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autolysis in strains of viridans streptococci.seven strains of viridans streptococci of the species streptococcus sanguis, s. mutans and s. mitis were investigated for autolysis. the effect of ph, salt concentration and temperature on the autolytic process was studied in na2hpo4/nah2po4 buffer. whole cells and walls of all strains autolysed most rapidly at ph values above 7. autolysis of whole cells of s. sanguis and one strain of s. mitis (atcc15909) was maximal in 0-05 to 0-2 m buffer, while the two s. mutans strains and s. mitis atcc1591 ...197610349
extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilue acid.autoclaving cell wall of streptococcus mutans ingbritt for 15 min under the rantz and randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. the extract contained the serotype c-specific antigen but lacked the lipoteichoic acid component extracted when whole cells were autoclaved. autoclaving cell wall preparations from other strains of s. mutans and also streptococcus salivarius and streptococcus mitis in 0.85% ...197832140
[phlegmon of the fore arm succeeding to a dog-bite : respective role of the miscellaneous bacteria involved in this mixed infection (author's transl)].the authors report a case of a severe mixed infection (phlegmon of the fore arm) succeeding to a dog bite. a complex flora has been isolated from this phlegmom : pasteurella multocida, pasteurella pneumotropica, streptococcus mitis, actinomyces sp., bacteroides melaninogenicus, and a gram- bacteria related to group ii j. the pathogeny of such diseases is discussed, dwelling in one hand on the part of synergistic development of these various germs and in the other hand on the part played by saliv ...197934886
purification and characterization of beta-fructofuranosidase (invertase) from an oral strain of streptococcus mitis. 197943121
interactions between neisseria sicca and viridin b, a bacteriocin produced by streptococcus mitis.viridin b, a bacteriocin produced by streptococcus mitis (mitior), is bactericidal to neisseria sicca. oxygen consumption by actively growing n. sicca cultures ceased immediately upon exposure to viridin b. adenosine triphosphate production was slightly enhanced within 1 h of exposure to the bacteriocin but was subsequently repressed. the uptake and incorporation of glucose was prevented in the presence of viridin b. the bacteriocin also blocked uptake of an amino acid mixture in chloramphenicol ...1978263887
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
experimental transient bacteraemias in human subjects with clinically healthy gingivae.thirty-six subjects with no evidence of clinical gingival inflammation underwent a standardized toothbrushing procedure. blood specimens, obtained from a vein in the antecubital fossa during the last 30 seconds of brushing, were cultured under aerobic and stringent anaerobic conditions. three subjects exhibited detectable bacteraemias, propionibacterium sp. being isolated from two of the subjects, while actinomyces sp., streptococcus sanguis and streptococcus mitis were isolated from the third. ...1979285080
isoelectric focusing studies on the beta-fructofuranosidases and alpha-glucosidases of streptococcus mitis.extracts of streptococcus mitis atcc 903 were analysed for beta-fructofuranosidase and alpha-glucosidase activities by isoelectric focusing in thin-layer polyacrylamide gels combined with zymogram procedures. three bands of activity were visualized in the gels after incubation with sucrose (pi 4.05, 4.25 and 4.85) and three other bands after incubation with p-nitrophenyl alpha-d-glucopyranoside (pi 3.90, 4.45 and 4.65). the enzymes responsible for the reaction with sucrose were identified as bet ...1978353225
rat model of experimental endocarditis.a simple model of infective endocarditis was produced in rats. with the aid of a guide wire, polyethylene catheters were passed into the left ventricle through the right carotid artery of sprague-dawley rats weighing 300 to 350 g. a volume of 1 ml of an overnight culture of streptococcus mitis, staphylococcus aureus, or streptococcus faecalis was intravenously injected 1 to 2 days after catheterization. bacterial titers of streptococcus mitis in vegetations were about 10(4)-fold greater than in ...1978417032
serologic studies of streptococcus intermedius, streptococcus constellatus, and streptococcus morbillorum by crossed immunoelectrophoresis.a reference antigen-antibody system for streptococcus intermedius, streptococcus constellatus, and streptococcus morbillorum was established with crossed immunoelectrophoresis. a comparison of s. intermedius, s. constellatus, and s. morbillorum with crossed immunoelectrophoresis and crossed immunoelectrophoresis with intermediate gel indicated that s. intermedius and s. constellatus are closely related antigenically with as many as six common cytoplasmic antigens. s. morbillorum was antigenicall ...1979453098
purification of a streptococcal bacteriocin (viridin b) and its separation from alpha-hemolysin.viridin b, a bacteriocin produced by streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on sephadex g-200. the bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-sephadex a-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutag ...1979464570
chemical composition of streptococcus mutans cell walls and their susceptibility to flavobacterium l-11 enzyme.the susceptibility to a cell wall lytic l-11 enzyme from flavobacterium sp. and the quantitative and/or qualitative composition of the cell walls of some strains of cariogenic streptococcus mutans and a non-cariogenic strain of streptococcus mitis were determined. the purified cell walls of s. mutans strains hs-1 (serotype a), bht (b), nctc10449 (c), c67-1 (c), c67-25 (c), omz 176 (d), mt703 (e), mt557 (f), omz65 (g), and aht (g), and s. mitis cht contained glutamic acid, alanine, and lysine as ...1979502897
inhibitory activity of streptococcus mitis against oral bacteria.the antagonistic properties of three strains of streptococcus mitis were investigated. they were found to inhibit a wide range of oral bacteria; gram-positive and gram-negative, facultative and anaerobic species being susceptible. the s. mitis strains were shown to be producing hydrogen peroxide, this being partially responsible for the aerobic inhibitory activity. a second inhibitory factor(s) was also produced, aerobically and anaerobically, although this could not be isolated. a limited chara ...1979551241
