| development and laboratory evaluation of a new recombinant elisa for the serodiagnosis of lyme disease. | the use of recombinant proteins for serologic testing represents a modern approach for the improved laboratory diagnosis of lyme disease (ld). the aim of the present study was to develop and evaluate a new recombinant elisa (re) for the detection of specific igg and igm antibodies against borrelia burgdorferi. | 2002 | 12422605 |
| prevalence of borrelia burgdorferi species and identification of borrelia valaisiana in questing ixodes ricinus in the lyon region of france as determined by polymerase chain reaction-restriction fragment length polymorphism. | many cases of lyme borreliosis have been reported over the years in the region of lyon, france. the identification and prevalence of borrelia burgdorferi sensu lato in ixodes ricinus were investigated by polymerase chain reaction (pcr) of the flagellin gene and restriction fragment length polymorphism (rflp) analysis. questing ixodes ricinus larvae, nymphs and adults were collected by the flagging method from deciduous forests in four areas in the lyon region of france between october 1994 and s ... | 2003 | 12649714 |
| characteristics of erythema migrans in borrelia afzelii and borrelia garinii infections. | erythema migrans is the most common manifestation of stage i lyme borreliosis. this study investigated the relation between the subspecies of borrelia burgdorferi involved, the time of development and the appearance of the manifestation. the study comprised 33 cases of erythema migrans yielding b. burgdorferi sensu lato by culture of skin biopsy. the species was determined by polymerase chain reaction analysis of the cultivated spirochaetes. in 22 of the cases, b. afzelii was demonstrated, where ... | 2003 | 12685881 |
| diversity within borrelia burgdorferi sensu lato genospecies in switzerland by reca gene sequence. | a total of 874 ixodes ricinus ticks were collected in switzerland to investigate the genetic diversity of the borrelia population. we integrated to the rt-pcr method the dna sequence analysis of a 162-bp fragment of the reca gene. five genospecies were detected: borrelia afzelii, borrelia burgdorferi s.s., borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. a heterogeneous distribution was observed within the b. burgdorferi s.l. genospecies. the most prevalent and diverse genospecies ... | 2004 | 15336411 |
| prevalence of granulocytic ehrlichia and borrelia burgdorferi sensu lato in ixodes ricinus ticks collected from southwestern finland and from vormsi island in estonia. | altogether, 343 adult and 111 nymphal ixodes ricinus ticks collected from parks in turku and suburban and rural islands of the turku archipelago, finland, and 100 adult i. ricinus ticks collected from vormsi island, estonia, were included in this study. using the polymerase chain reaction the ticks were examined for 16s rdna of the ehrlichia phagocytophila genogroup and for borrelia burgdorferi sensu lato reca and flagellin genes. none of the finnish ticks was found to be infected with e. phagoc ... | 2003 | 12716393 |
| recombinant or peptide antigens in the serology of lyme arthritis in children. | the performance of elisas with the recombinant antigens decorin-binding protein a (dbpa), dbpb, and bbk32 (from borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto) and vlse peptide antigen invariable region 6 (ir(6)) were evaluated in the serodiagnosis and follow-up of children with lyme arthritis (la). serum samples were obtained from 52 children with clinically typical and serologically confirmed la. in igg elisas, at diagnosis, 50 samples were positive for bbk32, 51 for dbpa, 40 f ... | 2003 | 12792865 |
| diversity of ixodes-borne borrelia species--clinical, pathogenetic, and diagnostic implications and impact on vaccine development. | among borrelia spirochetes carried by hard ticks belonging to the various ixodes species, at least 10 species can be distinguished. of these, borrelia burgdorferi sensu stricto is involved in human lyme borreliosis in north america and europe, and borrelia garinii and borrelia afzelii in human disease in europe and asia. the pathogenetic significance of the other species is uncertain. although some of the borrelia species are restricted to certain tick species, ixodes ricinus, the vector of lyme ... | 2002 | 12804166 |
| [psoriasis as koebner phenomenon in erythma migrans]. | the koebner phenomenon, described by koebner in 1876, represents the appearance of isomorphic skin lesions in uninvolved skin in patients suffering from certain skin diseases. it can be induced by different mechanical, chemical, thermal and infectious stimuli. a 48 year-old woman developed an exacerbation of a latent psoriasis as a koebner phenomenon in a migrating erythema caused by borrelia afzelii infection. | 2002 | 12444521 |
| characterization of borrelia afzelii isolated from ixodes nipponensis and apodemus agrarius in chungju, korea, by pcr-rflp analyses of ospc gene and rrf (5s)-rrl (23s) intergenic spacer. | nine borrelia afzelii strains, which had been isolated from two vectors, ixodes nipponensis and apodemus agrarius in chungju, korea, were characterized by pcr-rflp analyses of ospc genes and rrf (5s)-rrl (23s) intergenic spacer. drai restriction patterns of chungju strains were identical to those of b. afzelii vs461. but msei restriction patterns of rrf (5s)-rrl (23s) intergenic spacer genes of kk2, km4, kk5 differed from those of previously reported b. burgdorferi sensu lato strains. nine chung ... | 2002 | 12477246 |
| characterization of borrelia burgdorferi sensu lato isolated in moscow province--a sympatric region for ixodes ricinus and ixodes persulcatus. | the relationships between borrelia species and the vector ticks, ixodes persulcatus and ixodes ricinus were examined in a molecular epidemiological study. we conducted a survey in the moscow region which is a sympatric region for both species of tick. we examined 630 unfed i. ricinus and i. persulcatus, ticks collected from four different regions around moscow within an area of 250 km2. eighty-four ticks were culture positive (13.3%) and the prevalence rate varied in each region from 5.7% to 42. ... | 2005 | 15715174 |
| co-feeding transmission and its contribution to the perpetuation of the lyme disease spirochete borrelia afzelii. | to determine whether direct passage of spirochetes between co-feeding vector ticks contributes to the likelihood that the lyme disease spirochete borrelia afzelii will perpetuate in nature, we compared the effects of time and space on transmission efficiency between simultaneously feeding ticks. the likelihood of co-feeding transmission increases with duration of attachment of the infecting tick. co-feeding transmission becomes less efficient as distance from the infecting tick increases. approx ... | 2002 | 12498658 |
| identification of a new borrelia species among small mammals in areas of northern spain where lyme disease is endemic. | the role of small mammals as reservoir hosts for borrelia burgdorferi was investigated in several areas where lyme disease is endemic in northern spain. a low rate of infestation by ixodes ricinus nymphs was found in the small mammal populations studied that correlated with the near-absence of b. burgdorferi sensu lato in 184 animals tested and with the lack of transmission of b. burgdorferi sensu lato to i. ricinus larvae that fed on them. in contrast, questing ticks collected at the same time ... | 2005 | 15746336 |
