| development of a microtiter plate hybridization-based pcr-enzyme-linked immunosorbent assay for identification of clinically relevant human group a rotavirus g and p genotypes. | a microtiter plate hybridization-based pcr-enzyme-linked immunosorbent assay (pcr-elisa) has been used for the detection and identification of a variety of microorganisms. here, we report the development of a pcr-elisa for the identification of clinically relevant human rotavirus vp7 (g1 to g6, g8 to g10, and g12) and vp4 (p[4], p[6], p[8], p[9], and p[14]) genotypes. the g and p types of reference human and animal rotavirus strains for which specific probes were available were correctly identif ... | 2008 | 18057127 |
| [comparison of clinical characteristics of patients with rotavirus gastroenteritis relative to the infecting rotavirus g-p genotype.]. | group a rotavirus is a major cause of severe diarrhea in children throughout the world. for the proper management of rotavirus infections, it will be helpful to know their clinical characteristics according to the g and p genotypes of the infecting virus. | 2006 | 18156706 |
| prevalence of enteropathogenic viruses and molecular characterization of group a rotavirus among children with diarrhea in dar es salaam tanzania. | different groups of viruses have been shown to be responsible for acute diarrhea among children during their first few years of life. epidemiological knowledge of viral agents is critical for the development of effective preventive measures, including vaccines. | 2007 | 18162127 |
| development of a method for concentrating and detecting rotavirus in oysters. | identification of enteric viruses in outbreak-implicated bivalve shellfish is difficult because of low levels of contamination and natural inhibitors present in shellfish tissue. in this study, the acid adsorption-alkaline elution method developed in our laboratory was proposed for the detection of rotavirus from oyster samples. the acid adsorption-alkaline elution process included the following steps: acid adsorption at ph 4.8, elution with 2.9% tryptose phosphate broth containing 6% glycine, p ... | 2008 | 18177969 |
| expression and characterization of vp2 protein of human rotavirus a in a mammalian lung cell line. | a system for the expression and characterization of vp2 protein of human rotavirus a, strain g3 was established in the mammalian lung cell line (a549). expression of the recombinant vp2 (rvp2) protein was detected 48-72 hrs after transfection by western blotting. the rvp2 protein expressed in a549 cells formed intracellular core-like particles (clps) 30 nm in diameter detected by electron microscopy (em). these results showed that the a549 cells are suitable as efficient eukaryotic host for prod ... | 2007 | 18197733 |
| rearrangements of rotavirus genomic segment 11 are generated during acute infection of immunocompetent children and do not occur at random. | group a rotaviruses are the main cause of viral gastroenteritis in infants. the viral genome consists of 11 double-stranded rna (dsrna) segments. dysfunction of the viral rna polymerase can lead to gene rearrangements, which most often consist of partial sequence duplication of a dsrna segment. gene rearrangements have been detected in vivo during chronic infection in immunodeficient children or in vitro during passages at a high multiplicity of infection in cell culture, suggesting that these r ... | 2008 | 18216096 |
| full genome-based classification of rotaviruses reveals a common origin between human wa-like and porcine rotavirus strains and human ds-1-like and bovine rotavirus strains. | group a rotavirus classification is currently based on the molecular properties of the two outer layer proteins, vp7 and vp4, and the middle layer protein, vp6. as reassortment of all the 11 rotavirus gene segments plays a key role in generating rotavirus diversity in nature, a classification system that is based on all the rotavirus gene segments is desirable for determining which genes influence rotavirus host range restriction, replication, and virulence, as well as for studying rotavirus epi ... | 2008 | 18216098 |
| molecular characterization of a subgroup specificity associated with the rotavirus inner capsid protein vp2. | group a rotaviruses are classified into serotypes, based on the reactivity pattern of neutralizing antibodies to vp4 and vp7, as well as into subgroups (sgs), based on non-neutralizing antibodies directed against vp6. the inner capsid protein (vp2) has also been described as a sg antigen; however, little is known regarding the molecular determinants of vp2 sg specificity. in this study, we characterize vp2 sgs by correlating genetic markers with the immunoreactivity of the sg-specific monoclonal ... | 2008 | 18216104 |
| molecular characterization of group a human rotaviruses in bangkok and buriram, thailand during 2004-2006 reveals the predominance of g1p[8], g9p[8] and a rare g3p[19] strain. | the aim of this study has been to determine the incidence of diverse human rotavirus strains circulating in thailand between october 2004 and april 2006 by means of molecular characterization. pediatric patients aged between 2 months and 5 years diagnosed with acute diarrhea (n=307) in bangkok and buriram, thailand were tested for human rotavirus a (rv-a) by rt-pcr. a total of 130 specimens (42.3%) were found rv-a positive and 126 were characterized by direct sequencing of the capsid glycoprotei ... | 2008 | 18228123 |
| multiple combinations of p[13]-like genotype with g3, g4, and g5 in porcine rotaviruses. | epidemiological surveillance of porcine rotavirus (porv) strains was carried out in chiang mai province, thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by pcr. of these, six were g3 and one was g4 and displayed a p-nontypeable genotype, while another isolate was both g and p nontypeable. analysis of a partial vp4 gene of all eight p-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged ... | 2008 | 18234859 |
| development of a rapid and sensitive latex agglutination-based method for detection of group a rotavirus. | considering the background of morbidity and mortality caused by human rotavirus, detection methods that use rotavirus group antigen (vp6) in either enzyme immunoassay (eia) or latex agglutination test (lat) has been employed routinely in clinical diagnostic and epidemiological studies. in order to develop a rapid and sensitive rotavirus group a lat, part of segment 6 corresponding to conserved n-terminal portion of the vp6 (1-245 aa) was cloned in escherichia coli expression pgex vector (glutath ... | 2008 | 18241934 |
| emergence of human rotavirus group a genotype g9 strains, wuhan, china. | of 322 stool specimens collected from children with diarrhea from october 2005 through september 2006 in wuhan, china, group a rotavirus was identified in 101 (31.4%). the most prevalent group a rotavirus genotype was g3p[8] (62.6%), followed by g1p[8](17.6%), g1+g3p[8](8.8%), g3p[4](6.6%), g1p[4](2.2%), and g9p[8](2.2%). the g9 strains were first detected in wuhan. | 2007 | 18258015 |
| is rotavirus a hepatotropic virus? | | 2008 | 18264761 |
