sphingobacterium multivorum isolated from a patient with cystic fibrosis. 19921563393
occurrence of o-glycosidically peptide-linked oligosaccharides of poly-n-acetyllactosamine type (erythroglycan ii) in the i-antigenically active sendai virus receptor sialoglycoprotein gp-2.unique high molecular weight (m.w. 4,000-9,000) sugar chains termed erythroglycan ii have been obtained from alkali/sodium borohydride digests of i-active asialoglycoprotein derived from sialoglycoprotein gp-2, which was isolated recently from bovine erythrocyte membranes as sendai virus receptor (suzuki, y. et al. (1983) j. biochem. 93, 1621-1633; (1984) ibid, 95, 1193-1200). it was found that these sugar chains comprise about 40% of total alkali-labile oligosaccharides of asialo gp-2 and conta ...19853005249
substrate specificity of endo-beta-galactosidases from flavobacterium keratolyticus and escherichia freundii is different from that of pseudomonas sp.the substrate specificity of endo-beta-galactosidase of pseudomonas sp. was found to differ from that of flavobacterium keratolyticus or escherichia freundii, based on the following experimental results. the endo-beta-galactosidases from these three bacteria released 6-o-sulfo-glcnac beta 1-3gal as one of the major products from keratan sulfates from different sources. in addition to the sulfated disaccharide, flavobacterium and escherichia enzymes produced glcnac beta 1-3gal, which is also an i ...19863096984
structure and immunochemistry of an oligosaccharide repeating unit of the capsular polysaccharide of type iii group b streptococcus. a revised structure for the type iii group b streptococcal polysaccharide antigen.we have derived oligosaccharides from the capsular polysaccharide of type iii group b streptococcus by enzymatic hydrolysis of a specific backbone glycosidic bond utilizing an endo-beta-galactosidase from flavobacterium keratolyticus. enzymatic digestion of the polysaccharide produced oligosaccharide fragments of one or more pentasaccharide repeating units. on the basis of 13c nmr, 1h nmr, and methylation analyses, it was established that the smallest digestion fragment was alpha-d-neupnac-(2--- ...19873298228
septicemia caused by sphingobacterium multivorum.sphingobacterium multivorum was isolated in pure culture from the blood of a man undergoing chemotherapy for a lymphoma. he responded to appropriate antibiotics and later showed a significant rise in specific serum antibodies. carbon substrate assimilation tests and fatty acid analysis proved useful in identifying the organism precisely.19873597758
[isolation and description of strains of flavobacterium multivorum of telluric origin].twenty encapsulated strains of flavobacterium multivorum were isolated from soil by elective culture in minimal medium containing inulin as the sole source of carbon and energy. these strains were compared with the type strain and with 5 other strains (including 4 clinical strains) of f. multivorum. of 168 substrates tested, 18 carbohydrates were used as the sole carbon and energy source by all 26 strains. a few amino acids were used by some strains. the yellow pigment produced was found to be a ...19854051455
lectin-binding activity and antibody response with pseudomonas paucimobilis and flavobacterium multivorum.pseudomonas paucimobilis and flavobacterium multivorum (formerly group iik biotype 1 and biotype 2, respectively) showed lectin-binding activity with helix aspersa and no activity with eight other well-characterized lectins. microagglutination titrations and immunodiffusion precipitation revealed specific antibody activity to immunizing antigens. however, no major antigens were found to be common from crude antigen preparations of p. paucimobilis and f. multivorum when tested against opposing an ...19836411314
[endo-beta-galactosidase from flavobacterium keratolyticus and escherichia freundii]. 19846431498
in vitro activity of cefoperazone against nonfermenters and aeromonas hydrophila.the in vitro activity of cefoperazone against 380 strains (33 species and unnamed groups) of nonferenters and 20 strains of aeromonas hydrophila was studied by a microdilution method of determining minimal inhibitory concentrations. for comparison, the activities of ampicillin, ticarcillin, and cefamandole were simultaneously studied. cefoperazone was the most active drug against pseudomonas sp., achromobacter xylosoxidans, flavobacterium meningosepticum. flavobacterium sp. (group iib), group iv ...19806448581
