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experimental treatment of neospora caninum-infected mice with the arylimidamide db750 and the thiazolide nitazoxanide.the cationic arylimidamide db750 and the thiazolide nitazoxanide had been shown earlier to be effective against neospora caninum tachyzoites in vitro with an ic(50) of 160nm and 4.23µm, respectively. in this study, we have investigated the effects of db750 and nitazoxanide treatments of experimentally infected balb/c mice, by applying the drugs either through the oral or the intraperitoneal route. in experiment 1, administration of db750 (2mg/kg/day) and nitazoxanide (150mg/kg/day) started alrea ...201121803039
serological response of cats to experimental besnoitia darlingi and besnoitia neotomofelis infections and prevalence of antibodies to these parasites in cats from virginia and pennsylvania.abstract besnoitia darlingi and besnoitia neotomofelis are cyst-forming tissue apicomplexan parasites that use domestic cats ( felis domesticus ) as definitive hosts and opossums ( didelphis virginiana ) and southern plains woodrats ( neotoma micropus ) as intermediate hosts, respectively. nothing is known about the prevalence of b. darlingi or b. neotomofelis in cats from the united states. besnoitia darlingi infections have been reported in naturally infected opossums from many states in the u ...201121506782
epizootiologic investigations of selected abortive agents in free-ranging alpine ibex (capra ibex ibex) in switzerland.in the early 2000s, several colonies of alpine ibex (capra ibex ibex) in switzerland ceased growing or began to decrease. reproductive problems due to infections with abortive agents might have negatively affected recruitment. we assessed the presence of selected agents of abortion in alpine ibex by serologic, molecular, and culture techniques and evaluated whether infection with these agents might have affected population densities. blood and fecal samples were collected from 651 ibex in 14 col ...201121719818
seroepidemiology of toxoplasma gondii, neospora caninum, and leishmania spp. infections and risk factors for cats from brazil.the seroprevalence of infection by toxoplasma gondii, neospora caninum, and leishmania spp. was detected through an indirect immunofluorescence in 70 cats from the andradina municipality, são paulo state, brazil. anti-t. gondii antibodies (titer >64) were detected in 15.7% (11/70) of animals, whereas positivity for n. caninum (titer 16) was not observed in any animal. of the cats from urban and rural areas, 10.4% (5/48) and 27.2% (6/22) were positive for t. gondii, respectively. breed, age, food ...201121626423
first report of neospora caninum infection in cattle in sudan.a cross-sectional survey was conducted in sudan to determine sero-prevalence and risk factors associated with neospora caninum infection in non-vaccinated dairy herds and to assess importance of the disease. blood samples were collected from a total of 262 animals from 25 herds. sera were tested for antibodies against n. caninum using elisa test. the prevalence rates of n. caninum antibodies in cattle were high both at herd level (44%) and at individual animal level (10.7%). herd level infection ...201121898183
the snsag merozoite surface antigens of sarcocystis neurona are expressed differentially during the bradyzoite and sporozoite life cycle stages.sarcocystis neurona is a two-host coccidian parasite whose complex life cycle progresses through multiple developmental stages differing at morphological and molecular levels. the s. neurona merozoite surface is covered by multiple, related glycosylphosphatidylinositol-linked proteins, which are orthologous to the surface antigen (sag)/sag1-related sequence (srs) gene family of toxoplasma gondii. expression of the sag/srs proteins in t. gondii and another related parasite neospora caninum is lif ...201121775062
results of parasitological examinations of faecal samples from cats and dogs in germany between 2003 and 2010.in a retrospective study, the results of parasitological examinations of faecal samples from 8,560 cats and 24,677 dogs between january 2003 and december 2010 in germany were analysed. 30.4 % of the examined dogs and 22.8 % of the cats were infected with endoparasites. the examination of the faecal samples from dogs revealed stages of giardia spp. (18.6 %), toxocara canis (6.1 %), toxascaris leonina (0.6 %), ancylostomatidae (2.2 %), trichuris vulpis (1.2 %), capillaria spp. (1.3 %), crenosoma v ...201121739375
neosporosis in a captive parma wallaby (macropus parma).infection with neospora caninum has been diagnosed in a variety of animal species; however, reports in marsupials are rare. a captive parma wallaby (macropus parma) died suddenly and was subjected to necropsy examination. the main finding was necrotizing myocarditis associated with protozoan parasites. the protozoa were identified as n. caninum by use of immunohistochemistry and partial gene sequence analysis. neospora and toxoplasma should be considered a possible cause of disease in captive ma ...201121689827
seroprevalence of neospora caninum in free-range chickens (gallus domesticus) from the americas.neospora caninum and toxoplasma gondii are biologically and morphologically similar coccidians with canids as definitive hosts for n. caninum and felids for t. gondii. feral chickens have been used as indicators of soil contamination with t. gondii oocysts because they feed from ground. in the present study we studied seroprevalence of n. caninum in free range chickens from different countries in america as an indicator of soil contamination due to n. caninum oocysts. antibodies to n. caninum we ...201121676546
cytokine gene expression at the materno-foetal interface after experimental neospora caninum infection of heifers at 110ôçâdays of gestation.neospora caninum is a major cause of abortion in cattle, but the reasons why only some animals abort remain unclear. the immunological control of the parasite in the placenta or by the foetus could be the key to determining the mechanism of abortion and/or transplacental transmission to the foetus. in this study, cytokine gene expression, analysed by real-time rt-pcr, at the maternal (caruncle) and foetal placenta (cotyledon) of heifers infected at 110ôçâdays of gestation by intravenous inoculat ...201121711362
presence of toxoplasma gondii and neospora caninum dna in the brain of wild birds.toxoplasma gondii infections are prevalent in many avian species and can cause mortality in some bird hosts. although t. gondii has been isolated from various species of birds, the role of many different species of wild birds in the epidemiology of t. gondii remains unknown. neospora caninum, a closely related parasite to t. gondii, has been recently confirmed to infect domestic chickens and wild birds such as house sparrows (passer domesticus). the present study reports the presence of t. gondi ...201121831525
neospora caninum is associated with abortion in algerian cattle.abstract neospora caninum is a major cause of abortion in cattle worldwide. however, little information is available for algeria. accordingly, 799 cattle from 87 farms in the north and northeast of algeria were enrolled in a seroepidemiological survey. an indirect fluorescence antibody test (ifat) revealed a seroprevalence of 19.6%. the animals were divided into 3 groups according to their breed: imported european cattle, local breeds, and crossed animals (european x local). seroprevalences were ...201121728878
