galleria mellonella as a host model to study aspergillus terreus virulence and amphotericin b resistance.the aim of this study was to investigate if the alternative in vivo model galleria mellonella can be used (i) to determine differences in pathogenicity of amphotericin b (amb) resistant and susceptible a. terreus isolates, (ii) to evaluate amb efficacy in vivo (iii) and to correlate outcome to in vitro susceptibility data. larvae were infected with 2 a. terreus amb resistant (atr) and 3 amb susceptible (ats) isolates and survival rates were correlated to physiological attributes and killing abil ...201526107350
metabolic engineering of corynebacterium glutamicum for the production of itaconate.the capability of corynebacterium glutamicum for glucose-based synthesis of itaconate was explored, which can serve as building block for production of polymers, chemicals, and fuels. c. glutamicum was highly tolerant to itaconate and did not metabolize it. expression of the aspergillus terreus cad1 gene encoding cis-aconitate decarboxylase (cad) in strain atcc13032 led to the production of 1.4mm itaconate in the stationary growth phase. fusion of cad with the escherichia coli maltose-binding pr ...201526100077
amphotericin b resistance in aspergillus terreus is overpowered by coapplication of pro-oxidants.invasive fungal infections have significantly increased over the past decades in immunocompromised individuals and high-risk patients. amphotericin b (amb) exerts a powerful and broad activity against a vast array of fungi and has a remarkably low rate of microbial resistance. however, most isolates of aspergillus terreus developed an intrinsic resistance against amb, and during this study, we characterized the mode of action of this polyene antifungal drug in more detail in resistant (atr) and ...201526054424
combating drug resistant pathogenic bacteria isolated from clinical infections, with silver oxide nanoparticles.the antibiogram study of methicillin resistant staphylococcus aureus isolates revealed 100% resistance to vancomycin, bacitracin, erythromycin, ciprofloxacin and nalidixic acid. eight isolates (53.3%) showed resistance to co-trimoxazole and one isolate to rifampicin, which was the drug of choice. an effort was made to evaluate the antimethicillin resistant staphylococcus aureus activity of silver oxide (ag2o) nanoparticles synthesized from aspergillus terreus vit 2013. production of ag2o nanopar ...201726009646
experimental efficacy of anidulafungin against aspergillus terreus species complex.whereas echinocandins are alternatives for the treatment of invasive aspergillosis, the efficacy of anidulafungin (afg) against aspergillus terreus infection has not yet been explored. we have evaluated the in vitro activity, as well as the in vivo efficacy of afg in neutropenic mice infected by a. terreus species complex. time-kill studies showed in vitro fungistatic activity of afg against two strains. afg at doses of 5 and 10 mg/kg/day significantly reduced the fungal load in kidney of mice, ...201525980004
a new citrinin dimer isolated from aspergillus terreus strain zdf21.dicitrinin e (1), a new citrinin dimer, together with the known metabolites, dicitrinin a (2), citrinin (3), and fumitremorgin c (4), were isolated from the broth culture of aspergillus terreus strain zdf21. the structure of dicitrinin e (1) was elucidated through detailed analysis of 1d and 2d nmr experiments, cd and mass spectra the cytotoxicity of 1 was tested against larvae of artemia salina.201525973492
filamentous fungal endophthalmitis: results of combination therapy with intravitreal amphotericin b and report outcomes of exogenous fungal endophthalmitis treated with combination of intravitreal antifungal agents.201525926714
fungal artificial chromosomes for mining of the fungal secondary metabolome.with thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (sm) clusters 30-80 kb in size, breakthrough techniques are needed to characterize this sm wealth.201525925221
new meroterpenoids from aspergillus terreus with inhibition of cyclooxygenase-2 expression.two novel meroterpenoids, yaminterritrems a (1) and b (2), were isolated from aspergillus terreus collected from hot spring zones in yang-ming mountain, taiwan, and cultured at 40 °c. the structures of 1 and 2 were elucidated by nmr, ms spectral and x-ray crystallographic analyses. the biosynthetic route for 1 and 2 involving the conversion of the sesquiterpene with phenyl-α-pyrone is proposed. besides, 2 exhibited a dose-dependent inhibitory effect on cox-2 expression in lps-stimulated raw264.7 ...201525915447
blocking hsp70 enhances the efficiency of amphotericin b treatment against resistant aspergillus terreus strains.the polyene antifungal amphotericin b (amb) is widely used to treat life-threatening fungal infections. even though amb resistance is exceptionally rare in fungi, most aspergillus terreus isolates exhibit an intrinsic resistance against the drug in vivo and in vitro. heat shock proteins perform a fundamental protective role against a multitude of stress responses, thereby maintaining protein homeostasis in the organism. in this study, we elucidated the role of heat shock protein 70 (hsp70) famil ...201525870060
lovastatin production: from molecular basis to industrial process optimization.lovastatin, composed of secondary metabolites produced by filamentous fungi, is the most frequently used drug for hypercholesterolemia treatment due to the fact that lovastatin is a competitive inhibitor of hmg-coa reductase. moreover, recent studies have shown several important applications for lovastatin including antimicrobial agents and treatments for cancers and bone diseases. studies regarding the lovastatin biosynthetic pathway have also demonstrated that lovastatin is synthesized from tw ...201525868803
in vitro activities of amphotericin b, terbinafine, and azole drugs against clinical and environmental isolates of aspergillus terreus sensu stricto.the antifungal susceptibilities of 40 clinical and environmental isolates of a. terreus sensu stricto to amphotericin b, terbinafine, itraconazole, and voriconazole were determined in accordance with clsi document m38-a2. all isolates had itraconazole and voriconazole mics lower than epidemiologic cutoff values, and 5% of the isolates had amphotericin b mics higher than epidemiologic cutoff values. terbinafine showed the lowest mics. no significant differences were found when mics of clinical an ...201525824228
molecular epidemiology and in-vitro antifungal susceptibility of aspergillus terreus species complex isolates in delhi, india: evidence of genetic diversity by amplified fragment length polymorphism and microsatellite typing.aspergillus terreus is emerging as an etiologic agent of invasive aspergillosis in immunocompromised individuals in several medical centers in the world. infections due to a. terreus are of concern due to its resistance to amphotericin b, in vivo and in vitro, resulting in poor response to antifungal therapy and high mortality. herein we examined a large collection of molecularly characterized, geographically diverse a. terreus isolates (n = 140) from clinical and environmental sources in india ...201525781896