isolation and identification of ropy bacteria in raw milk.approximately 4.2% of 4,000 maryland-virginia raw milk tanker samples developed ropiness when incubated at 10 degrees c. of the 56 bacterial isolates 30 were identified by species. klebsiella oxytoca and pseudomonas aeruginosa were isolated most frequently. other ropy isolates were identified as pseudomonas spp., chromobacterium, flavobacterium multivorum, presumptive yersinia pestis, enterobacter agglomerans, klebsiella pneumoniae, and pasteurella-actinobacter spp. six of the klebsiella oxytoca ...19836685140
flavobacterium multivorum septicemia in a hemodialyzed patient.a case of flavobacterium multivorum septicemia in a hemodialyzed patient is reported. two blood cultures were positive at 48 h, and the patient became afebrile only after antimicrobial therapy. the origin of the septicemia could not be determined.19846715525
isolation and characterization of a keratan sulfate-degrading endo-beta-galactosidase from flavobacterium keratolyticus. 19816783650
endo-beta-galactosidase from flavobacterium keratolyticus. 19826808310
enzymatic profile of pseudomonas paucimobilis.pseudomonas paucimobilis, previously known as group iik biotype 1, was found to have a significantly different enzyme profile from that of flavobacterium multivorum, formerly group iik biotype 2. p. paucimobilis produces several esterases and phosphatases which may contribute to its virulence.19827130368
endotoxin-like activity in pseudomonas paucimobilis (group iik biotype 1) and flavobacterium multivorum (group iik biotype 2). 19827151972
expression of the symbiotic plasmid from rhizobium leguminosarum biovar trifolii in sphingobacterium inoculant strain of rhizobium leguminosarum biovar trifolii containing a tn5 marked symbiotic plasmid transferred this plasmid by conjugation to sphingobacterium multivorum, an organism that can be found in soil. the transconjugant bacteria nodulated the roots of white clover (trifolium repens) seedlings but did not fix atmospheric nitrogen. microscopic examination revealed abnormal nodule structures. bacteria isolated from the nodules were shown to be closely related to the recipient s. mult ...19948000966
discovery of a new type of sialidase, "kdnase," which specifically hydrolyzes deaminoneuraminyl (3-deoxy-d-glycero-d-galacto-2-nonulosonic acid) but not n-acylneuraminyl linkages.the release of 3-deoxy-d-glycero-d-galacto-2-nonulosonic acid (kdn, deaminoneuraminic acid) residues from their alpha-ketosidic linkage is required to determine the structural and functional role of kdn-glycoconjugates in sources as disparate as trout egg polysialoglycoproteins and human cancers. we report for the first time the isolation and characterization of a novel type of sialidase (kdnase), which specifically hydrolyzes kdn ketosidic but not n-acylneuraminyl linkages. kdnase activity was ...19948063773
[hypersensitivity pneumonitis caused by a home humidifier].a 33-year-old man was admitted complaining of a fever, dyspnea, and a dry cough almost every night since december of 1992. he had been using an ultrasonic humidifier at home. the chest ct scan and roentgenogram showed bilateral reticulonodular shadows. after admission, the symptoms resolved spontaneously. these findings were suggestive of hypersensitivity pneumonitis. after analysis of fluid obtained by bronchoalveolar lavage and of a specimen obtained by transbronchial biopsy, "humidifier lung" ...19958538084
induction, localization, and purification of a novel sialidase, deaminoneuraminidase (kdnase), from sphingobacterium multivorum.recently, we reported the discovery of a new type of sialidase, kdnase, which specifically hydrolyzes the ketosidic linkages of 2-keto-3-deoxy-d-glycero-d-galacto-nononic acid (kdn), but not n-acylneuraminyl linkages. we now report that this enzyme, designated kdnase sm, is an inducible enzyme that is localized in the periplasm of sphingobacterium multivorum. growth of s. multivorum in the presence of kdn-containing oligosaccharide alditols, kdnalpha2-->3galbeta1-->3galnac alpha1-->3[kdnalpha2-- ...19968621679
