PMID(sorted ascending)
seroprevalence of toxoplasma gondii and neospora caninum in goats from north-western spain.toxoplasma gondii and neospora caninum are protozoans involved in reproductive failure especially in ruminant livestock. the objective was to estimate the seroprevalence of both parasites in goats from north-western spain and to study the influence of some factors on seropositivity.201628030928
seroprevalence of toxoplasma gondii and neospora caninum in red deer from central italy.neospora caninum and toxoplasma gondii are cosmopolite protozoan parasites impacting on human and animal health. in particular, t. gondii commonly infects human beings and all warm-blooded animals, while n. caninum is responsible for bovine abortion and neuromuscular disease in dogs. the aim of the presented survey was to evaluate the occurrence and prevalence of these parasites in the most numerous italian red deer population. the sera of 60 red deer (cervus elaphus) inhabiting central italy (4 ...201628030947
the p38 mapk inhibitor, sb203580, inhibits cell invasion by neospora caninum.the apicomplexan parasite neospora caninum is an obligate intracellular parasitic protozoan. it can cause severe diseases in a number of animals throughout the world. infection with n. caninum leads to abortions in pregnant animals and neuromuscular disorders of newborns which cause great economic losses to animal husbandry. however, the mechanism of cell invasion by n. caninum is still unclear. this paper aims to investigate the impact of sb203580, a p38 mapk inhibitor, on host cell invasion by ...201728039498
evaluation of methylene blue, pyrimethamine and its combination on an in vitro neospora caninum model.neospora caninum is an apicomplexan parasite strongly related to reproductive problems in cattle. the neosporosis control is not well established and several fronts are under development, predominantly based on immune protection, immunomodulation and chemotherapy. the use of anti-malarial drugs as therapeutic sources has, in theory, considerable potential for any apicomplexan. drugs such as methylene blue (mb) and pyrimethamine (pyr) represent therapeutic options for malaria; thus, their use for ...201728073383
isolation and biological and molecular characterization of neospora caninum (nc-sp1) from a naturally infected adult asymptomatic cattle (bos taurus) in the state of são paulo, brazil.the biological and genetic diversity of neospora caninum is very limited because of availability of only a few viable isolates worldwide. this study describes the isolation and biological and molecular characterization of a new viable isolate of n. caninum (nc-sp1), from a cattle in brazil. approximately 400 g of brain from a naturally infected adult male cattle from an abattoir was fed to a 2-month-old dog. neospora-like oocysts were observed on day 7 post-inoculation (pi) and the duration of o ...201728073388
prevalence of bovine herpesvirus type 4 in aborting dairy cows.bovine herpesvirus type 4 (bhv-4) is related to many different conditions: infertility, postpartal metritis, vulvovaginitis, mastitis, encephalitis, calf pneumonia, keratoconjunctivitis, cutaneous lesions, digital dermatitis and abortion. in this study a retrospective pcr examination of 100 extracted dna samples from aborting cows was performed in order to determine: prevalence of bhv-4 in abortive cattle, whether coinfections bhv-4 with other abortifacient pathogens are present in the same samp ...201628092611
constitutive expression and characterization of a surface srs (ncsrs67) protein of neospora caninum with no orthologue in toxoplasma gondii.neospora caninum is a parasite of the apicomplexa phylum responsible for abortion and losses of fertility in cattle. as part of its intracellular cycle, the first interaction of the parasite with the target cell is performed with the surface proteins known as the srs superfamily (surface antigen glycoprotein - related sequences). sag related or srs proteins have been a target of intense research due to its immunodominant pattern, exhibiting potential as diagnostic and/or vaccine candidates. the ...201728108401
in contrast to toxoplasma gondii, neospora caninum tachyzoites did not sustain multiplication in vitro at increased incubation temperatures.neospora caninum and toxoplasma gondii are coccidian parasites that infect a wide range of mammalian and avian species. while viable t. gondii has been in vitro isolated in natural infections from wild and domestic birds, attempts to isolate n. caninum from naturally-infected birds were unsuccessful. we speculate that body temperatures of birds, which are usually higher than those of mammals, may impair the multiplication of n. caninum. in contrast to n. caninum, t. gondii can grow in vitro at t ...201728115178