lumazine peptides from the marine-derived fungus aspergillus terreus.terrelumamides a (1) and b (2), two new lumazine-containing peptides, were isolated from the culture broth of the marine-derived fungus aspergillus terreus. from the results of combined spectroscopic and chemical analyses, the structures of these compounds were determined to be linear assemblies of 1-methyllumazine-6-carboxylic acid, an amino acid residue and anthranilic acid methyl ester connected by peptide bonds. these new compounds exhibited pharmacological activity by improving insulin sens ...201525775424
uncovering the unusual d-ring construction in terretonin biosynthesis by collaboration of a multifunctional cytochrome p450 and a unique isomerase.terretonin (1) is a fungal meroterpenoid isolated from aspergillus terreus, and possesses a highly oxygenated and unique tetracyclic structure. although the biosynthetic gene cluster for 1 has been identified and the biosynthesis has recently been studied by heterologous reconstitution and targeted-gene deletion experiments, the last few steps of the terretonin pathway after terrenoid (6) have yet to be elucidated. notably, the mechanism for the d-ring expansion to afford the terretonin scaffold ...201525671343
evaluation of antifungal combinations of nystatin-intralipid against aspergillus terreus using checkerboard and disk diffusion methods.the objective of this study was to evaluate the efficacy of combinations of nystatin-intralipid, found previously to be more active than nystatin, with antifungals of different mode of activity, against aspergillus terreus.201525639922
bioprocess engineering aspects of the cultivation of a lovastatin producer aspergillus terreus.the aim of this work is to review bioprocess engineering aspects of lovastatin (antihypercholesterolemia drug) production by aspergillus terreus in the submerged culture in the bioreactors of various scale presented in the scientific literature since the nineties of the twentieth century. the key factor influencing the cultivation of any filamentous species is fungal morphology and that is why this aspect was treated as the starting point for further considerations. fungal morphology is known to ...201525633258
influence of oxygen on lovastatin biosynthesis by aspergillus terreus atcc 20542 quantitatively studied on the level of individual pellets.despite oxygen is believed to be the most important environmental factor for any aerobic microbial process, the quantitative studies of its influence on growth and metabolite formation on the level of individual pellets formed by filamentous fungi were seldom performed. never was it made for lovastatin producer aspergillus terreus atcc20542. thus, this work is a quantitative study of oxygen transfer into a. terreus pellets during lovastatin biosynthesis in the shake flask culture. the basic meas ...201525627471
subcutaneous mycoses: an aetiological study of 15 cases in a tertiary care hospital at dibrugarh, assam, northeast india.subcutaneous mycoses are a group of fungal infections of dermis and subcutaneous tissue which consist of sporotrichosis, chromoblastomycosis, phaeohyphomycosis, hyalohyphomycosis, mycetoma, subcutaneous zygomycosis, rhinosporidiosis, lobomycosis and disseminated penicilliosis. a total of 46 consecutive patients with clinically suspected subcutaneous mycoses attending various departments of assam medical college and hospital were included in this prospective study to know the prevalence of subcut ...201525596859
(+)‑terrein inhibits human hepatoma bel‑7402 proliferation through cell cycle arrest.hepatoma is a common malignant tumor. thus, the development of a high‑efficacy therapeutic drug for hepatoma is required. in this study, (+)‑terrein isolated from the marine sponge‑derived aspergillus terreus pf‑26 against cell growth, apoptosis and cell cycle were assessed by mtt and flow cytometry. mrna array containing 73 cell cycle‑related genes and three cell morphology‑related genes was generated and its performance evaluated. the cell cycle pathway map was created using the pathview packa ...201525592371
onychomycosis in israel: epidemiological aspects.onychomycosis is a fungal infection treated orally for prolonged periods of treatment, caused primarily by dermatophytes, candida species and non-dermatophyte moulds (ndms). the prevalence of specific aetiology may differ in dependence of environmental, geographic and demographic factors, and may affect management of the infection. the objective of this survey was to analyse epidemiologic parameters of onychomycosis in israel. data of a cohort of 27,093 patients were collected from six centres d ...201525590832
anticancer activity of fungal l-asparaginase conjugated with zinc oxide nanoparticles.demand for developing novel delivery system for cancer treatment has increased due to the side effects present in intravenous injection of l-asparaginase. nanoparticles are used for delivering the drugs to its destination in cancer cure. nanobiocomposite of zinc oxide nanoparticles conjugated with l-asparaginase was produced by aspergillus terreus and was confirmed using maximum uv-vis absorption at 340 nm in the present work. the presence of functional groups like oh, c-h, -c=n and c=o on the s ...201525589205
recent advances in genome mining of secondary metabolites in aspergillus terreus.filamentous fungi are rich resources of secondary metabolites (sms) with a variety of interesting biological activities. recent advances in genome sequencing and techniques in genetic manipulation have enabled researchers to study the biosynthetic genes of these sms. aspergillus terreus is the well-known producer of lovastatin, a cholesterol-lowering drug. this fungus also produces other sms, including acetylaranotin, butyrolactones, and territram, with interesting bioactivities. this review wil ...201425566227
[superficial mycoses in patients from anzoátegui state, venezuela, period 2002-2012].superficial fungal diseases that affect the skin and its appendages are frequently seen in basic triage and in dermatology services. these diseases are distributed in venezuela with an incidence of 92.9%. the aim of this study was to determine the genera and species that cause dermatomycoses in residents of anzoátegui state, venezuela, during the period 2002-2012. a total of 4257 patients with a presumptive diagnosis of superficial mycoses were studied, with ages from 7 months to 79 years. the o ...201425558751
antifungal activity of compounds targeting the hsp90-calcineurin pathway against various mould species.invasive mould infections are associated with a high mortality rate and the emergence of mdr moulds is of particular concern. calcineurin and its chaperone, the heat shock protein 90 (hsp90), represent an important pathway for fungal virulence that can be targeted at different levels. we investigated the antifungal activity of compounds directly or indirectly targeting the hsp90-calcineurin axis against different mould species.201525558076