| improving the specificity of 16s rdna-based polymerase chain reaction for detecting borrelia burgdorferi sensu lato-causative agents of human lyme disease. | 16s rdna sequences of borrelia burgdorferi sensu lato were aligned with the 16s rdna sequences of borrelia hermsii, borrelia turicatae, and borrelia lonestari in order to identify primers that might be used to more specifically identify agents of human lyme disease in ticks in human skin samples. | 2005 | 15752343 |
| prevalence of borrelia burgdorferi sensu lato genomospecies and of the human granulocytic ehrlichiosis (hge) agent in ixodes ricinus ticks collected in the area of monti lepini, italy. | ticks are obligate hematophagous arthropods that are parasites in every class of vertebrates in most regions of the world. they are also considered to be important vectors for the transmission of human infectious diseases. in the present study we used polymer chain reaction (pcr) amplification analysis to determine the prevalence of borrelia burgdorferi and ehrlichia phagocytophila, the agents of, respectively, lyme borreliosis and human granulocytic ehrlichiosis, among ticks inhabiting the area ... | 2003 | 12797900 |
| identification and functional characterization of complement regulator-acquiring surface protein 1 of the lyme disease spirochetes borrelia afzelii and borrelia garinii. | complement regulator-acquiring surface protein 1 (crasp-1) is the dominant factor-h-like protein 1 (fhl-1)- and factor-h-binding protein of borrelia burgdorferi and is suggested to contribute to persistence of the pathogen. the prototype crasp-1 of b. burgdorferi sensu stricto (crasp-1bb) has been formerly characterized. as shown recently, serum-resistant borrelia afzelii strains express a unique fhl-1 and factor h-binding protein, designated crasp-1ba. here, we describe for the first time the i ... | 2005 | 15784581 |
| detection and molecular typing of borrelia burgdorferi sensu lato in ixodes ricinus ticks and in different patient samples from southwest germany. | the prevalence of different genospecies of borrelia burgdorferi sensu lato in infected ticks could be a determinant for the risk of acquiring lyme borreliosis (lb) and its clinical presentation. a total of 7373 ticks and 2761 samples from lb patients from the same area in southwest germany were analyzed by pcr to assess the frequency of the occurrence of lb-associated genospecies. fifteen percent of the tick samples and 19% of the human samples were found positive for the presence of b. burgdorf ... | 2001 | 12530764 |
| borrelia afzelii evidenced by polymerase chain reaction in a biopsy of nipple lymphocytoma. | | 2003 | 12588408 |
| characterization of the vls antigenic variation loci of the lyme disease spirochaetes borrelia garinii ip90 and borrelia afzelii acai. | the vls locus of borrelia burgdorferi b31 consists of 15 silent cassettes and one expression site (vlse), and the presence of the encoding plasmid lp28-1 correlates with high infectivity. recombination between the expression cassette and silent cassettes occurs in vivo, and this process may enable b. burgdorferi to evade the immune response. to determine the characteristics of the vls loci in other borrelia strains, we have cloned and characterized the vls silent cassette loci of borrelia garini ... | 2003 | 12603744 |
| association of borrelia afzelii with rodents in europe. | borrelia burgdorferi sensu lato (s.l.) is maintained in nature by complex zoonotic transmission cycles, involving a large variety of vertebrates as hosts and hard ticks of the genus ixodes as vectors. recent studies suggest that the genospecies of b. burgdorferi s.l. and sometimes their subtypes are propagated by different spectra of hosts, mainly birds and rodents. in order to test the concept of host-association, we analysed the relationships between borrelia genospecies, rodent hosts and i. r ... | 2003 | 12613759 |
| [genetic variants of borrelia afzelii, a pathogen of the ixodes tick borrelioses]. | borrelia afzelii is one of two most important pathogens of the ixodes tick borrelioses (itb) in russia and neighboring countries. this pathogen circulates in various ecosystems and has a wide range of reservoir hosts and transmitters. the results of studies of genetic heterogeneity of the spirochaetae b. afzelii are considered. a total of 139 primary isolates were studied. the isolates were isolated from three species of ixodes ticks at different stages of development and obtained from the labor ... | 2005 | 16173394 |
| tickborne pathogen detection, western siberia, russia. | ixodes persulcatus (n = 125) and dermacentor reticulatus (n = 84) ticks from western siberia, russia, were tested for infection with borrelia, anaplasma/ehrlichia, bartonella, and babesia spp. by using nested polymerase chain reaction assays with subsequent sequencing. i. persulcatus ticks were infected with borrelia burgdorferi sensu lato (37.6% +/- 4.3% [standard deviation]), anaplasma phagocytophilum (2.4% +/- 1.4%), ehrlichia muris (8.8% +/- 2.5%), and bartonella spp. (37.6% +/- 4.3%). d. re ... | 2005 | 16318722 |
| identification of three clinically relevant borrelia burgdorferi sensu lato genospecies by pcr-restriction fragment length polymorphism analysis of 16s-23s ribosomal dna spacer amplicons. | we report the results of a study of the prevalences of three clinically relevant borrelia burgdorferi sensu lato genospecies (borrelia burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii) in 1,040 questing ixodes ticks from all regions of latvia, where lyme borreliosis is endemic. the prevalences of borrelia in ixodes ricinus and ixodes persulcatus were 22.6 and 27.9%, respectively. molecular typing of b. burgdorferi from infected ticks was performed by restriction fragment length ... | 2004 | 15070987 |
| lysine-dependent multipoint binding of the borrelia burgdorferi virulence factor outer surface protein e to the c terminus of factor h. | serum resistance, an important virulence determinant of borrelia burgdorferi sensu lato strains belonging to the borrelia afzelii and b. burgdorferi sensu stricto genotypes, is related to binding of the complement inhibitor factor h to the spirochete surface protein outer surface protein e (ospe) and its homologues. in this study, we show that the c-terminal short consensus repeats 18-20 of both human and mouse factor h bind to ospe. analogously, factor h-related protein 1, a distinct plasma pro ... | 2004 | 15128807 |
| serological description of estonian patients with lyme disease, a comparison with control sera from endemic and non-endemic areas. | serological tests for lyme disease are mostly not well standardized and cases of misinterpretation of test results by clinicians are rather common. the diagnostic value of serologic tests may also depend on the seroepidemiological situation of the population. the aim of the study was to compare the immunoblot pattern of lyme borreliosis patients and control sera from endemic and non-endemic regions and to identify the most suitable interpretation criteria for our immunoblot test. serum samples o ... | 2004 | 15147004 |
