| purification and isolation of a biologically active peptido-rhamnogalactan from sporothrix schenckii. | | 1976 | 45715 |
| serologic differences in strains of sporothrix schenckii. | to obtain evidence that sporothrix scheneckii enters the body by contact with contaminated materials, the antigenic property of strains from different sources was investigated. the reciprocal absorption test of the antisera against a soil isolate and a human isolate (ko 4606) showed that the absorbed antisera against ko 4606 possessed unique antigen(s) in addition to the common antigen of both strains. twenty-three clinical isolates were tested with absorbed antisera. not all of them possessed t ... | 1975 | 57646 |
| delayed hypersensitivity cross-reactions between sporothrix schenckii and ceratocystis species in sporotrichotic patients. | cutaneous delayed hypersensitivity to antigens prepared from sporothrix schenckii and several ceratocystis species, including c. stenoceras, c. ulmi, c. ips, and c. minor, was tested in 14 patients with known cutaneous sporotrichosis. extensive cross-reactions were observed. nonsporotrichotic people (controls) did not react to these antigens. the correlation coefficient between antigens of s. schenckii and each ceratocystis species was calculated from the areas of the cutaneous reactions. among ... | 1976 | 59734 |
| immunoperoxidase localization of sporothrix schenckii and cryptococcus neoformans. staining of tissue sections fixed in 4% formaldehyde solution and embedded in paraffin. | an indirect immunoperoxidase staining method has been successfully applied to 4% formaldehyde solution tissue sections fixed in and embedded in paraffin for the localization of sporothrix schenckil and cryptococcus neoformans without prior trypsinization of tissue sections. a comparison of this method with an analogous immunofluorescence staining technique has been made. | 1979 | 86325 |
| distribution of anionic groups at the cell surface of different sporothrix schenckii cell types. | the distribution of anionic groups at the cell surface of yeastlike forms, hyphae, and conidia of sporothrix schenckii was studied by staining with colloidal iron hydroxide and cationized ferritin. by using colloidal iron hydroxide it was shown that the external cell wall layer of one strain (strain 1099.18) could be resolved into two reactive sublayers and that these layers were present in many but not all cells of the same population. in contrast, most cells of another strain (strain 1099.12) ... | 1979 | 89092 |
| serological cross-reactivity among sporothrix schenckii, ceratocystis, europhium, and graphium species. | ethanol-precipitable culture filtrate antigens of 100 strains of 75 species of the sporothrix-ceratocystis-europhium-graphium complex and 1 species of botrytis were examined for neutral sugar components and for serological cross-reactivity with s. schenckii rabbit antiserum and human sporotrichosis sera by capillary precipitin and double immunodiffusion assay. results revealed that cross-reactive species (60 of 77, ca. 80%) produced exoconidial forms and rhamnose- and mannose-containing polysacc ... | 1978 | 99369 |
| immunochemical studies on the human pathogen sporothrix schenckii: effects of chemical and enzymatic modification of the antigenic compounds upon immediate and delayed reactions. | the rhamnose-containing polysaccharide-peptide compound derived from the cells of the pathogenic fungus sporothrix schenckii has been shown to contain 87.1% carbohydrate and 12.5% peptide and to give rise to both immediate- and delayed-type reactions in sensitized guinea pigs. the capacity to induce immediate-type reaction, passive cutaneous anaphylaxis, was completely lost by degradation of the carbohydrate moiety by periodate, whereas the ability to induce the delayed-type reactions of migrati ... | 1975 | 166914 |
| cerebrospinal fluid lymphocyte transformations in meningitis. | cerebrospinal fluid lymphocytes from 13 patients with nonsuppurative meningitis were cultured with antigens derived from mycobacterium tuberculosis, sporotrichum schenckii, and herpes simplex. when csf lymphocytes from five patients with infections associated with these organisms were incubated with "correct" antigen there was increased incorporation of thymidine. the levels were higher than those seen when the cells were incubated with different antigens or when csf lymphocytes from patients wi ... | 1979 | 219789 |
| phagocytosis and intracellular fate of sporothrix schenckii. | human polymorphonuclear leukocytes (pmnls) phagocytized and killed yeast-phase cells of sporothrix schenckii in vitro in the presence of 10% unheated serum. the combination of h2o2, ki, and human pmnl myeloperoxidase was lethal to yeast-phase s. schenckii. the results of this investigation suggest that pmnls may play an important role in host resistance to infection with s. schenckii. | 1979 | 231073 |
| primary pulmonary sporotrichosis. | a 34-year-old alcoholic and drug addict developed cavitary pulmonary sporotrichosis that progressed slowly during 6 years. pulmonary resection and pre- and postoperative therapy with amphotericin b were associated with prompt clinical improvement with no evidence of relapse during a 2-year follow-up. histologic examination of lung revealed granulomatous inflammation with organisms consistent with sporothrix schenckii, and interstitial talc (magnesium silicate) granulomas. the latter finding was ... | 1977 | 262099 |
| serological cross-reactivity between group b streptococcus and sporothrix schenckii, ceratocystis species, and graphium species. | serological cross-reactivity of a group b streptococcus (h36b) with sporothrix schenckii and 39 different ceratocystis and graphium species was investigated by double immunodiffusion. rabbit anti-h36b serum reacted with antigens from s. schenckii and from 36 of 39 ceratocystis and graphium species. it is speculated that low-titer agglutinins to s. schenckii in normal sera are due to antibodies raised against various bacteria which share common antigens with s. schenckii. | 1977 | 324913 |
| merthiolate treatment of pathogenic fungi. | the action of merthiolate on the pathogenic yeasts blastomyces, dermatitidis, histoplasma capsulatum, and sporothrix schenckii was compared to the effect of treatment with formaldehyde. concentrations of 1:10,000 and 1:5,000 merthiolate for three exposure times (24, 48, and 72 h) at 4 and 25 degrees c were tested on three media (brain heart infusion with and without blood, and modified sabouraud agar). the effect of merthiolate on these three yeasts was primarily fungistatic, with maximum effect ... | 1979 | 389944 |
