humoral immunity in disseminated aspergillus terreus infection in the dog.aspects of humoral immunity were studied in 17 dogs with disseminated aspergillosis (16 cases aspergillus terreus, 1 case aspergillus flavipes). all dogs had markedly raised serum igg levels by single radial immunodiffusion (range 1500-6000 mg dl-1). despite this, serum antibody to a. terreus was demonstrated in only 7/16 cases by agar gel diffusion, 9/16 cases by counter immunoelectrophoresis, 10/16 by elisa and 11/16 by an indirect immunofluorescence assay. serum antibody was also detected in ...19883131949
aspergillus terreus as a cause of invasive pulmonary aspergillosis.a 70-year-old woman with nodular, poorly differentiated lymphocytic lymphoma is the third reported patient with invasive pulmonary aspergillosis caused by aspergillus terreus. this case differs from the two previously reported in that neither neutropenia nor broad spectrum antibiotics preceded the infection. a terreus should not be dismissed as a laboratory contaminant in pulmonary specimens, especially those from immunosuppressed patients.19883168592
[synthesis and secretion of the components of the cellulase complex in aspergillus terreus].aspergillus terreus was found to have two functionally different cellobiases. one of them is excreted into the surrounding medium and is a part of the cellulase complex. the second one is an endocellular (or periplasmic) enzyme. it is distinguished by its stability, the time of mrna operation, the sorption of cell debris; moreover, its activity does not depend on changes in the parameters of the medium when the incubation mixture is replaced and the surface is treated with 0.5 m nacl. the synthe ...19836413830
[endocarditis with unusual causative agents].in the period 1947-1985, 601 patients with infective endocarditis were seen at the university hospital zurich and the kantonsspital lucerne. streptococci, enterococci and staphylococci were the predominant causative organisms in two-thirds of all cases. in more than 25% of the patients blood cultures remained negative. in 6 patients endocarditis was caused by very rare organisms, viz. coxiella burnetii (2 cases), hemophilus parainfluenzae, corynebacterium bovis (diphtheroids), brucella melitensi ...19873324331
aspergillus terreus infection in a patient with chronic lymphocytic leukaemia and polycythaemia rubra vera. 19883362085
chemical structure of a new metabolite isolated from the mycelium of aspergillus terreus var. aureus. 19846529817
studies on the metabolic pathway for itatartaric acid formation by aspergillus terreus. ii. use of (-)citramalate, citraconate and itaconate by cell-free extracts. 19744836927
aspergillosis: comparative virulence, metabolic rate, growth rate and ubiquinone content of soil and human isolates of aspergillus terreus. 19744850653
studies on metabolites produced by aspergillus terreus var. aureus. i. chemical structures and antimicrobial activities of metabolites isolated from culture broth. 19836671258
the biosynthesis of phenols. xxiv. the conversion of the anthraquinone question into the benzophenone, sulochrin, in cultures of aspergillus terreus. 19725065535
[production of l-asparaginase by aspergillus terreus]. 19715151228
citreoviridins from aspergillus terreus. 19806773444
[dermatoonychomycosis caused by aspergillus terreus thom]. 19685304714
effect of l-asparaginase from aspergillus terreus on ascites sarcoma in the rat. 19705411861
[aspergillus terreus as a cause of mycotic abortion in cows].the fungus aspergillus terreus thom et church was subjected to microscopic and cultivation study in the organs of an aborted foetus and was demonstrated to be the causative agent of abortion in cattle. the culture of the isolated organism is described and brief data reporting on its ecology are presented. attention is drawn to the probable source of infection and to the insufficiently known aspects of the pathogenesis of this disease.19816782741
influence of ph on the formation and location of beta-glucosidase in aspergillus terreus grown on cellulose.wild strain of aspergillus terreus is very good producer of beta-glucosidase. it produces 15 nkat/ml (0.9 iu/ml) of extracellular beta-glucosidase at ph 5.0. the medium ph level strongly affects the production and binding of beta-glucosidase on the cells and on residual cellulose. at ph 4.0 the rate of enzyme synthesis and the level of total activity is highest but 60-75% of this activity is bound. at higher ph levels the enzyme is mainly released to the medium.19826815035
in vivo pulmonary response to aspergillus terreus spores.the fate of intratracheally instilled aspergillus terreus spores was followed in both rabbits and rats. phagocytosis of the spores by the pulmonary macrophage was rapid in that approx. 42% of the observed spores were associated with the macrophages immediately after instillation. direct penetration of the lung architecture by the spores was not observed but spores were seen in the alveolar interstitium at 3 hr after instillation and in the tracheobroncheal lymph nodes at 24 hr. granulomas formed ...19836825418
allergic bronchopulmonary aspergillosis caused by aspergillus terreus. 19836830061
[fractionation of the cellulase complex of the fungus aspergillus terreus].a procedure for isolating a complex of extracellular cellulases from the aspergillus terreus culture liquid using sp-sephadex c-50 has been developed. enzymatically and electrophoretically homogeneous preparations of cellobiase i and cellobiase ii have been obtained. by means of gel-filtration their molecular weights have been estimated to be 140,000 and 67,000. cellobiase i consists of five and cellobiase ii of two polypeptide chains. three cellulases with various ratios of endoglucanase and ex ...19836878208
