Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| prevalence of antibodies to neospora caninum and toxoplasma gondii in swedish dogs. | 1994 | 7676930 | |
| sequence analysis and comparison of ribosomal dna from bovine neospora to similar coccidial parasites. | the nuclear small subunit ribosomal rna (nss-rrna) gene sequence of neospora spp. isolated from cattle was analyzed and compared to the sequences from several closely related cyst-forming coccidial parasites. double-stranded dna sequencing of 5 bovine neospora spp. isolates (bpa1-4), 2 neospora caninum isolates (nc-1 and nc-3), and 3 toxoplasma gondii isolates (rh, gt-1, ct-1) were performed and compared to each other, as well as to other sequences available in genbank for the nc-1 isolate, sarc ... | 1995 | 7623193 |
| neospora caninum infection in three dogs. | infection with neospora caninum in three young dogs is described. the predominant clinical signs were lower motor neuron deficits of the pelvic limbs, bladder and rectum. in two cases there was liver infection and dysfunction. the younger dogs had an acute onset rapidly progressive syndrome. the older dog had a similar but more chronic course. the diagnosis was confirmed by an immunofluorescence antibody test. the parasite is sensitive to clindamycin and trimethoprim/sulphonamide preparations, h ... | 1995 | 7603059 |
| an enzyme-linked immunosorbent assay (elisa) for serological diagnosis of neospora sp. infection in cattle. | a kinetic enzyme-linked immunosorbent assay (elisa) was developed and optimized for detection of antibodies to neospora sp. in cattle. sonicated tachyzoites of neospora sp. isolated from an aborted bovine fetus were used as antigen. variability in immunoblot patterns among positive sera, and the fact that all life stages of the parasites are unknown, justified use of a multiple-antigen elisa to allow for maximum sensitivity. immunoblot analysis revealed negligible cross-reactions between toxopla ... | 1995 | 7578451 |
| a survey of the incidence of neospora caninum infection in aborted and stillborn bovine fetuses in england and wales. | selected brains and fetal viscera from 190 aborted or stillborn bovine fetuses submitted to veterinary investigation centres in england and wales between august 1992 and january 1993 were examined histologically. non-suppurative inflammation of the brain and/or myocardium and placental cotyledons was identified by light microscopy in 20 (10.5 per cent). an immunocytochemical examination of fixed tissue sections using antisera against neospora caninum, toxoplasma gondii and sarcocystis species re ... | 1995 | 7571263 |
| neospora caninum infection in a litter of labrador retriever dogs in denmark. | 1995 | 7502956 | |
| genetic comparison of neospora caninum with toxoplasma and sarcocystis by random amplified polymorphic dna-polymerase chain reaction. | to determine the relationship of neospora caninum to protozoa classified in the family sarcocystidae of the phylum apicomplexa, the genomes of n. caninum, three toxoplasma gondii strains (rha, cep, tpr) and three sarcocystis species (s. tenella, s. muris, s. gigantea) that were thought to be closely related coccidia were compared by the random amplified polymorphic dna (rapd) polymerase chain reaction (pcr) technique. the genomic dnas were amplified by the use of seven 10-mer arbitrary sequence ... | 1995 | 7501633 |
| identification and characterization of neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. | the purpose of the present study was to identify antigens of the protozoan neospora caninum that could be useful for the diagnosis of neosporosis in domestic animals. as revealed by immunoblotting, immune sera from a wide range of animal species exhibited a similar recognition pattern of four major and several minor n. caninum antigens. in contrast to preinoculation sera, all tested immune sera recognized nonreduced immunodominant 17-, 29-, 30-, and 27-kda antigens. a 46-kda protein which showed ... | 1994 | 7496948 |
