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properties of oxaloacetate decarboxylase from veillonella parvula.oxaloacetate decarboxylase was purified to 136-fold from the oral anaerobe veillonella parvula. the purified enzyme was substantially free of contaminating enzymes or proteins. maximum activity of the enzyme was exhibited at ph 7.0 for both carboxylation and decarboxylation. at this ph, the km values for oxaloacetate and mg2+ were at 0.06 and 0.17 mm, respectively, whereas the km values for pyruvate, co2, and mg2+ were 3.3, 1.74, and 1.85 mm, respectively. hyperbolic kinetics were observed with ...19827076619
phenotypic differentiation of some of the veillonella species with the api zym system. 19827116236
effect of ph on growth rates of rumen amylolytic and lactilytic bacteria.the relationship between the ph of the medium and specific growth rates, in well-buffered media at 38.5 degrees c, was determined for three strains of butyrivibrio fibrisolvens and for one strain each of streptococcus bovis, selenomonas ruminantium subsp. lactilytica. megasphaera elsdenii, veillonella alcalescens, and propionibacterium acnes. the ph optima for growth were between 6.1 and 6.6 for all six species, and the upper ph limits were between 7.3 and 7.8. the lower limit ph values for grow ...19827125656
cell wall-associated protein antigens of streptococcus salivarius: purification, properties, and function in adherence.three cell wall-associated protein antigens (antigens b, c, and d) were isolated from mutanolysin-solubilized cell walls of streptococcus salivarius hb and purified to apparent homogeneity by a combination of ion-exchange chromatography, gel filtration, and immunoadsorption chromatography. antigens b and c were also isolated from culture supernatants. antigen b consisted of more than 80% protein and had an apparent molecular weight as determined by sodium dodecyl sulfate-polyacrylamide gel elect ...19827141692
effect of microbial interaction on the colonization rate of actinomyces viscosus or streptococcus mutans in the dental plaque of rats.the resident oral microflora of conventional osborne-mendel rats was challenged with actinomyces viscosus or streptococcus mutans strains. the adherence of the inoculated organism to the tooth surface and the subsequent growth were studied by means of viable counts determination. the initial growth rate of s. mutans in conventional rats was lower than in mono-associated gnotobiotic rats (doubling time, td = 5 h versus td = 1.1 h). the delayed start of growth and the low initial growth rate indic ...19827141698
bacteriology of experimental gingivitis in young adult humans.from replicate trials of experimental gingivitis in four periodontally healthy subjects, 166 bacterial species and subspecies were detected among 3,034 randomly selected isolates from 96 samples. of these bacteria, actinomyces naeslundii (serotype iii and phenotypically similar strains that were unreactive with available antisera), actinomyces odontolyticus (serotype i and phenotypically similar strains that were unreactive with available antisera), fusobacterium nucleatum, lactobacillus species ...19827141708
the influence of microflora and diet on gastro-intestinal parameters. 19827148174
bacteriology of severe periodontitis in young adult humans.a total of 78 bacteriological samples were taken from the supragingival tooth surface after superficial cleaning with toothpicks or from the periodontal sulci of 42 affected sites in 21 adolescents or young adults with severe generalized periodontitis. of 190 bacterial species, subspecies, or serotypes detected among 2,723 isolates, 11 species exceeded 1% of the subgingival flora and were most closely associated with the diseased sulci. eleven others were also sufficiently frequent to be suspect ...19827152665
inhibition of nitrate reduction in some rumen bacteria by tungstate.tungstate prevented the formation of active nitrate reductase in growing rumen bacteria capable of nitrate reduction, but did not directly inhibit the enzyme activity of all strains tested.19807190809
faecal bacterial flora in patients with colon cancer and control subjects.faeces from 10 colon cancer patients and from 10 control subjects were studied by quantitative and qualitative aerobic and anaerobic techniques using a variety of non selective, selective and differential media. in several cases, there were significantly different numbers of organisms of specific types recovered from the two groups studied. most prominent and meaningful findings were the higher frequency of isolation and counts of bacteroides species (especially b. fragilis group) and of c. para ...19817199237
characterization of the adherence properties of streptococcus salivarius.the adherence and aggregation properties of 46 human oral streptococcus salivarius isolates were examined. a total of 41% of the isolates aggregated with whole human saliva, 50% aggregated with human erythrocytes, and 85% adhered to human buccal epithelial cells. strains that aggregated with saliva and erythrocytes usually reacted with streptococcus group k typing serum whereas the non-hemagglutinating strains did not. k+ strains also adhered more strongly to human buccal epithelial cells than k ...19807216420
selective antibacterial properties of lysozyme for oral microorganisms.the antibacterial properties of lysozyme were investigated with oral microorganisms representing the seven serotypes (a through g) of streptococcus mutans, veillonella alcalescens, and the virulent (v) and avirulent (av) strains of actinomyces viscosus t14. growth of bacteria in defined medium was monitored spectrophotometrically after the addition of various amounts (25 mug to 5 mg/ml) of enzyme. no growth inhibition of v. alcalescens was observed. inhibition of a. viscosus t14(v) and a. viscos ...19807216430