mevinolinic acid biosynthesis by aspergillus terreus and its relationship to fatty acid biosynthesis.mevinolinic acid, the open acid form of mevinolin, which is a metabolite of aspergillus terreus, has been shown to be a competitive inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme a reductase (alberts et al., proc. natl. acad. sci. u.s.a. 77:3957-3961, 1980). the biosynthesis of mevinolinic acid was studied by examining the incorporation of [1-14c]acetate and [methyl-14c]methionine into the molecule. these isotopes were rapidly incorporated into mevinolinic acid, with [1-14c]acetate ...19853988710
mycotic pneumonia and meningoencephalitis due to aspergillus terreus in a neonatal snow leopard (panthera uncia). 19854032629
the itaconic and itatartaric acid formation by uv and gamma irradiated isolates of aspergillus terreus nrrl 1960. 19674166071
effect of methylendioxyphenyl synergists on metabolism of carbaryl by aspergillus terreus. 19744474950
influence of nitrogen source on the synthesis of fat from sucrose by aspergillus terreus, aspergillus ochraceus, cladosporium fulvum, cladosporium herbarum and penicillium gladioli. 19724672593
microbial hydroxylation of 5-anilino-1,2,3,4-thiatriazole.two hundred eighty-five fungi, including 100 basidiomycetes and 35 yeasts, 75 actinomycetes, and 40 bacteria were screened for their ability to convert 5-anilino-1,2,3,4-thiatriazole (at) to 5-(p-hydroxyanilino)-1,2,3,4-thiatriazole (p-ht). eleven cultures were found that formed p-ht, which was isolated and whose structure was determined. aspergillus tamarii nrrl 3280 formed 8.6 g of p-ht/liter from 10 g of at/liter (78.9% conversion) in shaken flasks and 4.57 g of p-ht/liter from 6 g of at/lite ...19734699219
assimilatory and dissimilatory utilization of nitrate by aspergillus terreus mutants. 19705433028
cervical lymphadenopathy caused by aspergillus terreus. 19695774321
the biosynthesis of phenols. 5. the relationships of some phenolic metabolites of mutants of aspergillus terreus thom, i.m.i. 16043. 19645832298
studies on terreic was found that aspergillus sp. no. y-8980 which was isolated from a soil sample collected at yoron island in kagoshima prefecture belonged to aspergillus terreus group by morphological observation. the active substance produced by the strain was obtained with a high yield in sucrose-yeast extract medium and extracted by chloroform, ethyl acetate and n-butanol at ph 2.4 approximately 2.6 from the culture broth. the substance was crystallized from chloroform and ethyl acetate after charcoal tre ...19807190624
the microbiology of spent mushroom compost and its dust.microorganisms in spent steamed mushroom compost and its dust were enumerated, and identified. some phase ii (indoor composting) compost samples were also examined. steaming of spent compost resulted in a 70-76% reduction in microbial numbers. total counts made with compost fusion agar were approximately two logs greater than those for nutrient agar. the most common bacterial isolate was bacillus licheniformis. the most common actinomycete isolates were streptomyces diastaticus and thermoactinom ...19817197578
variations inth the cultural characteristics of rhizoctonia solani, and its antagonists: aspergillus terreus and aspergillus flavus occurring in the rhizosphere of cotton. 19655877017
studies on the metabolic pathway for itatartaric acid formation by aspergillus terreus. i. metabolism of glucose and some c5 and c6-carboxylic acids. 19744836926
cellulolytic activity of moulds. iv. evaluation of the utility of cellulosic wastes for biosynthesis of cellulases and xylanase by aspergillus terreus f-413.the value of various cellulosic wastes for the synthesis of cellulolytic enzymes by the mould aspergillus terreus f-413 was determined in submerged culture. the best results were obtained by using beet pulp. the dynamics of their synthesis was observed during a 12-day culture on the medium most favourable for the synthesis of cellulases.19846083706
mutastein, a new inhibitor of adhesive-insoluble glucan synthesis by glucosyltransferases of streptococcus mutans.mutastein, a new inhibitor of glucosyltransferases (gtase) of streptococcus mutans, was isolated from cultural broth of aspergillus terreus m3328. mutastein was a heat-stable protein and was sensitive to pronase digestion. in the formation of glucans, that of adhesive water-insoluble glucans of s. mutans was specifically inhibited by mutastein.19836219978
aspergillus terreus endocarditis--a case report. 19836368727
a new era of exploitation of microbial the past history of the pharmaceutical industry, secondary metabolites have been screened almost exclusively for antimicrobial activities. this biased and narrow view has severely limited the potential application of microbial metabolites. fortunately, this situation is changing and we are now entering into a new era in which microbial metabolites are being applied to diseases heretofore only subjected to synthetic compounds. this new approach is the application of microbial secondary metabol ...19836400479
aspergillus terreus, an uncommon fungus causing aortic root abscess and pseudoaneurysm.aspergillus terreus is a rare fungus which can cause opportunistic infection in humans. a fifteen year old girl had undergone open aortic valvotomy for aortic stenosis and post-stenotic dilatation of the ascending aorta. she was discharged after an uneventful post-operative period. she was reoperated later for para-aortic abscess. the pus on culture yielded pure growth of aspergillus terreus from multiple media. to our knowledge, this is the first report from india of aspergillus terreus causing ...19957558098