| interferon gamma inhibits the intracellular multiplication of neospora caninum, as shown by incorporation of 3h uracil. | an assay was developed to quantify the growth of two different isolates of the protozoon neospora caninum within ovine fibroblast cells in vitro by differential uptake of 3h uracil. the nc-1 isolate of n. caninum multiplied more quickly in culture than the nc liverpool isolate, as reflected by increased incorporation of isotope by the former over a shorter period of time. after the parasites had left the ruptured host cells, there was very little incorporation of isotope. this suggested that mul ... | 1995 | 7490344 |
| abortions, fetal death, and stillbirths in pregnant pygmy goats inoculated with tachyzoites of neospora caninum. | neospora caninum-induced abortion is a major production problem in the dairy cattle industry in the united states and worldwide. abortions attributable to naturally acquired n caninum infection also have been observed in pygmy goats. we studied experimentally induced infections with n caninum in pregnant pygmy does to determine whether abortions attributable to n caninum infection would occur after inoculation. seven pregnant pygmy does (1 control doe and 6 inoculated with n caninum) were studie ... | 1995 | 7486395 |
| experimental oral inoculations in birds to evaluate potential definitive hosts of neospora caninum. | experimental oral inoculations to evaluate potential definitive hosts of neospora caninum were conducted by feeding infected rodent tissues to 9 carnivorous birds of 4 species. birds included 2 red-tailed hawks (buteo jamaicensis), 2 turkey vultures (cathartes aura), 2 barn owls (tyto alba), and 3 american crows (corvus brachyrhynchus). the rodents (mice or rats) had been inoculated with 100,000 culture-derived tachyzoites of n. caninum 1-6 mo before feeding to the birds. fecal samples were coll ... | 1995 | 7472875 |
| vertical transmission of neospora caninum in mice. | herein we report a murine model to examine transplacental transmission, transmammary transmission, or both, of neospora caninum. prevalence of transplacental transmission in outbred swiss-webster mouse pups was 85%, with 11 of 13 litters containing at least 1 transplacentally infected pup. sixty-two percent of litters born to experimentally infected dams contained 85% or more transplacentally infected pups. numbers of pups congenitally infected per litter was higher if dams were inoculated durin ... | 1995 | 7472863 |
| newly recognized fatal protozoan disease of dogs. | histologic sections and case histories from 23 dogs with proven fatal toxoplasmosis-like illness at the angell memorial animal hospital were reviewed. toxoplasma gondii was identified in 13 dogs. a newly identified parasite, neospora caninum, structurally distinct from t gondii, was found in 10 dogs. the newly discovered organism, belonging to a new genus and new species, formed meronts in many tissues of the dogs, especially the brain and spinal cord. neospora caninum was located directly in th ... | 1988 | 3391851 |
| neonatal neospora caninum infection in dogs: isolation of the causative agent and experimental transmission. | neospora caninum infection was diagnosed in 5 young dogs from 2 litters with a common parentage. the pups were born healthy, but developed hind limb paresis 5 to 8 weeks after birth. the predominant lesions were polyradiculoneuritis and granulomatous polymyositis. neospora caninum was seen microscopically in sections of naturally infected pups, and was isolated in cell cultures, mice, and dogs inoculated with infected canine tissues. antibodies to n caninum were detected in sera of infected dogs ... | 1988 | 3144521 |
| ultrastructure of tachyzoites, bradyzoites and tissue cysts of neospora caninum. | dividing tachyzoites of neospora caninum were 4 x 3 microns and had ultrastructural characteristics typical for the cyst-forming coccidia. unusual ultrastructural characteristics of fully-formed tachyzoites included no micropores, 8-12 anterior and 4-6 posterior rhoptries, and a few posterior micronemes. most tachyzoites were located free in the host cell cytoplasm; only a few occurred within a parasitophorous vacuole. parasite multiplication appeared to be rapid because most organisms were in v ... | 1989 | 2810142 |
