TitleAbstractYear(sorted ascending)
emodin o-methyltransferase from aspergillus terreus.emodin o-methyltransferase, an enzyme catalyzing methylation of the 8-hydroxy group of emodin, was identified in the mould aspergillus terreus imi 16043, a (+)-geodin producing strain. the enzyme catalyzed the formation of questin from emodin and s-adenosyl-l-methionine. by chromatography on deae-cellulose, phenyl sepharose, q-sepharose, hydroxyapatite, and cm-cellulose, emodin o-methyltransferase was purified to apparent homogeneity. the purified protein had a molecular weight of 322 kda as est ...19921444712
purification and general properties of xylanase from aspergillus terreus.aspergillus terreus thom produced appreciable yield of xylanase on medium containing acid pretreated rice straw as sole carbon source. the enzyme was purified approximately 25-fold by ammonium sulfate precipitation, gel filtration through sephadex g-50 and ion-exchange chromatography on deae-cellulose with a yield of about 23% and specific activity of 15.38 units/mg protein. optimum activity against xylan was at 45 degrees c and ph 4.5. relative stability of the enzyme was recorded at ph range o ...19921471443
isolation of an aspergillus terreus mutant impaired in arginine biosynthesis and its complementation with the argb gene from aspergillus nidulans.using filtration enrichment techniques, an aspergillus terreus arginine auxotrophic strain which contains a mutation that abolishes ornithine transcarbamylase (otcase) activity has been isolated. this mutant has been genetically transformed with the cloned aspergillus nidulans otcase gene. prototrophic transformants arose at a frequency of about 50 transformants per microgram of plasmid dna. southern blot analysis of dna from the transformants showed that the transforming dna was ectopically int ...19921490598
disseminated aspergillosis in a dog with diskospondylitis and neurologic deficits.a german shepherd dog was treated initially for signs of urinary tract infection; subsequently, signs of spinal pain and neurologic deficits developed. fungal hyphae were found in the urine sediment, and spinal radiography revealed changes in the vertebrae and intervertebral disks at the levels of t3 to t8, t12 to t13, l3-4, and l5-6, consistent with diskospondylitis. fungal cultures of urine and specimens from spinal lesions yielded aspergillus terreus. itraconazole (5 mg/kg of body weight, po, ...19921559889
allergic bronchopulmonary aspergillosis caused by aspergillus terreus presenting lobar collapse.a case of allergic bronchopulmonary aspergillosis (abpa) is described which presents left upper lobe collapse caused by aspergillus terreus, an organism rarely found in abpa. it is unusual for initial radiographic findings of abpa to include extensive collapse.19921568035
high performance liquid chromatography of spermidine and spermine using a postcolumn reactor of immobilized polyamine oxidase (aspergillus terreus) followed by electrochemical detection.a highly sensitive and selective method for the determination of spermidine and spermine has been developed. a polyamine oxidase (aspergillus terreus) immobilized column was used as a postcolumn reactor. the detection limit was 0.2 pmol/injection for both spermidine and spermine with a linear range of three orders of magnitude.19921600367
effects of territrem-b on cholinergic responses of snail neuron.effects of territrem-b (trb), a mycotoxin isolated from a rice culture of aspergillus terreus, on the central neuron of the snail achatina fulica were studied electrophysiologically. territrem-b potentiated the acetylcholine (ach) induced current of the neuron, while it had no effect on gaba or l-glutamate elicited currents. trb and neostigmine increased the peak amplitude of the response elicited by the first perfusion of ach and depressed the increase in current produced by a second perfusion. ...19938100056
[invasive bronchopulmonary aspergillosis treated with itraconazole in a patient with acute leukemia].a case of invasive broncopulmonar aspergillosis caused by aspergillus terreus successfully treated with itraconazole is reported in a patient undergoing autologous bone marrow transplantation for acute lymphoblastic leukaemia. although the patient was on prophylaxis with fluconazole and she did not respond to amphotericin b, there was an excellent response to itraconazole which allowed the transplant without any aspergillus infection during both the transplant and the post-transplant periods. du ...19938140504
riboflavin production by aspergillus terreus from beet-molasses.aspergillus terreus was used for riboflavin (vitamin b2) production in a medium containing beet molasses as the sole carbon source. growth and the vitamin production of the fungus were markedly affected by the composition of the culture medium. a maximum riboflavin yield was achieved at the late exponential growth phase (16 day-old cultures) in the presence of (g/l): centrifuged beet-molasses, 90; l-asparagine, 1; mgso4.7h2o, 0.5; k2hpo4/kh2po4 (1:1), 5; and the medium initially adjusted to ph 8 ...19938172689
protein-enrichment of wheat bran using aspergillus terreus.wheat bran was fermented by aspergillus terreus to increase the protein content for use as animal feed. maximum protein content (55%) and conversion efficiency (59%) were achieved at the late growth phase (8 day-old cultures), when each flask containing 100 ml medium was inoculated with 4% (v/v) spore suspension (3.6 x 10(5) spore/ml) and shaken at 250 rpm. the best fermentation medium contained (g/l): wheat bran, 10; urea, 1.4; mgso4.7h2o, 0.3; kh2po4, 1.0; kc1, 0.1 and was adjusted to ph 4.0. ...19938172690
a new nmr airlift bioreactor used in 31p-nmr studies of itaconic acid producing aspergillus airlift bioreactor for in-vivo nmr studies of cells is described. the 10-mm diameter airlift reactor was constructed for studies of mycelial/pellet forming organisms, grown in suspension. with this device 161 mhz 31p-nmr spectra of living aspergillus terreus cells, producing itaconic acid, have been obtained. signals were observed for intra- and extracellular orthophosphate, glycerol-3-phosphorylethanolamine (gpe), glycerol-3-phosphorylcholine (gpc), sugar phosphates and polyphosphate. the sp ...19938227940
aspergillus terreus postoperative endophthalmitis. 19938318491
overexpression in escherichia coli of soluble aristolochene synthase from penicillium roqueforti.aristolochene synthase, a fungal cyclase which has been isolated from aspergillus terreus and penicillium roqueforti, catalyzes the cyclization of farnesyl diphosphate to the sesquiterpene hydrocarbon aristolochene. the aristolochene synthase gene (ari1) of p. roqueforti has previously been cloned and expressed at low levels as a protein a-aristolochene synthase fusion protein in escherichia coli. we have now used the polymerase chain reaction to amplify the aristolochene synthase coding sequenc ...19938346917
aspergillus terreus during hospital renovation. 19938354865
