Publications

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vision-mediated exploitation of a novel host plant by a tephritid fruit fly.shortly after its introduction into the hawaiian islands around 1895, the polyphagous, invasive fruit fly bactrocera (zeugodacus) cucurbitae (coquillett) (diptera: tephritidae) was provided the opportunity to expand its host range to include a novel host, papaya (carica papaya). it has been documented that female b. cucurbitae rely strongly on vision to locate host fruit. given that the papaya fruit is visually conspicuous in the papaya agro-ecosystem, we hypothesized that female b. cucurbitae u ...201728380069
combining cue-lure and methyl eugenol in traps significantly decreases catches of most bactrocera, zeugodacus and dacus species (diptera: tephritidae: dacinae) in australia and papua new guinea.male fruit fly attractants, cue-lure (cl) and methyl eugenol (me), are important in the monitoring and control of pest fruit fly species. species respond to cl or me but not both, and there are conflicting reports on whether combining cl (or its hydroxy analogue raspberry ketone) and me decreases their attractiveness to different species. fruit fly monitoring programs expend significant effort using separate cl and me traps and avoiding lure cross-contamination, and combining the two lures in on ...201729272411
a chromosome-scale assembly of the bactrocera cucurbitae genome provides insight to the genetic basis of white pupae.genetic sexing strains (gss) used in sterile insect technique (sit) programs are textbook examples of how classical mendelian genetics can be directly implemented in the management of agricultural insect pests. although the foundation of traditionally developed gss are single locus, autosomal recessive traits, their genetic basis are largely unknown. with the advent of modern genomic techniques, the genetic basis of sexing traits in gss can now be further investigated. this study is the first of ...201728450369
insecticidal activity of the leaf essential oil of peperomia borbonensis miq. (piperaceae) and its major components against the melon fly bactrocera cucurbitae (diptera: tephritidae).the essential oil from the leaves of peperomia borbonensis from réunion island was obtained by hydrodistillation and characterized using gc-fid, gc/ms and nmr. the main components were myristicin (39.5%) and elemicin (26.6%). the essential oil (eo) of peperomia borbonensis and its major compounds (myristicin and elemicin), pure or in a mixture, were evaluated for their insecticidal activity against bactrocera cucurbitae (diptera: tephritidae) using a filter paper impregnated bioassay. the concen ...201728273402
incongruence between molecules and morphology: a seven-gene phylogeny of dacini fruit flies paves the way for reclassification (diptera: tephritidae).molecular and morphological research often suggest conflicting results. selective pressure on certain morphologies can confound understanding of evolutionary relationships. dacini is one of the most diverse tribes of tephritid flies and contains many economically important pest species. their black and yellow patterned body markings are presumed to act as wasp mimicry, and the characters separating species and groups are limited and in some cases phenotypically plastic. the traditional taxonomy ...201729224785
isolation and molecular characterization of the transformer gene from bactrocera cucurbitae (diptera: tephritidae).transformer (tra) is a switch gene of sex determination in many insects, particularly in dipterans. however, the sex determination pathway in bactrocera cucurbitae (coquillett), a very destructive pest on earth, remains largely uncharacterized. in this study, we have isolated and characterized one female-specific and two male-specific transcripts of the tra gene (bcutra) of b. cucurbitae. the genomic structure of bcutra has been determined and the presence of multiple conserved transformer (tra) ...201728931159
identification of the ubiquitous antioxidant tripeptide glutathione as a fruit fly semiochemical.many insects mark their oviposition sites with a host marking pheromone (hmp) to deter other females from overexploiting these sites. previous studies have identified and used hmps to manage certain fruit fly species; however, few are known for african indigenous fruit flies. the hmp of the african fruit fly, ceratitis cosyra, was identified as the ubiquitous plant and animal antioxidant tripeptide, glutathione (gsh). gsh was isolated from the aqueous extract of adult female fecal matter and cha ...201728911226
chemical degradation of tmr multilure dispensers for fruit fly detection weathered under california climatic conditions.degradation models for multilure fruit fly trap dispensers were analyzed to determine their potential for use in large california detection programs. solid three-component male lure tmr (trimedlure [tml], methyl eugenol [me], raspberry ketone [rk]) dispensers impregnated with ddvp (2, 2-dichlorovinyl dimethyl phosphate) insecticide placed inside jackson traps were weathered during summer (8 wk) and winter (12 wk) in five citrus-growing areas. additionally, tmr wafers without ddvp, but with an in ...201728854647
using two-sex life tables to determine fitness parameters of four bactrocera species (diptera: tephritidae) reared on a semi-artificial diet.fruit flies in the genus bactrocera are global, economically important pests of agricultural food crops. however, basic life history information about these pests, which is vital for designing more effective control methods, is currently lacking. artificial diets can be used as a suitable replacement for natural host plants for rearing fruit flies under laboratory conditions, and this study reports on the two-sex life-table parameters of four bactrocera species (bactrocera correcta, bactrocera d ...201728944746
population susceptibility to insecticides and the development of resistance in bactrocera cucurbitae (diptera: tephritidae).excessive insecticide applications are commonly used to manage bactrocera cucurbitae coquillett in china. resistance status, resistance development trends, and patterns of cross-resistance to insecticides in b. cucurbitae were investigated. among 21 populations from hainan island, two populations expressed high resistance to beta-cypermethrin; seven, eight, and ten populations expressed intermediate resistance to spinosad, avermectin, and beta-cypermethrin, respectively; four, six, one, five, an ...201626668351
identification and preliminary characterization of chemosensory perception-associated proteins in the melon fly bactrocera cucurbitae using rna-seq.an investigation into proteins involved in chemosensory perception in the melon fly, bactrocera cucurbitae (diptera: tephritidae) is described here using a newly generated transcriptome dataset. the melon fly is a major agricultural pest, widely distributed in the asia-pacific region and some parts of africa. for this study, a transcriptome dataset was generated using rna extracted from 4-day-old adult specimens of the melon fly. the dataset was assembled and annotated via gene ontology (go) ana ...201626752702
captures of wild ceratitis capitata, bactrocera dorsalis, and bactrocera cucurbitae (diptera: tephritidae) in traps with improved multilure tmr dispensers weathered in california.during 2012–2013, solid mallet tmr (trimedlure [tml], methyl eugenol [me], raspberry ketone [rk]) wafers impregnated with ddvp (2, 2-dichlorovinyl dimethyl phosphate) insecticide were weathered during summer (8 wk) and winter (12 wk) in five california citrus-growing counties (kern, ventura, orange, tulare, and riverside). in addition, tmr wafers without ddvp and with a hercon vaportape ii insecticidal strip were compared with tmr dispensers with ddvp at exeter and riverside. weathered treatment ...201626582906