[regulation of the biosynthesis of endo-1,4-beta-glucanase, eco-1,4-beta-glucosidase and cellobiase in aspergillus terreus].this work was aimed at studying how the biosynthesis of endo-1,4-beta-glucanase, exo-1,4-beta-glucosidase and cellobiase, components of the cellulase complex was regulated in an aspergillus terreus strain isolated from soil. the biosynthesis of endoglucanase and cellobiase was suppressed by readily metabolizable substrates and stimulated in the course of growth on lactose and celluloses. the activity of exoglucosidase was found in the medium only when the strain was grown on celluloses. cataboli ...19836405133
disseminated opportunistic fungal disease in dogs: 10 cases (1982-1990).medical records of 10 dogs in which fungal infection was diagnosed between 1982 and 1990 were reviewed. in each dog, infection was determined to be caused by a single species of fungus, either aspergillus terreus, penicillium sp, paecilomyces sp, chrysosporium sp, or pseudallescheria boydii. nine dogs were german shepherd dogs; 1 was a german shepherd dog cross, and 9 were females. the most common clinical signs were signs of neck or back pain (9 dogs), weight loss (7 dogs), anorexia (6 dogs), p ...19957601696
studies on the biosynthesis of terrecyclic acid a, an antitumor antibiotic from aspergillus terreus.the biosynthesis of terrecyclic acid a was investigated using 13c-labeled acetates and mevalonate. 13c nmr spectral analysis of isolated labeled terrecyclic acid demonstrated that the structure is assembled via an isoprene pathway.19846511664
molecular cloning and heterologous expression of the gene encoding dihydrogeodin oxidase, a multicopper blue enzyme from aspergillus terreus.aspergillus terreus dihydrogeodin oxidase (dhgo) is an enzyme catalyzing the stereospecific phenol oxidative coupling reaction converting dihydrogeodin to (+)- geodin. we previously reported the purification of dhgo from a. terreus and raised polyclonal antibody against dhgo. from the first cdna library constructed in lambda gt11 using mrna from 3-day-old mycelium of a. terreus, four clones were identified using anti-dhgo antibody, but all contained partial cdna inserts around 280 base pairs. th ...19957665560
disseminated infection by aspergillus terreus.a patient with acute nonlymphocytic leukemia who received chemotherapy developed lung nodules and later central nervous system symptoms consistent with disseminated aspergillosis. the diagnosis was made at open lung biopsy by culturing the organism and observing in tissue sections conidia borne laterally along the hyphae, a characteristic of the aspergillus terreus-flavipes group. this is the first reported case of disseminated a. terreus infection in an immunocompromised host.19836637885
production of beta-glucosidase by aspergillus terreus.the production of beta-glucosidase by aspergillus terreus was investigated in liquid shake cultures. enzyme production was maximum on the 7th day of growth (2.18 u/ml) with the initial ph of the medium in the range of 4.0-5.5. cellulose (sigmacell type 100) at 1.0% (wt/vol) gave maximum beta-glucosidase activity among the various soluble and insoluble carbon sources tested. potassium nitrate was a suitable nitrogen source for enzyme production. triton x-100 at 0.15% (vol/vol) increased the enzym ...19957766152
itaconate biosynthesis in aspergillus terreus.itaconate biosynthesis was studied in intact cells of high-yield (rc4') and low-yield (cm85j) strains of the fungus aspergillus terreus by methods (tracers, nuclear magnetic resonance spectroscopy, and mass spectroscopy) that did not interfere with metabolism. itaconate formation in rc4' required de novo protein biosynthesis. krebs cycle intermediates increased in both strains during the production of itaconic acid. the embden-meyerhof-parnas pathway and the krebs cycle were shown to be involved ...19957768868
growth and enzyme production by aspergillus terreus in holocellulose.the growth of aspergillus terreus thom in holocellulose medium and the production of xylanase and cellulolytic enzymes were studied and compared with growth and enzyme production in xylan and cellulose powder media. hemicellulose and cellulose were utilized simultaneously by the fungus when grown in holocellulose medium. the growth was similar in the presence of xylan and cellulose powder, but xylan was consumed earlier than cellulose. cellulose powder, carboxymethylcellulase and xylanase activi ...19846721328
electrophoretic free mobility and viability of microbial cells: a preliminary study in preparation for space experiments.electrophoretic free mobilities (efm) of four fungal spores and five bacterial cells were determined in 7 mm triethanolamine/acetate (tea) buffer by means of microscopic electrophoresis (me) and free flow electrophoresis (ffe). spores of aspergillus terreus, penicillium citrinum, gliocladium virens, and rhizopus oryzae had similar efm from 2.2 to 3.1 microns sec-1/v cm-1. the resolution of the spore mixture by ffe was therefore determined to be poor. bacterial cells of salmonella typhimurium lt2 ...19947880880
terrecyclic acid a, a new antibiotic from aspergillus terreus. iii. 13c nmr spectrum of terrecyclic acid a.assignment of the fifteen carbons of terrecyclic acid a, c15h20o3, a new sesquiterpene antibiotic, in the 13c nmr spectrum was performed by 13c-[1h] selective proton decoupling experiments, comparison with spectra of its derivatives and chemical shifts.19846735922
