identification of staphylococcus species by 16s-23s rdna intergenic spacer pcr investigate whether 16s-23s rdna (rdna) spacer region length polymorphisms are suitable for the identification of staphylococcus strains, the 16s-23s rdna intergenic spacer region lengths of 221 strains belonging to 31 species were studied by using a pcr-based method. each species presented a specific 16s-23s pattern made of 1-8 fragments ranging from 104-771 bp, with the exception of the species staphylococcus warnei, staphylococcus caprae and staphylococcus piscifermentans, which presented ...19989734063
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
"staphylococcus pettenkoferi," a novel staphylococcal species isolated from clinical this report we describe a novel species of coagulase-negative novobiocin susceptible staphylococci obtained from an epidemiologically unrelated blood culture and a wound infection. these isolates significantly differed from all other validated staphylococcus species based on phenotypic characteristics and 16s rrna gene sequencing. both isolates had identical 16s rrna sequences and phylogenetic trees constructed from evolutionary distances showed that this species formed a distinct and deep su ...200212106949
microbiology of acute otitis isolate and characterize bacteria and fungi from acute otitis externa (aoe) and to obtain susceptibility profiles on each bacterial isolate.200212169893
inflammatory polyp in the middle ear with secondary suppurative meningoencephalitis in a cat.a 15-month-old male maine coon cat presented with persistent auricular discharge and progressive head tilt, ataxia, and loss of blink on the right side. using computed tomography a hyperattenuating, contrast-enhancing material within a thickened right tympanic bulla and contrast enhancement of the adjacent cerebellum were identified. marked suppurative inflammation was identified on cerebrospinal fluid analysis with no growth on bacterial culture. ventral bulla osteotomy was performed to remove ...200314703245
bacteremia in cirrhotic patients submitted to endoscopic band ligation of esophageal varices.endoscopic procedures can develop bacteremia. patients with chronic liver disease are more predisposed to undergo bacteremia and infections because they are immunocompromised.200415029392
peritoneal dialysis-associated peritonitis caused by staphylococcus auricularis. 200415119644
study of the efficacy of coated vicryl plus antibacterial suture (coated polyglactin 910 suture with triclosan) in two animal models of general evaluate the efficacy in vitro and in vivo of a new antibacterial suture (pgab) compared with a traditional braided suture (pg). our primary goals were to study microbiological effectiveness and impact on wound healing of pgab vs pg. secondary goal was to analyze influence on inflammatory response.200716487594
antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey.the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ...200917475456
staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens.five coagulase-negative, novobiocin-susceptible staphylococcal strains were isolated from human blood cultures in different german and belgian medical facilities. a novel species, 'staphylococcus pettenkoferi' was proposed recently to accommodate two of these strains (b3117(t) and a6664), although the name was not validly published. all five strains belonged to the genus staphylococcus because they were non-motile, gram-positive, catalase-positive cocci with peptidoglycan type (a3 alpha type l-l ...200717625191
early-onset sepsis with staphylococcus auricularis in an extremely low-birth weight infant - an uncommon pathogen.coagulase-negative staphylococci (cons) are often dismissed as a contaminant of blood cultures and are rarely considered as an etiology of perinatally acquired infections. we describe a case of early-onset sepsis with staphylococcus auricularis in an extremely low-birth weight infant.200717653219
inhibitory effect of gram-negative and gram-positive microorganisms against helicobacter pylori clinical determine the in vitro inhibitory effect of several gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates.200817965421
[effectiveness of bacterial screening in preventing and controlling platelet bacterial contamination].this study was purposed to investigate the effectiveness of bacterial screening with 24 hours holding in preventing and controlling bacterial contamination of platelets. bacterial screening of apheresis platelets preserved for 24 hours was performed by using bact/alert automatic bacterial culture system. the samples from 5 bags of platelet were taken in aseptic condition and were merged into 1 bag. the final sample was inoculated into aerobic and anaerobic bottle respectively for testing, meanwh ...200818315928
response to somatic cell count-based selection for mastitis resistance in a divergent selection experiment in sheep.a divergent selection experiment in sheep was implemented to study the consequences of log-transformed somatic cell score (scs)-based selection on resistance to natural intramammary infections. using dams and progeny-tested rams selected for extreme breeding values for scs, we created 2 groups of ewes with a strong divergence in scs of approximately 3 genetic standard deviations. a survey of 84 first-lactation ewes of both the high and low scs lines indicated favorable responses to scs-based sel ...200919233814
metabolism of azo dyes by human skin microbiota.reduction of methyl red (mr) and orange ii (or ii) by 26 human skin bacterial species was monitored by a rapid spectrophotometric assay. the analysis indicated that skin bacteria, representing the genera staphylococcus, corynebacterium, micrococcus, dermacoccus and kocuria, were able to reduce mr by 74-100 % in 24 h, with only three species unable to reduce completely the dye in that time. among the species tested, only corynebacterium xerosis was unable to reduce or ii to any degree by 24 h, an ...201019729456
utilization of umbilical cord blood for the evaluation of group b streptococcal sepsis screening.objective. to evaluate reliability of umbilical cord blood (ucb) for complete blood count (cbc) and blood cultures compared with the infant's blood from peripheral site for group b streptococcal (gbs) sepsis screening. methods. a total of 200 neonates, at risk for gbs infection, were studied prospectively. after birth, ucb sample was obtained for cbc and blood cultures from umbilical vein. peripheral arterial/venous blood was obtained from the neonate. results. in 200 neonates, cbc counts were s ...201122199177
