Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| study on the use of toltrazuril to eliminate neospora caninum in congenitally infected lambs born from experimentally infected ewes. | to determine if toltrazuril was effective in eliminating neospora caninum infection from congenitally infected lambs. twenty-eight ewes were allocated to 3 groups where animals in groups a and b were inoculated with 1 × 10(7)n. caninum tachyzoites on day 120 of gestation and group c was maintained as a negative control group. lambs born from ewes in group a were treated with toltrazuril (20mg/kg) on days 0, 7, 14 and 21 after birth. lambs in groups b and c were untreated. all lambs in groups a a ... | 2015 | 25935293 |
| in vitro effects of new artemisinin derivatives in neospora caninum-infected human fibroblasts. | from a panel of 34 artemisinin derivatives tested in vitro, artemisone, gc007 and gc012 were most efficacious at inhibiting neospora caninum replication (ic50 values of 3-54nm), did not notably impair the invasiveness of tachyzoites and were non-toxic for human foreskin fibroblasts (hffs). transmission electron microscopy of drug-treated n. caninum-infected hffs demonstrated severe alterations in the parasite cytoplasm, changes in the composition of the matrix of the parasitophorous vacuole (pv) ... | 2015 | 25934265 |
| isolation and molecular characterization of a new neospora caninum isolate from cattle in argentina. | neospora caninum is one of the most important causes of bovine abortion, but isolation of live parasites from infected tissue is difficult. the aims of the present study were to obtain new isolates of n. caninum from congenitally infected asymptomatic newborn cattle in argentina and to perform characterization by multilocus-microsatellite analysis. five clinically normal born calves, with demonstrable n. caninum antibodies in precolostrum serum by indirect fluorescent antibody test, were euthani ... | 2015 | 25913666 |
| antibodies to neospora caninum in sheep from slaughterhouses in the state of são paulo, brazil. | neosporosis is an emergent disease responsible for considerable economic impact due to reproductive losses. its zoonotic potential remains unknown. this study involved a survey of antibodies to neospora caninum in slaughtered sheep and their association with epidemiological variables. serum samples from 596 sheep from the states of são paulo and rio grande do sul, brazil, were collected in two slaughterhouses located in são paulo and evaluated by indirect fluorescence antibody test (ifat), using ... | 2015 | 25909261 |
| correction: ectopic expression of a neospora caninum kazal type inhibitor triggers developmental defects in toxoplasma and plasmodium. | 2015 | 25905860 | |
| the effect of a live neospora caninum tachyzoite vaccine in naturally infected pregnant dairy cows. | neosporosis, caused by the intracellular protozoan neospora caninum, is a major cause of abortion and reproductive failure in cattle worldwide. the principal route of transmission of neosporosis is via in utero infection of the offspring. there is no effective prophylactic treatment or vaccine available against bovine neosporosis. a n. caninum ncis491 isolate was examined for its ability to immunize and reduce abortions in naturally infected dairy cows under field conditions. n. caninum-seroposi ... | 2015 | 25890821 |
| influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis. | neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. in order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6) tachyzoites of the nc-spain7 isolate at days 40, 90 or 120 of gestation. infection during the first term resulted in the death of all foetuses between days 19 and 21 post-infection, showing mainly n ... | 2015 | 25884945 |
| comprehensive evaluation of toxoplasma gondii veg and neospora caninum liv genomes with tachyzoite stage transcriptome and proteome defines novel transcript features. | toxoplasma gondii is an important protozoan parasite that infects all warm-blooded animals and causes opportunistic infections in immuno-compromised humans. its closest relative, neospora caninum, is an important veterinary pathogen that causes spontaneous abortion in livestock. comparative genomics of these two closely related coccidians has been of particular interest to identify genes that contribute to varied host cell specificity and disease. here, we describe a manual evaluation of these g ... | 2015 | 25875305 |
| a large-scale proteogenomics study of apicomplexan pathogens-toxoplasma gondii and neospora caninum. | proteomics data can supplement genome annotation efforts, for example being used to confirm gene models or correct gene annotation errors. here, we present a large-scale proteogenomics study of two important apicomplexan pathogens: toxoplasma gondii and neospora caninum. we queried proteomics data against a panel of official and alternate gene models generated directly from rnaseq data, using several newly generated and some previously published ms datasets for this meta-analysis. we identified ... | 2015 | 25867681 |
| serological diagnosis of bovine neosporosis: a bayesian evaluation of two antibody elisa tests for in vivo diagnosis in purchased and abortion cattle. | currently, there are no perfect reference tests for the in vivo detection of neospora caninum infection. two commercial n caninum elisa tests are currently used in belgium for bovine sera (test a and test b). the goal of this study is to evaluate these tests used at their current cut-offs, with a no gold standard approach, for the test purpose of (1) demonstration of freedom of infection at purchase and (2) diagnosis in aborting cattle. sera of two study populations, abortion population (n=196) ... | 2015 | 25861822 |
| safety and immunogenicity of a soluble native neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in pregnant cattle. | the global economic impact of neospora caninum infection in cattle herds has promoted the development of vaccines that can be safely used during pregnancy. the aim of this study was to evaluate the safety and immunogenicity of a vaccine formulated with the soluble fraction of tachyzoite's lysate and a soy-based aqueous adjuvant (sncag/avec), which was protective in the mouse model and induced strong ifn-γ responses and high avidity antibodies in non-pregnant cattle. ten pregnant heifers were vac ... | 2015 | 25851060 |
| markers related to the diagnosis and to the risk of abortion in bovine neosporosis. | bovine neosporosis has emerged as a main cause of abortion in cattle worldwide. an important question to understand the disease is why not all infected cows abort. in the present review we summarize the knowledge on markers related to the diagnosis and more importantly to the risk of abortion in the infected cow. markers considered herein include those based on specific antibodies, antibody titers and antibody subtypes, cellular immunological markers, hormones and other proteins related to gesta ... | 2015 | 25841793 |
| a study of neospora caninum and toxoplasma gondii antibody seroprevalence in healthy cattle in the czech republic. | the aim of study was to test the sera of healthy dairy cows by elisas, the methods also used in other groups of animals in the czech republic, and thus to obtain actual data about n. caninum and t. gondii seroprevalence in cattle. | 2015 | 25780824 |