| transplacental neospora caninum infection in dogs. | a beagle bitch was inoculated sc and im with 1.5 million neospora caninum tachyzoites on the 35th day of gestation. eight pups were born alive 28 days after n caninum inoculation of the bitch. pup 1 did not breathe and died while still enclosed in fetal membranes. pup 2 died 2 days after birth (dab). pups 3, 4, and 5 were euthanatized on 2, 3, and 20 dab, respectively, because they were hypothermic and not nursing. pups 6, 7, and 8 remained clinically normal. indirect fluorescent n caninum serum ... | 1989 | 2802335 |
| neospora caninum (protozoa: apicomplexa) infections in mice. | groups of mice were given 0 mg, 4 mg, or 2 mg of methylprednisolone acetate (mpa) 7 days prior to, the day of, and 7 days after subcutaneous inoculation with 0 or 2 x 10(5) tachyzoites of neospora caninum. clinical signs of disease were seen only in mice given both mpa and n. caninum tachyzoites. mice given 4 mg mpa and n. caninum tachyzoites developed severe disseminated neosporosis and most died or were killed when comatose 11-13 days postinoculation (pi). acute pneumonia, polymyositis, enceph ... | 1989 | 2795380 |
| transplacental neospora caninum infection in cats. | transplacental transmission of neospora caninum was studied in 2 pregnant cats (queens). queen 1 was inoculated subcutaneously with 2 x 10(6) cell culture-derived n. caninum tachyzoites on day 47 of gestation. she gave birth to a full-term kitten on the 17th day after inoculation. the kitten died the second day after birth due to generalized n. caninum infection. the mother cat was killed on the third day after parturition and was found to have a macerated kitten in the uterus. severe placentiti ... | 1989 | 2795379 |
| encephalomyelitis and myositis in a boxer puppy due to a neospora-like infection. | a 10-week-old boxer dog from uppsala, sweden, was killed because of hind limb paralysis. the hind limb muscles were grossly atrophied and encephalomyelitis and myositis were the predominant microscopic lesions. neospora caninum-like tachyzoites were found in the brain and skeletal muscles, and tissue cysts were found in the brain. the organism was not toxoplasma gondii, as t. gondii antibody was not found in the indirect fluorescent antibody test in serum samples from the puppy and the dam, and ... | 1989 | 2773275 |
| the neuropathology in toxoplasmosis-like infection caused by a newly recognized cyst-forming sporozoon in dogs. | the pathomorphological changes in the central nervous system associated with an infection with a previously reported, toxoplasma-like, cyst-forming sporozoon were studied by means of light microscopy, including immunohistochemistry, and transmission electron microscopy in seven dogs, aged 4 months to 2 years. tachyzoites (endozoites) within parasitophorous vacuoles were found in neurons, astrocytes, macrophages, vascular pericytes and probably also in fibroblasts, while cyst stages were only obs ... | 1989 | 2730788 |
| immunohistochemical diagnosis of neospora caninum in tissue sections. | an avidin-biotin-peroxidase complex immunoperoxidase staining method was developed to detect neospora caninum in formalin-fixed, paraffin-embedded tissue sections. specific antiserum to n caninum was made in rabbits and used to probe tissues from dogs naturally and experimentally infected with n caninum. the test detected tachyzoites and bradyzoites of n caninum. a reaction was not observed to toxoplasma gondii, hammondia hammondi, sarcocystis cruzi, s capricanis, s tenella, besnoitia jellisoni, ... | 1989 | 2694869 |
| evaluation of anti-coccidial drugs' inhibition of neospora caninum development in cell cultures. | eight anti-coccidial drugs were examined for their efficacies in preventing development of neospora caninum in bovine monocyte cell cultures. lasalocid sodium (0.05 microgram/ml), monensin sodium (0.05 microgram/ml), piritrexim (0.01 microgram/ml), pyrimethamine (0.05 microgram/ml), and trimethoprim (5.0 micrograms/ml) were effective in preventing development of intracellular n. caninum tachyzoites (p less than 0.05). no differences (p greater than 0.05) in mean numbers of infected cells compare ... | 1989 | 2614609 |