successful treatment of simultaneous pulmonary pseudallescheria boydii and aspergillus terreus infection with oral itraconazole.pseudallescheria boydii and aspergillus terreus are unusual opportunistic fungal pathogens that are often resistant to chemotherapy with amphotericin b. we report a case of simultaneous invasive pulmonary infection with these organisms occurring in a bone marrow transplant recipient who was successfully treated with the oral azole itraconazole.19938392389
fatal disseminated infection with aspergillus terreus in immunocompromised hosts.aspergillus terreus is widespread in the environment but only uncommonly infects humans. it can cause cutaneous and subcutaneous infections, and several cases of local invasive disease have been reported; to our knowledge, however, only five cases of disseminated disease have been documented previously. we describe here four additional patients with disseminated disease caused by a. terreus. all four had an underlying hematologic or lymphoreticular malignancy, were immunocompromised secondary to ...19938448286
nosocomial invasive aspergillosis in lymphoma patients treated with bone marrow or peripheral stem cell determine the prevalence of aspergillosis in lymphoma patients housed in a protective environment while undergoing a bone marrow transplant or peripheral stem cell transplant and its relation to lymphoma type, type of transplant, period of neutropenia, method of diagnosis, species of aspergillus, and the use of empiric amphotericin b.19938478525
production of phenolic compounds by aspergillus s-4 in sake cake medium and identification of terrein.high performance liquid chromatography (hplc) analysis of the culture filtrate of an isolated mold, aspergillus terreus s-4, showed a larger peak of a terrein-like substance than those of gallic acid and protocatechuic acid. the chemical structure of this substance was investigated and identified as terrein, 4,5-dihydroxy-3-propenyl- 2-cyclopenten-1-one. we also examined some culture conditions for the production of terrein using a sake cake medium for strain s-4.199327281008
electrophoretic free mobility and viability of microbial cells: a preliminary study in preparation for space experiments.electrophoretic free mobilities (efm) of four fungal spores and five bacterial cells were determined in 7 mm triethanolamine/acetate (tea) buffer by means of microscopic electrophoresis (me) and free flow electrophoresis (ffe). spores of aspergillus terreus, penicillium citrinum, gliocladium virens, and rhizopus oryzae had similar efm from 2.2 to 3.1 microns sec-1/v cm-1. the resolution of the spore mixture by ffe was therefore determined to be poor. bacterial cells of salmonella typhimurium lt2 ...19947880880
a fatal infection with alternaria alternata and aspergillus terreus in a child with agranulocytosis of unknown origin.alternaria alternata and aspergillus terreus were isolated from cutaneous nodules in a 5-year-old girl with agranulocytosis of unknown origin. histopathological examination supported the diagnosis of an infection with two opportunistic moulds. aspergillus terreus was also isolated from the secretions of the maxillary sinuses of the patient. in spite of antimycotic therapy, the child eventually died from respiratory failure.19947898514
superoxide anion production in response to bacterial lipopolysaccharide and fungal spores implicated in organic dust toxic syndrome.high amounts of fungal spores, bacteria, and bacterial endotoxin have been found in dust associated with the poorly characterized syndrome, organic dust toxic syndrome (odts). as part of an ongoing investigation to determine the etiopathogenesis for odts, this study has focused on activation of guinea pig bronchial alveolar lavage (bal) cells as evidenced by the production of superoxide anion in response to fungal spores and lipopolysaccharide (lps). fungal spores from aspergillus candidus, aspe ...19947925197
general primer-mediated pcr for detection of aspergillus species.a pcr assay was developed for the diagnosis of invasive aspergillosis in immunocompromised patients. for this purpose, the complete nucleotide sequences of the genes encoding the 18s rrna of aspergillus nidulans, aspergillus terreus, aspergillus niger, and aspergillus flavus were elucidated and aligned to the sequences of aspergillus fumigatus and other clinically relevant prokaryotic and eukaryotic microorganisms. genus-specific sequences could be identified in the v7 to v9 region of 18s rrna. ...19947929762
blasticidin s deaminase gene from aspergillus terreus (bsd): a new drug resistance gene for transfection of mammalian cells.blasticidin s deaminase (bsd) is a drug inactivating enzyme produced by aspergillus terreus, which convert blasticidin s (bs) to a non-toxic deamino-hydroxy derivative. the bsd gene was fused to sv 40 transcriptional regulatory elements and the resulting vector was used to transfect fm3a cells. expression of bsd conferred resistance to bs and allowed efficient isolation of integrative transfectants which have stably maintained the bs-resistance phenotype after repeated transfer to fresh selectiv ...19947948022
four new metabolites of aspergillus terreus.four new metabolites (1-4) were isolated from mycelium of aspergillus terreus ifo 6123 producing asterriquinone (arq). the structures of 1 and 2 were shown to be 3,6-bis[1-(1,1-dimethyl-2-propenyl)-1h-indol-3-yl]-furo[3,2-b]furan- 2,5-dione (asterridinone) and 2,5-bis[1-(1,1-dimethyl-2-propenyl)-1h-indol-3- yl]-3-acetoxy-6-hydroxy-2,5-cyclohexadiene-1,4-dione (arq monoacetate), respectively, by the chemical and spectral data. compounds 3 and 4 were identified with known asterriquinone isomers, 2 ...19947954921
the changing spectrum of fungal keratitis in south review the clinical experience with fungal keratitis in south florida over a 10-year period.19948008340
effect of aspergillus terreus extract on neoplastic and non-neoplastic cell replication.there are very few and unconfirmed data regarding the antineoplastic activity of mycotic derivates in human cells. the effects on neoplastic and non-neoplastic cell replication of aspergillus terreus extracts have been tested. in fact, among different species of aspergillus mycotoxin producers. a terreus seems to be more suitable for hypothetical therapeutic purposes because of its low mycotoxin toxicity. very evident antiblastic activity of alcoholic crude extract of a. terreus on tumor cells h ...19948071682
cloning of the blasticidin s deaminase gene (bsd) from aspergillus terreus and its use as a selectable marker for schizosaccharomyces pombe and pyricularia oryzae.aspergillus terreus produces a unique enzyme, blasticidin s deaminase, which catalyzes the deamination of blasticidin s (bs), and in consequence confers high resistance to the antibiotic. a cdna clone derived from the structural gene for bs deaminase (bsd) was isolated by transforming escherichia coli with an aspergillus cdna expression library and directly selecting for the ability to grow in the presence of the antibiotic. the complete nucleotide sequence of bsd was determined and proved to co ...19948159161