ct demonstration of septic thrombosis of the inferior vena cava. 19817217450
bacteria and gallstones. etiological significance.two hundred consecutive gallstone cases have been subjected to bacteriological study employing improved anaerobic culture techniques. in addition to clostridia species, species of anaerobes such as bacteroides fragilis, peptococcus, veillonella, and eubacterium are found to be present, some of which possessed beta-glucuronidase activity. this finding has a certain bearing on the etiology of bile pigment calcium stones. in addition to escherichia coli, bacteroides and clostridium often found in t ...19817238247
identification of a streptococcus salivarius cell wall component mediating coaggregation with veillonella alcalescens v1.cell walls of streptococcus salivarius hb aggregated veillonella alcalescens v1, but cell walls of the mutant s. salivarius hb-v5 did not. we found no correlation between the presence of fimbriae on streptococcal walls and the ability to aggregate veillonella strains. treatment of the walls with lysozyme solubilized a fraction which possessed veillonella-aggregating activity. solubilized cell wall preparations of strain hb contained three major (glyco)proteins as determined by sodium dodecyl sul ...19817251145
aerobic and anaerobic bacteria in tonsils of children with recurrent tonsillitis.tonsils were obtained from 50 children suffering from recurrent tonsillitis. patients' ages ranged from 2.5 to 17 years (mean 6 years); 29 were males and 21 females. the tonsils were sectioned in half after heat searing of the surface and the core material was cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora was obtained in all patients, yielding an average of 7.8 isolates (4.1 anaerobes and 3.7 aerobes) per specimen. there were 207 anaerobes isolated. the pre ...19817271131
role of interbacterial adherence in colonization of the oral cavities of gnotobiotic rats infected with streptococcus mutans and veillonella alcalescens.the role of interbacterial adherence in the colonization of the rate oral cavity was investigated with aggregating and nonaggregating strains of veillonella alcalescens and streptococcus mutans. v. alcalescens v-1 and s. mutans m-7 rapidly formed large stable aggregates when mixed in vitro. aggregates could be reduced in size by sonication, but they could not be completely dispersed, indicating that bonding between the organisms was strong. v. alcalescens v-1 did not coaggregate with s. mutans c ...19817275312
effects of lasalocid or monensin on lactate-producing or -using rumen bacteria.lasalocid or monensin inhibited most of the lactate-producing rumen bacteria (butyrivibrio fibrisolvens, eubacterium cellulosolvens, e. ruminantium, lachnospira multiparus, lactobacillus ruminis, l. vitulinus, ruminococcus albus, r. flavefaciens, streptococcus bovis). minimum inhibitory concentrations ranged from .38 to 3.0 micrograms/ml. among the lactate producers, those that produce succinate as a major end product (bacteroides, selenomonas, succinimonas, succinivibrio) were not inhibited by ...19817275867
enzyme-linked immunosorbent assay for human igg, iga, and igm antibodies to antigens from anaerobic cultures of seven oral bacteria.monocultures of 7 oral bacteria were grown anaerobically, and antigens were partially purified from the supernatant fluids by gel filtration. isoelectric focussing showed that the antigen fractions contained pas-positive material and proteins focussing between ph 3.5 and ph 5.5. specific activity against the 7 antigen fractions was observed for igg, iga, and igm in serum from patients with periodontal disease by means of enzyme-linked immunosorbent assay (elisa). conditions for antigen coating i ...19817288191
comparative in vitro activity of new beta-lactam antibiotics against anaerobic bacteria.several new beta-lactam antimicrobial agents have been introduced in the last few years. in this investigation, the in vitro activities of several recently introduced cephalosporins (cefoperazone, cefotaxime, ceftazidime, and ceftizoxime), moxalactam, and n-formimidoyl thienamycin were compared with those of cefoxitin, clindamycin, and metronidazole against 203 strains of anaerobic bacteria. at achievable serum levels, all of the antimicrobial agents were active against essentially 100% of the s ...19817325628
sucrose-induced ecological response of experimental dental plaques from caries-free and caries-susceptible human volunteers.microbial succession, experimental cariogenicity, and sucrose metabolism were examined in dental plaques which developed on sterile bovine enamel inserts in acrylic palatal appliances. the appliances were worn for a period of 14 days by 10 caries-free and 10 caries-susceptible human volunteers. three of six enamel inserts on each appliance were exposed extraorally to 10% sucrose in 0.85% saline six times a day, and three were exposed simultaneously to 0.85% saline as a control environment. the r ...19817333665
molybdate and sulfide inhibit h2 and increase formate production from glucose by ruminococcus albus.h2 production from glucose by ruminococcus albus was almost completely inhibited by 10(-5) m molybdate only when sulfide was present in the growth medium. inhibition was accompanied by a significant increase in the production of formate. extracts of molybdate-sulfide-grown cells did not contain hydrogenase activity. active enzyme in extracts of uninhibited cells was not inhibited by the molybdate-sulfide-containing growth medium. the results indicate that a complex formed from molybdate and sulf ...19807369826