| a neospora-like protozoon found in an aborted bovine placenta. | portions of the placenta and kidneys from a 5-month-old bovine fetus were examined histologically. the placental cotyledonary villi were necrotic and protozoa were found in lesions. the organisms were in trophoblasts, periodic acid schiff-negative and did not react in an immunoperoxidase test using anti-toxoplasma gondii serum. individual zoites were approximately 5 x 2 microns and the biggest collection was 45 x 35 microns. the organism in the present case was structurally distinct from sarcocy ... | 1989 | 2588466 |
| in vitro development of neospora caninum (protozoa: apicomplexa) from dogs. | the development of neospora caninum isolated from naturally infected dogs was examined in mammalian cell cultures. tachyzoites developed by endodyogeny when inoculated onto bovine monocyte or bovine cardiopulmonary artery endothelial cell cultures. tachyzoites were 5.0 by 2.0 microns and had a posteriorly located nucleus. cytopathogenic effects of parasite development consisted of the formation of holes in the cell monolayer associated with the rupture of infected host cells. serial passage of t ... | 1989 | 2493088 |
| fatal neospora caninum infection in kittens. | three 3-day-old kittens were inoculated subcutaneously and orally with neospora caninum tachyzoites. a littermate and the queen were not inoculated with n. caninum and served as controls. kitten 1 died between 14 and 16 days postinoculation (dpi) and was eaten by the mother. kitten 2 died 17 dpi and kitten 3 was euthanized 29 dpi in a moribund condition. the control littermate and the dam remained healthy. granulomatous skeletal myositis and nonsuppurative encephalomyelitis were the main lesions ... | 1989 | 2493087 |
| neospora caninum-like protozoon associated with fatal myelitis in newborn calves. | neospora caninum-like organisms were found in histologic sections of spinal cord of 4 paralyzed calves as reported by parish et al. (1987). tachyzoites divided by endodyogeny. tissue cysts were up to 62 microns wide and the cyst wall was up to 2.5 microns thick. the organism in calves was structurally distinct from toxoplasma gondii and sarcocystis species and reacted positively with anti-n. caninum serum in an immunoperoxidase test. | 1989 | 2493086 |
| neosporosis-like abortions in a herd of dairy cattle. | twenty-nine cows from a 240-cow drylot holstein dairy in north-central new mexico aborted over a period of approximately 5 months. nine aborted fetuses were necropsied, and all but 1 of the fetuses were between 5 and 7 months of gestation. microscopic examination of fetal tissues revealed focal necrotizing encephalitis and nonsuppurative myocarditis in 7 of the 9 fetuses. additional lesions observed in some fetuses were focal hepatic necrosis, nonsuppurative myositis, focal necrotizing placentit ... | 1989 | 2488345 |
| neosporosis in dogs. | a bitch was inoculated subcutaneously and intramuscularly with neospora caninum tachyzoites on day 35 of pregnancy. eight pups were born 28 days later. five pups became ill and necropsies were performed before 20 days of age. three pups and the bitch remained clinically normal for 7 weeks after parturition when they were intramuscularly injected with 40 mg kg-1 methylprednisolone acetate weekly to activate chronic n. caninum infection. necropsies were performed 48, 17, 18, and 18 days respective ... | 1990 | 2382383 |
| diagnosis and treatment of neospora caninum infection in a dog. | neospora caninum, a protozoan organism, caused extensor rigidity of the pelvic limbs in a 12-week-old dog. diagnosis was based on results of muscle biopsy, neuroelectrodiagnostics, serotesting, and cell culture. indirect fluorescent antibody (ifa) titer to n caninum was 1:800 at time of admission and 1:3,200 after 4 and 6 weeks. a reciprocal ifa titer of 50 to n caninum was also found in the csf. serotesting for t gondii was negative. treatment with clindamycin followed by sulfadiazine and trime ... | 1990 | 2370226 |
| infection of mice with neospora caninum (protozoa: apicomplexa) does not protect against challenge with toxoplasma gondii. | neospora caninum and toxoplasma gondii are structurally related protozoal parasites of mammals that may cause abortion and neonatal morbidity and mortality. groups of mice were subcutaneously inoculated with 10(5) live zoites of the nc-1 or nc-3 isolates of n. caninum and reinoculated with an identical number of live zoites 2 weeks later. groups of mice which were injected subcutaneously with hanks balanced salt solution served as controls. three weeks after the final n. caninum inoculation, one ... | 1990 | 2370115 |