a new microneme protein of neospora caninum, ncmic8 is involved in host cell invasion.microneme proteins play an important role in the invasion process of apicomplexan parasites through adhesion to host cells. we discovered a new n. caninum protein, ncmic8, which is highly identical to tgmic8. the ncmic8 sequence has 2049 bp and no intron in the open reading fragment. it has a molecular weight of 73.8 kda and contains a signal peptide, a transmembrane region, a low complexity region and 10 epidermal growth factor (egf) domains. immuno-fluorescence assay showed that ncmic8 is loca ...201728130119
effects of thai piperaceae plant extracts on neospora caninum infection.neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. this study investigated the effects of thai piperaceae (piper betle, p. nigrum, and p. sarmentosum) extracts on neospora caninum infections in vitro and in vivo. in an in vitro parasite growth assay based on the green fluorescent protein (gfp) signal, p. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (ic5 ...201728137670
two novel calcium-dependent protein kinase 1 inhibitors interfere with vertical transmission in mice infected with neospora caninum tachyzoites.we present the effects of two novel bumped kinase inhibitors, bki-1517 and bki-1553, against neospora caninum tachyzoites in vitro and in experimentally infected pregnant mice. these compounds inhibited tachyzoite proliferation of a transgenic beta-galactosidase reporter strain cultured in human foreskin fibroblasts with 50% inhibitory concentrations (ic50s) of 0.05 ± 0.03 and 0.18 ± 0.03 μm, respectively. as assessed by an alamarblue assay, fibroblast ic50s were above 20 μm; however, morphologi ...201728137808
uterine serpin (serpina 14) correlates negatively with cytokine production at the foetal-maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with neospora caninum.this study examines gene expression patterns in dairy heifers experimentally infected with n. caninum during on day 110 of pregnancy with live foetuses at euthanasia, 42 days later. the study population was constituted of four non-infected controls and three infected dams. gene expression was determined on gamma interferon (ifnγ), (th1 pro-inflammatory cytokine), interleukin-4 (il4) (th2 pro-gestation cytokine) or interleukin-10 (il10) (t regulatory cytokine) and the serine peptidase inhibitor s ...201728176427
a serosurvey of diseases of free-ranging gray wolves (canis lupus) in minnesota.we tested serum samples from 387 free-ranging wolves ( canis lupus ) from 2007 to 2013 for exposure to eight canid pathogens to establish baseline data on disease prevalence and spatial distribution in minnesota's wolf population. we found high exposure to canine adenoviruses 1 and 2 (88% adults, 45% pups), canine parvovirus (82% adults, 24% pups), and lyme disease (76% adults, 39% pups). sixty-six percent of adults and 36% of pups exhibited exposure to the protozoan parasite neospora caninum . ...201728192048
neospora caninum in beef herds in new south wales, australia. 1: seroprevalence determine the seroprevalence of neospora caninum antibodies in beef breeding herds across new south wales (nsw) and to determine if there are any differences associated with geographic location and other herd-level factors.201728239857
importance of serological cross-reactivity among toxoplasma gondii, hammondia spp., neospora spp., sarcocystis spp. and besnoitia besnoiti.toxoplasma gondii, neospora spp., sarcocystis spp., hammondia spp. and besnoitia besnoiti are genetically related cyst-forming coccidia. serology is frequently used for the identification of t. gondii, neospora spp. and b. besnoiti-exposed individuals. serologic cross-reactions occur in different tests among animals infected with t. gondii and h. hammondi, as well as among animals infected by t. gondii and n. caninum. infections caused by n. caninum and n. hughesi are almost indistinguishable by ...201728241894
controlled release of artemisone for the treatment of experimental cerebral malaria.cerebral malaria (cm) is a leading cause of malarial mortality resulting from infection by plasmodium falciparum. treatment commonly involves adjunctive care and injections or transfusion of artemisinins. all artemisinins that are in current use are metabolized to dihydroxyartemisinin (dha), to which there is already some parasite resistance. we used artemisone, a derivative that does not convert to dha, has improved pharmacokinetics and anti-plasmodial activity and is also anti-inflammatory (an ...201728249591