| neospora caninum recruits host cell structures to its parasitophorous vacuole and salvages lipids from organelles. | toxoplasma gondii and neospora caninum, which cause the diseases toxoplasmosis and neosporosis, respectively, are two closely related apicomplexan parasites. they have similar heteroxenous life cycles and conserved genomes and share many metabolic features. despite these similarities, t. gondii and n. caninum differ in their transmission strategies and zoonotic potential. comparative analyses of the two parasites are important to identify the unique biological features that underlie the basis of ... | 2015 | 25750213 |
| improving the gene structure annotation of the apicomplexan parasite neospora caninum fulfils a vital requirement towards an in silico-derived vaccine. | neospora caninum is an apicomplexan parasite which can cause abortion in cattle, instigating major economic burden. vaccination has been proposed as the most cost-effective control measure to alleviate this burden. consequently the overriding aspiration for n. caninum research is the identification and subsequent evaluation of vaccine candidates in animal models. to save time, cost and effort, it is now feasible to use an in silico approach for vaccine candidate prediction. precise protein seque ... | 2015 | 25747726 |
| occurrence of neospora caninum and toxoplasma gondii dna in brain tissue from hoary foxes (pseudalopex vetulus) in brazil. | the hoary fox (pseudalopex vetulus) is a wild canid native to brazil and is commonly found in the semiarid northeastern area living in contact with cattle. the main purpose of this study was to investigate the presence of neospora caninum and toxoplasma gondii dna in hoary foxes, in the state of paraíba, brazil. brain tissue samples were collected from 49 hoary foxes. from the samples, dna extraction and the polymerase chain reaction (pcr) were performed using specific primers for n. caninum and ... | 2015 | 25746974 |
| dynamic exophthalmos and lateral strabismus in a dog caused by masticatory muscle myositis. | a 5.5-year-old neutered male mixed-breed dog was presented for evaluation of a 5-month history of deviation of the right globe upon opening the mouth and a 1-month history of conjunctivitis in the same eye. clinical findings, diagnostic imaging results, treatment and follow-up are described. | 2015 | 25728848 |
| risk factors involved in transmission of toxoplasma gondii and neospora caninum infection in rabbit farms in northern italy. | in italy, rabbits are frequently reared for meat production. the aim of the study was to find the seroprevalence of t. gondii and n. caninum parasites, and risk factors of infection in rabbit farms. | 2015 | 26706976 |
| serological evidence of antibodies to neospora caninum in stray and owned grenadian dogs. | neospora caninum causes abortion in cattle and neuromuscular disease in dogs, world wide. cattle become infected by ingesting oocysts voided by dogs. the aim of this study was to estimate the seroprevalence of neospora caninum in two populations of dogs (stray and owned) in grenada, west indies. sera were collected from 625 dogs from all parishes in grenada. three hundred and sixty eight dogs were stray, while 257 dogs were owned. sera were tested for the presence of antibodies against n. caninu ... | 2015 | 26691257 |
| toxoplasma gondii and neospora caninum in dogs from the state of tocantins: serology and associated factors. | this study investigated occurrences of anti-neospora caninum and anti-toxoplasma gondii igg antibodies by means of the enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence assay (ifat), along with risk factors associated with toxoplasmosis and neosporosis, in 204 dogs from urban and rural areas of the municipality of araguaína, state of tocantins, brazil. one hundred and thirty samples (63.7%) were positive for t. gondii using elisa: 57.1% and 70.7% in the urban and rural ar ... | 2015 | 26689184 |
| risk factors for toxoplasma gondii and neospora caninum seropositivity in buffaloes in paraiba state, brazil. | the aims of this survey were to determine the frequency of anti-toxoplasma gondii and anti-neospora caninum antibodies and to identify the risk factors associated with seropositivity among buffaloes in the state of paraíba, brazil. this survey included 136 buffaloes belonging to 14 herds. to detect anti-t. gondii and anti-n. caninum antibodies, the indirect fluorescent antibody test (ifat) was used. among the 136 samples analyzed, 17 (12.5%) were positive for anti-t. gondii antibodies with titer ... | 2015 | 26689181 |
| toxoplasma gondii, neospora caninum and leishmania spp. serology and leishmania spp. pcr in dogs from pirassununga, sp. | we examined the presence of antibodies against the parasites toxoplasma gondii, neospora caninum, and leishmania spp., as well the presence of dna from leishmania spp., in dogs from pirassununga - sp. the seropositivity rate was compared with the animals' originating location. three hundred seventy-three blood samples from the county's kennel and local veterinary clinics were collected and analyzed. a total of 300 samples were tested for t. gondii and n. caninum using an indirect immunofluoresce ... | 2015 | 26689180 |
| [prevalence of neospora caninum infection in the intestine of pet dogs in some areas of anhui and zhejiang]. | to investigate the prevalence of neospora caninum infection in the intestine of pet dogs in areas of anhui and zhejiang. | 2015 | 26672220 |
| cell mediated immune responses in the placenta following challenge of vaccinated pregnant heifers with neospora caninum. | the aim of the present study was to investigate and correlate the cell-mediated immune response and pathological changes at the maternal-fetal interface of neospora-challenged pregnant cattle previously immunized with live and inactivated experimental vaccines. pregnant heifers naïve to neospora caninum were divided in 5 groups of 4 animals, each one immunized before mating: group a heifers were intravenously (iv) immunized with 6.25 × 10(7) live tachyzoites of the nc-6 strain; group b heifers w ... | 2015 | 26553499 |
| role of the chemokine receptor ccr5-dependent host defense system in neospora caninum infections. | neospora caninum, a toxoplasma gondii-like obligate intracellular parasite, causes abortion in cattle and neurological signs in canines. to understand neosporosis better, studies on host cell migration and host immune responses during the early phase of infection are important. although the c-c chemokine receptor 5 (ccr5) plays a crucial role in immune cell migration, the role played by it in protective immunity against n. caninum is poorly understood. | 2015 | 25558986 |
| serological survey for antibodies against bovine viral diarrhoea virus and neospora caninum in a population of south australian alpacas (vicugna pacos). | bovine viral diarrhoea virus (bvdv) and neospora caninum may cause clinical disease in alpacas. both diseases are present in the australian cattle population. the objective of this study was to perform a serological prevalence survey for bvdv and n. caninum exposure in a regional alpaca population of south australia. | 2015 | 26456156 |