the effects of 2,4-dichlorophenoxyacetic acid on respiration, nitrogen and carbohydrate metabolism of aspergillus terreus thom.the effects of 2,4-d on respiration, carbohydrate and nitrogen metabolism have excited much interest in relation to higher plants (hansen 1946, hseuth and lou 1946, smith et al. 1947, and said and naguib 1955). but the effects of 2,4-d on dungi have been tackled to a much less extent (guiscafre-arrilaga 1948, bever and slife 1948, wei and ling 1948, and manil and strazewska 1950). these investigators studied the effects exerted on fungal growth. said and naguib (1962) studied the effect of 2,4-d ...19806775436
general primer-mediated pcr for detection of aspergillus species.a pcr assay was developed for the diagnosis of invasive aspergillosis in immunocompromised patients. for this purpose, the complete nucleotide sequences of the genes encoding the 18s rrna of aspergillus nidulans, aspergillus terreus, aspergillus niger, and aspergillus flavus were elucidated and aligned to the sequences of aspergillus fumigatus and other clinically relevant prokaryotic and eukaryotic microorganisms. genus-specific sequences could be identified in the v7 to v9 region of 18s rrna. ...19947929762
allergic bronchopulmonary aspergillosis (abpa) caused by aspergillus terreus: specific lymphocyte sensitization and antigen-directed serum opsonic activity.a 16-year-old male presented with a history of asthma and recurrent pneumonia. a diagnosis of abpa was based upon the typical clinical presentation, peripheral eosinophilia, elevated ige and positive immediate skin tests to aspergillus. sputum cultures grew a. terreus, a rare cause of human disease. soluble and particulate antigens were prepared from this organism. precipitins against a. terreus, but not against a. fumigatus, were detected in the patient's serum. his lymphocytes proliferated mar ...19816781385
the effects of mevinolin on serum cholesterol levels of rabbits with endogenous hypercholesterolemia.mevinolin, a fungal metabolite isolated from cultures of aspergillus terreus, is a potent competitive inhibitor of 3-hydroxy-3-methyl glutaryl coenzyme a reductase, the rate-controlling enzyme in cholesterol biosynthesis. in the current studies we demonstrate that mevinolin significantly lowers serum cholesterol in rabbits fed a cholesterol free, low-fat semi-synthetic diet. rabbits maintained on this diet developed endogenous hypercholesterolemia with average cholesterol concentrations of 310 m ...19826921995
the changing spectrum of fungal keratitis in south review the clinical experience with fungal keratitis in south florida over a 10-year period.19948008340
mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme a reductase and a cholesterol-lowering agent.mevinolin, a fungal metabolite, was isolated from cultures of aspergillus terreus. the structure and absolute configuration of mevinolini and its open acid form, mevinolinic acid, were determined by a combination of physical techniques. mevinolin was shown to be 1,2,6,7,8,8a-hexahydro-beta, delta-dihydroxy-2,6-dimethyl-8-(2-methyl-1-oxobutoxy)-1-naphthalene-hepatanoic acid delta-lactone. mevinolin in the hydroxy-acid form, mevinolinic acid, is a potent competitive inhibitor of 3-hydroxy-3-methyl ...19806933445
aspergillus terreus infection of pseudoaneurysm of aortofemoral vascular graft with contiguous vertebral osteomyelitis.this is a case report of a patient who developed several unusual complications of an aortofemoral vascular graft. these were thrombosis, pseudoaneurysm, and infection. there was an aspergillus terreus infection of the pseudoaneurysm of the vascular prosthesis. a contiguous vertebral osteomyelitis due to a. terreus subsequently developed. this represented a localized, invasive form of aspergillosis.19827064922
aspergillus terreus, a pathogen capable of causing infective endocarditis, pulmonary mycetoma, and allergic bronchopulmonary aspergillosis.aspergillus terreus is a rarely reported pathogen in humans, recovery of this organism from the sputum may therefore be discounted as representing contamination this report describes 3 patients--1 with allergic bronchopulmonary aspergillosis (abpa), 1 with aspergilloma, and 1 with infective endocarditis-- in whom a. terreus was proved to be the etiologic organism. together, these 3 cases span the spectrum of disease usually associated with a. fumigatus. detection of serum precipitins against asp ...19827091887
cloning of the blasticidin s deaminase gene (bsd) from aspergillus terreus and its use as a selectable marker for schizosaccharomyces pombe and pyricularia oryzae.aspergillus terreus produces a unique enzyme, blasticidin s deaminase, which catalyzes the deamination of blasticidin s (bs), and in consequence confers high resistance to the antibiotic. a cdna clone derived from the structural gene for bs deaminase (bsd) was isolated by transforming escherichia coli with an aspergillus cdna expression library and directly selecting for the ability to grow in the presence of the antibiotic. the complete nucleotide sequence of bsd was determined and proved to co ...19948159161
[ultrastructural surface restructurings of the fungus aspergillus terreus growing on cellulose-containing substrate].the ultrastructural organization of the cell wall surface was studied in the fungus aspergillus terreus 17p growing on different substrates: glucose, a sodium salt of carboxymethyl cellulose (cmc) which is a soluble derivative of cellulose, ground straw. cells grown on soluble substrates (glucose and na-cmc) had a smooth surface in the logarithmic growth phase. cells grown on straw, a solid insoluble substrate, released a substance of a polysaccharide nature which formed a thick fibrillar networ ...19827121328