diversity of bacterial communities in the midgut of bactrocera cucurbitae (diptera: tephritidae) populations and their potential use as attractants.the microbiota plays an important role in insect development and fitness. understanding the gut microbiota composition is essential for the development of pest management strategies. midgut bacteria were isolated from nine wild b. cucurbitae populations collected from different agroecological zones of india. these isolates were further studied for attractant potential of fruit fly adults, and the chemical constituents in the supernatants of gut bacteria were analysed.201626299539
relative tolerance of six bactrocera (diptera: tephritidae) species to phytosanitary cold treatment.to compare relative cold treatment tolerance across the economically important tephritid fruit flies (diptera: tephritidae), bactrocera carambolae drew & hancock, bactrocera correcta (bezzi), bactrocera cucurbitae (coquillett), four populations of bactrocera dorsalis (hendel), bactrocera zonata (saunders), and bactrocera tryoni (froggatt), eggs (in vitro), and larvae (in infested fruit or on carrot diet) were cold treated at 2.0 ± 0.2 °c for selected durations. the study was performed to assess ...201627660425
host plant range of a fruit fly community (diptera: tephritidae): does fruit composition influence larval performance?phytophagous insects differ in their degree of specialisation on host plants, and range from strictly monophagous species that can develop on only one host plant to extremely polyphagous species that can develop on hundreds of plant species in many families. nutritional compounds in host fruits affect several larval traits that may be related to adult fitness. in this study, we determined the relationship between fruit nutrient composition and the degree of host specialisation of seven of the ei ...201627650549
characterization of irritans mariner-like elements in the olive fruit fly bactrocera oleae (diptera: tephritidae): evolutionary implications.genomic variation among species is commonly driven by transposable element (te) invasion; thus, the pattern of tes in a genome allows drawing an evolutionary history of the studied species. this paper reports in vitro and in silico detection and characterization of irritans mariner-like elements (mles) in the genome and transcriptome of bactrocera oleae (rossi) (diptera: tephritidae). eleven irritans mle sequences have been isolated in vitro using terminal inverted repeats (tirs) as primers, and ...201627392643
assessing the risk of invasion by tephritid fruit flies: intraspecific divergence matters.widely distributed species often show strong phylogeographic structure, with lineages potentially adapted to different biotic and abiotic conditions. the success of an invasion process may thus depend on the intraspecific identity of the introduced propagules. however, pest risk analyses are usually performed without accounting for intraspecific diversity. in this study, we developed bioclimatic models using maxent and boosted regression trees approaches, to predict the potential distribution in ...201526274582
novel toxicity of bacillus thuringiensis strains against the melon fruit fly, bactrocera cucurbitae (diptera: tephritidae).bactrocera cucurbitae (melon fruit fly) is one of the most detrimental vegetable-damaging pests in bangladesh. the toxicity of bacillus thuringiensis (bt) has been reported against a few genera of bactrocera in addition to numerous other insect species. bt strains, harbouring cry1a-type genes were, therefore, assayed in vivo against the 3(rd) instar larvae of b. cucurbitae in this study. the biotype-based prevalence of cry1 and cry1a genes was calculated to be 30.8% and 11.16%, respectively, of ...201526133509
field evaluation of melolure, a formate analogue of cuelure, and reassessment of fruit fly species trapped in sydney, new south wales, australia.in australia, tephritids are usually attracted to either cuelure or methyl eugenol. methyl eugenol is a very effective lure, but cuelure is less effective likely due to low volatility. a new formate analogue of cuelure, melolure, has increased volatility, resulting in improved efficacy with the melon fruit fly, bactrocera cucurbitae coquillett. we tested the efficacy of melolure with fruit fly species in sydney as part of the national exotic fruit fly monitoring programme. this monitoring progra ...201526470243
reconstructing a comprehensive transcriptome assembly of a white-pupal translocated strain of the pest fruit fly bactrocera cucurbitae.bactrocera cucurbitae is a serious global agricultural pest. basic genomic information is lacking for this species, and this would be useful to inform methods of control, damage mitigation, and eradication efforts. here, we have sequenced, assembled, and annotated a comprehensive transcriptome for a mass-rearing sexing strain of this species. this forms a foundational genomic and transcriptomic resource that can be used to better understand the physiology and biochemistry of this insect as well ...201525830018
weathering and chemical degradation of methyl eugenol and raspberry ketone solid dispensers for detection, monitoring, and male annihilation of bactrocera dorsalis and bactrocera cucurbitae (diptera: tephritidae) in hawaii.solid male lure dispensers containing methyl eugenol (me) and raspberry ketone (rk), or mixtures of the lures (me + rk), and dimethyl dichloro-vinyl phosphate (ddvp) were evaluated in area-wide pest management bucket or jackson traps in commercial papaya (carica papaya l.) orchards where both oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett), are pests. captures of b. dorsalis with fresh wafers in jackson and bucket traps were significantly highe ...201526470301
assessment of navel oranges, clementine tangerines, and rutaceous fruits as hosts of bactrocera cucurbitae and bactrocera latifrons (diptera: tephritidae).export of citrus spp. fruits may require risk mitigation measures if grown in areas with established tephritid fruit fly (diptera: tephritidae) populations capable of infesting the fruits. the host status of citrus spp. fruits is unclear for two tephritid fruit fly species whose geographic ranges have expanded in recent years: melon fly, bactrocera cucurbitae (cocquillett), and bactrocera latifrons (hendel). in no choice cage infestation studies, b. latifrons oviposited into intact and punctured ...201526816484
developmental biology of zeugodacus cucurbitae (diptera: tephritidae) in three cucurbitaceous hosts at different temperature regimes.fruit flies are key pests of cucurbits in many parts of the world, including tanzania. developmental biology of zeugodacus cucurbitae (coquillett) has been determined across temperature regimes in some cucurbitaceous hosts, in limited geographies. this study was conducted to determine duration and survival rates of immature stages of z. cucurbitae in three cucurbitaceous hosts, at different temperature regimes. it was hypothesized that temperature and cucurbitaceous hosts influence duration and ...201526589874
disruption of darna pallivitta (lepidoptera: limacodidae) by conventional and mobile pheromone deployment.identification of the darna pallivitta (moore) pheromone component n-butyl (e)-7,9-decadienoate (e7,9-10:coon-bu) has made it possible to investigate communication disruption to control this lepidopteran pest. conventional communication disruption trials showed marked decreases in the mean number of male moths captured in e7,9-10:coonbu-treated fields compared with control fields. for traps baited with e7,9-10:coonbu, percent disruptions were 94.4% and 92.1% for septa (1 g pheromone/ha, 1-wk tri ...201526078301