| serological confirmation of borrelia burgdorferi infection in dogs in the czech republic. | from the epidemiological point of view, dogs are very important since they are considered a suitable indicator of the spread of human borreliosis. serum samples obtained from healthy, asymptomatic military dogs from 12 different areas in the czech republic were examined for igg antibodies to borrelia burgdorferi sensu lato (s.l.). the total of 399 serum samples were tested by a whole-cell elisa. specific antibodies to borrelia burgdorferi s.l. were detected in 26 cases (6.5%). in different local ... | 2006 | 16437298 |
| sequence typing reveals extensive strain diversity of the lyme borreliosis agents borrelia burgdorferi in north america and borrelia afzelii in europe. | the genetic polymorphism of borrelia burgdorferi and borrelia afzelii, two species that cause lyme borreliosis, was estimated by sequence typing of four loci: the rrs-rrla intergenic spacer (igs) and the outer-membrane-protein gene p66 on the chromosome, and the outer-membrane-protein genes ospa and ospc on plasmids. the major sources of dna for pcr amplification and sequencing were samples of the b. burgdorferi tick vector ixodes scapularis, collected at a field site in an endemic region of the ... | 2004 | 15184561 |
| an in vitro study of the activity of telithromycin against mobile and cystic forms of borrelia afzelii. | in this study the new ketolide telithromycin was tested in vitro against motile and cystic forms of borrelia afzelii, one of the species of borrelia burgdorferi sensu lato. acridine orange staining, dark field microscopy, and transmission electron microscopy were the techniques used to study the influence of telithromycin on the bacteria. the activity was unexpectedly high, 0.0003 microg/ml < mbc < or = 0.0006 microg/ml for the mobile forms after 7 days of incubation at 34 degrees c. mic was < 0 ... | 2006 | 16501899 |
| [eosinophilic fasciitis associated with borrelia afzelii infection]. | a 58-year-old woman suffered from stiffness, swelling, rubor and muscle pain in the extremities one month after she climbed a mountain in kyushu, an island in southern japan. on admission, mild proximal weakness was present in the extremities. her range of motion in the extremities was limited due to firm skin and subcutaneous stiffness which was similar to scleroderma. she showed peripheral blood eosinophilia and hypergammaglobulinemia as well as a high erythrocyte sedimentation rate. an igm an ... | 2004 | 15287514 |
| molecular characterization of the ospa(161-175) t cell epitope associated with treatment-resistant lyme arthritis: differences among the three pathogenic species of borrelia burgdorferi sensu lato. | treatment-resistant lyme arthritis, which may result from infection-induced autoimmunity, is associated with reactivity to a t cell epitope of outer-surface protein a (ospa(161-175)) of borrelia burgdorferi sensu stricto (bb). this syndrome has been noted primarily in the united states where only bb is present, and rarely in europe where borrelia garinii (bg) and borrelia afzelii (ba) predominate. to gain a better understanding of this epitope, we identified its species-specific polymorphisms, d ... | 2004 | 15501399 |
| prevalence of borrelia burgdorferi sensu lato genospecies in host-seeking ixodes ricinus ticks in selected south bohemian locations (czech republic). | in selected localities of ceské budĕjovice and ceský, krumlov districts, well known by stable high incidence of tick-borne encephalitis (tbe) human cases but with low incidence of lyme borreliosis, monitoring of borrelia burgdorferi sensu lato (s.l.) in ixodes ricinus ticks was performed. research was also aimed at the spread of i. ricinus to mountain areas of this region (national park sumava), as well as at investigating this tick for b. burgdorferi s.l. genospecies and tbe virus infection. al ... | 2004 | 15508414 |
| relationships of a novel lyme disease spirochete, borrelia spielmani sp. nov., with its hosts in central europe. | to determine whether the pathogenic variant of lyme disease spirochetes, isolate a14s, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various central european sites for a related spirochete and determined its host association. a14s-like spirochetes infect numerous questing ticks in the petite camargue alsacienne (pc). they frequently infect dormice, but no mice or voles. garden dormice appear to be better reservoir hosts for a14s-like spirochetes than f ... | 2004 | 15528500 |
| [allele variants of borrelia afzelii found by sequencing of the chromosome gene p66]. | sequencing of gene p66 fragments of 246-337 p.b. was performed in 45 isolates of aorrelia afzelii isolated from different transmitting agents and reservoir hosts within the habitation area of the spirochaeta. at least seven allele variants of the pathogenic agent were found indifferent natural foci of the disease. the extent of similarity between the nucleotide sequences of the isolates of the same allele variant was 99.9-100%; the extent of similarity between different allele variants was 98.9- ... | 2006 | 16756001 |
| first isolation and characterization of borrelia burgdorferi sensu lato strains from ixodes ricinus ticks in turkey. | in order to investigate the presence and prevalence of lyme borreliosis (lyme disease) borrelia species, 312 unfed ticks were collected by flagging at a woodland area in trakya, in the european side of turkey, in may 2002. twelve of 299 ixodes ricinus ticks were infected with borrelia spp., as determined by cultivation in bsk medium (prevalence rate 4.0 %). ten pure cultures were subjected to further characterization by sequencing analysis of the 5s-23s rdna intergenic spacer, 16s rdna and flage ... | 2003 | 12909659 |
| perpetuation of the lyme disease spirochete borrelia lusitaniae by lizards. | to determine whether the lyme disease spirochete borrelia lusitaniae is associated with lizards, we compared the prevalence and genospecies of spirochetes present in rodent- and lizard-associated ticks at a site where this spirochete frequently infects questing ticks. whereas questing nymphal ixodes ricinus ticks were infected mainly by borrelia afzelii, one-half of the infected adult ticks harbored b. lusitaniae at our study site. lyme disease spirochetes were more prevalent in sand lizards (la ... | 2006 | 16820453 |
| [genetic heterogeneity of borrelia afzelii in the natural focus of the middle urals]. | as shown by sequencing the spacer rrf (5s)--rrl (23s) in 72 isolates of b. afzelii (one of the causative agents of ixodes tick borne borrelia infections) and the chromosomal gene coding protein p66 in 22 isolates, that in the natural focus located in the middle urals two different genetic subgroups (vs461 and nt28) of this genospecies simultaneously circulate. these subgroups are represented by 5 gene variants (rrf) 5s--(rrl) 23s and 5 allelic variants in gene p66. the latter, similarly to space ... | 2006 | 16830585 |
| a novel lymphocyte transformation test (ltt-melisa) for lyme borreliosis. | diagnosis of active lyme borreliosis (lb) remains a challenge in clinically ambiguous, serologically indeterminant, and polymerase chain reaction-negative patients. lymphocyte transformation tests (ltts) have been applied to detect specific cellular immune reactivity, but their clinical application has been severely hampered by the poorly defined borrelia antigens and nonstandardized ltt formats used. in this study, we describe the development and clinical relevance of a novel ltt using a valida ... | 2007 | 16876371 |