| experimental sporotrichosis in syrian hamsters. | syrian hamsters were infected with sporothrix schenckii by subcutaneous footpad inoculation. two types of infection could be uniformly induced: a self-limited, lymphatic infection resembling the classical disease in humans, and a generalized nonfatal infection. an infecting dose of approximately 5,300 yeast cells produced the localized subcutaneous-lymphatic disease which was limited to a single limb. in contrast, a 1,000-fold increase in the inoculum temporarily overwhelmed the animals' defense ... | 1979 | 422244 |
| comparison of isolates of sporothrix schenckii obtained from fixed cutaneous lesions with isolates from other types of lesions. | | 1979 | 438543 |
| disseminated sporotrichosis in a cat. | sporothrix (sporotrichum) schenckii was identified as the causative agent of a large pyogranulomatous lesion on the right forepaw of a cat. organisms also were observed in axillary lymph nodes of the affected forelimb and in the lungs and liver of the cat. the agent was transmitted to another domestic cat and to mice. | 1979 | 500443 |
| auricular sporotrichosis in a brick mason. | a case of cutaneous sporotrichosis involving the auricle of a brick mason is reported. despite the infrequency of such an infection, sporotrichosis must be considered in the differential diagnosis of perichondritis. the occupational associations with sporothrix schenckii are reviewed and the relationship with bricks is emphasized. | 1979 | 507872 |
| [an endemic area of sporotrichosis in guatemala]. | an endemic area of sporotrichosis is described in the lake of ayarza district, south guatemala, where 53 patients have been observed within 3 years. in 45.3% of the cases, the infection appeared after handling fish. the disease was more frequently observed in man (83%) and in patients less than 30 years of age. the most frequent clinical type was the ascending lymphocutaneous sporotrichosis of the limbs. some cases of ulcerative or verrucous lesions were seen. almost all the patients cured rapid ... | 1978 | 360441 |
| further studies on the rhamnomannans and acidic rhamnomannans of sporothrix schenckii and ceratocystis stenoceras. | | 1977 | 558825 |
| cutaneous sporotrichosis in forestry workers. epidemic due to contaminated sphagnum moss. | in december 1975 and january and february 1976, an epidemic of cutaneous sporotrichosis occurred in mississippi among forestry workers and other persons exposed to sphagnum moss used in packing pine seedlings. seventeen cases were identified, 15 of which were from patients who had been exposed to sphagnum moss from a single source. attack rates were significantly higher among workers exposed to this moss than among those not exposed. sporothrix schenckii was cultured from the implicated batch of ... | 1978 | 566336 |
| therapeutic failures with miconazole. | a retrospective review of therapeutic failures of miconazole in three patients is presented. miconazole, a new imidazole derivative, is a broad-spectrum antifungal agent purportedly effective topically, orally, and parenterally against a number of species of fungi. three patients with the following culturally proven deep fungal infections were treated with miconazole: (i) destructive arthritis (sporothrix schenckii), (ii) meningoencephalitis (cryptococcus neoformans), and (iii) disseminated aspe ... | 1978 | 354523 |
| laboratory-acquired sporotrichosis. | a case of laboratory-acquired sporotrichosis was diagnosed by immunofluorescence and by culture. the patient was then cured by treatment with oral potassium iodide. the need to handle cultures of sporothrix schenckii with due caution is stressed. | 1977 | 333622 |
| spontaneous feline sporotrichosis: a fine structural study. | fine structural details of the parasitic yeastlike phase of sporothrix schenckii contained in biopsy tissue from a naturally-occurring case of disseminated feline sporotrichosis are described and illustrated by electron microscopy. both free and phagocytosed fungal cells were observed. the fungal cells were contained within an extracellular, electron transparent vacuolar area which was bounded by a limiting membrane of probable host origin. the yeastlike cells were characterized by a conspicuous ... | 1979 | 542207 |
| [tropic-subtropic fungus infections in germany]. | observations by the author and other workers on exotic mycoses in germany are used in order to establish several groups of patients. the following mycoses from tropical and subtropical areas and their causative agents are mentioned: tinea nigra (cladosporium werneckii), south american blastomycosis (paracoccidioides brasiliensis), coccidioidomycosis (coccidioides immitis), histoplasmosis (histoplasma capsulatum), mycetoma (madurella mycetomi), dermatophytosis/tinea capitis (trichophyton soudanen ... | 1978 | 627480 |
| taxonomy of exophiala jeanselmei (langeron) mcginnis and padhye. | the black yeast-like fungus exophiala jeanselmei (langeron) mcginnis et padhye was originally described as torula jeanselmei by langeron. since its original description in 1928, this dematiaceous hyphomycete has been placed in the genera phialophora, pullularia and rhinocladiella by various authors. study of the type culture of e. jeanselmei has confirmed that the principle conidiogenous cells of this fungus are annellides, thus supporting its current generic disposition. after reviewing the neo ... | 1978 | 570642 |
| chronic meningitis caused by sporotrichum schenckii. | the fungus sporotrichum schenckii caused chronic meningitis in a 48-year-old man. only three other firmly diagnosed cases were reported previously. | 1977 | 561914 |
| in vitro studies with ambruticin, a new antifungal antibiotic. | the in vitro antifungal inhibitory activities of ambruticin and of various antifungal drugs of choice against 190 fungal pathogens representative of the major human mycoses were compared using a modification of the ics agar dilution technique. ambruticin compared favorably with amphotericin b and miconazole when tested against the dimorphic pathogens coccidioides immitis, histoplasma capsulatum, and blastomyces dermatitidis and against aspergillus fumigatus. miconazole was the most active compou ... | 1978 | 686712 |
| ultrastructural observations of an unusual osmiophilic body in the hyphae of sporothrix schenckii and ceratocystis stenoceras. | electron microscopy of 8 strains of sporothrix schenckii and 1 strain each of ceratocystis stenoceras, c. pluriannulata, c. ulmi, and c. minor revealed the presence of unusual osmiophilic structures (eob) which appeared as normal organellar components of young cells of these fungi. in s. schenckii and c. stenoceras, these structures were markedly osmiophilic, reacted strongly with thiocarbohydrazide, could be partially solubilized with the lipid solvent sodium methoxide, and appeared to possess ... | 1977 | 603192 |