parasitism, emergence, and development of spalangia endius (hymenoptera: pteromalidae) in pupae of different ages of bactrocera cucurbitae (diptera: tephritidae).the wasp spalangia endius walker (hymenoptera: pteromalidae) is a major parasitoid of the pupae of fruit flies, which are a common agricultural pest. an understanding of this intricate host-parasitoid interaction could provide basic information necessary for the sustainable integrated biological control of fruit flies. in this study, we investigated the effect of s. endius on different-aged pupae of the melon fly bactrocera cucurbitae coquillett by using choice and nonchoice tests under laborato ...201525700538
implementing a spinosad-based local bait station to control bactrocera cucurbitae (diptera: tephritidae) in high rainfall areas of reunion island.three species of fruit flies cause serious damage to cucurbit crops on reunion island: bactrocera cucurbitae (diptera: tephritidae) (coquillett 1899), dacus ciliatus (loew 1901), and dacus demmerezi (bezzi 1917). to control them, a program of agroecological management of cucurbit flies has been implemented based on the application of synéis-appât, especially spot sprays on corn borders. however, the high rainfall on reunion island limits the long-term efficiency of the bait; in addition, this me ...201525688089
assessment of sauromatum guttatum lectin toxicity against bactrocera cucurbitae.lectins are proteins that bind specifically to foreign glycans. due to this binding property, these molecules have potential application as bioinsecticidal tools replacing conventional chemical insecticides. the present study involved purification of phytolectin from the tubers of sauromatum guttatum by affinity chromatography on asialofetuin-linked silica matrix. the purity of the sample was checked by sds-page at ph 8.3. purified lectin was incorporated in the artificial diet of a dipteran mod ...201526688959
a review of the current knowledge on zeugodacus cucurbitae (coquillett) (diptera, tephritidae) in africa, with a list of species included in zeugodacus.this paper reviews all available information regarding the occurrence and biology of the melon fly, zeugodacus cucurbitae (coquillett), in the afrotropical region, including data on invasion history, distribution patterns, population genetics, host range, and interspecific competition. although limited intraspecific variability has been observed within the region regarding the above mentioned aspects, there seems to be no indication that zeugodacus cucurbitae represents a species complex. a chec ...201526798277
melon fly, bactrocera cucurbitae (diptera: tephritidae), infestation in host fruits in the southwestern islands of japan before the initiation of island-wide population suppression, as recorded in publications of japanese public institutions.bactrocera cucurbitae (coquillett) is a tephritid fruit fly native to the indo-malayan region. its distribution, though, has extended to include africa, temperate asia, and a number of pacific islands. it became established in japan in 1919 in the yaeyama islands and spread north in the southwestern islands of japan. it was subsequently eradicated from these islands by an eradication program that extended from 1972 to 1993. as part of an effort to develop a worldwide database on the status of fr ...201526816487
resolving cryptic species complexes of major tephritid pests.an fao/iaea co-ordinated research project (crp) on "resolution of cryptic species complexes of tephritid pests to overcome constraints to sit application and international trade" was conducted from 2010 to 2015. as captured in the crp title, the objective was to undertake targeted research into the systematics and diagnostics of taxonomically challenging fruit fly groups of economic importance. the scientific output was the accurate alignment of biological species with taxonomic names; which led ...201526798252
a novel polysaccharide in insects activates the innate immune system in mouse macrophage raw264 cells.a novel water-soluble polysaccharide was identified in the pupae of the melon fly (bactrocera cucurbitae) as a molecule that activates the mammalian innate immune response. we attempted to purify this innate immune activator using nitric oxide (no) production in mouse raw264 macrophages as an indicator of immunostimulatory activity. a novel acidic polysaccharide was identified, which we named "dipterose", with a molecular weight of 1.01 × 10(6) and comprising nine monosaccharides. dipterose was ...201425490773
methoprene application and diet protein supplementation to male melon fly, bactrocera cucurbitae, modifies female remating behavior.methoprene (an analogue of juvenile hormone) application and feeding on a protein diet is known to enhance male melon fly, bactrocera cucurbitae coquillett (diptera: tephritidae), mating success. in this study, we investigated the effect of these treatments on male b. cucurbitae's ability to inhibit female remating. while 14-d-old females were fed on protein diet, 6-d-old males were exposed to one of the following treatments: (i) topical application of methoprene and fed on a protein diet; (ii) ...201424376160
effect of metarhizium anisopliae on the fertility and fecundity of two species of fruit flies and horizontal transmission of mycotic infection.in mauritius, the peach fruit fly, bactrocera zonata saunders (diptera: tephritidae), and the melon fly, bactrocera cucurbitae (coquillett), are the major pest of fruits and vegetables, respectively. fruit growers make use of broad-spectrum insecticides to protect their crops from fruit fly attack. this method of fruit fly control is hazardous to the environment and is a threat to beneficial insects. the entomopathogenic fungus, metarhizium anisopliae (metchnikoff) sorokin (hypocreales: clavicip ...201425201230
the complete nucleotide sequence of the mitochondrial genome of bactrocera minax (diptera: tephritidae).the complete 16,043 bp mitochondrial genome (mitogenome) of bactrocera minax (diptera: tephritidae) has been sequenced. the genome encodes 37 genes usually found in insect mitogenomes. the mitogenome information for b. minax was compared to the homologous sequences of bactrocera oleae, bactrocera tryoni, bactrocera philippinensis, bactrocera carambolae, bactrocera papayae, bactrocera dorsalis, bactrocera correcta, bactrocera cucurbitae and ceratitis capitata. the analysis indicated the structure ...201424964138
competitive interactions between immature stages of bactrocera cucurbitae (coquillett) and bactrocera tau (walker) (diptera: tephritidae) under laboratory conditions.the melon fly, bactrocera cucurbitae (coquillett), and the pumpkin fly, bactrocera tau (walker), are economically important pests that attack mainly cucurbitacean fruits. the two fruit fly species have similar natural distributions, host ranges, and population growth capacities. this study was designed to assess the asymmetrical competitions through resource exploitation between the larvae of b. cucurbitae and b. tau at different density levels and temperatures, and on different hosts by compari ...201427193811
antimicrobial, phytochemical and larvicidal properties of jatropha multifida linn.to evaluate the phytochemical, antimicrobial and insecticidal properties of different extracts of jatropha multifida (j. multifida) in mauritius.201425312154
a comparison of polyphenolic content, antioxidant activity and insecticidal properties of jatropha species and wild ricinus communis l. found in mauritius.to evaluate the phytochemical, antimicrobial and insecticidal properties of different jatropha species and ricinus communis (castor) (r. communis) growing in mauritius.201425312155
a rapid diagnostic technique of bactrocera cucurbitae and bactrocera zonata (diptera: tephritidae) for quarantine application. 201323152149