| evaluation of an internally controlled real-time pcr targeting the ospa gene for detection of borrelia burgdorferi sensu lato dna in cerebrospinal fluid. | this study reports the development and evaluation of an internally controlled real-time pcr targeting the ospa gene for detection of borrelia burgdorferi sensu stricto, borrelia garinii, borrelia afzelii and borrelia valaisiana. dna was extracted using qiaamp dna blood mini kit columns. dna from 33 b. burgdorferi sensu lato strains reacted in the assay, whereas no reactivity was observed with dna from four relapsing fever borrelia spp., 11 unrelated spirochaetes, and 31 unrelated microorganisms. ... | 2006 | 16882295 |
| comparison of findings for patients with borrelia garinii and borrelia afzelii isolated from cerebrospinal fluid. | the most common cause of lyme neuroborreliosis in europe is borrelia garinii, followed by borrelia afzelii. however, no series describing patients with culture-confirmed cases of lyme neuroborreliosis have been published, and no comparison of findings for patients with b. garinii and b. afzelii isolated from cerebrospinal fluid (csf) has been reported. | 2006 | 16912943 |
| dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by lps or infected by borrelia. | to specify the role of individual cytokines in the immune response to pyrogens, isolated and cultivated human peripheral blood mononuclear cells (pbmc) were used for the experiments. different pyrogens (lipopolysaccharide from escherichia coli - lps and live borrelia afzelii) were applied and the time course of changes in concentrations of different cytokines in the medium was followed using the elisa method. it was found that nonstimulated human pbmc proliferate under in vitro conditions and pr ... | 2003 | 14535835 |
| comparison of growth of borrelia afzelii, b. garinii, and b. burgdorferi sensu stricto in mkp and bsk-ii medium. | lyme borreliosis is a tick-borne disease caused by genetically diverse borrelia strains including b. afzelii, b. garinii, and b. burgdorferi sensu stricto (s.s.). the aim of the present study was to assess and compare the growth of one strain per species of b. afzelii, b. garinii, and b. burgdorferi s.s. in modified kelly-pettenkofer (mkp) and barbour-stonner-kelly-ii (bsk-ii) medium, and to check for the presence of the overgrowth after inoculating the media with a mixture of two different borr ... | 2004 | 15595391 |
| improved serodiagnosis of erythema migrans using novel recombinant borrelial bbk32 antigens. | the performances of recombinant borrelial bbk32 proteins as antigens in the serology of erythema migrans (em) were evaluated in an elisa. serum samples were obtained from 75 patients from different geographic areas where three borrelial species, borrelia burgdorferi sensu stricto, borrelia afzelii or borrelia garinii, cause lyme borreliosis. antibodies to variant bbk32 proteins were compared with anti-flagella or with anti-ir(6) peptide antibodies. in igg elisa at presentation of em, 65/75 (87 % ... | 2003 | 12808077 |
| use of cfse staining of borreliae in studies on the interaction between borreliae and human neutrophils. | species of the tick-transmitted spirochete group borrelia burgdorferi sensu lato (b. burgdorferi) cause lyme borreliosis. acute borrelial infection of the skin has unusual characteristics with only a mild local inflammatory response suggesting that the interaction between borreliae and the cells of the first-line defence might differ from that of other bacteria. it has been reported that human neutrophils phagocytose motile borreliae through an unconventional mechanism (tube phagocytosis) which ... | 2006 | 17049082 |
| solitary and multiple erythema migrans in children: comparison of demographic, clinical and laboratory findings. | data on european children with erythema migrans (em) are limited. | 2003 | 14735383 |
| epidemiological characteristics and clinical manifestations of lyme borreliosis in croatia. | the goal was to show the epidemiological characteristics and clinical manifestations of lyme borreliosis in croatia. | 2006 | 17153550 |
| effect of tick saliva on mechanisms of innate immune response against borrelia afzelii. | the effect of salivary gland extract (sge) and saliva from the tick ixodes ricinus (l.) on the interaction of borrelia afzelii spirochetes with mouse macrophages as well as on the borreli-acidal effect of calf serum was studied. sge reduced both the number of phagocytosing cells and phagocytosed bacteria. an inhibitory effect of sge on the killing of spirochetes by the alternative pathway of complement activation also was observed. both sge and saliva down-regulated production of proinflammatory ... | 2006 | 17162955 |
| the use of local isolates in western blots improves serological diagnosis of lyme disease in scotland. | nine scottish borrelia burgdorferi isolates were investigated in igg western blot tests. sera previously found to be positive and negative when tested by routine western blots prepared from reference strain b. burgdorferi sensu stricto antigen had different outcomes with these isolates. two isolates, e5 (borrelia afzelii) and g4 (b. burgdorferi sensu stricto) performed well, reproducing western blot-positive results in 90 and 95% of tests, respectively. when antigens from both isolates were inco ... | 2007 | 17172516 |
| [comparative analysis of use of two strains of various genotypes of borrelia burgdorferi sensu lato as antigens for antibody identification in ixodes tick borreliosis by indirect immunofluorescence]. | the results of the indirect reaction of immune-fluorescence (irif) was studied in testing 49 sera of 19 patients with lyme-borreliosis with antigens of genotypes borrelia afzeli (strain jp-21) and borrelia burgdorferi sensu stricto (strain no. 17); rabbit fluorescini-sothiocyanate-marked conjugates to human immunoglobulins m and g as well as polyvalent conjugate were used of. no reliable differences were found between all positive and all negative results. the biggest portion of positive results ... | 2003 | 12822309 |
| dynamics of cytokine production in human peripheral blood mononuclear cells stimulated by lps or infected by borrelia. | to specify the role of individual cytokines in the immune response to pyrogens, isolated and cultivated human peripheral blood mononuclear cells (pbmc) were used for the experiments. different pyrogens (lipopolysaccharide from escherichia coli - lps and live borrelia afzelii) were applied and the time course of changes in concentrations of different cytokines in the medium was followed using the elisa method. it was found that nonstimulated human pbmc proliferate under in vitro conditions and pr ... | 2003 | 14964289 |
| ticks (ixodidae) from passerine birds in the carpathian region. | birds have been found to be a reservoir host of borrelia. in order to assess the situation in slovakia ticks were collected from a total of 3057 mist-netted, ringed and released passerine birds in two locations at 500 m (in 2001) and 1000 m (in 2003) above sea level in the bukovské vrchy hills, part of the carpathian region in the north-east of slovakia. a total of 75 birds of 16 species were infested with subadult ticks of ixodes ricinus species (prevalence of parasitization 5%). sixty-two larv ... | 2006 | 17186172 |