| neospora caninum infection in dogs. | 1990 | 2344341 | |
| effects of sulfadiazine and amprolium on neospora caninum (protozoa: apicomplexa) infections in mice. | an immunosuppressed mouse model was used to determine the effects of amprolium and sulfadiazine on experimental neospora caninum infections. both drugs were given in the drinking water. neither drug was effective in treating infections when given 7 days after inoculation of tachyzoites, when clinical signs of disease had developed. amprolium did not prevent deaths or development of clinical signs when given in the drinking water at 1 mg/ml or 5 mg/ml 3 days after inoculation of tachyzoites. sulf ... | 1990 | 2319416 |
| neospora caninum encephalomyelitis in a british dog. | 1990 | 2316150 | |
| fatal congenital neospora caninum infection in a lamb. | neospora caninum tissue cysts were found in the brain and spinal cord of a 1-wk-old lamb that was unable to stand after birth. the lamb was originally diagnosed as having toxoplasmosis, but ultrastructural and immunohistochemical techniques used in the present study permitted a definitive diagnosis of neospora caninum tissue cysts in the brain and spinal cord of this lamb. this is the first report of n. caninum in sheep. | 1990 | 2299518 |
| congenital neospora caninum infection in a calf with spinal cord anomaly. | neospora caninum was identified in a calf with spinal cord anomaly in australia. the calf was full-term and born dead. the caudal cervical and cranial thoracic segments of the spinal cord of the calf were asymmetric because of marked unilateral reduction of ventral gray matter and focal cavitation. mild focal disseminated nonsuppurative encephalomyelitis was associated with n caninum tissue cysts. tissue cysts were 16 to 35 microns x 10 to 27 microns, and the cyst walls were 1 to 3 microns thick ... | 1990 | 2243037 |
| neosporosis in cats. | six cats (nos. 1-6) were inoculated intramuscularly with (1 x 10(6)) and orally (5 x 10(5)) tachyzoites of neospora caninum. three (nos. 1-3) of the six cats were given 40 mg/kg methylprednisolone acetate 7 days before and on the day of inoculation with n. caninum tachyzoites, and three cats (nos. 4-6) were not given methylprednisolone acetate. two of the cats (cat nos. 1 and 2) given methylprednisolone acetate died suddenly. cat no. 1 died 8 days post-inoculation, and cat no. 2 died 16 days pos ... | 1990 | 2238386 |
| repeated transplacental transmission of neospora caninum in dogs. | four litters of german shorthaired pointers from one owner developed a toxoplasmosis-like illness. according to the records, 29 of 39 dogs had hind limb paralysis. six dogs from 2 litters were necropsied and had generalized encephalomyelitis. tachyzoites and tissue cysts of neospora caninum were found in the brain and spinal cord of each dog. lesions were found in the eyes, extraocular muscles, or both in all of the dogs, and n caninum was detected microscopically in the eyes (retina and choroid ... | 1990 | 2228766 |
| neospora caninum (apicomplexa) in an aborted equine fetus. | tachyzoites of neospora caninum were found in sections of lung of an equine fetus aborted 2 mo before term. individual tachyzoites were approximately 3-5 x 2-3 microns, divided by endodyogeny, and stained positively with anti-n. caninum serum but not with anti-toxoplasma gondii serum. toxoplasma gondii antibody was not found in the mare's serum. this is the first report of n. caninum in a horse and indicates that n. caninum can be transmitted transplacentally in equids. | 1990 | 2213418 |
| infections in mice with tachyzoites and bradyzoites of neospora caninum (protozoa: apicomplexa). | tachyzoites of 2 isolates of neospora caninum (nc-1 and nc-2) were inoculated subcutaneously (s.c.), intraperitoneally (i.p.), or orally into mice to compare the effects of route of inoculation on pathogenicity. mice developed more severe disease, and disease occurred sooner when inoculated with the nc-1 isolate compared to the nc-2 isolate. deaths occurred earlier in mice inoculated i.p. with either isolate. mice inoculated orally or s.c. with tachyzoites responded similarly to infection. tissu ... | 1990 | 2112599 |