rapid presumptive identification of anaerobes in blood cultures by gas-liquid chromatography.production of volatile and nonvolatile metabolic acids in blood culture broths by aerobic, facultative anaerobic, and obligate anaerobic bacteria was analyzed by gas-liquid chromatography. anaerobic blood culture isolates were presumptively identified by the qualitative analysis of volatile fatty acids. isolates, with a characteristic gram stain reaction and cellular morphology, were identified by the following acid patterns: bacteriodes fragilis group with acetic and propionic acids; fusobacter ...19807381002
surface vs core-tonsillar aerobic and anaerobic flora in recurrent tonsillitis.specimens from both the surface and the core of tonsils from 23 children with recurrent tonsillitis were cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all patients. the predominant anaerobic isolates were bacteroides sp, fusobacterium nucleatum, anaerobic gram-positive cocci, and eubacterium sp. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, beta-hemolytic streptococci, and haemophilus sp. beta- ...19807411827
complications of sinusitis in children.eight patients (6 to 15 years of age) who had periorbital cellulitis and other complications of sinusitis were studied. both ethmoid and maxillary sinusitis were present in four patients, frontal sinusitis in two, and ethmoid sinusitis and pansinusitis in one patient each. subdural empyema occurred in four patients, in one case accompanied by cerebritis and brain abscess and in another by meningitis. periorbital abscess was present in two children who had ethmoiditis. alveolar abscess in the upp ...19807432842
adherence of streptococcus salivarius hb and hb-7 to oral surfaces and saliva-coated hydroxyapatite.we compared the binding of streptococcus salivarius hb and the mutant hb-7 to oral surfaces in vivo. mutant hb-7 does not aggregate with saliva nor does it bind to buccal epithelium, but it does retain its ability to coaggregate with veillonella and fusobacterium. at 1 h after inoculation into the oral cavity of six volunteers, significantly more s. salivarius hb than hb-7 cells were found adhering to the buccal mucosa (p < 0.05) and to a cleaned tooth surface (p < 0.01); there was no significan ...19807439971
the development of the bacterial flora in normal neonates.the development of the bacterial flora of neonates during the first week of life was studied in 23 babies. specimens of meconium or faeces were collected and swabs taken from the umbilicus and mouth on days, 1, 2, 3 and 6. the bacteria present were isolated on a variety of plain and selective media. the predominant faecal organisms by the end of the first week were anaerobes. bifidobacteria were isolated from all the neonates and bacteroides and clostridia were isolated from 78.3%. bifidobacteri ...19817463467
aerobic and anaerobic bacteriology of adenoids in children: a comparison between patients with chronic adenotonsillitis and adenoid hypertrophy.adenoids were obtained from 18 children with chronic adenotonsillitis (group a) and from 12 others with adenoid hypertrophy (group b). patients' ages ranged from 20 months to 15 years (mean 6 years); 18 were males and 12 females. the adenoids were sectioned in half after heat searing of the surface, and the core material was cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all patients, yielding an average of 7.8 isolates (4.6 anaerobes and ...19817464399
effect of proteolytic enzymes on the lysis and growth of oral bacteria.previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ...19947478762
a comparative study of glucose and galactose uptake in pure cultures of human oral bacteria, salivary sediment and dental plaque.the ability to utilize glucose and the weaker sugar acidogen, galactose, was surveyed in salivary sediment, pooled dental plaque, and in pure cultures of the bacteria that numerically comprise most of the bacteria in these mixed microbial systems. except for a veillonella isolate, which showed no uptake of either sugar, glucose was utilized more rapidly than galactose by the 27 pure cultures tested and by both sediment and plaque. this sugar difference was also seen for two other measures of gly ...19957487576
phylogenetic placement of dialister pneumosintes (formerly bacteroides pneumosintes) within the sporomusa subbranch of the clostridium subphylum of the gram-positive bacteria.the nucleotide sequence of the 16s rrna gene of the type strain of dialister pneumosintes was determined. phylogenetic analysis revealed that this species belongs to the sporomusa branch of the clostridium subphylum of the gram-positive bacteria and should therefore be excluded from the family bacteroidaceae. within this branch, which encompasses several other gram-negative taxa, such as acidaminococcus, pectinatus, phascolarcobacterium, quinella, selenomonas, and zymophilus, dialister showed a ...19957537075
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
comparison of periodontal disease in hiv seropositive subjects and controls (ii). microbiology, immunology and predictors of disease progression.the aims of this study were to compare the prevalence of suspected periodontal pathogens in subgingival plaque from 29 hiv seropositive and 27 control subjects and to determine the association of these bacteria with periodontal destruction. subgingival plaque was collected from the mesiobuccal sites of all teeth, except 3rd molars. bacteria were identified and enumerated using non-isotopic whole chromosomal dna probes and a colony lift method. at baseline, hiv seropositive subjects had significa ...19957560241
expression of the sodium ion pump methylmalonyl-coenzyme a-decarboxylase from veillonella parvula and of mutated enzyme specimens in escherichia coli.the structural genes of the sodium ion pump methylmalonyl-coenzyme a (coa)-decarboxylase from veillonella parvula have recently been cloned on three overlapping plasmids (pjh1, pjh20, and pjh40) and sequenced. to synthesize the complete decarboxylase in escherichia coli, the genes were fused in the correct order (mmdadecb) on a single plasmid (pjh70). a dna region upstream of mmda apparently served as promoter in e. coli because expression of the mmd genes was not dependent on the correct orient ...19957601825