sphingobacterium multivorum septicemia: a case report.a 47-year-old man presented with a history of fever, chills and weight loss for 3 months. he had been treated for diabetes mellitus during the past 3 years. he developed high fever with abnormal liver function tests. both widal and weil-felix reactions were negative with normal roentgenogram of the chest. his anti-hiv tests were positive. the cultures from the blood and sputum yielded pure sphingobacterium multivorum sensitive to sulfamethoxazole-trimethoprim, chloramphenicol, tetracycline, cefo ...19968855615
catalysis by a new sialidase, deaminoneuraminic acid residue-cleaving enzyme (kdnase sm), initially forms a less stable alpha-anomer of 3-deoxy-d-glycero-d-galacto-nonulosonic acid and is strongly inhibited by the transition state analogue, 2-deoxy-2, 3-didehydro-d-glycero-d-galacto-2-nonulopyranosonic acid, but not by 2-deoxy-2,3-didehydro-n-acetylneuraminic acid.deaminoneuraminic acid residue-cleaving enzyme (kdnase sm) is a new sialidase that has been induced and purified from sphingobacterium multivorum. catalysis by this new sialidase has been studied by enzyme kinetics and 1h nmr spectroscopy. vmax/km values determined for synthetic and natural substrates of kdnase sm reveal that 4-methylumbelliferyl-kdn (kdnalpha2meumb, vmax/km = 0.033 min-1) is the best substrate for this sialidase, presumably because of its good leaving group properties. the tran ...19979038146
hydrolysis of imipenem, meropenem, ceftazidime, and cefepime by multiresistant nosocomial strains of sphingobacterium multivorum. 19979105852
[the effect of cefepime on ceftazidime, cefotaxime and imipenem resistant strains of acinetobacter, xanthomonas, pseudomonas, flavobacterium, sphingobacterium and on producers of extended spectrum beta-lactamases (esbl) with resistance transfer].the new cephalosporins with heterocyclic positively charged substituent at position c3 belong to the 4th generation cephalosporins. it is not evident, whereas these antibiotics are effective against cefotaxime-ceftazidime-resistant bacteria. we investigated the efficiency of the cefepime (maxipime, bms) against acinetobacter sp., x. maltophilia, pseudomonas sp., flavobacterium sp., sphingobacterium sp. and against strains with production of esbl (extended spectrum beta-lactamases) (enterobacter ...19979221190
induction of kdnase sm, a deaminoneuraminic acid (kdn) residue-specific sialidase from sphingobacterium multivorum, using synthetic kdn-glycosides.various aryl and alkyl alpha-glycosides of kdn were synthesized and tested as substrates for their susceptibility to a deaminoneuraminic acid (kdn)-specific sialidase from sphingobacterium multivorum, designated kdnase sm. the synthetic kdn-glycosides were all hydrolyzed by the action of kdnase sm. a hydroxyl group at c-5 position of kdn was required for the recognition by the enzyme, and was shown not to be replaced by an amino- or an acylamino group for the enzymatic recognition. these synthet ...19989703955
cloning, functional expression and purification of endo-beta-galactosidase from flavobacterium keratolyticus.endo-beta-galactosidase (ec is an enzyme that hydrolyzes internal endo-beta-galactosyl linkages in keratan sulfate, and glycoconjugates with n-acetyl-lactosamine repeating units. here, we report the cloning of the endo-beta-galactosidase-encoding gene from flavobacterium keratolyticus, its expression in escherichia coli and the purification of the enzyme. the enzyme was purified over 15000-fold to apparent homogeneity. the purified endo-beta-galactosidase consists of a single band of ...19989831650
flavobacterium spp. organisms as opportunistic bacterial pathogens during advanced hiv assess the role of flavobacterium spp. infection in patients with hiv disease.199910609533
molecular cloning of endo-beta -galactosidase c and its application in removing alpha -galactosyl xenoantigen from blood vessels in the pig kidney.galalpha1-3gal is the major xenoantigenic epitope responsible for hyperacute rejection upon pig to human xenotransplantation. endo-beta-galactosidase c from clostridium perfringens destroys the antigenic epitope by cleaving the beta-galactosidic linkage in the galalpha1-3galbeta1-4glcnac structure. based on partial peptide sequences of the enzyme, we molecularly cloned the enzyme gene, which encodes a protein with a predicted molecular mass of about 93 kda. the deduced protein sequence of the en ...200010858461