a survey of neospora caninum and toxoplasma gondii infection in urban rodents from brazil.abstract toxoplasma gondii is a protozoan parasite that infects humans and other warm blooded animals; it uses feral and domestic cats as the definitive hosts. neospora caninum is a protozoan parasite of animals whose life cycle is very similar to t. gondii, but uses canids as definitive hosts. small rodents play an important role in the life cycle of t. gondii and a few findings indicated that they may be natural intermediate hosts for n. caninum. the present study was aimed at identifying infe ...201121790367
seroprevalence of toxoplasma gondii and neospora caninum in slaughtered pigs in the czech republic.summaryin the czech republic, sera from 551 clinically healthy adult slaughtered pigs (females, 6-8 months old) were collected during the first half of june in 2010. sera were tested for toxoplasma gondii-specific igg antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% s/p were considered as positive. the same samples were also analysed for neospora caninum antibodies using a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30 ...201121819636
neosporosis in animals--the last five years.neospora caninum is a protozoan parasite of animals. until 1988, it was misdiagnosed as toxoplasma gondii. since its first recognition in 1984 in dogs and the description of a new genus and species neospora caninum in 1988, neosporosis has emerged as a serious disease of cattle and dogs worldwide. abortions and neonatal mortality are a major problem in livestock operations and neosporosis is a major cause of abortion in cattle. this review is focused on current status of neosporosis in animals b ...201121704458
prevalence and co-infection of toxoplasma gondii and neospora caninum in apodemus sylvaticus in an area relatively free of cats.summarythe protozoan parasite toxoplasma gondii is prevalent worldwide and can infect a remarkably wide range of hosts despite felids being the only definitive host. as cats play a major role in transmission to secondary mammalian hosts, the interaction between cats and these hosts should be a major factor determining final prevalence in the secondary host. this study investigates the prevalence of t. gondii in a natural population of apodemus sylvaticus collected from an area with low cat densi ...201121756421
presence of antibodies against toxoplasma gondii, neospora caninum and leishmania infantum in dogs from piau+¡.this study aimed to evaluate the presence of antibodies against neospora caninum, toxoplasma gondii and leishmania infantum in dogs attended at the veterinary hospital of the federal university of piau+¡, northeastern brazil, where there are no reports of the occurrence of n. caninum and t. gondii in dogs. serum samples from 530 dogs of genders, different ages and breeds from the municipality of teresina and nearby towns were analyzed using three indirect fluorescent antibody tests, each one tar ...201121722484
Parasite limiting macrophages promote IL-17 secretion in naive bovine CD4? T-cells during Neospora caninum infection.Neospora caninum infects bovine hosts giving rise to pro-inflammatory immune responses that can result in foetal death or spontaneous abortion, this appears to be mediated by the actions of IFN-? on cell activation and migration/trafficking. Yet successful vaccination or natural immunity is also strongly correlated with IFN-? production. We utilised in vitro infection of bovine macrophages to prime naive T-cell responses. Naive T-cells in contact with infected macrophages produce both IFN-? and ...201122005586
identification of a host cell target for the thiazolide class of broad-spectrum anti-parasitic drugs.the thiazolide nitazoxanide (ntz) and some derivatives exhibit considerable in vitro activities against a broad range of parasites, including the apicomplexans neospora caninum and toxoplasma gondii tachyzoites. in order to identify potential molecular targets for this compound in both parasites, rm4847 was coupled to epoxy-agarose and affinity chromatography was performed. a protein of approximately 35 kda was eluted upon rm4847-affinity-chromatography from extracts of n. caninum-infected human ...201121335006
prevalence of neospora caninum antibodies in sheep and goats in pakistan.abstract the purpose of the present study was to obtain seroepidemiological information on the neospora caninum infection status of sheep and goats in different areas of punjab province and azad kashmir (pakistan). a cross-sectional study, using a competitive elisa showed an overall 27.7% (35 of 128) (95% ci ? 7.7%) and 8.6% (13 of 142)(95% ci ? 4.6%) seroprevalence of n. caninum antibodies in sheep and goats, respectively. the difference in seroprevalence between sheep and goat populations wa ...201121854220
anti-neospora caninum antibodies in milk in relation to production losses in dairy cattle.a comprehensive field study was carried out with the following objectives: (a) to assess the usefulness of individual and bulk tank milk analysis for determining neospora caninum serostatus in individual cows and herds, and (b) to study the associations between n. caninum infection status (based on milk testing), and several productive and reproductive parameters in the animals. antibodies were detected with a commercially available elisa test (bio k 192/5). analysis of paired serum and milk sam ...201121645937
Occurrence of antibodies against Neospora caninum and/or Toxoplasma gondii in dogs with neurological signs.This study aimed to evaluate occurrences of antibodies against Neospora caninum and Toxoplasma gondii in dogs with neurological signs. Blood samples from 147 dogs were collected: 127 from owned dogs (attended at the Veterinary Teaching Hospital of the Federal University of Paraná (HV-UFPR) and at private veterinary clinics in the city of Curitiba), and 20 from stray dogs found in Curitiba's metropolitan region. The dogs presented one or more of the following neurological signs: seizures, paresis ...201121961748
implications of wild dog ecology on the sylvatic and domestic life cycle of neospora caninum in australia.neospora caninum is transmitted either transplacentally or horizontally by ingestion of tissue cysts present in tissues or oocysts shed by dogs. neosporosis is a significant disease, causing cattle abortion at 5-7 months of pregnancy. infected cows may remain infective for life transmitting the infection in several consecutive or non-consecutive pregnancies. a great deal is known about the epidemiology of neosporosis, although only limited information is available on the main routes of horizonta ...201120400345
meningoencephalitis associated with sarcocystis spp. in a free-living japanese raccoon dog (nyctereutes procyonoides viverrinus).a free-living, young adult, male japanese raccoon dog (nyctereutes procyonoides viverrinus) was rescued in gifu, japan in march 2009. the animal was weak and emaciated with neurological signs that included head tilt, tremor and tic. the brain showed no gross abnormality at necropsy, but microscopically there was severe meningoencephalitis associated with protozoa, which were morphologically consistent with the asexual developmental stage of sarcocystis spp. the protozoa were immunohistochemicall ...201020163802
stress-driven stage transformation of neospora caninum.neosporiosis, a serious disease caused by the apicomplexan intracellular parasite neospora caninum, is considered to be one of the most economically important diseases in cattle. it is associated with potentially serious complications such as abortion, stillbirth and maternal infertility. to survive in fluctuating physiological and immunological environments, n. caninum has evolved a diverse set of regulatory mechanisms that govern various adaptive responses. the most intriguing paradigm in n. c ...201020165871
comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against besnoitia besnoiti tachyzoites and bradyzoites in bovine sera.besnoitia besnoiti, an apicomplexan parasite causes economically important disease in cattle in many countries of africa and asia is re-emerging in europe. serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. we report new, simplified immunoblot-based serological tests for the detection of b. besnoiti-specific antibodies. antigens were used under non-reducing conditions in ...201020378250
toxoplasma gondii infection specifically increases the levels of key host micrornas.the apicomplexan parasite toxoplasma gondii can infect and replicate in virtually any nucleated cell in many species of warm-blooded animals; thus, it has evolved the ability to exploit well-conserved biological processes common to its diverse hosts. here we have investigated whether toxoplasma modulates the levels of host micrornas (mirnas) during infection.201020090903
the first report of ovine cerebral neosporosis and evaluation of neospora caninum prevalence in sheep in new south wales.presence of neospora caninum dna was detected in the brain and spinal cord of an adult merino sheep suspected of dying with acute non-suppurative meningoencephalitis and mild to moderate non-suppurative myelitis. the most severe neurological lesions were found in the midbrain at the rostral coliculi with moderate to severe multifocal vasculitis and gliosis. as this was the first known occurrence of cerebral disease in sheep in australia caused by n. caninum, we surveyed sera from five sheep prop ...201020189309
neospora caninum: application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in balb/c mice.liposomes coated with neoglycolipids constructed with mannopentaose and dipalmitoylphosphatidylethanolamine (m3-dppe), referred to as m3-dppe liposomes, have been shown to induce cellular immunity against antigens encapsulated therein. to evaluate whether these m3-dppe liposomes have an adjuvant capacity against neospora caninum infection, a novel immunization method utilizing soluble n. caninum apical membrane antigen 1 (ncama1) encapsulated in the m3-dppe liposomes (m3-ncama1) was employed. th ...201020097198
detection of anti-toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by indirect fluorescence assay (ifa) and indirect elisa.the indirect fluorescence assay (ifa) and the indirect elisa were comparatively used to detect igg and igm antibodies for toxoplasma gondii in experimentally and naturally infected primates. in the experimentally infected group, antibodies of diagnostic value were detected at day 9 post-infection (pi) with the ifa (igg and igm) and with igg-elisa. igm-elisa detected antibodies for t. gondii starting at day 3 pi until the end of the experiment (102 days pi). of the 209 naturally infected sera tes ...201020385056
prevalence of antibodies to trypanosoma cruzi, leishmania infantum, encephalitozoon cuniculi, sarcocystis neurona, and neospora caninum in capybara, hydrochoerus hydrochaeris, from são paulo state, brazil.little is known about the importance of capybara, hydrochoerus hydrochaeris, as reservoirs for parasites of zoonotic or veterinary importance. sera from 63 capybaras, from 6 counties in the state of são paulo, brazil, were examined for antibodies to trypanosoma cruzi, leishmania infantum, encephalitozoon cuniculi, sarcocystis neurona, and neospora caninum using an indirect immunofluorescent antibody test. five (8%) of the 63 capybaras had antibodies to t. cruzi epimastigotes. none of the samples ...201020020808
conventional and molecular diagnostic testing for the acute neurologic patient.the aim of this review is to describe and evaluate both conventional and molecular diagnostic testing utilized in dogs and cats with acute neurologic diseases. various types of polymerase chain reaction (pcr) are explored along with novel molecular diagnostic testing that ultimately may prove useful in the critical care setting.201020230434
pathogenic characterization in mice of neospora caninum isolates obtained from asymptomatic calves.in this study, we characterized 8 new isolates obtained from healthy but congenitally infected calves using a balb/c mouse model. neospora caninum-infected mice survived without exhibiting any clinical signs of disease. nevertheless, differences among isolates in parasite organ distribution, parasite burden and the severity of histopathological lesions were determined. mice infected with the nc-spain 5h, nc-spain 7 and nc-spain 9 isolates showed higher parasite burdens and more severe brain lesi ...201020233488
necrotizing cerebellitis and cerebellar atrophy caused by neospora caninum infection: magnetic resonance imaging and clinicopathologic findings in seven dogs.adult dogs with neosporosis can develop a variety of neurologic signs. no area of predilection within the nervous system so far has been identified in adult dogs.201020337912
neospora caninum and toxoplasma gondii antibodies in european brown hares in the czech republic, slovakia and austria.european brown hares (lepus europaeus) coming from the czech republic, slovakia and austria were tested for serum antibodies against neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and for serum antibodies against toxoplasma gondii by an indirect fluorescent antibody test. in a total of 925 samples, 280 (30%) and 132 (14%) reacted positively for n. caninum and t. gondii antibodies, respectively. out of 333 hares in the czech republic, 129 (39%) and 71 (21%) reacted ...201020347525
a survey of neospora caninum-associated bovine abortion in large dairy farms of mashhad, iran.neospora caninum is an apicomplexan protozoon causing abortion in cattle worldwide. the present study was designed to assess the importance of this parasite for causing abortion in dairy farms in the mashhad area of iran. of the aborted bovine fetuses, 151 were collected from dairy farms between 2006 and 2008. first, brain samples were examined by polymerase chain reaction (pcr) for the presence of n. caninum dna, diagnosis was complemented with immunohistochemistry (ihc) and fetal serology (eli ...201020352453
development of an indirect elisa test using an affinity purified surface antigen (p38) for sero-diagnosis of canine neospora caninum infection.neospora caninum is an intracellular protozoan that infects domestic and wild canids, as well as many warm blooded animals as shown by the isolation of viable parasites and the detection of parasite dna in naturally infected hosts. n. caninum is described as a cause of neuromuscular disease and death in dogs. the dog is also known as a definitive host, shedding oocysts involved in the transmission of the infection to intermediate hosts. this study was conducted to develop an indirect elisa test ...201020434268
molecular characterization of neospora caninum mag1, a dense granule protein secreted into the parasitophorous vacuole, and associated with the cyst wall and the cyst matrix.summary: in neospora caninum and toxoplasma gondii, the parasitophorous vacuole (pv) is synthesized at the time of infection. during tachyzoite-to-bradyzoite stage conversion, the pv is later transformed into a tissue cyst that allows parasites to survive in their host for extended periods of time. we report on the characterization of ncmag1, the n. caninum orthologue of t. gondii mag1 (matrix antigen 1; tgmag1). the 456 amino acid predicted ncmag1 protein is 54% identical to tgmag1. by immunobl ...201020444303