| pathogens and host dna in ixodes ricinus nymphal ticks from a german forest. | unfed nymphs of ixodes ricinus were collected from vegetation in a forest on the outskirts of berlin, germany and were analyzed for host and pathogen dna. pathogens were detected in 47% of the ticks. borrelia afzelii was the commonest pathogen detected, followed by rickettsia helvetica. other pathogens included b. valaisiana, b. garinii, b. burgdorferi sensu stricto, anaplasma phagocytophilum, and a relapsing fever-like borrelia. most of the host dna detected was of rodent origin and was associa ... | 2006 | 17187573 |
| terrestrial distribution of the lyme borreliosis agent borrelia burgdorferi sensu lato in east asia. | lyme borreliosis is the most prevalent tick-borne zoonosis and an important emerging infection in europe, north america, and far eastern countries. the geographical distribution of borrelia spp. and the relationship between borrelia spp. and tick spp. in east asian countries have been studied. in northern asian countries, ixodes persulcatus carries eurasian-type borrelia garinii (20047 type), borrelia afzelii, and asian-type b. garinii (variant nt29), whereas borrelia burgdorferi sensu stricto h ... | 2004 | 15623946 |
| effect of tick salivary gland extract on the cytokine production by mouse epidermal cells. | previous studies have demonstrated that both tick saliva and borrelia burgdorferi sensu lato antigens modulate the cytokine response of the host. in this paper, the effect of salivary gland extract (sge) from ixodes ricinus (l., 1758) ticks on cytokine production by primary cultures of mouse epidermal cells stimulated with borrelia afzelii canica, nato, du merle, mazie, baranton et postic, 1993 spirochetes was analysed. epidermal cells were derived from c3h/hen mice, susceptible to lyme disease, ... | 2004 | 15729950 |
| complementation of a borrelia afzelii ospc mutant highlights the crucial role of ospc for dissemination of borrelia afzelii in ixodes ricinus. | alteration of the outer surface protein (osp) composition--especially that of ospa and ospc--seems to be important for the adaptation of borrelia burgdorferi sensu lato to its endothermic hosts (mammals) and poikilothermic vectors (ticks). ospa possibly mediates adherence to tick midgut cells thus enabling the borreliae to survive in the vector, while ospc is associated with borrelial invasion of the tick salivary glands and infection of the mammalian hosts. here we describe the first successful ... | 2007 | 17267282 |
| [detection of dna of borrelia circulating in novosibirsk region]. | detection of dna of borrelia burgdorferi sensu lato was performed by pcr in taiga ticks ixodes persulcatus, in blood samples and skin bioptates of small forest mammals, and in blood and urine samples of humans after attaching of ticks events. in novosibirsk region both in natural reservoir and in patients with ixodes ticks-borne borreliosis dna of borrelia garinii and borrelia afzelii are detected. dna of these borrelia were detected in 8 from 72 of taiga ticks, in 36 from 298 of blood and skin ... | 2006 | 17297875 |
| comparison of erythema migrans caused by borrelia afzelii and borrelia garinii. | we compared epidemiological and clinical characteristics of patients with erythema migrans (em) caused by borrelia afzelii and borrelia garinii. | 2004 | 15007737 |
| diversity of borrelia burgdorferi sensu lato in russian far east. | thirty strains of borrelia burgdorferi sensu lato have been isolated from ixodes persulcatus ticks and from skin lesions of lyme disease patients in the russian far east from 1997 to 2003. we amplified full-length outer surface protein a (ospa) gene of all strains. blast search and following phylogenetic analysis showed that strains form four well-defined groups. four strains belong to borrelia afzelii species. other strains distributed into tree major groups, identified as borrelia garinii. ind ... | 2005 | 15781992 |
| in vitro susceptibility testing of borrelia burgdorferi sensu lato isolates cultured from patients with erythema migrans before and after antimicrobial chemotherapy. | clinical treatment failures have been reported to occur in early lyme borreliosis (lb) for many suitable antimicrobial agents. investigations of possible resistance mechanisms of the borrelia burgdorferi complex must analyze clinical isolates obtained from lb patients, despite their receiving antibiotic treatment. here, borrelial isolates obtained from five patients with erythema migrans (em) before the start of antibiotic therapy and again after the conclusion of treatment were investigated. th ... | 2005 | 15793100 |
| tick-borne zoonotic bacteria in wild and domestic small mammals in northern spain. | the prevalence and diversity of tick-borne zoonotic bacteria (borrelia spp., anaplasma phagocytophilum, coxiella burnetii, and spotted fever group rickettsiae) infecting 253 small mammals captured in the basque country (spain) were assessed using pcr and reverse line blot hybridization. trapping sites were selected around sheep farms (study 1, 2000 to 2002) and recreational parks (study 2, 2003 to 2005). the majority of the studied mammals (162) were wood mice (apodemus sylvaticus), but six othe ... | 2007 | 17693556 |
| [concentration of sfas and sfasl in the supernatant of pbmc culture from the patients with late lyme borreliosis]. | persistent, inadequate inflammatory response present in late lyme borreliosis may be driven by activated t lymphocytes. we estimated synthesis of extracellular proteins: soluble fas receptor (sfas) and its ligand (sfasl), which might protect t lymphocytes from physiologic apoptosis, in the peripheral blood mononuclear cell (pbmc) culture from patients with late borreliosis. | 2007 | 17702439 |
| susceptibility of borrelia afzelii strains to antimicrobial agents. | the aim of the present study was to determine the susceptibility of borrelia afzelii strains to antibiotics, and to test the hypothesis that persistence of borrelia in skin, after therapy, is a consequence of resistance to the antibiotic used for treatment. ten b. afzelii strains isolated from skin of seven adult patients (two with acrodermatitis chronica atrophicans, five with erythema migrans) were studied. in three patients b. afzelii was isolated from erythema migrans lesion before antibioti ... | 2005 | 15871918 |
| occurrence of borrelia afzelii and borrelia garinii in ixodes ricinus ticks from southern moravia, czech republic. | during the years 1996-2000, a total of 2398 ixodes ricinus ticks were collected in three areas of southern moravia and eastern bohemia, czech republic, and examined by dark-field microscopy for the presence of spirochetes. the prevalence of b. burgdorferi sensu lato in ixodes ricinus ticks varied and depended upon the year. in 1996, the prevalence 6.8% was observed, during 1997 and 1998, it increased to 8.4% and 12.3%, respectively. the lowest prevalence was observed in 1999 (3.6%), and in 2000 ... | 2004 | 15018772 |
| higher mrna levels of chemokines and cytokines associated with macrophage activation in erythema migrans skin lesions in patients from the united states than in patients from austria with lyme borreliosis. | erythema migrans (em) is caused primarily by borrelia afzelii in europe and solely by borrelia burgdorferi in the united states. b. burgdorferi infection in the united states has previously been associated with faster expansion of em lesions and with more associated symptoms, compared with b. afzelii infection in europe. however, reasons for these differences are not yet known. | 2008 | 18171218 |