| predominant role of interferon-γ in the host protective effect of cd8(+) t cells against neospora caninum infection. | it is well established that cd8(+) t cells play an important role in protective immunity against protozoan infections. however, their role in the course of neospora caninum infection has not been fully elucidated. here we report that cd8-deficient mice infected with n. caninum presented higher parasitic loads in the brain and lungs and lower spleen and brain immunity-related gtpases than their wild-type counterparts. moreover, adoptive transfer of splenic cd8(+) t cells sorted from n. caninum-pr ... | 2015 | 26449650 |
| brazilian donkeys (equus asinus) have a low exposure to neospora spp. | donkeys (equus asinus) are closely related to horses and are known to be infected by several equine pathogens. neospora caninum and neospora hughesi are protozoan parasites that infect horses, but they were not confirmed in donkeys up to this date. the aim of this study was to evaluate the exposure of donkeys (equus asinus) to neospora spp. using tachyzoites of n. caninum as antigen and employing two common serologic methods, ifat and immunoblot. sera from 500 donkeys were obtained from 30 munic ... | 2015 | 26444065 |
| a live vaccine against neospora caninum abortions in cattle. | 2015 | 25659274 | |
| vertical transmission in experimentally infected sheep despite previous inoculation with neospora caninum ncnz1 isolate. | recent reports indicate n. caninum has a possible role in causing abortions in sheep in new zealand. knowledge about the mode of transmission of neosporosis in sheep in new zealand is limited. this study aimed to determine the rate of vertical transmission that would occur in lambs born from experimentally inoculated ewes and to determine if previous inoculation would protect the lambs from n. caninum infection. a group of 50 ewes was divided into 2 groups with one group being inoculated with 5× ... | 2015 | 25638717 |
| seroprevalence and risk factors of toxoplasma gondii and neospora caninum infections in small ruminants in china. | little information is available about the seroprevalence of toxoplasma gondii and neospora caninum infections in sheep and goats in china. in the present investigation, the seroprevalence of t. gondii and n. caninum infections in sheep and goats were investigated in qinghai province, china between january 2012 and june 2013. a total of 1250 serum samples (600 sheep and 650 goats) collected from 8 administrative regions of qinghai province, china were evaluated by enzyme-linked immunosorbent assa ... | 2015 | 25591976 |
| immune response in the adipose tissue of lean mice infected with the protozoan parasite neospora caninum. | the adipose tissue can make important contributions to immune function. nevertheless, only a limited number of reports have investigated in lean hosts the immune response elicited in this tissue upon infection. previous studies suggested that the intracellular protozoan neospora caninum might affect adipose tissue physiology. therefore, we investigated in mice challenged with this protozoan if immune cell populations within adipose tissue of different anatomical locations could be differently af ... | 2015 | 25581844 |
| a vaccine formulation combining rhoptry proteins ncrop40 and ncrop2 improves pup survival in a pregnant mouse model of neosporosis. | currently there are no effective vaccines for the control of bovine neosporosis. during the last years several subunit vaccines based on immunodominant antigens and other proteins involved in adhesion, invasion and intracellular proliferation of neospora caninum have been evaluated as targets for vaccine development in experimental mouse infection models. among them, the rhoptry antigen ncrop2 and the immunodominant ncgra7 protein have been assessed with varying results. recent studies have show ... | 2015 | 25579396 |
| [endemic and epidemic bovine neosporosis: description of two events in beef cattle]. | the aim of this study was to describe two events in which neospora caninum was involved in bovine abortions in beef cattle. in the first event, 11 abortions in 57 heifers were recorded in 45 days. one aborted heifer was 5 times more likely to be seropositive than a non-aborted heifer (or=4.9; ic 1.2-19.9) (p<0.05). in the second event, no association between serological results and abortions were observed (or=0,69; 0,06-7,31) (p>0.05). neither antibodies nor isolation of other pathogens were ach ... | 2015 | 25576415 |
| investigation of neospora caninum seroprevalence and potential impact on reproductive success in semi-free-ranging père david's deer (elaphurus davidianus). | this study aimed to determine the prevalence and reproductive impact of neospora caninum in a herd of semi-free-ranging père david's deer (elaphurus davidianus) at a conservation facility, following a suspected abortion storm in 2008. serum samples (n=103) collected from 60 individuals between 2002 and 2011 were evaluated using competitive elisa for the detection of n. caninum-specific antibodies. of the 103 samples, 73 (70.9 per cent) were positive for n. caninum antibodies, with a mean±sd comp ... | 2015 | 26392906 |
| detection of neospora caninum-dna in brain tissues from pigeons in changchun, jilin (china). | neospora caninum is an intracellular protozoan infecting many domestic and wild animals. the domestic chicken (gallus domesticus) and the sparrow (passer domesticus) are known as natural intermediate hosts of n. caninum, whereas the role of other birds such as pigeons is still unclear. in the present study, pigeon brain tissues collected in jilin of china were screened by n. caninum specific-nested pcr to determine whether pigeons functioned as the natural intermediate hosts of n. caninum. the p ... | 2015 | 26385650 |
| neospora caninum in crows from israel. | a cross-sectional neospora caninum seroprevalence study was performed on free ranging crows (corvus cornix, corvus monedula and corvus splendens) from israel in order to assess their exposure to this pathogen and evaluate their role as potential hosts or as sentinels of infection. using the modified agglutination test (mat) with a cutoff titer of 1:100, 30 out of 183 crows (16.4%) were found to be n. caninum seropositive. positive results were validated and confirmed by the indirect fluorescent ... | 2015 | 26359640 |
| systemic neosporosis in a dog treated for immune-mediated thrombocytopenia and hemolytic anemia. | a 4-year-old male toy poodle was presented to the small animal veterinary hospital of the faculty of veterinary medicine of the autonomous university of mexico (fmvz, unam) because of depression, lethargy, and hemorrhages involving several areas of the skin and around the eyes. hematology data and a bone marrow analysis suggested hemolytic anemia and immune-mediated thrombocytopenia. the dog was treated with prednisone, and after one month the hematology variables improved. however, the dog's cl ... | 2015 | 26345698 |
| pathology in practice. meningoencephalitis and cerebellitis due to neospora caninum. | 2015 | 26331418 | |
| neospora caninum tachyzoite immunome study reveals differences among three biologically different isolates. | pathogenesis of bovine neosporosis is determined by different host- and parasite-dependent factors, including isolate virulence. a previous study identified that several neospora caninum tachyzoite proteins were more abundant in virulent isolates, nc-liv and nc-spain7, compared with the low-virulent isolate nc-spain1h. herein, we explored differences in the immunomes of these three isolates. protein extracts from the nc-liv, nc-spain1h and nc-spain7 isolates were analysed in a 3×3 design by 2-de ... | 2015 | 26324244 |