[the evaluation of antigens and antisera for their use in the serodiagnosis of aspergillosis].aspergillosis became an important opportunistic mycosis during the last years, with a great variety of clinical manifestations. to contribute to the replenishing of this mycosis serodiagnosis, biologic reactives (antigens and antisera) were prepared from strains of the species aspergillus niger, aspergillus flavus and aspergillus terreus for their use in immunoprecipitation assays. the reactives were assessed by double immunospreading versus a reference commercial system; satisfactory results we ...19959805081
molecular cloning and transcriptional analysis of the aspergillus terreus gla1 gene encoding a glucoamylase.the aspergillus terreus gla1 gene, coding for a glucoamylase, has been cloned by heterologous hybridization. the gene is interrupted by four introns and encodes a protein with an n-terminal catalytic domain and a c-terminal starch-binding domain. the expression of the gene is induced by starch and maltose and repressed by glucose.19957534054
aspergillus terreus, an uncommon fungus causing aortic root abscess and pseudoaneurysm.aspergillus terreus is a rare fungus which can cause opportunistic infection in humans. a fifteen year old girl had undergone open aortic valvotomy for aortic stenosis and post-stenotic dilatation of the ascending aorta. she was discharged after an uneventful post-operative period. she was reoperated later for para-aortic abscess. the pus on culture yielded pure growth of aspergillus terreus from multiple media. to our knowledge, this is the first report from india of aspergillus terreus causing ...19957558098
effect of aerobic pretreatment with aspergillus terreus on the anaerobic digestion of olive-mill wastewater.a kinetic study was carried out on the anaerobic digestion of olive-mill wastewater (omw) and omw that was previously fermented with aspergillus terreus. the bioreactors used were batch fed and contained saponite as support for the mediating bacteria. the anaerobic digestion process followed first-order kinetics, from which the kinetic constant a was calculated using a non-linear regression. this kinetic parameter was influenced by the pretreatment carried out, and was 3.7 times higher for pretr ...19957576261
disseminated opportunistic fungal disease in dogs: 10 cases (1982-1990).medical records of 10 dogs in which fungal infection was diagnosed between 1982 and 1990 were reviewed. in each dog, infection was determined to be caused by a single species of fungus, either aspergillus terreus, penicillium sp, paecilomyces sp, chrysosporium sp, or pseudallescheria boydii. nine dogs were german shepherd dogs; 1 was a german shepherd dog cross, and 9 were females. the most common clinical signs were signs of neck or back pain (9 dogs), weight loss (7 dogs), anorexia (6 dogs), p ...19957601696
effects of aspergillus terreus extract on herpes simplex 1 virus this study we reported about the antiviral activity of aspergillus terreus alcohol extract against herpes simplex type 1 virus. this activity is dose-dependent, is related to the concentration of the challenging virus and depends particularly on a proteic fraction of 5-10 kda. optimal effects were observed with doses ranging from 25 to 6.25 micrograms/ml for crude alcohol extract and up to 3 micrograms/ml for 5-10 kda fraction. moreover, antiviral activity was evident in viral replication, bu ...19957629556
molecular cloning and heterologous expression of the gene encoding dihydrogeodin oxidase, a multicopper blue enzyme from aspergillus terreus.aspergillus terreus dihydrogeodin oxidase (dhgo) is an enzyme catalyzing the stereospecific phenol oxidative coupling reaction converting dihydrogeodin to (+)- geodin. we previously reported the purification of dhgo from a. terreus and raised polyclonal antibody against dhgo. from the first cdna library constructed in lambda gt11 using mrna from 3-day-old mycelium of a. terreus, four clones were identified using anti-dhgo antibody, but all contained partial cdna inserts around 280 base pairs. th ...19957665560
acetylcholinesterase inhibition by territrem b derivatives.five derivatives of territrem b [2], a potent acetylcholinesterase inhibitor isolated from a rice culture of aspergillus terreus, were prepared from 2 as well as from its major metabolite, 4 beta-hydroxymethyl-4 beta-demethylterritrem b [4]. the inhibitory activities of these derivatives against electric eel acetylcholinesterase (e.c. were tested and it was concluded that the enone and the pyrone groups present in 2 may play an important biological role.19957673929
production of beta-glucosidase by aspergillus terreus.the production of beta-glucosidase by aspergillus terreus was investigated in liquid shake cultures. enzyme production was maximum on the 7th day of growth (2.18 u/ml) with the initial ph of the medium in the range of 4.0-5.5. cellulose (sigmacell type 100) at 1.0% (wt/vol) gave maximum beta-glucosidase activity among the various soluble and insoluble carbon sources tested. potassium nitrate was a suitable nitrogen source for enzyme production. triton x-100 at 0.15% (vol/vol) increased the enzym ...19957766152
itaconate biosynthesis in aspergillus terreus.itaconate biosynthesis was studied in intact cells of high-yield (rc4') and low-yield (cm85j) strains of the fungus aspergillus terreus by methods (tracers, nuclear magnetic resonance spectroscopy, and mass spectroscopy) that did not interfere with metabolism. itaconate formation in rc4' required de novo protein biosynthesis. krebs cycle intermediates increased in both strains during the production of itaconic acid. the embden-meyerhof-parnas pathway and the krebs cycle were shown to be involved ...19957768868
properties of enzymes involved in d-galactonate catabolism in fungi.two enzymes catalyze the two step reactions in the d-galactonate nonphosphorolytic catabolic pathway of aspergillus terreus, namely d-galactonate dehydratase and 2-keto-3-deoxy-d-galactonate (kdgal) aldolase. maximum enzyme activities were obtained at 40 degrees c and ph 8.0 or at 50 degrees c and ph 7.5 for these two enzymes, respectively. stability of the two enzymes under different conditions was investigated. from a lineweaver-burk plot of the reciprocal of initial velocities and substrate c ...19957771768
efficacy of oral saperconazole in systemic murine aspergillosis.saperconazole is a fluorinated bis-triazole. groups of ten 5-week-old female cd-1 mice were infected intravenously with 5.5 x 10(7) aspergillus conidia. saperconazole, dissolved in hydroxypropyl-beta-cyclodextrin (hpbc), was given orally twice a day for 11 days, beginning 1 day post-infection, at 50, 100 or 200 mg kg-1 day-1. at day 18 post-infection, survivors were killed and residual infection quantified in the kidneys. with aspergillus fumigatus isolate 10af, 70% given no therapy, 100% given ...19958544084