protein-enrichment of wheat bran using aspergillus terreus.wheat bran was fermented by aspergillus terreus to increase the protein content for use as animal feed. maximum protein content (55%) and conversion efficiency (59%) were achieved at the late growth phase (8 day-old cultures), when each flask containing 100 ml medium was inoculated with 4% (v/v) spore suspension (3.6 x 10(5) spore/ml) and shaken at 250 rpm. the best fermentation medium contained (g/l): wheat bran, 10; urea, 1.4; mgso4.7h2o, 0.3; kh2po4, 1.0; kc1, 0.1 and was adjusted to ph 4.0. ...19938172690
solvent systems for improved isolation and separation of territrems a and b.territrems a and b, tremorgenic mycotoxins in the rice culture of aspergillus terreus, were extracted with hot chloroform. the toxins were cleaned on a silica gel column by direct adsorption-concentration of the extracts followed by desorption with chloroform-acetone (9:1, vol/vol). crude toxin mixtures were separated by silica gel column chromatography and eluted with benzene-ethyl acetate (65:35, vol/vol). the method gave 112 mg of territrem a, 379 mg of territrem b, and 80 mg of their mixture ...19827149718
antitumor effect and structure-activity relationship of asterriquinone analogs.asterriquinone (arq), a metabolic product from aspergillus terreus ifo 6123, showed an inhibitory effect on some transplantable animal tumors, ehrlich carcinoma, ascites hepatoma ah13 and mouse p388 leukemia, but had little or no effect on yoshida sarcoma, ascites hepatoma ah109a and mouse l1210 leukemia. arq also inhibited the growth of hela cells in monolayer culture. although metabolic products from aspergillus terreus var. africanus ifo 8835 were almost ineffective against ehrlich carcinoma, ...19827152200
overexpression in escherichia coli of soluble aristolochene synthase from penicillium roqueforti.aristolochene synthase, a fungal cyclase which has been isolated from aspergillus terreus and penicillium roqueforti, catalyzes the cyclization of farnesyl diphosphate to the sesquiterpene hydrocarbon aristolochene. the aristolochene synthase gene (ari1) of p. roqueforti has previously been cloned and expressed at low levels as a protein a-aristolochene synthase fusion protein in escherichia coli. we have now used the polymerase chain reaction to amplify the aristolochene synthase coding sequenc ...19938346917
terrecyclic acid a, a new antibiotic from aspergillus terreus. i. taxonomy, production, and chemical and biological properties. 19827174531
terrecyclic acid a, a new antibiotic from aspergillus terreus. ii. structure of terrecyclic acid a. 19827174532
dihydromevinolin, a potent hypocholesterolemic metabolite produced by aspergillus terreus.a new, potent hypocholesterolemic agent is produced by cultures of aspergillus terreus. the isolation of the compound and its characterization as 4a,5-dihydromevinolin containing a trans-fused octahydro-naphthalene system are described. comparative data for dihydromevinolin and mevinolin in three biological assays are given: in vitro inhibition of hmg-coa reductase, inhibition of sterol synthesis in cell cultures, and inhibition of cholesterol synthesis in vivo in rats.19817275832
nosocomial invasive aspergillosis in lymphoma patients treated with bone marrow or peripheral stem cell determine the prevalence of aspergillosis in lymphoma patients housed in a protective environment while undergoing a bone marrow transplant or peripheral stem cell transplant and its relation to lymphoma type, type of transplant, period of neutropenia, method of diagnosis, species of aspergillus, and the use of empiric amphotericin b.19938478525
acute-phase reactants in experimental inhalation lung investigation on the occurrence and role of acute-phase reactants in experimental inhalation lung disease (ild) was undertaken. using an experimental model of ild in which rabbits are exposed to aerosols of appropriate fungal spores, haptoglobin analysis was compared with depressions in arterial oxygen tension (pao2) with time following challenge. haptoglobin values of rabbits exposed to single 30-min aerosol challenges of aspergillus terreus, demonstrated a significant (two- to three fold) i ...19817323066
aspergillus terreus infective endocarditis on a porcine heterograft valve.we present the first reported case of aspergillus endocarditis occurring on a porcine heterograft prosthesis and discuss the literature regarding both aspergillus endocarditis and endocarditis on a porcine valve. a 65-year-old man underwent aortic valve replacement with a porcine heterograft. several weeks later he exhibited signs of infection and then of valve failure. upon removal, the porcine valve substance was found to be grossly infiltrated and destroyed by the aspergillus organism. the po ...19807351851
sem studies on the in vivo uptake of aspergillus terreus spores by alveolar macrophages.pulmonary reactions to fungal spores constitute an important aspect of occupational pneumoconioses. experimental models using aerosols of aspergillus terreus spores produce an acute, complement dependent, hypoxic reaction in rabbits. the purpose of this study was to explore the possible role of the alveolar macrophage in initiating this reaction. rabbits and rats were intratracheally injected with aspergillus spores and the fate of the spores was studied at various times thereafter by light and ...19807414272
long-term survival of four dogs with disseminated aspergillus terreus infection treated with itraconazole.four dogs with disseminated aspergillosis caused by aspergillus terreus were treated with oral itraconazole for 190 to 1095 days. infection was eliminated in 1 dog. two dogs were treated for 1000 and 1095 days but were eventually euthanased 572 and 485 days after treatment was stopped. at necropsy both dogs had widespread aspergillosis. the fourth dog was euthanased for other reasons after 190 days of treatment when it was showing a good clinical response although there was radiographic evidence ...19958579564