| three-dimensional coherent x-ray diffractive imaging of whole frozen-hydrated cells. | a structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of x-rays, has been limited by radiation damage. by alleviating this limitation, cryogenic coherent diffractive imaging (cryo-cdi) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. here, the first experimental demonstration of cryo-cdi for quantitative three-dimensional imaging of whole froz ... | 2015 | 26306199 |
| a review of neospora caninum in water buffalo (bubalus bubalis). | a number of countries in the world have reported infections with neospora caninum in water buffalo (bubalus bubalis), from africa to asia, europe and south america and recently australia. in general, clinical manifestations (such as abortion) seem rare, which has raised the prospect that buffalo may be inherently resistant to clinical effects of n. caninum infection. worldwide, the seroprevalence of n. caninum infection (as a measure of exposure determined by the detection of antibody) in buffal ... | 2015 | 26298507 |
| activity of several kinds of drugs against neospora caninum. | neosporosis caused by neospora caninum is a serious disease in cattle and dogs worldwide. it is the major cause of abortion and neonatal mortality in cattle. in this study, we evaluated the anti-n. caninum activity of chinese medicine extracts (curcumin, artemether), herbicides (atrazine, glyphosate), anticoccidiosis drugs (toltrazuril and ponazuril), cyclophosphamide, diminazene aceturate and praziquantel in vitro using parasite growth, replication and host cell invasion assays in human foreski ... | 2015 | 26264260 |
| seroprevalence and risk factors associated with exposure of water buffalo (bubalus bubalis) to neospora caninum in northeast thailand. | water buffalo are important draft animals for agriculture in resource-restricted areas worldwide. water buffalo were shown to be experimentally susceptible to infection with neospora caninum, potentially affected by neosporosis, and naturally exposed to the parasite in asia. although enzootic to thailand, the distribution of n. caninum among thai water buffalo is unclear. the objectives of this study were to determine the seroprevalence of n. caninum among water buffalo of northeast thailand and ... | 2015 | 25499824 |
| effects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with neospora caninum. | neospora caninum is an intracellular coccidian parasite causing abortion worldwide in dairy cattle. studies have shown that n caninum infection modifies endocrine patterns and that beef cows or cows with a crossbreed pregnancy, especially for a greater maternal-paternal genetic distance, show a reduced risk of abortion when naturally infected compared with pure-breed holstein friesian (hf) pregnancies. this study examined the effects of crossbreeding on plasma progesterone (p4), pregnancy-associ ... | 2015 | 25459029 |
| a 78 kda host cell invasion protein of neospora caninum as a potential vaccine candidate. | neosporosis is an intracellular protozoan disease caused by neospora caninum. until now, there is no effective vaccine to prevent neosporosis. the host cell binding protein has the potential as neosporosis vaccine. in the present study, a t7 phage display library was constructed and screened using vero cells to obtain host cell binding protein of n. caninum. two host cell binding proteins, a hypothetical protein of 78 kda (named as ncp78) homologous to the acylglycerol lipase of toxoplasma gondi ... | 2015 | 25448355 |
| blocking elisa using recombinant ncsrs2 protein for diagnosing bovine neosporosis. | neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. the reference method for serodiagnosis of neosporosis is the indirect fluorescent antibody test (ifat). however, ifat is laborious, expensive, and is not practicable in high throughput screening. in order to facilitate the serological diagnosis of neosporosis, we developed a blocking enzyme-linked immunosorbent assay (b-elisa) based on ncsrs2 recombinant protein (rncsrs2) and polyclonal an ... | 2015 | 25432863 |
| erratum to: neospora caninum causes severe economic losses in cattle in the humid pampa region of argentina. | 2015 | 25547804 | |
| abortion and foetal lesions induced by neospora caninum in experimentally infected water buffalos (bubalus bubalis). | the water buffalo (bubalus bubalis) is an important species in several countries for its milk and meat production, as well as for transport and other agricultural activities. it is, in general, considered more resistant than cattle to different parasitic diseases, also less demanding for forage quality. it has been postulated that buffalo may be resistant to abortion caused by neosporosis, because of high serological prevalences found in buffalo herds from different localities, with no descripti ... | 2015 | 25324135 |
| bombyx mori nucleopolyhedrovirus displaying neospora caninum antigens as a vaccine candidate against n. caninum infection in mice. | baculovirus display systems have been utilized for cell-specific gene transfer, regenerative medicine, and as vaccine vectors. in particular, baculovirus particles displaying surface antigens have been used as vaccines against some parasites and viruses. in this study, bombyx mori nucleopolyhedrovirus (bmnpv) particles displaying neospora caninum antigens (ncsag1, ncsrs2, and ncmic3) purified from the hemolymph or fat body of silkworm larvae were prepared to vaccinate mice against n. caninum. ea ... | 2015 | 25307182 |
| occurrence of mycobacterium avium subspecies paratuberculosis and neospora caninum in alberta cow-calf operations. | mycobacterium avium subsp. paratuberculosis (map) and neospora caninum (nc) are two pathogens causing important production limiting diseases in the cattle industry. significant impacts of map and nc have been reported on dairy cattle herds, but little is known about the importance, risk factors and transmission patterns in western canadian cow-calf herds. in this cross-sectional study, the prevalence of map and nc infection in southwest alberta cow-calf herds was estimated, risk factors for nc w ... | 2014 | 25277685 |
| inflammatory infiltration into placentas of neospora caninum challenged cattle correlates with clinical outcome of pregnancy. | infection with neospora caninum stimulates host cell-mediated immune responses, which may be responsible for placental damage leading to bovine abortion. the aim of this study was to compare immune responses in the bovine placenta, following experimental infection in different stages of pregnancy. placentomes were examined by immunohistochemistry and inflammation in early gestation was generally moderate to severe, particularly in the placentas carrying non-viable foetuses, whereas it was milder ... | 2014 | 24484200 |
| neospora caninum infection during early pregnancy in cattle: how the isolate influences infection dynamics, clinical outcome and peripheral and local immune responses. | this work studies the influence of neospora caninum intra-species diversity on abortion outcome, infection dynamics in terms of parasite dissemination and peripheral-local immune responses in pregnant cattle. animals were intravenously inoculated at day 70 of pregnancy with 10⁷ tachyzoites of two isolates showing marked differences in virulence in vitro and in pregnant mouse models: nc-spain7, a high virulence isolate, and nc-spain8, a low-to-moderate virulence isolate. after inoculation, pregna ... | 2014 | 24479988 |