simultaneous saccharification and fermentation of delignified lignocellulosic biomass at high solid loadings by a newly isolated thermotolerant kluyveromyces sp. for ethanol production.simultaneous saccharification and fermentation studies were carried out using thermotolerant newly isolated kluyveromyces sp. with three different delignified lignocellulosic biomass viz. rice straw, wheat straw and sugarcane bagasse at 5-15% solid loading and 6-12 fpu g(-1) substrate enzyme loading for different time intervals 0-72 h at 42°c. maximum ethanol achieved from rice straw, wheat straw and sugarcane bagasse with in-house crude cellulases from aspergillus terreus was 23.23, 18.29 and 1 ...201525553563
purification and characterization of detergent-compatible protease from aspergillus terreus gr.the possibility of using aspergillus terreus protease in detergent formulations was investigated. sodium dodecyl sulfate (sds) and native polyacrylamide gel electrophoresis indicated that the purified alkaline protease (148.9 u/mg) is a monomeric enzyme with a molecular mass of 16 ± 1 kda. this was confirmed by liquid chromatography-mass spectrometry. the active enzyme degraded the co-polymerized gelatin. the protease demonstrated excellent stability at ph range 8.0-12.0 with optimum at ph 11.0. ...201528324360
effect of reduced oxygen on the antifungal susceptibility of clinically relevant aspergilli.the influence of hypoxia on the in vitro activities of amphotericin b, azoles, and echinocandins against aspergillus spp. was evaluated by comparing mics, minimal fungicidal concentrations (mfcs), and epidemiological cutoffs (ecoffs). changes of mic distributions due to hypoxia largely depend on the method, the species, and the growth ability under hypoxia. the activities of antifungals were not significantly altered under hypoxia, except for aspergillus terreus, for which the activity changed f ...201525547350
territrem and butyrolactone derivatives from a marine-derived fungus aspergillus terreus.seventeen lactones including eight territrem derivatives (1-8) and nine butyrolactone derivatives (9-17) were isolated from a marine-derived fungus aspergillus terreus scsgaf0162 under solid-state fermentation of rice. compounds 1-3 and 9-10 were new, and their structures were elucidated by spectroscopic analysis. the acetylcholinesterase inhibitory activity and antiviral activity of compounds 1-17 were evaluated. among them, compounds 1 and 2 showed strong inhibitory activity against acetylchol ...201425522319
aspergillus citrinoterreus, a new species of section terrei isolated from samples of patients with nonhematological predisposing conditions.the use of molecular identification techniques has revealed an increasing number of new species within aspergillus section terrei. we phenotyped a set of 26 clinical isolates that showed genetic differences from aspergillus terreus sensu stricto by analyzing sequences from pcr-amplified β-tubulin and calmodulin genes and the internal transcribed spacer region. since the isolates were phylogenetically and morphologically different from all of the members of aspergillus section terrei, they are de ...201525502530
immobilization of aspergillus terreus lipase in self-assembled hollow nanospheres for enantioselective hydrolysis of ketoprofen vinyl ester.the aim of this study was to improve the ability of aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/α-cyclodextrins (alg-g-peg/α-cd) spheres as enzyme immobilization carriers. the morphology and size of the alg-g-peg/α-cd particles were investigated by transmission electron microscopy (tem) and were found to be nanoscale. to facilitate recycling, calcium alginate (ca) beads were ...201525483320
production of itaconic acid in escherichia coli expressing recombinant α-amylase using starch as substrate.several studies on fermentative production of a vinyl monomer itaconic acid from hydrolyzed starch using aspergillus terreus have been reported. herein, we report itaconic acid production by escherichia coli expressing recombinant α-amylase, using soluble starch as its sole carbon source. to express α-amylase in e. coli, we first constructed recombinant plasmids expressing α-amylases by using cell surface display technology derived from two amylolytic bacteria, bacillus amyloliquefaciens nbrc 15 ...201525468427
reactive oxygen species regulate lovastatin biosynthesis in aspergillus terreus during submerged and solid-state a previous work we detected an important increase in reactive oxygen species (ros) concentrations during idiophase in lovastatin fermentations. hence, the objective of the present work was to determine if ros contributes to the regulation of lovastatin biosynthesis. exogenous antioxidants were used to reduce ros accumulation. the addition of n-acetyl-l-cysteine (nac) decreased ros accumulation and concurrent lovastatin production. in solid-state fermentation (ssf), the addition of 100 mm of n ...201425457945
expression, characterization and mutagenesis of an fad-dependent glucose dehydrogenase from aspergillus fad-dependent glucose dehydrogenase (fad-gdh) from aspergillus terreus nih2624 was expressed in escherichia coli with a yield of 228±16u/l of culture. co-expression with chaperones dnak/dnaj/grpe and osmotic stress induced by simple carbon sources enhanced productivity significantly, improving the yield to 23883±563u/l after optimization. fad-gdh was purified in two steps with the specific activity of 604u/mg. using d-glucose as substrate, the optimal ph and temperature for fad-gdh were deter ...201525435504
the overproduction of 2,4-dtbp accompanying to the lack of available form of phosphorus during the biodegradative utilization of aminophosphonates by aspergillus terreus.although information about the ability of some filamentous fungi to biodegrade organophosphonates is available, the knowledge about accompanying changes in fungal metabolism is very limited. the aim of our study was to determine the utilization of the chosen, structurally diverse aminophosphonates by aspergillus terreus (thom), in the context of the behaviour of this fungus while growing in unfavourable conditions, namely the lack of easily available phosphates. we found that all the studied com ...201525385070
aspergillus terreus infection in a sutureless self-sealing incision made during cataract, we describe a case of keratitis caused by aspergillus terreus in an 80-year-old immunocompetent woman 1 month after uneventful cataract surgery. the patient presented with decreased visual acuity (20/50) and severe pain in her right eye. examination revealed a 3.5 × 4.5 mm white-colored deep stromal infiltration located on the temporal corneal tunnel incision. corneal scraping samples were obtained for cytological and culture examinations. the cinnamon-brown colonies consisting of a dense ...201525362536
fungal production of single cell oil using untreated copra cake and evaluation of its fuel properties for biodiesel.this study evaluated the microbial conversion of coconut oil waste, a major agro-residue in tropical countries, into single cell oil (sco) feedstock for biodiesel production. copra cake was used as a low-cost renewable substrate without any prior chemical or enzymatic pretreatment for submerged growth of an oleaginous tropical mangrove fungus, aspergillus terreus ibb m1. the sco extracted from fermented biomass was converted into fatty acid methyl esters (fames) by transesterification and evalua ...201525341469
the marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells.the difficulties faced in the effective treatment of ovarian cancer are multifactorial, but are mainly associated with relapse and drug resistance. cancer stem-like cells have been reported to be an important contributor to these hindering factors. in this study, we aimed to investigate the anticancer activities of a bioactive fungal metabolite, namely terrein, against the human epithelial ovarian cancer cell line, skov3, primary human ovarian cancer cells and ovarian cancer stem-like cells. ter ...201425318762
metabolic engineering of itaconate production in escherichia coli.interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. itaconic acid, a c5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (cada) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into itaconate. heterologous expression of cada from aspergillus terreus in escherichia coli resulted in low cada acti ...201525277412
molecular genetic characterization of terreic acid pathway in aspergillus terreus.terreic acid is a natural product derived from 6-methylsalicylic acid (6-msa). a compact gene cluster for its biosynthesis was characterized. isolation of the intermediates and shunt products from the mutant strains, combined with bioinformatic analyses, allowed for the proposition of a biosynthetic pathway for terreic acid.201425265334
aspergillus terreus endogenous endophthalmitis: report of a case and review of literature.we report a rare case of aspergillus terreus endogenous endophthalmitis in an immunocompetent patient with subretinal abscess and also review the reported cases. a 50-year-old healthy male presented with sudden painful loss of vision in right eye. he was diagnosed with endogenous endophthalmitis and underwent urgent vitrectomy. aspergillus terreus growth was obtained in culture. at final follow-up, there was complete resolution of the infection but visual acuity was poor due to macular scar. asp ...201425230968
influence of the ph on the itaconic acid production with aspergillus terreus.itaconic acid is mainly produced with the filamentous fungi aspergillus terreus. an increase in the ph during the production phase of the cultivation resulted in an increase in the itaconic acid concentration. the ph was raised by a single ph shift ranging from ph 4 to 6 or by a ph control to ph 3. different lyes can be used for the ph shift, but ammonia solution has proven to be the best, because here the productivity does not drop after the ph shift. the highest itaconic acid concentration of ...201425213913
treatment of aspergillus terreus infections: a clinical problem not yet resolved.despite the use of recommended therapies, invasive infections by aspergillus terreus show a poor response. for years, investigative studies on the failure of therapy of fungal infections have focused on in vitro susceptibility data. however, it is well known that low minimum inhibitory concentrations (mics) are not always predictive of response to therapy despite a correct dosage schedule. many experimental and clinical studies have tried to establish a relationship between mics and outcome in s ...201425190543
improving itaconic acid production through genetic engineering of an industrial aspergillus terreus strain.itaconic acid, which has been declared to be one of the most promising and flexible building blocks, is currently used as monomer or co-monomer in the polymer industry, and produced commercially by aspergillus terreus. however, the production level of itaconic acid hasn't been improved in the past 40 years, and mutagenesis is still the main strategy to improve itaconate productivity. the genetic engineering approach hasn't been applied in industrial a. terreus strains to increase itaconic acid p ...201425162789
direct production of itaconic acid from liquefied corn starch by genetically engineered aspergillus terreus.itaconic acid is on the doe (department of energy) top 12 list of biotechnologically produced building block chemicals and is produced commercially by aspergillus terreus. however, the production cost of itaconic acid is too high to be economically competitive with the petrochemical-based products. itaconic acid is generally produced from raw corn starch, including three steps: enzymatic hydrolysis of corn starch into a glucose-rich syrup by α-amylase and glucoamylase, fermentation, and recovery ...201425162619
successful surgical treatment of rare aspergillus terreus prosthetic valve endocarditis complicated by intracranial and mesenteric artery mycotic aneurysms. 201425156465
synthetic (+)-terrein suppresses interleukin-6/soluble interleukin-6 receptor induced-secretion of vascular endothelial growth factor in human gingival fibroblasts.interleukin (il)-6 is a proinflammatory cytokine that performs a wide variety of biological functions, including important roles in the progression of chronic inflammatory diseases such as periodontal disease. (+)-terrein, a secondary bioactive fungal metabolite isolated from aspergillus terreus, has various biological activities; however, its anti-inflammatory effects are still unknown. the purpose of this study was to examine the effect of synthetic (+)-terrein on il-6 signaling and related pr ...201425151086
cellulolytic and xylanolytic enzymes from thermophilic aspergillus terreus rwy.thermophilic aspergillus terreus rwy produced cellulases and xylanases in optimal concentrations at 45 °c in solid state fermentation process, though enzyme production was also observed at 50 and 55 °c. filter paper cellulase (fp), endoglucanase (eg), β-glucosidase (bgl), cellobiohydrolase (cbh), xylanase, β-xylosidase, α-l-arabinofuranosidase and xylan esterase activities for a. terreus rwy at 45 °c in 72 h were 11.3 ± 0.65, 103 ± 6.4, 122.5 ± 8.7, 10.3 ± 0.66, 872 ± 22.5, 22.1 ± 0.75, 126.4 ±  ...201425047723
optimization of β-glucosidase production by aspergillus terreus strain emoo 6-4 using response surface methodology under solid-state fermentation.forty-two morphologically different fungal strains were isolated from different soil samples and agricultural wastes and screened for β-glucosidase activity under solid-state fermentation. eight species were chosen as the most active β-glucosidase producers and were subjected to primary morphological identification. β-glucosidase was highly produced by aspergillus terreus, which showed the highest activity, and was subjected to full identification using scanning electron microscopy and molecular ...201525036937
efficient expression, purification, and characterization of a novel fad-dependent glucose dehydrogenase from aspergillus terreus in pichia pastoris.flavin adenine dinucleotide-dependent glucose dehydrogenase (fad-gdh) can utilize a variety of external electron acceptors and also has stricter substrate specificity than any other glucose oxidoreductases, which makes it the ideal diagnostic enzyme in the field of glucose biosensors. a gene coding for a hypothetical protein, similar to glucose oxidase and derived from aspergillus terreus nih2624, was overexpressed in pichia pastoris gs115 under the control of an aox1 promoter with a level of 26 ...201425022525