occurrence of antibodies anti-neospora caninum, anti-toxoplasma gondii, and anti-leishmania chagasi in serum of dogs from pará state, amazon, brazil.a cross-sectional study was conducted to determine the occurrence of anti-toxoplasma gondii, anti-neospora caninum, and anti- leishmania chagasi antibodies in dogs of the state of pará, brazil. for this purpose, 129 blood samples were collected from dogs of different ages and gender. samples of 72 dogs were collected from 39 rural properties from 19 municipalities, and 57 samples were from stray dogs, collected after captivity by the center of zoonosis control from the municipality of santarém. ...201020445991
roles of cd122+ cells in resistance against neospora caninum infection in a murine model.innate cells, such as natural killer (nk) cells and nkt cells, play essential roles as primary effector cells at the interface between the host and parasite until establishment of adaptive immunity. however, the roles of nk and nkt cells in defense against neospora caninum have not been well clarified. nk and nkt cells were depleted by the treatment with an anti-cd122 (interleukin-2 receptor beta chain) monoclonal antibody (mab, tm-β1) in vivo. the parasite burden in the brain of mice was promot ...201020460838
australian dingoes are definitive hosts of neospora caninum.to provide objective data on the potential role of dingoes (canis lupus dingo) in the life cycle of neospora caninum in australia, the production of n. caninum oocysts by experimentally infected canids was investigated. three dingo pups raised in captivity and three domestic dogs were fed tissue from calves infected with an australian isolate of n. caninum, nc-nowra. oocysts of n. caninum, confirmed by species-specific pcr, were shed in low numbers by one dingo pup at 12-14 days p.i. the remaini ...201020149793
detection of neospora caninum dna in capybaras and phylogenetic analysis.the role of rodents in the sylvatic cycle of neospora sp. and in the neosporosis epidemiology is still uncertain. the aim of the present work was to detect neospora caninum and to determine its prevalence in capybaras (hydrochaeris hydrochaeris), to help elucidate the role of this rodent in the life cycle of the parasite. n.caninum dna was detected by pcr using 4 different sets of primers specific to the nc5 and its1 sequences. the parasite was found in the lymph nodes, heart, liver, and blood o ...201020470895
recognition by toll-like receptor 2 induces antigen-presenting cell activation and th1 programming during infection by neospora caninum.neospora caninum is an apicomplexan parasite responsible for major economic losses due to abortions in cattle. toll-like receptors (tlrs) sense specific microbial products and direct downstream signaling pathways in immune cells, linking innate, and adaptive immunity. here, we analyze the role of tlr2 on innate and adaptive immune responses during n. caninum infection. inflammatory peritoneal macrophages and bone marrow-derived dendritic cells exposed to n. caninum-soluble antigens presented an ...201020404835
detection of toxoplasma gondii, neospora caninum, and encephalitozoon cuniculi in the brains of common voles (microtus arvalis) and water voles (arvicola terrestris) by gene amplification techniques in western austria (vorarlberg).knowledge about the protozoan parasite fauna in voles (arvicolinae) in austria is rather limited, although some of these pathogens play an important role in human medicine and cause zoonoses (e.g., toxoplasma gondii and encephalitozoon cuniculi). others are of relevance in veterinary medicine and have a negative economic impact (e.g., neospora caninum). two hundred sixty-eight common voles (microtus arvalis) and 86 water voles (arvicola terrestris) from the most western austrian province, vorarl ...201020480373
neospora spp. and toxoplasma gondii antibodies in horses in the czech republic.during january 2007, blood samples were collected from 552 healthy horses from nine different regions of the czech republic. sera were tested for serum antibodies to neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. the same samples were tested for serum antibodies against toxoplasma gondii by a latex agglutination test. in total, 131 of 552 (24%) horses reacted positively for neospora antibodies in competitive- ...201020532561
neospora caninum: in vitro culture of tachyzoites in mcf-7 human breast carcinoma cells.the development of neospora caninum tachyzoites, an apicomplexan protozoan parasite, was studied in vitro using the human breast carcinoma cell 7 (mcf-7) as the host cell line. the extracellular nc-1 tachyzoites in mcf-7 cells were observed and counted daily for 6 consecutive days post-infection to establish the growth curve. the intracellular parasites were observed by acridine orange staining using laser scanning confocal microscope. the results indicated that nc-1 tachyzoites invaded mcf-7 ce ...201020566363
microsatellite typing and avidity analysis suggest a common source of infection in herds with epidemic neospora caninum-associated bovine abortion.neosporosis is an important cause of reproductive failure in cattle worldwide. two different abortion patterns associated with neospora caninum infection have been observed in cattle herds: endemic and epidemic abortion outbreaks. the endemic pattern is characterized by an abortion problem in a herd persisting for several months or years, and is assumed to be caused by reactivation of a chronic infection. in epidemic outbreaks, abortions concentrate within a short period of time, most likely due ...201020609521
seroprevalence of neospora caninum in gray wolves in scandinavia.transmission of the protozoan parasite neospora caninum between wild and domestic animals has gained some interest during recent years. because of the close relationship between gray wolf (canis lupus) and dog it has been suggested that gray wolf is a definitive host for the parasite. the aim of this study was to estimate the seroprevalence of n. caninum in scandinavian gray wolves and to investigate any geographical patterns of the infection. the investigation was based on blood samples collect ...201020609524
prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in swine from northeastern brazil.a serologic survey was conducted among 130 swine slaughtered in the public slaughterhouse of the city of patos, paraíba state, northeastern brazil, to determine the prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies, and to verify possible associations between sex of the animals and antibody prevalence. the sera were analyzed by indirect antibody tests, considering 1:64 (t. gondii) and 1:50 (n. caninum) dilutions as cut-off points. the prevalence of anti-t. gondii antibodi ...201020624342
association between seropositivity for neospora caninum and reproductive performance of beef heifers in the pantanal of mato grosso do sul, brazil.neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, ruminants, and horses. the purpose of this study was to evaluate the association between the presence of antibodies to n. caninum and reproductive loss in beef heifers in the south pantanal region of mato grosso, brazil. a total of 1098 heifers were evaluated from breeding to calving with regard to reproductive performance, and serology for neosporosis was assayed by indirect immunofluorescence react ...201020624350