| pulmonary sporotrichosis in oklahoma in susceptibilities in vitro. | six cases of pulmonary sporotichosis were observed in 2 institutions in oklahoma city, okla. three of the patients were treated with iodides with or without surgery. although one patient required a second course of iodides, the patients have remained well after at least 34 months of follow-up. three patients treated with amphotericin b, single course as well as multiple courses, and other antifungal agents (hydroxystilbamidine and miconazole) have all relapsed. these cases and a reviewed of more ... | 1979 | 582240 |
| sporotrichosis in a pacific white-sided dolphin (lagenorhynchus obliquidens). | a severe necrotizing granulomatous lymphadenitis caused by sporothrix schenckii was diagnosed in a pacific white-sided dolphin (lagenorhynchus obliquidens) that had been in captivity for about 3 years. histopathologic and electron microscopic studies, as well as fluorescent antibody techniques, were used to identify s schenckii as the etiologic agent. | 1978 | 749575 |
| existence of sporothrix schenckii as a pulmonary saprophyte. | a 48-year-old male custodian and part-time gardener was hospitalized for treatment of renal tuberculosis. sputum cultures failed to reveal mycobacteria, but sporothrix schenckii was isolated over an eight-month period. in the absence of clinical or roentgenographic evidence of active lung disease, we postulate the saprophytic existence of s schenckii in this patient's tracheobronchial tree. recognition of this state would obviate the need for the commonly accepted therapy for pulmonary sporotric ... | 1978 | 630944 |
| atigenic similarity between ceratocystis species and sporothrix schenckii as observed by immunofluorescence. | antigenic properties of sporothrix schenckiii and 2 species of ceratocystis (c. stenoceras and c. ulmi) were compared by indirect immunofluorescent staining technique. both species of ceratocystis and 2 strains of s. schenckii reacted equally well with the unabsorbed antisera against 2 strains of s. schenckii, indicating that these three species share common antigen. the absorption experiments showed that only s. schencki human strain demonstrated a unique antigen. these results support the hypo ... | 1976 | 785629 |
| immunochemical studies on l-rhamno-d-mannans of sporothrix schenckii and related fungi by use of rabbit and human antisera. | antisera were prepared in rabbits against the human pathogenic yeast sporothrix schenckii (strain 1099.12) grown at two different temperatures (25 degrees and 37 degrees). precipitation and inhibition data showed that the former serum had a specificity directed against alpha-l-rhap-(1 yields 2)-alpha-l-rhap-(1 yields 3)-d-man-(1 yields determinants, whereas the latter had a broad specificity in which alpha-l-rhamnosyl or alpha-l-rhap-(1 yields 3)-d-man- was the immunodominant structure. these re ... | 1975 | 804993 |
| heterogeneity of the rhamnomannans from one strain of the human pathogen sporothrix schenckii determined by 13c nuclear magnetic resonance spectroscopy. | the synthesis of different rhamnomannans in a strain of sporothrix schenckii (1099.12) was shown by use of 13c nuclear magnetic resonance spectroscopy. fractionation of a polysaccharide preparation from cells grown at 25 degrees c provided a neutral monorhamnosyl rhamnomannan and an acidic rhamnomannan containing 4-o-substituted glucuronic acid units and also (1 leads to 2)-linked dirhamnosyl side chains. | 1978 | 640728 |
| spore ultrastructure in sporothrix schenckii. | pathogenic strains of sporothrix schenkii may show triangular spores, whose angular shape is maintained by a tie-beam effect in the inner cell wall structure. this difference in wall structure lies adjacent to a folded and possibly more active part of the spore cytoplasm. the supposed generation of asci in old cultures was simulated by the death of hyphae which are reinvaded by intrahyphal growth with intrahyphal spore production, while true asci were not seen. | 1979 | 530299 |
| synthesis of monorhamnosyl l-rhamno-d-mannans by conidia of sporothrix schenckii. | a rhamnomannan containing single-unit alpha-l-rhamnopyranose side chains was identified in isolated conidia from sporothrix schenckii. such a rhamnomannan differed from the dirhamnosyl rhamnomannan synthesized by the hyphae but was very similar to the monorhamnosyl rhamnomannan formed in yeastlike cells. nuclear magnetic resonance spectroscopy and chemical analysis were used to compare these polysaccharides. based on the distribution of different rhamnomannans in different s. schenckii cell type ... | 1978 | 624583 |
| sporotrichosis: an unusual disseminated cutaneous case and a fatal pulmonary case. | two unusual cases of sporotrichosis are presented. one patient had disseminated vesicular skin lesions which yielded sporothrix schenckii on culture. the other, with extensive pulmonary sporotrichosis, continued to have sputum cultures positive for s schenckii during three years of intensive chemotherapy. | 1976 | 941052 |
| conidiogenesis in pathogenic hyphomycetes. i. sporothrix, exophiala, geotrichum and microsporum. | conidium formation in 5 species of pathogenic hyphomycetous fungi, sporothrix schenckii, exophiala salmonis, e. sp., geotrichum candidum and microsporum canis were examined using light and scanning electron microscopy. the fungi, grown in pure culture on potato dextrose agar under defined conditions, demonstrated 4 kinds of conidiogenesis. the taxonomic value of the developmental characters in the classification of the fungi imperfecti is discussed. | 1976 | 944466 |
| long-chain fatty acids of sporothrix (sporotrichum) schenckii. | a number of strains purporting to belong to the species sporothrix schenckii were examined for their fatty acid content. the majority of the strains were isolated from cases of sporotrichosis. two strains were reputedly saprophytic. in all cases except the two saprophytic ones the major fatty acid was a c18 diene. considerable amounts of palmitic acid and c18 monoene were found in all strains. | 1976 | 950381 |
| isolation of sporothrix schenckii in the soil in israel in relation to a new case in man. | a new case, the third in israel, of subcutaneous sporotrichosis is presented. a pigmentary strain of sporothrix schenckii was cultured from the unopened and necrotic nodules of the left hand and forearm of an 80-year-old male. experimental inoculation of the isolated fungus into mice led to the dissemination of the organism in the liver, kidneys and lungs inoculated intraperitoneally, and the development of orchitis with abscess formation in those inoculated intratesticularly. in both groups of ... | 1976 | 959947 |