chronic bilateral suppurative otitis media caused by aspergillus terreus.a case of bilateral suppurative otitis media is described in a patient with the presenting symptoms of otorrhoea, itching, mild deafness, heaviness in the ear and otalgia. the patient had perforation in the tympanic membrane. histological sections revealed aggregated fungal masses of aspergillus terreus. the infection responded well to topical ketoconazole therapy. incidentally, this is the second case from india of chronic suppurative otitis media caused by fungi.19958559193
molecular probes for diagnosis of fungal infections.we have developed 21 specific nucleic acid probes which target the large subunit rrna genes from aspergillus flavus, aspergillus fumigatus, aspergillus glaucus, aspergillus niger, aspergillus terreus, blastomyces dermatitidis, candida albicans, candida (torulopsis) glabrata, candida guilliermondii, candida kefyr, candida krusei, candida lusitaniae, candida parapsilosis, candida tropicalis, coccidioides immitis, cryptococcus neoformans var. gattii, cryptococcus neoformans var. neoformans, filobas ...19958576345
long-term survival of four dogs with disseminated aspergillus terreus infection treated with itraconazole.four dogs with disseminated aspergillosis caused by aspergillus terreus were treated with oral itraconazole for 190 to 1095 days. infection was eliminated in 1 dog. two dogs were treated for 1000 and 1095 days but were eventually euthanased 572 and 485 days after treatment was stopped. at necropsy both dogs had widespread aspergillosis. the fourth dog was euthanased for other reasons after 190 days of treatment when it was showing a good clinical response although there was radiographic evidence ...19958579564
blasticidin s deaminase gene (bsd): a new selection marker gene for transformation of arabidopsis thaliana and nicotiana tabacum.arabidopsis thaliana and nicotiana tabacum were transformed to blasticidin s (bs) resistance with bsd (the bs deaminase gene from aspergillus terreus) using the agrobacterium-mediated transformation method. expression of bsd allowed direct selection of transformants by the fungicide, and both kinds of transgenic plants showed high level of resistance phenotype at 100 ppm of bs sprayed on the leaves. using botrytis cinerea, the causal fungus of gray mold disease, it was exemplified that applicati ...19958611760
diagnostic utility of thoracentesis for neutropenic children with cancer.thoracentesis is a procedure often performed in children with pleural effusions to assist in diagnosis and management. its safety and utility for immunocompromised patients with neutropenia (absolute neutrophil count, <1,500 polymorphonuclear leukocytes and band forms per microl) is unclear. we reviewed our experience over a 10-year period to evaluate the role of thoracentesis for neutropenic children with cancer who had pulmonary effusions of presumed infectious etiology. twenty-two patients we ...19958645835
[mutation site-specific assay (mssa) for the detection of hepatitis b virus precore defective mutant in hepatitis b infection]. 199512442404
biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and pure cultures of imperfect and lignolitic fungi.the biodegradation of aliphatic and aromatic hydrocarbons by natural soil microflora and seven fungi species, including imperfect strains and higher level lignolitic species, is compared in a 90-day laboratory experiment using a natural, not-fertilized soil contaminated with 10% crude oil. the natural microbial soil assemblage isolated from an urban forest area was unable to significantly degrade crude oil, whereas pure fungi cultures effectively reduced the residues by 26-35% in 90 days. normal ...199615093496
the cu,zn superoxide dismutases of aspergillus flavus, aspergillus niger, aspergillus nidulans, and aspergillus terreus: purification and biochemical comparison with the aspergillus fumigatus cu,zn superoxide,zn superoxide dismutases (sods) have been purified to homogeneity from aspergillus flavus and a. niger, which are significant causative agents of aspergillosis, and from a. nidulans and a. terreus, which are much rarer causative agents of disease, using a combination of isoelectric focusing and gel filtration fast protein liquid chromatography. the purified enzymes have been compared with the previously described sod from the most important pathogen in the genus, a. fumigatus (m. d. holdom, r ...19968757871
isozyme polymorphism of cellulases in aspergillus terreus.maximum cellulase production in aspergillus terreus was obtained at a temperature of 28 degrees c, ph 4.0 and a substrate concentration of 1% cmc. variability in cellulase enzyme production and isozyme polymorphism of endo-beta-1,4-glucanase and beta-1,4-glucosidase was studied in 45 natural isolates of a. terreus. different electrophoretic patterns were evident for endoglucanase. three zones of activity viz eg 1, eg 11 and eg 111 were observed showing different electrophoretic mobilities. some ...19968765085
[invasive mold infections in the university clinics of leipzig in the period from 1992-1994].invasive mould infection, e. g. aspergillosis in the first place, is a common infection in immunocompromised patients. the diagnosis of invasive mould infection is difficult in the absence of confirmation by tissue biopsy and histological studies. therefore, prevalence of invasive mould infections at the school of medicine of the leipzig university between 1992 and 1994 was investigated. the diagnosis of invasive mould infection was suspected on clinical, mycological, and radiological findings. ...19968767281
comparative fungicidal activity of a new quaternary ammonium salt, n-alkyl-n-2-hydroxyethyl-n,n-dimethylammonium butyl phosphate and commonly used disinfectants.we synthesized a new quaternary ammonium salt, n-alkyl-n-2-hydroxyethyl-n, n-dimethylammonium butyl phosphate (abp) that does not precipitate in the presence of anionic surfactants by incorporating a paired butyl phosphate anion into cationic surfactants. abp showed much greater bactericidal activities and antirusting effects than benzalkonium chloride (bac). in this study, the fungicidal effects of abp were evaluated in comparison with common disinfectants [bac, chlorhexidine digluconate (chx) ...19968850330
molecular structure of terrecyclodiol: a derivative of the antifungal metabolite terrecyclic acid a from aspergillus terreus.a strain of aspergillus terreus, which was isolated from organic mulch and inhibited the growth of the plant pathogen phytophthora cinnamomi, produces an antifungal metabolite when grown in liquid culture. this metabolite was isolated by bioassay-guided fractionation and identified as terrecyclic acid a (1). x-ray diffraction studies and spectroscopic details of the derived terrecyclodiol (2) are described.19968984154
cloning of the polyketide synthase gene atx from aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression.southern blot analysis of genomic dnas of several fungi that produce polyketide compounds with the 6-methylsalicylic acid synthase (msas) gene of penicillium patulum as a probe indicated the presence of an msas-homologous gene in the (+)-geodin-producing strain imi 16,043 of aspergillus terreus. the gene, designated atx was cloned from an a. terreus genomic dna library and 7588 bp of the gene together with its flanking regions were sequenced to reveal the presence of a 5.5 kb open reading frame ...19969003280