experimental streptococcal endocarditis. the early vegetation.aortic valve vegetation were studied in rabbits before and after intravenous injection of streptococcus mitis. uninfected vegetation sections revealed compact eosinophilic masses with smooth-appearing edges and scattered polymorphonuclear leukocytes inside and on the surface. scanning electron microscopic views demonstrated erythrocytes, small leukocytes, and platelets; the surface was markedly irregular. fifteen minutes after injection of 10(10) streptococci, large mononuclear cells and polymor ...1978581340
adherence of veillonella species mediated by extracellular glucosyltransferase from streptococcus salivarius.the effect of extracellular products from streptococcus salivarius on sucrose-dependent adherence to smooth surfaces by other oral bacteria was studied in vitro. strains of streptococcus mitis, streptococcus pyogenes, and veillonella parvula without innate ability to adhere to a steel wire were able to do so when incubated with sucrose and cell-free culture fluid from s. salivarius strains 9759, 25975, cnii, and mepi. these culture fluids synthesized more adherent material and water-insoluble gl ...1977591064
substances that interfere with action of viridin b, a streptococcus mitis bacteriocin.several body fluids and various substances were examined for their effects on the action of viridin b, a streptococcus mitis bacteriocin. heme has been shown previously to interfere with viridin b action. in the present study, peroxidase and catalase, but not superoxide dismutase, interfered with the bacteriocin. sulfhydryl-containing compounds had no effect. trypsin, protease, and amylase exhibited interfering capacities. of various body fluids tested, serum, saliva, and leukocyte lysate interf ...1978669792
absence of glycerol teichoic acids in certain oral streptococci.glycerol teichoic acids were not detected immunochemically or chemically in phenol-water, hot saline (rantz and randall), or supernatant fluids of disrupted cells of streptococcus mitis. thus teichoic acids do not appear to be found in most gram-positive bacteria, as has been suggested.1978684416
determinants of the developing oral flora in normal newborns.the ability of streptococcus species to selectively adhere to the oral epithelial cells of newborns was studied in vitro. on day 1 of life, mucosal cells from normal infants demonstrated selective attraction for the natural distribution of streptococci that would soon colonize these surfaces. streptococcus salivarius and streptococcus mitis adhered well in vitro to scraped cells from cheek and tongue surfaces. streptococcus mutans, on the other hand, exhibited feeble or no adherence to cheek or ...1976791120
origin of esterases in human whole saliva.whole saliva of 59 healthy persons was used for determination of esterase activity. the pattern of esterase was studied by means of isoelectrofocusing on thin-layer acrylamide gels. the esterases found in whole saliva are suggested to be derived from the cells of the tissue in the oral cavity. this origin is indicated (e.g.) by comparison between isolectrophoretic esterase patterns of whole saliva, submandibular saliva, gingival biopsy and fibroblast culture. antisera against partially purified ...1977862601
urease assay and urease-producing species of anaerobes in the bovine rumen and human feces.a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ...1977879770
influence of salivary components and extracellular polysaccharide synthesis from sucrose on the attachment of streptococcus mutans 6715 to hydroxyapatite surfaces.the adsorption of (3)h-labeled streptococcus mutans 6715 cells to disks of hydroxyapatite (ha) was studied. the number of streptococci that adsorbed was logarithmically related to the concentration of cells available up to at least 2 x 10(8) per ml; equilibrium occurred within 45 min. assay reliability was verified by direct scanning electron microscopic counts. untreated ha disks exposed to buffered saline (pbs)-suspended streptococci at a concentration of 1.1 x 10(8) per ml absorbed 3.2 x 10(6 ...1977924680
ultrastructural and biochemical alterations effected by viridin b, a bacterocin of alpha-hemolytic streptococci.viridin b, a bacteriocin produced by streptococcus mitis strain 42885, was shown previously to exert a bactericidal effect against a neisseria sicca strain but only a bacteriostatic effect against a coagulase-negative staphylococcus. in the present report, this dual effect was defined further. viridin b caused the appearance of mesosomes in both indicator strains early in the course of exposure. whereas mesosomes were the only noticeable alterations in the staphylococcal strain at all times of e ...1976965095
streptococcus salivarius, streptococcus sanguis and streptococcus "mitis" as indicators of contamination of root canal cultures.small samples were taken from the first drop of saliva entering an operation field for root canal treatment isolated by rubber dam, which was intentionally leaky. the samples were primarly cultured in tas- and blh-medium and on ms-agar aerobically. the culture media were inoculated directly and via transport medium (vmg iii). the cultures were analysed for strep. salivarius, strep. sanguis and/or strep. "mitis" after subculture on ms-agar as soon as visible growth had appeared in the broth media ...19751057108
bactericidal activity of combinations of penicillin or clindamycin with gentamicin or streptomycin against species of viridans streptococci.checkerboard bactericidal studies were performed with two strains each of streptococcus mitis, s. salivarius, and s. sanguis, and the results were analyzed by the use of isobolograms. synergy between penicillin and streptomycin or gentamicin was demonstrated with four strains, representing two of s. salivarius, one of s. sanguis, and one of s. mitis; the remainder were either too susceptible or too resistant to be analyzed. clindamycin with streptomycin or gentamicin acted synergistically with t ...19751211918
serum antibody responses to streptococcus mutans antigens in humans systemically infected with oral streptococci.sera from patients with subacute bacterial endocarditis (sbe) due to streptococcus mutans or other oral streptococci and from normal subjects were assayed by enzyme-linked immunosorbent assay for antibodies to defined s. mutans antigens. antibodies of igg and iga isotypes to ag i/ii and ag iii were greatly elevated in s. mutans-sbe sera, and the iga antibodies in 3 sera included both polymeric and monomeric forms. elevated igm and igg anti-lipoteichoic acid and igg and iga anti-serotype c polysa ...19921299798