| [oxygen consumption by the yeast-like and filamentous forms of sporothrix schenckii as measured by polarography]. | the study of the oxygen uptake by cultures of sporothrix schenckii as measured with the clark electrode has shown that when the fungus was grown in a liquid medium, the atmospheric oxygen went into solution very slowly even when the liquid was rapidly stirred. the partial oxygen pressure was very small after some days of culture (no more than 2 or 3% expressed as the saturated value). hence, it is postulated that the linear part of the growth curve is due to the dissolved oxygen acting as a limi ... | 1976 | 816527 |
| skin test and blastogenic responses to sporotrichun schenckii. | in vivo skin testing and in vitro lymphocyte blastogenesis were evaluated in a young adult population as methods for detecting cellular immunity to sporotrichum schenckii. similar procedures for candida albicans and coccidioides immitis were also investigated. 5 of 143 subjects had positive skin tests and 14 had positive blastogenic responses to s. schenckii. these 14 subjects also exhibited unusually high responses to c. albicans in vitro and 11 of the 14 were female. data demonstrated a correl ... | 1976 | 1107353 |
| [ecological and epidemiological relationships between sporothrix schenckii, a fungus pathogenic for man and the ceratocystis genus]. | sporothrix schenckii is a pathogenic fungus for man and animal. its perfect form is not yet known. many studies have been made to compare this fungus to ascomycetes belonging to the genus ceratocystis. this paper summarizes some important data about the ecology and the epidemiology of the fungal complex sporothrix-ceratocystis. previous results obtained by several groups of workers are strongly in favor of a relationship between the two genera. the epidemiological studies carried out in differen ... | 1978 | 743769 |
| comparative in vitro antifungal activity of amphotericin b and amphotericin b methyl ester. | the in vitro antifungal activity of amphotericin b methyl ester (ame), a water-soluble derivative of amphotericin b, was compared to that of the parent compound against a variety of pathogenic and potentially pathogenic fungi. ame has a significant antifungal activity, but the activity of ame was slightly lower than that of amphotericin b. among the yeast-like organisms, only the yeast cells of sporothrix schenckii were more resistant than others to both antibiotics, with a minimal fungicidal co ... | 1975 | 1137359 |
| the combined effect of a cutaneo-lymphatic fungus, sporothrix schenckii and a lymphatic-dwelling nematode, brugia malayi. | domestic cats were infected with third-stage brugia malayi in such a way that the parasites were restricted to the regional lymphatics of one hind limb. later, these cats were exposed on the same leg to the yeast phase of sporothrix schenckii. edema and fibrosis were more extensive in cats infected with both brugia and sporothrix than in cats with either of these organisms alone. lesions tended to appear earlier, more consistently and progressed more rapidly in cats with dual infections than in ... | 1977 | 883012 |
| ex vivo determination of potentially virulent sporothrix schenckii,. | hyphae from 30 isolants of sporothrix and ophiostoma species were washed, dried and pyrolyzed at 350 degrees c. pyrolysis products were separated on a carbowax column heated 7.5 degrees c/min to and maintained for 50 min at 160 degrees c. hydrogen flame detector responses were recorded graphically. fifteen clinical isolants of s. schenckii from geographically separated sources produced qualitatively identical pyrograms. s. foliorum, 8 avirulent s. schenckii and other sporothrix species isolants ... | 1976 | 934262 |
| granulomatous synovitis and osteitis caused by sporothrix schenckii. | sporotrichosis must be considered in the differential diagnosis of granulomatous inflammation involving bones and joints. the organisms are difficult to demonstrate in direct smears and in histiologic sections, but they grow readily on routine fungal culture media. the cases of two patients, one with sporothrix arthritis and one with sporothrix arthritis and osteitis, are presented. the latter patient underwent ten surgical procedures over a period of 6 1/2 years and was treated for tuberculous ... | 1975 | 1163487 |
| sensitivity of some human pathogenic yeasts and systemic fungi to myxin. | myxin, a relatively new antibacterial and antifungal antibiotic, produced by a species of sorangium, was used to investigate its effectiveness against some yeasts and dimorphic fungi associated with human diseases. results indicated that the minimal fungicidal concentrations (mfc) of myxin for candida albicans, c. krusei, c. parapsilosis, c. tropicalis, and torulopsis glabrata were 0.39-6.25 mug/ml, and for c. guilliermondii and c. tropicalis 12.5-25 mug/ml. the mfc for blastomyces dermatitidis ... | 1975 | 1171116 |
| [occurrence of neuraminidase in sporothrix schenckii and ceratocystis stenoceras and its role in ecology and pathomechanism of these fungi (author's transl)]. | some strains of sporothrix schenckii and one strain of ceratocystis stenoceras were investigated and the occurrence of the enzyme neuraminidase (= sialidase, n-acetylneuraminate glycohydrolase, ec 3.2.1.18) was proved not only by warren's thiobarbituric acid assay but also by immunoelectrophoresis technique using monospecific antisera against 25 human glycoproteins, lipoproteins, immunoglobulins, fibrinogen and some other proteins (table 1). there are also fibrinolytic and lipolytic enzyme activ ... | 1975 | 1179886 |
| ultrastructural studies of the mycelium-to yeast transformation of sporothrix schenckii. | fine details of the internal and external morphology of the in vitro mycelial phase (mp) to yeastlike phase (yp) transition of the dimorphic fungal pathogen sporothrix schenckii are shown in electron micrographs of ultrathin sections. morphological transformation at the ultrastructural level was observed to occur by direct formation of budlike structures at the tips and along the hyphae and by oidial cell formation. direct budding of yeast from conidiospores was not observed. early transitional ... | 1975 | 1184578 |
| effect of cetylpyridinium chloride on pathogenic fungi and nocardia asteroides in sputum. | the effect of cetylpyridinium chloride (cpc) on pathogenic fungi and nocardia asteroides was studied. sputa seeded with each of 11 organisms (aspergillus flavus; aspergillus fumigatus, blastomyces dermatitidis, candida albicans, coccidioides immitis, cryptococcus neoformans, geotrichum candidum, histoplasma capsulatum; nocardia asteroides, paracoccidioides brasiliensis, and sporothrix schenckii) were treated with cpc and kept for 2, 5 and 9 days. the cpc reagent used (0.5% cpc and 0.5% sodium ch ... | 1976 | 773957 |