primary structures of fungal fructosyl amino acid oxidases and their application to the measurement of glycated proteins.fructosyl amino acid oxidase (faod), which is active toward model compounds of the glycated proteins in blood, n epsilon-fructosyl n sigma-z-lysine and n-fructosyl valine, was purified to homogeneity from aspergillus terreus gp1. though the enzyme did not use glycated proteins directly as its substrate, it used glycated human serum albumin (hsa) when hsa was treated with a protease. linear relationships between both the concentration and the increase in absorbance and the glycation rate of glyca ...19969022674
multifocal aspergillus terreus discospondylitis in two german shepherd dogs.multifocal fungal (aspergillus terreus) discospondylitis was diagnosed in 2 german shepherd dogs. in one dog, the aetiology was established by means of fluoroscopic-guided disc aspiration, cytology and culture of disc material and urine. disseminated aspergillosis was confirmed at necropsy and a. terreus cultured from numerous organs in this dog. the aetiology in the other dog was not established until therapeutic failure forced surgical curettage of disc material from which the fungus was cultu ...19969284036
nondermatophyte causes of onychomycosis and superficial mycoses.compared to dermatophytes, nondermatophytes that may cause distal and lateral subungual onychomycoses are aspergillus species, acremonium species, fusarium oxysporum and scopulariopsis brevicaulis. white superficial onychomycosis may be caused by nondermatophyte species, for example, acremonium species, aspergillus terreus, other aspergillus species and fusarium oxysporum. nondermatophyte molds such as scopulariopsis brevicaulis may uncommonly result in cutaneous infections. scytalidium dimidiat ...19969504061
putative diels-alder-catalyzed cyclization during the biosynthesis of lovastatin.a diels-alder cyclization proposed to occur during polyketide synthase assembly of the bicyclic core of lovastatin (1) (mevinolin) by aspergillus terreus mf 4845 was examined via the synthesis of the n-acetylcysteamine (nac) thioester of [2,11-(13)c(2)]-(e,e,e)-(r)-6-methyldodecatri-2,8,10-enoate (5a). in vitro diels-alder cyclization of the corresponding unlabeled nac ester 5b, ethyl ester 18b, and acid 20b yielded two analogous diastereomers in each case, under either thermal or lewis acid-cat ...199611667090
territrems: naturally occurring specific irreversible inhibitors of acetylcholinesterase.territrem b (trb), a fungal metabolite isolated from aspergillus terreus, potently and noncompetitively inhibited electrophorus acetylcholinesterase (ache ec, but had no inhibitory effect on horse serum butyrylcholinesterase (btche ec the trb-treated ache did not recover its enzyme activity after either dialysis or dilution of the inhibited enzyme. the binding of [(14)c]trb to ache, but not to btche, was demonstrated. the concentrations of territrems required for 50% inhibitio ...199611725083
[a case of allergic bronchopulmonary aspergillosis caused by aspergillus terreus].a 66-year-old woman was admitted to our hospital with a cough, wheezing, and expectoration. chest x-ray and ct scanning revealed atelectasis and infiltration of the middle lobe, but no central bronchiectatic change. the patient had eosinophilic infiltration elevated serum ige, rast against aspergillus )(a.) fumigatus, a positive immediate skin reaction, and a positive test for antibodies against a. funmigatus. bronchoscopy demonstrated mucoid impaction that plugged the middle lobe bronchus. the ...19979567092
mechanism of the cytotoxicity of asterriquinone, a metabolite of aspergillus terreus.asterriquinone (arq) is an antitumor metabolite of aspergillus terreus ifo 6123. arq is a quinone compound and the mechanism of its antitumor action was compared with that of its analog arqdme which was dimethylated at the dihydroxy benzoquinone moiety, and adriamycin (adr). the cytotoxicity of arq against p388 cells was less than that of adr, and arqdme showed hardly any cytotoxicity. arq and adr formed dna-interstrand cross links (isc) in p388 cells in a concentration while the isc index by ar ...19979413222
polyketide synthase gene pksm from aspergillus terreus expressed during growth phase.the polyketide synthase gene pksm was detected in the genomic dna library of aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-msas) gene of penicillium patulum as a probe. 9524 bp of the cloned dna were sequenced and a 5.5 kb open reading frame was revealed. a single intron (62 bp) was identified in the conserved position. two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation ini ...19979438344
mevinolin production by some fungi.the potentiality of twenty-five fungal species belonging to 14 genera isolated from egyptian soils to produce mevinolin, a hypocholesterolemic agent, when grown on selected substrates was tested. for the first screening samples of culture filtrates were tested by tlc and the positive results were further estimated by hplc analysis. it was found that nearly one-third of the tested fungi showed positive results as to production of mevinolin. aspergillus terreus was distinguished by its capacity to ...19979438350
articular aspergillosis: two case reports and review of the literature.we report two cases of septic arthritis of the knee caused by aspergillus: one by aspergillus terreus in a cirrhotic patient and the other by aspergillus fumigatus after vascular graft infection. the recovery of these organisms in synovial fluid should be considered as pathogenic, particularly in immunocompromised hosts. the pathogenic mechanism is discussed and the literature is reviewed.19979448597
detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes.aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ...19979458383
the phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi aspergillus terreus and myceliophthora thermophila.phytases catalyse the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate. in this study genes encoding novel phytases from two different filamentous fungi, aspergillus terreus strain 9a-1 and myceliophthora thermophila were isolated. the encoded phya phytase proteins show 60% (a. terreus) and 48% (m. thermophila) identity, respectively, to the phya of aspergillus niger and have 21-29% identity compared to other histidine acid phosphatases. all three phy ...19979025298
a retrospective analysis of microbial contaminants in outdated random-donor platelets from multiple sites.platelet components contaminated with bacteria are an important source of transfusion-associated bacterial sepsis. estimates of contamination rates vary widely (0-10%) and are highly controversial. the present study, designed with stringent testing regimens, retrospectively determined the prevalence of microbial contaminants in platelets from four collection regions.19979122897