neospora caninum: cloning and expression of a gene coding for cytokine-inducing profilin.profilins are actin-binding proteins that in toxoplasma gondii stimulate innate immunity in mice by binding toll-like receptors (tlr) on dendritic cells (dc) leading to release of inflammatory cytokines, primarily il-12 and ifn-gamma. the purpose of the present study was to characterize neospora caninum profilin, termed ncprofilin. recombinant ncprofilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native ncprofilin antigen in n. can ...201020211619
the prevalence and spatial clustering of neospora caninum in dairy herds in norway.the aims of this study were to estimate the national herd prevalence and the general spatial distribution of neospora caninum infection in norwegian dairy cattle. bulk milk samples from 1657 randomly selected dairy herds were analysed by iscom elisa for the presence of n. caninum specific antibodies. the national herd prevalence was estimated to be 0.7% (95% ci: 0.3-1.2%), which is the lowest national herd prevalence reported in europe. in addition, we included a subpopulation study in which all ...201020227188
association between neospora caninum seropositivity and perinatal mortality in dairy heifers at first calving.associations between neospora caninum infection and the reproductive performance of dairy heifers in their first and second pregnancy on 18 uk farms were examined. six-month-old heifer calves were tested for n caninum-specific antibodies with a commercial elisa, and were then monitored until their second calving. random-effects regression analyses were performed on the data, using serological status as the explanatory variable. of 460 heifers tested, 7.2 per cent were seropositive. seropositive ...201020643884
abortions in bovines and neospora caninum transmission in an embryo transfer center.neosporosis is considered to be one of the main causes of abortions in bovines. in this study we evaluated the congenital transmission and occurrence of abortions by neospora caninum in an embryo transfer center in neropolis, goiás. serological samples from 101 recipients, 61 donors, and 90 calves were analyzed. among these animals, 32.67% of the recipients, 22.22% of the donors, and 6.66% of the calves were positive for n. caninum. the rate of vertical transmission was 24%. there was a statisti ...201020655663
rng1 is a late marker of the apical polar ring in toxoplasma gondii.the asexually proliferating stages of apicomplexan parasites cause acute symptoms of diseases such as malaria, cryptosporidiosis and toxoplasmosis. these stages are characterized by the presence of two independent microtubule organizing centers (mtocs). centrioles are found at the poles of the intranuclear spindle. the apical polar ring (apr), a mtoc unique to apicomplexans, organizes subpellicular microtubules which impose cell shape and apical polarity on these protozoa. here we describe the c ...201020658557
neospora caninum and toxoplasma gondii antibody prevalence in alaska wildlife.free-ranging caribou and moose populations in some regions of alaska undergo periodic declines in numbers. caribou and moose are managed by the state as valuable resources for not only sustenance and subsistence, but also for cultural heritage. incidence and prevalence of diseases that may impact herd health and recruitment from year to year are relevant to management decisions aimed to protect the long-term viability of these herds. neospora caninum and toxoplasma gondii are two apicomplexan pa ...201020688628
kennel dogs as sentinels of leishmania infantum, toxoplasma gondii, and neospora caninum in majorca island, spain.kennel dogs can serve as sentinels and/or reservoirs of diseases of veterinary and zoonotic interest because they have often roamed free and lived outdoors, thus being exposed to pathogens. we tested dogs from the kennel of inca (majorca/mallorca, balearic islands, spain) for evidence of infection with three protozoan parasites: leishmania infantum, toxoplasma gondii, and neospora caninum. exposure to l. infantum was found in 56.3% of 48 dogs (37.5% by western blot, 43.8% by pcr). only 30% of in ...201020689966
neospora caninum and coxiella burnetii seropositivity are related to endocrine pattern changes during gestation in lactating dairy cows.q fever is a zoonotic infection caused by coxiella burnetii that is endemic worldwide. domestic ruminants are a source of infection for humans. given the suggestion that the bacterium recrudesces during pregnancy in cattle, this study was designed to determine whether c. burnetii infection affects hormonal patterns, such as progesterone, cortisol, pregnancy associated glycoproteins (pag), and prolactin during gestation in lactating cows. possible interactions with neospora caninum were also expl ...201020416940
comparative analysis of stage specific gene regulation of apicomplexan parasites: plasmodium falciparum and toxoplasma gondii.apicomplexans comprise some of the most life threatening parasites infecting human and livestock and includes plasmodium and toxoplasma, the causative agents of malaria and toxoplasmosis respectively, in humans as well as neospora caninum (abortion in livestock, neosporosis in dogs), cryptosporidium (diarrheal cryptosporidiosis and opportunistic infections in aids patients) and eimeria (poultry coccidiosis). these parasites are characterized by a complex life cycle usually alternating between se ...201020429866
neospora caninum: evaluation of vertical transmission in slaughtered beef cows (bos indicus).neospora caninum is a protozoan parasite that causes the most important reproductive problems in cattle worldwide. the objective of this study was to evaluate the possibility of vertical transmission of n. caninum in zebus breed beef cows (bos indicus) submitted for slaughter at an abattoir in the northern region of the state of paraná, southern brazil. one hundred and fifty-nine cows were evaluated: 83 pregnant (in different stages of gestation) and 76 non-pregnant. serum determination of n. ca ...201021063729
influence of adjuvant and antigen dose on protection induced by an inactivated whole vaccine against neospora caninum infection in mice.in this study, the protection afforded by a neospora caninum inactivated vaccine formulated with three different adjuvants (water-in-oil emulsion, aluminum hydroxide with cpg oligodeoxynucleotides and aluminum hydroxide with ginseng extract) and three different parasite doses (10(5), 5 × 10(5) or 10(6) inactivated whole tachyzoites) was evaluated using a mouse model. mice were immunized subcutaneously twice at three-week intervals with inactivated nc-spain 1h tachyzoites and challenged by intrap ...201021067865
a second generation multiplex pcr for typing strains of neospora caninum using six dna targets.genetic diversity of neospora caninum was investigated through a study of repetitive sequences found in the genome of this species. twenty different loci were studied, and three were identified that varied in repeat content amongst isolates. no relationship was found between the copy number of repetitive sequences present and host type or geographical location from which the isolates were derived. a multiplex pcr assay was developed for multilocus-strain typing using three microsatellites and th ...201019683051
plasmacytoid and conventional dendritic cells are early producers of il-12 in neospora caninum-infected mice.neospora caninum is a coccidian parasite causative of clinical infections in a wide range of animal hosts. the maturation and activation of splenic conventional dendritic cells (cdcs) and plasmacytoid dendritic cells (pdcs) were studied here in balb/c mice challenged intraperitoneal with n. caninum tachyzoites. the number of cdcs was found to decrease in the spleen of the infected mice 12 h and 2 days after the parasitic challenge, whereas at day 5 after infection it was significantly above that ...201019755980