| concentration of tgf-beta1 in the supernatant of peripheral blood mononuclear cells cultures from patients with early disseminated and chronic lyme borreliosis. | the aberrant inflammatory response is probably involved in the pathogenesis of chronic lyme borreliosis, including chronic lyme arthritis and neuroborreliosis. transforming growth factor-beta 1 (tgf-beta1) is an important anti-inflammatory and immunomodulatory cytokine and its deficient synthesis is linked to exaggerated inflammation and immune response. | 2007 | 18217413 |
| [lyme borreliosis--an overview]. | north american lyme disease and european borreliosis differ in many aspects: whereas in north america infection by borrelia burgdorferi sensu stricto almost invariably leads to erythema migrans, frequently followed by oligoarthritis in untreated individuals, most infections in europe are caused by borrelia garinii and borrelia afzelii and are contracted asymptomatically. a minority of the infected individuals develop neuroborreliosis, consisting of various combinations of meningitis, radiculitis ... | 2005 | 16350535 |
| diversity of ixodes ricinus tick-associated bacterial communities from different forests. | nymphal ixodes ricinus ticks (n=180) were collected from three different areas in the netherlands to investigate the effect of forest composition on tick-associated microbial communities. sampled habitats differed in thickness of leaf litter and humus layers and vegetation associations and were located near amsterdam (beech-oak), ede (birch-oak) and veldhoven (birch-oak). analysis of nine 16s rrna gene clone libraries made from individual ticks showed nearest matches with presumed pathogens cand ... | 2008 | 18355299 |
| lyme borreliosis: insights into tick-/host-borrelia relations. | lyme borreliosis (lb) is a serious infectious disease of humans and some domestic animals in temperate regions of the northern hemisphere. it is caused by certain spirochetes in the borrelia burgdorferi sensu lato (s.l.) species complex. the complex consists of 11 species (genospecies). borrelia burgdorferi sensu stricto (s.s.), borrelia garinii and borrelia afzelii are the major agents of human disease. borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the ixodes ri ... | 2005 | 16405291 |
| borrelia bissettii isolates induce pathology in a murine model of disease. | the spirochete borrelia burgdorferi is a tick-borne pathogen that causes lyme disease. although b. burgdorferi sensu lato is a diverse group of bacteria, only three genospecies, b. burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii, are known to be pathogenic and commonly recognized to cause human disease. to assess the potential of another common genospecies, borrelia bissettii, to induce disease, a mouse model was employed. two colorado isolates of b. bissettii (co-bb) induced l ... | 2008 | 18454594 |
| molecular and serological evidence of borrelia burgdorferi sensu lato in wild rodents in the czech republic. | the aim of the present study was to determine the frequency and spatial distribution of the borrelia species in wild rodents in the czech republic. in total, 293 muscle tissue samples and 106 sera from 293 wild rodents captured in north bohemia and north-east and south moravia were examined for the presence of borrelia spp. and antibodies. muscle samples were investigated with real-time polymerase chain reaction (pcr) with a reca primer set, with dna quantification and melting curve analysis, an ... | 2008 | 18454596 |
| co-inoculation of borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice. | the impact of ixodes ricinus salivary gland extract (sge) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen, b. afzelii, was determined using flow cytometry. sge injected together with spirochetes reduced the numbers of leukocytes and gammadelta-t lymphocytes in infected epidermis at early time-points post infection. in draining lymph nodes, the anti-inflammatory effect of sge was manifested by the decrease of total ... | 2005 | 16475508 |
| improved laboratory diagnostics of lyme neuroborreliosis in children by detection of antibodies to new antigens in cerebrospinal fluid. | laboratory diagnostics in lyme neuroborreliosis need improvement. we hereby investigate 4 new recombinant or peptide borrelia antigens in cerebrospinal fluid in children with neuroborreliosis to evaluate their performance as diagnostic antigens. | 2008 | 18536620 |
| comparison of pcr methods and culture for the detection of borrelia spp. in patients with erythema migrans. | the sensitivities of two pcr assays and culture were compared for the detection of borrelia spp. in skin specimens of 150 patients with typical erythema migrans. in addition, the accuracy of the methods for the identification of borrelia spp. was compared by analysing culture isolates and material obtained directly from skin biopsy specimens. borrelia burgdorferi sensu lato was isolated from 73 (49%) of 150 skin biopsy specimens. using a nested pcr targeting the rrf-rrl region and a pcr targetin ... | 2008 | 18558937 |
| [erythema migrans due to borrelia afzelii]. | | 2006 | 16510103 |
| presence of pathogenic borrelia burgdorferi sensu lato in ticks and rodents in zhejiang, south-east china. | a molecular epidemiological survey was conducted to investigate the presence of pathogenic borrelia burgdorferi sensu lato (s.l.) species in the forest areas of zhejiang province, south-east china. a total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of b. burgdorferi s.l. dna by nested pcr targeting the 5s-23s rrna intergenic spacer. forty-one ticks of four species, haemaphysalis concinna, haemaphysalis longicornis, rhipicephal ... | 2008 | 18628499 |
| prevalence of borrelia burgdorferi sensu lato and anaplasmataceae members in ixodes ricinus ticks in alsace, a focus of lyme borreliosis endemicity in france. | due to the high lyme borreliosis incidence in alsace, in northeastern france, we investigated in 2003-2004 three cantons in this region in order to determine the density of ixodes ricinus ticks infected by borrelia burgdorferi sensu lato and anaplasmataceae. the peak density of nymphs infected by b. burgdorferi sensu lato at munster and guebwiller, where the disease incidence was high, was among the highest reported in europe (105 and 114 per 100 m(2), respectively). in contrast, the peak densit ... | 2006 | 16598024 |
| preparation and purification of recombinant outer surface protein a (rospa) of borrelia burgdorferi sensu stricto and borrelia afzelii. | the recombinant outer surface protein a (rospa) from borrelia burgdorferi is a possible immunogen for protection of infected humans and animals against development of lyme borreliosis (lyme disease), a chronic tick-borne disease characterised by diverse dermatologic, neurologic, rheumatic, and cardiac manifestations. for several years, research and development have been directed towards a vaccine for the prevention of this debilitating disease. numerous animal studies demonstrate that pre-existi ... | 2005 | 16601765 |