molecular analyses on neospora caninum-triggered netosis in the caprine system.neospora caninum is an obligate intracellular protozoan parasite causing serious reproductive disorders in large and small ruminants worldwide. polymorphonuclear neutrophils (pmn) react against multiple invading pathogens through different mechanisms including the release of neutrophil extracellular traps (nets). here, in vitro interactions of caprine pmn and n. caninum tachyzoites were studied. scanning electron microscopic- and immunofluorescence-analyses demonstrated that caprine pmn undergo ...201728254622
can hierarchical modeling improve our understanding of bovine abortion due to neospora caninum infection?the purpose of this study was to use mixed logistic regression to evaluate the association between neospora caninum serostatus and abortion, accounting for the effects of farms. the main objective was to explore the interpretation of this model and discuss the contribution of this analysis to our understanding of the disease's epidemiology. a mixed-effects logistic model using farms as a random effect and the serostatus for n. caninum, age of cattle and farm location as fixed effects was perform ...201728259557
the eurasian otter (lutra lutra) as a potential host for rickettsial pathogens in southern italy.canine monocytic ehrlichiosis and rickettsiosis are zoonotic tick-borne diseases of canids caused by the intracellular obligate bacteria ehrlichia canis and rickettsia species respectively. in this study, we investigated using standard and real-time pcr and sequencing, the occurrence and molecular characterization of e. canis and rickettsia species in the eurasian otter (lutra lutra) from the southern italian population. samples were screened by using molecular assays also for neospora caninum, ...201728267780
toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of neospora caninum experimentally infected cattle.innate immune responses at the maternal-fetal interface are key in the pathogenesis of neospora caninum, an obligate parasite that causes abortion in cattle. herein, we determined the gene expression of endosomal toll-like receptors (tlrs) in the placenta and fetuses from both non-infected pregnant heifers and pregnant heifers intravenously challenged with live tachyzoites of n. caninum on day 70 of gestation. on day 104 of pregnancy, mrna expression of tlrs 3 and 8, as well as that of tlrs 7 an ...201728288766
rhoptry protein 5 (rop5) is a key virulence factor in neospora caninum.neospora caninum, of the apicomplexa phylum, is a common cause of abortions in cattle and nervous system dysfunction in dogs. rhoptry proteins of apicomplexa play an important role in virulence. the objectives of this study were to study functions of ncrop5 in n. caninum by deleting the ncrop5 gene from the wild nc-1 strain. we selected ncrop5 in toxodb and successfully constructed an ncrop5 gene-deleted vector, ptcr-ncrop5-cd ko. then we screened the ncrop5 knockout strains (δncrop5) at the gen ...201728326073
dectin-1 compromises innate responses and host resistance against neospora caninum infection.neospora caninum is an intracellular protozoan parasite that has drawn increasing interest due to its association with worldwide repetitive bovine abortions, which cause billionaire losses to the meat and dairy industries annually. innate immunity plays an important role in infection control, and n. caninum activates the production of inflammatory mediators through toll-like receptors, nod-like receptors, and mitogen-activated protein kinase signaling pathways. advances in the knowledge of initi ...201728326085
identification and characterization of gra6/gra7 of neospora caninum in mdbk cells.neospora caninum, an apicomplexan parasite, is recognized as a major bovine abortifacient. dense granule antigens (gras) play important roles in the formation and modification of parasitophorous vacuoles (pvs) in toxoplasma gondii. however, a few studies investigating gras have been reported in n. caninum. the aim of the present study was to characterize the dense gra6/gra7 of n. caninum in pvs using mdbk cells as a host cell model. neospora caninum was inoculated into mdbk cells, and changes we ...201728338718
neospora caninum in beef herds in new south wales, australia. 2: analysis of risk determine the influence of farm-level and animal-level factors on the seroprevalence of antibodies to neospora caninum and associations between seropositivity and reproductive outcomes.201728346670
differential ifn-γ and tnf-α driven cytokine response distinguishes acute infection of a metatherian host with toxoplasma gondii and neospora caninum.toxoplasma gondii and neospora caninum (both apicomplexa) are closely related cyst-forming coccidian parasites that differ significantly in their host range and ability to cause disease. unlike eutherian mammals, australian marsupials (metatherian mammals) have long been thought to be highly susceptible to toxoplasmosis and neosporosis because of their historical isolation from the parasites. in this study, the carnivorous fat-tailed dunnart (sminthopsis crassicaudata) was used as a disease mode ...201728348050