carotenoids of an antarctic psychrotolerant bacterium, sphingobacterium antarcticus, and a mesophilic bacterium, sphingobacterium multivorum.the major carotenoid pigments of an antarctic psychrotolerant bacterium, sphingobacterium antarcticus, and a mesophilic bacterium, sphingobacterium multivorum, were identified as zeaxanthin, beta-cryptoxanthin, and beta-carotene. analysis was based on ultraviolet-visible spectroscopy, mass spectroscopy, and reversed-phase hplc. photoacoustic spectroscopy of intact bacterial cells revealed that the bulk of the pigments in s. antarcticus and s. multivorum was associated with the cell membrane. in ...200010896223
zeaxanthin and menaquinone-7 biosynthesis in sphingobacterium multivorum via the methylerythritol phosphate pathway.feeding of [1-(13)c]glucose, [u-(13)c(6)]glucose, [3-(13)c]alanine and [1-(13)c]acetate to sphingobacterium multivorum showed that this bacterium utilizes the methylerythritol phosphate pathway for the biosynthesis of menaquinone-7 and zeaxanthin, a carotenoid of industrial importance. differential incorporation of the labeled precursors gave some insight into the preferred carbon sources involved in isoprenoid biosynthesis.200111731147
[respiratory infection caused by sphingobacterium multivorum]. 200111852504
effect of dietary zeaxanthin on tissue distribution of zeaxanthin and lutein in quail.the xanthophyll carotenoids (lutein and zeaxanthin) are hypothesized to delay progression of age-related macular degeneration. the quail has a cone-dominant retina that accumulates carotenoids. the purpose of these experiments was to characterize the carotenoid composition of retina, serum, liver, and fat in quail and to determine whether dietary enrichment with zeaxanthin alters zeaxanthin or lutein concentrations in these tissues.200211923268
occurrence of epsilon-poly-l-lysine-degrading enzyme in epsilon-poly-l-lysine-tolerant sphingobacterium multivorum oj10: purification and characterization.epsilon-poly-l-lysine (epsilon-pl)-degrading enzyme was found in the epsilon-pl-tolerant strain sphingobacterium multivorum oj10 and purified to homogeneity. the purified enzyme has a molecular mass of approximately 80 kda. the enzyme catalyzed exo-type degradation of epsilon-pl and released l-lysine. the enzyme was a co2+ or ca2+ ion-activated aminopeptidase.200211958932
identification of non-pseudomonad bacteria from fruit bodies of wild agaricales fungi that detoxify tolaasin produced by pseudomonas tolaasii.bacterial isolates from wild agaricales fungi detoxified tolaasin, the inducer of brown blotch disease of cultivated mushrooms produced by pseudomonas tolaasii. mycetocola tolaasinivorans and mycetocola lacteus were associated with fruit bodies of wild pleurotus ostreatus and wild lepista nuda, respectively. tolaasin-detoxifying bacteria belonging to other genera were found in various wild mushrooms. an acinetobacter sp. was isolated from fruit bodies of tricholoma matsutake, bacillus pumilus wa ...200212450133
resonant raman quantification of zeaxanthin production from flavobacterium multivorum.resonant raman scattering was used as a novel, rapid, non-destructive optical technique to measure zeaxanthin levels in flavobacterium multivorum atcc 55238. culture broth, after bacterial growth for 40 h, exhibited characteristic resonance raman vibrational modes at 1159 cm(-1) (c-c stretch) and 1525 cm(-1) (c=c stretch) upon excitation at 488 nm. a striking correlation was observed between the carotenoid level as estimated by hplc and by resonance raman spectroscopy.200312889806
identification of novel eubacteria from spent mushroom compost (smc) waste by dna sequence typing: ecological considerations of disposal on agricultural land.a small study was undertaken to examine the microbiological characteristics of spent mushroom compost (smc), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. the primary aim of this study was to examine smc for the presence of faecal bacterial pathogens, including campylobacter spp., salmonella spp. and listeria monocytogenes. secondly it was desirable to quantify bacterial and fungal populations within smc, and ...200414672727