genetic correlation between the pre-adult developmental period and locomotor activity rhythm in drosophila melanogaster.biological clocks regulate various behavioural and physiological traits; slower circadian clocks are expected to slow down the development, suggesting a potential genetic correlation between the developmental period and circadian rhythm. however, a correlation between natural genetic variations in the developmental period and circadian rhythm has only been found in bactrocera cucurbitae. the number of genetic factors that contribute to this genetic correlation is largely unclear. in this study, ...201323211793
cold storage enhances the efficacy and margin of security in postharvest irradiation treatments against fruit flies (diptera: tephritidae).cold storage is used to preserve fruit quality after harvest during transportation in marketing channels. low temperature can be a stressor for insects that reduces survivorship, and cold storage may contribute to the efficacy of postharvest quarantine treatments such as irradiation against quarantine insect pests. the combined effect of irradiation and cold storage was examined in a radiation-tolerant fruit fly, bactrocera cucurbitae coquillet (melon fly), and a radiation-intolerant fruit fly, ...201324224244
purification of colocasia esculenta lectin and determination of its anti-insect potential towards bactrocera cucurbitae.the present study reports the purification of a lectin from colocasia esculenta (l.) schott corms and evaluation of its anti-insect potential towards bactrocera cucurbitae (coquilett). the lectin was found to be specific towards n-acetyl-d-lactosamine (lacnac), a disaccharide and asialofetuin, a desialylated serum glycoprotein in hemagglutination inhibition assay. asialofetuin was used as a ligand to purify colocasia esculenta agglutinin (cea) by affinity chromatography. the purity of cea was as ...201324006804
effects of diet and host access on fecundity and lifespan in two fruit fly species with different life history patterns.the reproductive ability of female tephritids can be limited and prevented by denying access to host plants and restricting the dietary precursors of vitellogenesis. the mechanisms underlying the delayed egg production in each case are initiated by different physiological processes that are anticipated to have dissimilar effects on lifespan and reproductive ability later in life. the egg laying abilities of laboratory reared females of the mediterranean fruit fly (ceratitis capitata wiedmann) an ...201323483775
influence of modified atmosphere packaging on radiation tolerance in the phytosanitary pest melon fly (diptera: tephritidae).modified atmosphere packaging (map) produces a low-oxygen (o2) environment that can increase produce shelf life by decreasing product respiration and growth of pathogens. however, low o2 is known to increase insect tolerance to irradiation, and the use of map with products treated by irradiation before export to control quarantine pests may inadvertently compromise treatment efficacy. melon fly, bactrocera cucurbitae coquillet (diptera: tephritidae), is an important economic and quarantine pest ...201324224242
population dynamics, distribution, and species diversity of fruit flies on cucurbits in kashmir valley, india.given the economic importance of cucurbits and the losses incurred by fruit fly infestation, the population dynamics of fruit flies in cucurbit crops and the influence of abiotic parameters, such as temperature, relative humidity, rainfall, and total sunshine hours per day on the fruit fly population were studied. the study was carried out at six locations; in district srinagar the locations were batmaloo, shalimar, and dal, while in district budgam the locations were chadoora, narkara, and buga ...201323906383
genetic and cytogenetic characterization of genetic sexing strains of bactrocera dorsalis and bactrocera cucurbitae (diptera: tephritidae).in the current study, we performed genetic and cytogenetic analyses of two genetic sexing strains (gsss), one for bactrocera dorsalis s.s. and one for melon fly, bactrocera cucurbitae coquillett, the first such strains ever constructed in these species. in both strains, the genetic sexing mechanism is based on a pupal color dimorphism (white or brown) and is the result of a reciprocal translocation between the y chromosome and the autosome bearing the white pupae (wp) locus. based on genetic ana ...201323786092
effect of oxytetracycline and sulphanilamide on the reproductive organs and fecundity of melon fly, dacus cucurbitae coq. 20131233354
the complete mitochondrial genome of the melon fly bactrocera cucurbitae (diptera: tephritidae).the mitochondrial genome of bactrocera cucurbitae, a representative of the tephritid family, was completely sequenced for the first time. the b. cucurbitae genome is a double-stranded circular molecule of 15,825 bp long, including the entire set of the 37 genes. the 72.9% a+t content and 0.047 at-skew are within the range of the known dipteran genomes. comparative analyses showed that dipteran mitochondrial protein-coding genes present complex evolutionary patterns. some of the codon families we ...201322931686
monitoring resistance to spinosad in the melon fly (bactrocera cucurbitae) in hawaii and taiwan.spinosad is a natural insecticide with desirable qualities, and it is widely used as an alternative to organophosphates for control of pests such as the melon fly, bactrocera cucurbitae (coquillett). to monitor the potential for development of resistance, information about the current levels of tolerance to spinosad in melon fly populations were established in this study. spinosad tolerance bioassays were conducted using both topical applications and feeding methods on flies from field populatio ...201222629193
species diversity within a community of the curcurbit fruit flies bactrocera cucurbitae, dacus ciliatus, and dacus demmerezi roosting in corn borders near cucurbit production areas of reunion island.in order to better control fruit flies (diptera: tephritidae) attacking cucurbitaceae on reunion island (21°6 s/ 55°36 e), biological characteristics (seasonal fluctuation, relative abundance, sex ratio) of communities roosting in corn borders were investigated. the study was conducted in austral summer across a range of altitudes (750-1150 m) corresponding to the main areas of cucurbit cropping. living adults were recorded roosting on corn planted within or around cucurbit fields. results showe ...201222958393
cage study of spinosad-based bait efficacy on bactrocera cucurbitae, dacus ciliatus, and dacus demmerezi (diptera: tephritidae) in reunion island.on reunion island, bactrocera cucurbitae (coquillett), dacus ciliatus (loew), and dacus demmerezi (bezzi) cause severe damage to cucurbit crops. the aim of the study was to test in field cages the effectiveness of synéis-appât (dow agrosciences), a spinosad-based bait (0.02% of spinosad) on both attraction and mortality of young adults (6-9 d old) of these three species. the effects of gender were also evaluated for all species whereas the effects of protein deprivation were tested with b. cucur ...201222928317
population genetic structure of the melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae) based on mitochondrial cytochrome oxidase (coi) gene sequences.population genetic structure of melon fly analysed with mitochondrial cytochrome oxidase i gene suggested that melon fly populations across the globe is homogeneous with non-significant variation of 0.000-0.003 base substitutions per site. test isolates representing various geographic situations across the world were placed in 26 mitochondrial haplotypes based on variations associated with a maximum of three mutational steps and the predominant haplotype i.e. h1 was present in all melon fly popu ...201222660946
