| identification of staphylococcus saprophyticus isolated from patients with urinary tract infection using a simple set of biochemical tests correlating with 16s-23s interspace region molecular weight patterns. | the emergence of staphylococcus spp. not only as human pathogens, but also as reservoirs of antibiotic resistance determinants, requires the development of methods for their rapid and reliable identification in medically important samples. the aim of this study was to compare three phenotypic methods for the identification of staphylococcus spp. isolated from patients with urinary tract infection using the pcr of the 16s-23s interspace region generating molecular weight patterns (itr-pcr) as ref ... | 2012 | 23041266 |
| draft genome sequence of staphylococcus saprophyticus subsp. saprophyticus m1-1, isolated from the gills of a korean rockfish, sebastes schlegeli hilgendorf, after high hydrostatic pressure processing. | a bacterium designated m1-1 was isolated from the gills of a korean rockfish, sebastes schlegeli hilgendorf, after high hydrostatic pressure processing. studies of 16s rrna phylogeny and comparative genomics demonstrated that the isolate belongs to staphylococcus saprophyticus subsp. saprophyticus. here, we report the draft genome sequence of s. saprophyticus subsp. saprophyticus m1-1 (kacc 16562). | 2012 | 22843581 |
| potential spread of multidrug-resistant coagulase-negative staphylococci through healthcare waste. | healthcare waste (hcw) might potentially harbor infective viable microorganisms in sanitary landfills. we investigated the antimicrobial susceptibility patterns and the occurrence of the meca gene in coagulase-negative staphylococcus strains (cons) recovered from the leachate of the hcw in an untreated sanitary landfill. | 2015 | 25596568 |
| coagulase-negative staphylococci (cons) isolated from ready-to-eat food of animal origin--phenotypic and genotypic antibiotic resistance. | the aim of this work was to study the pheno- and genotypical antimicrobial resistance profile of coagulase negative staphylococci (cons) isolated from 146 ready-to-eat food of animal origin (cheeses, cured meats, sausages, smoked fishes). 58 strains were isolated, they were classified as staphylococcus xylosus (n = 29), staphylococcus epidermidis (n = 16); staphylococcus lentus (n = 7); staphylococcus saprophyticus (n = 4); staphylococcus hyicus (n = 1) and staphylococcus simulans (n = 1) by phe ... | 2015 | 25475289 |
| characterization of methicillin-resistant coagulase-negative staphylococci (mrcons) in retail meat. | this study was to understand the extent of methicillin-resistant coagulase-negative staphylococci (mrcons) serving as a meca reservoir in retail meat. mrcons were isolated from retail meat (beef, chicken, and turkey) in detroit and characterized by soda gene sequencing for species identification, staphylococcal cassette chromosome mec (sccmec) typing, and pulsed-field gel electrophoresis (pfge). unique mrcons isolates recovered from 25 meat samples were comprised of staphylococcus sciuri (n = 13 ... | 2014 | 24929717 |
| function of grim-19, a mitochondrial respiratory chain complex i protein, in innate immunity. | mitochondria respiratory chain (rc), consisting of five multisubunit complexes, is crucial for cellular energy production, reactive oxygen species generation, and regulation of apoptosis. recently, a few mitochondrial proteins have been reported to be essential for innate immunity, but the function of mitochondrial rc in innate immunity is largely unknown. by knock-out of grim-19, a newly identified subunit protein of mitochondrial complex i, in mice, we found that heterogeneous mice (grim-19(+/ ... | 2012 | 22665480 |
| signal biosynthesis inhibition with ambuic acid as a strategy to target antibiotic-resistant infections. | there has been major interest by the scientific community in antivirulence approaches against bacterial infections. however, partly due to a lack of viable lead compounds, antivirulence therapeutics have yet to reach the clinic. here we investigate the development of an antivirulence lead targeting quorum sensing signal biosynthesis, a process that is conserved in gram-positive bacterial pathogens. some preliminary studies suggest that the small molecule ambuic acid is a signal biosynthesis inhi ... | 2017 | 28607020 |
| using maldi-tof ms typing method to decipher outbreak: the case of staphylococcus saprophyticus causing urinary tract infections (utis) in marseille, france. | staphylococcus saprophyticus is one of the leading causes of urinary tract infections (uti). in december 2014, our surveillance system identified an abnormal increase in s. saprophyticus causing utis in four university hospitals in marseille, indicating a suspected community s. saprophyticus uti outbreak. this was detected by our surveillance system balyses (bacterial real-time laboratory-based surveillance system). s. saprophyticus/ escherichia coli uti ratio increased three-fold from 0.0084 in ... | 2017 | 28831634 |
| [etiology and antimicrobial resistance of uncomplicated urinary tract infections]. | uncomplicated urinary tract infections rank among the most frequent bacterial infections in women in the outpatient setting and represent a major cause of antimicrobial prescription. the aims of this study were to assess frequencies and antimicrobial resistance of current uropathogens causing uncomplicated urinary tract infection. in a prospective multicenter study, patients were recruited in ambulatory settings of four participating hospitals between june 2011 and december 2013. we analyzed 138 ... | 2017 | 28825574 |