| neospora caninum induced abortion in sheep. | 1990 | 2094452 | |
| neospora caninum-associated myocarditis and encephalitis in an aborted calf. | 1990 | 2090271 | |
| neospora-like protozoal infections associated with bovine abortions. | eighty bovine fetuses with presumed protozoal infections from a previous 2-year retrospective study were examined by immunohistochemistry using antisera against neospora caninum. in 66 (83%) of the fetuses, protozoa were found that reacted positively with anti-n. caninum sera. in three (4%) additional fetuses, protozoa identified as sarcocystis species did not react, and in two fetuses (3%) single protozoal clusters were found only in hematoxylin and eosin-stained slides. a group of 20 fetuses w ... | 1991 | 2063512 |
| neospora-like encephalomyelitis in a calf: pathology, ultrastructure, and immunoreactivity. | protozoal encephalomyelitis was diagnosed in a 3-day-old calf that was stunted, weak, and recumbent. grossly, the calf had contracted tendons in the forelegs, a slightly doomed skull, a porencephalic cyst in the cerebellum, ulcerative esophagitis, and abomasitis. histologically, there was a multifocal nonsuppurative encephalomyelitis with clusters of protozoal tachyzoites and numerous protozoal cysts. the porencephalic cyst and gastrointestional lesions appeared to be unrelated to the protozoal ... | 1991 | 2039786 |
| neospora-like protozoan infection as a major cause of abortion in california dairy cattle. | ninety-five aborted bovine fetuses received from california dairies over a 4.5-year period had histologic lesions of focal encephalitis. protozoa that reacted with neospora caninum antiserum were detected in the brain of 88 of these fetuses and in the heart of 1 fetus. sarcocystis spp schizonts were seen in the vascular endothelium of 1 fetus. it was concluded that a neospora-like cyst-forming coccidian may be a major cause of abortion in california dairy cattle. | 1991 | 2004983 |
| toxoplasma gondii-like schizonts in the tracheal epithelium of a cat. | toxoplasma gondii-like schizonts were found in tracheal epithelium of an 8-yr-old male cat. the parasites were located in parasitophorous vacuoles within the host cell cytoplasm, divided by schizogony, contained periodic acid-schiff-positive granules, and reacted with anti-t. gondii serum but not with anti-neospora caninum serum. mature schizonts were 7.0 x 5.9 microns (5-10 x 4-10 microns; n = 22) and contained 4-16 merozoites. the merozoites were approximately 5 x 1 microns. | 1991 | 1919933 |
| sarcocystis canis n. sp. (apicomplexa: sarcocystidae), the etiologic agent of generalized coccidiosis in dogs. | sarcocystis canis n. sp. is proposed for the protozoon associated with encephalitis, hepatitis, and generalized coccidiosis in dogs. only asexual stages are known in macrophages, neurons, dermal, and other cells of the body. the parasite is located free in the host cell cytoplasm without a parasitophorous vacuole; schizonts divide by endopolygeny. schizonts are 5-25 x 4-20 microns and contain 6-40 merozoites. merozoites are approximately 5-7 microns x 1 micron and do not contain rhoptries. the p ... | 1991 | 1907653 |
| sarcocystis neurona n. sp. (protozoa: apicomplexa), the etiologic agent of equine protozoal myeloencephalitis. | sarcocystis neuronan n. sp. is proposed for the apicomplexan taxon associated with myeloencephalitis in horses. only asexual stages of this parasite presently are known, and they are found within neuronal cells and leukocytes of the brain and spinal cord. the parasite is located in the host cell cytoplasm, does not have a parasitophorous vacuole, and divides by endopolygeny. schizonts are 5-35 microns x 5-20 microns and contain 4-40 merozoites arranged in a rosette around a prominent residual bo ... | 1991 | 1901359 |
| acute sarcocystosis-like disease in a dog. | two of 8 littermate rottweiler dogs developed persistent diarrhea at 6.5 weeks of age. dog 1 was euthanatized at 14 weeks of age and had hepatitis characterized by necrosis and mixed leukocyte infiltrations in association with a previously unrecognized sarcocystis-like protozoon. the organism was free in the hepatocyte cytoplasm without a parasitophorous vacuole, had divided by schizogony, and stained with anti-sarcocystis serum, but did not stain with anti-toxoplasma gondii or anti-neospora can ... | 1991 | 1901306 |