widespread 5-methylcytosine in the genomes of avian coccidia and other apicomplexan parasites detected by an elisa-based date, little is known about cytosine methylation in the genomic dna of apicomplexan parasites, although it has been confirmed that this important epigenetic modification exists in many lower eukaryotes, plants, and animals. in the present study, elisa-based detection demonstrated that low levels of 5-methylcytosine (5-mc) are present in eimeria spp., toxoplasma gondii, cryptosporidium spp., and neospora caninum. the proportions of 5-mc in genomic dna were 0.18 ± 0.02% in e tenella sporulated ...201728361273
pathological findings in the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereutes procyonoides), with special emphasis on infectious and zoonotic agents in northern germany.anthropogenic landscape changes contributed to the reduction of availability of habitats to wild animals. hence, the presence of wild terrestrial carnivores in urban and peri-urban sites has increased considerably over the years implying an increased risk of interspecies spillover of infectious diseases and the transmission of zoonoses. the present study provides a detailed characterisation of the health status of the red fox (vulpes vulpes), stone marten (martes foina) and raccoon dog (nyctereu ...201728399176
prevalence of antibodies to neospora caninum in the serum of camels (camelus dromedarius) from central punjab, pakistan.infection with the apicomplexan parasite neospora caninum occurs in domestic and wild animals worldwide. this parasite causes significant economic losses in the dairy industry worldwide. the current study was designed to assess the prevalence of n. caninum antibodies in the sera of camels (camelus dromedarius) from rakh mahni, bhakkar, pakistan, to better understand the epidemiology of this important cause of bovine abortion in pakistan. eighty-one sera samples collected from apparently healthy ...201728470581
the coccidian parasites toxoplasma and neospora dysregulate mammalian lipid droplet biogenesis.upon infection, the intracellular parasite toxoplasma gondii co-opts critical functions of its host cell to avoid immune clearance and gain access to nutritional resources. one route by which toxoplasma co-opts its host cell is through hijacking host organelles, many of which have roles in immunomodulation. here we demonstrate that toxoplasma infection results in increased biogenesis of host lipid droplets through rewiring of multiple components of host neutral lipid metabolism. these metabolic ...201728487365
review on the immunological and molecular diagnosis of neosporosis (years 2011-2016).neosporosis, caused by the apicomplexan protozoan neospora caninum, is a disease which affects a wide range of mammalian hosts (mainly cattle and dogs). n. caninum infection is considered the major cause of livestock abortions worldwide, and therefore is responsible for great losses in the industry. because there are no effective treatments or vaccines, diagnosis is essential for pathogen control. studies of n. caninum mechanisms of pathogenesis have led to the identification of new antigens, in ...201728495191
neospora caninum abortion in a malayan tapir (tapirus indicus).a captive 17-year old female malayan tapir (tapirus indicus) aborted a fetus with a crown rump length of 19cm in early pregnancy. the fetus showed an early state of mummification. histologically, a multifocal mononuclear encephalitis, myocarditis and periportal hepatitis was present indicating a possible protozoal cause of abortion. although immunohistologically, neospora (n.) caninum antigen could not be demonstrated, n. caninum dna was detected by polymerase chain reaction (pcr) in brain, hear ...201728495194
seroprevalence of neospora caninum and toxoplasma gondii in exotic ruminants and camelids in the czech republic.neospora caninum and toxoplasma gondii are the protozoan parasites with definitive hosts from order carnivora. due to vertical transmission, both parasites can cause abortions and neonatal mortality that lead to significant productive and economic losses in the domestic ruminants. the aim of this study was to describe n. caninum and t. gondii seroprevalence in the group of frequently farmed captive exotic ruminants (n = 184) including bovidae (barbary sheep, bezoar goat, common eland, american b ...201728497227