risk factors associated with neospora caninum seropositivity in sheep from the state of alagoas, in the northeast region of brazil.the aim of this study was to identify risk factors associated with neospora caninum seropositivity in sheep from the state of alagoas, in the northeast region of brazil. twenty-six herds were selected, and blood samples were collected from 343 animals > 6-mo-old. the indirect fluorescent antibody test (ifat) was used for serological diagnosis of n. caninum infection. epidemiological questionnaires were applied for each herd to identify the risk factors associated with infection. thirty-three (9. ...201019799489
seroprevalence of antibodies to neospora caninum in bos javanicus ('bali cattle') from indonesia.a cross-sectional survey was performed to obtain first information on the presence of neospora caninum infection in bos javanicus ('bali cattle'), the predominant beef cattle in the eastern islands of indonesia. serum samples were collected from 438 bali cattle of two age classes (<2 years, >2 years) and both genders at three slaughterhouses in the bali island, and examined for n. caninum-specific antibodies using native ncsrs2 (p38 antigen) as an elisa antigen. the estimated overall seroprevale ...201019533406
members of a novel protein family containing microneme adhesive repeat domains act as sialic acid-binding lectins during host cell invasion by apicomplexan parasites.numerous intracellular pathogens exploit cell surface glycoconjugates for host cell recognition and entry. unlike bacteria and viruses, toxoplasma gondii and other parasites of the phylum apicomplexa actively invade host cells, and this process critically depends on adhesins (microneme proteins) released onto the parasite surface from intracellular organelles called micronemes (mic). the microneme adhesive repeat (mar) domain of t. gondii mic1 (tgmic1) recognizes sialic acid (sia), a key determi ...201019901027
first identification of neospora caninum by pcr in aborted bovine foetuses in romania.the first identification of neospora caninum infection in aborted bovine foetuses in romania is reported. nine aborted foetuses were collected from a dairy farm. the foetal age of foetuses was between 3 and 7 months. a 7-month-old foetus was mummified. n. caninum dna nc-5 region was amplified from samples extracted from brain tissues of three (33.3%) aborted foetuses. the foetal ages of pcr positive foetuses were 3, 4 and 7 months (mummified). no specific lesions or tissue cysts were found to th ...201019953273
protection against lethal neospora caninum infection in mice induced by heterologous vaccination with a mic1 mic3 knockout toxoplasma gondii strain.neospora caninum and toxoplasma gondii are closely related, obligate intracellular parasites infecting a wide range of vertebrate hosts and causing abortion and neonatal morbidity and mortality. several lines of evidence suggest that cross immunity between these two pathogens could be exploited in the design of strategies for heterologous vaccination. we assessed the ability of an attenuated strain of t. gondii ("mic1-3ko strain") conferring strong protection against chronic and congenital toxop ...201019995895
toxoplasma gondii and neospora caninum in sparrows (passer domesticus) in the northeast of brazil.toxoplasma gondii is a cosmopolitan protozoan parasite of warm-blooded animals that causes high rates of infection in mammals and birds. sparrows (passer domesticus) are synantropic birds which are distributed worldwide. they serve as intermediate hosts for the parasite but are quite resistant to toxoplasmosis. the aims of this study were to determine the frequency of t. gondii infection in sparrows using serologic and molecular tests, and to investigate related parasites, such as neospora canin ...201019879051
seroprevalence of neospora caninum infection in dairy cattle in northeastern china.the seroprevalence of neospora caninum infection in china's northeastern heilongjiang province was surveyed between 2007 and 2008. in total, 540 serum samples of dairy cattle from 9 counties were examined for antibodies to n. caninum by enzyme-linked immunosorbent assay (elisa). the overall seroprevalence of n. caninum in dairy cattle was 13.3% (72/540). the seroprevalence of n. caninum in aborting cows (14.9%) was slightly higher than that in nonaborting cows (10.3%). dairy cattle with 5 pregna ...201019895158
flock-level seroprevalence of, and risk factors for, neospora caninum among sheep and goats in northern jordan.during the period january 2002 to december 2003, serum samples were collected from 104 small ruminant flocks consisting of 18 sheep flocks, 27 goat flocks and 59 mixed flocks containing both sheep and goats in northern jordan. only female animals were sampled. at least 5 females aged over 2 years per flock per species were sampled and examined for anti-neospora caninum antibodies using elisa. to increase the chances of detecting positive flocks, sick or older ewes were sampled. also, n. caninum ...201019923025
some factors affecting the abortion rate in dairy herds with high incidence of neospora-associated abortions are different in cows and heifers.the aim of this study was to determine if the factors affecting the abortion rate in dairy herds with high incidence of neospora-associated abortions are different in pregnancies of cows and heifers chronically infected with neospora caninum. in heifers (n = 229), an increase in the cumulative number of days with a mean relative humidity (rh) lower than 60% during the second trimester of gestation increases the risk of abortion. yet, the likelihood of abortion was 7.6 times lower for pregnant he ...201019210662
a4d12 monoclonal antibody recognizes a new linear epitope from sag2a toxoplasma gondii tachyzoites, identified by phage display bioselection.toxoplasma gondii surface is coated by closely related antigens that belong to srs (sag-1 related sequences) superfamily. two tachyzoite-specific srs antigens, sag1 and sag2, are immunodominant proteins that apparently modulate the virulence of infection by inducing the host immune response against tachyzoites during the acute phase. in this study, we described a conformationally insensitive monoclonal antibody (a4d12mab) that recognizes a linear epitope shared by two isoforms of p22 that is exp ...201019261354
seroprevalence of toxoplasma gondii and neospora caninum in wild and domestic ruminants sharing pastures in galicia (northwest spain).the prevalence of antibodies to the protozoan parasites toxoplasma gondii and neospora caninum were investigated by the direct agglutination test (dat) and celisa, respectively, in 160 roe deer (capreolus capreolus), 177 sheep and 178 cattle sharing pastures in galicia (northwest spain). the seroprevalence for t. gondii was 13.7% in roe deer, 57% in sheep and 7.3% in cattle. the seroprevalence for n. canimum was 6.8%, 10.1% and 24.1% in roe deer, sheep and cattle, respectively. statistically sig ...201019482324
influence of neospora caninum intra-specific variability in the outcome of infection in a pregnant balb/c mouse model.previous assays in pregnant animals have demonstrated the effect of different host factors and timing of infection on the outcome of neosporosis during pregnancy. however, the influence of neospora caninum isolate itself has been poorly investigated. here, we compared the effects on clinical outcome and vertical transmission observed in a pregnant mouse model following infection with 10 different n. caninum isolates. the isolates in our study included the nc-liv isolate and nine n. caninum isola ...201020416260