| [ecology of sporothrix schenckii and of ceratocystis stenoceras in corsica and alsace, french provinces free of sporotrichosis]. | the study was conducted in 2 french provinces free of sporotrichosis. of 40 samples (soil, plant fragments, . . .) taken from corsica, 59 isolations were obtained. all of these contained s. schenckii type of growth. of 30 retained for further study, 14 were later identified as ceratocystis stenoceras. all 14 needed pyrimidine as a growth factor. they developed well at 37 degrees and gave a yeast-like growth in shaken medium at 35 degrees and 25 degrees. one of the strains was pathogenic to mice. ... | 1975 | 1162543 |
| morphological conversion of 13 strains of three dimorphic fungi in tap and stream water. | various strains of sporotrichum schenckii, histoplasma capsulatum and paracoccidioides brasiliensis show partial to complete (ca 70%) conversion to yeast when the mycelial culture is grown on sabouraud's dextrose agar at 25degreesc and palced in tap or stream water at 37degreesc. conversion (ca 70%) to mycelial phase occurs when the yeast culture is grown on brain heart infusion agar at 37degreesc and then placed in tap or stream water at 25degreesc. it was observed that, with the dimorphic orga ... | 1975 | 1242119 |
| [fatty acid composition of polar and neutral lipids of sporothrix schenckii and ceratocystis stenoceras]. | the fatty acids contained in the neutral and polar lipids from sprorthrix schenckii and ceratocystis stenoceras and 3 mutants of the latter fungus were found to be identical. the major fatty acids were palmitic, oleic and linoleic. the fungi had similar quantitative composition especially the mutant strains and s. schenckii. this observation provides more data regarding the possible relationship between ceratocystis and sporothrix. | 1975 | 1162544 |
| new azasteroidal antifungal antibiotics from geotrichum flavo-brunneum. iii. biological activity. | the a25822 antibiotic complex consists of seven biologically active factors. a comparative study of these factors determined that factor b possessed the greatest antifungal activity. the minimal inhibitory concentration of a25822b against isolates of candida albicans was less than 0.3 similar to 5.0 mug/ml, trichophyton mentagrophytes was inhibited at less than 0.0312 mug/ml. other pathogenic fungi such as cryptococcus neoformans, histoplasma capsulatum, blastomyces dermatitidis, sporotrichum sc ... | 1975 | 1089623 |
| in vitro antifungal spectrum of itraconazole and treatment of systemic mycoses with old and new antimycotic agents. | itraconazole is a lipophilic triazole with potent in vitro activity. it is also effective after topical, oral and parenteral administration. the antifungal activity of itraconazole has been evaluated against more than 6,500 different strains, belonging to more than 260 fungal species, using the serial decimal dilution test in fluid broth medium (brain-heart infusion broth). candida spp., torulopsis spp., cryptococcus neoformans, pityrosporum spp. (dixon broth), various other yeasts, dermatophyte ... | 1992 | 1319313 |
| convergence in ascospore discharge mechanism among pyrenomycete fungi based on 18s ribosomal rna gene sequence. | fungi of the class pyrenomycetes (ascomycotina) form a morphological series ranging from those that shoot ascospores (sexual spores) forcibly from the ascus (spore sac) to fungi that ooze ascospores or have no obvious mechanism for ascospore release. did forcible ascospore discharge evolve within these pyrenomycetes, or has it been lost in the group? we determined the sequences of the 18s ribosomal rna gene from three fungi and used these, along with six sequences from our previous work and thre ... | 1992 | 1342925 |
| o-glycosidically linked oligosaccharides from peptidorhamnomannans of sporothrix schenckii. | beta-elimination of peptidorhamnomannans purified from yeast-like and mycelial phases of sporothrix schenckii released neutral and acidic reduced oligosaccharides that were o linked to serine and/or threonine. man-(alpha 1-2)man-ol, rha(alpha 1-3)man(alpha 1-2)man-ol, rha(alpha 1-4)glca(alpha 1-2)man(alpha 1-2)man-ol, and rha(alpha 1-4)[rha(alpha 1-2)] glca(alpha 1-2)man(alpha 1-2)man-ol were characterized based on methylation analysis, proton magnetic resonance and fast atom bombardment mass sp ... | 1992 | 1344711 |
| dna typing of isolates associated with the 1988 sporotrichosis epidemic. | dna typing techniques were used to examine selected clinical and environmental isolates of sporothorix spp. recovered from the 1988 sporotrichosis epidemic in multiple states of the united states. previous studies indicated that isolates in one of the six morphologically or physiologically distinct groups (group i) obtained from environmental sources were sporothrix schenckii and were the possible etiologic agents responsible for the epidemic. to assess this hypothesis at the genetic level, whol ... | 1992 | 1352783 |
| immunohistologic diagnosis of systemic mycoses: an update. | fluorescent antibody, immunoperoxidase and gold-silver staining methods for the rapid and accurate diagnosis of systemic mycotic infections are currently performed in a few specialized laboratories. these methods have proved applicable to formalin-fixed, paraffin-embedded tissues, and are reliable for identifying therein antigens of infectious dimorphic, monomorphic filamentous, and yeast-like fungal pathogens, i.e., aspergillus spp., blastomyces dermatitidis, candida spp., coccidioides immitis, ... | 1992 | 1397200 |
| fatal pulmonary sporotrichosis caused by sporothrix schenckii var. luriei in india. | the first case of fatal pulmonary sporotrichosis caused by sporothrix schenckii var. luriei in a patient from the northwestern region of india is described. in the absence of cultures, the diagnosis was suspected by notation, in lung tissue, of large, thick-walled, hyaline fungal cells that divided internally by septation or a budding process. the thick-walled, internally septated cells often became muriform. the presence of an "eyeglass" configuration of incompletely separated cells characteris ... | 1992 | 1401023 |
| polymorphism of sporothrix schenckii surface polysaccharides as a function of morphological differentiation. | the alkali-extractable polysaccharides from different morphological types of two sporothrix schenckii strains (1099.12 and 1099.18) were investigated. dissociation of morphological phase transition and temperature effects was possible in a synthetic medium which produced cultures with 100% yeast forms either at 25 or at 37 degrees c. only rhamnomannans with single-unit alpha-l-rhamnopyranosyl side chains were formed by the yeast forms irrespective of the incubation temperature. the higher tempe ... | 1976 | 1276152 |