| [genetic variation in vp7 gene of rotavirus serotype g3 predominated in changchun, china]. | group a rotavirus (rv) is the most important etiologic agent of severe gastroenteritis among children and the development of an effective vaccine becomes the top public health priority. since survey of rv serotypes circulating in local community is important for introduction or development of rv vaccine, rv serotype g3 had proved as the predominant strain in changchun from 2001 to 2005. stool specimens collected from children with acute diarrhea were tested for group a rotavirus by enzyme-linked ... | 2008 | 18320818 |
| full genomic analysis of human rotavirus strain tb-chen isolated in china. | a g2p[4]/nsp4[a] rotavirus strain tb-chen was isolated from a 2-year-old patient hospitalized with acute gastroenteritis in kunming, china. the strain tb-chen was demonstrated having group a-specific antigenicity, a "short" (subgroup ii) electropherotype. to investigate its overall genomic relatedness and to determine which group it belonged, the complete genome of strain tb-chen was determined. genomic comparison based on amino acid sequence identity and phylogenetic analysis revealed that all ... | 2008 | 18329063 |
| detection and characterization of human caliciviruses associated with sporadic acute diarrhea in adults in djibouti (horn of africa). | recent advances in molecular diagnostics have allowed us to recognize human caliciviruses (hucvs) as important agents of acute diarrhea in industrialized countries. their prevalence and genetic diversity in developing countries remains unknown. we report on the characterization of hucvs among adults presenting acute diarrheas in djibouti; 108 stool samples collected were screened by eia, rtpcr, or cell cultures for the group a rotaviruses, adenoviruses, astroviruses, and hucvs, which were furthe ... | 2008 | 18337354 |
| design of a multiplex nested pcr for genotyping of the nsp4 from group a rotavirus. | a novel pcr method was developed to discriminate amongst genotypes a-c of the rotavirus non-structural protein 4 (nsp4). genotype-specific primers were designed that correctly identified the nsp4 genotype when evaluated as a multiplex pcr with cell culture adapted rotavirus strains. rotavirus strains b223 sgig6p6[1], ncdv sgig6p6[1] and sa11 sgig3p5b[2] were used as control for nsp4 genotype a; a34 sgig5p14[23], gottfried sgiig4p2b[6] and wa sgiig1p1a[8] for nsp4 genotype b; rrv sgig3p5b[3] for ... | 2008 | 18353449 |
| porcine rotavirus bearing an aberrant gene stemming from an intergenic recombination of the nsp2 and nsp5 genes is defective and interfering. | serial undiluted passage of a porcine rotavirus in ma104 cells yielded three distinct virus populations, each of which bore different rearranged genes. sequencing revealed that each of two populations bore a distinct intragenic recombinant nsp3 gene consisting of a partial duplication in a head-to-tail orientation without altering the nsp3 open reading frame and the third population carried both an intragenic recombinant nsp3 gene and an intergenic recombinant gene (1,647 nucleotides in length) ... | 2008 | 18417592 |
| hospitalization due to norovirus and genotypes of rotavirus in pediatric patients, state of espírito santo. | viruses are the leading cause for hospitalization due to gastroenteritis worldwide. group a rotaviruses (rv) are the most prevalent and are assorted in glycoproteins (g) and protease sensitive (p) dual genotypes based on polymorphic genes that encode the external vp7 and vp4 capsid proteins, respectively. noroviruses (nov) have increasingly answered by sporadic gastroenteritis. this study aimed to determine the prevalence of nov and rv in 68 hospitalized children, between july 2004 and november ... | 2008 | 18425274 |
| [epidemiology and burden of rotavirus diarrhea in day care centers in lyon, france]. | rotavirus is the main cause of severe, dehydrating diarrhoea in infants and young children. in industrialized countries, pediatric rotavirus gastroenteritis (prge) is responsible for high morbidity, particularly among children under 3 years of age attending day care centers (dccs). the objectives of this study were to estimate the incidence, management and cost of prge in dccs. we also described the nature of group a rotavirus genotypes. this study also compared the performance of different diag ... | 2008 | 18456480 |
| occurrence of group a rotavirus mixed p genotypes infections in children living in goiânia-goiás, brazil. | group a rotaviruses (rva) are the main causing agents of acute gastroenteritis worldwide, having a great impact on childhood mortality in developing countries. the objective of this study was to identify rva-positive fecal samples with mixed p genotypes by hemi-nested reverse transcriptase-polymerase chain reaction (rt-pcr), followed by sequencing confirmation. our results showed that, from the 81 rva-positive samples, 25 were positive for more than one p genotype by hemi-nested rt-pcr. of these ... | 2008 | 18521634 |
| rotavirus infection in children and adult patients attending in a tertiary hospital of são paulo, brazil. | during the period of january 2003 to december 2005, 3,768 stool samples were received in the microbiology laboratory for rotavirus antigen detection from outpatients and inpatients of albert einstein hospital, sp. fresh stool samples from children and adults were analyzed by two methodologies: during 2003 and 2004 by latex agglutination (slidex rotavirus, biomerieux) and 2005 by an immunochromatographic assay for the combined detection of rotavirus and adenovirus (vikia rota-adeno, biomerieux). ... | 2008 | 18553013 |
| human, porcine and bovine rotaviruses in slovenia: evidence of interspecies transmission and genome reassortment. | a surveillance of human, porcine and bovine rotaviruses was carried out in slovenia in 2004 and 2005. stool samples were collected from a total of 406 pigs (373 from asymptomatic animals), 132 cattle (126 from asymptomatic animals) and 241 humans (all with diarrhoea), tested for group a rotaviruses using rt-pcr and analysed by sequencing. the aims of the study were to determine the incidence of asymptomatic rotavirus infection in animals, to look for evidence of zoonotic transmission and to dete ... | 2008 | 18559940 |
| molecular characterization of the nsp4 gene of human group a rotavirus samples from the west central region of brazil. | nonstructural protein 4 (nsp4), encoded by group a rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. the nsp4 gene has been sequenced, and five distinct genetic groups have been described: genotypes a-e. nsp4 genotypes a, b, and c have been detected in humans. in this study, the nsp4-encoding gene of human rotavirus strains of different g and p genotypes collected from children between 1987 and 2003 in three cities of west central regio ... | 2008 | 18592101 |
| recommendations for the classification of group a rotaviruses using all 11 genomic rna segments. | recently, a classification system was proposed for rotaviruses in which all the 11 genomic rna segments are used (matthijnssens et al. in j virol 82:3204-3219, 2008). based on nucleotide identity cut-off percentages, different genotypes were defined for each genome segment. a nomenclature for the comparison of complete rotavirus genomes was considered in which the notations gx-p[x]-ix-rx-cx-mx-ax-nx-tx-ex-hx are used for the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5/6 encoding genes, resp ... | 2008 | 18604469 |
