| targeting highly conserved 3'-untranslated region of pecluviruses for sensitive broad-spectrum detection and quantitation by rt-pcr and assessment of phylogenetic relationships. | the 3'-end region of many virus isolates has been shown to possess conserved sequences in addition to the presence of numerous genomic and subgenomic rnas. utilizing these sequences, a broad-spectrum reverse transcription-polymerase chain reaction protocol has been developed to detect all the known indian peanut clump virus and peanut clump virus isolates, that cause peanut clump diseases in west africa and india. the primers were targeted at the highly conserved 3'-untranslated regions of the p ... | 2010 | 20723565 |
| seed, soil and vegetative transmission contribute to the spread of pecluviruses in western africa and the indian sub-continent. | peanut clump and sugarcane red leaf mottle diseases are caused by viruses of the genus pecluvirus. indian peanut clump virus occurs in the indian sub-continent and peanut clump virus in west africa. a feature of these viruses is that they are both seed and soil transmitted. both modes of transmission contribute to long-term persistence and field spread. data on seed transmission in pearl millet, virus movement within the plant and virus diversity based on rna-1 partial sequences are presented. t ... | 2009 | 19152814 |
| molecular diversity of rna-2 genome segments in pecluviruses causing peanut clump disease in west africa and india. | the complete nucleotide sequence of rna-2 genome segments of four isolates of peanut clump virus (pcv) and two isolates of indian peanut clump virus (ipcv) were determined. comparisons among the complete rna-2 sequences of six isolates from this study and two published earlier, revealed a high degree of variability in size (between 4290 and 4652 nucleotides) and nucleotide sequence identities (between 58 % and 79 %). amino acid sequence alignments of the five open reading frames (orf) showed tha ... | 2003 | 12536297 |
| the nucleotide sequence of indian peanut clump virus rna 2: sequence comparisons among pecluviruses. | the rna-2 molecule of an isolate of the l serotype of indian peanut clump virus (ipcv) was shown to consist of 4,290 nucleotides with five open reading frames (orf). the arrangement of the orfs resembled that in rna-2 of peanut clump virus (pcv) from west africa. the proteins encoded by the orfs in ipcv-l rna are between 32% and 93% identical to those encoded by pcv rna. partial sequence data for the rna-2 of isolates of the h and t serotypes of ipcv show that the coat and p40 proteins encoded b ... | 2000 | 11043946 |
| virus-like particles assemble in plants and bacteria expressing the coat protein gene of indian peanut clump virus. | cdna copies of the coat protein (cp) gene of indian peanut clump virus (ipcv)-h were introduced into cells of nicotiana benthamiana or escherichia coli by transformation with vectors based on prokii or pet respectively. in both plant and bacterial cells, ipcv cp was expressed and assembled to form virus-like particles (vlp). in plant extracts, the smallest preponderant particle length was about 50 nm. other abundant lengths were about 85 and about 120 nm. the commonest vlp length in bacterial ex ... | 2000 | 10640566 |
| genome sequences of poa semilatent and lychnis ringspot hordeiviruses. | the nucleotide sequences of the genomic rnas of two hordeiviruses, poa semilatent virus (pslv) and lychnis ringspot virus (lrsv), were determined. the genome organization of both viruses is similar to that of the type hordeivirus, barley stripe mosaic virus (bsmv). comparisons of the amino acid sequences of the hordeivirus replicase components revealed a high level of sequence similarity to those of soil-borne wheat mosaic virus (sbwmv), peanut clump virus (pcv), and indian peanut clump virus. t ... | 1998 | 9722881 |
| transfer rna mimicry in a new group of positive-strand rna plant viruses, the furoviruses: differential aminoacylation between the rna components of one genome. | recent sequencing of the genomes of several furoviruses--fungus-transmitted rod-shaped positive-strand plant viruses--has suggested the presence of trna-like structures (tlss) at the 3' ends of the genomic rnas. we show here that the genomic rnas of soil-borne wheat mosaic virus (sbwmv), beet soil-borne virus (bsbv), potato mop-top virus (pmtv), peanut clump virus (pcv), and indian peanut clump virus (ipcv) all possess functional tlss that are capable of high-efficiency valylation. while the sbw ... | 1998 | 9657004 |
| the nucleotide sequence of rna-1 of indian peanut clump furovius. | the nucleotide sequence of rna-1 of an isolate of the h serotype of indian peanut clump virus (ipcv-h) was shown to comprise 5,841 nucleotides. the rna contains three open reading frames (orf) which are between nucleotides 133 and 3,522, nucleotides 3,526 and 5,103 (assuming expression by suppression of the orf 1 termination codon) and nucleotides 5,168 and 5,539. the encoded polypeptides have m(r), of 129,687 (p130), 60,188 (p60) and 14,281 (p14). orf 2 is thought to be expressed by suppression ... | 1996 | 9526538 |
| the coat protein of indian peanut clump virus: relationships with other furoviruses and with barley stripe mosaic virus. | the 5'-most open reading frame of the c.4kb rna-2 of indian peanut clump furovirus (ipcv) encodes a protein of 208 amino acids. this protein is thought to be the coat protein of ipcv because its amino acid composition and m(r) closely resemble those reported for ipcv coat protein and because its amino acid sequence is 61% identical to that of the coat protein of peanut clump virus (pcv) from west africa. the extent of the sequence identity between ipcv and pcv coat proteins confirms previous con ... | 1994 | 8129616 |
| an efficient method for the production of transgenic plants of peanut (arachis hypogaea l.) through agrobacterium tumefaciens-mediated genetic transformation. | cotyledon explants from mature peanut seeds (arachis hypogaea l.) were optimized to obtain adventitious shoot buds with high frequencies (>90%). efficient transformation of these cotyledons by using agrobacterium tumefaciens strain c58 carrying neomycin phosphotransferase ii (nptii) and ß-glucuronidase (gus; uida), or coat protein gene of the indian peanut clump virus (ipcvcp) and nptii on binary vectors (pbi121; prokii:ipcvcp) led to the production of a large percentage (55%) of transgenic plan ... | 2000 | 11011088 |