay-guided fractionation. acid-catalyzed reaction of 2 under mild condi ...200314695798
epidemiology of aspergillus terreus at a university hospital.invasive fungal infections due to aspergillus species have become a major cause of morbidity and mortality among immunocompromised patients. aspergillus terreus, a less common pathogen, appears to be an emerging cause of infection at our institution, the university of alabama hospital in birmingham. we therefore investigated the epidemiology of a. terreus over the past 6 years by using culture data; antifungal susceptibility testing with amphotericin b, voriconazole, and itraconazole; and molecu ...200314662934
microbial transformation of terreusinone, an ultraviolet-a (uv-a) protecting dipyrroloquinone, by streptomyces sp.biotransformation study was conducted on the marine dipyrroloquinone, terreusinone (1) isolated from the marine-derived fungus aspergillus terreus. preparative-scale fermentation of terreusinone with streptomyces sp. has resulted in the isolation of a new oxidized metabolite, terreusinol (2). the structure was elucidated as 2-[(1r)-1-hydroxyisobutyl]-6-[(1r)-1,2-dihydroxyisobutyl]-1h,5h-pyrrolo[2,3-b]indole-4,8-dione (2) on the basis of physicochemical evidence. terreusinol (2) showed an ultravi ...200314646333
[mycosis in the ear, nose and throat].recent trends of fungal infections of the ear, nose and throat were introduced from the viewpoint of otolaryngologic practice. aspergillus terreus was the most common pathogen of otomycosis followed by a. niger and a. flavus. lanoconazole showed the most effective antifungal function for these aspergillus species by drug sensitivity test. biological differences between clinical and soil-borne strains of a. terreus were evaluated. the clinical strains showed slower growth-rate on malt extract aga ...200314615794
occurrence of fructosyl-amino acid oxidase-reactive compounds in fungal cells.fructosyl-amino acid oxidase (faod)-reactive fraction (fry) was found in commercial yeast extract. fry showed very hydrophilic property and was adsorbed to phenylboronate silica gel, indicating that it contained the amadori compound. tlc and amino acid analyses revealed that glucosone, lysine, and arginine were produced from fry after incubation with faod. tof-ms analysis confirmed that fry is a mixture of fructosyl lysine and fructosyl arginine. these compounds were also detected in mycelial ex ...200515665502
fungal infection of sutureless self-sealing incision for cataract surgery.to report the clinical picture and outcome of fungal infection of self-sealing wounds in cataract surgery.200314597526
receptor assay-guided isolation of anti-gabaergic insecticidal alkaloids from a fungal culture.the gamma-aminobutyric acid (gaba) receptor bears sites of action for insecticides. to discover gaba receptor-directed insecticides in natural products, fungal culture extracts were screened for their ability to inhibit specific binding of the radiolabeled noncompetitive antagonist [3h]1-(4-ethynylphenyl)-4-n-propyl-2,6,7-trioxabicyclo[2.2.2]octane to housefly head membranes. the screening efforts led to the isolation of two alkaloids from aspergillus terreus: pf1198a (alantrypinone) and pf1198b ...200415186111
intracellular expression of vitreoscilla hemoglobin in aspergillus terreus to alleviate the effect of a short break in aeration during culture.a continuous supply of o(2) is important for itaconic acid production in aspergillus terreus. any interruption of aeration significantly reduces itaconic acid production. to overcome this effect, a. terreus m8 was transformed with the vitreoscilla hemoglobin gene (vgb) which, as shown by southern hybridization, was integrated into the recipient chromosome. the activity of the expressed hemoglobin was confirmed by a co-difference spectrum. during itaconic acid production, the effect of a break in ...200415218381
nature and mode of action of nad and atp dephosphorylating enzyme from aspergillus terreus dsm 826.nad and atp were dephosphorylated by aspergillus terreus extracts optimally at ph 8 and 40 degrees c. the data obtained indicate that one phosphohydrolase was involved in the cleavage of all the phosphate linkages of these two energy-carrying molecules, and also indicate that this enzyme can be classified as a non-specific alkaline phosphatase. this is based on the following criteria: during fractionation of the enzymes of the extracts, using sephadex g-200 column chromatography, the recorded el ...200314574102