immunoglobulin a antibody activity to mutans streptococci in parotid, submandibular and whole saliva.antigen extracts of 9 strains of streptococcus mutans, streptococcus sobrinus, streptococcus salivarius and streptococcus mitis were separated in acrylamide gels by electrophoresis. strains of the same genotype of mutans streptococci were found to have virtually identical sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns but differed clearly from other genotypes of mutans or with the reference strains s. salivarius and s. mitis. immunoglobulin a (iga) antibody activity to antige ...19921299799
quantitation of cytomegalovirus dna in lung tissue of bone marrow transplant recipients.five bone marrow transplant recipients who died of respiratory failure were retrospectively analyzed with polymerase chain reaction (pcr) assay for pulmonary cytomegalovirus (cmv) infection. two patients had cmv interstitial pneumonitis according to the virus isolation and the histologic and immunofluorescent examinations of the lungs, while the other three patients had non-cmv diseases (ie, idiopathic interstitial pneumonitis, pulmonary aspergillosis, or streptococcus mitis septicemia). cytomeg ...19921322861
characterization of the adherence of porphyromonas gingivalis to oral streptococci.adherence of porphyromonas gingivalis to strains of streptococcus sanguis and streptococcus mitis deposited on nitrocellulose paper was investigated. a variety of laboratory strains and clinical isolates of p. gingivalis bound to both s. sanguis and s. mitis. binding of p. gingivalis to all but one of the streptococci was not inhibited by salivary molecules. pretreatment of p. gingivalis with periodate and pretreatment of s. sanguis and s. mitis with pronase decreased binding, suggesting that ad ...19921328995
restriction endonuclease-fragment polymorphisms of oral viridans streptococci, compared by conventional and field-inversion gel electrophoresis.oral streptococci formerly classified as streptococcus sanguis or streptococcus mitis have recently been divided into four species. two additional species have also been proposed for this group. each species is genetically distinct, but they have many traits in common, which makes it difficult for clinical isolates to be identified by phenotypic tests. genotypic comparison may provide an alternative approach. this study used dna fingerprint analysis for comparison of genotypes of 21 reference st ...19921351484
purification and characterization of an aminopeptidase from streptococcus mitis atcc 903.an aminopeptidase isolated from the cytoplasmic fraction of a cell extract of streptococcus mitis atcc 903 was purified 330-fold by ion-exchange chromatography, gel filtration, and hydroxyapatite chromatography. the partially purified enzyme had a broad substrate specificity. twelve aminoacyl-beta-naphthylamide substrates were hydrolyzed and also several di-, tri-, tetra-, and pentapeptides and bradykinin. the enzyme hydrolyzed arginine-beta-naphthylamide at the highest rate. optimal conditions ...19921369197
enzymic activity of salivary amylase when bound to the surface of oral streptococci.the enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. most strains able to bind amylase exhibited functional enzyme on their surface and produced acid from the products of amylolytic degradation. these strains were unable to utilise starch in the absence of salivary amylase. two strains failed to produce ...19921376290
identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases.activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ...19921398963
purification and properties of sorbitol-6-phosphate dehydrogenase from oral streptococci.the activity of sorbitol-6-phosphate (s6p) dehydrogenase (s6pdh) and the sorbitol transport system were studied in strains of the oral streptococci streptococcus gordonii, streptococcus mitis, streptococcus sanguis and streptococcus mutans. genetically transformed (to ferment sorbitol) strains and their dna donors were included. s6pdh was purified by anion exchange chromatography and gel filtration. the purity of the enzyme was confirmed by polyacrylamide gel electrophoresis. the purified enzyme ...19921408350
infectious crystalline keratopathy with ring opacity.a 41-year-old physician was treated for 3 months with antiviral medications, antibiotics, and steroids for presumed herpetic keratitis. when seen by us, an annular infiltrate was observed, along with crystalline-like opacities in the superficial one third of the stroma. cultures of scrapings and of subsequent biopsies were positive for streptococcus mitis of the viridans group; histopathology demonstrated large aggregates of cocci between the stroma lamellae. tapering of topical corticosteroids ...19921424678
inhibition of microbial adherence by sphinganine.sphingosines (precursors and degeneration products of complex sphingolipids) are mediators in membrane second-messenger cascades and in a wide variety of functions in eukaryotic cells. sphingosines are also lethal for gram-positive microorganisms. in addition to its direct effect, sphinganine is here reported to affect the adherence of streptococcus mitis to buccal epithelial cells and of staphylococcus aureus to nasal mucosal cells after incubation for 90 min at 37 degrees c. when the bacteria ...19921464071
[supravalvular aortic stenosis and coronary aneurysm].a 32-year old woman, with endocarditis caused by streptococcus mitis, and systolic murmur is presented. the doppler examination was found a systolic gradient of 150 mmhg. aortography showed a multiple membranous supravalvular aortic stenosis, with aneurysmal dilatation of the left main coronary artery and circumflex artery, associated with bicuspid aortic valve and mild aortic insufficiency. the patient died suddenly by cardiac arrest in stand by to cardiac surgery. anatomic comprobation was not ...19921470747
physico-chemical and structural properties of the surfaces of peptostreptococcus micros and streptococcus mitis as compared to those of mutans streptococci, streptococcus sanguis and streptococcus salivarius.the surface properties of nine streptococcus mitis and four peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. in addition, elemental surface concentration ratios were obtained via x-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. the s. mitis and p. mi ...19921487734
in vitro antimicrobial susceptibility of viridans streptococci isolated from blood cultures.the susceptibility of 211 viridans streptococci isolated from blood cultures to eight antimicrobial agents was determined. all the isolates were susceptible to cefotaxime, ceftriaxone, imipenem and vancomycin. thirty eight percent of the isolates were resistant to penicillin (mics greater than or equal to 0.25 micrograms/ml). tetracycline resistance was found in 41% of the isolates and in 7% of these strains tetracycline resistance was combined with erythromycin resistance. five streptococcus mi ...19921526239