invasive aspergillosis caused by aspergillus terreus: an emerging opportunistic infection with poor outcome independent of azole therapy.invasive aspergillosis (ia) caused by aspergillus terreus is a significant cause of morbidity and mortality in patients with haematological malignancy (hm). very few data are available in this patient population to differentiate ia patients with a. terreus from those with non-terreus species of aspergillus to compare outcomes. we retrospectively investigated 513 hm patients who were treated for either definite or probable ia between june 1993 and august 2012 in a cancer centre.201425006241
aspergillus terreus recovered from a corneal scraping.a 52 year old, healthy male presented to his optometrist complaining of redness and irritation in the right eye. a foreign body was removed from the eye. the patient was started on ophthalmic solutions of vigamox and systane. at 48 hours, the patient reported increased redness, limited vision, and yellow discharge from the eye. the patient was referred to an ophthalmologist for further evaluation. physical assessment revealed a superlative central infiltrate (extreme, centrally located injury th ...201425000648
promiscuity breeds diversity: the role of polyketide synthase in natural product biosynthesis.secondary metabolites are often highly biologically active molecules and are widely used from antibacterial to anticancer drugs. in this issue of chemistry and biology, zaehle and coworkers describe the gene cluster and biosynthesis of the polyketide terrein, a secondary metabolite produced by the soil-borne fungus aspergillus terreus.201424950100
enhancement of in vitro high-density polyethylene (hdpe) degradation by physical, chemical, and biological treatments.partially degraded high-density polyethylene (hdpe) was collected from plastic waste dump yard for biodegradation using fungi. of various fungi screened, strain mf12 was found efficient in degrading hdpe by weight loss and fourier transform infrared (ft-ir) spectrophotometric analysis. strain mf12 was selected as efficient hdpe degraders for further studies, and their growth medium composition was optimized. among those different media used, basal minimal medium (bmm) was suitable for the hdpe d ...201424946709
invasion of solanum tuberosum l. by aspergillus terreus: a microscopic and proteomics insight on pathogenicity.aspergillus terreus is one of the most harmful filamentous fungal pathogen of humans, animals and plants. recently, researchers have discovered that a. terreus can cause foliar blight disease in potato (solanum tuberosum l.). we used light and scanning electron microscopy, and performed proteomics analysis in an attempt to dissect the invasion process of a. terreus in this important crop.201424917207
molecular characterization and expression of a novel alcohol oxidase from aspergillus terreus mtcc6324.the alcohol oxidase (aox) cdna from aspergillus terreus mtcc6324 with an open reading frame (orf) of 2001 bp was constructed from n-hexadecane induced cells and expressed in escherichia coli with a yield of ∼4.2 mg protein g-1 wet cell. the deduced amino acid sequences of recombinant raox showed maximum structural homology with the chain b of aryl aox from pleurotus eryngii. a functionally active aox was achieved by incubating the apo-aox with flavin adenine dinucleotide (fad) for ∼80 h at 16°c ...201424752075
filamentous fungi in microtiter plates-an easy way to optimize itaconic acid production with aspergillus terreus.itaconic acid is an important industrial building block and is produced by the filamentous fungi aspergillus terreus. to make the optimization process more efficient, a scale-down from shake flasks to microtiter plates was performed. this resulted in comparable product formations, and 87.7 g/l itaconic acid was formed after 10 days of cultivation in the microtiter plate. the components of the minimal medium were varied independently for a media optimization. this resulted in an increase of the i ...201424737061
upstream and downstream processing of lovastatin by aspergillus terreus.the present study describes the enhanced production and purification of lovastatin by aspergillus terreus in submerged batch fermentation. the enhancement of lovastatin production from a. terreus was attempted by random mutagenesis using ultraviolet radiations and nitrous acid. uv mutants exhibited increased efficiency for lovastatin production as compared with nitrous acid mutants. among all the mutants developed, a. terreus uv-4 was found to be the hyper producer of lovastatin. this mutant gav ...201424671671
expression, purification, crystallization and preliminary x-ray diffraction analysis of aspergillus terreus endo-β-1,4-glucanase from glycoside hydrolase family 12.endoglucanases are important enzymes that are involved in the modification and degradation of cellulose. filamentous fungi such as aspergillus terreus are effective biomass degraders in nature owing to their capacity to produce an enzymatic arsenal of glycoside hydrolases, including endoglucanase from glycoside hydrolase family 12 (gh12). the a. terreus gh12 endoglucanase was cloned and overexpressed in a. nidulans, purified and crystallized. a single crystal was obtained from a solution consist ...201424637772
culture-based and sequence-based insights into biosynthesis of secondary metabolites by aspergillus terreus atcc 20542.aspergillus terreus atcc 20542 was cultivated in various culture media in order to activate its genome-encoded biosynthetic pathways and explore the secondary metabolic repertoire. in addition to mevinolinic acid (lovastatin) and its precursor monacolin l, a number of other secondary metabolites were found in the analyzed cultures, namely terreic acid, citrinin, (+)-geodin, terrein, and dehydrocurvularin. in contrast to previously described gene clusters responsible for production of lovastatin, ...201424534845
dissimilatory nitrate reduction by aspergillus terreus isolated from the seasonal oxygen minimum zone in the arabian sea.a wealth of microbial eukaryotes is adapted to life in oxygen-deficient marine environments. evidence is accumulating that some of these eukaryotes survive anoxia by employing dissimilatory nitrate reduction, a strategy that otherwise is widespread in prokaryotes. here, we report on the anaerobic nitrate metabolism of the fungus aspergillus terreus (isolate an-4) that was obtained from sediment in the seasonal oxygen minimum zone in the arabian sea, a globally important site of oceanic nitrogen ...201424517718
optimization of inulinase production from low cost substrates using plackett-burman and taguchi methods.four marine-derived fungal isolates were screened for the production of inulinase enzyme from low cost substrates under solid state fermentation (ssf), one of them identified as aspergillus terreus showed the highest inulinase activity using artichoke leaves as a solid substrate. sequential optimization strategy, based on statistical experimental designs was employed to optimize the composition of the medium, including plackett-burman and taguchi's (l9 3(4)) orthogonal array designs. under the o ...201424507281
optimization of complex fermentation media for glucose oxidase production using statistical approach.production cost of enzyme is largely determined by the type of the strain and raw material used to propagate the strain. hence, selection of the strain and raw materials is crucial in enzyme production. for glucose oxidase (gox), previous studies showed aspergillus terreus unimap aa-1 offers a better alternative to the existing sources. thus, a lower production cost could be logically anticipated by growing the strain in a cheaper complex media such as molasses. in this work, sugar cane molasses ...201324502155