rational elimination of aspergillus terreus sulochrin production.elimination of undesirable co-metabolites from industrial fermentations is often required due to the toxicities associated with the contaminants and/or due to difficulties in removing the contaminants during downstream processing. sulochrin is a co-metabolite produced during the aspergillus terreus lovastatin fermentation. examination of the sulochrin biosynthetic pathway identifies the emodin anthrone polyketide synthase (pks) at the origin. thus, genetically disrupting the emodin anthrone pks ...200415129726
aristolochene synthase: mechanistic analysis of active site residues by site-directed mutagenesis.incubation of farnesyl diphosphate (1) with penicillium roqueforti aristolochene synthase yielded (+)-aristolochene (4), accompanied by minor quantities of the proposed intermediate (s)-(-)germacrene a (2) and the side-product (-)-valencene (5) in a 94:4:2 ratio. by contrast, the closely related aristolochene synthase from aspergillus terreus cyclized farnesyl diphosphate only to (+)-aristolochene (4). site-directed mutagenesis of amino acid residues in two highly conserved mg(2+)-binding domain ...200415186158
synthesis and biological evaluation of analogues of butyrolactone i and molecular model of its interaction with cdk2.a series of analogues of butyrolactone i, a natural product isolated from aspergillus terreus that selectively inhibits the cdk2 and cdk1 kinases and that has been found to exhibit an interesting antiproliferative activity, have been synthesized. its antitumor activity has been tested. molecular models of the complex between butyrolactone i and the cdk2 active site have been built using a combination of conformational search and automated docking techniques. the stability of the resulting comple ...200415227538
production of polyclonal antibodies against territrem b and detection of territrem b in the conidia of aspergillus terreus 23-1 by immunoelectron microscopy.territrem b, a fungal metabolite isolated from aspergillums terreus 23-1, is a tremorgenic mycotoxin. immunoelectron microscopy using anti-territrem b polyclonal antibody was used to detect territrem b in the fungal body of a. terreus 23-1 at different times of culture without shaking on potato dextrose (pd) agar medium. the anti-territrem b serum was produced by immunization of rabbits with 4beta-hydroxymethyl-4beta-demethylterritrem b-sccinate bound by a linker to keyhole limpet hemocyanin. th ...200415161198
a multicenter, open-label clinical study of micafungin (fk463) in the treatment of deep-seated mycosis in japan.the efficacy and safety of micafungin (fk463), which is a new lipopeptide antifungal agent of the echinocandin class and is active against both aspergillus and candida species, were investigated in patients with deep-seated mycosis in this study. 70 patients were treated with micafungin 12.5-150 mg/d intravenously for up to 56 d. the overall clinical response rates were 60% (6/10) in invasive pulmonary aspergillosis, 67% (6/9) in chronic necrotizing pulmonary aspergillosis, 55% (12/22) in pulmon ...200415287383
4-hydroxykigelin and 6-demethylkigelin, root growth promoters, produced by aspergillus terreus.root growth promoters, 4-hydroxykigelin (1) and 6-demethylkigelin (2), together with 6-hydroxymellein (3) were isolated from cultures of the fungus aspergillus terreus and their structures were identified by spectroscopic analysis. the biological activities of the three dihydroisocoumarins, 1, 2, and 3, have been examined using a bioassay method with lettuce seedlings. furthermore, interactions between the dihydroisocoumarins and indole-3-acetic acid against the root growth have been examined.200415241930
aspergillus endocarditis presenting as femoral artery embolism.fungal valvular endocarditis is an unusual cause of endocarditis, yet very important because of its historically poor prognosis. we report two fatal cases of fungal valvular endocarditis following cardiovascular surgery, presenting as femoral artery embolism. aspergillus terreus and a. flavus were the causative agents of endocarditis in the two patients. diagnosis was established very early by culture of the emboli and was confirmed later by isolation of the same aspergillus species from the res ...200415189195
[invasive infection by aspergillus terreus treated successfully with liposomal amphotericin b].a woman of 75 years old was admitted at our hospital for evaluation of worsening and weakness in inferior limbs. several vertebral fractures by crushing, one doubtful discitis, an infiltrate with cavitation in the right superior lobe and one infiltrate in the left superior lobe were detected. in the biopsy of the consolidation a filamentous fungus was watched and in the bronchial washing specimen culture grew aspergillus terreus. the infiltrates disappeared with liposomal amphotericin b remainin ...200415195481