fermentation of radiolabelled substrates by batch cultures of caecal microflora maintained in a continuous-flow culture.glucose- and lactose-based media containing either 14c-labelled glucose, galactose, lactose or lactic acid were inoculated with anaerobic cultures of chicken caecal bacteria maintained for 121 d in a continuous-flow (cf) culture. the culture was previously shown to reduce salmonella colonization in the caeca of chicks inoculated with the culture and the reduction was associated with increases in volatile fatty acids. the distributions of 14c were determined among the fermentation products, espec ...19957615424
[nosocomial infections in present-day traumatological-orthopedic hospitals].nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ...19957626997
treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...19957641626
characterization of inhibitory effects of suspected periodontopathogens on osteogenesis in vitro.by using an in vitro bone-forming culture system, the chick periosteal osteogenesis (cpo) model, the direct effects on osteogenesis of sonicated extracts derived from oral bacteria were examined. both extracts from bacterial species having strong associations with periodontal diseases (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and prevotella intermedia, hereinafter referred to as suspected periodontopathogens) and extracts from species not correlated with periodontal diseas ...19957642257
detection of tet(m) and tet(o) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease.the polymerase chain reaction was used to examine 114 tetracycline-resistant anaerobic and facultative anaerobic bacterial isolates from patients with periodontal disease for the tet(m) and tet(o) genes. a 740-base-pair fragment of the tet(m) gene was amplified from 84 of 114 isolates, and a 519-base-pair fragment of the tet(o) gene was amplified from 13 streptococcal isolates. six of 7 tetracycline-resistant isolates of veillonella spp. and tetracycline-resistant isolates of eubacterium spp. (n ...19957675524
synergism of lactate and succinate as metabolites utilized by veillonella to inhibit the growth of salmonella typhimurium and salmonella enteritidis in vitro.the inhibition of salmonellae growth by a veillonella bacterium isolated from the cecal contents of adult chickens was examined. the veillonella isolate was grown on an agar medium supplemented with 175 mumol of lactate or succinate/ml. either 0, 100, 125, 150, or 175 mumol of succinate/ml was added to the lactate medium; either 0, 100, 125, 150, or 175 mumol of lactate/ml was added to the succinate medium; and the ph of all media was adjusted to 6.0. agar overlays of veillonella cultures grown ...19957677651
relationship of oral microflora with oral health status in parkinson's disease.parkinson's disease (pd) patients report an increased craving for sweets, which may have an effect on microflora. we compared patients of pd who crave sweets with pd patients who do not. age- and sex-matched control subjects were used, with 14 subjects in each group. a plaque sample was taken from tooth #18 with a curette and placed into rtf, homogenized, and plated onto selective and non-selective media. microflora were expressed as % cfu's of total anaerobes. statistical analysis was performed ...19947716701
predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ...19957722848
detection and incidence of the tetracycline resistance determinant tet(m) in the microflora associated with adult periodontitis.subgingival plaque samples were collected from 68 patients with adult periodontitis, enumerated on trypticase-soy blood agar plates, with and without tetracycline at 4 micrograms/ml, and incubated anaerobically for 5 days. each different colony morphotype was enumerated, and a representative colony was subcultured for identification and examined for the tetracycline resistance gene tet(m). both pcr amplification and dna hybridization, using a fragment of tet(m) from tn1545, were used to detect t ...19957730961
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...19947851086
a comparison of the microbial flora in carious dentine of clinically detectable and undetectable occlusal lesions.it is not known whether the aetiology of occlusal hidden caries lesions (hcl) is identical to that of small visible lesions (svl). previous studies of the microflora of hcl suggest that relatively few species can be isolated. the aim of the present study was to compare the bacterial composition of dentine from 10 hcl and 17 svl in a population of children aged 8-18 years. the following bacteria were identified: actinomyces spp., mutans streptococci, streptococcus sanguis, streptococcus oralis, s ...19957867050
head and neck space infections in infants and children.a retrospective study was performed in 117 children with head and neck space infections treated at the children's hospital of pittsburgh from january 1986 through june 1992. peritonsillar space infections were the most common (49%), followed by retropharyngeal (22%), submandibular (14%), buccal (11%), parapharyngeal (2%), and canine (2%) space infections. the most common pathogens isolated (n = 78) were the aerobes beta-hemolytic streptococcus (18%) and staphylococcus aureus (18%), the anaerobes ...19957870436
[therapeutic possibilities in recurrent infantile tonsillitis].the present study compares two therapy protocols in 40 children with recurrent tonsillitis. twenty of them were randomly treated with penicillin, and the remaining 20 received penicillin + metronidazole. clinical and microbiological assessment of the results was made. the association of penicillin and metronidazole was most efficient, probably due to its activity against streptococus pyogenes and anaerobic betalactamase-producing bacterias. the high failure rate in the penicillin group states th ...19947873233