studies on extraction of mannanase enzyme by aspergillus terreus suk-1 from fermented palm kernel cake.microbial mannanases have become biotechnologically important in industry but their application is limited due to high production cost. in presents study, the extraction of mannanase from fermented palm kernel cake (pkc) in the solid state fermentation (ssf) was optimized. local isolate of aspergillus terreus suk-1 was grown on pkc in (ssf) using column bioreactor. the optimum condition were achieved after two washes of fermented pkc by adding of 10% glycerol (v/v) soaked for 10 h at the room te ...201324502150
potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses.ionic liquids (ils) have been used as an alternative green solvent for lignocelluloses pretreatment. however, being a salt, ils exhibit an inhibitory effect on cellulases activity, thus making the subsequent saccharification inefficient. the aim of the present study is to produce salt-tolerant cellulases, with the rationale that the enzyme also tolerant to the presence of ils. the enzyme was produced from a locally isolated halophilic strain and was characterized and assessed for its tolerance t ...201424457303
aryl-aldehyde formation in fungal polyketides: discovery and characterization of a distinct biosynthetic mechanism.aryl-aldehydes are a common feature in fungal polyketides, which are considered to be exclusively generated by the r domain of nonreducing polyketide synthases (nr-pkss). however, by cloning and heterologous expression of both cryptic nr-pks and nonribosomal peptide synthase (nrps)-like genes from aspergillus terreus in saccharomyces cerevisiae, we identified a distinct mechanism for aryl-aldehyde formation in which a nrps-like protein activates and reduces an aryl-acid produced by the accompany ...201424412543
butyrolactone i quantification from lovastatin producing aspergillus terreus using tandem mass spectrometry-evidence of signalling functions.aspergillus terreus is an industrially important filamentous fungus producing a wide spectrum of secondary metabolites, including lovastatin and itaconic acid. it also produces butyrolactone i which has shown potential as an antitumour agent. additionally, butyrolactone i has been implicated to have a regulating role in the secondary metabolism and morphology of a. terreus. in this study, a quantitative time-course liquid chromatography-electrospray ionisation-tandem mass spectrometry (lc-esi-ms ...201427682234
new rubrolides from the marine-derived fungus aspergillus terreus oucmdz-1925.two new rubrolides, rubrolides r (1) and s (2), were isolated from the fermentation broth of the marine-derived fungus aspergillus terreus oucmdz-1925. their structures were elucidated on the basis of spectroscopic analysis and x-ray single crystal diffraction. compound 1 showed comparable or superior antioxidation against 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (abts) radicals to those of trolox and ascorbic acid with an ic₅₀ value of 1.33 mm. compound 2 showed comparable or superi ...201424326339
functional analysis of cis-aconitate decarboxylase and trans-aconitate metabolism in riboflavin-producing filamentous ashbya ashbya gossypii, isocitrate lyase (icl1) is a very crucial enzyme for riboflavin production. itaconate, the inhibitor of icl1, has been used as an antimetabolite for mutagenic studies in a. gossypii. it has been reported that itaconate is produced from cis-aconitate by cis-aconitate decarboxylase (cad1) in aspergillus terreus. in this study, identification of cad1 gene and determination of the presence of itaconate in the riboflavin biosynthetic pathway in a. gossypii were carried out to conf ...201424315530
does a rhizospheric microorganism enhance k⁺ availability in agricultural soils?the potassium solubilizing microorganisms (ksms) are a rhizospheric microorganism which solubilizes the insoluble potassium (k) to soluble forms of k for plant growth and yield. k-solubilization is carried out by a large number of saprophytic bacteria (bacillus mucilaginosus, bacillus edaphicus, bacillus circulans, acidothiobacillus ferrooxidans, paenibacillus spp.) and fungal strains (aspergillus spp. and aspergillus terreus). major amounts of k containing minerals (muscovite, orthoclase, bioti ...201424315210
n-glycosylation affects the proper folding, enzymatic characteristics and production of a fungal ß-glucosidase.heterologous expression of ß-glucosidase is one of the approaches to enhance the efficiency of fungal cellulase preparations. it has been reported that n-glycosylation affects the structure framework, function and stability of proteins. in this study, a ß-glucosidase from aspergillus terreus (genbank: xp_001216552, bgls) was heterologously expressed in pichia pastoris and trichoderma reesei. the four asparagine residues were all linked with high-mannose-type oligosaccharides in p. pastoris, wher ...201324308062
characterization of the co-purified invertase and β-glucosidase of a multifunctional extract from aspergillus terreus.the filamentous fungus aspergillus terreus secretes both invertase and β-glucosidase when grown under submerged fermentation containing rye flour as the carbon source. the aim of this study was to characterize the co-purified fraction, especially the invertase activity. an invertase and a β-glucosidase were co-purified by two chromatographic steps, and the isolated enzymatic fraction was 139-fold enriched in invertase activity. sds-page analysis of the co-purified enzymes suggests that the prote ...201424307498
cloning, characterization and application of a glyceraldehyde-3-phosphate dehydrogenase promoter from aspergillus is important to develop native and highly efficient promoters for effective genetic engineering of filamentous fungi. although aspergillus terreus is an important industrial fungus for the production of itaconic acid and lovastatin, the available genetic toolbox for this microorganism is still rather limited. we have cloned the 5' upstream region of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd; 2,150 bp from the start codon) from a. terreus cicc 40205 and subsequently confirmed its ...201424306453
a systems biology approach for the identification of target genes for the improvement of itaconic acid production in aspergillus this paper, a clone based transcriptome analysis towards the identification of genes related to itaconic acid production in aspergillus terreus was carried out as an extension of a previously published a clone-based transcriptome analysis from a set of batch fermentation experiments. also a publically available a. niger transcriptome dataset from cultures similar to those of the a. terreus data set was analyzed to evaluate the specificity of the approach followed for a. terreus.201324304666