| long-time survival and morphological stability of preserved sporothrix schenckii strains. | the survival and stability of morphological traits of 33 sporothrix schenckii strains were evaluated. strain subcultures were maintained under mineral oil for different periods of time lasting as long as 41 years, or in sterile distilled water for as long as 23 years. of the 33 strains preserved under mineral oil, 28 (85%) maintained viability and unchanged macro- and microscopic characteristics. all of the 4 strains maintained in distilled water also remained viable, with no morphological chang ... | 1992 | 1287484 |
| rapid conversion of histoplasma capsulatum, blastomyces dermatitidis and sporothrix schenckii in tissue culture. | a simple method for positive identification of histoplasma capsulatum, blastomyces dermatitidis, and sporothrix schenckii is given. primary tissue cultures of guinea pig peritoneal macrophage were inoculated with the mycelial phase of each organism and after 24 h the cells were stained and observed microscopically. the characteristic yeast phase could then be observed allowing for positive identification. | 1975 | 1236872 |
| sporothrix schenckii meningitis in a patient with aids. | | 1992 | 1420699 |
| comparison between histochemical and immunohistochemical methods for diagnosis of sporotrichosis. | to compare the efficacy of histochemical and immunohistochemical methods in detecting forms of sporothrix schenckii in tissue. | 1992 | 1479036 |
| role of cell-mediated immunity in the resistance to experimental sporotrichosis in mice. | congenitally athymic nude (nu/nu) mice showed higher sensitivity to intratestical infection of sporothrix schenckii than phenotypically normal littermates (nu/+). active immunization with viable cells enhanced the resistance to intravenous (i.v.) infection of this fungus in balb/c mice. nu/nu mice transferred with immune spleen cells acquired the enhancement of resistance to the infection with s. schenckii, but not ones with normal spleen cells. pre-treatment of ok-432 (picibanil), one of the ma ... | 1992 | 1480205 |
| effects of calcium ions on the germination of sporothrix schenckii conidia. | sporothrix schenckii conidia were induced to germinate in varying concentrations of added calcium. calcium had a stimulatory effect on the process and a calcium concentration of 1 mm was found to be optimal. measurements of radioactive calcium uptake confirmed that uptake was taking place during the germination of conidia to the mycelial form of the fungus. three calcium uptake peaks were observed, the first occurred 10 min after inoculation and the other two peaks coincided with the first 2 cyc ... | 1992 | 1517957 |
| sporothrix schenckii scleritis. | | 1992 | 1524134 |
| sporothrix schenckii fungemia without disseminated sporotrichosis. | fungemia is a rare complication of sporothrix schenckii infection and has always been associated with disseminated sporotrichosis. we describe an immunocompetent patient with localized lymphocutaneous sporotrichosis from whose blood the fungus was isolated. a lysis-centrifugation blood culture system may have improved our ability to detect low-level s. schenckii fungemia. | 1992 | 1537924 |
| use of a mouse model to evaluate clinical and environmental isolates of sporothrix spp. from the largest u.s. epidemic of sporotrichosis. | five clinical and 69 environmental isolates from the largest u.s. epidemic of sporotrichosis were evaluated in nylar male mice following intravenous injection of 5 x 10(6) to 2 x 10(8) conidia per mouse. the clinical isolates and eight environmental isolates produced 100% mortality in groups of three mice each between 12 and 24 days after injection. these virulent isolates grew at 37 degrees c, were dematiaceous by virtue of melanin (melanized) on permissive media (e.g., potato dextrose agar), p ... | 1992 | 1572983 |
| a multistate outbreak of sporotrichosis associated with sphagnum moss. | in the spring of 1988, the largest documented us outbreak of cutaneous sporotrichosis to date occurred, with 84 cases among persons from 15 states who were exposed to wisconsin-grown sphagnum moss used in packing evergreen tree seedlings. in new york state, 13 cases occurred among 109 forestry workers. all 13 cases occurred among 76 workers who had handled evergreen seedlings and moss (attack rate = 17%). for those exposed to evergreens and moss, the risk of infection increased as worktime expos ... | 1992 | 1415167 |
| filamentous forms of sporothrix schenckii in material from human lesions. | this paper reports the observation of the mycelial form of sporothrix schenckii, including conidia, in dried-up purulent material from a cutaneous lesion of the face of a patient with sporotrichosis of over a year's duration. | 1992 | 1469543 |
| [in vitro antifungal activity of itraconazole, a new triazole antifungal agent, against clinical isolates from patients with dermatomycoses]. | in vitro antifungal activities of itraconazole (itz), a triazole antifungal agent, against clinical isolates obtained from patients with superficial and subcutaneous mycoses were examined using the agar dilution method on casitone agar. the clinical isolates tested were 7 species and 263 isolates including trichophyton mentagrophytes (104 isolates), trichophyton rubrum (103 isolates), microsporum canis (3 isolates), epidermophyton floccosum (2 isolates), candida albicans (32 isolates), malassezi ... | 1991 | 1652655 |
| evaluation of new antifungal drugs for the treatment of systemic fungal infections. infectious diseases society of america and the food and drug administration. | these guidelines are applicable to all fungal pathogens that produce systemic infections in humans. specific examples are provided whenever they might clarify special issues. systemic fungal infections usually are divided into two broad categories: endemic systemic fungal diseases, which occur classically in healthy hosts, and opportunistic fungal diseases, which occur almost exclusively in patients with impaired host defenses. both the increasing frequency of disseminated histoplasmosis and coc ... | 1992 | 1477243 |
| laboratory-acquired sporotrichosis. | a case of laboratory-acquired sporotrichosis is described that was associated with research activities involving isolates of sporothrix schenckii from the 1988 sporotrichosis epidemic in the usa. infection occurred in the absence of apparent trauma or other predisposing factors. the possibility that s. schenckii can invade healthy and intact skin is suggested. | 1992 | 1588467 |