| llama-derived single-chain antibody fragments directed to rotavirus vp6 protein possess broad neutralizing activity in vitro and confer protection against diarrhea in mice. | group a rotavirus is one of the most common causes of severe diarrhea in human infants and newborn animals. rotavirus virions are triple-layered particles. the outer capsid proteins vp4 and vp7 are highly variable and represent the major neutralizing antigens. the inner capsid protein vp6 is conserved among group a rotaviruses, is highly immunogenic, and is the target antigen of most immunodiagnosis tests. llama-derived single-chain antibody fragments (vhh) are the smallest molecules with antige ... | 2008 | 18632867 |
| detection and characterization of group c rotaviruses in asymptomatic piglets in ireland. | group c rotaviruses are important human enteric pathogens that have also been detected in a variety of mammalian species, including pigs. group c rotaviruses have been identified in piglets with diarrhea, but their ecology remains to be elucidated. by screening of 292 fecal samples collected from 4- to 5-week-old asymptomatic pigs from four herds in ireland between 2005 and 2007, 13 (4.4%) samples tested positive by reverse transcription-pcr for group c rotavirus. group a rotaviruses were also d ... | 2008 | 18632912 |
| outbreak of rotavirus variant p[8] in seoul, south korea. | an epidemiologic study was performed to determine the genetic variability of rotaviruses in seoul, south korea. in 3,174 stool specimens from children with acute diarrhea at five referral hospitals, 571 (18%) possessed the antigen of group a rotavirus detectable by elisa--10.8% in 2004 and 28.1% in 2005. vp7 genotyping revealed that the g3 type was found in 25.6% of all typed isolates, g4 in 23.8%, g2 in 21.6%, and g1 in 17.6%. vp4 genotyping showed that the p[8] type was detected in 66.7%, p[6] ... | 2008 | 18649327 |
| incidence and clinical characteristics of group a rotavirus infections among children admitted to hospital in kilifi, kenya. | rotavirus, predominantly of group a, is a major cause of severe diarrhoea worldwide, with the greatest burden falling on young children living in less-developed countries. vaccines directed against this virus have shown promise in recent trials, and are undergoing effectiveness evaluation in sub-saharan africa. in this region limited childhood data are available on the incidence and clinical characteristics of severe group a rotavirus disease. advocacy for vaccine intervention and interpretation ... | 2008 | 18651787 |
| genomic characterization of a novel group a lamb rotavirus isolated in zaragoza, spain. | an ovine rotavirus (ovr) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in zaragoza, spain, and the vp4, vp7, vp6, nsp4, and nsp5/nsp6 genes were subsequently characterized molecularly. strain ovr762 was classified as a p[14] rotavirus, as the vp4 and vp8* trypsin-cleavage product of the vp4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the p11[14] human rotavirus (hrv) strain pa169, isolated in italy. analy ... | 2008 | 18661221 |
| determination of human rotavirus vp6 genogroups i and ii by reverse transcription-pcr. | based on nucleotide sequence and phylogenetic analysis of the partial vp6 genes, group a rotaviruses can be mainly differentiated into two genogroups. in this study, a method employing reverse transcription-pcr (rt-pcr) and degenerate primers was established to assign the vp6 genogroup. vp6 genogroup i and genogroup ii could be determined according to the sizes of the amplicons: 380 and 780 bp, respectively. the vp6 genogroup of human reference strains of g1 to g4 and g9 types and rotateq vaccin ... | 2008 | 18667595 |
| molecular characterization of vp4, vp6 and vp7 genes of a rare g8p[14] rotavirus strain detected in an infant with gastroenteritis in italy. | in this study, the molecular characterization of a rare g8p[14] group a rotavirus (garv) strain detected in northern italy during the 2004-2005 epidemiological rotavirus season is described. two hundred and seventy three rotavirus-like particle positive stools out of 856 stools from children (31.9%) hospitalized with gastroenteritis were analyzed using polyacrilamide gel electrophoresis and 271 garvs were genotyped by vp7 and vp4 specific rt-pcrs. one strain (pr/1300/04) with a long electrophero ... | 2008 | 18674577 |
| molecular characterization of rare bovine group a rotavirus g15p[11] and g15p[21] strains from eastern india: identification of simian sa11-like vp6 genes in g15p[21] strains. | during a surveillance study (november 2001-march 2005), one rare g15p[11] and two rare g15p[21] bovine group a rotavirus strains were detected in diarrhoeic calves in eastern india. sequence analysis of the vp8*, vp6, nsp4 and nsp5 genes of the g15p[11] strain confirmed its bovine origin. although the nsp4 and nsp5 genes of the two g15p[21] strains were of bovine origin, their vp6 genes shared higher nucleotide and amino acid identities with simian strain sa11 (92.5-93.1% and 98.5-98.7%) than bo ... | 2008 | 18679786 |
| a survey of specific pathogen-free chicken flocks for antibodies to chicken anaemia agent, avian nephritis virus and group a rotavirus. | sera originating from specific pathogen-free flocks from europe, north america and australia were examined by indirect immuno-fluorescence for antibodies to chicken anaemia agent, avian nephritis virus and group a rotavirus. some flocks were free of infection, but others showed evidence of infection with one, two or all of these viruses. in view of the pathogenic potential of these viruses and the possibility that they may be egg-transmitted, it is recommended that their elimination from specifi ... | 1989 | 18679854 |
| vp6 capsid protein of chicken rotavirus strain ch2: sequence, phylogeny and in silico antigenic analyses. | the inner capsid protein vp6 of group a rotavirus possesses group and subgroup epitope specificities. avian rotaviruses have a unique vp6 that is antigenically different from its mammalian counterpart. the lack of information on the vp6 protein of chicken rotavirus strain, ch2, at the genetic and antigenic level was a major motivation for this work. sequencing of the complete cdna of the vp6 gene of ch2, revealed a nucleotide (amino acid) identity that varied from 78.3 to 98.5% (86.4-98.2%) when ... | 2008 | 18680770 |
| molecular characterization of equine rotavirus in ireland. | group a rotaviruses are important causative agents of severe, acute dehydrating diarrhea in foals. a total of 86 rotavirus-positive fecal samples, collected from diarrheic foals from 11 counties in three of the four provinces of ireland, were obtained from the irish equine centre in kildare during a 7-year (1999 to 2005) passive surveillance study and were characterized molecularly to establish the vp7 (g type) and vp4 (p type) antigenic specificities. fifty-eight samples (67.5%) were found to c ... | 2008 | 18716232 |