clinical and clinicopathological features of non-suppurative meningoencephalitis in young greyhounds in ireland.the clinical and clinicopathological features of non-suppurative meningoencephalitis in 30 greyhounds were reviewed. the dogs were from 21 separate litters, comprised both sexes (16 males and 14 females) and ranged in age from five to 18 months. in 14 (66.7 per cent) litters, more than one case was suspected or confirmed, and the number of siblings affected within individual litters ranged from one to seven. clinical signs were progressive and varied from five days to 12 months in duration; 12 d ...201020802187
microarray analyses of mouse responses to infection by neospora caninum identifies disease associated cellular pathways in the host response.neospora caninum is a coccidian cyst-forming parasite found in a wide range of host species such as mice, dogs and cattle. the development of methods such as vaccines to prevent abortion and fetal loss due to neosporosis would be greatly assisted by further knowledge on immunity and host responses to infection. in this study we used microarray technology to investigate the protective host responses occurring at 6h post infection in the spleen of mice infected with a prototype live n. caninum vac ...201020817048
neuritis of the cauda equina in a dog.this study presents the first case report of neuritis of the cauda equina in a dog, including characterisation of the inflammatory infiltrate. the dog in question, a 6-year-old welsh springer spaniel, was presented with flaccid tail and faecal and urinary incontinence. the histological lesions included severe mononuclear cell infiltration of the nerve roots of the cauda equina and of the lumbar nerve roots. the infiltrate was composed of large numbers of t-lymphocytes and b-lymphocytes and small ...201020840297
neospora caninum infection in stray and farm dogs: seroepidemiological study and oocyst shedding.in a previous study, farm and stray dogs were considered potential high risk populations of neospora caninum infection in spain. consequently, we decided to investigate the significance of n. caninum infection in these populations. specific antibodies were detected in 120 out of 275 dog sera (43.6%), with titres ranging from 1:50 to 1:800. differences in seroprevalence between farm (47.5%, 67/141) and stray (39.5%, 53/134) dogs were not significant (p>0.05; χ(2) test), but farm dogs showed signi ...201020851523
a new thrombospondin-related anonymous protein homologue in neospora caninum (ncmic2-like1).neospora caninum is an apicomplexan protozoan that has the dog as a definitive host and cattle (among other animals) as intermediate hosts. it causes encephalopathy in dogs and abortion in cows, with significant loss in worldwide livestock. as any apicomplexan, the parasite invades the cells using proteins contained in the phylum-specific organelles, like the micronemes, rhoptries and dense granules. the aim of this study was the characterization of a homologue (denominated ncmic2-like1) of n. c ...201020880420
update on the diagnosis and management of neospora caninum infections in dogs. 201020937501
prevalence of neospora caninum antibodies in sheep flocks of uberlândia county, mg.neosporosis is a parasitic disease that occurs in sheep and is associated with reproductive failure. the aim of this study was to verify seroprevalence of neospora caninum antibodies in 12 sheep flocks in uberlândia county, minas gerais, brazil, using indirect immunofluorescence assay (ifa). a total of 334 sheep blood samples were analyzed and an epidemiologic questionnaire was applied for each farm in order to correlate with risk factors of neosporosis: gender, age, breed, abortion problems, co ...201020943017
antibodies to toxoplasma gondii and neospora caninum in captive neotropical and exotic wild canids and felids.this study was designed to detect antibodies to toxoplasma gondii and neospora caninum in wild captive carnivores maintained in brazilian zoos. blood samples were collected from 142 brazilian wild felids and 19 exotic felids in zoos, and 3 european wolves (canis lupus) and 94 brazilian wild canids maintained in captivity in brazilian zoos of são paulo, mato grosso states and federal district. one hundred and two (63.4%) and 70 (50.3%) of the 161 wild felids tested were seropositive for t. gondii ...201020950109
effects of neospora caninum infection at mid-gestation on placenta in a pregnant mouse model.neospora caninum is one of the more-efficient transplacentally-transmitted organisms. the goal of the present study was to investigate the pathologic and immunologic changes that occur at the materno-fetal interphase in pregnant balb/c mice infected with n. caninum at mid-gestation. parasite dna was detected in feto-placentary units 3 days post-infection (pi). on day 7 pi, the dna detection level and parasite burden were significantly higher in the placentas than in the fetuses, which may indica ...201020950112
validation of a leishmania infantum elisa rapid test for serological diagnosis of leishmania chagasi in dogs.canine visceral leishmaniasis (cvl) is caused by leishmania donovani complex parasites including l. donovani, leishmania infantum and leishmania chagasi. as some studies suggest that l. chagasi and l. infantum may be very similar or even the same species, the aim of the present study was to evaluate a commercial rapid elisa test, originally designed for l. infantum, in the diagnosis of cvl in dogs naturally infected by l. chagasi. a total of 400 serum canine samples, including 283 positive dogs ...201021030153
identification of hammondia heydorni oocysts by a heminested-pcr (hnpcr-ap10) based on the h. heydorni rapd fragment ap10.toxoplasma gondii, hammondia hammondi, neospora caninum, neospora hughesi and hammondia heydorni are members of the toxoplasmatinae sub-family. they are closely related coccidians with similarly sized oocysts. molecular diagnostic techniques, especially those based on polymerase chain reaction (pcr), can be successfully applied for the differentiation of hammondia-like oocysts. in this paper, we describe a rapid and simple method for the identification of h. heydorni oocysts among other members ...201021030154
evaluation of a commercial elisa for the specific detection of antibodies against besnoitia besnoiti.bovine besnoitiosis is an economically important disease in cattle caused by the protozoan parasite besnoitia besnoiti, which occurs endemically in many countries of africa and asia and is spreading in europe. serological identification of subclinically infected cattle is important to avoid the introduction of infected animals into naive herds. here we determine the sensitivity and specificity of the priocheck(®) besnoitia ab, a serological test recently introduced into the european market. anal ...201021035269
crystallization and preliminary x-ray structural analysis of nucleoside triphosphate hydrolases from neospora caninum and toxoplasma gondii.the nucleoside triphosphate hydrolases that are produced by neospora caninum (ncntpase) and toxoplasma gondii (tgntpase-i) have a different physiological function from the ubiquitous ecto-atpases. the recombinant enzymes were crystallized at 293 k using polyethylene glycol 3350 as a precipitant and x-ray diffraction data sets were collected for ncntpase (to 2.8 å resolution) and tgntpase-i (to 3.1 å resolution) at 100 k using synchrotron radiation. the crystals of ncntpase and tgntpase-i belonge ...201021045291