regional suppression of bactrocera fruit flies (diptera: tephritidae) in the pacific through biological control and prospects for future introductions into other areas of the world.bactrocera fruit fly species are economically important throughout the pacific. the usda, ars u.s. pacific basin agricultural research center has been a world leader in promoting biological control of bactrocera spp. that includes classical, augmentative, conservation and ipm approaches. in hawaii, establishment of bactrocera cucurbitae (coquillett) in 1895 resulted in the introduction of the most successful parasitoid, psyttalia fletcheri (silvestri); similarly, establishment of bactrocera dors ...201226466626
resveratrol modifies tephritid fruit fly response to radiation but not nutritional stress.resveratrol (3,5,4'-trihydroxystilbene) is a polyphenol compound found in many plants and fruits that has antioxidant and radioprotective properties. two model invertebrates, bactrocera dorsalis (oriental fruit fly) and b. cucurbitae (melon fly) (diptera: tephritidae), were studied to determine if the addition of resveratrol to an artificial diet could modify their response to radiation and nutritional stress.201222145646
field trials of solid triple lure (trimedlure, methyl eugenol, raspberry ketone, and ddvp) dispensers for detection and male annihilation of ceratitis capitata, bactrocera dorsalis, and bactrocera cucurbitae (diptera: tephritidae) in hawaii.solid mallet tmr (trimedlure [tml], methyl eugenol [me], raspberry ketone [rk]) wafers and mallet cmr (ceralure, me, rk, benzyl acetate) wafers impregnated with ddvp (2,2-dichlorovinyl dimethyl phosphate) insecticide were measured in traps as potential detection and male annihilation technique (mat) devices. comparisons were made with 1) liquid lure and insecticide formulations, 2) solid cones and plugs with an insecticidal strip, and 3) solid single and double lure wafers with ddvp for captures ...201223156150
trap capture of three economically important fruit fly species (diptera: tephritidae): evaluation of a solid formulation containing multiple male lures in a hawaiian coffee field.invasive fruit flies (diptera: tephritidae) pose a global threat to agriculture through direct damage to food crops and the accompanying trade restrictions that often result. early detection is vital to controlling fruit flies, because it increases the probability of limiting the growth and spread of the invasive population and thus may greatly reduce the monetary costs required for eradication or suppression. male-specific lures are an important component of fruit fly detection, and three such ...201222928297
generic phytosanitary radiation treatment for tephritid fruit flies provides quarantine security for bactrocera latifrons (diptera: tephritidae).bactrocera latifrons (hendel) (diptera: tephritidae) is a quarantine pest of several solanaceous crops and tropical fruits that are treated using irradiation before export from hawaii to the u.s. mainland. a dose of 150 gy is approved as a generic irradiation treatment for tephritid fruit flies, but no confirmation of efficacy has been reported for b. latifrons. dose response of b. latifrons was used to determine the most tolerant life stage and identify a dose that prevents adult emergence. dat ...201122066179
a compound produced by fruigivorous tephritidae (diptera) larvae promotes oviposition behavior by the biological control agent diachasmimorpha longicaudata (hymenoptera: braconidae).tephritid fruit fly parasitoids use fruit-derived chemical cues and the vibrations that result from larval movements to locate hosts sequestered inside fruit. however, compounds produced by the larvae themselves have not been previously described nor their significance to parasitoid foraging determined. we collected the volatiles from four species of tropical and subtropical tephritidae: anastrepha suspensa (loew), bactrocera dorsalis hendel, bactrocera cucurbitae coquillett, and ceratitis capit ...201122251652
assessment of attractiveness of cassava as a roosting plant for the melon fly, bactrocera cucurbitae, and the oriental fruit fly, b. dorsalis.application of bait spray to crop borders is a standard approach for suppression of melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae) populations and may also be of value for suppression of oriental fruit fly, b. dorsalis (hendel) populations. establishment of preferred roosting hosts as crop borders may help to improve suppression of both fruit fly species by providing sites for bait spray applications. in an area-wide b. cucurbitae suppression trial, the question was raised ...201121526935
response of ceratitis capitata, bactrocera dorsalis, and bactrocera cucurbitae (diptera: tephritidae) to metabolic stress disinfection and disinfestation treatment.metabolic stress disinfection and disinfestation (msdd) is a postharvest treatment designed to control pathogens and arthropod pests on commodities that combines short cycles of low pressure/vacuum and high co2 with ethanol vapor. experiments were conducted to evaluate the effect of msdd treatment on various life stages of ceratitis capitata (wiedemann), mediterranean fruit fly; bactrocera dorsalis hendel, oriental fruit fly; and bactrocera cucurbitae coquillett, melon fly, in petri dishes and i ...201121404842
a comparative assessment of the response of three fruit fly species (diptera: tephritidae) to a spinosad-based bait: effect of ammonium acetate, female age, and protein hunger.ammonia-releasing substances are known to play an important role in fruit fly (diptera: tephritidae) attraction to food sources, and this information has been exploited for the development of effective synthetic food-based lures and insecticidal baits. in field studies conducted in hawaii, we examined the behavioural response of wild female oriental fruit fly (bactrocera dorsalis (hendel)), melon fly (b. cucurbitae (coquillett)), and mediterranean fruit fly (ceratitis capitata (wiedemann)) to sp ...201120961468
microsatellite markers reveal population structure and low gene flow among collections of bactrocera cucurbitae (diptera: tephritidae) in asia.the melon fruit fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), is widespread agricultural pest, and it is known to have the potential to establish invasive populations in various tropical and subtropical areas. despite the economic risk associated with a putative stable presence of this fly, the population genetics of this pest have remained relatively unexplored in asia, the main area for distribution of this pest. the goals for this study were to employ nuclear markers to exam ...201121735930
host status of vaccinium reticulatum (ericaceae) to invasive tephritid fruit flies in hawaii.ohelo (vaccicinium reticulatum small) (ericaceae) is a native hawaiian plant that has commercial potential in hawaii as a nursery crop to be transplanted for berry production or for sale as a potted ornamental. no-choice infestation studies were conducted to determine whether ohelo fruit are hosts for four invasive tephritid fruit fly species. ohelo berries were exposed to gravid female flies ofbactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit f ...201121510206
susceptibility of low-chill blueberry cultivars to mediterranean fruit fly, oriental fruit fly, and melon fly (diptera: tephritidae).no-choice tests were conducted to determine whether fruit of southern highbush blueberry, vaccinium corymbosum l., hybrids are hosts for three invasive tephritid fruit flies in hawaii. fruit of various blueberry cultivars was exposed to gravid female flies of bactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit fly), or bactrocera cucurbitae coquillet (melon fly) in screen cages outdoors for 6 h and then held on sand in the laboratory for 2 wk for ...201121510205