structural analysis of sphingophospholipids derived from sphingobacterium spiritivorum, the type species of genus sphingobacterium.the unique feature of the genus sphingobacterium is the presence of sphingophospholipids and ceramides, besides diacylglycerophospholipids. as major cellular lipid components, five kinds of sphingophospholipids were purified from sphingobacterium spiritivorum atcc 33861(t), the type species of genus sphingobacterium. they were identified as ceramide phosphorylethanolamines (cerpe-1 and cerpe-2), ceramide phosphoryl-myo-inositols (cerpi-1 and cerpi-2), and ceramide phosphorylmannose (cerpm-1). th ...200314729071
role of bacteria in mediating the oviposition responses of aedes albopictus (diptera: culicidae).the responses of aedes albopictus to sources of oviposition attractants and stimulants were evaluated with a behavioral bioassay in which females attracted to odorants emanating from water were trapped on screens coated with an adhesive. gravid mosquitoes were attracted to volatiles from larval-rearing water and soil-contaminated cotton towels. bacteria were isolated from these substrates and from an organic infusion made with oak leaves. through fatty acid-methyl ester analyses, six bacterial i ...200314765661
factorial analysis of tricarboxylic acid cycle intermediates for optimization of zeaxanthin production from flavobacterium study the effect of intermediates of the tricarboxylic acid (tca) cycle on the production of zeaxanthin from flavobacterium multivorum in order to optimize production of this xanthophyll carotenoid.200414962143
beta-carotene production by flavobacterium multivorum in the presence of inorganic salts and urea.flavobacterium multivorum, a non-fermenting gram-negative bacteria, normally produces zeaxanthin (3r, 3' r-beta, beta-carotene-3, 3' diol) as its main carotenoid. the effect of supplementation of various inorganic salts and urea on the growth, total carotenoid production, and proportion of beta-carotene (beta, beta-carotene), beta-cryptoxanthin (beta, beta-caroten-3-ol), and zeaxanthin produced by f. multivorum was investigated. urea and several salts, such as calcium chloride, ammonium chloride ...200415592945
['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp].gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ...200515991478
production of deuterated zeaxanthin by flavobacterium multivorum and its detection by resonance raman and mass spectrometric methods.flavobacterium multivorum, a zeaxanthin-producing organism, was grown aerobically in a medium prepared with deuterated water. atmospheric pressure chemical ionization mass spectrometry (apci-ms) and resonance raman spectroscopy (rrs) analysis revealed approximately 75% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 600. deuterated zeaxanthin upon excitation with a 488 nm laser exhibited characteristic resonance raman vibrational modes at 1,161 ...200516247681
isolation and characterization of bacterial strains with inulinase activity.thirty-two bacterial strains growing on inulin as the sole carbon and energy source were isolated from soil samples by enrichment culture on a mineral medium. twenty of the strains were identified as flavobacterium multivorum. all the bacteria contained a beta-fructosidase that was active on both inulin and sucrose. the enzyme activity was cell bound and was produced at the end of the growth phase. these enzymes have potential uses in the preparation of fructose syrups from inulin and invert sug ...198616347206
evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ...200716822636
molecular characterization of membrane-associated soluble serine palmitoyltransferases from sphingobacterium multivorum and bdellovibrio stolpii.serine palmitoyltransferase (spt) is a key enzyme in sphingolipid biosynthesis and catalyzes the decarboxylative condensation of l-serine and palmitoyl coenzyme a (coa) to form 3-ketodihydrosphingosine (kds). eukaryotic spts comprise tightly membrane-associated heterodimers belonging to the pyridoxal 5'-phosphate (plp)-dependent alpha-oxamine synthase family. sphingomonas paucimobilis, a sphingolipid-containing bacterium, contains an abundant water-soluble homodimeric spt of the same family (h. ...200717557831