peroxisome proliferator-activated receptor-γ-mediated polarization of macrophages in neospora caninum infection.neospora caninum is an apicomplexan parasite closely related toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. macrophage polarization plays an important role in host immune responses to parasites infection, such as toxoplasma gondii, leishmania, trypanosoma cruzi. however, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during n. caninum infection remains unclear.201728527905
nlrp3 inflammasome activation in murine macrophages caused by neospora caninum infection.neospora caninum is an intracellular parasite that causes significant economic losses in cattle industry. understanding the host resistance mechanisms in the innate immune response to neosporosis could facilitate the exploration of approaches for controlling n. caninum infection. the nlr inflammasome is a multiprotein platform in the cell cytoplasm and plays critical roles in the host response against microbes.201728558839
immune response in bovine neosporosis: protection or contribution to the pathogenesis of abortion.neospora caninum is a protozoan parasite with a preference for cattle and dogs as hosts. when n. caninum infection occurs in cattle it induces abortion, bovine neosporosis being a main cause of abortion worldwide. in dairy cattle, the economic burden of neosporosis-associated abortion is so great that it might results in closure of a farm. however, not all infected cows abort and it is not yet understood why this occurs. at present there is no effective treatment or vaccine. this review provides ...201728578088
molecular detection of neospora caninum infection in ovine aborted foetuses in the mashhad area, irann. caninum could cause abortion in small ruminants. the aim of the study was to detect n. caninum infection in ovine aborted foetuses in the mashhad area by pcr examination. during the period 2009 to 2013, 71 ovine aborted foetuses were collected and their brain samples examined by pcr. of the 71 brains of the aborted foetuses, n. caninum dna was detected in seven (9.8%) samples. in conclusion, it seems that n. caninum may act as a causative agent of abortion in sheep in the mashhad area.201728601062
seroprevalence of and risk factors for neospora caninum infection in yaks (bos grunniens) in china.the aim of the present study was to detect the seroprevalence of neospora caninum infection in yaks in gansu province, china. serum samples from 974 white yaks and 610 black yaks were tested by a commercial competitive-inhibition elisa for the detection of specific anti-n. caninum antibodies.; n. caninum antibodies were detected in 10.4% of yaks with higher prevalence in black (11.5% of 610) than white yak (8.6% of 974). age, regions and sampling times were considered as risk factors associated ...201728606319
peripheral and placental immune responses in goats after primoinfection with neospora caninum at early, mid and late gestation.neospora caninum can cause reproductive failure in goats. however, the pathogenesis of neosporosis in this domestic species remains largely unknown. we recently demonstrated that the outcome of experimental infection by n. caninum in pregnant goats is highly dependent on the time of gestation, during which infection occurs. in the present study, we examined the peripheral and placental immune responses in these groups of goats infected with 10(6) tachyzoites of the nc-spain7 isolate at early (g1 ...201728606322
bovine polymorphonuclear neutrophils cast neutrophil extracellular traps against the abortive parasite neospora caninum.neospora caninum represents a relevant apicomplexan parasite causing severe reproductive disorders in cattle worldwide. neutrophil extracellular trap (net) generation was recently described as an efficient defense mechanism of polymorphonuclear neutrophils (pmn) acting against different parasites. in vitro interactions of bovine pmn with n. caninum were analyzed at different ratios and time spans. extracellular dna staining was used to illustrate the typical molecules of nets [i.e., histones (h3 ...201728611772
new molecular tools in neospora caninum for studying apicomplexan parasite proteins.the development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum apicomplexa. we have established a system specifically designed for neospora caninum, and used this system as a heterologous platform for the expression of foreign genes. plasmid constructs containing fluorescent proteins or targeted genes of toxoplasma gondii, driven by n. caninum promoters, have yielded robust expression and correct trafficking of target gen ...201728630403
serology and protein electrophoresis for evidence of exposure to 12 mink pathogens in free-ranging american mink (neovison vison) in argentina.basic pathologic characteristics for farmed minks were previously reported worldwide. however, its status in the wild has not been studied in detail.201728635524