| differential role of passerine birds in distribution of borrelia spirochetes, based on data from ticks collected from birds during the postbreeding migration period in central europe. | borrelia spirochetes in bird-feeding ticks were studied in the czech republic. during the postbreeding period (july to september 2005), 1,080 passerine birds infested by 2,240 ixodes ricinus subadult ticks were examined. borrelia garinii was detected in 22.2% of the ticks, borrelia valaisiana was detected in 12.8% of the ticks, borrelia afzelii was detected in 1.6% of the ticks, and borrelia burgdorferi sensu stricto was detected in 0.3% of the ticks. after analysis of infections in which the bl ... | 2009 | 19060160 |
| molecular detection of borrelia bissettii dna in serum samples from patients in the czech republic with suspected borreliosis. | until recently, three spirochete genospecies were considered to be the causative agents of lyme borreliosis (lb) in europe: borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii. however, the dna of borrelia valaisiana, borrelia lusitaniae, borrelia spielmanii and borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of lb. molecular analysis of 12 selected serum samples collected in the regio ... | 2009 | 19187198 |
| borrelia lusitaniae and spotted fever group rickettsiae in ixodes ricinus (acari: ixodidae) in tuscany, central italy. | prevalence of infection by borrelia burgdorferi s.l. and spotted fever group (sfg) rickettsiae was estimated in host-seeking ticks in an area in tuscany, central italy, where lyme borreliosis was reported in a forestry worker. b. burgdorferi s.l. was identified by polymerase chain reaction in 16.7% (95% ci = 10.3, 24.8) of ixodes ricinus (l.) nymphs and 39.6% (95% ci = 26.5, 54.0) of adults. borrelia lusitaniae accounted for 82.9% of positive samples, followed by borrelia garinii (9.8%), borreli ... | 2006 | 16619594 |
| detection of anaplasma phagocytophilum and borrelia burgdorferi sensu lato in dogs in the czech republic. | the aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with anaplasma phagocytophilum or borrelia burgdorferi sensu lato (s. l.) in the czech republic. this data can provide information relevant to human infection. in total, blood samples from 296 dogs and 118 engorged ticks were examined. samples were tested for a. phagocytophilum using polymerase chain reaction (pcr) amplification, nested pcr, and direct sequencing of the 16s rdna, ... | 2009 | 19271998 |
| enhanced culture of borrelia garinii and borrelia afzelii strains on a solid bsk-based medium in anaerobic conditions. | the growth of 29 human strains from the three main pathogenic species of borrelia burgdorferi sensu lato on a solid bsk-based medium was compared in two culture atmospheres: 3% co(2) air and anaerobiosis. all strains grew under anaerobic conditions, whereas only 13 strains were able to grow in aerobiosis with 3% co(2) (p<0.001). in the latter condition, 75% of the b. burgdorferi sensu stricto strains grew versus 33% of the b. garinii and b. afzelii strains. these data suggest that, especially fo ... | 2006 | 16814991 |
| borrelia afzelii gene expression in ixodes ricinus (acari: ixodidae) ticks. | spirochetes belonging to the borrelia burgdorferi sensu lato (s. l.) complex have evolved remarkable ability to survive in diverse ecological niches during transmission cycles between ticks and vertebrate hosts by variable gene expression. to understand the events during spirochete transmission from feeding ticks to hosts, mrna levels of selected b. afzelii genes (bbk32, dbpa, ospa, ospc and vlse) were measured by quantitative real-time sybr green pcr. b. afzelii infected ixodes ricinus nymphs f ... | 2006 | 16989569 |
| detection of the dna of borrelia afzelii, anaplasma phagocytophilum and babesia canis in blood samples from dogs in warsaw. | each month, from march 2003 to february 2004, 34 blood samples from dogs were randomly selected from the blood samples delivered to two veterinary laboratories in warsaw and tested for the dna of borrelia burgdorferi sensu lato, anaplasma phagocytophilum, babesia canis and hepatozoon canis. borrelia dna was detected in seven of the 408 dogs, a phagocytophilum dna was found in two, and b canis dna was found in 48 (11.8 per cent). the dna of h canis was not found in any of the blood samples. seque ... | 2009 | 19363228 |
| detection of borrelia burgdorferi sensu lato in lizards and their ticks from hungary. | to investigate the involvement of lizard species in the natural cycle of borrelia burgdorferi sensu lato (s.l.) in hungary, a total of 186 reptiles belonging to three species--126 green lizards (lacerta viridis), 40 balkan wall lizards (podarcis taurica), and 20 sand lizards (lacerta agilis)--were captured in 2007 and 2008. all ticks removed from the lizards were ixodes ricinus, either larvae (324/472; 68.6%) or nymphs (148/472; 31.4%). more than half (66/126; 52.4%) of l. viridis individuals we ... | 2009 | 19492950 |
| integration of a tick-borne encephalitis virus and borrelia burgdorferi sensu lato into mountain ecosystems, following a shift in the altitudinal limit of distribution of their vector, ixodes ricinus (krkonose mountains, czech republic). | the altitudinal shift in the limit of ixodes ricinus occurrence above the previously established altitude of 750 m above sea level has been monitored over the long-term (2002-2008) in the krkonose mts. (giant mts.), the highest in the czech republic, along two vertical transects in their eastern and central parts (600-1020 and 600-1270 m). ticks were collected by flagging three times annually, and examined individually by pcr or rt-pcr for the presence of borrelia burgdorferi sensu lato or tick- ... | 2010 | 19492952 |
| clinical appearance of erythema migrans caused by borrelia afzelii and borrelia garinii--effect of the patient's sex. | the aim in this survey was to study the clinical characteristics of infections caused by borrelia genospecies in patients with erythema migrans where borrelial origin was confirmed by polymerase chain reaction. the aim was also to study factors influencing the clinical appearance of erythema migrans. | 2006 | 17009065 |
| antigenicity of borrelial protein bbk32 fragments in early lyme borreliosis. | recombinantly produced borrelial bbk32 protein fragments originating from borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii were evaluated as antigens in the serology of lyme borreliosis (lb). in elisa, a mid-portion hydrophilic fragment reacted with lb patient sera. of the 23 patients with culture- or pcr-positive erythema migrans (em), 43 % at diagnosis and 52 % at convalescence were positive for at least one borrelia species-specific variant bbk32 fragment antigen. in ... | 2006 | 17030908 |
| a comparative analysis of molecular markers for the detection and identification of borrelia spirochaetes in ixodes ricinus. | borrelia burgdorferi sensu lato, carried by ixodes ticks, is one of the most significant human pathogens, causing lyme disease. as there is no standardized pcr method for detection and identification of spirochaete dna, we carried out a comparative analysis using a set of complementary primers for three regions in the genomic dna of these bacteria (genes fla and rrs and the non-coding rrs-rrla region). dna extracted from 579 ixodes ricinus ticks was subjected to nested pcr. dna of the examined s ... | 2010 | 20007765 |