| the post-tsunami outbreak of diarrhoeal diseases in car nicobar island, india, was caused by human group a rotavirus g2 strains. | | 2008 | 18723226 |
| increase in prevalence of human group a rotavirus g9 strains as an important vp7 genotype among children in eastern india. | in addition to four globally important group a rotavirus (garv) vp7 genotypes (g1-g4), recent surveillance studies have revealed importance of g9 strains as an aetiological agent of infantile diarrhoea. | 2008 | 18755625 |
| electrophoretic rna genomic profiles of rotavirus strains prevailing among hospitalized children with acute gastroenteritis in tehran, iran. | human rotavirus has now been established as the most important cause of childhood gastroenteritis worldwide. the rna genomic electrophoretic diversity of group a rotavirus strains in tehran, iran, during april 2002 through march 2005, was investigated in 1250 stool specimens. | 2008 | 18759520 |
| evaluation of rapid assays for the detection of bovine coronavirus, rotavirus a and cryptosporidium parvum in faecal samples of calves. | rapid immunochromatographic assays for detecting infections with bovine coronavirus (bcv), rotavirus a and cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (bcv and rotavirus) and a sedimentation-flotation technique (c. parvum). rapid tests for the detection of bcv and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). sensitivity and s ... | 2009 | 18778958 |
| prospective study of etiologic agents of acute gastroenteritis outbreaks in child care centers. | to investigate the etiology of outbreaks of acute gastroenteritis (age) in children attending childcare centers (cccs) in north carolina between october 2005 and march 2007. | 2009 | 18783794 |
| human group a rotavirus p[8] hun9-like and rare op354-like strains are circulating among diarrhoeic children in eastern india. | during 2004-2006, group a rotavirus p[8] strains were the major vp4 genotype (43.2%, n = 317) among diarrhoeic children in eastern india. phylogenetic analysis of vp8* amino acid sequences of 16 of these strains with other p[8] strains revealed four distinct lineages. p[8] strains from eastern india clustered within rare op354-like and hun9-like lineages, pointing towards co-prevalence of divergent p[8] strains. although it is unclear whether the observed genetic diversity might affect to some e ... | 2008 | 18787756 |
| a novel genomic constellation (g10p[3]) of group a rotavirus detected from buffalo calves in northern india. | group a bovine rotaviruses cause gastroenteritis and calf mortality leading to significant economic losses to dairy farmers in india. due to segmented nature of the rna genome and wide host range, vast genetic and antigenic diversity exists among different isolates of rotavirus. molecular characterization of locally prevalent group a rotavirus strains in buffalo population in north india was undertaken. out of a total of 455 faecal samples, 21 samples (4.61%) were positive for bovine rota virus ... | 2008 | 18793686 |
| an outbreak of group a rotavirus g1p[8] in an elementary school, nara prefecture. | | 2008 | 18806362 |
| emergence of the gii4/2006b variant and recombinant noroviruses in china. | noroviruses are an important cause of acute gastroenteritis. increasing data showed that the gii-4 strains are predominant worldwide and new gii-4 variants emerge every 1-2 years causing major epidemics. surveillance of gastroenteritis in hospitalized children under 5 years of age in china is described. among 1,110 specimens, 114 (10.3%) were positive for noroviruses, which was higher than adenoviruses (7.6%), astroviruses (3.5%), and sapoviruses (0.9%) and only lower than group a rotaviruses (4 ... | 2008 | 18814250 |
| molecular characterization of vp6-encoding gene of group a human rotavirus samples from central west region of brazil. | group a rotaviruses are the main cause of acute gastroenteritis in children worldwide. the intermediate capsid protein vp6 encoded by segment 6 of the dsrna genome is the major structural component of the virus and it is highly antigenic and immunogenic. vp6 is responsible for group and subgroup (sg) specificities, allowing classification of group a rotavirus into sg i, sg ii, sg i + ii, and sg non-i-non-ii. vp6-encoding gene of 154 group a human rotavirus samples of different g and p genotypes ... | 2008 | 18814260 |
| [rotaviruses in younger children in novosibirsk in 2005-2007: detection and genotyping]. | examination of 1898 patients with acute enteric infection from march 2005 to february 2007 showed that group a rotaviruses were the most frequent cause (35%) of acute gastroenteritis among children under 3 years of age. majority of cases of rotavirus infection was detected in infants under 1 year of age (71.8%). the peak of sporadic incidence was observed between february and may. high rate of mixed infection (45.6%) was observed - associations of rotaviruses with other viruses (noroviruses, ast ... | 2008 | 18819400 |
| use of polymerase chain reaction for the differentiation of group a bovine rotavirus g6, g8, and g10 genotypes in the north island of new zealand. | to determine the presence of group a rotavirus g6, g8, and g10 genotypes in calves in the north island of new zealand. | 2008 | 18836501 |
| unexpected substitution of dominant rotavirus g genotypes in french hospitalized children over five consecutive seasons. | the study was designed to evaluate the circulation of group a rotaviruses in french hospitalized children, and to detect unusual strains. this prospective study was conducted from 2001 to 2006 in children consulting for acute diarrhea at the pediatric emergency department in three french university hospitals. the rotaviruses were detected by rapid test and genotyped by rt-pcr on the basis of their outer capsid proteins vp4 (p-type) and vp7 (g-type). the stools from 757 children were analyzed. g1 ... | 2009 | 18855026 |
| [rotavirus vaccine]. | rotavirus gastroenteritis is one of the most important diseases in young children. there are 400,000 to 600,000 deaths annually among children under 5 years old in the world, especially in developing countries. rotavirus vaccine has been developed since 1980s. however vaccine development and trial took a long time before the commercial available. one reason is the genetic variability of rotavirus, there are more than 10 serotypes (g types) in group a rotavirus. another reason is side reaction su ... | 2008 | 18939500 |
| supplementing suckling rats with whey protein concentrate modulates the immune response and ameliorates rat rotavirus-induced diarrhea. | group a rotaviruses (rv) are the most common causative agents of acute gastroenteritis in children <2 y. the present study was designed to establish the effect of a bovine whey protein concentrate (wpc) in a rv infection model in suckling rats. from d 3 of life, suckling lewis rats received daily supplements of wpc, wpc plus lactoferrin (lf), standard infant formula (sif), or water (rv-infected group and an untreated, uninfected reference group). on d 8 of life, heterologous simian rv sa-11 was ... | 2008 | 19022963 |