immune response to neospora caninum native antigens formulated with immune stimulating complexes in calves.the aim of this study was to compare the immune responses to live neospora caninum tachyzoites and n. caninum native antigens formulated with immune stimulating complexes matrix (iscom-matrix) in calves. fifteen calves were used in this study: 3 were intravenously inoculated with 1 × 10(8) live tachyzoites (group a), 3 were inoculated twice with n. caninum native antigens formulated with iscoms (group b); 3 with n. caninum native antigens in phosphate-buffered saline (pbs) (group c); 3 received ...201021074325
evaluation of toxoplasma gondii and neospora caninum infections in sheep from uberlândia, minas gerais state, brazil, by different serological methods.toxoplasmosis and neosporosis have been recognized as economically important diseases with considerable impact on the livestock industry. considering the scarce information on the occurrence of toxoplasma gondii and neospora caninum infections in sheep from uberlândia, minas gerais state, brazil, this study aimed to investigate the frequency of antibodies against these parasites in sheep sera from this region by using different serological methods. a total of 155 sheep serum samples were analyze ...201021075529
prevalence of antibodies against neospora caninum in dogs from urban areas in central poland.neospora caninum is a protozoan parasite which causes abortion in cattle as well as reproduction problems and neurological disorders in dogs. to assess the prevalence of the parasite in urban dogs in the mazovian voivodeship, central poland, serum samples from 257 dogs were analyzed for the presence of specific igg antibodies. the examined dogs visited three private veterinary clinics located in warsaw due to control tests, vaccinations, or other reasons not directly connected with neosporosis. ...201021079995
in vitro isolation and identification of the first neospora caninum isolate from european bison (bison bonasus bonasus l.).peripheral blood from european bison (bison bonasus bonasus l.) living in białowieża forest, north-east poland, were investigated for the presence of antibodies to neospora caninum and isolation of parasite. out of 23 animals three of them showed a strong positive response to n. caninum (13%). the white blood cells from two positive and two negative bison were loaded on monolayer vero cells culture. the first viable tachyzoites were detected only in positive samples at days 60 and 70 after incub ...201020702042
prevalence of toxoplasma gondii and neospora caninum antibodies in spanish ibex (capra pyrenaica hispanica).a cross-sectional study was carried out on spanish ibex populations in southern spain to assess the seroprevalence of toxoplasma gondii and neospora caninum and to investigate the risk factors associated with these infections. using the modified agglutination test, the seroprevalence to t. gondii was 27.5% (146/531; ci(95%), 23.7-31.3), and this seropositivity significantly increased with age. among adults, statistically significant differences were observed between geographical locations and ov ...201021159529
construction of neospora caninum stably expressing tgsag1 and evaluation of its protective effects against toxoplasma gondii infection in mice.toxoplasma gondii and neospora caninum are closely related apicomplexan parasites. the surface antigen 1 of t. gondii (tgsag1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. in this study, n. caninum stably expressing the tgsag1 gene (nc/tgsag1) was constructed using pyrimethamine-resistant dhfr-ts and gfp genes as double-selection markers. the expression level, molecular weight ...201020832493
seroprevalence of neospora caninum in free living and farmed red deer (cervus elaphus) in poland.serum samples from 47 free living and 106 farmed red deer (cervus elaphus) from the mazurian lake district in north-east poland were investigated for the presence of antibodies to neospora caninum. a modified neospora iscom-elisa was used for initial screening. all sera with optical density (od) values exceeding 0.400 absorbance units were further investigated by western blot analysis. eighteen sera were positive in both tests. six of these were from free living and 12 from farmed animals giving ...201021077439
monensin use against neospora caninum challenge in dairy cattle.using a randomized controlled trial design, a randomly allocated intervention group of 15 cows received a slow-release bolus that delivered 100 days of monensin. the negative control group of 15 cows received a placebo bolus that was identical to the monensin bolus, except without the monensin. two weeks after bolus administration, all cows were challenged with a 2 ml subcutaneous injection of a live tachyzoite suspension. whole blood and serum samples were collected from each cow every week for ...201021106293
neospora caninum seroprevalence in dairy and beef cattle from the northwest region of spain, galicia.herd and individual animal seroprevalence for neospora caninum (n. caninum) in dairy, beef and mixed cattle were obtained in all populations within the galician farmer sanitary defence associations (adsg) in 2004. all animals ≥1 year of age were examined serologically by indirect elisa. 1147 dairy herds (37,090 animals), 1464 beef herds (20,206 animals) and 141 mixed herds (2292 animals) were surveyed. true herd seroprevalence was estimated to be 80.6% (87.7% dairy, 76.7% beef and 78.4% mixed he ...201021145605
development of an indirect elisa-ncsrs2 for detection of neospora caninum antibodies in cattle.neosporosis is of alarming economic concern in the cattle industry. the effectiveness of diagnostic tests for detecting specific antibodies against neospora caninum is hampered by potential cross-reaction with other coccidia. use of a single specific antigen might improve test specificity. an indirect enzyme-linked immunosorbent assay (elisa) was developed using the truncated protein ncsrs2 expressed in escherichia coli. the elisa results were compared with those of the indirect fluorescence ant ...201021168278
tunicamycins, a class of nucleoside antibiotics similar to corynetoxins of the rathayibacter toxicus, increase susceptibility of mice to neospora caninum.neosporosis is the leading cause of abortion in cattle. neospora caninum-associated abortion may exhibit both endemic and epidemic patterns. it was reported that the epidemic outbreaks took place in the form of "abortion storms" and were not significantly correlated with seasonal changes or consumption of any particular feeds; and thus, the mechanisms by which the epidemic "abortion storms" are triggered remain unclear. annual ryegrass toxicity (argt) is a severe or fatal neurological disorder o ...201021168974
evidence of congenital transmission of neospora caninum in naturally infected water buffalo (bubalus bubalis) fetus from brazil.the aim of this study was to determine the congenital infection by neospora caninum in the water buffalo (bubalus bubalis), a natural intermediate host. nine pregnant water buffalos, raised under free-grazing condition, were slaughtered, and their fetuses were collected. samples of brain and thoracic fluid were obtained from those fetuses, with gestational ages ranging from 2 to 5 months. the dna of n. caninum was detected and identified in the brain of one of those fetuses, using two pcr assays ...201021181191
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