sphingobacterium canadense sp. nov., an isolate from corn roots.a free-living gram-negative bacterial strain cr11(t) was isolated from corn roots. polyphasic taxonomy was performed, including api20 ne and api50 ch bacterial identification kits, biolog analysis, lipids and fatty acid analysis, dna-dna hybridization, 16s rrna and cpn60 gene sequence analyses. 16s rrna gene sequence analysis indicated that strain cr11(t) belonged to the genus sphingobacterium and was closely related to sphingobacterium multivorum ifo 14947(t) (98% similarity) and sphingobacteri ...200717629434
conversion of lesquerolic acid to 14-oxo-11(z)-eicosenoic acid by genetically variable sphingobacterium multivorum strains.we investigated new microbial systems for their ability to convert lesquerolic acid (lqa; 14-hydroxy-11(z)-eicosenoic acid) to value-added products. a strain of sphingobacterium multivorum (nrrl b-23212) was found previously to convert lqa to 14-oxo-11(z)-eicosenoic acid (14-oea) as determined by gas chromatography-mass spectrometry and nuclear magnetic resonance analyses. conversion of lqa was subsequently extended to examine s. multivorum and closely related species of pedobacter, spirosoma, c ...200818379841
sphingobacterium siyangense sp. nov., isolated from farm soil.the taxonomic position of a novel gram-negative strain, designated sy1(t), isolated from a farm-soil sample obtained from jiangsu province, pr china, was characterized by using a polyphasic approach. the cells were non-motile, non-spore-forming rods. the organism grew optimally at 30-37 degrees c and at ph 6.0-8.0. based on 16s rrna gene sequence analysis, strain sy1(t) is a member of the genus sphingobacterium; sphingobacterium multivorum jcm 21156(t) was the nearest relative (98.5 % sequence s ...200818523194
structural insights into the enzymatic mechanism of serine palmitoyltransferase from sphingobacterium multivorum.serine palmitoyltransferase (spt) is a key enzyme of sphingolipid biosynthesis and catalyses the pyridoxal 5'-phosphate (plp)-dependent decarboxylative condensation reaction of l-serine with palmitoyl-coa to generate 3-ketodihydrosphingosine. the crystal structure of spt from sphingobacterium multivorum gtc97 complexed with l-serine was determined at 2.3 a resolution. the electron density map showed the schiff base formation between l-serine and plp in the crystal. because of the hydrogen bond f ...200919564159
influence of tetracycline exposure on the growth of wheat seedlings and the rhizosphere microbial community structure in hydroponic this study, the effects of tetracycline exposure on wheat growth and the microbial community structure in the rhizosphere were investigated under hydroponic culture conditions. exposure to various concentrations of tetracycline resulted in significant suppression of the growth of wheat roots and shoots, with minimum doses of 0.8 mg l(-1) and 4 mg l(-1) resulting in inhibition rates of 32% and 15.4%, respectively. complete inhibition of the growth of these two parts of wheat plants was observe ...201020390950
Necrotizing Fasciitis and Septic Shock Related to the Uncommon Gram-Negative Pathogen Sphingobacterium multivorum.We report the first case of necrotizing fasciitis due to the uncommon Gram-negative pathogen Sphingobacterium multivorum in an immunocompromised patient, who presented with septic shock. This case adds necrotizing fasciitis to the spectrum of S. multivorum-related infections and highlights the emergence of Gram-negative bacteria in severe soft tissue infections.201222075581
identification of soil microbes capable of utilizing cellobiosan.approximately 100 million tons of anhydrosugars, such as levoglucosan and cellobiosan, are produced through biomass burning every year. these sugars are also produced through fast pyrolysis, the controlled thermal depolymerization of biomass. while the microbial pathways associated with levoglucosan utilization have been characterized, there is little known about cellobiosan utilization. here we describe the isolation and characterization of six cellobiosan-utilizing microbes from soil samples. ...201626872347
assessing the xylanolytic bacterial diversity during the malting process.the presence of microorganisms producing cell wall hydrolyzing enzymes such as xylanases during malting can improve mash filtration behavior and consequently have potential for more efficient wort production. in this study, the xylanolytic bacterial community during malting was assessed by isolation and cultivation on growth media containing arabinoxylan, and identification by 16s rrna gene sequencing. a total of 33 species-level operational taxonomic units (otus) were found, taking into account ...201324010623