risk factors for neospora caninum infection in dairy cattle and their possible cause-effect relation for disease.neospora caninum causes reproductive problems in cattle such as abortion, premature birth, retention of fetal membranes, and metritis. therefore, this study aimed to verify possible risk factors for n. caninum infection in dairy cattle and their cause-effect relation to neosporosis. serum samples of 1518 dairy cows from the west of santa catarina state, southern brazil were analyzed by indirect immunofluorescence assay (ifa) for n. caninum, where 466 were found to be positives (30.69%-ci95%; 28. ...201728666842
serological survey of toxoplasmosis, neosporosis and brucellosis among cattle herds in oyo state, south-western nigeria.several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. this study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in oyo state, southwest nigeria.201728670646
incidence and vertical transmission rate of neospora caninum in sheep.the infection by neospora caninum in sheep can lead to abortion and the birth of weak and debilitated lambs. the aim of this study was to assess the incidence of natural infection by neospora caninum and the vertical transmission rate among sheep. a flock of 50 sheep was monitored for serum antibody titres against n. caninum and seroconversion over a period of six months using an indirect elisa technique. the offspring of the herd was also investigated regarding anti-n. caninum antibodies to det ...201728673457
serological survey of neospora caninum and toxoplasma gondii in cattle (bos indicus) and water buffaloes (bubalus bubalis) in ten provinces of brazil.the objective of this study was to determine the prevalence of antibodies to neospora caninum and toxoplasma gondii among 500 cattle (bos indicus) and 500 buffaloes (bubalus bubalis) using the indirect fluorescent antibody test (ifat) technique. blood samples from were collected from water buffalo and cattle in 10 municipalities in the northern region of brazil. the frequency of cattle and water buffaloes seropositive for neospora caninum in pará state, brazil, was 55% and 44%, respectively, and ...201728673459
towards a preventive strategy for neosporosis: challenges and future perspectives for vaccine development against infection with neospora caninum.neosporosis is caused by the intracellular protozoan parasite neospora caninum. this major disease-causing pathogen is responsible for inducing abortion in cattle, and these adverse events occur sporadically all over the world, including japan. currently, there are no vaccines on the market against infection with n. caninum. because live and attenuated vaccines against n. caninum have had safety and effectiveness issues, development of a next-generation vaccine is urgently required. to develop a ...201728690279
survey of neospora caninum in eared doves (zenaida auriculata) in southern brazil.neosporosis is an infectious disease caused by neospora caninum, a protozoan parasite that has worldwide distribution and is responsible for enormous economic losses in cattle. birds are considered a good bioindicator of environmental contamination, since they feed on the ground, being exposed to n. caninum oocysts. the aim of this study was to determine the occurrence of antibodies against n. caninum and to verify the presence of parasite dna in brain from free-ranging eared doves (zenaida auri ...201728716354
transduction of a neospora caninum antigen gene into mammalian cells using a modified bombyx mori nucleopolyhedrovirus for antibody production.autographa californica multiple nucleopolyhedrovirus (acmnpv) can easily enter and transduce foreign genes into mammalian cells, but these functions are difficult for bombyx mori nucleopolyhedrovirus (bmnpv). in this study, we investigated the induction of antibody production in mice immunized with an engineered bmnpv. the gp64 of bmnpv (bmgp64) was replaced with the gp64 of acmnpv (acgp64); this construct, designated bmnpvδbgp/acgp64, displays acgp64 on the surface of bmnpv. the neospora caninu ...201728716628
the enzymes and electrolytes profiles in sera of iranian stray dogs naturally infected with neospora caninumthe protozoan neospora caninum (apicomplexa: sarcocystidae), respectively infects canids and ruminants as the most definitive and intermediate hosts. a diagnosis of neosporosis is generally made on the basis of clinical signs together with the detection of high levels of antibodies in serum. the present study compares the serum electrolyte profile (ca, k, mg, na, p) and serum enzyme level (alp, ast, cpk) of non-infected dogs with those of stray dogs naturally infected with n. caninum. the indire ...201728741339