relationship between morphology and itaconic acid production by aspergillus terreus.the morphology of filamentous fungi closely correlates with the productivity in submerged culture. using itaconic acid (ia) production by aspergillus terreus as a research model, the quantitative relationship between the growth form of a. terreus and ia production was investigated. ia fermentation was scaled up from shake flasks to a 7 l stirred tank bioreactor based on the quantitative relationship. our results demonstrated the following: (1) three morphologies of a. terreus were formed by chan ...201424169454
unusual biotransformation products of the sesquiterpene lactone budlein a by aspergillus species.biotransformation of chemicals by microorganisms can be effective in increasing chemical diversity. some fungi have been described to be useful for the biotransformation of sesquiterpene lactones. nevertheless, in most cases, only minor or simple transformations of functional groups have been observed. budlein a is a sesquiterpene lactone found in high amounts in american sunflower-like species of the genus viguiera (asteraceae). it shows important biological effects like in vitro and in vivo an ...201324135634
biodegradation of a mixture of pahs by non-ligninolytic fungal strains isolated from crude oil-contaminated soil.nine native non-ligninolytic fungal strains were isolated from maya crude oil-contaminated soil and selected based on their ability to grow and use crude oil and several polycyclic aromatic hydrocarbons (pahs) as carbon source, for their application to pah removal in soil. the fungi were identified by pcr amplification of intergenic transcribed sequences regions and microbiological techniques, and results showed them to be part of the genera fusarium, neurospora, aspergillus, scedosporium, penic ...201424132496
rapid determination of lovastatin in the fermentation broth of aspergillus terreus using dual-wavelength uv spectrophotometry.lovastatin, a hypocholesterolemic drug, is produced by submerged fermentation of aspergillus terreus thom (trichocomaceae). high performance liquid chromatography is usually used to determine lovastatin in samples of the fermentation broth. however, this method is inconvenient and costly, especially in the context of high-throughput sample analysis.201424093797
discovery of a linoleate 9s-dioxygenase and an allene oxide synthase in a fusion protein of fusarium oxysporum.fusarium oxysporum is a devastating plant pathogen that oxidizes c₁₈ fatty acids sequentially to jasmonates. the genome codes for putative dioxygenase (dox)-cytochrome p450 (cyp) fusion proteins homologous to linoleate diol synthases (ldss) and the allene oxide synthase (aos) of aspergillus terreus, e.g., foxb_01332. recombinant foxb_01332 oxidized 18:2n-6 to 9s-hydroperoxy-10(e),12(z)-octadecadienoic acid by hydrogen abstraction and antarafacial insertion of molecular oxygen and sequentially to ...201324082064
chemoenzymatic synthesis of α-l-rhamnosides using recombinant α-l-rhamnosidase from aspergillus terreus.this study describes an efficient, large scale fermentation of a recombinant α-l-rhamnosidase originating from aspergillus terreus. high-cell-density pichia pastoris fermentation resulted in yields up to 627 u/l/h. the recombinant enzyme was used for the reverse rhamnosylation of various small organic compounds. a full factorial experimental design setup was applied to identify the importance of temperature, substrate concentrations, solvent type and concentration as well as the acidity of the r ...201324012095
in vitro antimicrobial potential of the lichen parmotrema sp. extracts against various pathogens.the ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. the perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. this study was aimed to explore in vitro antim ...201323997920
application of scale-up criterion of constant oxygen mass transfer coefficient (kla) for production of itaconic acid in a 50 l pilot-scale fermentor by fungal cells of aspergillus terreus.the scale-up criterion of constant oxygen mass transfer coefficient (kla) was applied for the production of itaconic acid (ia) in a 50 l pilot-scale fermentor by the fungal cells of aspergillus terreus. various operating conditions were examined to collect as many kla data as possible by adjusting the stirring speed and aeration rate in both 5 l and 50 l fermentor systems. in the fermentations performed with the 5 l fermentor, the highest ia production was obtained under the operating conditions ...201323928842
spiro fused diterpene-indole alkaloids from a creek-bottom-derived aspergillus terreus.four metabolites, teraspiridoles a-d (2-5), formed from the merger of a diterpene and modified indole scaffold were obtained from an aspergillus terreus isolate. the structures and absolute configurations of these natural products were established using nmr, mass spectrometry, marfey's method, vcd, and ecd data. teraspiridole b (3) exhibited weak inhibition of planaria regeneration/survival.201323924243
mechanistic insights from the binding of substrate and carbocation intermediate analogues to aristolochene synthase.aristolochene synthase, a metal-dependent sesquiterpene cyclase from aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (fpp) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. here, we report the x-ray crystal structure of aristolochene synthase complexed with three mg(2+) ions and the unreactive substrate analogue farnesyl-s-thiolodiphosphate (fspp), showing that the substrate diphosphate group is a ...201323905850
two β-xylanases from aspergillus terreus: characterization and influence of phenolic compounds on xylanase activity.sugarcane bagasse was used as an inexpensive alternative carbon source for production of β-xylanases from aspergillus terreus. the induction profile showed that the xylanase activity was detected from the 6th day of cultivation period. two low molecular weight enzymes, named xyl t1 and xyl t2 were purified to apparent homogeneity by ultrafiltration, gel filtration and ion exchange chromatographies and presented molecular masses of 24.3and 23.60 kda, as determined by sds-page, respectively. xyl t ...201323892064
secondary metabolites of a mangrove endophytic fungus aspergillus terreus (no. gx7-3b) from the south china sea.the mangrove endophytic fungus aspergillus terreus (no. gx7-3b) was cultivated in potato dextrose liquid medium, and one rare thiophene compound (1), together with anhydrojavanicin (2), 8-o-methylbostrycoidin (3), 8-o-methyljavanicin (4), botryosphaerone d (5), 6-ethyl-5-hydroxy-3,7-dimethoxynaphthoquinone (6), 3β,5α-dihydroxy-(22e,24r)-ergosta-7,22-dien-6-one (7), 3β,5α,14α-trihydroxy-(22e,24r)-ergosta-7, 22-dien-6-one (8), nga0187 (9) and beauvericin (10), were isolated. their structures were ...201323877026
purification and characterization of a hydrolysis-resistant lipase from aspergillus terreus.lipase from aspergillus terreus was purified to homogeneity using ammonium sulfate precipitation and chromatographies with q-sepharose and sephacryl s-200. it showed a single band on sds-page and ief-page with a relative molecular mass of 37.2 kda and pi of 3.2. its glycoprotein nature was confirmed with the percentage of saccharides of 5.02% and 3.88% determined by the phenol/sulfuric acid and anthrone/ sulfuric acid methods, respectively. the lipase hydrolyzed both plant oils and animal oils, ...201423855368