| [evaluation of two immunochromatography kits for rapid diagnosis of rotavirus infections]. | a prospective study was conducted to evaluate two immunochromatography (icg) commercial kits for diagnosis of rotavirus infection, vikia rota-adeno (biomérieux) and simple rota-adeno (operon). reverse transcriptase and polymerase chain reaction (rt-pcr) with specific primers for the vp7 gene of group a rotavirus was used as the reference method. the sensitivity and specificity of the icg tests compared with those of the reference method were 98.4% and 84.8%, respectively, for simple rota-adeno ( ... | 2008 | 19024505 |
| molecular characterization of vp4, vp6, vp7, nsp4, and nsp5/6 genes identifies an unusual g3p[10] human rotavirus strain. | an unusual strain of human rotavirus g3p[10] (cmh079/05) was detected in a stool sample of a 2-year-old child admitted to the hospital with severe diarrhea in chiang mai, thailand. analysis of the vp7 gene sequence revealed highest identities with unusual human rotavirus g3 strain cmh222 at 98.7% on the nucleotide and 99.6% on the amino acid levels. phylogenetic analysis of the vp7 sequence confirmed that the cmh079/05 strain formed a cluster with g3 rotavirus reference strains and showed the cl ... | 2009 | 19031442 |
| analysis of the vp6 gene of human and porcine group a rotavirus strains with unusual subgroup specificities. | full-length vp6 amino acid sequences of human and porcine rotaviruses with subgroup (sg) (i + ii) and sg non-(i + ii) were analyzed in comparison with those of sg i and sg ii. in human rotaviruses, the strains in the same sg shared a very high degree of amino acid identity, ranging from 97.4% to 99.4% for sg i, 95.9% to 100% for sg ii, and 99.4% to 100% for sg non-(i + ii), while viruses in different sgs shared somewhat lower sequence identity at 90.4-93.1%. conserved amino acids that distinguis ... | 2009 | 19031468 |
| molecular characterization of rotaviruses, noroviruses, sapovirus, and adenoviruses in patients with acute gastroenteritis in thailand. | outbreaks of viral gastroenteritis occur worldwide including thailand. unfortunately, there is limited information since etiologic agents have not been identified in several outbreaks of nonbacterial gastroenteritis. the genotype of enteric viruses causing acute gastroenteritis in thailand was determined using reverse transcription-multiplex polymerase chain reaction and dna sequencing. from january 2006 to february 2007, stool samples were collected from patients with acute gastroenteritis of a ... | 2009 | 19107961 |
| phylogenetic analysis of rotaviruses with predominant g3 and emerging g9 genotypes from adults and children in wuhan, china. | prevalence and phylogenetic relatedness of rotaviruses causing diarrheal diseases in children and adults were analyzed in wuhan, china. during a period between june 2006 and february 2008, group a rotavirus was identified in 24.9% (280/1126) and 7.6% (83/1088) of specimens taken from children and adults, respectively. g3p[8] was the most frequent genotype in both children (66.3%) and adults (62.7%), followed by g1p[8] (20.3% and 26.2%, respectively). g9 was detected in specimens from six childre ... | 2009 | 19107964 |
| characterization of novel vp7, vp4, and vp6 genotypes of a previously untypeable group a rotavirus. | rotavirus is the most common cause of acute gastroenteritis among infants and young children throughout the world, but rotavirus cases in developing countries account for nearly all of the approximately 600,000 annual deaths. we studied the epidemiology of rotavirus in 22 rural communities in northern coastal ecuador over a five-year period. from 250 rotavirus positive stool specimens, the percentage that could not be rt-pcr genotyped for vp4 and vp7 was 77% and 63%, respectively. the possibilit ... | 2009 | 19131083 |
| intranasal immunization of mice with vp2 dna of human rotavirus a induces cellular and humoral immunity. | dna vaccination using a plasmid encoding human rotavirus a (hurv-a) inner capsid vp2 was examined in a mouse model. balb/c mice were immunized intranasally (i.n.) with a vp2 dna vaccine that induced cellular and humoral immune response to hurv-a. the increased levels of cytokines ifn-gamma and il-4 and the production of anti-vp2 igg antibodies were detected. in addition, splenocyte proliferation detected by mtt test was enhanced in the mice treated with the vaccine. these results may encourage t ... | 2008 | 19143478 |
| group a rotavirus genotypes and the ongoing brazilian experience: a review. | brazil was the first latin american country to introduce universal group a rotavirus (rv-a) vaccination in march 2006, resulting in a unique epidemiological scenario. since rv-a first identification in brazil, 2,691 rv-a-positive stool samples, collected between 1982- 2007, were typed by independent research groups throughout the country. in the pre-vaccination era, 2,492 rv-a-positive samples collected from 1982-2005 were successfully typed, while 199 samples were analyzed from 2006-2007. accor ... | 2008 | 19148411 |
| [expression and immunogenicity analysis of recombinant plasmid pw425et-vp7 of porcine rotavirus a in lactobacillus]. | we tested the antigenicity of porcine rotavirus vp7 gene expressed in lactobacillus. | 2008 | 19149168 |
| seasonal shifts of group a rotavirus strains as a possible mechanism of persistence in the human population. | this article demonstrates how the seasonal predominance of a new rotavirus strain in asuncion, paraguay is correlated with a wide spectrum of age groups of children infected in that given season. therefore, this study provides new evidence to support the idea that seasonal shift of rotavirus strains is a possible mechanism used by the virus to evade herd immunity (acquired by the population due to previous infections) and, thus, ultimately persist in that population. | 2009 | 19152398 |
| [nucleotide sequence analysis of the nsp4 gene from group a rotaviruses isolated in nizhni novgorod]. | the complete nucleotide sequences of the nsp4 gene from russian isolates of group a rotaviruses with different g[p] types obtained in nizhni novgorod have been first ascertained. they belong to their affiliation to three nsp4 (a, b, and c) of the 5 known genotypes. eleven strains had nsp4 genotype. phylogenetic analysis based on the complete nucleotide sequences of the nsp4 gene revealed the sequence homology of g1p[8] viruses with the strains circulating in scandinavia and that of the g3 type v ... | 2008 | 19172905 |
| diagnosing rotavirus a associated iid: using elisa to identify a cut-off for real time rt-pcr. | the use of rt-pcr for diagnosis of group a rotaviruses is increasing, but up to 14% of healthy individuals may be positive by rt-pcr. if rt-pcr is not well correlated with disease, rotavirus a may not always be the cause of illness in rt-pcr positive patients with infectious intestinal disease (iid). | 2009 | 19179107 |
| viral agents associated with acute gastroenteritis in children hospitalized with diarrhea in lanzhou, china. | gastroenteritis is a major cause of childhood morbidity and mortality worldwide. rotavirus, human caliciviruses (hucv), adenovirus, and astrovirus are recognized as common etiologies of acute gastroenteritis. | 2009 | 19201258 |