single polymerase chain reaction for the detection of tetracycline-resistant determinants tet k and tet l.oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants tet k and tet l. forty-three isolates representing 11 genera carrying tet k and/or tet l determinants gave appropriate pcr products. the pcr products hybridized with labelled tet k or tet l probes, and differentiated the tet k from the tet l pcr products. we confirmed that two haemophilus aphrophilus carried the tet k determinant and two veillonella parvula carried the tet l determ ...19947877638
anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory concentrations.in the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ...19947924212
the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ...19947936718
in vitro activity of meropenem compared with imipenem, metronidazole, ampicillin, and ampicillin/sulbactam against anaerobes.the aim of the present study was to compare the in vitro activity of meropenem (ici 194660, cas 96036-03-2) with imipenem, metronidazole, clindamycin, ampicillin and ampicillin/sulbactam against a variety of anaerobic bacteria using an agar dilution method. 423 clinical isolates were tested belonging to 70 species of 15 anaerobic genera. they included bacteroides fragilis (n = 62), bacteroides thetaiotaomicron (n = 45), prevotella bivia (n = 11), fusobacterium nucleatum (n = 12), clostridium per ...19947945523
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...19947979307
the predominant cultivable flora of sound and carious human root surfaces.little detailed knowledge exists about the composition of the human root flora. therefore, the predominant cultivable flora (pcf) was determined for samples of: (1) plaque from sound root surfaces (srs) in eight subjects without root caries, (2) plaque from incipient root lesions (irl) in eight subjects with root caries, and (3) carious material from advanced root lesions (arl) in nine other subjects with root caries. generally, one root surface was sampled per subject, and organisms-358, 512, a ...19947983259
chemostat flow cell system: an in vitro model for the evaluation of antiplaque agents.we developed an experimental in vitro model of dental plaque to assess the potential efficacy of antiplaque agents. the model used a chemostat, which provided a continuous source of 5 species of oral bacteria grown in an artificial "saliva-like" medium. this mixture was pumped through six flow cells, each containing two types of surfaces on which plaque formed and was subsequently measured. formation of bacterial plaque on hydroxyapatite surfaces was assessed by measurement of the dna and protei ...19947983262
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...19947995646
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...19948004324
the effects of a 0.3% triclosan-containing dentifrice on the microbial composition of supragingival plaque.144 subjects completed a 6-month, double-blind study which examined the effects of a 0.3% triclosan/2% copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. the subjects were randomly assigned to use, in an oral hygiene program, either the triclosan/copolymer/fluoride test dentifrice or a control dentifrice. the latter had the same formulation as the test dentifrice except it did not contain triclosan. supragingival plaque was collected from the buccal and ...19948034778
anaerobic flora of the conjunctival sac in patients with aids and with anophthalmia compared with normal eyes.relatively few investigations of anaerobic bacteria as ocular flora have been conducted, and their results have been contradictory. the conjunctival sacs of 22 normal subjects and of 14 patients with acquired immunodeficiency syndrome, and 22 anophthalmic sockets were cultured for anaerobic bacteria thirty-four (77.3%) of the 44 eyes of normal subjects harbored anaerobic bacteria; propionibacterium acnes was present in 28 eyes (63.6%), lactobacillus species in 6 eyes (13.6%), and veillonella spe ...19948079632
cytotoxicity versus antibacterial activity of some antiseptics in vitro.through the development of new techniques for bacterial growth and isolation, obligate anaerobes have been shown to be more prevalent than previously thought in the pathogenesis of periapical and pulpal diseases. a follow-up study was conducted to examine the germicidal effect of 0.05% naocl, 10 mg/ml metronidazole and supernatant of ca (oh)2 (0.025 ml) on four anaerobic microorganisms commonly found in root canals, and to compare the toxicity of these substances on cell cultures. in vitro testi ...19938100851
antibacterial effects of various endodontic medicaments on selected anaerobic bacteria.this study was undertaken to determine the antibacterial effects of various endodontic medicaments against six selected anaerobic bacteria. the experiment involved the testing of the vapors of six medicaments using agar plates streaked with the bacteria. a zone of inhibition was recorded for each plate and the results were analyzed statistically. formocresol produced significantly larger zones of inhibition than any of the other medicaments.19938120484
bacterial colonization and degradation of demineralized dentin matrix in situ.lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...19948124693
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...19948151907
fortuitous detection of prosthetic valve endocarditis caused by an uncommon etiologic agent. 19948159046
impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis.the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ...19948195981