| toxoplasma gondii infection in llama (llama glama): acute visceral disseminated lesions, diagnosis, and development of tissue cysts. | clinical toxoplasmosis has been reported in many species of warm-blooded animals but is rare in camelids. here we report acute fatal systemic toxoplasmosis involving heart, thyroid gland, stomach, intestine, diaphragm, kidneys, adrenal glands, and liver of a 13-mo-old llama (llama glama). many toxoplasma gondii tachyzoites were associated with tissue necrosis in multiple organs. death was attributed to severe myocarditis. ulcers associated with numerous tachyzoites were present in the c3 compart ... | 2014 | 24475973 |
| assessment of experimental infection for dogs using gallus gallus chorioallantoic membranes inoculated with neospora caninum. | the aim of this study was to evaluate parasitism kinetics and tissue lesions in the first week of infection by neospora caninum in dogs fed gallus gallus chorioallantoic membranes (cms) previously infected in ovo. five two-month-old pups were used. each dog was given five cms that were previously infected with n. caninum via the oral route. four animals were euthanized in the first week of infection. all four dogs had their stools examined one week prior to and up to the day they were euthanized ... | 2014 | 24473883 |
| gamma interferon production and plasma concentrations of pregnancy-associated glycoproteins 1 and 2 in gestating dairy cows naturally infected with neospora caninum. | gamma interferon (ifn-γ) production and cross-breed pregnancy have been attributed a role in protecting dairy cows infected with neospora caninum against abortion. plasma levels of pregnancy-associated glycoproteins-1 (pag-1) are a marker of placental/foetal well-being and of pag-2 is an abortion risk indicator in chronically n. caninum-infected animals. the present study examines, in cross-breed pregnancies, interactions between ifn-γ production and levels of pag-1 and pag-2 in non-aborting nat ... | 2014 | 24456132 |
| a transgenic neospora caninum strain based on mutations of the dihydrofolate reductase-thymidylate synthase gene. | neospora caninum is an apicomplexa parasite related to abortion and losses of fertility in cattle. the amenability of toxoplasma gondii and plasmodium to genetic manipulation offers several tools to determine the invasion and replication processes, which support posterior strategies related to the combat of these diseases. for plasmodium the use of pyrimethamine as an auxiliary drug on malaria treatment has been affected by the rise of resistant strains and the analyses on dihydrofolate reductas ... | 2014 | 24440296 |
| role of ifn-γ and lps on neuron/glial co-cultures infected by neospora caninum. | neospora caninum causes cattle abortion and neurological symptoms in dogs. although infection is usually asymptomatic, classical neurological symptoms of neosporosis may be associated with encephalitis. this parasite can grow in brain endothelial cells without markedly damages, but it can modulate the cellular environment to promote its survival in the brain. in previous studies, we described that ifn-γ decreased the parasite proliferation and down regulated nitric oxide (no) production in astro ... | 2014 | 25386119 |
| low prevalence of neospora caninum and toxoplasma gondii antibodies in dogs in jilin, henan and anhui provinces of the people's republic of china. | neospora caninum and toxoplasma gondii are important pathogens of worldwide distribution. n. caninum is a major cause of abortion in cattle and dogs are main reservoirs because they excrete the environmentally resistant oocysts. toxoplasmosis is a worldwide zoonosis and dogs are considered as sentinels for this parasite because of their close contact with people and cats; additionally dog meat is also used for human consumption in china. the aim of the present study was to assess the prevalence ... | 2014 | 25495616 |
| histological and immunohistochemical characterization of the inflammatory and glial cells in the central nervous system of goat fetuses and adult male goats naturally infected with neospora caninum. | neospora caninum is an apicomplexan protozoan that is considered one of the main agents responsible for abortion in ruminants. the lesions found in the central nervous system (cns) of aborted fetuses show multifocal necrosis, gliosis, and perivascular cuffs of mononuclear cells, but the inflammatory and glial cells have not been immunophenotypically characterized. the lesions in the cns of infected adult animals have rarely been described. therefore, in this study, we characterized the lesions, ... | 2014 | 25495444 |
| susceptibility to experimental infection of the invertebrate locusts (schistocerca gregaria) with the apicomplexan parasite neospora caninum. | neuropathogenesis is a feature of neospora caninum infection. in order to explore this in the absence of acquired host immunity to the parasite, we have tested infection in locusts (schistocerca gregaria). we show for the first time that locusts are permissive to intra-hemocoel infection with n. caninum tachyzoites. this was characterized by alteration in body weight, fecal output, hemoparasitemia, and sickness-related behavior. infected locusts exhibited progressive signs of sickness leading to ... | 2014 | 25493211 |
| gallus gallus domesticus are resistant to infection with neospora caninum tachyzoites of the nc-1 strain. | the aim of this study was to experimentally evaluate infection in gallus gallus domesticus with neospora caninum tachyzoites of the nc-1 strain. experimental infection was conducted in 90-day-old chickens, embryonated eggs and bioassays in dogs. in the first experiment, poults were randomly divided into four groups. groups i and ii were provided feed with coccidiostat, whereas groups iii and iv received feed without coccidiostat. when the poults from groups i and iii reached 90 days of age, they ... | 2014 | 25468016 |
| detection of antibodies to neospora caninum in moose (alces alces): the first report in europe. | neospora caninum dubey, carpenter, speer, topper et uggla, 1988 is a protozoan parasite originally reported as a major cause of bovine abortions worldwide. it is documented that the parasite is widely spread among non-carnivorous cervids. the purpose of this study was to investigate the seroprevalence of n. caninum in moose (alces alces linnaeus). blood samples collected in 2010 and 2012 in the northeastern poland were tested for antibodies to n. caninum by agglutination test (nat), a commercial ... | 2014 | 24684051 |
| neospora caninum calcium-dependent protein kinase 1 is an effective drug target for neosporosis therapy. | despite the enormous economic importance of neospora caninum related veterinary diseases, the number of effective therapeutic agents is relatively small. development of new therapeutic strategies to combat the economic impact of neosporosis remains an important scientific endeavor. this study demonstrates molecular, structural and phenotypic evidence that n. caninum calcium-dependent protein kinase 1 (nccdpk1) is a promising molecular target for neosporosis drug development. recombinant nccdpk1 ... | 2014 | 24681759 |