cow teat skin, a potential source of diverse microbial populations for cheese production.the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ...201222081572
conjunctival flora of clinically normal and diseased turtles and captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises.201525889261
next-generation sequencing combined with specific pcr assays to determine the bacterial 16s rrna gene profiles of middle ear fluid collected from children with acute otitis media.the aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged 5 to 42 months (median age, 19 months). the bacteriome profiles of middle ear fluid samples were determined by a nested-pcr amplification of the 16s rrna gene (v4 region), followed by mass sequencing. the prof ...201728357413
external auditory canal microbiology and hearing aid use.the aim of this study was to determine the presence and nature of bacterial flora on hearing aids and the ears of this population. we wonder if the microbiology of the ears with hearing aid wearing differs from the other ear.201323313122
occurrence of cfr-mediated multiresistance in staphylococci from veal calves and pigs, from humans at the corresponding farms, and from veterinarians and their family members.this study reports on the emergence of linezolid-resistant coagulase-negative staphylococci (cons) containing the multiresistance gene cfr in veal calves and pigs, as well as in humans exposed to these animals. cons (staphylococcus auricularis, staphylococcus cohnii, staphylococcus lentus, staphylococcus kloosii, staphylococcus sciuri, staphylococcus simulans), but not staphylococcus aureus, carrying the gene cfr were detected in samples of 12 out of 52 calves at three farms which had a history ...201727102205
isolation of methicillin-resistant staphylococcus spp. from ready-to-eat fish products.a hundred samples from ready-to-eat (rte) fish products were examined for the presence and antimicrobial susceptibility of staphylococcus spp. staphylococci were isolated from 43% of these samples (n = 100). the identified species in the samples were staphylococcus aureus (7%), staphylococcus epidermidis (13%), staphylococcus xylosus (12%), staphylococcus sciuri (4%), staphylococcus warneri (3%), staphylococcus saprophyticus (2%), staphylococcus schleiferi (1%) and staphylococcus auricularis (1% ...201425059796
coagulase-negative staphylococci: pathogenesis, occurrence of antibiotic resistance genes and in vitro effects of antimicrobial agents on biofilm-growing bacteria.coagulase-negative staphylococci (cons) are opportunistic pathogens that particularly cause infections in patients with implanted medical devices. the present research was performed to study the virulence potential of 53 clinical isolates of staphylococcus capitis, staphylococcus auricularis, staphylococcus lugdunensis, staphylococcus simulans, staphylococcus cohnii and staphylococcus caprae. all clinical strains were clonally unrelated. isolates carried genes encoding resistance to β-lactam (me ...201627902368
determination of cd64 for the diagnosis of bacterial chronic prostatitis.cd64 is a 72-kda transmembrane glycoprotein, the highest affinity receptor of immunoglobulin (igg), and a member of the igg superfamily. the cd64 expression by leukocytes in the prostatic fluid was determined by flow cytometry to explore the clinical significance of this glycoprotein in the diagnosis of chronic prostatitis (cp).201525877343
pyrene removal and transformation by joint application of alfalfa and exogenous microorganisms and their influence on soil microbial community.phytoremediation is an attractive approach for the cleanup of polycyclic aromatic hydrocarbons-contaminated soil. the joint effect of alfalfa and microorganisms, including arthrobacter oxydans, staphylococcus auricularis and stenotrophomonas maltophilia, on pyrene removal was investigated. the results showed that the joint effect primarily contributed to pyrene removal, and the concentration of residual pyrene in rhizosphere soil was lower than that in non-rhizosphere soil. after joint treatment ...201425232990
reliability of the microscan walkaway pc21 panel in identifying and detecting oxacillin resistance in clinical coagulase-negative staphylococci strains.the purpose of this study was to determine the reliability of the microscan walkaway poscombo21 (pc21) system for the identification of coagulase-negative staphylococci (cns) strains and the detection of oxacillin resistance. using molecular and phenotypic methods, 196 clinical strains were evaluated. the automated system demonstrated 100 % reliability for the identification of the clinical strains staphylococcus haemolyticus, staphylococcus hominis and staphylococcus cohnii; 98.03 % reliability ...201423887612
two cases of keratosis follicularis squamosa (dohi) caused by swimsuit friction.keratosis follicularis squamosa (kfs) is a keratinizing disorder that is characterized by asymptomatic small scaly patches with follicular plugs scattered on the trunk and thighs. irritation by clothing, bacterial infections, heredity and hormone imbalances have all been proposed as pathogenic factors. case 1 involved an 11-year-old japanese female who presented with a 1-year history of small patches on the lateral chest. she had been attending swimming classes for seven years. a histological ex ...201323608105
antimicrobial activity of daptomycin in comparison to glycopeptides and other antimicrobials when tested against numerous species of coagulase-negative staphylococcus.coagulase-negative staphylococcus spp. (cons) represent a major cause of bloodstream infections, especially in patients with prosthetic devices and intravenous catheters. we evaluated the activity of daptomycin in comparison to vancomycin and teicoplanin against a large collection of 22,024 cons isolates causing clinically significant infections from 283 medical centers over 9 years (2002-2010) and tested for susceptibility by broth microdilution methods against daptomycin and numerous comparato ...201222608137
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