| assignment of the group a rotavirus nsp4 gene into genotypes using a hemi-nested multiplex pcr assay: a rapid and reproducible assay for strain surveillance studies. | the rotavirus non-structural protein nsp4 has been implicated in a number of biological functions during the rotavirus cellular cycle and pathogenesis, and has been addressed as a target for vaccine development. the nsp4 gene has been classified into six genotypes (a-f). a semi-nested triplex pcr was developed for genotyping the major human nsp4 genotypes (a-c), which are common in human rotavirus strains but are also shared among most mammalian rotavirus strains. a total of 192 previously chara ... | 2009 | 19208878 |
| the first complete genome sequence of a chicken group a rotavirus indicates independent evolution of mammalian and avian strains. | group a rotaviruses are a leading cause of gastroenteritis in humans and animals. transmission between mammalian species and humans has been demonstrated repeatedly. here, the first entire genome sequence (19,064 bp) of a chicken rotavirus, strain ch-02v0002g3, is presented. a low degree of nucleotide sequence identity with the mammalian group a rotaviruses is evident for all 11 genome segments, whereas a closer relationship to available rotavirus sequences from avian species has been determined ... | 2009 | 19246068 |
| evidence of interspecies transmission and reassortment among avian group a rotaviruses. | avian rotaviruses are broadly distributed among birds, but only scarcely characterized on the molecular level. the vp4-, vp6-, vp7- and nsp5-encoding sequences of eight group a rotaviruses from chickens and turkeys determined here indicate a low degree of sequence similarity with mammalian rotaviruses. an nsp6-encoding region was missing in all chicken isolates except for isolate ch2. four novel genotypes (p[30], p[31], g22 and h8) were assigned by the rotavirus classification working group. gen ... | 2009 | 19249805 |
| first molecular detection of group a rotaviruses in drinking water sources in beijing, china. | the most prevalent group a rotavirus found in the diarrheic children was also determined in drinking water sources including raw water, treated water and tap water in beijing, and then the possible contamination contributions to tap water for human consumption were discussed in this study. a total of 26 raw water samples, 77 treated water samples and 143 tap water samples in beijing were collected for analysis of group a rotavirus from april 2006 to august 2007. according to the results, it was ... | 2009 | 19330275 |
| full genomic analysis of a human group a rotavirus g9p[6] strain from eastern india provides evidence for porcine-to-human interspecies transmission. | deduced amino acid sequence and phylogenetic analyses of a group a rotavirus g9p[6] strain (designated as mcs/13-07), detected from a 3-year-old child in eastern india, revealed a vp8* closely related to porcine p[6] strains (p[6] sublineage 1d), and the vp7 clustered with g9 lineage-iii strains. to our knowledge, this is the first report of human p[6] strain clustering in sublineage id. thus, to further characterize the evolutionary diversity of strain mcs/13-07, all gene segments were analyzed ... | 2009 | 19333549 |
| detection of human viruses in rivers of a densly-populated area in germany using a virus adsorption elution method optimized for pcr analyses. | transmission of viruses via surface water is a major public health concern. to determine the viral concentration in rivers of a densely-populated area in germany, the virus adsorption elution (viradel) method was optimized for downstream pcr applications. using a high-salt alkaline phosphate buffer for elution, the median recovery efficiency from spiked 1l water samples ranged from 21.3% to 100% for jc polyomavirus, human adenovirus type 5, echovirus 11, and norovirus genogroup i. analyses of 41 ... | 2009 | 19361832 |
| a study on the possibility of zoonotic infection in rotaviral diarrhoea among calves and buffalo calves in and around kolkata, india. | group a and group b rotaviruses are important diarrhea causing agents among calves and buffalo calves. epidemiological studies in indian calves revealed the predominance of group a rotavirus strains with g6, g8, and g10 specificity and group b rotaviruses. a total of 95 fecal samples were collected from calves and buffalo calves affected with diarrhea from an unorganized cattle farm and two cattle markets in and around kolkata, in the state of west bengal of eastern india. rotaviruses were detec ... | 2009 | 19364081 |
| detection of caliciviruses associated with acute infantile gastroenteritis in salvador, an urban center in northeast brazil. | acute gastroenteritis caused by viruses is one of the leading causes of infantile morbidity. the aim of the present study was to investigate the presence of human caliciviruses of the genera norovirus and sapovirus in children up to 3 years of age with acute gastroenteritis from low-income communities in the city of salvador, brazil. this study is an extension of previous work carried out to establish the profile of the most prevalent enteric pathogens present in these communities. in this repor ... | 2009 | 19377793 |
| detection of group c rotavirus in juvenile ferrets (mustela putorius furo) with diarrhea by reverse transcription polymerase chain reaction: sequencing and analysis of the complete coding region of the vp6 gene. | nine juvenile ferrets (mustela putorius furo) with a history of diarrhea were severely dehydrated and had distended abdomens and thin-walled small intestines that contained gas and fluid. histologically, small intestines exhibited acute superficial atrophic enteritis. transmission electron microscopy of the small intestine showed rotavirus-like particles within apical vacuoles. reverse transcription polymerase chain reaction (rt-pcr) was negative for group a rotavirus. a group c rotavirus-specif ... | 2009 | 19429992 |
| genomic diversity of group a rotavirus strains in patients aged 1-36 months admitted for acute watery diarrhoea in northern india: a hospital-based study. | the diversity and capacity of human rotaviruses for rapid evolution and genetic reassortment suggests that rotavirus vaccine should be designed to provide heterotypic protection. the objective of the present study was to provide information on the circulating genotypes of rotavirus and allied baseline epidemiology in lucknow, india. in a cross-sectional study, the prevalence of rotavirus g-p types was studied in patients aged 1-36 months with acute watery diarrhoea. various sociodemographic, env ... | 2010 | 19456832 |
| molecular epidemiology of rotaviruses among healthy calves in japan: isolation of a novel bovine rotavirus bearing new p and g genotypes. | a total of 171 fecal specimens collected from healthy calves on a beef farm in gifu prefecture, japan in 2006-2007 were examined for group a rotaviruses by rt-semi-nested pcr targeting the coding region for vp8*. nine specimens were positive for rotavirus. g and p genotyping indicated that one strain was g10p[11]-like and six strains were considered to be the same unknown g and p genotypes. among these six untypeable strains, one strain, azuk-1, was adapted to cell culture and analyzed. sequence ... | 2009 | 19464329 |