| control options for neospora caninum--is there anything new or are we going backwards? | recent work has highlighted and enumerated the economic annual losses due to neospora caninum abortions worldwide, which should provide strong motivation for the control of bovine neosporosis. however, with the recent withdrawal from sale of the only commercially available vaccine, control options for n. caninum have become more restricted. while researchers continue to work on developing alternative efficacious vaccines, what are the control options presently available for the cattle industries ... | 2014 | 24667014 |
| vaccines against neosporosis: what can we learn from the past studies? | neospora caninum is an intracellular apicomplexan parasite, which is a leading cause of abortion in cattle; thus neosporosis represents an important veterinary health problem and is of high economic significance. the parasite can infect cattle via trans-placental transmission from an infected cow to its fetus (vertical transmission), or through the oral route via ingestion of food or water contaminated with oocysts that were previously shed with the feces of a canid definitive host (horizontal t ... | 2014 | 24602874 |
| seroprevalences of toxoplasma gondii and neospora caninum in pet rabbits in japan. | the potential contamination of toxoplasma gondii and neospora caninum oocysts in the human environment is a concern from the public health viewpoint. however, estimation of their seroprevalences in humans cannot be performed in a manner that distinguishes between oocysts and tissue cysts as a source of infection. rabbits are considered popular pet animals in japan that can acquire natural infections by the aforementioned parasites only through the ingestion of oocysts. therefore, this study was ... | 2014 | 24584081 |
| adaptation of a commercial elisa to determine the igg avidity in sheep experimentally and naturally infected with neospora caninum. | recent reports indicate neospora caninum has a possible role in causing abortions in sheep in new zealand. knowledge about the epidemiology of neosporosis in sheep is limited. this study aimed to adapt and validate a commercially available elisa assay as an igg avidity assay to discriminate between acute (primary and re-inoculated) and chronic n. caninum infections in sheep. in addition, it was used to compare the antibody avidity values between lambs from ewes inoculated with n. caninum either ... | 2014 | 24582279 |
| comparative study for the detection of antibodies to neospora caninum in milk and sera in dairy cattle in southern romania. | the study aimed to assess the within-herd neospora caninum exposure in dairy cattle in southern romania, based on the detection of specific antibodies in milk and serum. a total of 104 paired samples of milk and serum were collected from four dairy farms. individual samples were analyzed for n. caninum antibodies by elisa: idexx neospora ab (idx) (three farms: a, b, c; n = 60) and id-vet lab (idv) (farm d; n = 44). additionally, four pooled milk samples, one per each farm (a, b, c) and a compose ... | 2014 | 24570044 |
| pcr detection of neospora caninum in water buffalo foetal tissues. | the seroprevalence of neospora caninum was surveyed by an elisa kit on two water buffalo herds of southern italy. seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. tissue samples collected from three aborted fetuses from the same herds were investigated for n. caninum presence by pcr assays targeting the 18s and the nc5 dna sequences, respectiv ... | 2014 | 24570043 |
| pathogenicity of nc-bahia and nc-1 strains of neospora caninum in experimentally infected cows and buffaloes in early pregnancy. | neospora caninum is a protozoan parasite known as an important cause of bovine abortion worldwide. little is currently known about how different strains of n. caninum vary in their pathogenicity. in this study, we compared a brazilian strain, nc-bahia, with the first isolate of this coccidian, nc-1. eight cows and seven buffaloes were submitted to fixed-time artificial insemination protocols for a better control of pregnancy. group 1 was inoculated with nc-bahia (n = 8; five cows and three buffa ... | 2014 | 24562816 |
| transmission paths of neospora caninum in a dairy herd of crossbred cattle in the northeast of brazil. | the aim of this study was to determine the transmission paths of neospora caninum in a dairy herd of crossbred cattle. two hundred and ninety animals were grouped according to the year of their birth to verify the distribution of infection according to age. the blood of 196 cattle was collected thrice, with an average interval of 210 days, to evaluate the existence of horizontal transmission in the herd. to evaluate vertical transmission, the blood of 64 calves was collected prior to ingestion o ... | 2014 | 24560936 |
| infection by toxoplasma gondii specifically induces host c-myc and the genes this pivotal transcription factor regulates. | toxoplasma gondii infection has previously been described to cause dramatic changes in the host transcriptome by manipulating key regulators, including stats, nf-κb, and micrornas. here, we report that toxoplasma tachyzoites also mediate rapid and sustained induction of another pivotal regulator of host cell transcription, c-myc. this induction is seen in cells infected with all three canonical types of toxoplasma but not the closely related apicomplexan parasite neospora caninum. coinfection of ... | 2014 | 24532536 |
| characterization of immune cell infiltration in the placentome of water buffaloes (bubalus bubalis) infected with neospora caninum during pregnancy. | neospora caninum infection in cattle stimulates host immune responses, which may be responsible for placental damage leading to abortion. susceptibility of water buffaloes (bubalus bubalis) to neosporosis is not well understood, although vertical transmission and fetal death have been documented. the aim of this study was to characterize the immune response in the placentome of water buffalo following experimental infection in early gestation with the nc-1 strain of n. caninum. placentomes were ... | 2014 | 24529512 |
| isolation of viable neospora caninum from brains of wild gray wolves (canis lupus). | neospora caninum is a common cause of abortion in cattle worldwide. canids, including the dog and the dingo (canis familiaris), the coyote (canis latrans), and the gray wolf (canis lupus) are its definitive hosts that can excrete environmentally resistant oocysts in the environment, but also can act as intermediate hosts, harboring tissue stages of the parasite. in an attempt to isolate viable n. caninum from tissues of naturally infected wolves, brain and heart tissue from 109 wolves from minne ... | 2014 | 24522164 |
| reproductive implications of exposure to toxoplasma gondii and neospora caninum in western grey kangaroos (macropus fuliginosus ocydromus). | australian marsupials are thought to be particularly vulnerable to pathologic impacts of toxoplasma gondii, and they may be similarly affected by neospora caninum. pathology due to either organism could be expressed as reduced female reproductive performance. we studied adult female western grey kangaroos (macropus fuliginosus ocydromus) from suburban perth, western australia, between may 2006 and october 2008. we used indirect fluorescent antibody tests to look for evidence of exposure to t. go ... | 2014 | 24506426 |