preparation of petroleum-degrading bacterial agent and its application in remediation of contaminated soil in shengli oil field, china.two petroleum-degrading strains were screened from oil fields and denoted as swh-1 (bacillus subtilis) and swh-2 (sphingobacterium multivorum), which were used to ferment and prepare bacterial agent to remediate petroleum-contaminated sites in shengli oil field in china. the optimal liquid fermentation medium and conditions were mgso₄·7h₂o (0.5%), nacl (0.5%), soybean dregs (3%), ph 7.0, culturing at 30 °c, and 220 r/min for 16 h. peat was chosen as the bacterial carrier due to its ability of ke ...201424652573
transformation of microflora during degradation of gaseous toluene in a biofilter detected using pcr-dgge.a laboratory-scale biofiltration system, the rotatory-switching biofilter (rsb), was operated for 199 days using toluene as a model pollutant. the target gaseous pollutant for the biofiltration experiment was approximately 300 ppmv of toluene. toluene removal efficiency (re, %) was initially approximately 20% with a 247-ppmv concentration (0.9 g m(-3)) of toluene during the first 10 days. although the re decreased several times whenever nitrogen was consumed, it again reached almost 100% when th ...201222866576
characterization of smart auto-degradative hydrogel matrix containing alginate lyase to enhance levofloxacin delivery against bacterial biofilms.the aim of the present work is the characterization of smart auto-degradable microspheres composed of calcium alginate/high methoxylated pectin containing an alginate lyase (al) from sphingobacterium multivorum and levofloxacin. microspheres were prepared by ionotropic gelation containing al in its inactive form at ph 4.0. incubation of microspheres in tris-hcl and pbs buffers at ph 7.40 allowed to establish the effect of ion-chelating phosphate on matrix erodability and suggested an intrinsical ...201526505149
sphingobacterium multivorum bacteremia and acute meningitis in an immunocompetent adult patient: a case report.sphingobacterium multivorum is a gram-negative, nonfermentative bacillus that rarely causes disease in humans. in the medical literature, only a few cases of infections caused by this organism have been reported. almost all the reported cases of this infection were associated with conditions that decrease immunity.201628144466
sphingobacterium zeae sp. nov. an endophyte of maize.a yellow pigmented strain (jm-1081t) isolated from healthy stem tissue of zea mays was taxonomically characterized. cells of the strain appeared rod-shaped and stained gram-negative. comparative 16s rrna gene sequence analysis revealed closest relationship to the type strains of sphingobacterium multivorum (98.1%), sphingobacerium mucilaginosum (97.9%), and sphingobacterium siyangense (97.8%). the 16s rrna gene sequence similarities to all other sphingobacterium species were below 97.8%. fatty a ...201627098598
septic arthritis by sphingobacterium multivorum in imunocompromised pediatric report a case septic arthritis with a rare pathogen in a immunosuppressed child.201626915918
sphingobacterium multivorum: case report and literature review.we describe the case of a 67-year-old african american woman with multiple medical problems who presented with septic shock resulting from sphingobacterium multivorum bacteraemia. s. multivorum, a gram-negative bacillus, is ubiquitous in nature and is rarely involved in human infections. however, it is intrinsically resistant to many commonly administered antibiotics and can be a life-threatening microorganism.201526236492
sphingobacterium mucilaginosum sp. nov., isolated from rhizosphere soil of a rose.a gram-stain-negative, strictly aerobic, motile, short-rod-shaped bacterium, designated strain thg-sqa8(t), was isolated from rhizosphere soil of rose in pr china. strain thg-sqa8(t) was closely related to members of the genus sphingobacterium, showed the highest sequence similarities with sphingobacterium multivorum kacc 14105(t) (98.0%) and sphingobacterium ginsenosidimutans kacc 14526(t) (97.4%). dna-dna hybridization showed values of 35.2 ± 0.9% and 8.8 ± 0.3% dna reassociation with s. multi ...201526023207