| multiplex pcr and multiplex rt-pcr for inclusive detection of major swine dna and rna viruses in pigs with multiple infections. | multiplex pcr and multiplex rt-pcr were developed to identify nine viruses in pigs with multiple infections. these viruses are: porcine circovirus type 2 (pcv2), suid herpesvirus 1, porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus, porcine rotavirus a (porv-a), porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and getah virus. these methods were shown to be high specificity and sensitivity. in the ... | 2009 | 19467264 |
| relationship between electropherotypes and vp7/vp4 genotypes of group a rotaviruses detected between 2000 and 2007 in tunisian children. | background: rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. analysis of mobility of the 11 segments of genomic rna by polyacrylamide gel electrophoresis (page) yields a pattern which is characteristic for a particular rotavirus isolate. the group a rotaviruses can be further characterized by analysis of vp7 and vp4 genes specificities, responsible for rotavirus classification into g and p genotypes, respectively. the aim of the present study was to detec ... | 2009 | 19481882 |
| [norovirus infection in general hospitals for adults]. | to determine the role of noroviruses in the rise of hospital outbreaks of acute intestinal infections (aii), to study clinical and epidemiological aspects of hospital noroviral infection. | 2009 | 19514424 |
| [rapid detection of rotavirus in water samples using immunomagnetic separation combined with real time pcr]. | a quantitative and rapid detection method for rotavirus in water samples was developed, by using immunomagnetic separation combined with reverse transcription and real time polymerase chain reaction (ims-rt-real time pcr). magnetic beads coated with antibodies directed against group a rotavirus were used to capture and purify the virus in water samples. the experimental results showed that ims was optimized when 1 ml samples were supplemented with 10 microl of immunomagnetic beads, 2.5 microl of ... | 2009 | 19558103 |
| genotypification of bovine group a rotavirus in méxico. | bovine scours, frequently provoked by rotavirus infection, causes significant economic losses. nevertheless, no information exists about the bovine rotavirus genotypes present in mexico. this information is necessary for designing efficient vaccines. in this work, 128 samples from diarrheic calves were collected between 2005 and 2006 in 26 dairy and/or beef cattle herds located in 10 regions of mexico, and analyzed for the presence of group a rotavirus. g and p genotypes were determined by pcr i ... | 2009 | 19559115 |
| molecular analysis of g3 rotavirus among infants and children in dhaka city, bangladesh after 1993. | between 2004 and 2005, 917 fecal specimens were collected from children below age 5 who presented to child health institute for treatment of diarrhoea in dhaka city, bangladesh. the specimens were screened by rt-pcr for the presence of group a rotavirus and positive stools genotyped. group a rotavirus was detected in 307 stools and serotype g3p[8] strains were detected in nine specimens. sequence analysis clustered the g3 strains into one distinct lineage (lineage i) with other asian g3 strains. ... | 2009 | 19560559 |
| rotavirus infections in galapagos sea lions. | group a rotaviruses infect and cause diarrhea in the young of a broad range of terrestrial mammals, but it is unknown, to our knowledge, whether they infect marine mammals. during february and march of 2002 and 2003, we collected 125 serum samples and 18 rectal swab samples from galapagos sea lion pups (gsl, zalophus wollebaeki), and 22 serum samples from galapagos fur seal pups (gfs, arctocephalus galapagoensis) from nine islands of the galapagos archipelago, ecuador. sera were tested for antib ... | 2009 | 19617482 |
| prevalence of vp7 and vp4 genotypes of human group a rotavirus in infants and children with acute diarrhea in a northern city of bangladesh. | the objective of this cross sectional prospective study was to determine rotavirus etiology and prevalence of the different rotavirus genotypes causing acute gastroenteritis in infants and children (</=5years) in mymensingh medical college hospital, bangladesh. the study was conducted during the period from january 2005 to june 2006. a total of 962 stool specimens from diarrheic children were analyzed by polyacrylamide gel electrophoresis (page). rotavirus detection rate was 31.3% (301/962). amo ... | 2009 | 19623146 |
| electropherotypes and subgroups of group a rotaviruses circulating among diarrhoeic children in kano, nigeria. | it is estimated that about 600,000 children die annually as a result of severe dehydrating diarrhea caused by rotaviruses. the virus is a double stranded rna (dsrna) virus with 11 segments. group a rotaviruses show a characteristic 4-2-3-2 pattern following electrophoresis. the vp6 subgroups, i and ii exist. this work was carried out to study the prevalence of rotavirus infection among children 0-5 years with diarrhea in kano, and to determine the circulating subgroups and electropherotypes and ... | 2008 | 19623917 |
| epidemiology of rotavirus and astrovirus infections in children in northwestern nigeria. | recent estimates attribute 527,000 deaths in children less than five years of age to rotavirus diarrhea annually, with 145,000 occurring in sub-saharan africa. human astroviruses have been identified as one of the most frequent causes of infantile diarrhea, second in incidence only to rotavirus. this study was conducted to determine the prevalence of rotavirus and astrovirus and also to establish the circulating strains of rotavirus in a community in nigeria where most diarrheic patients do not ... | 2008 | 19623918 |
| detection of rotavirus rna and antigens in serum and cerebrospinal fluid samples from diarrheic children with seizures. | group a rotavirus (garv) genes (the vp7 and nsp3 genes) in acute-phase cerebrospinal fluid (csf), sera and stool samples from 6 children with convulsions accompanied by garv gastroenteritis were investigated by reverse transcription-polymerase chain reaction (rt-pcr). when the vp7 gene was amplified from the samples, the g genotype (g type) of garv was determined by rt-pcr. garv genes were detected in the csf samples of all 6 children, in 2 of the 3 blood samples, and in all of 4 stool samples. ... | 2009 | 19628905 |
| national rotavirus surveillance in argentina: high incidence of g9p[8] strains and detection of g4p[6] strains with porcine characteristics. | group a rotaviruses are the most frequently detected viral agents associated with diarrhea in infants and children worldwide. it has been estimated that every year almost 120,000 cases of diarrhea associated with rotavirus occur in children under 5 years old in argentina. in this work, we present the rotavirus strain diversity detected during the first 2 years of the national surveillance network for diarrheas implemented by the ministry of health in argentina. during 2006 and 2007 a total of 46 ... | 2009 | 19631763 |