relationships between certain metabolic diseases and selected serum biochemical parameters in seropositive dairy cows against neospora caninum infection in different stages of lactationneospora caninum is an important cause of abortion in dairy cattle. the general health of affected cows has not been investigated before. therefore, the main objective of this study was to identify possible relationships between certain metabolic diseases and selected serum biochemical parameters in seropositive dairy cows against n. caninum antibodies in different stages of lactation. the study was carried out using 72 n. caninum seropositive cows and 61 seronegative dairy cows (control). serum ...201728741343
in vitro screening of the open source pathogen box identifies novel compounds with profound activities against neospora caninum.neospora caninum is a major cause of abortion in cattle and represents an important veterinary health problem of great economic significance. the medicines for malaria venture (mmv) pathogen box, an open-source collection of 400 compounds with proven anti-infective properties against a wide range of pathogens, was screened against a n. caninum beta-galactosidase reporter strain grown in human foreskin fibroblasts. a primary screening carried out at 1µm yielded 40 compounds that were effective ag ...201728751177
infection exposure, detection and causes of death in perinatal mortalities in polish dairy herds.the objective of this study was to determine the prevalence and types of infections in perinatal mortality (pm) cases from polish dairy farms and the relevance of the presence of infection to the cause of death. this prospective longitudinal study was carried out on 121 pm and 21 control calves with a gestation of ≥260 days. six control calves were euthanized and examined using the same protocol as for pm calves. material was collected over a 20-month period between november 2013 and june 2015. ...201728783589
association of neospora caninum with reproductive performance in dairy cows: a prospective study from iran.there is no prospective study from iran to estimate the direct risk of neospora caninum for pregnancy loss or reproductive factors. in addition, there is no report in the literature concerning the association of n. caninum with dystocia and sex of calves. therefore, this study was conducted on a group of dairy cows in a large intensive production system during 2011 to 2013 in southern iran to evaluate the impact of neosporosis on reproductive performance. a total of 253 cows which were diagnosed ...201728785385
activation of erk signaling via tlr11 induces il-12p40 production in peritoneal macrophages challenged by neospora caninum.neospora caninum, an obligate intracellular protozoan parasite, can infect a large variety of vertebrate hosts including the most economically important cattle. infection with n. caninum is a main cause of abortion in both dairy and beef cattle, which causes great economic losses worldwide. however, the mechanism of host cell infection by n. caninum has not been fully elucidated, especially in terms of inflammatory responses. in this study, the effect of tlr-erk signaling pathway on the synthesi ...201728798732
enrofloxacin and toltrazuril are able to reduce toxoplasma gondii growth in human bewo trophoblastic cells and villous explants from human third trimester pregnancy.classical treatment for congenital toxoplasmosis is based on combination of sulfadiazine and pyrimethamine plus folinic acid. due to teratogenic effects and bone marrow suppression caused by pyrimethamine, the establishment of new therapeutic strategies is indispensable to minimize the side effects and improve the control of infection. previous studies demonstrated that enrofloxacin and toltrazuril reduced the incidence of neospora caninum and toxoplasma gondii infection. the aim of the present ...201728798905
toxoplasma gondii and neospora caninum induce different host cell responses at proteome-wide phosphorylation events; a step forward for uncovering the biological differences between these closely related parasites.toxoplasma gondii and neospora caninum are closely related intracellular protozoan parasites and tissue cyst-forming coccidia of the phylum apicomplexa. there are remarkable similarities between the morphology, genomes and transcriptomes of both parasites. toxoplasma is zoonotic, with a wide host range and is mainly transmitted horizontally between its definitive host, the cat, and its intermediate hosts. neospora causes disease within a narrow host range and with reduced virulence potential to ...201728803361
immunization with inactivated antigens of neospora caninum induces toll-like receptors 3, 7, 8 and 9 in maternal-fetal interface of infected pregnant heifers.neospora caninum is an obligate parasite and a major cause of abortion in cattle. pregnancy failures appear to be associated with weak innate defences on the maternal-fetal interface during infection with n. caninum. herein, we studied the gene expression of toll-like receptors (tlrs) in pregnant heifers immunized with different vaccine formulations against n. caninum before mating and then challenged the heifers with live n. caninum on day 70 of gestation. tlr7 and tlr8 expression was upregulat ...201728807280