the clock gene cryptochrome of bactrocera cucurbitae (diptera: tephritidae) in strains with different mating times.differences in mating time between populations can give rise to premating reproductive isolation. tephritid fruit flies exhibit large variation in mating time among intra- or inter-specific populations. we previously cloned the clock gene period from two strains of melon fly, bactrocera cucurbitae; in one the individuals mate early during the day, whereas in the other the individuals mate later. these strains were originally established by divergent artificial selection for developmental time, ' ...201020010960
capture probability of released males of two bactrocera species (diptera: tephritidae) in detection traps in california.the genus bactrocera (diptera: tephritidae) includes approximately 70 polyphagous species that are major pests of fruit and vegetable crops. most bactrocera species have limited geographic distributions, but several species are invasive, and many countries operate continuous trapping programs to detect infestations. in the united states, california maintains approximately 25,000 traps (baited with male lures) specifically for bactrocera detection distributed over an area of approximately 6,400 k ...201021309224
response of melon fly (diptera: tephritidae) to weathered splat-spinosad-cue-lure.studies were conducted in hawaii to measure attraction of male melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), to splat-cue-lure (c-l) and splat-melo-lure (m-l) (raspberry ketone formate). direct field comparisons of splat-c-l and splat-m-l at low (5%) and high (20%) concentrations indicated few differences in attraction over a 15-wk period. subsequently, only splat-spinosad-c-l (5%) was compared with min-u-gel c-l with naled (standard used in california) in weathering stud ...201021061958
effects of the juvenile hormone analogue methoprene and dietary protein on male melon fly bactrocera cucurbitae (diptera: tephritidae) mating success.the effect of access to dietary protein (p) and the topical application of a juvenile hormone analogue (methoprene (m)) on mating behaviour of male melon fly bactrocera cucurbitae was assessed in the laboratory and in field cages. age, dietary protein and methoprene application increased the mating success and influenced the mating behaviour. treatment with methoprene (m+) to protein-deprived (p-) males had only a modest effect on the acceleration of sexual maturity, but application of methopren ...201020438735
macrogeographic population structuring in the cosmopolitan agricultural pest bactrocera cucurbitae (diptera: tephritidae).the macrogeographic population structure of the agricultural pest bactrocera cucurbitae (diptera: tephritidae) was investigated in order to identify the geographic origin of the species and reconstruct its range expansion. individuals of b. cucurbitae were collected from 25 worldwide-distributed localities (n = 570) and genotyped at 13 microsatellite loci. the bayesian clustering reveals that b. cucurbitae can be subdivided into five main groups corresponding to populations from (i) the african ...201020561200
recent advances in methyl eugenol and cue-lure technologies for fruit fly detection, monitoring, and control in hawaii.worldwide, an important aspect of invasive insect pest management is more effective, safer detection and control systems. phenyl propanoids are attractive to numerous species of dacinae fruit flies. methyl eugenol (me) (4-allyl-1, 2-dimethoxybenzene-carboxylate), cue-lure (c-l) (4-(p-acetoxyphenyl)-2-butanone), and raspberry ketone (rk) (4-(p-hydroxyphenyl)-2-butanone) are powerful male-specific lures. most evidence suggests a role of me and c-l/rk in pheromone synthesis and mate attraction. me ...201020831962
evaluation of methyl eugenol and cue-lure traps with solid lure and insecticide dispensers for fruit fly monitoring and male annihilation in the hawaii areawide pest management program.methyl eugenol (me) and cue-lure (c-l) traps with solid lure dispensers were deployed in areas with low and high populations of oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett), respectively. in low-density areas, standard jackson traps or hawaii fruit fly areawide pest management (awpm) traps with ft mallet me wafers impregnated with dimethyl dichloro-vinyl phosphate (ddvp) or awpm traps with scentry me cones and vapor tape performed equally as ...201020429456
cucumber volatile blend attractive to female melon fly, bactrocera cucurbitae (coquillett).the melon fly, bactrocera cucurbitae (coquillett), is a serious crop pest throughout the asia pacific sub-continent and southeast asia, causing damage to tree fruits, cucurbits, and related crops. attractants for female melon flies are of particular interest as they could be used in control tactics to reduce pest levels. previous work has shown that freshly sliced cucumbers are attractive to female melon flies, but the compounds responsible for this attraction were not identified. the objective ...201020526732
permanent genetic resources added to molecular ecology resources database 1 december 2009-31 january 2010.this article documents the addition of 220 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: allanblackia floribunda, amblyraja radiata, bactrocera cucurbitae, brachycaudus helichrysi, calopogonium mucunoides, dissodactylus primitivus, elodea canadensis, ephydatia fluviatilis, galapaganus howdenae howdenae, hoplostethus atlanticus, ischnura elegans, larimichthys polyactis, opheodrys vernalis, pelteobagrus fulvidraco, phragmidiu ...201021565062
total body nitrogen and total body carbon as indicators of body protein and body lipids in the melon fly bactrocera cucurbitae: effects of methoprene, a juvenile hormone analogue, and of diet supplementation with hydrolyzed yeast.the application of methoprene, and providing access to diet including hydrolyzed yeast, are treatments known to enhance mating success in the male melon fly bactrocera cucurbitae coquillett (diptera: tephritidae), supporting their use in mass rearing protocols for sterile males in the context of sterile insect technique (sit) programmes. the objective of the present laboratory study was to investigate the effect of methoprene application and diet supplementation with hydrolyzed yeast (protein) o ...201020688076
captures of pest fruit flies (diptera: tephritidae) and nontarget insects in biolure and torula yeast traps in hawaii.multilure traps were deployed in a hawaiian orchard to compare the attraction of economically important fruit flies and nontarget insects to the three-component biolure and torula yeast food lures. either water or a 20% propylene glycol solution was used to dissolve the torula yeast or as capture fluid in biolure traps. torula yeast in water was more attractive than biolure for male and female bactrocera cucurbitae (coquillett) and bactrocera dorsalis (hendel) and as attractive for ceratitis cap ...201022546461
development of allele-specific single-nucleotide polymorphism-based polymerase chain reaction markers in cytochrome oxidase i for the differentiation of bactrocera papayae and bactrocera carambolae (diptera: tephritidae).differentiation of bactrocera papayae drew & hancock and bactrocera carambolae drew & hancock (diptera: tephritidae) based on morphological characters has often been problematical. we describe here a single-nucleotide polymorphism (snp)-based polymerase chain reaction (pcr) assay to differentiate between these two species. for detection of snps, fragments derived from each species were amplified using two primer pairs, coif/coir and uea7/uea10, sequenced, and aligned to obtain a contiguous 1,517 ...201021309218