| environmental dissemination of group a rotavirus: p-type, g-type and subgroup characterization. | rotaviruses a (rv-a) infection is the most common cause of acute diarrheal diseases in infants and the dissemination of these viruses in the environment represents a public health hazard. the present study aims to evaluate reverse transcription-polymerase chain reaction (rt-pcr) based protocols for the detection of rv-a genes in different types of environmental samples. rv-a were concentrated by the adsorption-elution method using negatively charged membranes associated with a centriprep concent ... | 2009 | 19657158 |
| detection and characterization of group a rotaviruses in children hospitalized with acute gastroenteritis in norway, 2006-2008. | to assess the genetic diversity of rotavirus strains in norway, the distribution of rotavirus genotypes was studied in children admitted to hospital with acute gastroenteritis. the detection of rotavirus in stool samples was compared using an enzyme-linked immunosorbent assay (elisa), an immunochromatographic test and rt-pcr. children <5 years of age admitted to hospital with diarrhea in three large hospitals were enrolled prospectively from march 2006 to february 2008. rotavirus was detected in ... | 2009 | 19697411 |
| evolutionary consequences of g9 rotaviruses circulating in thailand. | infection with group a rotaviruses is the main cause of acute gastroenteritis in infants and young children worldwide. rotavirus g9 is recognized as the fifth most predominant g genotype that spreads throughout the world. in this study, we describe the changing distribution of rotavirus g9 genotype in chiang mai, thailand, from 1989 to 2005. molecular genetic evolutionary analysis of the g9 rotavirus vp7 gene was performed. the g9 rotavirus in thailand was first detected in chiang mai in 1989 wi ... | 2009 | 19716437 |
| severity of acute gastroenteritis in infants infected by g1 or g9 rotaviruses. | group a rotaviruses are the main viral causative agent of acute diarrhea, and cause considerable morbidity in children. g9 rotaviruses have recently emerged all over the world and are thought to give more severe symptoms because of a lack of previous exposure and the absence of maternal antibodies in patients. | 2009 | 19726224 |
| viral gastroenteritis and diversity of rotavirus strains in colombian children: a systematic review. | to determine the epidemiological profile of viruses associated with acute gastroenteritis in children younger than 5 years of age and the distribution of rotavirus genotypes circulating in colombia. | 2008 | 19738332 |
| in vitro antiherpetic and antirotaviral activities of a sulfate prepared from mimosa scabrella galactomannan. | a chemically sulfated galactomannan (brs) from seeds of mimosa scabrella had in vitro antiviral activity against herpes simplex virus 1 (hsv-1), but not against simian rotavirus a/sa11 (sirv-a/sa11). it was examined by (13)c nmr spectroscopy, which showed the sulfate groups to be mainly at c-6 of galactose residues. brs had a selective inhibition against hsv-1 during its attachment step, having an ic(50) lower than 2.5microg/ml, determined by plaque reduction, and a selectivity index of greater ... | 2009 | 19751759 |
| enteropathogens and risk factors for diarrhea in norwegian dairy calves. | the aims of the current study were to estimate the prevalence of enteropathogens in calves in norwegian dairy herds, evaluate the clinical consequences of protozoal infections, and identify risk factors for diarrhea. the 135 participating herds were randomly selected from those in the norwegian dairy herd recording system that had at least 15 cow-years. each herd was followed for 1 yr. fecal samples from calves with (n = 68) or without (n = 691) diarrhea were analyzed for the presence of cryptos ... | 2009 | 19762824 |
| [rapid detection of norwalk gii with reverse transcription loop-mediated isothermal amplification]. | to develop a reverse transcription-loop-mediated isothermal amplification (rt-lamp) assay for rapid and sensitive detection of norwalk gii. 4 primers which recognized 6 distinct regions on the rna-dependent rna polymerase gene of norwalk gii were designed and used for lamp assay. norwalk gii rna was amplified under isothermal conditions (65 degrees c) for 120 min, and lamp results were then judged with naked eye, sybr green i staining, electrophoretic analysis and restriction digestion. to evalu ... | 2009 | 19769163 |
| prevalence of diarrhea viruses in hospitalized children in hong kong in 2008. | a total of 209 stool samples were collected from pediatric patients admitted for acute gastroenteritis in a hospital in hong kong, during an 8-month period from january to august 2008, and were tested for the presence of rotavirus, norovirus, sapovirus, adenovirus, and astrovirus using a multiplex rt-pcr assay. the most common virus was rotavirus group a (59 of 209, 28%, mainly serotypes g1, g2, g3, and g9), followed by norovirus group ii (48 of 209, 23%), adenovirus (7 of 209, 3%, serotypes 2, ... | 2009 | 19774686 |
| enteric calicivirus and rotavirus infections in domestic pigs. | we report the prevalence of rotavirus and calicivirus infections, along with their respective association with diarrhoea in the porcine population of the region of northern spain. a total of 221 samples were collected at random from different farms in the region and from the main slaughterhouse facility in the city of zaragoza. faecal samples were scored as diarrhoeic or normal and grouped into five groups to match general farm management and age criteria: group i (suckling 0-4 weeks), group ii ... | 2010 | 19781118 |
| zoonotic aspects of rotaviruses. | rotaviruses are important enteric pathogens of humans and animals. group a rotaviruses (garvs) account for up to 1 million children deaths each year, chiefly in developing countries and human vaccines are now available in many countries. rotavirus-associated enteritis is a major problem in livestock animals, notably in young calves and piglets. early in the epidemiological garv studies in humans, either sporadic cases or epidemics by atypical, animal-like garv strains were described. complete ge ... | 2010 | 19781872 |
| human infection with g12 rotaviruses, germany. | rotavirus group a g12 genotypes were detected in 3 (1.5%) of 198 stool samples positive for human rotavirus. g12p[6] was present in 2 samples, and a mixed g3g12p[8] was found in 1 sample. phylogenetic analysis of complete open reading frames of all 11 genomic rna segments proved their wa-like genogroup affiliation. | 2009 | 19788829 |
| prevalence of rotavirus in acute diarrhea and its association with clinical signs and symptoms. | rotaviruses are the main etiological agents of acute infectious diarrhea in children. thus, the objective of this study is to contribute to the information about the rotavirus variants circulating in brazil. a cross-sectional study was conducted on 124 fecal samples collected from children up to the age of 5 years, with acute gastroenteritis. thirty-one samples were positive for rotavirus a. regarding the g genotyping, 16 (80%) were classified as g1 and regarding p genotyping all strains were cl ... | 2010 | 19793895 |