| [the clinical diagnosis of protozoal myositis syndrome (neospora caninum) of puppies]. | nine puppies suffered from progressive paresis with muscle wasting, hyporeflexia and extensor rigidity. ck-activity in serum was elevated and electrodiagnostic findings were indicative of lower motor neuron disease. although lesions were also found in the cns, additional neurological signs were rare, but csf examination revealed the presence of inflammatory lesions. on pathologic examination, all animals had a disseminated necrotizing myositis. in addition, a disseminated encephalomyelitis was f ... | 1991 | 1887444 |
| disseminated infection with neospora caninum in a ten-year-old dog. | naturally-occurring neosporosis with multiple organ involvement was identified in a 10-year-old neutered male basset hound dog. clinical signs were first noticed 3 weeks prior to referral and consisted of crouched stance and mild pelvic limb ataxia. dexamethasone administration had provided transient improvement. on presentation to the teaching hospital, clinical signs included depression, pelvic limb ataxia, inability to stand without assistance, and pain on deep palpation of the cervical and l ... | 1991 | 1879145 |
| evidence that neospora caninum is identical to the toxoplasma-like parasite of norwegian dogs. | 1991 | 1814191 | |
| paresis in dogs and neospora caninum. | 1991 | 1781143 | |
| factors affecting the survival of neospora caninum bradyzoites in murine tissues. | studies were conducted to determine factors that influence the survival of bradyzoites of neospora caninum within tissue cysts in the brains of experimentally inoculated mice. viable tissue cysts were detected in the brains of mice inoculated 13 mo previously with either of 2 isolates (nc-1 or nc-2) of n. caninum. bradyzoites within tissue cysts of the nc-2 isolate survived for at least 14 days at 4 c in refrigerated brain homogenates. bradyzoites within tissue cysts of the nc-2 isolate also sur ... | 1992 | 1738071 |
| neospora caninum (apicomplexa) in a stillborn goat. | tissue cysts of neospora caninum were found in sections of brain from a stillborn pygmy goat. the tissue cysts had 1-2-microns-thick cyst walls and stained with anti-n. caninum serum in an immunohistochemical test. glial nodules, mononuclear perivascular cuffing, and foci of inflammation were associated with n. caninum tissue cysts throughout the brain. this report indicates that n. caninum can be transmitted transplacentally in goats. | 1992 | 1597802 |
| an unidentified toxoplasma-like tissue cyst-forming coccidium in a cat (felis catus). | an unidentified coccidium was found in the brain and the heart of a 6-year-old female cat. tissue cysts measured 40-130 x 40-112 microns and contained hundreds of bradyzoites measuring 4.2-5.2 x 1.2-1.8 microns. the cyst wall was 0.26-0.53 microns thick. tissue cysts reacted positively with anti-toxoplasma gondii serum but not with anti-neospora caninum serum in an immunohistochemical test. ultrastructurally, the cyst was aseptate and contained banana-shaped bradyzoites. micronemes and rhoptries ... | 1992 | 1584745 |
| clinical neosporosis in a 4-week-old hereford calf. | most reports of neosporosis associated with abortion in cattle are in dairy cattle and infected calves do not survive beyond 7 days of age. this paper reports neosporosis in a 4-week-old hereford calf. the calf was full term and appeared clinically normal at birth. at 2 weeks of age, the calf had weakened and was unable to nurse unassisted. the calf was killed at 4 weeks because of paralysis. the primary lesions were in the brain and were associated with neospora caninum tachyzoites. the diagnos ... | 1992 | 1496796 |
| neospora caninum infection in english springer spaniel littermates. diagnostic evaluation and organism isolation. | progressive paraparesis developed in four male english springer spaniel pups from a litter of five during the first 10 weeks of life. two of the pups, which had the earliest onset of neurologic signs, were euthanatized without further workup. however, a detailed investigation was completed on the remaining two littermates at 12 weeks of age. both pups had progressive paraparesis for 3 to 4 weeks before presentation, with one dog developing subsequent asymmetric pelvic limb extensor rigidity. bas ... | 1992 | 1484374 |