host use of bactrocera latifrons, a new invasive tephritid species in tanzania.bactrocera latifrons (hendel) is a pest of asian origin, first detected in africa in 2006. we assessed the host utilization of this quarantine pest in morogoro region, eastern central tanzania, by collecting a wide range of cultivated and wild host plants of species belonging to solanaceae and cucurbitaceae from april 2007 to april 2008. fruit were collected from 29 plant species and varieties (16 solanaceae and 13 cucurbitaceae) in all agroecological zones of morogoro region. twelve solanaceous ...201020214370
captures in methyl eugenol and cue-lure detection traps with and without insecticides and with a farma tech solid lure and insecticide dispenser.methyl eugenol (me) and cue-lure (c-l) traps to detect tephritid flies on the u.s. mainland were tested with and without insecticides under hawaiian weather conditions against small populations of oriental fruit fly, bactrocera dorsalis (hendel) and melon fly, bactrocera cucurbitae (coquillett), respectively. in comparative tests, standard jackson traps with naled and the hawaii fruit fly areawide pest management (awpm) trap with 2,2-dichorovinyl dimethyl phosphate (ddvp) insecticidal strips out ...200919449634
evaluation of yeasts and yeast products in larval and adult diets for the oriental fruit fly, bactrocera dorsalis, and adult diets for the medfly, ceratitis capitata, and the melon fly, bactrocera curcurbitae.several yeasts and yeast products were tested in adult diets for the medfly ceratitis capitata (wiedemann), oriental fruit fly bactrocera dorsalis (hendel), and melon fly, bactrocera curcurbitae (coquillett) (diptera: tephritidae) and in larval liquid diet for mass-rearing b. dorsalis. three hydrolyzed brewer's yeasts (fnils65, fni200 and fni210), one glutamine enriched yeast (gsh), one vitamin-enriched yeast (rda500), korea yeast, whole cell yeasts, and combinations of them were evaluated. adul ...200919613830
host range and distribution of fruit-infesting pestiferous fruit flies (diptera, tephritidae) in selected areas of central tanzania.the host range of major fruit fly pests in central tanzania was evaluated from october 2004 to october 2006. samples of 48 potential hosts were collected and incubated for fruit fly emergence. bactrocera invadens was the dominant species in incidence expressed as the ratio of infested to total number samples collected, as well as infestation rate, expressed as number of flies emerging per unit weight. eight new host fruits are reported. infestation by native pests, such as ceratitis capitata and ...200919323850
anti-insect potential of lectins from arisaema species towards bactrocera cucurbitae.bactrocera cucurbitae (coquillett), also known as melon fruit fly, is one of the major insect pests of cucurbits in several parts of asia, africa and pacific. in the present investigation, effect of lectins from two sources i.e. arisaema intermedium blume and arisaema wallichianum hook f. (family-araceae) has been studied on the development of second instar larvae of melon fruit fly. the lectins were incorporated separately in artificial diet at a concentration of 10 to 160 microg ml(-1) and fed ...200920329399
effects of indian coral tree, erythrina indica lectin on eggs and larval development of melon fruit fly, bactrocera cucurbitae.present study was undertaken to investigate the influence of d-galactose binding lectin from erythrina indica lam. on the eggs and second instar larvae (64-72 hr) of melon fruit fly, bactrocera cucurbitae (coquillett). the lectin from e. indica seeds was extracted and purified by affinity chromatography using asilofetuin linked porous amino activated silica beads. the effects of various concentrations (0, 125, 250, 500 and 1000 microg ml(-1)) of lectin were studied on freshly laid eggs (0-8 hr) ...200920120488
host status of blueberry to invasive tephritid fruit flies in hawaii.forced infestation studies were conducted to determine whether northern or southern highbush blueberries, vaccinium corymbosum l., are hosts for the invasive tephritid fruit flies in hawaii. fruit were exposed to gravid female flies of bactrocera dorsalis hendel (oriental fruit fly), ceratitis capitata (wiedemann) (mediterranean fruit fly), or bactrocera cucurbitae coquillet (melon fly) in screen cages outdoors for 6 h and then held on sand in the laboratory for 2 wk for pupal and adult emergenc ...200919886450
polymorphic microsatellite markers in the melon fruit fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae).bactrocera cucurbitae (coquillett) is an important insect pest of melon fruit causing extensive losses of production in asia and pacific areas. twelve polymorphic microsatellite loci were isolated from an enriched dna library of this species and their amplification characteristics are described. genetic parameters were estimated on 80 individual flies from four natural populations. allele numbers per locus ranged from three to 20. these microsatellite markers have potential utility in population ...200921564920
thermal death kinetics of mediterranean, malaysian, melon, and oriental fruit fly (diptera: tephritidae) eggs and third instars.the late-aged egg and third-instar life stages of laboratory-reared malaysian fruit fly, bactrocera latifrons (hendel); mediterranean fruit fly, ceratitis capitata (wiedemann); melon fly, b. cucurbitae coquillett; and oriental fruit fly, b. dorsalis (hendel), (diptera: tephritidae); and the third instars of wild mediterranean fruit fly were exposed to thermal treatments. a heating block system was used to determine the thermal death kinetics of the four fruit fly species. treatments consisted of ...200919449631
insecticidal activity of basil oil, trans-anethole, estragole, and linalool to adult fruit flies of ceratitis capitata, bactrocera dorsalis, and bactrocera cucurbitae.basil oil and its three major active constituents (trans-anethole, estragole, and linalool) obtained from basil (oscimum basilicum l.) were tested on three tephritid fruit fly species [ceratitis capitata (wiedemann), bactrocera dorsalis (hendel), and bactrocera cucurbitae (coquillett)] for insecticidal activity. all test chemicals acted fast and showed a steep dose-response relationship. the lethal times for 90% mortality/knockdown (lt90) of the three fly species to 10% of the test chemicals wer ...200919253638
an evaluation of alternative insecticides to diazinon for control of tephritid fruit flies (diptera: tephritidae) in soil.diazinon has been used extensively in the past as part of california eradication programs for tephritid fruit flies (diptera: tephritidae) as a soil drench, but it is being phased out for this purpose in the united states. therefore, in this study, the toxicity of platinum, force, admire, regent, and warrior was estimated after application to sand and soil as drenches for control of the mediterranean fruit fly, ceratitis capitata (wiedemann); melon fly, bactrocera cucurbitae (coquillett); and or ...200919253629
effects of plant lectin from cobra lily, arisaema curvatum kunth on development of melon fruit fly, bactrocera cucurbitae (coq.).the lectin from tubers of cobra lily, arisaema curvatum kunth was purified by affinity chromatography using asialofetuin-linked amino activated porous silica beads. the concentration dependent effect of lectin was studied on second instar larvae (64-72 hr) of bactrocera cucurbitae (coq.). the treatment not only resulted in a significant reduction in the percentage pupation and emergence of the adults from treated larvae but it also prolonged the remaining larval development period. a very low lc ...200819297991
development of immature stages and comparative demography of two cucurbit-attacking fruit flies in reunion island: bactrocera cucurbitae and dacus ciliatus (diptera tephritidae).on reunion island, two species of dacini, bactrocera cucurbitae (coquillett) and dacus ciliatus loew, infest 16 host plant species belonging to the family cucurbitaceae from sea level to 1,600 m. these two species represent two primary pests of this plant family on the island. melon fly, bactrocera cucurbitae, and ethiopian fruit fly, d. ciliatus, larval development was studied at four different constant temperatures (15, 20, 25, and 30 degrees c) with three host plants (cucumber, pumpkin, and s ...200818419901