enumeration of subgingival species on primary isolation plates using colony lifts.this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ...19921528619
isolation and properties of the capnocytophaga ochracea bacteriocin.a bacteriocin from a subgingival plaque isolate of capnocytophaga ochracea was purified and characterized. it was isolated from cell extract and had a molecular weight of 100,000. streptococcus sanguis, streptococcus mitis, streptococcus mutans and propionibacterium acnes were susceptible to the bacteriocin.19921528638
streptococcal and enterococcal bacteremia in patients with cancer.eighty-two episodes of bacteremia due to streptococci (including the genus enterococcus) in 78 patients hospitalized at institut jules bordet between 1986 and 1988 were reviewed. the incidence ranged from 5.5 to 7.6 per 1,000 admissions (16% of all bacteremias). enterococcus faecalis, streptococcus sanguis, and streptococcus mitis were the most prevalent isolates, followed by streptococcus angionosus, streptococcus salivarius, and large colony-forming beta-hemolytic species (a, b, c, and g). twe ...19921617072
antimicrobial resistance in streptococcus pneumoniae: a south african perspective.resistance to penicillin among south african strains of streptococcus pneumoniae increased from 4.9% in 1979 to 14.4% in 1990. except for resistance to co-trimoxazole (44%), resistance to other antimicrobial agents remained relatively low. multiply resistant strains belonged mainly to serovars 6b, 19a, 14, and, more recently, 23f. use of chloramphenicol to treat meningitis caused by strains relatively resistant to penicillin proved to be unsatisfactory, probably because of the inadequate bacteri ...19921617077
septic complications of endoscopic laser treatment of gastrointestinal diseases.this study was designated to establish the incidence of septic complications associated with nd:yag-laser treatment of varied gastrointestinal lesions. a total of 30 laser procedures were performed for 27 patients with both upper and lower gastrointestinal diseases. thirty minutes after the completion of laser therapy, blood culture was obtained from each patient. only one patient developed sepsis with fever, chills and positive blood culture, the organism isolated was streptococcus mitis. all t ...19921622047
streptococcus mitis and ards in neutropenic patients. 19911671448
antibacterial and antifungal properties of propylene glycol, hexylene glycol, and 1,3-butylene glycol in vitro.the antimicrobial properties of three glycols, - propylene glycol, hexylene glycol, and 1,3-butylene glycol - against candida albicans, staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes a, streptococcus mitis, and e. coli were studied in vitro. within 20 h, 10% and 30% hexylene glycol in fresh tryptic soy broth were able to kill all the micro-organisms listed above. five percent hexylene glycol showed some antimicrobial properties but the 1% agent had no effect. thirty pe ...19911675525
characterization of infectious crystalline keratitis caused by a human isolate of streptococcus mitis.streptococcus mitis isolated from a human with infectious crystalline keratitis was injected intrastromally into corneas of adult new zealand white rabbits that were treated with tetracycline hydrochloride, methylprednisolone acetate, or a combination of tetracycline and methylprednisolone. animals were followed up for up to 44 days; untreated corneas and those treated with tetracycline developed no disease or "fluffy" stromal infiltrates with overlying epithelial defects representing an abscess ...19911714271
in vitro studies on the microbicidal effectiveness of a xenon-based ultraviolet light device for continuous ambulatory peritoneal dialysis connections.mercury vapor sourced ultraviolet light has been used for several years to disinfect connections that are integral to peritoneal dialysis solution delivery systems. this in vitro study evaluated the suitability of xenon flash sourced ultraviolet light as an effective alternative disinfecting agent. candida albicans was determined to be at least four times more resistant than staphylococcus epidermidis, staphylococcus aureus, streptococcus mitis, and pseudomonas aeruginosa to ultraviolet radiatio ...19911760141
secretory iga adsorption and oral streptococcal adhesion to human enamel and artificial solid substrata with various surface free energies.in this paper, secretory iga adsorption from a single component siga solution and from human whole saliva onto human enamel and artificial solid substrata with various surface free energies was studied as a function of time. elisa indicated that screening or displacement of adsorbed siga by other salivary proteins occurred only on low surface free energy substrata, not on high surface free energy substrata such as enamel. in addition, the adhesion of three oral streptococcal strains (streptococc ...19911772830
an h.p.l.c. method for determining chain-length distribution in some glycogens.human, oyster, streptococcus mitis, and phyto-glycogen samples were debranched using pseudomonas amylodermosa isoamylase (ec 3.2.1.68). the distribution of chain lengths was studied by high-performance liquid chromatography on reversed-phase columns, with water as eluent. quantitative data was obtained over the degree of polymerisation range three to eighteen (d.p. 3-18), and oligosaccharides up to d.p. 26 were detected. no single column was found suitable for the resolution of the complete rang ...19911786580
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
clinical and microbiological findings on newly inserted hydroxyapatite-coated and pure titanium human dental implants.the clinical and microbiologic features of 30 hydroxyapatite-coated root-form endosseous dental implants (tri-stage) were compared to 10 similar pure titanium implants without hydroxyapatite coatings. in 7 of 9 partially edentulous patients studied, pure titanium fixtures were placed adjacent to hydroxyapatite-coated implants. implants in the maxilla were submerged beneath mucosal tissues after implant placement for a minimum of 6 months, and in the mandible for at least 4 months. all patients w ...19911843465
inhibitory effects of human salivary histatins and lysozyme on coaggregation between porphyromonas gingivalis and streptococcus mitis.the effects of histatins on coaggregation between porphyromonas gingivalis 381 and streptococcus mitis atcc 9811 were investigated by using a turbidimetric assay. the coaggregation activity was significantly inhibited by histatins 5 and 8 and strongly by lysozyme. tritium-labeled histatin 8 bound to p. gingivalis cells but not to s. mitis cells.19911879942