cloning and molecular analysis of the aspergillus terreus arg1 gene coding for an ornithine aspergillus terreus gene (arg1) encoding ornithine carbamoyltransferase (octase) has been cloned and sequenced. the deduced amino acid sequence contains a signal peptide suggestive of a mitochondrial location for the a. terreus enzyme. alignment of the a. terreus octase sequence with other octases revealed the presence of conserved regions. northern analysis indicates that arg1 expression is regulated at the level of transcription and that transcription of the arg1 gene is not markedly affect ...19979141661
chemical and functional properties of mutastein, an inhibitor of insoluble glucan synthesis by streptococcus sobrinus.mutastein, a potent inhibitor of insoluble glucan synthesis by streptococcus sobrinus, is a protein with a molecular weight of approximately 2 x 10(6). amino acid and elisa analyses suggested that mutastein is a mixture of heterogenous polymers of alpha-casein contained in the culture medium of the producing strain, aspergillus terreus m3328. mutastein strongly inhibited the primer-dependent insoluble glucan synthase of s. sobrinus b13. the primer-independent soluble glucan synthase was not affe ...19979145515
in vitro activity of sch-56592 and comparison with activities of amphotericin b and itraconazole against aspergillus this study, we investigated the in vitro activity of sch-56592 (sch), a new triazole antifungal agent. we compared the activity of sch with those of itraconazole (itz) and amphotericin b (ab) against 60 clinical isolates of aspergillus spp. by using a microtiter format. incubation was done at 37 degrees c for 48 h, and mic endpoints (no growth) were read visually. the medium used for all of the drugs was rpmi 1640 buffered with morpholinepropanesulfonic acid (mops) and supplemented with 2% gl ...19979145880
immunoreactivity of a 38-kilodalton penicillium marneffei antigen with human immunodeficiency virus-positive sera.penicillium marneffei produced and secreted a 38-kda antigen that appeared to be specific for this dimorphic fungus. this component could not be detected in antigenic extracts of histoplasma capsulatum, cryptococcus neoformans, aspergillus niger, aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, and two other species of penicillium by immunoblot analysis against the sera from patients with culture-confirmed penicilliosis marneffei. antibody reactive with this anti ...19979276391
concentration- and time-dependent upregulation and release of the cytokines mip-2, kc, tnf, and mip-1alpha in rat alveolar macrophages by fungal spores implicated in airway inflammation.inhalation of fungal spores has been shown to cause primary or secondary infection and respiratory inflammation and diseases such as allergic alveolitis, atopic asthma, and organic dust toxic syndrome, which are rarely reported in the absence of predisposing factors. biochemical and molecular markers of inflammation were measured in rat bronchial alveolar lavage cells (> 95% macrophages) following stimulation with fungal spores isolated from pathogenic and nonpathogenic fungi that have been impl ...19989490662
invasive pulmonary aspergillosis due to aspergillus terreus: 12-year experience and review of the literature.a 12-year retrospective analysis was done to identify and evaluate in detail cases of invasive pulmonary aspergillosis (ipa) caused by aspergillus terreus. we identified 13 a. terreus infections among 133 total cases of confirmed invasive aspergillosis; 11 were ipa and 2 were primary peritoneal infections. of the 11 patients with ipa, 7 developed neutropenia during hospitalization, and the remaining four were receiving immunosuppressive agents. ten patients with ipa died; one liver transplantati ...19989597234
comparison of in vitro activities of the new triazole sch56592 and the echinocandins mk-0991 (l-743,872) and ly303366 against opportunistic filamentous and dimorphic fungi and yeasts.the in vitro antifungal activities of sch56592, mk-0991, and ly303366 against 83 isolates of acremonium strictum, aspergillus flavus, aspergillus fumigatus, aspergillus terreus, bipolaris spp., blastomyces dermatitidis, cladophialophora bantiana, fusarium oxysporum, fusarium solani, histoplasma capsulatum, phialophora spp., pseudallescheria boydii, rhizopus arrhizus, scedosporium prolificans, and sporothrix schenckii were compared. the in vitro activities of these agents against 104 isolates of ...19989738049
effect of butyrolactone i on the producing fungus, aspergillus terreus.butyrolactone i [alpha-oxo-beta-(p-hydroxyphenyl)-gamma-(p-hydroxy-m-3, 3-dimethylallyl-benzyl)-gamma-methoxycarbonyl-gamma-butyrolactone] is produced as a secondary metabolite by aspergillus terreus. because small butyrolactone-containing molecules act as self-regulating factors in some bacteria, the effects of butyrolactone i on the producing organism were studied; specifically, changes in morphology, sporulation, and secondary metabolism were studied. threefold or greater increases in hyphal ...19989758788
endocarditis and aortal embolization caused by aspergillus terreus in a patient with acute lymphoblastic leukemia in remission: diagnosis by peripheral-blood culture.disseminated infection with aspergillus terreus is a rare disease that affects only the immunocompromised host. we report a case of systemic infection with a. terreus resulting in endocarditis, aortic embolization, and splenic infarction in a patient with acute lymphoblastic leukemia. diagnosis through peripheral blood culture, lack of pulmonary involvement, and onset of disease during complete remission from leukemia constitute uncommon features of this case.19989774591
relationship between the structure and cytotoxic activity of asterriquinone, an antitumor metabolite of aspergillus terreus, and its alkyl ether derivatives.asterriquinone (arq) is an antitumor metabolite of aspergillus terreus ifo 6123. in this study we synthesized several arq alkyl ethers and studied their cytotoxic activity against mouse leukemia p388 cells. the dissociation constant was also similar among the arq monoalkyl ethers. there was a good correlation between the hydrophobicity of arq monoalkyl ethers estimated by high performance liquid chromatography and the intracellular content accumulated for 60 min. arq monoalkyl ethers were cytoto ...19989781844
purification and characterization of a regiospecific lipase from aspergillus terreus.aspergillus terreus lipase was purified to homogeneity with 18.0% yield. the specific activity of the enzyme increased from 20.80 to 250 u/mg of protein. ion exchange on q-sepharose was highly effective in the purification process. the molecular mass of the purified enzyme was 41+/-1 kda as determined by sds/page. the purified lipase showed excellent temperature tolerance (15-90 degreesc) and was highly thermostable, retaining 100% activity at 60 degreesc for 24 h. it showed good ph tolerance (3 ...19989799723