comparison of the sensititre yeastone colorimetric antifungal panel with a modified nccls m38-a method to determine the activity of voriconazole against clinical isolates of aspergillus spp.the susceptibilities of 63 isolates of aspergillus spp. to voriconazole were evaluated by a modified nccls m38-a method and the sensititre yeastone method. the overall agreement was 82.5%, ranging from 100% for aspergillus niger and aspergillus terreus to 62.5% for aspergillus flavus. discrepancies between the methods were due to higher sensititre mics. the sensititre yeastone method could have potential value for susceptibility testing of aspergillus spp. to voriconazole.200415365044
antifungal therapy of murine aspergillus terreus infection.aspergillus terreus is a species which is being seen increasingly frequently and which is highly resistant to amphotericin b in vitro and clinically. we evaluated amphotericin b, caspofungin, and posaconazole in a murine model of acute invasive aspergillosis. caspofungin and posaconazole both appeared beneficial and may be reasonable treatment alternatives for infection with a. terreus.200415388425
serotonin (5-ht) enhances the activity of amphotericin b against aspergillus fumigatus in vitro.we investigated the in vitro synergistic antifungal potential of combining serotonin (5-ht) and sertraline with amphotericin b and itraconazole against clinical isolates of aspergillus spp. synergy tests were performed using the chequerboard microdilution method. activity was measured against aspergillus fumigatus (n = 7), aspergillus flavus (n = 3) and aspergillus terreus (n = 2), and compared with that for candida albicans and candida parapsilosis. the fractional inhibitory concentration (fic) ...200415380269
rapid differentiation of aspergillus species from other medically important opportunistic molds and yeasts by pcr-enzyme immunoassay.we developed a pcr-based assay to differentiate medically important species of aspergillus from one another and from other opportunistic molds and yeasts by employing universal, fungus-specific primers and dna probes in an enzyme immunoassay format (pcr-eia). oligonucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal dna from aspergillus flavus, aspergillus fumigatus, aspergillus nidulans, aspergillus niger, aspergillus terreus, aspergillus ustus, and aspergillus v ...200415297489
in vitro analyses, animal models, and 60 clinical cases of invasive aspergillus terreus infection. 200415328076
fractionation of the naringinase complex from aspergillus terreus by dye affinity chromatography.affinity chromatography with immobilised triazine dyes was used to separate the main enzymes present in the naringinase complex produced by aspergillus terreus cect 2663. one alpha-l-rhamnosidase and two beta-d-glucosidases (beta g1 and beta g2) were separated by a simple two-step procedure involving chromatography with red he-3b immobilised on sepharose 4b first at ph 5.5 and then at ph 4.7. maximum activity of the beta-d-glucosidases was from ph 4 to 6 and at 65 degrees c. both glucosidases we ...200415483384
invasive fungal infections in kuwait: a retrospective study.a retrospective review of a five year period (1994-1998) revealed that opportunistic mycoses caused by ubiquitous fungal pathogens are a serious problem in the immunocompromised patient population of kuwait. patients with renal transplantation and diabetes mellitus were most susceptible to aspergillosis, cryptococcosis, and zygomycosis, whereas patients with candidemia/hematogenous candidiasis had multiple risk factors. basidiobolomycosis of the rectum in a bangladeshi male, cryptococcosis due t ...200015597675
production of l-asparaginase by filamentous fungi.l-asparaginase production was investigated in the filamentous fungi aspergillus tamarii and aspergillus terreus. the fungi were cultivated in medium containing different nitrogen sources. a. terreus showed the highest l-asparaginase (activity) production level (58 u/l) when cultivated in a 2% proline medium. both fungi presented the lowest level of l-asparaginase production in the presence of glutamine and urea as nitrogen sources. these results suggest that l-asparaginase production by of filam ...200415543411
determination of antifungal drug susceptibilities of aspergillus species by a fluorescence-based microplate assay.we have investigated the use of a viability dye, chloromethylfluorescein di-acetate (cmfda), for antifungal susceptibility testing in a fluorescence microplate (fm) assay format.200515546970
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