postsepsis bradycardia in children with leukemia.we observed sinus bradycardia in a small number of children with hematologic malignancies who were recovering from sepsis. our objective was to define this symptom complex and attempt to delineate its etiology.19911884617
complete structure of the cell surface polysaccharide of streptococcus oralis c104: a 600-mhz nmr study.specific lectin-carbohydrate interactions between certain oral streptococci and actinomyces contribute to the microbial colonization of teeth. the receptor molecules of streptococcus oralis, 34, atcc 10557, and streptococcus mitis j22 for the galactose and n-acetylgalactosamine reactive fimbrial lectins of actinomyces viscosus and actinomyces naeslundii are antigenically distinct polysaccharides, each formed by a different phosphodiester-linked oligosaccharide repeating unit. these streptococci ...19911888724
subclass distribution of salivary secretory immunoglobulin a antibodies to oral streptococci.the ability of specific secretory immunoglobulin a (s-iga) antibodies to inhibit bacterial colonization of mucosal surfaces may be neutralized by the activity of bacterial iga1 proteases. because of the resistance of the iga2 subclass to these enzymes, the biological effect of iga1 proteases in vivo may depend on the subclass distribution of the bacterium-specific antibodies. we have estimated the subclass distribution of s-iga antibodies in saliva samples from 13 individuals against iga1 protea ...19911894364
[non-groupable streptococci: identification, sensitivity to antibiotics (charles nicolle hospital in tunis)].50 strains of viridans streptococci isolated from human material are identified by biochemical tests (bile esculin, cl na 6,5%, acid production from lactose, mannitol, sorbitol, inulin, arginine, esculin and starch hydrolysis; production of levan and dextran in sucrose media) streptococcus salivarius and streptococcus mitis are predominant species. susceptibility to antibiotics was studied: 70% of viridans streptococci were susceptible to all antibiotics tested, high level resistance to aminogly ...19911895917
[alpha-hemolytic streptococcal septicemia and meningitis in immunocompromised children].many kinds of microorganisms can produce toxic septicemia in immunocompromised hosts. we are reporting alpha-hemolytic streptococcal septicemia and meningitis in two children with hematological malignancies. [case 1] 6 year old girl who had been suffering from acute lymphocytic leukemia. she had sepsis and bacterial meningitis in maintenance-therapy for leukemia. streptococcus sanguis was isolated from the blood and cerebrospinal fluid (csf). [case 2] 11 year old girl who had had malignant lymph ...19911919121
[streptococcus mitis endocarditis. description of a clinical case].the diagnostic iter of a case of streptococcus mitis endocarditis is reported. bacterial endocarditis was diagnosed in a 32-year-old patient following ultrasound cardiography and microbiological tests. the paper stresses the importance of ultrasound cardiography in the diagnosis of bacterial endocarditis.19911922895
enumeration of oral streptococci on media containing different concentrations of sodium and potassium ions.laboratory "type" strains of oral streptococci were screened for their ability to grow on mitis-salivarius agar (msa) in the presence of increasing concentrations of either na+ or k+ up to 500 mmol/l. strains were generally better able to withstand increasing concentrations of na+ than k+, although low numbers of colony-forming units (cfus) were seen with the highest concentration of either cation. two strains of streptococcus mutans, ingbritt 162 and ingbritt 175, behaved differently when the c ...19911939833
ecology of viridans streptococci in the oral cavity and pharynx.recently published taxonomic studies of viridans streptococci have resulted in several changes in the nomenclature and definition of oral streptococcal species. with this background, the ecology of streptococci in the oropharyngeal cavities was reinvestigated. the results based on the examination of 1426 streptococcal isolates confirmed and extended earlier findings. apart from mature supragingival plaque, which contained a mixture of all orally encountered streptococci, each site showed a chara ...19911945494
[2 newborn infants with septicemia caused by multiresistant streptococcus mitis].multiresistant viridans streptococci had not yet been encountered in the netherlands. in the spring of 1990 we isolated multiresistant s. mitis from blood of two dutch neonates (minimal inhibitory concentration of penicillin 64 mg/l). in both patients septicaemia developed while they were being treated with beta-lactam antibiotics. although viridans streptococci usually are extremely sensitive to penicillin and other beta-lactams, the possibility of development of (multi-)resistance in these mic ...19911990312
effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria.chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ...19912005230
complete structure of the cell surface polysaccharide of streptococcus oralis atcc 10557: a receptor for lectin-mediated interbacterial adherence.lectin-carbohydrate binding is known to play an important role in a number of different cell-cell interactions including those between certain species of oral streptococci and actinomyces that colonize teeth. the cell wall polysaccharides of streptococcus oralis atcc 10557, s. oralis 34, and streptococcus mitis j22, although not identical antigenically, each function as a receptor molecule for the galactose and n-acetylgalactosamine reactive fimbrial lectins of actinomyces viscosus and actinomyc ...19912054351
salivary iga antibody to oral streptococcal antigens in predentate infants.salivary iga antibodies to oral streptococci which colonize the infant oral cavity soon after birth were analyzed in 53 whole salivas of 17 infants who were between three and 27 weeks old. antibody activities to streptococcus mitis cells were detected by enzyme linked immunosorbent assay in 78% of the whole salivas by the twelfth week of age. this antibody activity was associated with polymeric iga as determined after gel filtration of salivas on superose 6, followed by elisa. western blot analy ...19902087350
association of selected bacteria with the lesions of root surface caries.plaque from the root surfaces of 165 subjects (mean age 65.5 years, 22-26 teeth/subject) was analysed for specific bacteria. five subject groups were defined: a (dmfs 16.4), b (dmfs 55.9), c1 (dmfs 55.6), c2 (dmfs 57.0) and c3 (dmfs 48.1). groups c1 and c2 had unrestored root surface lesions; group a, b and c3 were free of unrestored root caries and differed in their coronal caries experience. streptococcus mutans was isolated more frequently from the root lesions in groups c1 and c2 than from i ...19902098715
selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene.the present investigation explored the hypothesis that elevated levels of certain enzymes in the gingival crevicular environment of individuals with poor oral hygiene and/or gingival inflammation may modify the surfaces of epithelial cells and thereby modulate the types of bacteria which attach and colonize. buccal epithelial cells treated with neuraminidase and certain proteases were used as a model for study. bacteria studied included streptococcus sanguis and streptococcus mitis which have be ...19902141877
[the use of teicoplanin in neutropenic patients: values and limits].the efficacy and the safety of teicoplanin were evaluated for the treatment of febrile episodes in neutropenic patients. a total of 18 patients received teicoplanin once daily as an intravenous injection of 200 mg in 6 patients (after a loading dose of 400 mg) or 400 mg in 12 patients (loading dose: 800 mg). the mean duration of therapy was 15.9 days (range 9 to 39 days). in all patients teicoplanin was combined with another antibiotic usually a beta-lactam. thirteen of 18 patients were successf ...19902143573
prevention of gram-positive and candida albicans infections using teicoplanin and fluconazole: a randomized study in neutropenic children.we have shown that the combination of teicoplanin (t) and ceftriaxone (c) given once daily as first-line empirical antimicrobial therapy is very effective (85%) and safe in febrile neutropenic children (icaac, 1988). like others (icaac, 1989) we have stressed the new life-threatening problems with streptococcus mitis and strep. sanguis. considering the regular sensitivity of gram-positive organisms to t, it is possible that the introduction of the glycopeptide at the onset of aplasia may be help ...19902149047
streptococcus mitis sepsis in bone marrow transplant patients receiving oral antimicrobial prophylaxis.streptococcal infection has increasingly become a problem in neutropenic patients. we report on an outbreak of streptococcus mitis sepsis in six bone marrow transplant patients receiving oral antimicrobial prophylaxis.19902171333
molecular aspects of immunoglobulin a1 degradation by oral streptococci.using a panel of 143 strains classified according to a novel taxonomic system for oral viridans-type streptococci, we reexamined the ability of oral streptococci to attack human immunoglobulin a1 (iga1) molecules with iga1 protease or glycosidases. iga1 protease production was an exclusive property of all strains belonging to streptococcus sanguis and streptococcus oralis (previously s. mitior) and of some strains of streptococcus mitis biovar 1. these are all dominant initiators of dental plaqu ...19902182537
effectiveness of ultrasonic files in the disruption of root canal bacteria.the physical mechanisms of ultrasound, namely cavitation and acoustic streaming, generated by the enac-osada ultrasonic unit were investigated for effectiveness in disrupting streptococcus mitis. in addition, the bactericidal effect of ultrasound in the presence of 2.5% sodium hypochlorite was examined. bacterial suspensions were irradiated directly with ultrasound in simulated root canals, and the viability of bacteria was examined after growth on a blood agar medium under anaerobic conditions ...19902216361
ability to bind salivary alpha-amylase discriminates certain viridans group streptococcal species.a collection of 144 viridans group streptococcal strains recently characterized as part of a taxonomic study was examined for the ability to bind salivary alpha-amylase. this property was found in most strains of streptococcus gordonii and streptococcus mitis and in occasional strains of streptococcus anginosus and streptococcus salivarius. in contrast, all strains of streptococcus sanguis, streptococcus oralis, streptococcus vestibularis, and streptococcus mutans lacked alpha-amylase-binding ca ...19902254435
comparison of the initial streptococcal microflora on dental enamel in caries-active and in caries-inactive individuals.this study compared the initial (4 h) microflora on enamel in 7 caries-active and in 7 caries-inactive adolescents. in both groups the microflora was dominated by streptococci which comprised 61 and 78% (median values) of the total viable counts in caries-active and caries-inactive individuals, respectively (p less than 0.01). identification of a total of 700 streptococcal isolates according to a recently revised classification showed that the predominant streptococci belonged to the species str ...19902276164
septicemia due to streptococcus mitis in neutropenic patients with acute leukemia.eight neutropenic patients with acute lymphocytic or nonlymphocytic leukemia had septicemia due to different strains of streptococcus mitis (st. mitis), a microorganism not commonly recognized as a special pathogen in leukemic patients. four of the patients had been treated with high-dose cytosine arabinoside as part of the cytostatic regimen, six had a central venous line and four patients had oral lesions prior to the infection. selective gut decontamination consisted of co-trimoxazole/colisti ...19902291985
microbiologic characteristics of lactobacillus products used for colonization of the vagina.lactobacilli have long been considered to be the protective flora in the vagina. women with vaginal infections have used various non-prescription products in an attempt to restore their normal vaginal flora. products that contain lactobacilli include dairy products (yogurt, acidophilus milk) and commercially available lactobacillus powders and tablets. recently, lactobacillus species that produce hydrogen peroxide (h2o2) have been associated with normal vaginal flora. in this study, we compared ...19902300352
cohesion between oral streptococci and neisseria pharyngis on saliva-coated glass, in the presence and absence of sucrose.ten strains of oral streptococci, comprising nine species, were screened for their in vitro cohesive interactions with a freshly isolated strain of neisseria pharyngis. strains of mutans streptococci, streptococcus salivarius, streptococcus sanguis i, streptococcus milleri and streptococcus mitis were able to cohere with n. pharyngis cells attached to a salivary pellicle adsorbed to glass. the cohesion was either due to coaggregation between the different genera or modification of the salivary p ...19902329945