sequence of the sodium ion pump methylmalonyl-coa decarboxylase from veillonella parvula.the genes encoding methylmalonyl-coa decarboxylase from veillonella parvula were cloned on plasmids using oligonucleotides derived from n-terminal amino acid sequences as specific probes. the entire dna sequence of the methylmalonyl-coa decarboxylase genes together with upstream and downstream regions was determined. the genes encoding subunits alpha (mmda), delta (mmdd), epsilon (mmde), gamma (mmdc), and beta (mmdb) of the decarboxylase were clustered on the chromosome in the given order. the p ...19938227015
the effect of glass-ionomer cement on carious dentine: an in vivo study.the effect of a glass-ionomer cement sealant on carious dentine was investigated clinically and bacteriologically. the study group consisted of 24 molar teeth, with small clinical visible occlusal dentine lesions, in 13 children (aged 7-18 years). twenty of these molars were filled and sealed with a glass-ionomer cement (fuji ionomer type iii) and 4 with a resin sealant (delton). from each molar, two dentine samples were collected aseptically with a time interval of 7 months. the first sample wa ...19938242680
antibacterial effects of various endodontic irrigants on selected anaerobic bacteria.this study was undertaken to determine the antibacterial effects of various endodontic irrigants against six selected anaerobic bacteria. the organisms were mixed separately with dilutions of each of the irrigants in tubes containing fluid thioglycollate broth and allowed to remain in contact for specific time periods before being evaluated for growth. of the six irrigants tested, chlorhexidine appeared to be the most effective antibacterial substance. hydrogen peroxide, sodium hypochlorite and ...19938243347
microbiology of the pericoronal pouch in mandibular third molar pericoronitis.the microorganisms associated with mandibular third molar pericoronitis were investigated using direct microscopy and anaerobic culture method. the pericoronal pouch was sampled with paper points in a) 8 patients without mandibular third molar pericoronitis and b) 6 patients with mandibular third molar pericoronitis. under the microscope, the microflora was found to be a complex mixture comprising gram-positive and gram-negative cocci, rods and filaments (including fusiform and curved rods), mot ...19938265205
tetracycline resistance in periodontal pathogens.antimicrobial agents are used in combination with debridement to eliminate putative periodontal pathogens, including porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans, from diseased tissues. the most frequently used antimicrobial agents are the tetracyclines. however, these agents are not effective in some patients. this lack of efficacy may be due to antimicrobial resistance. as many as 75% of the bacteria in the subgingival flora may be resistant to tetr ...19938324137
the immediate and long-term effects of invasive and noninvasive pit and fissure sealing techniques on the microflora in occlusal fissures of human teeth.the purpose of this study was to evaluate the effect of acid conditioning and occlusal sealant on microbial colonization of pit and fissures submitted to ameloplasty or left intact. human enamel blocks containing fissures prepared from the occlusal surfaces of unerupted third molars were implanted in occlusal fillings in molars of 12 patients for seven, 30, 60, and 120 days. after seven days of exposure to the oral environment, the pit and fissure blocks were removed and found to be colonized, m ...19938327360
lung abscess: analysis of 66 cases from 1979 to 1991.sixty-six patients with bacterial lung abscess were treated between 1979 and 1991 in our hospital. among these patients, death occurred in one of the 42 cases of community-acquired infection (mortality rate: 2.4%) and in 16 of the 24 cases of nosocomial infection (mortality rate: 66.7%). of all 66 cases, 55 were culture-positive, and the etiologic agents isolated from 24 of the culture-positive cases were found to be anaerobic bacteria. the most common aerobes isolated from the foci were identif ...19938358116
analysis of intestinal flora of a patient with congenital absence of the portal vein.a 14-year-old female patient, admitted for a closer examination of liver tumour (hepatocellular adenoma), was diagnosed as having a congenital absence of the portal vein. the blood ammonia level (approximately 120 micrograms dl-1) in the superior mesenteric vein was markedly low compared to the normal value of 300-350 micrograms dl-1 in the portal vein. the decreased ammonia concentration and urease activity of the patient's faeces were demonstrated. the dominant intestinal flora in the faeces o ...19938364525
the effects of triclosan and zinc citrate, alone and in combination, on a community of oral bacteria grown in vitro.a mixed-culture chemostat system has been used as a more stringent laboratory system for evaluation of the antimicrobial effects of triclosan and zinc citrate. the inhibitors were added alone, and in combination, as a pulse (a high initial inhibitor concentration which decreased with time) or as a dose (concentration of inhibitor increased with time) to give maximum concentrations of 34.5 mumol/l triclosan and 39.8 mumol/l zinc citrate. when dosed, triclosan inhibited a. viscosus and all five gr ...19938380287
comparative evaluation of the rapid id 32a kit system, miniaturized standard procedure and a rapid fermentation procedure for the identification of anaerobic bacteria.the rapid id 32a and a rapid fermentation procedure were compared with a miniaturized standard procedure for the identification of 102 isolates of anaerobic bacteria belonging to 11 genera. the miniaturized standard procedure identified all 102 isolates at the genus level and 90 at the species level. the rapid fermentation procedure failed to identify 12 and 24 isolates at the genus and species levels respectively and is unsatisfactory. the rapid id 32a system misidentified 4 isolates (4%) at th ...19938392248