isocitrate lyase from aspergillus terreus irrl 16043. 19807429598
molecular cloning and transcriptional analysis of the aspergillus terreus gla1 gene encoding a glucoamylase.the aspergillus terreus gla1 gene, coding for a glucoamylase, has been cloned by heterologous hybridization. the gene is interrupted by four introns and encodes a protein with an n-terminal catalytic domain and a c-terminal starch-binding domain. the expression of the gene is induced by starch and maltose and repressed by glucose.19957534054
the cu,zn superoxide dismutases of aspergillus flavus, aspergillus niger, aspergillus nidulans, and aspergillus terreus: purification and biochemical comparison with the aspergillus fumigatus cu,zn superoxide,zn superoxide dismutases (sods) have been purified to homogeneity from aspergillus flavus and a. niger, which are significant causative agents of aspergillosis, and from a. nidulans and a. terreus, which are much rarer causative agents of disease, using a combination of isoelectric focusing and gel filtration fast protein liquid chromatography. the purified enzymes have been compared with the previously described sod from the most important pathogen in the genus, a. fumigatus (m. d. holdom, r ...19968757871
effect of aerobic pretreatment with aspergillus terreus on the anaerobic digestion of olive-mill wastewater.a kinetic study was carried out on the anaerobic digestion of olive-mill wastewater (omw) and omw that was previously fermented with aspergillus terreus. the bioreactors used were batch fed and contained saponite as support for the mediating bacteria. the anaerobic digestion process followed first-order kinetics, from which the kinetic constant a was calculated using a non-linear regression. this kinetic parameter was influenced by the pretreatment carried out, and was 3.7 times higher for pretr ...19957576261
hypersensitivity pneumonitis concomitant with acute airway obstruction after exposure to hay dust.the case of a non-atopic farmer who had developed severe respiratory as well as systemic symptoms upon exposure to hay dust during the past several years is presented. inhalation challenge by his own hay induced an unusual association of peripheral and central airway obstruction beginning immediately, and hardly susceptible to therapeutic control, as well as the typical hypersensitivity pneumonitis reaction several hours later. by means of double immunodiffusion it was possible to demonstrate se ...19846522842
isolation, chemical structure, acute toxicity, and some physicochemical properties of territrem c from aspergillus terreus.territrem c, a new tremorgenic mycotoxin (c28h32o9; molecular weight, 512.20) was isolated from the chloroform extract of rice cultures of aspergillus terreus 23-1, which also produces territrems a and b. isolation, acute toxicity, and some physicochemical properties of territrem c are discussed in this paper. the spectral and chemical evidence indicated that the structural difference between territrem c and territrem b (c29h34o9) was in their phenyl moieties: a 4-hydroxy-3,5-dimethoxy phenyl gr ...19846546485
molecular structure of terrecyclodiol: a derivative of the antifungal metabolite terrecyclic acid a from aspergillus terreus.a strain of aspergillus terreus, which was isolated from organic mulch and inhibited the growth of the plant pathogen phytophthora cinnamomi, produces an antifungal metabolite when grown in liquid culture. this metabolite was isolated by bioassay-guided fractionation and identified as terrecyclic acid a (1). x-ray diffraction studies and spectroscopic details of the derived terrecyclodiol (2) are described.19968984154
cloning of the polyketide synthase gene atx from aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression.southern blot analysis of genomic dnas of several fungi that produce polyketide compounds with the 6-methylsalicylic acid synthase (msas) gene of penicillium patulum as a probe indicated the presence of an msas-homologous gene in the (+)-geodin-producing strain imi 16,043 of aspergillus terreus. the gene, designated atx was cloned from an a. terreus genomic dna library and 7588 bp of the gene together with its flanking regions were sequenced to reveal the presence of a 5.5 kb open reading frame ...19969003280
primary structures of fungal fructosyl amino acid oxidases and their application to the measurement of glycated proteins.fructosyl amino acid oxidase (faod), which is active toward model compounds of the glycated proteins in blood, n epsilon-fructosyl n sigma-z-lysine and n-fructosyl valine, was purified to homogeneity from aspergillus terreus gp1. though the enzyme did not use glycated proteins directly as its substrate, it used glycated human serum albumin (hsa) when hsa was treated with a protease. linear relationships between both the concentration and the increase in absorbance and the glycation rate of glyca ...19969022674
properties of enzymes involved in d-galactonate catabolism in fungi.two enzymes catalyze the two step reactions in the d-galactonate nonphosphorolytic catabolic pathway of aspergillus terreus, namely d-galactonate dehydratase and 2-keto-3-deoxy-d-galactonate (kdgal) aldolase. maximum enzyme activities were obtained at 40 degrees c and ph 8.0 or at 50 degrees c and ph 7.5 for these two enzymes, respectively. stability of the two enzymes under different conditions was investigated. from a lineweaver-burk plot of the reciprocal of initial velocities and substrate c ...19957771768