microflora associated with experimental root surface caries in humans.this study describes the microflora from actively progressing root surface caries lesions, in which mineral loss had been determined by quantitative microradiography. the caries lesions were produced experimentally in root surface specimens from human molars inserted in lower partial dentures carried for 3 months by six elderly individuals. a total of 780 bacterial isolates were identified from 13 plaque samples, collected with a punch technique, and six dentin samples. the composition of the mi ...19902341169
adherence of mutans streptococci to other oral bacteria.adherence of mutans streptococci to strains of actinomyces viscosus, streptococcus sanguis, and streptococcus mitis immobilized on a nitrocellulose membrane was measured. strains of streptococcus mutans, s. sobrinus, and s. rattus bound in a lactose-independent manner to a variety of the actinomyces and streptococci. most of these reactions could proceed in the presence of whole saliva although adherence of s. rattus bht to the streptococci was inhibited by salivary molecules. in contrast, adher ...19902341174
inhibitory effect of human plasma and saliva on co-aggregation between bacteroides gingivalis and streptococcus mitis.the effect of human plasma and saliva on co-aggregation between bacteroides gingivalis and streptococcus mitis was studied by means of a turbidimetric assay. the co-aggregation activity was obtained from the maximum slope of the absorbance vs. time curve. its dependence on ph, temperature, and ionic strength was examined, and the number of bacteroides cells in relation to the number of streptococcus cells resulting in optimal co-aggregation was established. co-aggregation inhibition experiments ...19902384623
streptococcus mitis. a cause of serious infection in adults.twenty strains of streptococcus mitis were isolated from blood or body fluids at the cleveland veterans administration medical center from jan 1, 1981, to april 30, 1984. fifteen (75%) isolates were considered contaminants. five (25%) were clinically important and associated with a serious infection of the oropharynx or gastrointestinal tract (three of five), endovascular system (one of five), or a prosthetic hip. four of five patients required surgical intervention for treatment. two of five di ...19872437875
association of viridans group streptococci from pregnant women with bacterial vaginosis and upper genital tract infection.the prevalence and role of viridans group streptococci in the female genital tract have not been well described. in this study of 482 pregnant women, 147 (30%) were culture positive for viridans group streptococci. of 392 women with predominant lactobacillus morphotypes by gram stain (normal), 110 (28%) were colonized with viridans group streptococci, compared with 37 (41%) of 90 women with bacterial vaginosis (bv) (p = 0.02). to determine whether any species were associated with bv, 177 consecu ...19882454943
surface structures, co-aggregation and adherence phenomena of streptococcus oralis and related species.seven strains of streptococcus oralis were found to possess surface structures. four strains possessed long fimbriae which ranged in length from 266-366 nm, while the remaining three strains possessed shorter peritrichously distributed fibrils which ranged in length from 80-197 nm. the fibrillar strains were morphologically similar to strains of streptococcus sanguis i and ii and streptococcus mitis. no strain of s. oralis produced tufts of fibrils like certain strains of s. sanguis i. strains o ...19892475745
rapid detection of bordetella pertussis by a monoclonal antibody-based colony blot assay.monoclonal antibodies to bordetella pertussis filamentous hemagglutinin (fha) and lipopolysaccharide (lps) were used in a colony blot enzyme-linked immunosorbent assay designed for rapid detection of b. pertussis. bacterial colonies from bordet-gengou agar plates were blotted onto nitrocellulose filter disks, lysed by immersion in chloroform, and reacted with monoclonal antibodies. following reaction with peroxidase-conjugated rabbit anti-mouse immunoglobulin antisera and 4-chloro-1-naphthol, bl ...19892542357
a comparison between the elemental surface compositions and electrokinetic properties of oral streptococci with and without adsorbed salivary constituents.in order to characterize the functional cell surface, isoelectric points and elemental surface concentration, the ratios of nitrogen, oxygen and phosphorus to carbon of saliva-coated strains were determined by ph-dependent zeta potential measurements and x-ray photoelectron spectroscopy and compared with those of uncoated strains. the measurements of potential were carried out on completely hydrated cells, whereas the spectroscopy was on freeze-dried micro-organisms. the small increase in the ni ...19892610623
uptake of 14c-xylitol by xylitol-cultured streptococcus sobrinus atcc 27352 and streptococcus mitis atcc 36249 in vitro.the effect of three successive cultures in the presence of 6% xylitol on the uptake of 14c-xylitol was studied using resting cells of streptococcus sobrinus atcc 27352 and streptococcus mitis atcc 36249. in the case of s. mitis, the three successive cultures did not alter the growth inhibition observed in the presence of xylitol. in the case of s. sobrinus, however, growth inhibition decreased. the 14c-xylitol uptake experiments also demonstrated that uptake of xylitol by s. sobrinus was decreas ...19892633204
prophylactic co-trimoxazole versus norfloxacin in neutropenic children--perspective randomized study.co-trimoxazole or norfloxacin were randomly administered to 44 granulocytopenic children with malignancies in order to prevent bacterial infections. although more patients in the co-trimoxazole group had febrile episodes (p less than 0.01), the mean of febrile days and the mean of days with systemic antibiotics did not differ significantly in the two groups. five patients in the co-trimoxazole group had a microbiologically documented infection (four with septicemia) due to escherichia coli (n = ...19892654019
a double-blinded comparative evaluation of three media for chromophore testing with viridans and nutritionally variant (deficient) streptococci.a double-blinded prospective comparison of chromophore testing among 109 clinical isolates of alpha-hemolytic gram-positive cocci and 48 strains of nutritionally variant (deficient) streptococci (nvs) for its sensitivity, specificity, and predictive value under stimulated clinical laboratory conditions was performed after growth in three different media--todd-hewitt broth supplemented with 10 micrograms/ml of pyridoxal (thbp), thbp with 0.8% (w/v) yeast-extract (thbp + ye), and a semisynthetic c ...19892655426