| characterization of anti-neospora caninum hyperimmune rabbit serum by western blot analysis and immunoelectron microscopy. | the antigens recognized by hyperimmune rabbit serum raised against tachyzoites of the nc-1 isolate of neospora caninum were characterized using chemiluminescent western blotting, immunogold-silver staining and immunoelectron microscopy. approximately 20 immunodominant antigens whose relative rates of migration were 16-80 kda were recognized by the serum in western blots using reduced or nonreduced parasite antigen preparations. the nonreduced parasite antigens were more strongly recognized by th ... | 1992 | 1480607 |
| canine neosporosis in south africa. | neospora caninum was identified in tissues of one neonatal and two adolescent dogs by a specific immunohistochemical technique during a retrospective study. the histological lesions included multifocal polymyositis, multifocal parasitic myocarditis, interstitial pneumonia, severe multifocal encephalitis and myelitis with perivascular cuffing. this report extends the known range of the parasite to the african continent. | 1992 | 1466136 |
| neosporosis in the aborted fetus and newborn calf. | protozoal encephalitic lesions were found in four aborted fetuses and one dead newborn calf. the organism was identified as neospora caninum by immunoperoxidase. the brain lesions were of two forms. one was observed in three fetuses of 5 months gestation and was characterized by multifocal necrosis. the other was found in a 7-month fetus and in a newborn calf, and showed severe infiltration with macrophages and plasma cells containing igg. this association, between the age of fetus and inflammat ... | 1992 | 1452816 |
| [neospora abortion in cattle in the netherlands]. | three cases of bovine protozoal abortion at three different dairy farms are reported. multiple abortions had occurred, on two farms, and a sporadic abortion on the third farm. histopathological examination of foetal tissues revealed a distinctive multifocal necrotising encephalitis as well as focal inflammatory lesions in the myocardium, liver, kidney and lung. in one foetus protozoal tissue cysts were found in the brain and individual and clustered tachyzoites were seen in brain, and lung. in a ... | 1992 | 1440543 |
| induced transplacental transmission of neospora caninum in cattle. | three jersey cows were inoculated sc and im with 26 million neospora caninum tachyzoites at 129 (cow 1), 126 (cow 2), and 81 (cow 3) days after mating. cows remained clinically normal for at least 1 month after inoculation of n caninum. cow 1 was euthanatized 32 days after inoculation because of gangrenous mastitis. cow 1 had a live fetus with no gross lesions; however, microscopic lesions were seen in the fetus and consisted of severe nonsuppurative necrotizing encephalitis of the cerebral whit ... | 1992 | 1399772 |
| [abortions caused by protozoa in agricultural animals]. | a review is given on abortions in livestock caused by toxoplasma gondii, neospora caninum and sarcocystis spp. special emphasis is put on diagnostic procedures. t. gondii is considered to be a major cause of abortions in sheep and goats. diagnosis is based on the characteristic lesions in the cotyledons, histologic and immunohistochemical examination, and on the detection of specific antibodies in body fluids or serum of aborted fetuses. whether abortions in swine due to t. gondii are of practic ... | 1992 | 1396163 |
| development and characterization of monoclonal antibodies to first-generation merozoites of eimeria bovis. | merozoites of eimeria bovis were harvested from bovine monocyte cell cultures and used to immunize balb/c mice. spleens from immunized mice were removed and the cells fused with mouse myeloma cells. supernates from resulting hybridoma cell lines were examined for antibodies to first-generation e. bovis merozoites using an indirect immunofluorescent antibody (ifa) assay. three positive cell lines were identified and cloned by limiting dilution. all three cell lines produced immunoglobulins of the ... | 1992 | 1281590 |