bioflocculation behaviours of microbial communities in water treatment.we studied the flocculation behaviours of microbial communities in 21 soil, wastewater and activated sludge samples to clarify the effects of culture medium types on flocculation ability and screening efficiency, and to analyze diverse functions and microbial compositions. the bioflocculants produced by 33% of the microbial communities had flocculating efficiencies higher than 90%. six out of the eight microbial communities with efficiencies over 94% were screened from the culture medium using d ...201424569266
sphingobacterium changzhouense sp. nov., a bacterium isolated from a rice field.a gram-stain-negative, yellow, non-spore-forming, rod-shaped bacterium, designated n7(t), was isolated from a soil sample collected from a rice field in jiangsu, china, and was characterized using a polyphasic taxonomic approach. strain n7(t) grew optimally at 25-30 °c, ph 6.0-8.0, and in the presence of 1 % nacl (w/v). 16s rrna gene sequence analysis indicated that strain n7(t) was a member of the genus sphingobacterium and was closely related to sphingobacterium multivorum iam14316(t) (97.49 % ...201323859944
inoculation of sphingobacterium multivorum in the prostate by prostate biopsy.this report describes three cases of infection with sphingobacterium multivorum after transrectal ultrasound-guided prostate biopsy. the pathogen is ubiquitous in water and soil but has been described fewer than 10 times causing infections in humans. an infection hygiene evaluation identified and changed a step in the biopsy process in order to reduce the risk of inoculating the patient with environmental microorganisms.201423834407
production of ginsenoside aglycons and rb1 deglycosylation pathway profiling by hplc and esi-ms/ms using sphingobacterium multivorum gin723.using enrichment culture, sphingobacterium multivorum gin723 (kccm80060) was isolated as having activity for deglycosylation of compound k and ginsenoside f1 to produce ginsenoside aglycons such as s-protopanaxadiol (ppd(s)) and s-protopanaxatriol (ppt(s)). through blast search, purified enzyme from s. multivorum gin723 was revealed to be the outer membrane protein. the purified enzyme from s. multivorum gin723 has unique specificity for the glucose moiety. however, it has activity with ppd and ...201323812276
sphingobacterium caeni sp. nov., isolated from activated sludge.the taxonomic status of a bacterium, strain dc-8(t), isolated from activated sludge, was determined using a polyphasic taxonomic approach. the cells of strain dc-8(t) were gram-negative, non-motile, non-spore-forming and rod-shaped. the isolate grew at temperature range of 10-40 °c (optimum 30-35 °c), ph range of 5.0-10.0 (optimum 6.5-8.0) and nacl concentrations of 0-5% (optimum 0-1%). the predominant menaquinone of strain dc-8(t) was mk-7 and major fatty acids were summed feature 3 (c16:1ω6c a ...201323148100
sphingobacterium cladoniae sp. nov., isolated from lichen, cladonia sp., and emended description of sphingobacterium siyangense.a strictly aerobic, gram-stain-negative bacterium, designated strain no.6(t), was isolated from a lichen (cladonia sp.) collected in geogeum island, korea, and its taxonomic status was established by a polyphasic study. cells of strain no.6(t) were non-motile, catalase- and oxidase-positive, non-spore-forming rods. growth was observed at 15-35 °c (optimum, 25-30 °c), at ph 5.0-10.0 (optimum, ph 6.0-8.0) and with 0-3 % nacl (optimum, 0-2 %). the predominant cellular fatty acids were summed featur ...201322561594
sphingobacterium detergens sp. nov., a surfactant-producing bacterium isolated from soil.a novel gram-negative-staining strain, designated 6.2s(t), was isolated from a soil sample and identified as a biosurfactant producer. its taxonomic position was investigated using a polyphasic approach. the cells were non-motile, non-spore-forming rods. the organism grew optimally at 30-37 °c, with 0-3% (w/v) nacl, and at ph 7.0. based on 16s rrna gene sequence analysis, strain 6.2s(t) was found to be a member of the genus sphingobacterium and was most closely related to four type species of th ...201222307508
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