neospora caninum rop16 play an important role in the pathogenicity by phosphorylating host cell stat3.neospora caninum is a common cause of abortions in cattle and nervous system dysfunctions in dogs. our analysis shows that ncrop16 and tgrop16 have similar structures and may have similar functions. to our surprise, we found that similar to the t. gondii rh strain, the n. caninum nc-1 strain could phosphorylate stat3(y705), but in contrast to t. gondii, n. caninum nc-1 could not phosphorylate stat6(y641). we constructed a gene-knockout plasmid and screened δncrop16 strains at the gene, protein a ...201728807283
sarcocystis neurona and neospora caninum in brazilian opossums (didelphis spp.): molecular investigation and in vitro isolation of sarcocystis spp.sarcocystis neurona and neospora spp. are protozoan parasites that induce neurological diseases in horses and other animal species. opossums (didelphis albiventris and didelphis virginiana) are definitive hosts of s. neurona, which is the major cause of equine protozoal myeloencephalitis (epm). neospora caninum causes abortion in cattle and infects a wide range of animal species, while n. hughesi is known to induce neurologic disease in equids. the aims of this study were to investigate s. neuro ...201728807293
a rare case of acute toxoplasmosis in a stray dog due to infection of t. gondii clonal type i: public health concern in urban settings with stray animals?typing of toxoplasma gondii strains is important in epidemiological surveys, to understand the distribution and virulence of different clones of the parasite among human and animal populations. stray dogs can be consider sentinel animals for contaminated environments playing an important but probably under- evaluated role in the epidemiology of t. gondii. we reported a rare case of acute toxoplasmosis in a stray dog due to clonal type i infection. the clonal type i, sporadic in europe, is freque ...201728818085
engineering and purification of a thermostable, high-yield, variant of pfcrt, the plasmodium falciparum chloroquine resistance transporter.historically chloroquine was used to treat the most deadly form of malaria, caused by the parasite plasmodium falciparum. the selective pressure of chloroquine therapy led to the rapid emergence of chloroquine resistant parasites. resistance has been attributed to the plasmodium falciparum chloroquine resistance transporter (pfcrt), an integral membrane protein of unknown structure. a pfcrt structure would provide new insights into how the protein confers chloroquine resistance and thereby also ...201728823509
development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time pcr for the rapid visual detection of canine neosporosis.dogs are the definitive hosts of neospora caninum and play an important role in the transmission of the parasite. despite the high sensitivity of existing molecular tools such as quantitative real-time pcr (qpcr), these techniques are not suitable for use in many countries because of equipment costs and difficulties in implementing them for field diagnostics. therefore, we developed a simplified technique, loop-mediated isothermal amplification (lamp), for the rapid visual detection of n. caninu ...201728835287
herd-level prevalence of selected endemic infectious diseases of dairy cows in great implement appropriate and effective disease control programs at the national level, up-to-date and unbiased information on disease frequency is needed. the aim of this study was to estimate the prevalence of selected endemic infectious diseases in the population of dairy herds in great britain. bulk milk tank (bmt) samples from 225 randomly selected dairy farms, stratified by region and herd size, were tested for antibodies against bovine viral diarrhea virus (bvdv), bovine herpesvirus type 1 ...201728843682
host species heterogeneity in the epidemiology of nesopora caninum.pathogen transmission across species drives disease emergence; however, mechanisms by which multi-host pathogens cross species boundaries are not well identified. this knowledge gap prevents integrated and targeted control in an epidemiologically continuous ecosystem. our goal is to describe the impact of host species heterogeneity on the epidemiology of neospora caninum circulating between livestock and wildlife in southeastern ohio. we collected biological samples from père david's deer (elaph ...201728850580
Displaying items 1801 - 1864 of 1864