| differential induction of tlr3-dependent innate immune signaling by closely related parasite species. | the closely related protozoan parasites toxoplasma gondii and neospora caninum display similar life cycles, subcellular ultrastructure, invasion mechanisms, metabolic pathways, and genome organization, but differ in their host range and disease pathogenesis. type ii (γ) interferon has long been known to be the major mediator of innate and adaptive immunity to toxoplasma infection, but genome-wide expression profiling of infected host cells indicates that neospora is a potent activator of the typ ... | 2014 | 24505488 |
| a survey of toxoplasma gondii and neospora caninum infecting house mice from a hybrid zone. | toxoplasma gondii and neospora caninum are closely related coccidian parasites infecting a wide range of wild and domestic animals as intermediate hosts, and rodents serve as important reservoir hosts during the life cycles of these parasites. the present study is aimed at identifying t. gondii and n. caninum infection in 360 wild house mice (mus musculus) collected across the czech-german border, where 2 genetically distinct mouse subspecies meet and hybridize. toxoplasma gondii or n. caninum d ... | 2014 | 23927367 |
| comparison of different serological methods to detect antibodies specific to neospora caninum in bovine and canine sera. | neospora caninum is an apicomplexan parasite responsible for paresis in dogs and abortion in cattle worldwide. dogs serve as a definitive host, while cattle serve as intermediate host. many different methods have been developed to detect specific antibodies present in cattle and dog serum. in the present study, the dense granule protein ncgra6 was incorporated in a latex beads agglutination test (lat), and compared to other serological methods, including enzyme-linked immunosorbent assay, the di ... | 2014 | 24398907 |
| diagnostic potential of anti-rncp-43 polyclonal antibodies for the detection of neospora caninum. | neosporosis is a disease caused by the apicomplexan parasite neospora caninum, which is closely related to toxoplasma gondii. n. caninum infection represents an important cause of reproductive failure in sheep, goats, horses, and cattle worldwide. the diagnosis of neosporosis is based on the detection of pathogen-specific antibodies in animal sera or the presence of tissue cysts. however, morphological similarities and serological cross-reactivity between n. caninum and t. gondii can result in t ... | 2014 | 24310934 |
| neospora caninum is a cause of perinatal mortality in axis deer (axis axis). | neospora caninum is a worldwide distributed protozoan that may cause neuromuscular disease in dogs and reproductive failure in domestic and wild ruminants. one axis fawn (axis axis) and four neonates from the same deer herd died at a zoo in argentina within a four-month period. the fawn presented with dilatation of the anal sphincter at birth and incontinence, developed weakness and ataxia and died at 14 days of age. at necropsy, a mega formation of the distal large intestine was observed. micro ... | 2014 | 24295954 |
| neospora caninum and toxoplasma gondii: relationship between hepatic lesions, cytological and biochemical analysis of the cavitary liquid during the acute phase of the diseases in experimental models. | the objective of this study was to evaluate the pathogenesis of ascites in mice infected with toxoplasma gondii and gerbils infected with neospora caninum during the acute phase disease. for that, 12 gerbils [experiment i: not infected/control (n=6) and infected (n=6)] and 12 mice [experiment ii: control (n=6) and infected (n=6)] were used. infected gerbils and mice showed marked ascites on days 5-7 post-infection (pi), while the not-infected animals had not ascites. peritoneal liquid was collec ... | 2014 | 24275558 |
| neospora caninum dna detection by taqman real-time pcr assay in experimentally infected pregnant heifers. | neosporosis has been considered the main cause of abortion between the first and the second trimester of pregnancy in cattle. therefore, the objective of this study was to identify the presence of neospora caninum dna obtained from experimental models based on the evaluation of different areas of the fetal nervous system and organs from heifers previously inoculated with nc-1 after or before insemination. this study was performed with hereford × nelore (n=29) heifers and all animals were conside ... | 2014 | 24269161 |
| molecular identification of neospora caninum from calf/foetal brain tissue and among oocysts recovered from faeces of naturally infected dogs in southern ethiopia. | this study sought to confirm and investigate further recently published information regarding the occurrence of neospora caninum in cattle in ethiopia and investigate infection in dogs, the canine definitive host, in this region. faecal samples from 383 dogs in hawassa, ethiopia were examined by microscopy for neospora-like oocysts, and positive samples then analysed by a molecular approach (dna isolation, pcr and sequencing at the its1 gene). brain tissue samples from four late term aborted foe ... | 2014 | 24189135 |
| oxidative stress in brain tissue of gerbils experimentally infected with neospora caninum. | neospora caninum infection is generally latent and asymptomatic, and it results in the formation of dormant encysted bradyzoites that remain in the brain and other tissues of infected animals for life, causing major economic and pathological problems. the aim of this study was to assess the relation between infection by n. caninum and its damage to brain tissue through the evaluation of biomarkers of oxidative stress during the acute and chronic phases of the disease. sixteen gerbil (meriones un ... | 2014 | 24188248 |
| neospora caninum tachyzoites inoculated by the conjunctival route are not vertically transmitted in pregnant cattle: a descriptive study. | the aim of this study was to evaluate whether neospora caninum tachyzoites (nc-1) inoculated by the conjunctival route in pregnant cows were able to generate infection in their fetuses. group 1 contained 2 naturally infected cows; group 2 contained two cows inoculated intravenously with 2.5 × 10(8) tachyzoites, group 3 contained two cows inoculated with 2.5 × 10(8) tachyzoites by the conjunctival route, and group 4 contained two uninfected control cows. the four inoculated cows from groups 2 and ... | 2014 | 24184173 |
| clinical outcome and vertical transmission variability among canine neospora caninum isolates in a pregnant mouse model of infection. | we compared the clinical outcome and vertical transmission of six canine neospora caninum isolates using a pregnant balb/c model. four of the isolates were obtained from oocysts of naturally infected dogs (nc-ger2, nc-ger3, nc-ger6 and nc-6arg) and two were from diseased dogs with neurological signs (nc-bahia and nc-liv). the dams were inoculated with 2×106 tachyzoites of each isolate at day 7 of pregnancy. morbidity, mortality and the antibody responses were evaluated in both the dams and the o ... | 2014 | 24148606 |