the effect of tetronasin and monensin on fermentation, microbial numbers and the development of ionophore-resistant bacteria in the rumen.the gram-negative rumen bacteria fibrobacter succinogenes s85, prevotella ruminicola m384 and veillonella parvula l59 were grown in media containing successively increasing concentrations of the ionophores, monensin and tetronasin. all three species became more resistant to the ionophore with which they were grown. increased resistance to one ionophore caused increased resistance to the other, and cross-resistance to another ionophore--lasalocid--and an antibiotic--avoparcin. recovery of tetrona ...19938407673
microbial colonization of human tooth surfaces. 19938494649
a comparative study of cephradine, amoxycillin and phenoxymethylpenicillin in the treatment of acute dentoalveolar infection.a comparative double blind trial was undertaken of the efficacy of cephradine, amoxycillin and phenoxymethylpenicillin in the treatment of acute dentoalveolar abscesses. patients were admitted to the trial with acute dentoalveolar abscesses with systemic involvement and assessments were made of pain, swelling, temperature and lymphadenopathy. after admission to the trial appropriate surgical therapy was undertaken. the patients were then given either phenoxymethylpenicillin 250 mg four times dai ...19938494665
antibacterial activity of oral antibiotics against anaerobic bacteria.the spectrum and the antibacterial susceptibility of anaerobic bacteria to oral antibiotics isolated from clinical specimens was assessed in two different centres, the first receiving specimens from university departments and the second from general practitioners and small hospitals. susceptibility was studied with a microtiter ready-to-use panel system, using the manufacturer's modified wilkins-chalgren's broth as the test medium for the following antibiotics: ampicillin, ampicillin+sulbactam, ...19938508686
dental plaque development on defined streptococcal surfaces.coaggregations between bacterial species have been widely studied in vitro but not in the mouth. a new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. chips were covered with streptococcus oralis atcc 10557, which coaggregated with many oral species, or streptococcus gordonii s7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. there were no di ...19938510979
microbiology of early supragingival plaque development after delmopinol treatment.the aim of this double blind, cross-over, microbiological study was to compare the effect of topical application of the plaque control agent 0.5% delmopinol hcl with placebo on early supragingival plaque formation. six subjects underwent 7 periods (0.5, 1, 2, 8 and 24 h and 3 and 7 days) of placebo and delmopinol application, respectively. at the start of each study period the teeth were professionally cleaned and 2 ml of placebo and delmopinol 0.5%, respectively, were applied on all teeth (twic ...19938510982
investigation of the influences of puberty, genetics, and environment on the composition of subgingival periodontal floras.the classical twin model was utilized in this study in an attempt to determine the importance of host genetics to the composition of the subgingival flora. simultaneously, the effect of puberty on the flora composition was assessed. the compositions of the floras were significantly different at ages 11 and 14 in the same people, indicating that transition to an adult flora composition may be initiated during puberty. however, the numbers of subjects who had prepubertal and postpubertal testoster ...19938514392
isolation and characterization of coaggregation-defective (cog-) mutants of streptococcus gordonii dl1 (challis).streptococcus gordonii dl1 (challis) bears coaggregation-mediating surface adhesins which recognize galactoside-containing surface polysaccharides on streptococcus oralis 34, streptococcus oralis c104, and streptococcus sm pk509. fifty-nine spontaneously-occurring coaggregation-defective (cog-) mutants of s. gordonii dl1 unable to coaggregate with partner streptococci were isolated. six representative cog- mutants were characterized by their coaggregation properties with four actinomyces naeslun ...19958519477
veillonella myositis in an immunocompromised patient.infectious myositis is rather uncommon. when caused by anaerobic organisms, myositis is usually polymicrobial. trauma, ischemia, or a contiguous focus of infection is often an antecedent of myositis. we report a case of monomicrobial veillonella myositis in an immunocompromised patient. the infection responded to debridement and therapy with metronidazole.19958527568
meningitis caused by mixed anaerobic species complicating tethered cord syndrome. 19958527587
adherence, accumulation, and cell division of a natural adherent bacterial population.developing dental bacterial plaques formed in vivo on enamel surfaces were examined in specimens from 18 adult volunteers during the first day of plaque formation. an intraoral model placing enamel pieces onto teeth was used to study bacterial plaque populations developing naturally to various cell densities per square millimeter of surface area of the enamel (w. f. liljemark, c. g. bloomquist, c. l. bandt, b. l. philstrom, j. e. hinrichs, and l. f. wolff, oral microbiol. immunol. 8:5-15, 1993). ...19968576054
comparison of diagnostic tests for bacterial kidney disease in juvenile steelhead trout (oncorhynchus mykiss).in order to accurately diagnose bacterial kidney disease caused by renibacterium salmoninarum in steelhead trout, kidney tissue from experimentally infected fish was evaluated using a commercially available enzyme-linked immunosorbent assay (elisa) test kit, fluorescent antibody (fa) testing, bacteriologic culture, and histopathology. seventy-five steelhead trout were randomly assigned to 1 of 4 groups and intraperitoneally inoculated with 0.15 ml saline (n = 20), 1 x 10(10) organisms/ml (n = 18 ...19958580171