in-vitro testing of susceptibility to amphotericin b is a reliable predictor of clinical outcome in invasive aspergillosis.invasive aspergillosis is a life-threatening fungal infection which, in neutropenic patients, is associated with an extremely high mortality rate despite optimal treatment. in order to investigate microbiological risk factors for treatment failures in more detail, aspergillus spp. obtained from 29 patients with haematological diseases after myelo-ablative chemotherapy and bone marrow transplantation were analysed for their susceptibility to amphotericin b in vitro and this was compared with clin ...19989818749
fungal endocarditis in bone marrow transplantation: case report and review of literature.we report a case of fungal endocarditis due to aspergillus terreus in a leukaemia patient who received a bone marrow allograft from a matched unrelated donor. he presented with persistent fever. microangiopathic haemolytic anaemia but there was no cardiac signs and symptoms. he died despite intravenous amphotericin b. review of the english literature showed five other patients with fungal endocarditis in the setting of bone marrow transplantation and revealed the same features of difficult diagn ...19989892534
availability of phosphorus and sulfur of insecticide origin by fungi.thirteen fungal species isolated from soil treated with pesticides were tested for their ability to mineralize and degrade three organophosphate insecticides currently used in egypt (cyolan, malathion and dursban) in liquid media free from phosphorus (p) and sulfur (s). all fungal species grew successfully on the culture media treated with the three used doses of insecticides (10, 50 and 100 ppm active ingredient) but the growth rate varied with the species, the insecticide and the doses. at 10 ...199810192894
inactivation of blasticidin s by bacillus cereus. vi. structure and comparison of the bsr gene from a blasticidin s-resistant bacillus cereus.two types of recombinant plasmids containing 600 bp nde i fragments that coded the bsr gene in opposite directions were obtained. nucleotide sequencing shows that the bsr encodes a 140 amino acid protein with a putative molecular weight of 15560, the same as that of purified blasticidin s (bs)-deaminase (bsr), on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) (15500). upstream of the open reading frame, a shine-dalgarno (sd) sequence, frequent inverted repeats, and the sigm ...199810607416
aspergillus terreus and its toxic metabolites as a food contaminant in some egyptian bakery products and grains.a. terreus isolates isolated from some bakery products, corn and rice were found to be able to produce territrems. 90% of thea. terreus isolated from bakery products were able to produce territrem a, with a mean of 0.09 ppm, while 80% ofa. terreus isolates produce territrem b with a mean of 0.24 ppm. on the other hand 31.8% of the isolates ofa. terreus from corn were able to produce territrem a with a mean of 0.44 ppm. concerninga. terreus isolates from rice, 66.7% were found to produce territre ...199823605064
molecular typing of aspergillus terreus by random amplification of polymorphic dna. 199910614966
use of the 6-methylsalicylic-acid-synthase gene as a discriminating marker between aspergillus terreus and aspergillus nineteen pathogenic and saprophytic isolates denoted as aspergillus terreus the presence and restriction pattern of the gene pksm (6-methylsalicylic acid synthase) was determined. five patterns (a-e) were found and in three isolates the gene was missing. the rapd (random amplified polymorphic dna) patterns with three primers were analyzed. the strains with pksm pattern possibly derived from the most common pattern a by single mutation (patterns b-d) were also related by rapd. among them, thre ...199910997132
comparison of e-test and broth microdilution methods for antifungal drug susceptibility testing of molds.we compared the e test with a broth microdilution method, performed according to national committee for clinical laboratory standards document m27-a guidelines, for determining the in vitro susceptibilities of 90 isolates of pathogenic molds (10 absidia corymbifera, 10 aspergillus flavus, 10 aspergillus fumigatus, 10 aspergillus niger, 10 aspergillus terreus, 10 exophiala dermatitidis, 10 fusarium solani, 10 scedosporium apiospermum, 5 scedosporium prolificans, and 5 scopulariopsis brevicaulis). ...199910203509
correlation between fungi isolated from burn wounds and burn care units.a comparison was made prospectively between fungal isolates from patients and burn care units. aspergillus niger was the most frequent isolate in both patients and burn care units whereas ulocladium was the commonest isolate in the control group. aspergillus terreus, penicillium and zygomycetes, which were recovered from burned patients, were also found more frequently in the burn care units than in the control group (other areas in the hospital). these findings indicate a potential risk of fung ...199910208389
comparison of in vitro activity of liposomal nystatin against aspergillus species with those of nystatin, amphotericin b (ab) deoxycholate, ab colloidal dispersion, liposomal ab, ab lipid complex, and itraconazole.we compared the in vitro activity of liposomal nystatin (nyotran) with those of other antifungal agents against 60 aspergillus isolates. twelve isolates were itraconazole resistant. for all isolates, geometric mean (gm) mics (micrograms per milliliter) were 2.30 for liposomal nystatin, 0.58 for itraconazole, 0.86 for amphotericin b (ab) deoxycholate, 9.51 for nystatin, 2.07 for liposomal ab, 2.57 for ab lipid complex, and 0.86 for ab colloidal dispersion. aspergillus terreus (gm, 8.72 micrograms ...199910223948
biodegradation of fluoranthene by soil fungi.a selection of 39 strains of micromycetes known as good degraders of polychlorinated aromatic compounds, mostly isolated from soil and belonging to various taxonomic groups, have been investigated for fluoranthene degradation. toxicity assays, first evaluated on solid medium mea, have not shown any toxicity of fluoranthene (1-100 mg.l-1) towards fungi. whereas, consumption assays on a solid synthetic medium showed a toxicity at 100 mg.l-1. the degradation of fluoranthene (10 mg.l-1) was then inv ...199910230046
in vitro amphotericin b resistance in clinical isolates of aspergillus terreus, with a head-to-head comparison to voriconazole.amphotericin b therapy continues to be the "gold standard" in the treatment of invasive aspergillosis in the immunocompromised host. although aspergillus fumigatus and aspergillus flavus constitute the major species, several reports have described invasive pulmonary or disseminated disease due to the less common aspergillus terreus and dismal clinical outcomes with high-dose amphotericin b. we therefore evaluated 101 clinical isolates of a. terreus for their susceptibility to amphotericin b and ...199910364610