application of an efficient gene targeting system linking secondary metabolites to their biosynthetic genes in aspergillus terreus.nonribosomal peptides (nrps) are natural products biosynthesized by nrp synthetases. a kusa-, pyrg- mutant strain of aspergillus terreus nih 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. application of this tool allowed us to link four major types of nrp-related secondary metabolites to their responsible genes in a. terreus. in addition, an nrp affecting melanin synthesis was also identified in this species.201323841722
asperterrestide a, a cytotoxic cyclic tetrapeptide from the marine-derived fungus aspergillus terreus scsgaf0162.a new cytotoxic and antiviral cyclic tetrapeptide, asperterrestide a (1), a new alkaloid, terremide c (2), and a new aromatic butenolide, aspernolide e (3), together with 10 known compounds were isolated from the fermentation broth of the marine-derived fungus aspergillus terreus scsgaf0162. their structures were elucidated by spectroscopic analysis, and the absolute configuration of 1 was determined by the mosher ester technique and analysis of the acid hydrolysates using a chiral-phase hplc co ...201323806112
rep-pcr and rapd-pcr fingerprinting of aspergillus terreus.we compared two pcr methods for molecular typing the medically important filamentous fungus aspergillus terreus. in a set of 46 strains investigated we found 19 and 12 different fingerprinting types obtained by random amplified polymorphic dna pcr (rapd) and semi-automated repetitive element pcr (rep-pcr), respectively.201323758105
virulence and thrombocyte affectation of two aspergillus terreus isolates differing in amphotericin b susceptibility.aspergillus terreus-induced invasive infections exhibit high lethality, partly due to the intrinsic resistance for amphotericin b (amb). we compared the virulence and pathogenesis of an amb-resistant isolate of a. terreus (atr) with that of a rare variant showing enhanced sensitivity for amb (ats). the modifications that result in enhanced amb sensitivity of isolates are not associated with reduced virulence in vivo; instead, the ats-infected mice died even faster than the atr-infected animals. ...201323722593
lovastatin in aspergillus terreus: fermented rice straw extracts interferes with methane production and gene expression in methanobrevibacter smithii.lovastatin, a natural byproduct of some fungi, is able to inhibit hmg-coa (3-hydroxy-3 methyl glutaryl coa) reductase. this is a key enzyme involved in isoprenoid synthesis and essential for cell membrane formation in methanogenic archaea. in this paper, experiments were designed to test the hypothesis that lovastatin secreted by aspergillus terreus in fermented rice straw extracts (frse) can inhibit growth and ch4 production in methanobrevibacter smithii (a test methanogen). by hplc analysis, 7 ...201323710454
aspergillus arachnoiditis post intrathecal baclofen pump insertion.this report describes an unusual fungal infection of an intrathecal baclofen pump which, to our knowledge, has not been reported previously. we describe a 39-year-old man with severe lower limb spasticity due to secondary progressive multiple sclerosis that was managed with insertion of an intrathecal baclofen pump. he subsequently presented with distinct neurological decline secondary to an intrathecal baclofen pump infection with aspergillus terreus.201323685108
genome-scale reconstruction and in silico analysis of aspergillus terreus metabolism.aspergillus terreus is widely used for industrial production of important chemical compounds such as itaconate and lovastatin. to improve understanding of its physiology, we reconstructed a genome-scale metabolic model, ijl1454, consisting of 1454 genes, 1451 reactions and 1155 metabolites, based on genome annotation and literature mining. the model accurately predicted the growth phenotype of a. terreus on different carbon and nitrogen sources, and after deletion of essential genes. in ijl1454, ...201323624532
purification and biochemical characterization of feruloyl esterases from aspergillus terreus mtcc 11096.aspergillus terreus mtcc 11096 isolated from the soils of agricultural fields cultivating sweet sorghum was previously identified to produce feruloyl esterases (faes). the enzymes responsible for feruloyl esterase activity were purified to homogeneity and named as atfae-1, atfae-2, and atfae-3. the enzymes were monomeric having molecular masses of 74, 23 and 36 kda, respectively. active protein bands were identified by a developed ph-dependent zymogram on native page. the three enzymes exhibited ...201323606660
biosynthetic pathway for the epipolythiodioxopiperazine acetylaranotin in aspergillus terreus revealed by genome-based deletion analysis.epipolythiodioxopiperazines (etps) are a class of fungal secondary metabolites derived from diketopiperazines. acetylaranotin belongs to one structural subgroup of etps characterized by the presence of a seven-membered 4,5-dihydrooxepine ring. defining the genes involved in acetylaranotin biosynthesis should provide a means to increase the production of these compounds and facilitate the engineering of second-generation molecules. the filamentous fungus aspergillus terreus produces acetylaranoti ...201323586797
a new sesquiterpene from the mangrove endophytic fungus aspergillus terreus (no. gx7-3b).a new compound 1 (named botryosphaerin f), along with other three known compounds 2 (13,14,15,16-tetranorlabd-7-ene-19,6b:12,17-diolide), 3 (botryosphaerin b) and 4 (ll-z1271β), has been isolated from the mangrove fungus aspergillus terreus (no. gx7-3b). the structure of the new compound was established by analysis of spectroscopic data. the hypothetical biogenic relationship of four sesquiterpene analogues was also described in this paper. furthermore, in the cytotoxicity assays, compound 1 sho ...201323521163
aspergillus terreus-related ureteral obstruction in a diabetic aspergillus fungal ball is a rare cause of ureteral obstruction attributed to indwelling catheters, stents, antibiotics, anastomotic leaks, obstruction, and immunosuppressive therapy and other immunocompromised states. we describe a case of unilateral ureteral obstruction caused by aspergillus terreus following ureteroscopic lithotripsy and ureteral stenting in a 45-year-old diabetic man. the patient was successfully treated with endoscopic removal of the fungal mass and oral voriconazole. we ...201323485541
lovastatin-enriched rice straw enhances biomass quality and suppresses ruminal methanogenesis.the primary objective of this study was to test the hypothesis that solid state fermentation (ssf) of agro-biomass (using rice straw as model); besides, breaking down its lignocellulose content to improve its nutritive values also produces lovastatin which could be used to suppress methanogenesis in the rumen ecosystem. fermented rice straw (frs) containing lovastatin after fermentation with aspergillus terreus was used as substrate for growth study of rumen microorganisms using in vitro gas pro ...201323484116
aspergillus terreus endogenous endophthalmitis in a nonimmunocompromised patient with a history of present the first case of aspergillus terreus endophthalmitis in a patient without previous ocular surgery or immunocompromised status with a review of literature.201323480607
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