| protective effect of intranasal immunization with neospora caninum membrane antigens against murine neosporosis established through the gastrointestinal tract. | neospora caninum is an apicomplexa parasite that in the last two decades was acknowledged as the main pathogenic agent responsible for economic losses in the cattle industry. in the present study, the effectiveness of intranasal immunization with n. caninum membrane antigens plus cpg adjuvant was assessed in a murine model of intragastrically established neosporosis. immunized mice presented a lower parasitic burden in the brain on infection with 5 × 10(7) tachyzoites, showing that significant p ... | 2014 | 24128071 |
| tracking neospora caninum parasites using chimera monoclonal antibodies against its surface antigen-related sequences (rncsrs2). | neosporosis, an infectious disease of cattle and dogs, causes an abortion in cattle, which has a major damage on the dairy industry worldwide. tracking of neospora caninum parasite that is responsible for neosporosis is required for the prevention of this infectious disease. we developed three chimera monoclonal antibodies consist of variable regions of murine antibody and constant regions of human antibody against n. caninum. recombinant surface antigen-related sequence 2 (rncsrs2) of n. caninu ... | 2014 | 24103572 |
| prevalence of immunoglobulin g (igg) antibody to neospora caninum in dairy cattle of hamedan province, west of iran. | bovine neosporosis caused by the apicomplexan protozoan parasite n. caninum, was initially recognized in 1989 and is now reported as a leading infectious cause of reproductive failure in dairy cattle in world wide. the aim of this study was to determine the seroprevalence of n. caninum infection in industrial dairy cattle of hamedan province (west of iran) by elisa method. blood samples were collected from 492 cattle in 41 farms. antibodies to n. caninum were found in 63(12.80%) sera. a signific ... | 2014 | 25568710 |
| seroprevalence of neospora caninum in slaughtered native cattle in kurdistan province, iran. | neospora caninum is a worldwide distributed pathogen which causes abortion in cattle leading to economic loss in the cattle industry. the aim of this study was to determine the seroprevalence of n. caninum antibodies in the native cattle slaughtered in various areas of kurdistan province (western iran) from september 2010 to september 2011. serum samples from 368 cattle slaughtered in seven slaughterhouses in this region were taken for detection of anti-n. caninum antibodies using commercial n. ... | 2014 | 25568696 |
| flavonoids modulate the proliferation of neospora caninum in glial cell primary cultures. | neospora caninum (apicomplexa; sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. in the central nervous system of dogs infected with n. caninum, cysts were detected that exhibited gliosis and meningitis. flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. in this study, we investigated the effects of flavonoids in a well-established i ... | 2014 | 25548412 |
| anti-neospora caninum antibody detection and vertical transmission rate in pregnant zebu beef cows (bos indicus): neospora caninum in pregnant beef cows (bos indicus). | the aim of the present study was to evaluate anti-neospora caninum antibodies and the vertical transmission rate in naturally infected pregnant zebu beef cows (bos indicus) reared on pasture. the present study began with 200 cows from four farms (50 cows from each farm), and these animals were submitted to timed artificial insemination (tai). after ultrasonography, 76 pregnant cows were selected, 22, 15, 22, and 17, respectively, from farms 1, 2, 3, and 4. blood samples were taken from cows thri ... | 2014 | 25193073 |
| toxoplasma gondii and neospora caninum infections in goat abortions from argentina. | the aims of this study were to identify the occurrence of toxoplasma gondii and neospora caninum abortions in goats from argentina by serological, macroscopical and microscopical examination and bioassay, and to characterize the obtained isolates by molecular techniques. for this purpose, 25 caprine fetal fluids, 18 caprine fetal brains and 10 caprine placentas from 8 dairy/meat goat farms from argentina were analyzed. gestational age of the aborted fetuses was determined in 18 cases. protozoal ... | 2014 | 25128663 |
| the chloramphenicol acetyltransferase vector as a tool for stable tagging of neospora caninum. | neospora caninum is an obligate intracellular apicomplexa, a phylum where one of the current methods for functional studies relies on molecular genetic tools. for toxoplasma gondii, the first method described, in 1993, was based on resistance against chloramphenicol. as in t. gondii, we developed a vector constituted of the chloramphenicol acetyltransferase gene (cat) flanked by the n. caninum dihydrofolate reductase-thymidylate synthase (dhfr-ts) 5' coding sequence flanking region. five weeks a ... | 2014 | 25127750 |
| fluorescent ester dye-based assays for the in vitro measurement of neospora caninum proliferation. | techniques for the measurement of parasite loads in different experimental models have evolved throughout the years. the quantification of stained slides using regular cytological stains is currently the most common technique. however, this modality of evaluation is labor-intensive, and the interpretation of the results is subjective because the successes of the assays mainly rely on the abilities of the professionals involved. moreover, the novel genetic manipulation techniques that are commonl ... | 2014 | 25095733 |
| cytokine expression in the placenta of pregnant cattle after inoculation with neospora caninum. | neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated th1 type response at the maternal-foetal interface. different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most commo ... | 2014 | 25091332 |
| occurrences of anti-toxoplasma gondii and anti-neospora caninum antibodies in barbary sheep at curitiba zoo, southern brazil. | barbary sheep (ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. blood samples from 17 barbary sheep at the curitiba zoo were collected to evaluate occurrences of anti-toxoplasma gondii and anti-neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (ifat). anti-t. gondii and anti-n. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. the present study has shown that ... | 2014 | 25054509 |
| comparison of host cell invasion and proliferation among neospora caninum isolates obtained from oocysts and from clinical cases of naturally infected dogs. | in a previous study we have shown that the in vitro invasion rate (ir) and tachyzoite yield (ty) are associated with the virulence phenotypes of neospora caninum isolates of bovine origin. in addition, we recently observed marked differences in virulence when canine isolates were compared in a pregnant balb/c mouse model. in this study, we investigated whether invasion and proliferation capacities could be used as virulence-related n. caninum phenotypic traits. of the isolates compared in mice, ... | 2014 | 25045851 |
| discovering a vaccine against neosporosis using computers: is it feasible? | a vaccine is urgently needed to prevent cattle neosporosis. this infectious disease is caused by the parasite neospora caninum, a complex biological system with multifaceted life cycles. an in silico vaccine discovery approach attempts to transform digital abstractions of this system into adequate knowledge to predict candidates. researchers need current information to implement such an approach, such as understanding evasion mechanisms of the immune system, type of immune response to elicit, av ... | 2014 | 25028089 |