lovastatin biosynthesis in aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene.lovastatin, an hmg-coa reductase inhibitor produced by the fungus aspergillus terreus, is composed of two polyketide chains. one is a nonaketide that undergoes cyclization to a hexahydronaphthalene ring system and the other is a simple diketide, 2-methylbutyrate. fungal polyketide synthase (pks) systems are of great interest and their genetic manipulation should lead to novel compounds.199910381407
a set of independent selectable markers for transfection of the human malaria parasite plasmodium falciparum.genomic information is rapidly accumulating for the human malaria pathogen, plasmodium falciparum. our ability to perform genetic manipulations to understand plasmodium gene function is limited. dihydrofolate reductase is the only selectable marker presently available for transfection of p. falciparum. additional markers are needed for complementation and for expression of mutated forms of essential genes. we tested parasite sensitivity to different drugs for which selectable markers are availab ...199910411941
bronchocentric granulomatosis due to aspergillus terreus in an immunocompetent and non-asthmatic woman.this is the first report of bronchocentric granulomatosis due to aspergillus terreus in a healthy and non-asthmatic 74-year-old japanese woman. following identification of the fungus, oral itraconazole therapy was begun after intrabronchial infusion of amphotericin b. no recurrence has occurred after treatment for 24 months. we should consider the possibility of bronchocentric granulomatosis including aspergillus terreus, when an intrabronchial lesion is found even in a healthy and non-asthmatic ...199910542983
territrem b, a tremorgenic mycotoxin that inhibits acetylcholinesterase with a noncovalent yet irreversible binding mechanism.territrem b (trb) is a fungal metabolite isolated from aspergillus terreus shown previously to be a potent and irreversible inhibitor of acetylcholinesterase (ache). in the present study, a number of binding and inhibition assays were carried out to further characterize the inhibitory effect of trb. the results indicate that the binding of trb (a) is much more selective than a well characterized selective inhibitor of ache, bw284c51, (b) adopts a one-to-one stoichiometry with the enzyme, (c) can ...199910574966
production of aspergillus xylanase by lignocellulosic waste fermentation and its application.strains of aspergillus terreus and a. niger, known to produce xylanase with undetectable amounts of cellulase, were studied for xylanase (ec production on various lignocellulosic substrates using solid state fermentation. of the lignocellulosic substrates used, wheat bran was the best for xylanase production. the effects of various parameters, such as moistening agent, level of initial moisture content, temperature of incubation, inoculum size and incubation time, on xylanase production ...199910583678
in-vitro susceptibility of aspergillus spp. isolates to amphotericin b and itraconazole.the mics of amphotericin b and itraconazole for 230 isolates of aspergillus spp., comprising 156 aspergillus fumigatus, 20 aspergillus terreus, 22 aspergillus flavus, 17 aspergillus nidulans and 15 aspergillus niger, were determined by a broth microdilution method with rpmi 1640 medium. no isolate was detected with an mic of amphotericin b >2 mg/l. itraconazole mics >16 mg/l were detected for four aspergillus fumigatus and one aspergillus nidulans isolates.199910588320
biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance.phytases (myo-inositol hexakisphosphate phosphohydrolases) are found naturally in plants and microorganisms, particularly fungi. interest in these enzymes has been stimulated by the fact that phytase supplements increase the availability of phosphorus in pig and poultry feed and thereby reduce environmental pollution due to excess phosphate excretion in areas where there is intensive livestock production. the wild-type phytases from six different fungi, aspergillus niger, aspergillus terreus, as ...19999925554
biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties.supplementation with phytase is an effective way to increase the availability of phosphorus in seed-based animal feed. the biochemical characteristics of an ideal phytase for this application are still largely unknown. to extend the biochemical characterization of wild-type phytases, the catalytic properties of a series of fungal phytases, as well as escherichia coli phytase, were determined. the specific activities of the fungal phytases at 37 degreesc ranged from 23 to 196 u. (mg of protein)-1 ...19999925555
activity of an aspergillus terreus alpha-arabinofuranosidase on phenolic-substituted oligosaccharides.the effect of phenolic substitutions on the activity of an alpha-arabinofuranosidase from aspergillus terreus was investigated using feruloylated oligosaccharides isolated from plant cell walls, equivalent oligosaccharides obtained through treatment with specific ferulic acid esterases, and a synthetic lignin-carbohydrate complex (lcc). feruloyl substituents limited the hydrolysis of arabinoxylan and arabinan oligosaccharides but only if the feruloyl group was esterified to the terminal non-redu ...19999987847
value of different methods for the characterisation of aspergillus terreus evaluate different methods for strain differentiation, 10 isolates of aspergillus terreus from germany and two epidemiologically unrelated strains were investigated. the sources of the isolates were patients with cystic fibrosis (4), immunosuppression (2), otitis externa (2), sinusitis (1) and endocarditis (1). environmental isolates were obtained from a contaminated cell culture and from soil. the isolates did not differ in their macroscopic and microscopic morphology, in their protein patte ...19999989644
crystallization and preliminary x-ray diffraction studies of blasticidin s deaminase from aspergillus terreus.blasticidin s deaminase from aspergillus terreus was crystallized with polyethylene glycol 8000. two types of crystals were grown under the same crystallization conditions. one type grew as thin plates, while the other had a rhombic shape. the rhombic shaped crystal was suitable for high-resolution crystal structure analysis. precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group p212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56 ...199910089374
effect of aspergillus terreus mycotoxins on nitric oxide synthase activity in human erythroid k-562 cells.because several stimuli of microbial origin enhance the activity of nitric oxide synthase (nos) in human cells of the myeloid lineage, we decided to investigate whether cellular damage induced by aspergillus terreus mycotoxins could be associated with an increase in nos activity. a pool of mycotoxins rather than individual toxins was tested so that the natural conditions could be mimicked. in the present study, we report that a crude extract of a. terreus induces cellular damage and increases no ...199910191507
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