| prevalence of borrelia burgdorferi in ixodes ricinus ticks in belgrade area. | lyme borreliosis is vector-borne zoonosis. the causative agent of lyme borreliosis is a spirochete of borrelia burgdorferi (bb) sensu lato complex, which is transmitted by ticks of the ixodes ricinus complex. the aim of our paper is to estimate the prevalence of i. ricinus ticks, the level of their infectivity by bb, and the prevalence of certain genospecies of bb sensu lato in ixodide ticks inhabiting belgrade. | 2010 | 20017716 |
| prevalence of five pathogenic agents in questing ixodes ricinus ticks from western france. | in europe, ixodes ricinus ticks are vectors of many emerging pathogens, including borrelia burgdorferi sensu lato (sl), anaplasma phagocytophilum, spotted fever group rickettsia sp., babesia sp., and very likely bartonella sp. in this study, we looked for the presence of dna of these microorganisms in 572 ticks from two forests in the west of france. dna extraction and polymerase chain reaction (pcr) amplification were performed on individual nymphal, male, and female i. ricinus ticks. amplifica ... | 2010 | 20020814 |
| canine borreliosis: a laboratory diagnostic trial. | the aim of this study was to investigate samples from dogs suggestive of active canine borreliosis (group a) by culture and pcr and the detection of antibodies against borrelia burgdorferi sensu lato in order to confirm a presumptive clinical diagnosis of canine borreliosis by laboratory results. criteria for such a diagnosis were: history of tick exposure, lameness, neurological signs, nephropathy, lethargy, anorexia, and fever. a total of 302 samples comprising edta blood, urine, synovial flui ... | 2007 | 17101241 |
| [the heterogeneity of the ospa gene of borrelia garinii and borrelia afzelii in western siberia and mongolia]. | 48 full-length borrelia garinii and borrelia afzelii from west siberia and mongolia ospa gene nucleotide sequences was determined. four groups of borrelia garinii were revealed using the analysis of nucleotide sequences. the most variable ospa gene region was demonstrated to be included in region where the antigenic determinants of protein were encoded. high homology level was shown for nucleotide sequences corresponding to isolates of borrelia afzelii. | 2009 | 20050161 |
| [enzyme immunoassay-based analysis of ospc recombinant proteins from borrelia garinii and borrelia afzelii isolated in west siberia]. | to study the ability of ospc recombinant proteins from borrelia garinii and borrelia afzelii isolated in west siberia to interact with serum antibodies from patients with tick-borne borreliosis (tbb). | 2010 | 20218339 |
| cutaneous lymphoid hyperplasia presenting as a solitary facial nodule: clinical, histopathological, immunophenotypical, and molecular studies. | to elucidate the clinicopathological, immunophenotypical, and molecular characteristics of cutaneous lymphoid hyperplasia presenting as a solitary facial nodule. | 2006 | 17178981 |
| acrodermatitis chronica atrophicans: a case report and review of the literature. | acrodermatitis chronica atrophicans (aca) is a rare tertiary manifestation of lyme borreliosis, manifesting as inflammatory and atrophic lesions on acral skin. although aca rarely has been reported in the united states, it may be seen in approximately 10% of european cases of lyme borreliosis, most commonly associated with the genospecies borrelia afzelii. we report a presumptive case of aca involving an american woman from pennsylvania with convincing clinical, histopathologic, and serologic fi ... | 2010 | 20540415 |
| seasonal variations in detecting borrelia burgdorferi sensu lato in rodents from north eastern austria. | austria is well known as an endemic area of lyme borreliosis. to assess the annual variation of rodent populations that may host agents of lyme borreliosis we collected rodents in northeastern austria. life traps were set out every six weeks during a year consecutively in one each of the three different zones (hohenau, ernstbrunn, vienna woods) that cover the main habitat characteristics of small mammals in northeastern austria. rodents were collected and identified. samples of heart, urine blad ... | 2006 | 17186171 |
| prevalence of borrelia burgdorferi genospecies in ixodes ricinus ticks from lublin region (eastern poland). | the objective of the study was to determine the prevalence rate of 3 borrelia burgdorferi genospecies in ixodes ricinus ticks collected from wooded areas of the lublin region (eastern poland). a group of 1,813 i. ricinus ticks from 6 districts were examined for the presence of borrelia burgdorferi sensu lato (b.b.s.l.) by polymerase chain reaction (pcr). another group of 438 i. ricinus ticks collected from 4 districts were examined for the presence of b.b.s.l. by culture on bskh liquid medium co ... | 2006 | 17196005 |
| isolation of borrelia afzelii from overwintering culex pipiens biotype molestus mosquitoes. | a total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus culex, aedes and anopheles were collected during the period march 2000-april 2001 from the locality of vysoke myto (eastern bohemia, czech republic). they were examined by dark field microscopy for the presence of spirochetes. the motile spirochetes were observed in 4.2 % of all species of investigated mosquitoes. one spirochetal strain out of the 8 isolation attempts (brz14) was obtained ... | 2006 | 17199258 |
| clinical features and specific immunological response to borrelia afzelii in patients with acrodermatitis chronica atrophicans. | acrodermatitis chronica atrophicans (aca) occurs mostly in europe. borrelia afzelii is considered to be responsible for this manifestation of lyme borreliosis. the aim of the study was to observe the clinical features of the aca and evaluate the specific immunological response to borrelia afzelii. nine patients from an endemic lyme borreliosis region in northeastern poland were studied. the serum samples were tested routinely with ifa and eia and, following testing, with immunoblots using borrel ... | 1999 | 17312680 |
| established and emerging pathogens in ixodes ricinus ticks collected from birds on a conservation island in the baltic sea. | tick-borne pathogens such as lyme borreliosis spirochaetes, anaplasma phagocytophilum, rickettsia spp. and babesia spp. cause a great variety of diseases in animals and humans. although their importance with respect to emerging human diseases is increasing, many issues about their ecology are still unclear. in spring 2007, 191 ixodes ricinus (acari: ixodidae) ticks were collected from 99 birds of 11 species on a bird conservation island in the baltic sea in order to test them for borrelia spp., ... | 2010 | 20868431 |
| effect of gender on clinical and epidemiologic features of lyme borreliosis. | the aim is to highlight the influence of patients' gender on lyme borreliosis and especially erythema migrans (em), focusing on exposure to tick bites, epidemiology, and the clinical picture. all studies were conducted in the county of blekinge, located in southeastern sweden. a prospective study was conducted in 235 individuals (women, n=110; men, n=125) engaged in recreational or occupational activities focusing on exposure to tick bites. a retrospective epidemiologic study evaluating 123,495 ... | 2007 | 17417955 |