| women with symptoms of a urinary tract infection but a negative urine culture: pcr-based quantification of escherichia coli suggests infection in most cases. | our objective was to examine whether or not women with symptoms of a urinary tract infection but with a negative culture (20%-30%) do have an infection. | 2017 | 28457846 |
| prevalence of non-aureus staphylococci species causing intramammary infections in canadian dairy herds. | non-aureus staphylococci (nas), the microorganisms most frequently isolated from bovine milk worldwide, are a heterogeneous group of numerous species. to establish their importance as a group, the distribution of individual species needs to be determined. in the present study, nas intramammary infection (imi) was defined as a milk sample containing ≥1,000 cfu/ml in pure or mixed culture that was obtained from a cohort of cows assembled by the canadian bovine mastitis research network. overall, 6 ... | 2017 | 28527793 |
| staphylococcus aureus nasal colonization differs among pig lineages and is associated with the presence of other staphylococcal species. | staphylococcus aureus is a common colonizer in pigs, with methicillin-resistant s. aureus (mrsa) in particular being a potential health risk to humans. to reduce the exposure to humans, the colonization in pigs should be reduced. the aim of this study was to quantitatively compare the susceptibility of pig lineages for s. aureus colonization, and if the absence of s. aureus could be associated with the presence or absence of other staphylococcal species. nasal samples (n = 129) were obtained fro ... | 2017 | 28691012 |
| galectin-3 is a target for proteases involved in the virulence of staphylococcus aureus. | staphylococcus aureus is a major cause of skin and soft tissue infection. the bacterium expresses four major proteases that are emerging as virulence factors: aureolysin (aur), v8 protease (sspa), staphopain a (scpa), and staphopain b (sspb). we hypothesized that human galectin-3, a β-galactoside-binding lectin involved in immune regulation and antimicrobial defense, is a target for these proteases and that proteolysis of galectin-3 is a novel immune evasion mechanism. indeed, supernatants from ... | 2017 | 28438975 |
| evaluation of lactic acid bacteria isolated from fermented plant products for antagonistic activity against urinary tract pathogen staphylococcus saprophyticus. | urinary tract infections (utis) are the most common infectious diseases in infants and the elderly; they are also the most common among nosocomial infections. the treatment of utis usually involves a short-term course of antibiotics. the purpose of this study was to identify the strains of lactic acid bacteria (lab) that can inhibit the urinary tract pathogen staphylococcus saprophyticus, as alternatives to antibiotics. in this study, we collected 370 lab strains from fermented plant products an ... | 2017 | 28780720 |
| detection of a mecc-positive staphylococcus saprophyticus from bovine mastitis in argentina. | bovine mastitis causes important economic losses in the dairy industry. coagulase-negative staphylococci (cns) are a group of bacteria commonly isolated from bovine mastitis and can display resistance to a wide range of antimicrobial agents. | 2017 | 28732791 |
| antibacterial activity of extract and fractions from branches of protium spruceanum and cytotoxicity on fibroblasts. | the crude ethanol extract (cee) and fractions from branches of protium spruceanum were subjected to antibacterial and cytotoxicity assays. compounds of the most active fraction were identified by gc-ms and lc-ms. cee was active against 19 bacteria and the ethyl acetate fraction (eaf) showed the lowest minimum bactericidal concentration (mbc 0.3-80.0 mg/ml). through time-kill assay was observed that eaf induced rapid bactericidal effect against staphylococcus saprophyticus. the cytotoxicity tests ... | 2017 | 28726498 |
| staphylococcus saprophyticus recovered from humans, food, and recreational waters in rio de janeiro, brazil. | staphylococcus saprophyticus is an important agent of urinary tract infection (uti) in young women, but information about this pathogen in human microbiota and in common environment is lacking. the aim of this study was to characterize s. saprophyticus isolates from genitoanal microbiota of 621 pregnant women, 10 minas cheese packs, and five beaches in rio de janeiro city and compare pfge profiles of these isolates with five uti pfge clusters described in this city. we investigated 65 s. saproph ... | 2017 | 28630628 |
| expression of the stress-response regulators ctsr and hrca in the uropathogen staphylococcus saprophyticus during heat shock. | the uropathogen staphylococcus saprophyticus is an ubiquitous bacterium but little is known about mechanisms that allow its persistence in diverse environments. here we evaluated s. saprophyticus growth and survival during heat shock, the expression of stress response regulators ctsr and hrca through qrt-pcr and heat shock protein synthesis through (35)s-met metabolic labeling. s. saprophyticus does not tolerate temperatures much higher than the optimal 37 °c, as its growth is greatly affected a ... | 2017 | 28455762 |
| genome sequence of staphylococcus saprophyticus dpc5671, a strain isolated from cheddar cheese. | the draft genome sequence of staphylococcus saprophyticus dpc5671, isolated from cheddar cheese, was determined. s. saprophyticus is a common gram-positive bacterium detected on the surface of smear-ripened cheese and other fermented foods. | 2017 | 28428298 |