a retrospective analysis of microbial contaminants in outdated random-donor platelets from multiple sites.platelet components contaminated with bacteria are an important source of transfusion-associated bacterial sepsis. estimates of contamination rates vary widely (0-10%) and are highly controversial. the present study, designed with stringent testing regimens, retrospectively determined the prevalence of microbial contaminants in platelets from four collection regions.19979122897
cloning and molecular analysis of the aspergillus terreus arg1 gene coding for an ornithine aspergillus terreus gene (arg1) encoding ornithine carbamoyltransferase (octase) has been cloned and sequenced. the deduced amino acid sequence contains a signal peptide suggestive of a mitochondrial location for the a. terreus enzyme. alignment of the a. terreus octase sequence with other octases revealed the presence of conserved regions. northern analysis indicates that arg1 expression is regulated at the level of transcription and that transcription of the arg1 gene is not markedly affect ...19979141661
a fatal infection with alternaria alternata and aspergillus terreus in a child with agranulocytosis of unknown origin.alternaria alternata and aspergillus terreus were isolated from cutaneous nodules in a 5-year-old girl with agranulocytosis of unknown origin. histopathological examination supported the diagnosis of an infection with two opportunistic moulds. aspergillus terreus was also isolated from the secretions of the maxillary sinuses of the patient. in spite of antimycotic therapy, the child eventually died from respiratory failure.19947898514
superoxide anion production in response to bacterial lipopolysaccharide and fungal spores implicated in organic dust toxic syndrome.high amounts of fungal spores, bacteria, and bacterial endotoxin have been found in dust associated with the poorly characterized syndrome, organic dust toxic syndrome (odts). as part of an ongoing investigation to determine the etiopathogenesis for odts, this study has focused on activation of guinea pig bronchial alveolar lavage (bal) cells as evidenced by the production of superoxide anion in response to fungal spores and lipopolysaccharide (lps). fungal spores from aspergillus candidus, aspe ...19947925197
blasticidin s deaminase gene from aspergillus terreus (bsd): a new drug resistance gene for transfection of mammalian cells.blasticidin s deaminase (bsd) is a drug inactivating enzyme produced by aspergillus terreus, which convert blasticidin s (bs) to a non-toxic deamino-hydroxy derivative. the bsd gene was fused to sv 40 transcriptional regulatory elements and the resulting vector was used to transfect fm3a cells. expression of bsd conferred resistance to bs and allowed efficient isolation of integrative transfectants which have stably maintained the bs-resistance phenotype after repeated transfer to fresh selectiv ...19947948022
four new metabolites of aspergillus terreus.four new metabolites (1-4) were isolated from mycelium of aspergillus terreus ifo 6123 producing asterriquinone (arq). the structures of 1 and 2 were shown to be 3,6-bis[1-(1,1-dimethyl-2-propenyl)-1h-indol-3-yl]-furo[3,2-b]furan- 2,5-dione (asterridinone) and 2,5-bis[1-(1,1-dimethyl-2-propenyl)-1h-indol-3- yl]-3-acetoxy-6-hydroxy-2,5-cyclohexadiene-1,4-dione (arq monoacetate), respectively, by the chemical and spectral data. compounds 3 and 4 were identified with known asterriquinone isomers, 2 ...19947954921
effect of aspergillus terreus extract on neoplastic and non-neoplastic cell replication.there are very few and unconfirmed data regarding the antineoplastic activity of mycotic derivates in human cells. the effects on neoplastic and non-neoplastic cell replication of aspergillus terreus extracts have been tested. in fact, among different species of aspergillus mycotoxin producers. a terreus seems to be more suitable for hypothetical therapeutic purposes because of its low mycotoxin toxicity. very evident antiblastic activity of alcoholic crude extract of a. terreus on tumor cells h ...19948071682
polyketide synthase gene pksm from aspergillus terreus expressed during growth phase.the polyketide synthase gene pksm was detected in the genomic dna library of aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-msas) gene of penicillium patulum as a probe. 9524 bp of the cloned dna were sequenced and a 5.5 kb open reading frame was revealed. a single intron (62 bp) was identified in the conserved position. two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation ini ...19979438344
effects of territrem-b on cholinergic responses of snail neuron.effects of territrem-b (trb), a mycotoxin isolated from a rice culture of aspergillus terreus, on the central neuron of the snail achatina fulica were studied electrophysiologically. territrem-b potentiated the acetylcholine (ach) induced current of the neuron, while it had no effect on gaba or l-glutamate elicited currents. trb and neostigmine increased the peak amplitude of the response elicited by the first perfusion of ach and depressed the increase in current produced by a second perfusion. ...19938100056
articular aspergillosis: two case reports and review of the literature.we report two cases of septic arthritis of the knee caused by aspergillus: one by aspergillus terreus in a cirrhotic patient and the other by aspergillus fumigatus after vascular graft infection. the recovery of these organisms in synovial fluid should be considered as pathogenic, particularly in immunocompromised hosts. the pathogenic mechanism is discussed and the literature is reviewed.19979448597