molecular analysis of viridans and nutritionally deficient (variant) streptococci causing sequential episodes of endocarditis in a patient.clinical blood isolates from sequential episodes of endocarditis occurring over a six-month period of time in an addict were investigated. the pathogens were streptococcus sanguis ii, streptococcus mitis, and a nutritionally deficient (variant) streptococcus. the authors determined the dna relatedness of these isolates by antibiograms, plasmid profiles, chromosomal endonuclease restriction digestions, and dot blot dna-dna hybridization analyses. the s. sanguis ii and nutritionally deficient stre ...19892718964
molecular surface characterization of oral streptococci by fourier transform infrared spectroscopy.in order to characterize the molecular composition of oral streptococci, infrared transmission spectroscopy on freeze-dried cells dissolved in kbr was used. all infrared spectra show similar absorption bands for the strains studied with the most important absorption bands located at 2930 cm-1 (ch), 1653 cm-1 (ami), 1541 cm-1 (amii) and two bands at 1236 cm-1 and 1082 cm-1, which were assigned to phosphate and sugar groups. however, calculation of absorption band ratios normalized with respect to ...19892730916
effect of neuraminidase on the adherence to salivary pellicle of streptococcus sanguis and streptococcus mitis.neuraminidase-sensitive adherence to experimental salivary pellicles was studied using eight strains of streptococcus sanguis and five strains of streptococcus mitis. approximately 60% of the strains of each species showed significantly lower adherence to neuraminidase-treated versus untreated saliva-coated hydroxyapatite. in addition, the adherence of several of these streptococcal strains to saliva-coated hydroxyapatite and neuraminidase-treated saliva-coated hydroxyapatite was inhibited using ...19892736574
meningitis due to streptococcus mitis cross-reacting with streptococcus pneumoniae. 19892778344
[frequency and severity of systemic infections caused by streptococcus mitis and sanguis ii in neutropenic children].we have retrospectively evaluated 24 sepsis episodes caused by viridans streptococci in 23 neutropenic children during a 21 months period at the pediatric hematology unit of st. louis hospital. the underlying malignancies included acute lymphoblastic leukemia, acute non lymphoblastic leukemia, aplastic anemia and solid tumor. in 17 children neutropenia, defined as a neutrophil count of less than 500 per cubic millimeter, was caused by cytotoxic chemotherapy. for 6 other children neutropenia was ...19892780102
characterization of salivary alpha-amylase binding to streptococcus sanguis.the purpose of this study was to identify the major salivary components which interact with oral bacteria and to determine the mechanism(s) responsible for their binding to the bacterial surface. strains of streptococcus sanguis, streptococcus mitis, streptococcus mutans, and actinomyces viscosus were incubated for 2 h in freshly collected human submandibular-sublingual saliva (hsmsl) or parotid saliva (hps), and bound salivary components were eluted with 2% sodium dodecyl sulfate. by sodium dod ...19892788139
inhibition of adhesion of viridans streptococci to fibronectin-coated hydroxyapatite beads by lipoteichoic acid.fibronectin-coated hydroxyapatite (fnha) beads were used in a model adhesion assay to isolate the lipoteichoic acid (lta) mediated adhesion of oral streptococci. representative strains of the commonly isolated viridans streptococci were incubated with fnha beads in the presence and absence of exogenous lta. the lta inhibited the adhesion of all strains to a greater or lesser extent, but only a very few strains were inhibited by more than 90%. strains of streptococcus sanguis type ii and streptoc ...19882854117
neonatal meningitis due to streptococcus mitis.a baby girl became ill 2 days after a normal delivery. streptococcus mitis was isolated from the cerebrospinal fluid, and she recovered uneventfully on treatment with penicillin and, for the first 2 days, netilmicin. the organism was penicillin-tolerant in vitro. non-groupable alpha-hemolytic streptococci are an uncommon cause of meningitis; three cases have been ascribed to s. mitis.19892916097
[inoculation meningitis at streptococcus mitis]. 19882964015
isolation and characterization of a 60-kilodalton salivary glycoprotein with agglutinating activity against strains of streptococcus mutans.a bacterial agglutinin specific for strains of streptococcus mutans was isolated from human saliva. physiochemical analyses showed the agglutinin to be a glycoprotein with a molecular weight of 60,000. the agglutinin aggregated four of the eight strains of streptococcus mutans tested but did not aggregate the strains of streptococcus salivarius, streptococcus sanguis, and streptococcus mitis tested. chemical modification of carbohydrate moieties of the agglutinin with sodium metaperiodate had no ...19863002983
metal ion inactivation and chelator stimulation of streptococcus mitis arginine aminopeptidase.activation of streptococcus mitis (attc 9811) arginine aminopeptidase resulted in removal of the metal(s) from the enzyme molecule, and the action of the heavy metal ion in the inactivation process was shown to involve formation of a chelate complex between the enzyme molecule and metal or oxidation of functional group(s) on the enzyme surface. the enzyme also underwent activation by bovine serum albumin, amino acids, phosphate, and citric acid, which are probable physiological chelators.19873104766
interbacterial adhesion between pseudomonas aeruginosa and indigenous oral bacteria isolated from patients with cystic fibrosis.interbacterial adhesion between strains of pseudomonas aeruginosa and strains of indigenous oral bacteria, both of which were isolated from the oral cavity of cystic fibrosis patients, was investigated by the phenomenon of the coaggregation reaction. a total of 22 strains of p. aeruginosa were isolated from the oral cavity of 17 patients and examined for their abilities to coaggregate with 5 strains each of streptococcus sanguis, streptococcus mitis, actinomyces viscosus, and actinomyces naeslun ...19873105855
in-vitro adherence of oral streptococci in the presence of sucrose and its relationship to cariogenicity in the rat.streptococcus sanguis i gave a significantly greater percentage coverage (cell-pellicle attachment) of saliva-coated glass in the presence of sucrose than did strep. sanguis ii (p less than 0.025), and both these gave greater percentage coverages than the other species tested, between which no significant differences were noted. there was a large number of significant differences in clump size (cell-cell attachment) between species-pairs. among the mutans streptococci, there were significant dif ...19883166609
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