| isolation and characterization of sporothrix schenckii from clinical and environmental sources associated with the largest u.s. epidemic of sporotrichosis. | the largest recorded epidemic of sporotrichosis in the united states occurred in 1988 and involved a total of 84 cases in 15 states. all cases were associated with wisconsin-grown sphagnum moss. twenty-one clinical isolates of sporothrix schenckii and 69 environmental isolates of sporothrix spp. from the epidemic were characterized and compared. the environmental isolates were recovered from 102 samples of sphagnum moss and other material by using direct plating techniques. characteristics exami ... | 1991 | 1864926 |
| detection of cellular immunity with the soluble antigen of the fungus sporothrix schenckii in the systemic form of the disease. | sporothrix schenckii is the etiologic agent of sporotrichosis, a mycosis of world-wide distribution more commonly occurring in tropical regions. the immunological mechanisms involved in the prevention and control of sporotrichosis are not fully understood but apparently include both the humoral and cellular responses. in the present investigation, cellular immunity was evaluated by in vivo and in vitro tests in mice infected with yeast-like forms of s. schenckii. the disease developed systemical ... | 1992 | 1640975 |
| [in vitro antifungal activity of amorolfine, a new morpholine antimycotic agent]. | in vitro antifungal activities of amorolfine (mt-861), a newly developed morpholine antimycotic agent, against a wide range of pathogenic fungal strains were investigated using an agar-dilution method with an imidazole antifungal agent, clotrimazole (ctz), as the reference drug. the results showed that mt-861 had a broad antifungal spectrum, and was active against all of the pathogenic fungi tested except nonpigmented filamentous fungi such as aspergilli and penicillia. dermatophytes and malasse ... | 1991 | 1960861 |
| sporotrichoid infection. two cases. | the authors describe two cases of sporotrichoid infection characterized by the onset, above previous skin accidental injuries, of a papulo-pustular lesion, with further development of centripetal satellite lesions similar to the first one. a precise etiological definition was possible only after the cultural,l mycological and bacteriological examination of biopsy material. the very similar clinical features of the two cases were due to different etiological pathogens: sporothrix schenckii in one ... | 1990 | 1963172 |
| phylogeny and molecular epidemiology of sporothrix schenckii in japan. | mitochondrial dna(mtdna) diversity was investigated in 257 clinical isolants of sporothrix schenckii obtained from 4 districts in japan. s. schenckii was classified into 10 types based on hae iii restriction profiles. phylogeny of types constructed by the method of fitch and margoliash [1] on the estimated sequence divergence within mtdna using the methods of nei and li [2], showed that s. schenckii are grouped into 2 clusters, one group consisting of types 1, 2 and 3, and the other group consis ... | 1991 | 1766461 |
| sporotrichosis presenting as pyoderma gangrenosum. | a 56-year-old female with an eight-year history of corticosteroid therapy for rheumatoid arthritis presented with large, deep, painful ulcers on the left buttock and thigh. the lesions appeared typical of pyoderma gangrenosum. nine separate cultures of the exudate grew sporothrix schenckii. during the course of iodide therapy, the patient expired due to escherichia coli pneumonia. this is the third case report of sporotrichosis presenting as pyoderma gangrenosum and the first report from china. ... | 1991 | 1795732 |
| concurrent infection with sporotrichosis and blastomycosis: an unusual case. | two facial nodules and a pulmonary infiltrate occurred in a twenty-one-year-old man. tissue cultures from one of the facial lesions showed both sporothrix schenckii and blastomyces dermatitidis. cultures from lung biopsy tissue grew b. dermatitidis. this is the first known reported case of sporotrichosis and blastomycosis occurring concurrently in the same cutaneous lesion. | 1991 | 1935247 |
| in vitro comparison of the antifungal activities of r34,000, miconazole and amphotericin b. | in vitro susceptibilities of 78 isolates of pathogenic filamentous fungi to the imidazole compounds r34,000 and miconazole and to amphotericin b were determined using an agar dilution technique. allescheria boydii, sporothrix schenckii and the dematiaceous fungi (cladosporium, fonsecaea and phialophora spp.) were most susceptible to miconazole with minimum inhibitory concentration (mic) values ranging from less than or equal to 0.25 to 32 microgram/ml and with geometric mean mic (g-mic) values o ... | 1978 | 699677 |
| [experimental behavior of sporothrix schenckii and leishmania mexicana in hamsters]. | the macroscopic description of the pathogenic process of sporothrix schenckii and leishmania mexicana spp in hamsters inoculated subcutaneously in the nose provided bases for the differentiation of the behavior of these two microorganisms in a model frequently utilized for their study. sequential observations over 150 days demonstrated that infections caused by these pathogens results initially in edema and erythema followed by loss of hair, necrosis and ulceration. the pus production was a char ... | 1990 | 2135471 |
| new antifungal agents for the systemic mycoses. | the azoles are the prominent broad spectrum oral antifungal agents in use or under clinical investigation for the systemic mycoses. this class of antifungal agents is represented by the marketed drug ketoconazole (nizoral) and the experimental triazoles furthest along in clinical trials in the united states, itraconazole and fluconazole. ketoconazole use is limited by its side effect profile and activity spectrum. itraconazole appears to be better tolerated and less toxic to liver function, does ... | 1990 | 2157984 |
| [detection of amastigotes in cutaneous and mucocutaneous leishmaniasis using the immunoperoxidase method, using polyclonal antibody: sensibility and specificity compared with conventional methods of diagnosis]. | the indirect immunoperoxidase method was evaluated in 265 biopsies with the purpose of increasing the sensitivity of the diagnostic histopathology of tegumentary lesions caused by subspecies of the leishmania braziliensis complex. a diagnosis of leishmaniasis was established by parasitological methods (181) or clinical criteria (12) in 193 patients (72.8%). in the latter group of confirmed cases standard histochemistry and immunoperoxidase were compared with direct examination of tissue scraping ... | 1989 | 2181244 |
| cryptococcus neoformans, candida albicans, and other fungi bind specifically to the glycosphingolipid lactosylceramide (gal beta 1-4glc beta 1-1cer), a possible adhesion receptor for yeasts. | the role of glycosphingolipids as adhesion receptors for yeasts was examined. cryptococcus neoformans, candida albicans, and saccharomyces cerevisiae, as well as histoplasma capsulatum and sporotrichum schenckii (in their yeast phases), bound specifically to lactosylceramide (gal beta 1-4glc beta 1-1cer), as measured by overlaying glycosphingolipid chromatograms with 125i-labeled organisms. an unsubstituted galactosyl residue was required for binding, because the yeasts did not bind to glucosylc ... | 1990 | 2194958 |
| spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi. | homogeneous inoculum suspensions of 29 isolates of clinically important filamentous fungi were adjusted with a spectrophotometer (530 nm) to obtain standardized preparations containing 1 x 10(6) to 5 x 10(6) cfu/ml. colony counts (cfu per milliliter) of 1 x 10(6) to 5 x 10(6) were achieved on three different days for isolates of aspergillus spp., pseudallescheria boydii, and sporothrix schenckii (80% +/- 2% transmission), and colony counts of 7 x 10(5) to 2.9 x 10(6) (70% +/- 2% transmission) we ... | 1991 | 2007647 |
| a prospective necropsy study of arthritis in acquired immunodeficiency syndrome. | thirty-two knee and 23 sternoclavicular joints from 35 subjects with acquired immunodeficiency syndrome were examined prospectively at necropsy. there were two instances of opportunistic infectious arthritis: one caused by staphylococcus aureus, the other by sporothrix schenckii. in five other subjects, para-articular bone was infiltrated by granulomatous or neoplastic sequelae of the human immunodeficiency virus infection. there was no immunohistochemical (p24 antigen) or other evidence for exi ... | 1990 | 2222144 |
| cutaneous sporotrichosis in thailand: first reported case. | a case of cutaneous sporotrichosis is reported for the first time in thailand. the infection occurred in a 33-year-old thai female who has been in good health and had no history of previous trauma or contact with any animals. histopathology revealed pseudoepitheliomatous hyperplasia of the epidermis and a combination of granulomatous and pyogenic reactions in the dermis and subcutaneous tissue. typical asteroid bodies (splendore-hoeppli phenomenon) with central yeast cells were seen. sporothrix ... | 1990 | 2103588 |
| [morphology of spores of sporotrichum schenckii in tissue and in culture]. | | 1975 | 1227389 |
| sporothrix schenckii--a freeze-fracture study. | freeze-fracture electron microscopy of a pathogenic dimorphic fungus sporothrix schenckii revealed planar views of cell structures corresponding to those described already on thin sections. in addition to the characteristic differences in cell wall thickness between conidia, yeast forms and filaments, variations in plasma membrane invaginations were found. in conidia the invaginations were short and abundant, while in yeast forms they were scarce and longer. the plasma membrane of the filaments ... | 1990 | 2213535 |
| a case of sporotrichosis caused by two genetically different sporothrix schenckii strains. | two sporothrix schenckii strains of different mitochondrial dna restriction profiles were isolated from different cutaneous lesions in a 53 year-old woman with sporotrichosis. these results suggest that sporotrichosis can be simultaneously caused by two or more genetically different s. schenckii strains. | 1990 | 2250689 |
| sporotrichosis in nepal. | the first case of sporotrichosis from nepal is reported in a 25-year-old man from a village about 60 km east of kathmandu. he never travelled outside of nepal before and had acquired the lymphocutaneous form of the disease after an accidental injury to the right foot while cutting wood. the diagnosis of the case was made by culturing sporothrix schenckii from the lesions, proving the dimorphic character of the fungus in vitro, its pathogenicity in mice, and its serology. oral potassium iodide th ... | 1990 | 2269567 |
| treatment of systemic sporotrichosis with ketoconazole. | infections of deep soft tissues with the dimorphic fungus sporothrix schenckii are uncommon in humans, and therapy has often required toxic drugs. we report our experience in treating 11 patients who had deep-seated sporotrichosis with ketoconazole, a well-tolerated, orally absorbed antifungal agent. eight infections involved one or more joints, and three involved thoracic, cervical, and widespread cutaneous sites, respectively. for eight patients all evidence of infection resolved during therap ... | 1991 | 2017630 |
| preparation of murine monoclonal antibodies against the yeast phase of the dimorphic fungus sporothrix schenckii. | three murine monoclonal antibodies (mabs) were raised against a cytoplasmic antigen of the yeast phase of the pathogenic fungus sporothrix schenckii using a modification of standard hybridoma technology incorporating the immunosuppressive drug cyclophosphamide. when tested for species-specificity within the pathogenic dimorphic fungi one of these mabs (s5) showed little cross-reactivity by enzyme-linked immunosorbent assay and western blot, though there was some recognition of paracoccidioides b ... | 1990 | 2278087 |
| calcium uptake and efflux during the yeast to mycelium transition in sporothrix schenckii. | a study was made of calcium metabolism during germ tube formation in sporothrix schenckii yeast cells. a net efflux of calcium was observed very early in the transformation process and remained constant thereafter. the efflux of calcium in yeast cells induced to form germ tubes was twice that observed in yeast cells not induced to form germ tubes. two peaks of calcium uptake were observed in germ tube forming yeast cells at 30 and 300 minutes following inoculation, while non-induced yeast cells, ... | 1990 | 2250687 |
| unusual fatty acid in sporothrix schenckii. | sporothrix schenckii was isolated from a specimen from a 75-year-old woman with lymphatic sporotrichosis on the left forearm. a mass of fungi was cultured in liquid sabouraud dextrose broth for three months. crude lipids were extracted from the cultured fungal mass. total fatty acids and free fatty acids were isolated, purified, and then analyzed using thin-layer chromatography, gas liquid chromatography, and mass spectrometry. total fatty acid composition consisted of seven molecular species of ... | 1990 | 2273168 |
| comments on the differentiation of a gliomastix isolated from sputum versus sporothrix schenckii. | | 1975 | 1117891 |
| ultrastructural localization of specific surface antigens in the dimorphic fungus sporothrix schenckii. | the ultrastructural localization of antigens recognized by an antiserum raised against formaline-killed sporothrix schenckii yeast cells was investigated on yeast and mycelial phases of the fungus. immunogold procedures revealed that these antigens were located on the cell surface of both growth phases. labelling was heterogeneous and involved areas of concanavalin a-binding sugar residues. | 1990 | 2362232 |