detection of aspergillus ribosomal rna using biotinylated oligonucleotide probes.aspergillosis continues to be a devastating disease entity that results in significant mortality in immunosuppressed patients. rapid diagnosis is often required to initiate appropriate therapy. although the histopathologist may be able to visualize fungal organisms in tissue specimens, the histology of aspergillus species may overlap with a variety of fungi, so diagnosis often relies on fungal cultures that can take weeks to complete. recently, an in situ hybridization assay targeting aspergillu ...19979458383
[invasive bronchopulmonary aspergillosis treated with itraconazole in a patient with acute leukemia].a case of invasive broncopulmonar aspergillosis caused by aspergillus terreus successfully treated with itraconazole is reported in a patient undergoing autologous bone marrow transplantation for acute lymphoblastic leukaemia. although the patient was on prophylaxis with fluconazole and she did not respond to amphotericin b, there was an excellent response to itraconazole which allowed the transplant without any aspergillus infection during both the transplant and the post-transplant periods. du ...19938140504
riboflavin production by aspergillus terreus from beet-molasses.aspergillus terreus was used for riboflavin (vitamin b2) production in a medium containing beet molasses as the sole carbon source. growth and the vitamin production of the fungus were markedly affected by the composition of the culture medium. a maximum riboflavin yield was achieved at the late exponential growth phase (16 day-old cultures) in the presence of (g/l): centrifuged beet-molasses, 90; l-asparagine, 1; mgso4.7h2o, 0.5; k2hpo4/kh2po4 (1:1), 5; and the medium initially adjusted to ph 8 ...19938172689
a new nmr airlift bioreactor used in 31p-nmr studies of itaconic acid producing aspergillus airlift bioreactor for in-vivo nmr studies of cells is described. the 10-mm diameter airlift reactor was constructed for studies of mycelial/pellet forming organisms, grown in suspension. with this device 161 mhz 31p-nmr spectra of living aspergillus terreus cells, producing itaconic acid, have been obtained. signals were observed for intra- and extracellular orthophosphate, glycerol-3-phosphorylethanolamine (gpe), glycerol-3-phosphorylcholine (gpc), sugar phosphates and polyphosphate. the sp ...19938227940
comparison of in vitro activities of the new triazole sch56592 and the echinocandins mk-0991 (l-743,872) and ly303366 against opportunistic filamentous and dimorphic fungi and yeasts.the in vitro antifungal activities of sch56592, mk-0991, and ly303366 against 83 isolates of acremonium strictum, aspergillus flavus, aspergillus fumigatus, aspergillus terreus, bipolaris spp., blastomyces dermatitidis, cladophialophora bantiana, fusarium oxysporum, fusarium solani, histoplasma capsulatum, phialophora spp., pseudallescheria boydii, rhizopus arrhizus, scedosporium prolificans, and sporothrix schenckii were compared. the in vitro activities of these agents against 104 isolates of ...19989738049
effect of butyrolactone i on the producing fungus, aspergillus terreus.butyrolactone i [alpha-oxo-beta-(p-hydroxyphenyl)-gamma-(p-hydroxy-m-3, 3-dimethylallyl-benzyl)-gamma-methoxycarbonyl-gamma-butyrolactone] is produced as a secondary metabolite by aspergillus terreus. because small butyrolactone-containing molecules act as self-regulating factors in some bacteria, the effects of butyrolactone i on the producing organism were studied; specifically, changes in morphology, sporulation, and secondary metabolism were studied. threefold or greater increases in hyphal ...19989758788
endocarditis and aortal embolization caused by aspergillus terreus in a patient with acute lymphoblastic leukemia in remission: diagnosis by peripheral-blood culture.disseminated infection with aspergillus terreus is a rare disease that affects only the immunocompromised host. we report a case of systemic infection with a. terreus resulting in endocarditis, aortic embolization, and splenic infarction in a patient with acute lymphoblastic leukemia. diagnosis through peripheral blood culture, lack of pulmonary involvement, and onset of disease during complete remission from leukemia constitute uncommon features of this case.19989774591
aspergillus terreus postoperative endophthalmitis. 19938318491
purification and characterization of a regiospecific lipase from aspergillus terreus.aspergillus terreus lipase was purified to homogeneity with 18.0% yield. the specific activity of the enzyme increased from 20.80 to 250 u/mg of protein. ion exchange on q-sepharose was highly effective in the purification process. the molecular mass of the purified enzyme was 41+/-1 kda as determined by sds/page. the purified lipase showed excellent temperature tolerance (15-90 degreesc) and was highly thermostable, retaining 100% activity at 60 degreesc for 24 h. it showed good ph tolerance (3 ...19989799723
[the evaluation of antigens and antisera for their use in the serodiagnosis of aspergillosis].aspergillosis became an important opportunistic mycosis during the last years, with a great variety of clinical manifestations. to contribute to the replenishing of this mycosis serodiagnosis, biologic reactives (antigens and antisera) were prepared from strains of the species aspergillus niger, aspergillus flavus and aspergillus terreus for their use in immunoprecipitation assays. the reactives were assessed by double immunospreading versus a reference commercial system; satisfactory results we ...19959805081
aspergillus terreus during hospital renovation. 19938354865
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