microbiological assessment of an improved stannous fluoride dentifrice.recently, a new stabilized stannous fluoride (snf2) dentifrice (ssf) has been developed. the aim of the present work was to examine the antimicrobial activity of snf2, and to assess the long-term microbial safety of this dentifrice in a series of in vitro and clinical evaluations. results of in vitro time-kill experiments with representative oral bacteria demonstrated that snf2 exerts broad antimicrobial activity against both gram-positives and gram-negatives and, in particular, has potent activ ...19958593200
microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice.a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ...19958602338
incidence of anaerobes in ventilator-associated pneumonia with use of a protected specimen brush.the role of anaerobic bacteria in ventilator-associated pneumonia (vap) has been little investigated. in this study we analyzed the incidence of anaerobes in patients with a first episode of bacteriologically documented vap (> 10(3)cfu/ml), using protected specimen brushes (psb). we particularly took care to preserve anaerobic conditions during transport and the microbiological procedure. two groups were considered: group a with anaerobic bacteria recovered from psb, with or without anaerobes, a ...19968616556
studies into the microbial spectrum of apical periodontitis.this study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. a total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. bacteria were identified by applying the following techniques: culturing on various media, gram-staining and using commercially available biochemical test strips. in addition, prevotella intermedia and porphyromonas endodontalis were d ...19958626206
crystallisation of the flavodoxin from megasphaera eldenii . 19968674647
purification and partial characterisation of the e14k mutant of flavodoxin from megasphaera elsdenii. 19968674684
crystallisation of the flavodoxin from megasphaera eldenii. 19968674697
characterization of xanthine dehydrogenase from the anaerobic bacterium veillonella atypica and identification of a molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor.the molybdenum-containing iron-sulfur flavoprotein xanthine dehydrogenase from the anaerobic bacterium veillonella atypica has been purified approximately 800-fold with a yield of approximately 40% and a specific activity of approximately 70 micromol ferricyanide reduced x min(-1) x mg protein(-1) with xanthine as electron donor, which corresponds to approximately 30 micromol xanthine oxidized x min(-1) x mg protein(-1) with methylene blue as electron acceptor. the 129-kda enzyme was a non-coval ...19968706691
elimination of bacteraemia after dental extraction: comparison of erythromycin and clindamycin for prophylaxis of infective endocarditis.erythromycin and clindamycin are currently recommended for antibiotic prophylaxis of infective endocarditis in predisposed patients allergic to penicillin undergoing oral invasive procedures. thirty-eight healthy patients were randomized to receive either erythromycin (1 g) or clindamycin (0.6 g) orally 1.5 h prior to dental extraction. blood samples for microbiological investigation were collected before, during and 10 min after surgery and were processed by lysis filtration under anaerobic con ...19968722544
anaerobic septic bursitis: case report and review. 19968722967
veillonella infections in children.from 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. eighty-three veillonella spp. were recovered from 83 children (4%). most veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. the infections were polymicrobial in 79 (95%) patients, but in 4 (5%) patients, veillonella species were recovered in pure culture. the predisposing conditions associated with the recovery of these organisms were previous sur ...19968727920
the effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system.in order to develop an improved method to evaluate antimicrobial agents for use in clinical dentistry, a constant-depth film fermenter (cdff) has been used to generate biofilms of fixed depth comprising nine species of bacteria commonly found in dental plaque in health and disease. these bacteria were grown together initially in a conventional chemostat which was used to inoculate the cdff over an 8 h period. medium was then supplied directly to the cdff and biofilms allowed to develop. the biof ...19968760321
inhibition of salmonella typhimurium by the products of tartrate metabolism by a veillonella species.the inhibition of the growth of salmonella typhimurium by a veillonella species grown on media supplemented with tartrate was examined. growth of salmonella typhimurium was not inhibited by the concentrations of products metabolized by veillonella cultures on media supplemented with 0 or 50 mmol l-1 of tartrate, but was inhibited on media supplemented with 100 or 150 mmol l-1 of tartrate. inhibition of salm. typhimurium was correlated with the increased production of acetate and propionate from ...19968760327
effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria.although the mechanism responsible for the clinical antiplaque efficacy of oral antiseptics is generally considered to be primarily one of bactericidal activity, it has been suggested that oral antiseptics may have additional effects on bacteria exposed to sublethal levels. studies reported herein, investigated the effects of sublethal levels of an essential oil-containing antiseptic mouthrinse (listerine antiseptic, warner-lambert co., morris plains, nj) on selected activities of representative ...19968783049
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