evaluation of lure dispensers for fruit fly surveillance in new zealand.fruit flies (diptera: tephritidae) represent a major biosecurity threat to the horticulture sector of new zealand, which is entirely free of these invasive pests. a nationwide surveillance programme is conducted to ensure any incursion is detected as early as possible. a review of the lure dispensers used is reported here.200818383565
evaluation of splat with spinosad and methyl eugenol or cue-lure for "attract-and-kill" of oriental and melon fruit flies (diptera: tephritidae) in hawaii.specialized pheromone and lure application technology (splat) methyl eugenol (me) and cue-lure (c-l) "attract-and-kill" sprayable formulations containing spinosad were compared with other formulations under hawaiian weather conditions against oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), respectively. field tests were conducted with three different dispensers (min-u-gel, acti-gel, and splat) and two different insectici ...200818613576
population genetic structure of the melon fly, bactrocera cucurbitae (diptera: tephritidae), from china and southeast asia.the melon fly, bactrocera cucurbitae coquillett, is a species of fruit flies of significant agricultural interest. of supposed indian origin, the melon fly is now widely distributed throughout south east asia up to china, while it has been recently eradicated from japan. the population structure of seven geographic populations from coastal china, as well as samples from other regions of south east asia and japan, including lab colonies, have been studied using a 782 bp fragment of mitochondrial ...200818188668
attraction of ethyl ether extracts of 232 botanicals to oriental fruit flies, melon flies, and mediterranean fruit flies.the mediterranean fruit fly, ceratitis capitata (wiedemann), the melon fly, dacus cucurbitae coquillett, and the oriental fruit fly, d. dorsalis hendel, three hawaiian tephritids of economic importance, were exposed to traps each containing one of 232 ethyl ether extracts of air-dried botanicals. the principal concern was to establish which extracts were attractive judged on the basis of an index determined by the number of times more flies were attracted to the trap baited with water and extrac ...20081134212
impact of natural hosts and artificial adult diets on some quality parameters of the melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae).experiments were conducted to evaluate the impact of different natural hosts and artificial adult diets on the pupal quality, adult emergence, ovariole number and longevity of the melon fly, bactrocera cucurbitae (coquillett) for two generations under laboratory condition. pupal quality and percentage adult emergence was slightly higher in f2 generation than f1 generation from all tested hosts. b. cucurbitae fed on proteose-peptone sugar (1:4) produced twice as many as eggs when fed on yeast:sug ...200719070011
effects of neem leaf dust and a commercial formulation of a neem compound on the longevity, fecundity and ovarian development of the melon fly, bactrocera cucurbitae (coquillett) and the oriental fruit fly, bactrocera dorsalis (hendel) (diptera: tephritidae).neem leaf dust and a commercial formulation of neem were tested on adult bactrocera cucurbitae (coquillett) and bactrocera dorsalis (hendel) to determine their effects on the longevity, fecundity and ovarian development. different combinations of neem leaf dust and a commercial formulation of a neem compound incorporated with sugar solution and adult rearing diets were tested. the laboratory tests showed that ingestion of neem can significantly reduced the longevity and fertility of both the fly ...200719093477
assessment of attractiveness of plants as roosting sites for the melon fly, bactrocera cucurbitae, and oriental fruit fly, bactrocera dorsalis.the use of toxic protein bait sprays to suppress melon fly, bactrocera cucurbitae (coquillett) (diptera: tephritidae), populations typically involves application to vegetation bordering agricultural host areas where the adults seek shelter ("roost"). although bait spray applications for suppression of oriental fruit fly, bactrocera dorsalis (hendel), populations have traditionally been applied to the host crop, rather than to crop borders, roosting by oriental fruit flies in borders of some crop ...200720334596
field captures of wild melon fly (diptera: tephritidae) with an improved male attractant, raspberry ketone formate.field-trapping evaluations of the new male attractant, formic acid 4-(3-oxobutyl) phenyl ester (raspberry ketone formate [rkf]) were conducted in hawaii with wild populations of melon flies, bactrocera cucurbitae coquillett (diptera: tephritidae), to determine its activity in the field and to evaluate new plastic matrix formulations. all tests were compared with the standard melon fly attractant 4-(4-acetoxyphenyl) -2-butanone (cuelure [cl]), which is the attractant of choice for detection progr ...200717849860
effectiveness of protein baits on melon fly and oriental fruit fly (diptera: tephritidae): attraction and feeding.attraction and feeding responses of oriental fruit fly, bactrocera dorsalis (hendel), and melon fly, bactrocera cucurbitae (coquillett), were determined for different protein baits. in separate choice attraction assays for each species, significantly more flies arrived at stations with bait than water, but no differences existed among baits of gf-120 fruit fly bait, gf-120 nf naturalyte fruit fly bait, provesta 621 autolyzed yeast extract, and mazoferm e802. in comparison with b. dorsalis, b. cu ...200616937668
a tuber lectin from arisaema jacquemontii blume with anti-insect and anti-proliferative properties.a tuber lectin from arisaema jacquemontii blume belonging to family araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mm glycine-hcl buffer ph 2.5. the purified a. jacquemontii lectin (ajl) showed a single protein band with an apparent molecular mass of 13.4 kda when submitted to sds-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. t ...200616889688
a tuber lectin from arisaema helleborifolium schott with anti-insect activity against melon fruit fly, bactrocera cucurbitae (coquillett) and anti-cancer effect on human cancer cell lines.an anti-insect and anti-cancer lectin has been isolated from arisaema helleborifolium schott by affinity chromatography using asialofetuin-linked amino activated silica beads. the bound a. helleborifolium lectin (ahl) was eluted with 100mm glycine-hcl buffer, ph 2.5. it gave a single band on sds-page, ph 8.3, and page, ph 4.5. however, multiple bands were obtained in page at ph 8.3 and isoelectric focusing. the lectin was a homotetramer having subunit molecular mass 13.4kda while its native mole ...200616321359
new contributions towards the understanding of the phylogenetic relationships among economically important fruit flies (diptera: tephritidae).fruit flies (diptera: tephritidae) are a species-rich and economically important group. the phylogenetic relationships among the many taxa are still to be fully resolved and the monophyly of several groups is still to be confirmed. this paper reports a study of the phylogenetic relationships among 23 economically important tephritid species (representing several major lineages of the family) which examines the sequence of a region of mitochondrial dna encompassing the cytb, trna(ser) and nd1 gen ...200616768816
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